Shodex Catalogue 2013-2015.pdf - Analytics Shop

TMWe provide a wide range of products to meet your analytical needs, from pretreatment andseparation columns to calibration standards for size exclusion chromatography.Please visit the Shodex website to see application data and other useful info.Shodex websitewww.shodex.deThe following names are trademarks or registered trademarks of SHOWA DENKO K.K.Shodex, AFpak, Asahipak, AXpak, CLNpak, CXpak, MSpak, ODP, ODSpak, OHpak, ORpak, RSpak, Silicapak,SUGAR, USPpak[Caution]1. Please read the operating manual included in the product box carefully before use.2. For improvement purposes, some specifications might change without notice.3. The figures and descriptions in this catalogue are not guaranteed and do not warrant suitability for your applications.4. It is essential to take normal precautions when handling reagents and other chemical products even if the safetyinformation is not included in the operating manual.5. Products described in this brochure are not intended for medical use or medical applicationsincluding medical diagnosis.

HPLC Separation ModesLiquid chromatography (LC) uses liquid as mobile phase (eluent). It is an analytical method that separates a mixture of compoundsbased on their physical and chemical differences. High performance liquid chromatography (HPLC) is a method that introducesthe mobile phase under high-pressure conditions that results in rapid and high-performance separations. The various interactionsbetween the analyte, stationary phase (packing material), and mobile phase are the key factors for the separation. A wide varietyof separation mechanism can be obtained by using particular combinations of stationary and mobile phases.Separation modeReversed PhaseChromatography(RPC)HydrophilicInteractionChromatography(HILIC)Normal PhaseChromatography(NPC)Ligand ExchangeChromatography(LEX)Ion ExclusionChromatography(IEX)IonChromatography(IC)Size ExclusionChromatography(SEC)Ion ExchangeChromatography(IEC)HydrophobicInteractionChromatography(HIC)AffinityChromatography(AFC)Chiral SeparationChromatography(CS)MultimodeChromatography• Separation is based on hydrophilic interaction.• A high polarity stationary phase is used.• Typically the mobile phase contains a mixture of organic solvents such as acetonitrile and aqueous solvents (water or buffer).• Using the higher polarity mobile phase causes a faster elution.• Applicable for the analysis of high polar substances.• Separation is based on the partition equilibrium between the stationary phase and the mobile phase.• The polarity of the stationary phase is higher than that of the mobile phase.• Typically the mobile phase contains a mixture of organic solvents with different polarities such as hexane and isopropanol.• Using the higher polarity mobile phase causes a faster elution.• Separation is based on differences in analytes’ coordination complex.• Stationary phase modified with metal sulfonate complex ion.• Works in combination with size exclusion or HILIC modes.• Separation is based on electrostatic interaction (repulsion) between the ion exchanger and ionic solutes.• Dissociated ionic molecules elute faster than non-dissociated forms.• Used mainly for the analysis of organic acids.• Separation is based on electrostatic interaction (bonding) between the ion exchanger and ionic solutes.• Has a relatively small ion exchange capacity.• Electrical conductivity detector can be used with low-salt concentration mobile phase.• Used mainly for the analysis of inorganic compounds.• Network or pores on the surface of the packing material work as molecular sieve to separate molecules based on their sizes.• To separate molecules solely based on their sizes, it requires an analytical condition without any analyte and packing gelinteraction.• The bigger the molecule size, the faster the elution sequence.• Used for molecular weight or molecular distribution determination of macromolecules and qualification of oligomers.• Separation is based on electrostatic interactions between the ion exchanger and ionic solutes.• The mobile phase of choice should have a sufficient buffering capacity at the pH that produces the largest chargedifferences between the analyte of interest.• The elution position is optimized by varying the pH, salt concentration, and/or ionic strength of the mobile phase.• Separation is based on hydrophobic interaction.• Hydrophobic functional group is modified on the stationary phase.• Adsorption of analytes generally occurs at a high salt concentration and they are released by lowering the salt concentration.• Used mainly for the analysis of proteins.• Separation is based on adsorption of the analyte to the specific biologically derived ligand pair.• Highly selective.• A buffer solution with the appropriate pH and ionic strength is selected based on the type of ligand, analytes, and their interaction.• Used mainly for the purification and concentration of biological active substances.• Separation of optical isomers using chiral selectors.• Highly selective.• Separation is based on the combination of different modes.Characteristics• Separation is based on the partition equilibrium between stationary phase and mobile phase.• The polarity of the stationary phase is lower than that of the mobile phase.• Typically the mobile phase contains a mixture of organic solvents (methanol, acetonitrile, or THF) and aqueous solvents(water or buffer).• Using the lower polarity mobile phase causes a faster elution.Column Selection by Sample Character andSeparation ModeColumn Selection HPLC Separation Modes Column Selection by Sample Character and Separation ModeSampleSolubilityAqueoussolubleSampleMW≥ 2,000≤ 2,000SeparationModeRPCLEXIEXSECIECHICAFCRPCHILICLEXIEXICSECIECAFCCSSampleSolubilityOrganicsolubleSampleMW≥ 2,000≤ 2,000SeparationModeSECRPCNPCSECRPC : Reversed Phase ChromatographyHILIC : Hydrophilic Interaction ChromatographyNPC : Normal Phase ChromatographyLEX : Ligand Exchange ChromatographyIEX : Ion Exclusion ChromatographyIC : Ion ChromatographySEC : Size Exclusion ChromatographyIEC : Ion Exchange ChromatographyHIC : Hydrophobic Interaction ChromatographyAFC : Affinity ChromatographyCS : Chiral Separation Chromatography03

BiotechnologyGenomicsProteomicsGlycomicsNucleobasesNucleotidesNucleosidesOligo nucleicacidsDNA, RNAAmino acidsPeptidesProteinsGlycoproteinsSugar chainsSeparation ModeRPCIEC+SECIECRPCIEC+SECIECSECRPCIEC+IEX+RPCIECIEC+SECRPCSECIECHICRPCSECIECHICAFCHILICAFCPage10, 5810, 32, 585210, 58325230, 348, 5810, 5824, 54328, 10, 5828,30,32,3452, 54568, 10, 5828,30,32,3452, 54565612, 5856EnvironmentWater qualitySoilEnvironmentalhormonesAnionsOxyhalidesCyanideCyanogen chlorideCationsSurfactantsPerchloric acidsPesticidesAnionsHeavy metalsHumic substancesOrganic arsenicPesticidesPretreatment ofPhthalatesPCBsBenzo[a]pyreneSeparation ModeICICIECIEXICRPCSEC+RPCICIECRPCIEC+RPCICICICSECIEX+RPCRPCIEC+RPCICSECGPCClean-up( )Page22225820248, 1434, 58225810, 5810, 582222243010, 581010, 582262Column Selection Column Selection (Application) • Biotechnology • EnvironmentMonosaccharidesHILICLEX+SEC12, 5816MonosaccharidesOligosaccharidesHILICLEX+SEC12, 5816HormoneSialic acidsUronic acidsAldonic acidsAminesLEX+HILICIEX+SECRPCIECRPC16206, 8, 10, 58548, 58BioethanolsOligosaccharidesAlcoholsFurfuralSaccharidesOrganic acidsAlcoholsFurfuralHemicellulosesCellulosesLEX+SECIEX+RPC+SECSEC162040, 44SteroidsHILIC12, 58CationsIC24LipidPhospholipidsLipoproteinsSECNPCSECSECAFC30, 34, 581434,36,42,583056BiodieselsFatty acid glyceridesFatty acid methyl estersOrganic acidsSECRPCIC34, 58102205

Standard AnalysisColumnsColumns for Polymer-based Reversed Phase ChromatographyColumns for Polymer-basedReversed Phase ChromatographyFeaturesODP2 HPStandard columnsProduct CodeF7622001F7622002F7622003F6714010F7622004F7622005F6714011Provides nearly twice as large theoretical plate number compared with generally availablepolymer-based reversed phase columnsColumn offers enhanced retention of high polar substances compared with ODS columnSuitable for reversed phase analysis of small molecules such as pharmaceutical compounds in thepresence of protein matrixIdeal for LC/MS analysis of high polar compoundsNotebook No.6 News No.30, 36See also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction andnormal phase chromatography".Product NameODP2 HP-4BODP2 HP-4DODP2 HP-4EODP2 HPG-4AODP2 HP-2BODP2 HP-2DODP2 HPG-2APlate Number(TP/column)≥ 3,500≥ 13,000≥ 17,000(guard column)≥ 3,000≥ 7,000(guard column)FunctionalGroup−−−−−−−Particle Size(µm)5555555Pore Size(Å)404040−4040−Column Size(mm)I.D. x Length4.6 × 504.6 × 1504.6 × 2504.6 × 102.0 × 502.0 × 1502.0 × 10Shipping SolventH 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=55/45Base Material : PolyhydroxymethacrylateTolerance of ODP2 HP for alkaline conditionComparison of chromatograms obtained beforeand after passage of alkaline eluentODP2 HP-4DBefore passageAfter 500 hrpassage1 ODS10 5 10min2Before passageAfter 24 hrpassageSample : 5μL1. Pyridine 200μg/mL2. Phenol 430μg/mL0 5 10min2Relative theoretical plate number for pyridineCorrelation between alkaline eluentpassage time and relative theoreticalplate number(%)1201008060402000ODP2 HP-4DODS200 400 600Alkaline eluent passage time (hr)ConditionColumn : Shodex ODP2 HP-4DODS from other manufacturerEluent : H 2 O/CH 3 OH=70/30Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 40˚CEluent passage conditions for alkali tolerance testEluent : 10mM Sodium phosphate buffer(pH12)/CH 3 CN=45/55Flow rate : 0.6mL/minColumn temp. : 30˚C06*Contact Shodex or our distributors near you for customized columns.

Comparison between ODP2 HP and ODP-50 (existing)Sample : 5μL1. Phenol 300mg/L2. Methyl benzoate 350mg/L3. Toluene 1000mg/L4. Naphthalene 150mg/L123ODP2 HP-4DTP:11,400412ODP-50 4D (existing)3TP:5,700ImidazolesSample : 0.1% each, 10μL1. Imidazole2. 2-Methylimidazole3. 4-Methylimidazole123Standard AnalysisColumns40 5 10 min 0 5 10 min 0510 minColumn : Shodex ODP2 HP-4DEluent : H 2 O/CH 3 CN=55/45Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 40˚CRatio of Pressure (%)160150140130120110ODSColumn : Shodex Asahipak ODP-50 4DEluent : H 2 O/CH 3 CN=35/65Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 40˚CInfluence of repeated protein injection on column pressureColumn : Shodex ODP2 HP-4EEluent : 10mM Na 2 HPO 4 aq./CH 3 CN=90/10Flow rate : 0.8mL/minDetector : UV(220nm)Column temp. : 40˚CODP2 HP series column is packed with gels having increasedsurface polarity and small pore size. This enables to remove andprevent adsorption of proteins. BSA was injected multiple timesto an ODS and an ODP2 HP columns. A significant columnpressure increase was observed with the ODS column, while noconsiderable change was observed with the ODP2 HP columneven after 140 injections.Sample : 7.0mg/mL BSA, 5μLColumns for Polymer-based Reversed Phase Chromatography100900ODP2 HP-2B20 40 60 80 100 120 140Number of InjectionTaking original pressure as 100%Column : Shodex ODP2 HP-2BODS from other manufacturerEluent : 1mM Ammonium acetate aq./CH 3 CN=90/10Flow rate : 0.2mL/minDetector : UV(220nm)Column temp. : 30˚CSimultaneous analysis of multi symptom cold formula in serum22Standards5Sample : 10μL1. Dihydrocodeine phosphate2. Acetaminophen3. Aspirin4. Salicylamide5. Salicylic acid6. Bromhexine hydrochloride7. Ibuprofen1Blood serum(without pretreatment)3Sample : Human serum, 10μL1. Dihydrocodeine phosphate2. Acetaminophen3. Aspirin4. Salicylamide5. Salicylic acid34167450 10 20 30 40 50 60 min0 10 20 30 40 50 minColumn : Shodex ODP2 HP-4EColumn : Shodex ODP2 HP-4EEluent : 0.1% TFA aq./CH 3 CN=70/30Eluent : 0.1% TFA aq./CH 3 CN=70/30Flow rate : 0.4mL/minFlow rate : 0.4mL/minDetector : UV(235nm)Detector : UV(235nm)Column temp. : 40˚CColumn temp. : 40˚CData courtesy of Katsuko Hara.MT Yutaka Komiyama.Ph.D., Department of Clinical Sciences and Laboratory Medicine, Kansai Medical University.07

Standard AnalysisColumnsColumns for Polymer-basedReversed Phase ChromatographyFeaturesODP-50, C8P-50, C4P-50Relative large pore size is suitable for the analysis of amino acids, peptides, and proteinsUsable in a wide pH range from acidic to alkaline (pH 2 to 13)Usable in 100% water or buffer solutionBest used for the analysis of basic substancesColumns for Polymer-based Reversed Phase ChromatographyODP-40Standard columnsProduct CodeF7621001F7621002F7620002F7620001F6710001F6710023F7620004F7620003F6710022F7620009F6713001F7620006Product NameNewsNo.35Higher performance type of ODP-50 seriesNewsAsahipak ODP-40 4DAsahipak ODP-40 4EAsahipak ODP-50 6DAsahipak ODP-50 6EAsahipak ODP-50G 6AAsahipak ODP-50 4BAsahipak ODP-50 4DAsahipak ODP-50 4EAsahipak ODP-50G 4AAsahipak ODP-50 2DAsahipak ODP-50G 2AAsahipak C8P-50 4DNo.1Plate Number(TP/column)≥ 11,000≥ 17,000≥ 9,000≥ 14,000(guard column)≥ 2,500≥ 9,000≥ 14,000(guard column)≥ 5,000(guard column)≥ 7,000FunctionalGroupOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctylParticle Size(µm)445555555555Pore Size(Å)250250250250−250250250−250−250Column Size(mm)I.D. x Length4.6 × 1504.6 × 2506.0 × 1506.0 × 2506.0 × 104.6 × 504.6 × 1504.6 × 2504.6 × 102.0 × 1502.0 × 104.6 × 150PreparativeColumnsSemi-microMicro ColumnsShipping SolventH 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65p.78p.72F7620005Asahipak C8P-50 4E≥ 11,000Octyl52504.6 × 250H 2 O/CH 3 CN=35/65F6710002Asahipak C8P-50G 4A(guard column)Octyl5−4.6 × 10H 2 O/CH 3 CN=35/65F7620008Asahipak C4P-50 4D≥ 6,000Butyl52504.6 × 150H 2O/CH 3CN=35/65F7620007Asahipak C4P-50 4E≥ 9,000Butyl52504.6 × 250H 2 O/CH 3 CN=35/65F6710003Asahipak C4P-50G 4A(guard column)Butyl5−4.6 × 10H 2 O/CH 3 CN=35/65Base Material : Polyvinyl alcoholFeatures and applications of different packing materials used for columns for reversed phase,hydrophilic interaction and normal phase chromatographyLargePore size of packing materialsSilicapakSILSaccharides,Sugar alcohols,Hydrophilic low molecularweight substancesDM-614PolycyclicaromaticcompoundsC4P-50C8P-50Peptides5NH 5CN 5C4 5C8 ODSNH2P-40,50ODP2 HPDEProteinsODP-40,50RP18-415DS-413,613VitaminsSmallLow molecular weight organic compounds,drugs, organic acids, food additives, etc.08HighPolarity of packing materialsLow*Contact Shodex or our distributors near you for customized columns.

AlkylalcoholsC8C6C4EGC6C10C12C14C8C4EG C10 C12C4P-50C8P-50Elution volume (mL)15105Sample : Ethyleneglycol, n-AlkylalcoholODP-50C8P-50C4P-50Tolerance of ODP-50 for alkaline condition123Sample :1. Acetophenone2. Butyrophenone3. Hexanophenone4. Heptanophenone5. Octanophenone45330hrStandard AnalysisColumnsC6 C4EG0 5231C8C10Local anesthetics0 20 minC14ODP-50C12C1410 15 20 25 minpH3 pH7 pH111230 20 minColumn : Shodex AsahipakODP-50 4DEluent : 25mM Phosphate buffer/CH 3 CN=60/40Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚C2 4 6 8 10 12 14Carbon number (n)Relationship between the carbon number ofalkylalcohols and elution volumeColumn : Shodex Asahipak ODP-50 4DAsahipak C8P-50 4DAsahipak C4P-50 4DEluent : H 2 O/CH 3 OH=20/80Flow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CDissociation of tertiary amino groups in basic drugs can be suppressed by making pH of the eluenthigher than pKa of the amino groups. This increases the relative hydrophobicity of the basic drugs,thereby allowing the column to retain the drugs stronger and provide baseline separation of them.10 20 min23Sample :1. BenzocaineH2N2. LidocaineCH3CH33. TetracaineNHCOCH2NCOOCH2CH2NNH(CH2)3CH3COOC2H5C2H5C2H5CH3CH30 10 20 min120hr90hrSample : 20μL1. Heptaoxyethylenedodecylether(PAR-Co) 1μg/L2. PAR230hrColumn : Shodex Asahipak ODP-50 4DEluent : 10mM NaOH aq.(pH12.0)/CH 3 CN=35/65Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚CNonionic surfactants0 1 2 3 4 5 6 7minColumn : Shodex Asahipak ODP-40 4DEluent : 10mM Sodium tetraborate aq./CH 3 OH=62/38Flow rate : 0.8mL/minDetector : VIS(510nm)Column temp. : 40˚CColumns for Polymer-based Reversed Phase ChromatographyFat-soluble vitamins Macrolide antibiotics Gradient analysis of proteins and peptides21Sample : 20μL1. Vitamin K3 1.5μg/mL2. Vitamin A 1.0 IU/mL3. Vitamin A acetate 0.5 IU/mL4. Vitamin D2 13.2μg/mL5. Vitamin D3 13.2 IU/mL6. Vitamin E acetate 2.4μg/mL7. Vitamin E 2.5μg/mL8. Vitamin K1 2.4μg/mL5481Sample : 0.1% each, 10μL1. Erythromycin2. Azithromycin25642 3120%CH3CN891071112Sample : 40μL12345678950%CH3CNSample Mw Recovery(%)Lys-Bradykinin 1188 97Bradykinin 1060 92Met-Enkephalin 574 97Neurotensin 1673 99Leu-Enkephalin 556 100Substance P 1348 93Bacitracin 1450 81Insulin5750 95Insulin B chain 3496 913671011LysozymeMastoparan143001479969612Myoglobin175008305 10 15 20 25min0 10 20 min0 510 15 20 25minColumn : Shodex Asahipak ODP-50 4EEluent : CH 3 CN/CH 3 OH=50/50Flow rate : 0.6mL/minDetector : UV(280nm)Column temp. : 30˚CColumn : Shodex Asahipak ODP-40 4EEluent : 40mM Potassium phosphatebuffer(pH11.0)/CH 3 CN=40/60Flow rate : 0.5mL/minDetector : UV(223nm)Column temp. : 40˚CColumn : Shodex Asahipak ODP-50 6DEluent : (A); 0.05% TFA aq./CH 3 CN=80/20(B); 0.05% TFA aq./CH 3 CN=50/50Linear gradient; (A) to (B), 20minFlow rate : 1.0mL/minDetector : UV(220nm)Column temp. : 30˚C09

Standard AnalysisColumnsColumns for Polymer-basedReversed Phase ChromatographyFeaturesRP18-415DS-613, 413Large pore size is suitable for the analysis of proteins and peptidesSuitable for reversed phase analysis of highly hydrophilic substances, whichcannot be retained well by ODS columnsColumns for Polymer-based Reversed Phase ChromatographyDEDM-614NNJJ-50F7009000NewsStandard columnsRSpak RP18-415No.14, 23, 24, 35Suitable for the analysis of amino acids and water-soluble vitaminsNewsNo.7See also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction andnormal phase chromatography".Product CodePolymer-based columns, with similar polarity to that of ODS columns, can beused in general and wide purposeWide working pH range (2-12), usable in 100% water and buffer solutionsThe packing material contains sulfo groups, and supports multimode (reversedphase and cation exchange) analysisIdeal for analysis of complex samples containing neutral and ionic substancesNotebookNo.3The packing material contains trace amounts of quaternary ammonium groups, andsupports multimode (reversed phase and anion exchange) analysisIdeal for analysis of complex samples containing neutral and ionic substancesProduct NameNews≥ 5,000No.7Plate Number(TP/column)FunctionalGroup−ParticleBase Material Size(µm)Styrene divinylbenzene copolymer 6PoreSize(Å)450Column Size(mm)I.D. x Length4.6 × 150Semi-microMicro ColumnsPreparativeColumnsPreparativeColumnsSemi-microMicro ColumnsShipping SolventH 2 O/CH 3 CN=5/95p.72p.78p.78p.72F6709558RSpak RP18-G(guard column)−Styrene divinylbenzene copolymer6−4.6 × 10H 2 O/CH 3 CN/THF=40/30/30F7001001RSpak DS-613≥ 6,500−Styrene divinylbenzene copolymer62006.0 × 150H 2 O/CH 3 CN/THF=30/40/30F7001012RSpak DS-413≥ 11,000−Styrene divinylbenzene copolymer3.52004.6 × 150H 2 O/CH 3 CN/THF=40/30/30F6700140RSpak DS-G(guard column)−Styrene divinylbenzene copolymer10−4.6 × 10H 2 O/CH 3 CN/THF=30/40/30F7001004RSpak DE-613≥ 7,000−Polymethacrylate6256.0 × 150H 2 OF7001005RSpak DE-413≥ 11,000−Polymethacrylate4254.6 × 150H 2 O/CH 3 CN=50/50F7009030RSpak DE-413L≥ 17,000−Polymethacrylate4254.6 × 250H 2 O/CH 3 CN=50/50F6700150RSpak DE-G(guard column)−Polymethacrylate10−4.6 × 10H 2 OF7001007RSpak DE-213≥ 8,000−Polymethacrylate4252.0 × 150H 2 O/CH 3 CN=50/50F6700151RSpak DE-SG(guard column)−Polymethacrylate6−2.0 × 10H 2 O/CH 3 CN=50/50F7001002RSpak DM-614≥ 4,500−Polyhydroxymethacrylate102006.0 × 1505mM H 3 PO 4 aq.F6700160RSpak DM-G(guard column)−Polyhydroxymethacrylate12−4.6 × 105mM H 3 PO 4 aq.F7008140RSpak NN-814≥ 9,000SulfoPolyhydroxymethacrylate102008.0 × 2500.1M Sodium phosphate buffer(pH3.0)F7008150RSpak NN-614≥ 4,000SulfoPolyhydroxymethacrylate102006.0 × 1500.1M Sodium phosphate buffer(pH3.0)F6700510RSpak NN-G(guard column)SulfoPolyhydroxymethacrylate10−6.0 × 500.1M Sodium phosphate buffer(pH3.0)F7008160RSpak NN-414≥ 6,000SulfoPolyhydroxymethacrylate102004.6 × 1500.1M Sodium phosphate buffer(pH3.0)10F7008240F7008220RSpak JJ-50 4DRSpak JJ-50 2D≥ 4,500≥ 3,500QuaternaryammoniumQuaternaryammoniumPolyvinyl alcoholPolyvinyl alcohol551001004.6 × 1502.0 × 150H 2 O/CH 3 CN=40/60H 2 O/CH 3 CN=40/60*Contact Shodex or our distributors near you for customized columns.

Separation and recovery rate of standardproteins123456Recovery (%)1. Ribonuclease A 932. Insulin983. Cytochrome c 1004. Lysozyme 1005. BSA986. Myoglobin 1087. Ovalbumin —7Alkylbenzenes21Sample : 5μL1. m-Cresol 0.1%2. 2,4-Xylenol 0.1%3. Benzene 0.5%4. Toluene 0.5%5. Ethylbenzene 0.5%6. n-Propylbenzene 0.5%345Fatty acid methyl esters1Sample : 0.2% each, 20μL1. Methyl linoleate2. Methyl palmitate3. Methyl oleate4. Methyl stearateStandard AnalysisColumns26340 10 20 30 minColumn : Shodex RSpak RP18-415Eluent : (A); 0.1% TFA aq./CH 3 CN=99/1(B); 0.1% TFA aq./CH 3 CN=5/95Linear gradient; 20%(B) to 60%(B), 20minFlow rate : 1.0mL/minDetector : UV(220nm)Column temp. : Room temp.Organic acids213456Sample : 5μL1. Glyoxylic acid 1.78mg/mL2. Tartaric acid 1.95mg/mL3. Malic acid 2.06mg/mL4. Lactic acid 2μL/mL5. Malonic acid 1.95mg/mL6. Acetic acid 2μL/mL7. Succinic acid 2.05mg/mL8. Levulinic acid 1.95mg/mL9. Propionic acid 2μL/mL789012 4 6 8 10 minColumn : Shodex RSpak DS-613Eluent : H 2 O/CH 3 CN/THF=30/40/30Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 40˚CFood additives (Preservatives)52346Sample : 10μL1. Saccharin sodium 0.005%2. p-Hydroxybenzoic acid 0.005%3. Sorbic acid 0.02%4. Benzoic acid 0.02%5. Methyl paraben 0.01%6. Dehydroacetic acid 0.01%7. Ethyl paraben 0.02%8. Propyl paraben 0.02%780 5 10 minColumn : Shodex RSpak DS-413Eluent : H 2 O/CH 3 CN/THF=25/45/30Flow rate : 1.0mL/minDetetor : RIColumn temp. : 40˚CDiolsn=2345Sample : 1% each, 7.5μLHO(CH 2 )nOH6Columns for Polymer-based Reversed Phase Chromatography0 1 2 3 4 5 6 7 8 minColumn : Shodex RSpak DE-413Eluent : 10mM H 3 PO 4 aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 50˚C0 5 10 15 20 min 0 5 10 15 minColumn : Shodex RSpak DE-413Column : Shodex RSpak DE-613Eluent : 50mM KH 2 PO 4 + 0.1% H 3 PO 4 aq. Eluent : H 2 O/CH 3 CN=65/35Flow rate : 1.0mL/minFlow rate : 1.0mL/minDetector : RIDetector : UV(210nm)Column temp. : 60˚CColumn temp. : 40˚CCarnitineAmino acidsPhenylenediamine isomersSample : 20μL1. L-Carnitine 0.1%1Sample : 0.1% each, 20μL1. Aspartic acid2. Glycine3. Alanine4. Valine5. Methionine6. Isoleucine1Sample : 100mg/L each, 20μL1. p-Phenylenediamine2. m-Phenylenediamine3. o-Phenylenediamine2N +OH OOH12343560 5 10 15 20 25min15 20 25 30 35 40 45 50 55min0 5 10 15 minColumn : Shodex RSpak NN-814Eluent : 0.1M H 3 PO 4 aq.Flow rate : 1.0mL/minDetector : UV(210nm)Column temp. : 25˚CColumn : Shodex RSpak NN-814Eluent : 40mM H 3 PO 4 aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex RSpak JJ-50 4DEluent : 25mM Ammonium acetate buffer(pH9.2)/CH 3 CN=70/30Flow rate : 0.4mL/minDetector : UV(254nm)Column temp. : 30˚C11

Standard AnalysisColumnsColumns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)Columns for Polymer-basedHydrophilic Interaction Chromatography (HILIC)FeaturesNH2P-50NH2P-40 3ESee also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction andnormal phase chromatography".Standard columnsProduct CodeF7630005F7630002F7630001F6710016F7630006F6713000F7630007F6710030F6710100Suitable for saccharides analysis by hydrophilic interaction chromatography (HILIC)Polymer-based packing material provides excellent chemical stability and minimumdeterioration over extended time periodWashable with alkaline solutionAlso suitable for evaporative light scattering detector, corona charged aerosol detector,and LC/MSNotebook No.2 News No.6, 14, 17, 32Provides higher theoretical plate number compared to NH2P-50 seriesAchieves 2-3 times improved detection sensitivity even with a conventional HPLC systemUses less than 50% solvent compared to NH2P-50 4ENewsNo.44Product NameAsahipak NH2P-50 4BAsahipak NH2P-50 4DAsahipak NH2P-50 4EAsahipak NH2P-50G 4AAsahipak NH2P-50 2DAsahipak NH2P-50G 2AAsahipak NH2P-40 3EAsahipak NH2P-50G 3AAsahipak NH2P-LFPlate Number(TP/column)≥ 1,500≥ 5,500≥ 7,500(guard column)≥ 3,500(guard column)≥ 8,500(guard column)(line filter)FunctionalGroupAminoAminoAminoAminoAminoAminoAminoAminoAminoParticle Size(µm)55555545−Pore Size(Å)Comparison of polymer-based (NH2P-50) and silica-based amino columns100100100−100−100−−Column Size(mm)I.D. x Length4.6 × 504.6 × 1504.6 × 2504.6 × 102.0 × 1502.0 × 103.0 × 2503.0 × 108.0 × 75PreparativeColumnsSemi-microMicro ColumnsShipping SolventH 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75p.79p.72Base Material : Polyvinyl alcoholFirst use123NH2P-50 4EAfter 180hr132Silica-based amino columnFirst useAfter 120hr1Polymer-based amino column, NH2P-50,showed highly repeatable result (bothretention time and peak shapes) for allsaccharides tested; proving its hightolerance to time degradation.41. Fructose2. Glucose3. Sucrose4. Maltose42311. Fructose2. Glucose2 33. Sucrose4 4. Maltose4120 10Ve (mL)201816141210820 min 0 10 20 min 0 10 20 min 0 10 20 min50 100 150 200Time (hr)MaltoseSucroseGlucoseFructoseVe (mL)201816141210850 100 150 200Time (hr)MaltoseSucroseGlucoseFructoseColumn : Shodex Asahipak NH2P-50 4ESilica-based amino columnfrom other manufacturerEluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 30˚C*Contact Shodex or our distributors near you for customized columns.

Fructooligosaccharide syrup2Sample :Fructooligosaccharide syrup, 2.5%, 20μL1. Fructose2. Glucose3. Sucrose4. 1-Kestose5. Nystose6. 1-Fructofuranosyl-D-nystoseChitooligosaccharidesSample : Chitooligosaccharides 2%, 20μL1. D-Glucosamine2. Chitobiose hydrochloride3. Chitotriose hydrochloride4. Chitotetraose hydrochloride5. Chitopentaose hydrochloride6. Chitohexaose hydrochloride1Oligogalacturonic acids34Sample : Oligogalacturonic acids1. Galacturonic acid2. Oligogalacturonic acid dimer3. Oligogalacturonic acid trimer4. Oligogalacturonic acid tetramer5. Oligogalacturonic acid pentamer6. Oligogalacturonic acid hexamerStandard AnalysisColumns10 501321041215615 20 25minColumn : Shodex Asahipak NH2P-50 4EEluent : H 2 O/CH 3 CN=30/70Flow rate : 1.0mL/minDetector : RIColumn temp. : 25˚CSaccharide analysis using corona charged aerosol detectorSample : 40μg/mL each, 5μL1. Fructose2. GlucoseSilica-based amide column(4.6mmI.D. x 150nm)5 10 15 minSilica-based amino column(4.6mmI.D. x 250nm)2NH2P-50 4E(4.6mmI.D. x 250nm)Column : Shodex Asahipak NH2P-50 4ESilica-based amino column from other manufacturerSilica-based amide column from other manufacturerEluent : H 2 O/CH 3 CN=20/80Flow rate : 1.0mL/minDetector : Corona charged aerosolColumn temp : 30˚C(NH2P-50 4E, Silica-based amino column)80˚C(Silica-based amide column)230 5 10 15 minColumn : Shodex Asahipak NH2P-50 4EEluent : H 2 O/CH 3 CN=30/70Flow rate : 1.0mL/minDetector : RIColumn temp. : 25˚CA corona charged aerosoldetector measures analytes asparticles. Accordingly, thebaseline will be significantlyinfluenced by componentseluted from the column.The polymer-based aminocolumn, NH2P serieseliminates column originatedcomponents, and thus enablesa stable baseline with lowernoise level.456Pyridylaminated monosaccharides1231250 5 10 15 20 minColumn : Shodex Asahipak NH2P-50 4EEluent : 0.3M Sodium phosphate buffer(pH4.4)Flow rate : 1.0mL/minDetector : UV(210nm)Column temp. : 40˚CSample offered by Prof. Yoshino in Kyoto Women's Univ.0 20 40 60 minColumn : Shodex Asahipak NH2P-50 4EEluent : H 3 PO 4 /H 2 O/CH 3 CN=1/14/85Flow rate : 0.5mL/minDetector : Fluorescence(Ex. : 310nm, Em. : 380nm)Column temp. : 40˚C46Sample : 5μL1. 2-Aminopyridine 0.1pmol/μL2. PA-Rhamnose 1pmol/μL3. PA-Fucose 1pmol/μL4. PA-Ribose 1pmol/μL5. PA-Xylose 1pmol/μL6. PA-N-Acetylglucosamine 1pmol/μL7. PA-N-Acetylgalactosamine 1pmol/μL8. PA-Mannose 1pmol/μL9. PA-Glucose 1pmol/μL10. PA-Galactose 1pmol/μL5678910Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)Stevioside and rebaudioside ASimultaneous analysis of water-solublevitaminsAscorbic acid and erythorbic acid1Sample : 0.05% each, 20μL1. Stevioside2. Rebaudioside A2Sample : 20μL1. Vitamin B 6 50μg/mL2. Nicotinamide 10μg/mL3. Vitamin B 12 10μg/mL4. Nicotinic acid 10μg/mL5. Folic acid 10μg/mL6. Vitamin C 10μg/mL41. Erythorbic acidO=CHOCOHOCHCHCOHCH 2 OHSample : 5μg/mL each, 10μL12. L-Ascorbic acidO=CHOCOHOCHC2HOCHCH 2 OH261350 5 10 150 1 2 3 4 5minmin0 5 10 minColumn : Shodex Asahipak NH2P-50 4EEluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minDetector : UV(210nm)Column temp. : 30˚CColumn : Shodex Asahipak NH2P-50 4EEluent : 40mM H 3 PO 4 aq./CH 3 CN=45/55Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 40˚CColumn : Shodex Asahipak NH2P-50 4EEluent : 20mM NaH 2 PO 4 + 30mM H 3 PO 4 aq./CH 3 CN=20/80Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 30˚C13

Standard AnalysisColumnsColumns for Silica-based Reversed Phase,HILIC and Normal Phase Chromatography[ODS columns]FeaturesFThis ODS column has been sold for over 30 years with reliable qualityC18M Monomeric type ODS column, fully end capped high purity silica (99.99% or higher)PreparativeColumnsp.79Columns for Silica-based Reversed Phase, HILIC and Normal Phase ChromatographyC18PF6604112F6605110F6604113F6650040F6650041F6650042F6650045F6650046F6650047E-4115SILODSpak F-411ODSpak F-511ODSpak F-411/SSilica C18M 4DSilica C18M 4ESilica C18M 2DSilica C18P 4DSilica C18P 4ESilica C18P 2D≥ 8,000≥ 14,000≥ 10,000≥ 10,000≥ 16,000≥ 9,000≥ 10,000≥ 16,000≥ 9,000[Other silica-based columns]5C85C45CN5NPE,5PYE5NHFeaturesPolymeric type ODS column, fully end capped high purity silica (99.99% or higher)Excellent acid toleranceAdvantageous for separating planar and nonplanar compounds from each otherSee also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction andnormal phase chromatography".Standard columnsProduct CodeProduct NamePlate Number(TP/column)FunctionalGroupOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylOctadecylParticle Size(µm)553555555Carbon Load(%)Silica gel packed column without any chemical modification141414161616171717Pore Size(Å)Uses high purity silica (99.99% or higher)Like E-411, suitable for normal phase analysis using a nonpolar organic solvent100100100100100100100100100Column Size (mm)I.D. x Length4.6 × 1504.6 × 2504.6 × 1004.6 × 1504.6 × 2502.0 × 1504.6 × 1504.6 × 2502.0 × 150Use when the retention capacity of C18 is too strong or that of 5C4 is too weekApplicable to ion pair chromatography, because of its rapid mass transfer and equilibrationUse when the retention capacity of C18 or C8 is too strongShipping SolventH 2 O/CH 3 OH=20/80H 2 O/CH 3 OH=20/80H 2 O/CH 3 OH=20/80H 2 O/CH 3 OH=30/70H 2 O/CH 3 OH=30/70H 2 O/CH 3 OH=40/60H 2 O/CH 3 OH=30/70H 2 O/CH 3 OH=30/70H 2 O/CH 3 OH=40/60Base Material : SilicaPreparativeColumnsPreparativeColumnsPreparativeColumnsUtilizes reversed phase interaction and π-electron interaction to separate regioisomers, which typicallycannot be separated with ODS, C8, or C4 columnsUtilizes several types of interactions based on π-electrons to separate structural isomersSuitable for saccharides analysis by hydrophilic interaction chromatography (HILIC)PreparativeColumnsp.79p.79p.79p.79See also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction andnormal phase chromatography".Standard columnsProduct CodeProduct NamePlate Number(TP/column)FunctionalGroupParticle Size(µm)Carbon Load(%)Pore Size(Å)Column Size (mm)I.D. x LengthShipping Solvent14F6604110F6650050F6650051F6650052F6650053F6650054F6650055F6650058F6650059F6650062F6650063F6650060F6650061Silicapak E-411Silica 5SIL 4DSilica 5SIL 4ESilica 5C8 4DSilica 5C8 4ESilica 5C4 4DSilica 5C4 4ESilica 5CN 4DSilica 5CN 4ESilica 5NPE 4DSilica 5PYE 4DSilica 5NH 4DSilica 5NH 4E≥ 8,000≥ 9,000≥ 15,000≥ 9,000≥ 15,000≥ 9,000≥ 15,000≥ 7,000≥ 12,000≥ 8,000≥ 7,000≥ 5,000≥ 8,000−−−OctylOctylButylButylCyanopropylCyanopropylNitrophenylethylPyrenylethylAminopropylAminopropyl5555555555555−−−101077−−−−−−501001001001001001001001001001001001004.6 × 1504.6 × 1504.6 × 2504.6 × 1504.6 × 2504.6 × 1504.6 × 2504.6 × 1504.6 × 2504.6 × 1504.6 × 1504.6 × 1504.6 × 250IPA/Dichloromethane/n-Hexane=1/50/49C 6 H 14 /C 2 H 5 OH=95/5C 6 H 14 /C 2 H 5 OH=95/5H 2 O/CH 3 OH=34/66H 2 O/CH 3 OH=34/66H 2 O/CH 3 OH=45/55H 2 O/CH 3 OH=45/55H 2 O/CH 3 OH=60/40H 2 O/CH 3 OH=60/40H 2 O/CH 3 OH=45/55H 2 O/CH 3 OH=30/70H 2 O/CH 3 CN=5/95H 2 O/CH 3 CN=5/95*Contact Shodex or our distributors near you for customized columns.Base Material : Silica

Hippuric acids in urineStandards2Sample : 10μL1. Mandelic acid 17μg/mL2. Hippuric acid 17μg/mL3. o-Methylhippuric acid 17μg/mL4. p-Methylhippuric acid 17μg/mL5. m-Methylhippuric acid 17μg/mL6. Creatinine 33μg/mL5Urine(10-fold diluted)AflatoxinsSample :5μg/L each, 20μL1. Derived Aflatoxin G 12. Derived Aflatoxin B 13. Aflatoxin G 24. Aflatoxin B 223Reference :"Test Methods Related toTotal Aflatoxin" in NoticeShoku-An No. 0816-1(August 16, 2011, Japan)4TFA methodStandard AnalysisColumns3461126005 10 min0510 min0 5 10 15 20 minColumn : Shodex ODSpak F-411Eluent : [20mM HColumn : Shodex Silica C18M 4E3 PO 4 + 20mM β-Cyclodextrin(pH2.5)]/CH 3 CN=88/12+ 4.5mM Sodium dodecyl sulfateEluent : H 2 O/CH 3 CN/CH 3 OH=60/10/30Flow rate : 1.0mL/minFlow rate : 1.0mL/minDetector : UV(225nm)Detector : Fluorescence(Ex. : 365nm, Em. : 450nm)Column temp. : 50˚CColumn temp. : 40˚CTrichothecene mycotoxinsSample : 20μL1. Nivalenol (INIV)2. Deoxynivalenol (DON)15 10 15 minColumn : Shodex Silica C18M 4EEluent : H 2 O/CH 3 CN/CH 3 OH=90/5/5Flow rate : 1.0mL/minDetector : UV(220nm)Column temp. : 40˚CBenzylpyridine isomers* 1 ppm each of DON andNIV were added to wheat sampleReference :Shoku-An No. 0717001(July 17, 2003, Japan)Test method of deoxynivalenol2Coenzyme Q100Sample : 5μL1. Coenzyme Q10 1mg/mL15 10 15minColumn : Shodex Silica C18P 4DEluent : CH 3 OH/C 2 H 5 OH=13/7Flow rate : 1.2mL/minDetector : UV(275nm)Column temp. : 35˚CDinitronaphthalene isomersCatechins in green tea123Sample :1. (-)-Epigallocatechin 1.0μg2. (-)-Catechin 1.0μg3 (-)-Epigallocatechin gallate 0.5μg4. (-)-Catechin gallate 1.0μg0 10 20 30 40 minColumn : Shodex Silica C18P 4DEluent : 0.1% H 3 PO 4 aq./CH 3 OH=20/80Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 30˚CSimultaneous analysis of vitamin E homologs4Columns for Silica-based Reversed Phase, HILIC and Normal Phase ChromatographySample :1. 2-Benzylpyridine2. 3-Benzylpyridine3. 4-Benzylpyridine1Sample :1. Naphthalene2. 1,5-Dimethylnaphthalene3. 1,5-Dinitronaphthalene4. 1,8-DinitronaphthaleneSample : 20μL1. α-Tocopherol 5μg/mL2. α-Tocotrienol 10μg/mL3. β-Tocopherol 5μg/mL4. γ-Tocopherol 5μg/mL5. γ-Tocotrienol 10μg/mL6. δ-Tocopherol 5μg/mL7. δ-Tocotrienol 10μg/mL61221234334570 5 10 15 20 25minColumn : Shodex Silica 5PYE 4DEluent : 20mM KH 2 PO 4 aq./CH 3 OH=40/60Flow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 30˚C0 5 10 15min051015minColumn : Shodex Silica 5SIL 4DColumn : Shodex Silica 5NPE 4DEluent : n-Hexane/Isopropanol/Acetic acidEluent : H 2 O/CH 3 OH=30/70=1000/6/5Flow rate : 1.0mL/minFlow rate : 1.0mL/minDetector : UV(254nm)Detector : Fluorescence(Ex. : 298nm, Em. : 325nm)Column temp. : 30˚CColumn temp. : 30˚C15

Standard AnalysisColumnsColumns for Ligand Exchange Chromatography* A list of elution volume of saccharides for Shodex columns is available.Please refer to our website (www.shodex.de) or technical notebook (No.2 and 3).FeaturesSC1011, 1821,SP0810KS-801, 802Separates saccharides by combination of ligand exchange and size exclusion modesThree types of counter ions are available: Ca 2+ , Pb 2+ , and Na+[KS-801, 802]Only water is required for the analysis of neutral sugarsPreparativeNo.3 Newsp.80No.6, 40, 43ColumnsNotebookColumns for Ligand Exchange ChromatographyKS-803~807DC-613,SZ5532,SC1211MN-431,SC1011-7FLigand exchange and size exclusionF6378102F6378103F6700090F6378105F6700081F6378010F6378020F6378025F6378035F6378050F6378060F6700020F6378070F6700021NotebookNotebookStandard columnsProduct CodeMN-431 is for the analysis of mannitol in conformity with USPSC1011-7F is for the analysis of sugar alcohols and saccharides in conformity with EPCa-type ligand exchange chromatography columnsSUGAR SC1011SUGAR SC1821SUGAR SC-LGSUGAR SP0810SUGAR SP-GSUGAR KS-801SUGAR KS-802SUGAR KS-803SUGAR KS-804SUGAR KS-805SUGAR KS-806SUGAR KS-GSUGAR KS-807SUGAR KS-807GLigand exchange and HILICProduct CodeSuitable for separation of polysaccharides by size exclusion modeCan be used in tandem e.g., KS-802 and KS-801Only water is required for the analysis of neutral sugarsNo.3No.2NewsNo.8, 13Separates by combination of ligand exchange and HILIC modesDC-613 can analyze sugars without removing sodium salts in the sampleSZ5532 is recommended for the separation of disaccharides or trisaccharidesSC1211 is suitable for separation of sugar alcoholsProduct NameProduct NamePlate Number(TP/column)≥ 13,000≥ 13,000(guard column)≥ 11,000(guard column)≥ 17,000≥ 17,000≥ 17,000≥ 17,000≥ 9,000≥ 9,000(guard column)≥ 4,000(guard column)Plate Number(TP/column)Functional Group(Counter Ion)Sulfo (Ca 2 + )Sulfo (Ca 2 + )Sulfo (Ca 2 + )Sulfo (Pb 2 + )Sulfo (Pb 2 + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )1,00010,000−1,000−1,00010,00050,000400,0005,000,000(50,000,000)*−(200,000,000)*−6610710666717171030308.0 × 3008.0 × 3006.0 × 508.0 × 3006.0 × 508.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3006.0 × 508.0 × 3008.0 × 50H 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 OH 2 O( )* Estimated value Base Material : Styrene divinylbenzene copolymerFunctional Group(Counter Ion)Exclusion Limit(Pullulan)ParticleSize(µm)Pore Size(Å)ParticleSize(µm)Column Size(mm)I.D. x LengthColumn Size(mm)I.D. x Length[KS-803~806]F7001003F6700170F7001300F6700110F7001400F6700120RSpak DC-613RSpak DC-GSUGAR SZ5532SUGAR SZ-GSUGAR SC1211SUGAR SC-G≥ 5,500(guard column)≥ 5,500(guard column)≥ 5,500(guard column)Sulfo (Na + )Sulfo (Na + )Sulfo (Zn 2 + )Sulfo (Zn 2 + )Sulfo (Ca 2 + )Sulfo (Ca 2 + )61066610100−100−50−6.0 × 1504.6 × 106.0 × 1504.6 × 106.0 × 2504.6 × 10H 2 O/CH 3 CN=30/70H 2 O/CH 3 CN=30/70H 2 O/CH 3 CN=30/70H 2 O/CH 3 CN=30/70H 2 O/CH 3 CN=75/25H 2 O/CH 3 CN=75/25Base Material : Styrene divinylbenzene copolymerFor the United States Pharmacopeia (USP)Product Code Product NameFunctional GroupParticle Size Column Size (mm)(Counter Ion)(µm)I.D. x LengthShipping SolventF6379230 USPpak MN-431Sulfo (Ca 2 + )84.0 × 250H 2 OSee p.71 for USP (Ver.35) Column List.Base Material : Styrene divinylbenzene copolymerPreparativeColumns[DC-613]PreparativeColumnsp.80p.80Shipping SolventShipping SolventFor the European Pharmacopeia (EP)16Product CodeF6379300Product NameEP SC1011-7FFunctional Group(Counter Ion)Sulfo (Ca 2 + )Particle Size(µm)8Column Size (mm)I.D. x Length7.8 × 300Shipping SolventH 2 O*Contact Shodex or our distributors near you for customized columns.Base Material : Styrene divinylbenzene copolymer

Standard AnalysisColumnsColumns for Ligand Exchange ChromatographyAnomer separation of saccharidesAnomer separation of saccharides affects thechromatogram.When using a SUGAR column to analyze saccharides, theanalysis at high temperatures would suppress the influence ofanomer separation, resulting in proper chromatograms.29˚C70˚CGlucose Mannose Xylose Galactose Arabinose0 10 20 min 0 10 20 min 0 10 20 min 0 10 20 min 0 10 20 minGlucose Mannose Xylose Galactose Arabinose0 10 20 min 0 10 20 min 0 10 20 min 0 10 20 min 0 10 20 minColumn : Shodex SUGAR SC1011Eluent : H 2 OFlow rate : 0.7mL/minDetector : RIColumn temp. : 29˚C, 70˚CHydrolyzed starchDp-10Dp-5Dp-1Sample : 20μLHydrolyzed starch 1%Sample :0.5% each, 10μL1Calibration curves for KS-800 series using pullulan2Molecular Weight (Pullulan)Saccharides in wood (model)Sample : 5μL1. Cellobiose 1.0%2. Glucose 1.5%3. Xylose 0.5%4. Galactose 0.5%5. Arabinose 0.5%6. Mannose 1.0%10 710 610 510 410 310 246 810 12Elution volume (mL)KS-801KS-802KS-803KS-804KS-805KS-806Column : Shodex SUGAR KS-800 seriesEluent : H 2 ODetector : RIColumn temp. : 80˚CKetohexosesSample : 0.025% each, 10μL1. Sorbose2. Fructose3. Tagatose4. Psicose1263 4 5341015 20 min5 10 1520min0 5 10 1520minColumn : Shodex SUGAR KS-802 x 2Eluent : H 2 OFlow rate : 1.0mL/minDetector : RIColumn temp. : 80˚CColumn : Shodex SUGAR SP0810Eluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 85˚CColumn : Shodex SUGAR SP0810Eluent : H 2 OFlow rate : 1.0mL/minDetector : RIColumn temp. : 80˚COligosaccharides in soybeanSaccharides related to raffinosebiosynthesisAcesulfame K and sucraloseSample : 0.1% each, 20μL1. Verbascose2. Stachyose3. Raffinose4. Sucrose5. Pinitol5Sample : 20μL, 0.1% each1. Raffinose2. Sucrose3. Galactinol4. Galactose5. myo-Inositol1235Sample : 20μL1. Acesulfame K 0.1%2. Sucrose 0.5%3. Glucose 0.5%4. Unknown from Acesulfame K5. Fructose 0.5%6. Sucralose 0.1%2423435114610 15 20 25 30min0 5 10 min0 5 10 1520min18Column : Shodex SUGAR KS-802 + KS-801Eluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 85˚CColumn : Shodex SUGAR SC1011Eluent : H 2 OFlow rate : 1.0mL/minDetector : RIColumn temp. : 80˚CColumn : Shodex SUGAR SC1011Eluent : 10mM CaSO 4 aq.Flow rate : 0.6mL/minDetector : RIColumn temp. : 80˚C

Sucrose and turanose12Sample : 0.5% each, 10μL1. Fructose2. Glucose3. Sucrose4. Turanose5. LactoseMaltose and isomaltose1Sample : 0.5% each, 20μL1. Glucose2. Maltose3. Isomaltose4. Maltotriose5. Isomaltotriose4Saccharides and sugar alcoholsSample : 0.5% each, 20μL1. Rhamnose2. Xylose3. Arabinose4. Fructose5. Glucose6. Galactose7. Arabitol8. Xylitol9. Mannitol10. SorbitolStandard AnalysisColumns3450 5 10 15 20 25 30 350 5 10 15 20 minminColumn : Shodex SUGAR SZ5532Eluent : H 2 O/CH 3 CN=20/80Flow rate : 0.6mL/minDetector : RIColumn temp. : 60˚CSaccharides in presence of sodium salt23Column : Shodex SUGAR SZ5532Eluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 60˚CSince DC-613 uses Na + as counter ion, it enables the analysis of saccharides insodium salt containing samples without desalting. Acid hydrolyzed sample canbe analyzed after neutralizing the remaining acid, such as hydrochloric or sulfuricacid, with sodium hydroxide.Solvent for samplepreparationSample : 0.1% each, 10μL1. Xylose2. Sucrose3. Cellobiose4. Lactose5Pinitol1 234 567 8 90 5 10 15 minColumn : Shodex SUGAR SZ5532Eluent : H 2 O/CH 3 CN=20/80Flow rate : 1.0mL/minDetector : RIColumn temp. : 65˚C10Sample : 0.1% each, 10μL1. Pinitol2. Fructose3. Glucose4. Sucrose5. chiro-Inositol6. myo-InositolColumns for Ligand Exchange Chromatography1% NaCl aq./CH 3 CN=50/50123411% Na 2 SO 4 aq./CH 3 CN=50/502345 6H 2 O/CH 3 CN=50/500 5 10 15min0510 1520minColumn : Shodex RSpak DC-613Eluent : H 2 O/CH 3 CN=30/70Flow rate : 0.8mL/minDetector : RIColumn temp. : 50˚C* High concentration of acetonitrile isused for saccharide analysis by DC-613.Pay attention to possible salt depositionwhen analyzing with high saltconcentration samples.Column : Shodex RSpak DC-613Eluent : H 2 O/CH 3 CN=25/75Flow rate : 1.0mL/minDetector : RIColumn temp. : 70˚CMoisturizing componentsMannitol analysis with USP methodSample : 0.1% each, 20μL1. 1,3-Propanediol2. Propylene glycol3. Ethylene glycol4. Glycerin5. meso-Erythritol1According to the USP (United States Pharmacopeia) method, mannitolshould be analyzed using a column which can separate mannitol andsorbitol with a resolution equal to or greater than 2.0.MN-431 is a column specially designed for mannitol analysis, whichsatisfies this criteria.14Sample : 0.5% each, 10μL1. Mannitol2. Sorbitol22350 5 10 15minColumn : Shodex SUGAR SC1211Eluent : H 2 O/CH 3 CN=60/40Flow rate : 0.6mL/minDetector : RIColumn temp. : 40˚C0 2Column : Shodex USPpak MN-431Eluent : H 2 OFlow rate : 0.5mL/minDetector : RIColumn temp. : 60˚C4 6 8min19

Standard AnalysisColumnsColumns for Ion Exclusion ChromatographyFeaturesSH1011, 1821 Columns for simultaneous analysis of saccharides and organic acidsSeparates neutral sugars in size exclusion mode and organic acids in ion exclusion modeSuitable for the analysis of uronic and aldonic acidsNotebook No.3 News No.25, 40, 43Columns for Ion Exclusion ChromatographyKC-811Product CodeF6378100NotebookStandard columnsColumns for the analysis of organic acidsIon exclusion mode (+ reversed phase mode)Highly selective detection with post column methodKC-811 6E is suitable for the analysis of cyanide ions and cyanogen chloride inaccordance with the Japanese Water Supply ActNo.3Product NameSUGAR SH1011NewsNo.6For simultaneous analysis of saccharides and organic acidsPlate Number(TP/column)≥ 17,000FunctionalGroupSulfoExclusion Limit(Pullulan)1,000ParticleSize(µm)6Column Size(mm)I.D. x Length8.0 × 300PreparativeColumnsShipping SolventH 2 Op.80F6378101SUGAR SH1821≥ 17,000Sulfo10,00068.0 × 300H 2 OF6700080SUGAR SH-G(guard column)Sulfo−106.0 × 50H 2 OBase Material : Styrene divinylbenzene copolymerFor organic acids, cyanide ions and cyanogen chlorideProduct CodeProduct NamePlate Number(TP/column)FunctionalGroupParticle Size(µm)Column Size(mm)I.D. x LenngthShipping SolventF6378030RSpak KC-811≥ 17,000Sulfo68.0 × 300H 2 OF6378033RSpak KC-811 6E≥ 13,000Sulfo66.0 × 250H 2 OF6700030RSpak KC-G(guard column)Sulfo106.0 × 50H 2 OF6700010RSpak KC-LG(guard column)Sulfo138.0 × 50H 2 O* As a guard column, use KC-LG for samples with relatively high impurityand KC-G for samples with relatively low impurity.Base Material : Styrene divinylbenzene copolymer20*Contact Shodex or our distributors near you for customized columns.

Maltooligosaccharides, organic acidsand ethanolSample : 0.05% each, 20μL1. Maltotetraose2. Maltotriose3. Maltose4. Glucose5. Lactic acid6. Glycerol7. Acetic acid8. Methanol9. Ethanol0 5 10 15 20 25 0min12 3Eluent = 2mM HClO 4 aq.42052512 345Column : Shodex SUGAR SH1821Eluent : 0.5mM H 2 SO 4 aq.Flow rate : 0.6mL/minDetector : RIColumn temp. : 75˚CHydrophobic organic acids6767830 35 40 min89Sample :1. Succinic acid2. Lactic acid3. Formic acid4. Acetic acid5. Propionic acid6. Isobutyric acid7. n-Butyric acid8. Isovaleric acid9. n-Valeric acidTo shorten analysis time9Cello-oligosaccharides and furfurals1 2 345675 10 15 20 25 30 35 40 45min4Sample : 0.1% each, 10μL1. Cellopentaose2. Cellotetraose3. Cellotriose4. Cellobiose5. Glucose6. Glyceric acid7. Acetic acid8. 5-HMF9. FurfuralColumn : Shodex SUGAR SH1821Eluent : 2mM H 2 SO 4 aq.Flow rate : 0.6mL/minDetector : RIColumn temp. : 60˚COrganic acids and vitamin C in fruit juice589Sample :1. Cl - ,etc.2. Oxalic acid3. Maleic acid4. Citric acid5. Malic acid6. Vitamin C7. Succinic acid8. Formic acid9. Acetic acidGeneral organic acidsSample :1. Citric acid2. Tartaric acid3. Pyruvic acid4. Malic acid5. Succinic acid6. Glycolic acid7. Lactic acid8. Fumaric acid9. Acetic acid10. Levulinic acid11. Pyroglutamic acid12. Propionic acid13. Isobutyric acid14. n-Butyric acid1012 4132356479810111213 1420 30minColumn : Shodex RSpak KC-811 x 2Eluent : 6mM HClO 4 aq.Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 50˚CGlucronolactone and organic acidsSample : 20μL1. Citric acid 0.01%2. Malic acid 0.01%3. Glucronolactone 0.01%4. Glycerin 0.01%5. Ethanol 0.05%5Standard AnalysisColumnsColumns for Ion Exclusion ChromatographyEluent = 2mM HClO 4 aq./CH 3 CN=90/10321678915202530 minColumn : Shodex RSpak KC-LG + KC-811 x 2Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 47˚C5101520 minColumn : Shodex RSpak KC-LG + KC-811 x 2Eluent : 1mM HClO 4 aq.Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 40˚C0 5 10 15minColumn : Shodex RSpak KC-811Eluent : 3mM HClO 4 aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚COrganic acids in sakeCyanide ion and cyanogen chloride withpost column methodSample : 100μL1. Phosphoric acid etc.2. Citric acid3. Pyruvic acid4. Malic acid5. Succinic acid6. Lactic acid7. Fumaric acid8. Acetic acid9. Pyroglutamic acid56Fine rice wine56Fermented liquorPure rice wine546CN -Sample : 10μg/L each, 100μLCNCl141142870 5 10 15 20 25min3Column : Shodex RSpak KC-LG + KC-811 x 2Eluent : 4.8mM HClO 4 aq.Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 63˚C9238 9723870 5 10 15 20 25 0 5 10 15 20min925min2 4 6 8 10 min 2 4 6 8 10 minColumnEluentReagent AReagent BFlow rate: Shodex RSpak KC-811 6E: 1.0mM H 2 SO 4 aq.: Chloramine T solution: 4-Pyridinecarboxylic acid-Pyrazolonesolution: (Eluent) 1.0mL/min(Reagent) 0.5mL/min each: VIS(638nm)DetectorColumn temp. : 40˚CReaction temp. : (Reagent A) 40˚C(Reagent B) 80˚C21

Standard AnalysisColumnsColumns for Ion Chromatography (Anion Analysis)Columns for Ion Chromatography (Anion Analysis)FeaturesNI-424, I-524ASI-90, SI-50NEW SI-35SI-52Standard columnsFor anions (non-suppressor method)ProductCodeF6995243F6709616F6995240F6700400Product NameIC NI-424IC NI-GIC I-524AIC IA-GColumns for anion analysis with non-suppressor methodNI-424 supports simultaneous analysis of fluoride and phosphate ionsColumns for anion analysis with suppressor methodSuitable for the quantitative analysis of fluoride ionSI-50 separates target inorganic anions from organic acidsNot interfered by the system peak derived from carbonateColumns for the analysis of oxyhalidesSI-35 supports rapid analysis of oxyhalides and general inorganic ionsSI-52 supports simultaneous analysis of oxyhalides and general inorganic ionsPlate Number(TP/column)≥ 5,000(guard column)Functional GroupQuaternary ammoniumQuaternary ammonium≥ 2,000 Quaternary ammonium(guard column) Quaternary ammoniumParticle Size(µm)551212Column Size(mm)Shipping SolventI.D. x Length8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris4.6 × 100+ 2mM Phenylboronic acid + 0.005mM CyDTA aq.8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris4.6 × 10+ 2mM Phenylboronic acid + 0.005mM CyDTA aq.4.6 × 1004.6 × 102.5mM Phthalic acid aq.2.5mM Phthalic acid aq.Base Material : PolyhydroxymethacrylateFor anions (suppressor method)ProductCodeF6995244Product NameIC SI-90 4EPlate Number(TP/column)≥ 5,000Functional GroupQuaternary ammoniumParticle Size(µm)9Column Size(mm)I.D. x Length4.0 × 250Shipping Solvent1.8mM Na 2 CO 3 + 1.7mM NaHCO 3 aq.F6709620IC SI-90G(guard column)Quaternary ammonium94.6 × 101.8mM Na 2 CO 3 + 1.7mM NaHCO 3 aq.F6995245IC SI-50 4E≥ 10,000Quaternary ammonium54.0 × 2503.2mM Na 2 CO 3 + 1.0mM NaHCO 3 aq.F6709625IC SI-50G(guard column)Quaternary ammonium54.6 × 103.2mM Na 2 CO 3 + 1.0mM NaHCO 3 aq.For oxyhalides (suppressor method)Base Material : Polyvinyl alcoholHousing Material : PEEKProductCodeProduct NamePlate Number(TP/column)Functional GroupParticle Size(µm)Column Size(mm)I.D. x LengthShipping SolventF6995290NEWIC SI-35 4D≥ 13,000Quaternary ammonium3.54.0 × 1503.6mM Na 2 CO 3 aq.F6709627NEWIC SI-95G(guard column)Quaternary ammonium94.6 × 103.6mM Na 2 CO 3 aq.F6995260IC SI-52 4E≥ 14,000Quaternary ammonium54.0 × 2503.6mM Na 2 CO 3 aq.F6709626IC SI-92G(guard column)Quaternary ammonium94.6 × 103.6mM Na 2 CO 3 aq.Base Material : Polyvinyl alcoholHousing Material : PEEKLine filters for ICProductCodeProduct NameApplicationContentsF8500630IC FL-1For general purposesOne holder and one filterF8500640IC FL-1 filterReplacement filter for IC FL-15 filtersF8500650IC FL-2Non-metal typeOne holder and one filterF8500660IC FL-2 filterReplacement filter for IC FL-24 filters22*Contact Shodex or our distributors near you for customized columns.

Anions analysis with non-suppressor method (NI-424 and I-524A)12345NI-42467Sample : 20μL1. H 2 PO - 4 10mg/L2. F - 1mg/L3. CI - 1mg/L4. NO - 2 5mg/L5. Br - 5mg/L6. NO - 3 5mg/L7. SO 2- 4 5mg/L1,234567I-524ANI-424 is a high performance typecolumn and offers twice increasedtheoretical plate number than I-524A.(1) Enables the separation of H 2 PO 4-and F - ions which were difficult toseparate with I-524A.(2) The shape of each peak is sharper,and the separation balance isproper. Especially, the separationof Cl - and NO 2- is improved.Standard AnalysisColumns0125 10 15 min 0 5 10 15 minColumn : Shodex IC NI-424Column : Shodex IC I-524AEluent : 8mM 4-Hydroxybenzoic acid+ 2.8mM Bis-Tris + 2mM Phenylboronic acidEluent : 2.5mM Phthalic acid+ 2.3mM Tris(hydroxymethyl)aminomethane aq.+ 0.005mM *CyDTA aq.Flow rate : 1.2mL/minFlow rate : 1.0mL/minDetector : Non-suppressed conductivityDetector : Non-suppressed conductivityColumn temp. : 40˚CColumn temp. : 40˚C*CyDTA : trans-1,2-Diaminocyclohexane-N,N,N',N'-tetra acetic acidAnions analysis using SI-90 4E (suppressor method)34578Sample : 20μL1. F - 2mg/L2. Cl - 3mg/L3. NO - 2 5mg/L4. Br - 10mg/L5. NO - 3 10mg/L6. HCO - 3 300mg/L7. PO 3- 4 15mg/L8. SO 2- 4 15mg/LPerchloric acid analysis using SI-90 4E (suppressor method)123Sample : 100μL1. Cl - 3mg/L2. NO - 3 10mg/L3. SO 2- 4 15mg/L4. ClO - 4 10mg/LColumns for Ion Chromatography (Anion Analysis)460 5 10 15minColumn : Shodex IC SI-90 4EEluent : 1.8mM Na 2 CO 3 + 1.7mM NaHCO 3 aq.Flow rate : 1.5mL/minDetector : Suppressed conductivityColumn temp. : Room temp.(25˚C)0 5 10 15minColumn : Shodex IC SI-90 4EEluent : 6mM Na 2 CO 3 aq. + 10% CH 3 CNFlow rate : 1.0mL/minDetector : Suppressed conductivityColumn temp. : 25˚CAnions analysis using SI-50 4E (suppressor method)SI-50 4E is a high performance type of SI-90 4E.Acetic acid, formic acid, and methacrylic acid elutedbetween F - and Cl - .The carbonate system peak appears between NO 2-and Br - peaks.15678910Sample : 20μL1. F - 2mg/L2. Acetic acid 10mg/L3. Formic acid 2mg/L4. Methacrylic acid 10mg/L5. Cl - 3mg/L6. NO 2- 5mg/L7. Br - 10mg/L8. NO 3- 10mg/L9. PO 43- 15mg/L10. SO 42- 15mg/L11. Oxalic acid 15mg/L11Oxyhalides and anions analysis using SI-52 4E (suppressor method)SI-52 4E is a high resolution type column and offers 14,000 or highertheoretical plate numbers. It supports simultaneous analysis of oxyhalidesand inorganic anions. The recommended analysis temperature is 45˚C.14911Sample : 50μL1. F - 2mg/L2. ClO - 2 1mg/L3. BrO - 3 1mg/L4. Cl - 10mg/L5. NO - 2 5mg/L6. Br - 10mg/L7. ClO - 3 1mg/L8. Dichloroacetic acid1mg/L9. NO - 3 30mg/L10. PO 3- 4 15mg/L11. SO 2- 4 40mg/L2 345610237 805 10 15 20 25 30 minColumn : Shodex IC SI-50 4EEluent : 3.2mM Na 2 CO 3 + 1.0mM NaHCO 3 aq.Flow rate : 0.7mL/minDetector : Suppressed conductivityColumn temp. : 25˚C0 5 10 15 20 25 30 minColumn : Shodex IC SI-52 4EEluent : 3.6mM Na 2 CO 3 aq.Flow rate : 0.8mL/minDetector : Suppressed conductivityColumn temp. : 45˚C23

Standard AnalysisColumnsColumns for Ion Chromatography (Cation Analysis)FeaturesYS-50 High performance type of YK-421Applicable to both suppressor and non-suppressor methodsPeak shape is sharper, especially for divalent cation analysisSupports the analysis of alkylamines and transition metalsColumns for Ion Chromatography (Cation Analysis)YK-421Y-521T-521Standard columnsFor cationsProductCodeF7122000F6700530Column for cation analysis with non-suppressor methodSimultaneous analysis of monovalent and divalent cationsSuitable for separation of alkylaminesColumn for cation analysis with non-suppressor methodFor the separation of monovalent or divalent cationsColumn for transition metal ion analysisHigh sensitivity analysis is achieved by post column color reaction methodProductNameIC YS-50IC YS-GPlate Number(TP/column)≥ 5,500(guard column)FunctionalGroupCarboxylCarboxylBase MaterialPolyvinyl alcoholPolyvinyl alcoholParticleSize(µm)55Column Size(mm)I.D. x Length4.6 × 1254.6 × 10Shipping SolventH 2 OH 2 OF7120012IC YK-421≥ 2,800CarboxylSilica54.6 × 1255mM Tartaric acid + 1mM Dipicolinicacid + 1.5g/L Boric acid aq.F6709608IC YK-G(guard column)CarboxylSilica54.6 × 105mM Tartaric acid + 1mM Dipicolinicacid + 1.5g/L Boric acid aq.F6995210IC Y-521≥ 3,000SulfoStyrene divinylbenzene copolymer124.6 × 1504mM HNO 3 aq.F6700230IC Y-G(guard column)SulfoStyrene divinylbenzene copolymer124.6 × 104mM HNO 3 aq.For transition metal ionsProductCodeProductNamePlate Number(TP/column)FunctionalGroupBase MaterialParticleSize(µm)Column Size(mm)I.D. x LengthShipping SolventF6995250IC T-521≥ 3,000SulfoStyrene divinylbenzene copolymer124.6 × 1503mM HNO 3 aq.F6700412IC T-G(guard column)SulfoStyrene divinylbenzene copolymer124.6 × 103mM HNO 3 aq.Housing Material : PEEKLine filters for ICProductCodeProduct NameApplicationContentsF8500630IC FL-1For general purposesOne holder and one filterF8500640IC FL-1 filterReplacement filter for IC FL-15 filtersF8500650IC FL-2Non-metal typeOne holder and one filterF8500660IC FL-2 filterReplacement filter for IC FL-24 filters24*Contact Shodex or our distributors near you for customized columns.

Standard cations (YS-50 and YK-421)1234YS-5010μL inj.56Sample :1. Li + 2mg/L2. Na + 10mg/L3. NH + 4 10mg/L4. K + 20mg/L5. Mg 2+ 10mg/L6. Ca 2+ 20mg/LYK-421213415μL inj.65YS-50 is a high performance typecolumn offers twice increasedtheoretical plate number than YK-421does. In particular, the peak shape isimproved. The quantitative performancefor NH 4+ in a sample containing highconcentration Na + is also enhanced.Resolutionfactor ofNa + and NH 4+YS-502.5YK-4212.1Standard AnalysisColumns018-Crown 6-ether conc.6mM3mM1mM0mM5 10 15 20 min 0 5 10 15 20 minColumn : Shodex IC YS-50Eluent : 4mM Methanesulfonic acid aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚CEffects of crown ether in eluentCrown ether forms complex with cations. The elution ofcations (particularly K + ) can be well controlled by thedifference in complex formation rate.1221 321 33213444545556666Sample : 10μL1. Li + 2mg/L2. Na + 10mg/L3. NH + 4 10mg/L4. K + 20mg/L5. Mg 2+ 10mg/L6. Ca 2+ 20mg/L1 234560 5 10 15 20 25 30 35minTPMg 2+Ca 2+Column : Shodex IC YK-421Eluent : 5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚CYS-507YK-4216,900 3,0006,600 3,000Simultaneous analysis for cations and ethylenediamineSample : 10μL1. Li + 2mg/L2. Na + 10mg/L3. NH + 4 10mg/L4. K + 20mg/L5. Mg 2+ 10mg/L6. Ca 2+ 20mg/L7. Ethylenediamine 50mg/LColumns for Ion Chromatography (Cation Analysis)05 10 15 20 minColumn : Shodex IC YS-50Eluent : 4mM Methanesulfonic acid + 18-Crown 6-ether aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚CColumn : Shodex IC YS-50Eluent : 4mM Nitric acid + 1.5mM 18-Crown 6-ether aq./CH 3 CN=90/10Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚CAlkylaminesAlkaline earth metal ions1Sample : 50μL1. NH + 4 5mg/L2. Methylamine 5mg/L3. Dimethylamine 5mg/L4. Trimethylamine 20mg/L5. Ethylamine 10mg/L6. Propylamine 10mg/L7. Butylamine 10mg/Lmin2Cu 2+Zn 2+Ni 2+Co 2+3Mg 2+Fe 2+ , Fe 3+Mn 2+ , Pb 3+Cd 2+4Ca 2+13Sample : 10μL1. Ni 2+ 50mg/L2. Mg 2+ 20mg/L3. Mn 2+ 50mg/L4. Ca 2+ 50mg/L42345Sr 2+2256767Ba 2+0 1 2 3 4 5 min05 10 minColumn : Shodex IC YK-421Eluent : 4mM H 3 PO 4 aq./CH 3 CN=90/10Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 25˚C8Column : Shodex IC Y-521Eluent : 4mM Tartaric acid + 2mM Ethylenediamine aq.Flow rate : 1.0mL/minDetector : Non-suppressed conductivityColumn temp. : 40˚C25

Standard AnalysisColumnsColumn Selection for Size ExclusionChromatography (SEC)Application Solvent ColumnPageBiological macromolecules(proteins, peptides, nucleic acids, etc.)Buffer etc.Buffer etc.KW-800 seriesKW400 seriesHigh performance(solvent-saving)2828Column Selection for Size Exclusion Chromatography (SEC)Aqueous SEC(GFC)Organic SEC(GPC)Biological macromolecules(high MW range)Water-soluble polymers(polyacrylamide, polyethylenimine, etc.)PolysaccharidesOligosaccharide, polysaccharidesGeneral polymersPolar polymers(polyimides, polyvinylpyrrolidones etc.)Analysis at high temperature(polyethylene, polypropylene etc.)Buffer etc.H 2 O, BufferAqueoussolution etc.H 2 O,AqueoussolutionTHFTHFTHFTHFChloroformDMFDMFODCB etc.ODCB etc.ODCB etc.SB-800 HQ seriesSB-800 HQ seriesKS-800 seriesGS-HQ seriesKF-800 seriesKF-600 seriesKF-400HQ seriesLF seriesK-800 seriesKD-800 seriesSB-800 HQ seriesHT-800 seriesUT-800 seriesAT-806MSRapid analysis(solvent-saving)High performance(solvent-saving)High linearity ofcalibration curve3030163236424244384030464646Engineering resin analysis at roomtemperature[polyamide (Nylon), polyethyleneterephthalate (PET) etc.]HFIPHFIPHFIP-800 seriesHFIP-600 seriesRapid analysis(solvent-saving)High linearity ofHFIP LF series 44calibration curve4848Aqueous/OrganicSECGF-HQ series34HighGuideline for column solvent usabilityPolarity of solventLowH 2O Methanol DMSO Acetonitrile DMF THF Chloroform TolueneKW-800 series, KW400 seriesSB-800 HQ seriesGS-HQ seriesGF-HQ seriesKD series26KF, K, LF series* See page 50 for the solvent replaceability of organic solvent SEC (GPC) packed columns.

Precautions for Polar Polymer AnalysisSize exclusion chromatography analysis of polar polymers can be influenced by unexpected interactions in the column.These interactions may change elution patterns and results in an invalid molecular weight calculation.It is important to reduce them in order to obtain the accurate molecular weight distribution.Interfering interactions likely to be observedStandard AnalysisColumnsInteractions between the analyte and the packing materialsHydrophobic interactionThe analyte is adsorbed into the packing material.This delays the analyte elution, and thus results inunderestimation of its molecular weight (Figure B, D).Ionic interaction(1) Ion ExclusionThe analyte is repelled from the packing material.This accelerates the analyte elution, and thus resultsin overestimation of its molecular weight (Figure A, C).(2) Ion ExchangeThe analyte is adsorbed onto the packing material.This delays the analyte elution, and thus results inunderestimation of its molecular weight (Figure B, D).Interaction within and between the analyteIonic repulsion effects observed within the multivalentmacromolecules causes structure expansionThis accelerates the analyte elution, and thus results inover estimation of its molecular weight (Figure A).Association between the moleculesAssociated molecule detected as a larger molecule(Figure A).Exclusion limitmolecular weightlog MWElution will beacceleratedExclusionlimit volume(C)(A)Elution will beretarded(B)Permeationlimit volume(D)Precautions for Polar Polymer AnalysisInteractions between the analyte and the solventThe multivalent ion of the solvent works as a bridge tobind ionic molecules (analyte).Methods to reduce interactionsAqueous SEC (GFC)Organic solvent SEC (GPC)Ionic InteractionAdd saltHydrophobic interactionIncrease dissociation of the analyteCationic polymer Lower the pHAnionic polymer Higher the pHLower the eluent polarity(Example) Add acetonitrile or methanolIonic InteractionAdd salt(Example) Add LiBr to DMFAdd CF 3 COONa to HFIPHydrophobic interactionLower the polarity of the eluent(Example) Change the eluent from DMF to THFHydrophillic interactionIncrease the polarity of the eluent(Example) Change the eluent from THF to DMF27

Standard AnalysisColumnsAqueous SEC (GFC) Columns : Silica-basedFeaturesKW-800Silica-based packed columns for aqueous SEC (GFC) analysisSuitable for the analysis of proteins and enzymesNotebookNo.7PreparativeColumnsp.81KW400Reduced particle size of the packing material enhances column performanceAqueous SEC (GFC) Columns : Silica-basedThree- or fourfold higher sensitivity than KW-800 seriesKW405-4F is applicable to samples with a molecular weight above 1,000,000NotebookNo.5, 7Standard columnsProduct CodeF6989000F6989103F6989104F6700131Product NameNewsPROTEIN KW-802.5PROTEIN KW-803PROTEIN KW-804PROTEIN KW-GNo.31Plate Number(TP/column)≥ 21,000≥ 21,000≥ 16,000(guard column)Exclusion Limit(Pullulan) (Protein)60,000170,000500,000−150,000700,000(1,000,000)*−ParticleSize(µm)5577MaximumPore Size(Å)4001,0001,500−Column Size(mm)I.D. x Length8.0 × 3008.0 × 3008.0 × 3006.0 × 50Semi-microMicro ColumnsShippingSolventH 2 OH 2 OH 2 OH 2 Op.73Base Material : SilicaUsable pH range : pH3.0-7.5( )* Estimated valueHigh performance semi-micro columnsUse of the KW400 series with semi-micro type devices is recommended.Product CodeProduct NamePlate Number(TP/column)Exclusion Limit(Pullulan) (Protein)ParticleSize(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LengthShippingSolventF6989201KW402.5-4F≥ 35,00060,000150,00034004.6 × 300H 2 OF6989202KW403-4F≥ 35,000150,000600,00038004.6 × 300H 2 OF6989203KW404-4F≥ 25,000500,000(1,000,000)*51,5004.6 × 300H 2 OF6989204KW405-4F≥ 25,0001,300,000(20,000,000)*52,0004.6 × 300H 2 OF6700132KW400G-4A(guard column)−−5−4.6 × 10H 2 OBase Material : SilicaUsable pH range : pH3.0-7.5( )* Estimated valueMolecular weight range with protein (eluent : phosphate buffer)Molecular weight (Protein)10 2 10 3 10 4 10 5 10 6 10 7 10 8KW-802.5, 402.5-4FKW-803, 403-4FKW-804, 404-4FKW405-4F28CalibrationStandardsSee page 51 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Calibration curves for KW400 series and KW-800 series using proteinMolecular weight (Protein)10 7 KW404-4F10 6KW405-4F10 510 4KW402.5-4FKW403-4F10 310 2 1.5 2.0 2.5 3.0 3.5 4.0 4.5Elution volume (mL)Comparison of KW402.5-4F and KW-802.5KW400 series are high performance typesemi-micro columns, and offer approximately 1.5times larger theoretical plate number and 3 to 4times higher detection sensitivity (peak height) thanKW-800 series columns.Sample : 10μL51. Blue dextran 2000 0.2mg/mL2. γ-Globulin 0.8mg/mL3. Ovalbumin 0.8mg/mL4. Myogrobin 0.56mg/mL5. Uridine 0.04mg/mLN=17,000KW402.5-4F 1 2 34Molecular weight (Protein)10 610 510 410 3KW-802.5Column : Shodex KW400-4F series, PROTEIN KW-800 seriesEluent : 50mM Sodium phosphate buffer + 0.3M NaCl(pH7.0)Flow rate : (KW400) 0.33mL/min(KW-800) 1.0mL/minDetector : (KW400) UV(280nm) (small cell volume)(KW-800) UV(280nm) (conventional type)Column temp. : 25˚CKW-803KW-80410 25 6 7 8 9 10 11 12 13Elution volume (mL)Whey in yogurtSample : Whey, 5μLRecovery rate of proteinsγ - GlobulinLectinsProteinBovine serum albuminOvalbuminMyoglobinCytochrome cLysozymeα-Chymotrypsinogen ARecovery (%)KW402.5-4F KW403-4FColumn : Shodex KW402.5-4F, KW403-4FEluent : 50mM Sodium phosphate buffer+ 0.3M NaCl(pH7.0)Flow rate : 0.33mL/minDetector : UV(280nm) (small cell volume)Column temp. : 25˚CSample : 5μL1. Lectin from Soybean 0.6mg/mL2. Lectin from Arachis hypogaea 1.1mg/mL3. Lectin from Canavalia ensiformis (Con A) 0.9mg/mL4. Lectin from Lens culinaris (LCA) 0.7mg/mL5. Lectin from Triticum vulgaris (WGA) 0.8mg/mL21988989909287953 4 596969789929894Standard AnalysisColumnsAqueous SEC (GFC) Columns : Silica-based10 7 5 10KW-802.5N=8,2001 2 3 4505 1015minColumn : Shodex KW402.5-4FPROTEIN KW-802.5Eluent : 50mM Sodium phosphate buffer+ 0.3M NaCl(pH7.0)Flow rate : (A) 0.33mL/min, (B) 1.0mL/minDetector : UV(280nm) (small cell volume)Column temp. : 25˚C20 30 4050 min 0 5 10 minColumn : Shodex KW402.5-4F + KW403-4F Column : Shodex KW402.5-4FEluent : 50mM Sodium phosphate bufferEluent : 50mM Sodium phosphate buffer+ 0.3M NaCl(pH7.0)+ 0.3M NaCl(pH7.0)Flow rate : 0.20mL/minFlow rate : 0.33mL/minDetector : UV(280nm) (small cell volume)Detector : UV(220nm) (small cell volume)Column temp. : 30˚CColumn temp. : 30˚CComparison of various GFC columns for separation ofstandard proteinsSample :1. Thyroglobulin (bovine)2. γ-Globulin (bovine)3. Ovalbumin (chicken)4. Myoglobin (horse)5. Cyanocobalamin4132132SB-803 HQ1GF-510 HQKW-803423Molecular weight (Protein)0 5 10 15 20 25 30 35 min45Three aqueous SEC columns (SB-803 HQ, GF-510 HQ,and KW-803) were compared for their separationperformances. KW-803, silica-based column, showedthe best separation performance for the analysis ofprotein standards.5510 6 KW-803GF-510 HQSB-803 HQ10 510 411 13 15 17 19 21 minColumn : Shodex OHpak SB-803 HQAsahipak GF-510 HQPROTEIN KW-803Eluent : 0.2M Phosphate buffer(pH6.9)Flow rate : 0.5mL/minDetector : UV(280nm)Column temp. : 30˚CProteins in human blood serum123456780 15 minColumn : Shodex PROTEIN KW-803Eluent : 50mM Phosphate buffer + 0.3M NaCl(pH7.0)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Sample : 0.1% each1. Fibrinogen 50μL2. α 2 -Macroglobulin 50μL3. IgG 50μL4. Transferrin 50μL5. Plasminogen 50μL6. Albumin 100μL7. Antitrypsin 100μL8. Hemoglobin 100μL29

Standard AnalysisColumnsAqueous SEC (GFC) Columns : Polymer-basedAqueous SEC (GFC) Columns : Polymer-basedFeaturesSB-800 HQSB-807 HQF6429100F6429101F6429102F6429103F6429104F6429105F6429106F6709430Polymer-based packed columns for aqueous SEC (GFC) analysisSupports a wide range of molecular weight sample analysisThe eluent can be replaced with DMF (except SB-802 HQ and SB-807 HQ),enabling the analysis of polar polymersMethod using SB-804 HQ or SB-805 HQ for gelatin’s mean molecular weightdetermination is comparable with PAGI method (Ver. 10, Japan)NewsStandard columnsProduct CodeNo.29Column for the analysis of water-soluble ultra high molecular weight polymersLarge particle size gel is packed to prevent shear degradation of polymersProduct NameOHpak SB-802 HQOHpak SB-802.5 HQOHpak SB-803 HQOHpak SB-804 HQOHpak SB-805 HQOHpak SB-806 HQOHpak SB-806M HQOHpak SB-GPlate Number(TP/column)≥ 12,000≥ 16,000≥ 16,000≥ 16,000≥ 12,000≥ 12,000≥ 12,000(guard column)SB-806M HQ is a mixed-gel column capable of analyzingsamples over a wide range of molecular weight distribution.For water-soluble ultra high molecular weight polymersExclusion Limit(Pullulan)4,00010,000100,000(1,000,000)(4,000,000)*(20,000,000)*(20,000,000)*−Particle Size(µm)8661013131310MaximumPore Size(Å)Column Size(mm)I.D. x LengthSemi-microMicro ColumnsPreparativeColumnsp.74p.81Shipping Solvent1002008002,0007,00015,00015,000−8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3006.0 × 500.02% NaN 3 aq.0.02% NaN 3 aq.0.02% NaN 3 aq.0.02% NaN 3 aq.0.02% NaN 3 aq.0.02% NaN 3 aq.0.02% NaN 3 aq.0.02% NaN 3 aq.Base Material : PolyhydroxymethacrylateUsable pH range : pH3-10( )* Estimated valueProduct CodeProduct NamePlate Number(TP/column)Exclusion Limit(Pullulan)Particle Size(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LengthShipping SolventF6429108OHpak SB-807 HQ≥ 1,500(500,000,000)*3530,0008.0 × 300H 2 OF6709431OHpak SB-807G(guard column)−35−8.0 × 50H 2 OUsable concentration of organic solventsProduct NameSB-802 HQSB-802.5 HQ, SB-803 HQSB-804 HQ~SB-806M HQSB-GSB-807 HQ, SB-807GThe maximum usable concentration (%)Methanol Acetonitrile DMF010075753007575753001001001000(Note)The maximum solvent tolerance ofpreparative type SB-800 HQ, SB-2000series, is 50% of methanol, acetonitrile,and DMF (SB-2002 is not tolerant oforganic solvents, similar to SB-802 HQ).Base Material : PolyhydroxymethacrylateUsable pH range : pH3-10( )* Estimated valueCalibrationStandardsSee page 51 forCalibration StandardsMolecular weight range with pullulan(eluent : ultrapure water)Molecular weight (Pullulan)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9SB-802 HQSB-802.5 HQSB-803 HQSB-804 HQSB-805 HQSB-806 HQSB-807 HQSB-806M HQMolecular weight range with PEG/PEO(eluent : DMF)Molecular weight (PEG/PEO)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9SB-802.5 HQSB-803 HQSB-804 HQSB-805 HQSB-806 HQSB-806M HQ30*Contact Shodex or our distributors near you for customized columns.

Calibration curves for SB-800 HQ seriesusing pullulan (eluent:H 2 O)Molecular weight (Pullulan)10 710 610 510 410 310 24 5 6 7 8 9 10 11 12Elution volume (mL)Calibration curve of Sodium polystyrene sulfonateHUMIC ACID(Dando soil)SB-802 HQHUMIC ACID(Inogashira soil)SB-806 HQSB-805 HQSB-803 HQSB-802.5 HQSB-804 HQSB-806M HQColumn : Shodex OHpak SB-800 HQ seriesEluent : H 2 OFlow rate : 1.0mL/minDetector : RIColumn temp. : Room temp.SEC analysis of humic substanceCalibration curves for SB-800 HQ seriesusing PEG/PEO (eluent:DMF)Molecular weight (PEG/PEO)10 710 610 510 410 310 2 4 5 6 7 8 9 10 11 12Elution volume (mL)SB-803 HQSB-804 HQSB-802.5 HQSB-805 HQSB-806M HQSB-806 HQColumn : Shodex OHpak SB-800 HQ seriesEluent : 20mM LiBr in DMFFlow rate : 0.8mL/minDetector : RIColumn temp. : 40˚CReference materials (from the JapaneseHumic Substances Society) for humicacid and fulvic acid, prepared frombrown forest soil (Dando) and kurobokusoil (Inogashira), were analyzed usingSEC columns.Sample : Humic substances, 30μLHumic acid 0.02mg/mLFulvic acid 0.02mg/mLDextran sulfateMW : 5,000MW : 500,0005 10 15 20 25 30minSample : Polyallylamine 0.2%, 100μL0.2 MSample : Dextran sulfate 0.1%, 200μLColumn : Shodex OHpak SB-806M HQ x 2Eluent : 0.1M NaCl aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CEffects of sodium nitrate concentration in eluenton the analysis of polyallylamineFor the analysis of cationic polymers, such as polyallylamine,undesired adsorption of the polymer is observed when low(0.02M) sodium nitrate eluent was used. By using higherconcentration (>0.1M) salt, it suppresses the sample adsorptionand enables to obtain accurate chromatograms.0.1 M 0.02 MStandard AnalysisColumnsAqueous SEC (GFC) Columns : Polymer-basedFULVIC ACID(Dando soil)FULVIC ACID(Inogashira soil)0.05 M7 9 11 13 15 17 minVoColumn : Shodex OHpak SB-G + SB-805 HQEluent : 10mM NaH 2 PO 4 + 10mM Na 2 HPO 4 (pH7.0) + 25% CH 3 CNFlow rate : 0.8mL/minDetector : UV(260nm)Data courtesy of Associate Professor Nobuhide Fujitake,Column temp. : 40˚CStudent Affairs Section, Graduate School of Agricultural Science,Kobe UniversityVt8.012.0 16.0 20.0 24.0Volume (mL)Column : Shodex OHpak SB-806M HQ x 2Eluent : 0.5M Acetic acid + NaNO 3 aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CCarboxymethylcellulose Polycarbonate PolyacrylamideSample : 200μLCarboxymethylcellulose (Medium viscosity) 0.1%Sample : PolycarbonateSample : Polyacrylamide, 100μLCH3SB-806 HQOCCH 3O COn1.0x10 8SB-807 HQMolar Mass(g/mol)1.0x10 71.0x10 6 6.0 8.0 10.0 12.0 14.0Mw: 5,500,0001.0x10 54.0Volume(mL)02 4 6 8 10 12 14 16 18 20 22 24 26 28 30min8 12 16 20 minColumn : Shodex OHpak SB-806M HQ x 2Eluent : 0.1M NaCl aq.Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex OHpakSB-805 HQ + SB-802.5 HQEluent : 5mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex OHpak SB-807 HQ, SB-806 HQEluent : 0.2M NaCl aq.Flow rate : 0.5mL/minDetector : RIMALS(Multi angle laser light scattering)Column temp. : 30˚C31

Standard AnalysisColumnsMultimode ColumnsMultimode ColumnsGS-HQFeaturesGS-620 7G-PSEC is the main separation modeAccording to the eluent selected, the column adds multimode features ofreversed phase, HILIC, and ion exchange modes to SECSuitable for the separation of peptides or nucleic acids with similar molecularweightsSuitable for desalting samples or substituting buffer in protein analysisNotebookNo.3Columns to determine molecular weight distribution of gelatin compliant withPAGI method (Ver. 10, Japan)Semi-microMicro ColumnsPreparativeColumnsp.75p.82Standard columnsProduct CodeProduct NamePlate Number(TP/column)Exclusion Limit(Pullulan)ParticleSize(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LengthShipping SolventF7600005Asahipak GS-220 HQ≥ 19,0003,000*61507.5 × 300H 2 O/CH 3 OH=70/30F7600006Asahipak GS-320 HQ≥ 19,00040,00064007.5 × 300H 2 O/CH 3 OH=70/30F7600007Asahipak GS-520 HQ≥ 18,000300,00072,0007.5 × 300H 2 O/CH 3 OH=70/30F7600008Asahipak GS-620 HQ≥ 18,000(2,000,000)**77,0007.5 × 300H 2 O/CH 3 OH=70/30F6710019Asahipak GS-2G 7B(guard column)−9−7.5 × 50H 2 O/CH 3 OH=70/30Molecular weight range with pullulan (eluent : ultrapure water)Molecular weight (Pullulan)10 10 2 10 3 10 4 10 5 10 6 10 7Base Material : Polyvinyl alcoholUsable pH range : pH2-12(GS-220 HQ : pH2-9)Usable concentration of methanol is up to 100%(GS-220 HQ : up to 30%)Usable concentration of acetonitrile is up to 50%* PEG equivalent( )** Estimated valueGS-220 HQGS-320 HQGS-520 HQGS-620 HQFor determination of gelatin molecular weight distributionProduct CodeProduct NameApplicationColumn Size(mm)I.D. x LengthShipping SolventF7600023Asahipak GS-620 7G-PGelatin for photo film7.5 × 500H 2 O/CH 3 OH=70/30F6710019Asahipak GS-2G 7B(guard column)7.5 × 50H 2 O/CH 3 OH=70/3032CalibrationStandardsSee page 51 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Calibration curves for GS-HQ series usingpullulan and PEGMolecular weight10 510 410 310 2 4 5 6 7 8 9 10 11 1210 6 Elution volume (mL)GS-320 HQGS-620 HQGS-520 HQGS-220 HQPullulanPEGColumn : Shodex Asahipak GS-HQ seriesEluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CPeptidesGS-HQ columns can be used not only for SEC (GFC) in anaqueous system, but also for multimodal analysis wherehydrophobic interaction and ionic interaction are usedtogether as separation criteria, under certain conditions ofthe eluent. This results in unprecedented separation analysis.GS-320 HQ columns are excellent in the performance forseparating hydrophilic peptides, in particular, acidic or basicpeptides, from each other.MWGlu-Ala-Glu 347 3.12Arg-Asp289 6.75Gly-His-Lys 340 9.95Arg-Pro-Lys-Pro 497 11.44Σf : Hydrophobic parameterpl : Isoelectric pointColumn : Shodex Asahipak GS-320 HQEluent : 30mM Ammonium acetate buffer(pH6.7)Flow rate : 0.5mL/minDetector : UV(220nm)Column temp. : 30˚CplΣf0.390.680.293.240 5 101234Sample : 20μL1. Glu-Ala-Glu 0.025%2. Arg-Asp 0.05%3. Gly-His-Lys 0.025%4. Arg-Pro-Lys-Pro 0.025%15 20 25 30minStandard AnalysisColumnsMultimode ColumnsLow molecular weight water-soluble dietary fiberBy using GS-220 HQ, monosaccharides, disaccharides,and sugar alcohols elute after indigestible componentfraction (indicated by an arrow on the chromatogram).This separation makes the method preferable for thequantification of low molecular weight water-solubledietary fiber.Analysis of purine bases in beerPurine in foods is analyzed as purine base after a step of sample preparation; homogenization,freeze drying, hydolyzation with 70% perchloric acid, and neutralization. Examples below showthe analysis of purin in regular beer and beer treated with guanase (an enzyme that degradesguanine to xanthine). The following data indicates that guanine was decreased and xanthine wasincreased by guanase.Normal beerGuanase treated beerguaninexanthinexanthine1015 20 25 30 35 40 45 50min010 20 30 40 50 min 0 10 20 30 40 50 minColumn : Shodex Asahipak GS-220 HQ x 2Eluent : H 2 OFlow rate : 0.5mL/minDetector : RIColumn temp. : 60˚CColumn : Shodex Asahipak GS-320 HQEluent : 150mM Sodium phosphate buffer(pH2.5)Flow rate : 0.6mL/minDetector : UV(260nm)Column temp. : 35˚CData provided by Kiyoko Kaneko Ph.D.,Faculty of Pharmaceutical Sciences,Teikyo University“Umami”Lignosulfonic acidGelatin analysis with PAGI method1235Sample : 50μg/mL, 20μL1. IMP2. GMP3. AMP4. Inosine5. Hypoxanthine6. Guanosine7. Guanine8. Adenosine9. AdenineSample : 100μLLignosulfonic acid sodium salt 0.1%Sample : 100μLGelatin(Koepff 16922) 0.2%467 890 5 10 15 20 25 30 35 40min0 5 10 15 20 25 30 35 40min5 10 15 20 25 30 3540minColumn : Shodex Asahipak GS-320 HQEluent : 10mM NaH 2 PO 4 aq./10mM Na 2 HPO 4 aq.=1000/31Flow rate : 1.0mL/minDetector : UV(260nm)Column temp. : 40˚CColumn : Shodex Asahipak GS-520 HQ x 2Eluent : 20mM Na 2 HPO 4 aq.Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 40˚CColumn : Shodex Asahipak GS-620 7G-P x 2Eluent : 0.1M KH 2 PO 4 aq./0.1M Na 2 HPO 4 aq.=50/50Flow rate : 1.0mL/minDetector : UV(230nm)Column temp. : 50˚C33

Standard AnalysisColumnsAqueous/Organic SEC ColumnsGF-HQFeaturesPolymer-based SEC columns exhibit high solvent durabilitySupports both aqueous and organic solventsNewsNo.2Semi-microMicro ColumnsPreparativeColumnsp.76p.82Aqueous/Organic SEC ColumnsNEWGF-310GF-210 HQStandard columnsProduct CodeF7600000F7600001F7600002F7600003F7600004F6710018NewsProduct NameNo.2NEW Asahipak GF-210 HQAsahipak GF-310 HQAsahipak GF-510 HQAsahipak GF-710 HQAsahipak GF-7M HQAsahipak GF-1G 7BColumn for low molecular weight substance analysis with exclusion limitof 9,000 (pullulan)Semi-micro columns for Asahipak GF-310 HQEffective for drug analysis in biological samples by using column switchingmethod to remove proteinsUseful for LC/MS analysisSuitable for the analysis of surfactantsPlate Number(TP/column)≥ 19,000≥ 19,000≥ 19,000≥ 11,000≥ 13,000(guard column)Exclusion Limit(Pullulan)9,00040,000300,000(10,000,000)*(10,000,000)*−ParticleSize(µm)555999MaximumPore Size(Å)1804002,00010,00010,000−Column Size(mm)I.D. x Length7.5 × 3007.5 × 3007.5 × 3007.5 × 3007.5 × 3007.5 × 50Shipping SolventH 2 OH 2 O/CH 3 OH=70/30H 2 O/CH 3 OH=70/30H 2 O/CH 3 OH=70/30H 2 O/CH 3 OH=70/30H 2 O/CH 3 OH=70/30GF-7M HQ is a mixed-gel column capable of analyzing samples over a wide range of molecular weight.Usable solventsSolventWater(0-0.5M sodium concentration)MethanolEthanolAcetonitrileTHFGF-210 HQGF-310 HQGF-510 HQGF-710 HQGF-7M HQSolventDMFAcetoneChloroformEthylacetateDMSOGF-210 HQGF-310 HQGF-510 HQGF-710 HQGF-7M HQ0~50%* When replacing acetonitrile, ethyl acetate or chloroform with water, replace with methanol first and then replace with water.* When replacing water with ethyl acetate or chloroform, replace with methanol first and then replace with the required eluent condition.Base Material : Polyvinyl alcoholUsable pH range : pH2-9( )* Estimated value(Note)The usable solvents for preparativecolumns of GF-710 HQ, GS-710 20Fand 20G, are water and methanol.GSM-700 20F or 20G is recommendedwhen other solvents are required.Molecular weight range with pullulan(eluent : ultrapure water)Molecular weight (Pullulan)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8GF-210 HQGF-310 HQGF-510 HQGF-710 HQGF-7M HQMolecular weight range with PEG/PEO(eluent : DMF)Molecular weight (PEG/PEO)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8GF-210 HQGF-310 HQGF-510 HQGF-710 HQGF-7M HQSemi-micro columnsProduct CodeProduct NamePlate Number(TP/column)ParticleSize(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LengthShippingSolventF7600100MSpak GF-310 4B≥ 3,00054004.6 × 50H 2 OF7600110MSpak GF-310 4D≥ 10,00054004.6 × 150H 2 O34F7600024F7600120MSpak GF-310 4EMSpak GF-310 2D≥ 16,000≥ 5,500554004004.6 × 2502.0 × 150H 2 OH 2 OCalibrationStandardsSee page 51 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Calibration curves for GF-HQ series usingpullulanMolecular weight (Pullulan)10 7 Elution volume (mL)10 610 510 410 3GF-210 HQGF-510 HQGF-710 HQGF-310 HQGF-7M HQCalibration curves for GF-HQ series usingPEG/PEOMolecular weight (PEG/PEO)10 7 Elution volume (mL)10 610 510 410 3GF-510 HQGF-210 HQGF-310 HQGF-710 HQGF-7M HQPolyacrylonitrileSample : Polyacrylonitrile 0.1%(w/v), 100μLStandard AnalysisColumns10 2103 4 5 6 7 8 9 10 11Column : Shodex Asahipak GF-HQ seriesEluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CComparison of polystyrenes separation using various solvents3 4Sample : Polystyrene 1mg/mL each, 50μL1. Mw : 1,090,0002. Mw : 190,0003. Mw : 37,9004. Mw : 9,1005. Mw : 2,9803245 6 5 61 6. Mw : 500(A) THF(B) Chloroform (C) DMF1123245610 2103 4 5 6 7 8 9 10 11Column : Shodex Asahipak GF-HQ seriesEluent : DMFFlow rate : 0.6mL/minDetector : RIColumn temp. : 40˚CSodium polystyrene sulfonatesMW:104,0000 10 20 30 minMW:38,0000 10 20 min0 5 10 15 20 25 30 35 40minColumn : Shodex Asahipak GF-710 HQ x 2Eluent : 20mM LiBr in DMFFlow rate : 0.6mL/minDetector : RIColumn temp. : 40˚CPolymers having both hydrophobicand hydrophilic functional groupsmay exhibit hydrophobic interactionswith packing materials.When analyzing such polymers, thehydrophobic interaction can besuppressed by adding organicsolvents to the eluent.Sample : 1mg/mL each, 50μLSodium polystylene sulfonateCH2CHSO3NanAqueous/Organic SEC Columns0 20 min 0 20 min 0 20 minColumn : Shodex Asahipak GF-510 HQ + GF-310 HQEluent : (A); THF, (B); Chloroform, (C); DMFFlow rate : 0.5mL/minDetector : (A),(B) UV(254nm), (C) UV(270nm)Column temp. : 30˚C0MW:8,00010 20 30 minColumn : Shodex AsahipakGF-510 HQEluent : 50mM LiCl aq./CH 3 CN=60/40Flow rate : 0.6mL/minDetector : UV(254nm)Column temp. : 30˚CComparison of two GF columns for the separation performanceof maltooligosaccharidesComparison of two GF columns for the separation performanceof triglyceridesGF-210 HQ demonstrates an improved separation of low molecularsubstances. Below example shows that the peaks obtained by GF-210 HQ areseparated with deeper notches compared to peaks obtained by GF-310 HQ.GF-210 HQ is capable of separating oligosaccharides (trisaccharides tohexasaccharides) while those oligosaccharides were eluted all together whenanalyzed by GF-310 HQ.GF-210 HQ x 2213456Sample : 0.1% each, 20μL1. Maltohexaose2. Maltopentaose3. Maltotetraose4. Maltotriose5. Maltose6. GlucoseSample : 50μL1. Tristearin 0.1%2. Tricaprin 0.2%3. Tricaproin 0.2%4. Tributyrin 1.0%GF-210 HQ212431~344356GF-310 HQ1GF-310 HQ x 21520 25 30 35 min0 5 10 15 minColumn : Shodex Asahipak GF-210 HQ x 2, GF-310 HQ x 2Eluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 50˚CColumn : Shodex Asahipak GF-210 HQ, GF-310 HQEluent : ChloroformFlow rate : 0.6mL/minDetector : RIColumn temp. : 50˚C35

Standard AnalysisColumnsOrganic SEC (GPC) Columns (General Analysis) :THFFeaturesKF-800Standard organic solvent SEC (GPC) columnSupports a wide range of applications from low to high molecular weightcompoundsPreparativeColumnsp.82, 83Standard columnsOrganic SEC (GPC) Columns (General Analysis) : THFKF-800 series : Shipping solvent Tetrahydrofuran (THF)Product CodeF6028010F6028020F6028025F6028030F6027030F6028040F6027040F6028050F6027050F6028060F6028090F6027060F6028070F6027070Product NameGPC KF-801GPC KF-802GPC KF-802.5GPC KF-803GPC KF-803LGPC KF-804GPC KF-804LGPC KF-805GPC KF-805LGPC KF-806GPC KF-806MGPC KF-806LGPC KF-807GPC KF-807LPlate Number(TP/column)≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 11,000≥ 11,000≥ 11,000≥ 13,000≥ 11,000≥ 6,000≥ 6,000Exclusion Limit(Polystyrene)1,5005,00020,00070,00070,000400,000400,0004,000,0004,000,000(20,000,000)*(20,000,000)*(20,000,000)*(200,000,000)*(200,000,000)*Particle Size(µm)666667710101010101818MaximumPore Size(Å)501503005005001,5001,5005,0005,00010,00010,00010,00020,00020,000Column Size(mm)I.D. x Length8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 300F6700300GPC KF-G(guard column)−8−4.6 × 10F6709350GPC KF-800Dsolvent-peakseparation column−10−8.0 × 100* See page 40 for details of the solvent-peak separation columns. The columns with ‘L’ or ‘M’ atthe end of column names are mixed-gel column capable of analyzing samples over a wide rangeof molecular weight distribution.Base Material : Styrene divinylbenzene copolymer( )* Estimated value* See page 50 for applicability of SEC (GPC) columns to solvent replacement.Molecular weight range with polystyrene (eluent : THF)Molecular weight (Polystyrene)10 10 210 3 10 4 10 5 10 6 10 7 10 8 10 9KF-801KF-802KF-802.5KF-803KF-804KF-805KF-806KF-807KF-803LKF-804LKF-805LKF-806MKF-806LKF-807L36CalibrationStandardsSee page 51 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Calibration curves for KF-800 seriesusing polystyreneMolecular weight (Polystyrene)10 810 710 610 510 410 310 2105 6 7 8 9 10 11 12 13Elution volume (mL)KF-802KF-801KF-806KF-804KF-803KF-802.5KF-807KF-8051 2KF-806MColumn : Shodex GPC KF-800 seriesEluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : Room temp.Standard polystyrenes3457Calibration curves for KF-800L (linear type)series using polystyreneMolecular weight (Polystyrene)10 810 710 610 510 410 310 2105 6 7 8 9 10 11 12 13Elution volume (mL)KF-807LKF-806LKF-805LKF-804LKF-803LColumn : Shodex GPC KF-800L seriesEluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : Room temp.Sample : 50μL1. Polystyrene (Mw : 133,000) 0.1%2. Polystyrene (Mw : 55,100) 0.1%3. Polystyrene (Mw : 19,600) 0.1%4. Polystyrene (Mw : 7,210) 0.1%5. Polystyrene (Mw : 3,070) 0.1%6. Polystyrene (Mw : 580) 0.1%7. Toluene (Mw: 92) 0.15%2345Fat-soluble vitamins56123Sample : 100μL1. Vitamin A palmitate500μg/mL2. Vitamin E 50μg/mL3. Vitamin D3 50μg/mL4. Vitamin K3 50μg/mL410 15 20 25minColumn : Shodex GPC KF-801 x 2Eluent : THFFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 40˚CSample : 50μL1. Polystyrene (Mw : 19,825,000) 0.05%2. Polystyrene (Mw : 1,320,000) 0.1%3. Polystyrene (Mw : 133,000) 0.1%4. Polystyrene (Mw : 7,210) 0.1%5. Polystyrene (Mw : 580) 0.1%6. Toluene (Mw : 92) 0.1%Standard AnalysisColumnsOrganic SEC (GPC) Columns (General Analysis) : THF610 5 10 15 20 25 30 min 0 5 10 15 20 25 30 minColumn : Shodex GPC KF-803L x 2Eluent : THFFlow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 40˚CColumn : Shodex GPC KF-807L x 2Eluent : THFFlow rate : 1.0mL/minDetector : UV(254nm)Column temp. : 40˚CStyrene isoprene ABA block copolymerPolycarbonate resinRaw rubberSample : Styrene isoprene ABA block copolymerSample : Polycarbonate resin 0.1%, 100μLSample : Rubber 0.1%, 300μLCH3O C O CCH3 O n15 20 25 min15 20 25min0 10 20 30 minColumn : Shodex GPC KF-806M x 2Eluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : 30˚CColumn : Shodex GPC KF-806L x 2Eluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex GPC KF-806M x 2+ KF-802Eluent : TolueneFlow rate : 1.0mL/minDetector : RIColumn temp. : Room temp.37

Standard AnalysisColumnsOrganic SEC (GPC) Columns (General Analysis) :ChloroformK-800FeaturesStandard organic solvent SEC (GPC) columnSupports a wide range of applications from low to high molecular weight compoundsPreparativeColumnsp.83Standard columnsOrganic SEC (GPC) Columns (General Analysis) : ChloroformK-800 series : Shipping solvent ChloroformProduct CodeF6028110F6028120F6028125F6028130F6028194F6028140F6028195F6028150F6028196F6028160F6028190F6028197F6028170F6028198F6700401F6709450Product NameGPC K-801GPC K-802GPC K-802.5GPC K-803GPC K-803LGPC K-804GPC K-804LGPC K-805GPC K-805LGPC K-806GPC K-806MGPC K-806LGPC K-807GPC K-807LGPC K-GGPC K-800DPlate Number(TP/column)≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 11,000≥ 11,000≥ 11,000≥ 13,000≥ 11,000≥ 6,000≥ 6,000(guard column)solvent-peakseparation columnExclusion Limit(Polystyrene)1,5005,00020,00070,00070,000400,000400,0004,000,0004,000,000(20,000,000)*(20,000,000)*(20,000,000)*(200,000,000)*(200,000,000)*−* See page 40 for details of the solvent-peak separation columns. The columns with ‘L’ or ‘M’ atthe end of column names are mixed-gel column capable of analyzing samples over a wide rangeof molecular weight distribution.−Particle Size(µm)666667710101010101818810MaximumPore Size(Å)501503005005001,5001,5005,0005,00010,00010,00010,00020,00020,000−−Column Size(mm)I.D. x Length8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3004.6 × 108.0 × 100Base Material : Styrene divinylbenzene copolymer( )* Estimated value* See page 50 for applicability of SEC (GPC) columns to solvent replacement.Molecular weight range with polystyrene (eluent : chloroform)Molecular weight (Polystyrene)10 10 210 3 10 4 10 5 10 6 10 7 10 8 10 9K-801K-802K-802.5K-803K-804K-805K-806K-807K-803LK-804LK-805LK-806MK-806LK-807L38CalibrationStandardsSee page 51 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Epoxy resinSample : Epikote ® 1001 0.1%, 100μLLow molecular polydimethylsiloxanesSample : 0.1% each, 100μL1. Polydimethylsiloxane (MW:770)2. Polydimethylsiloxane (MW:237)3. Hexamethyldisiloxane (MW:162)23Standard AnalysisColumns10 15 20 minColumn : Shodex GPC K-803L x 2Eluent : ChloroformFlow rate : 1.0mL/minDetector : UV(254nm)Column temp. : Room temp.Silicon oilSample : Silicone oil 0.1%, 200μLSilicone oil0 510Column : Shodex GPC K-801 x 2Eluent : ChloroformFlow rate : 1.0mL/minDetector : RI(Polarity : negative)Column temp. : 40˚CBionolle TM(Polybutylene succinate/adipate)115 20 25minSample : Polybutylene succinate/adipate 0.2%, 100μLOrganic SEC (GPC) Columns (General Analysis) : Chloroform10 152025 min15 20 25 30 minColumn : Shodex GPC K-806M x 2Eluent : TolueneFlow rate : 1.0mL/minDetector : RIColumn temp. : 45˚CColumn : Shodex GPC K-806M x 2 + K-801Eluent : ChloroformFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CRecycling fractionation of lauryl stearyl thiodipropionateSample : 5%, 500μL1. Distearyl thiodipropionate2. Lauryl stearyl thiodipropionate3. Dilauryl thiodipropionateCYCLE1CYCLE2 CYCLE6 CYCLE10123Column : Shodex GPC K-LG + K-2001Eluent : ChloroformFlow rate : 3.0mL/minDetector : RI(preparative type)Column temp. : 50˚C0 10 20 min* See page 83 for K-200139

Standard AnalysisColumnsOrganic SEC (GPC) Columns (General Analysis) :DMFFeaturesKD-800Standard organic solvent SEC (GPC) columnSupports a wide range of applications from low to high molecular weight compoundsOrganic SEC (GPC) Columns (General Analysis) : DMF Solvent-peak Separation Columns for Organic SEC (GPC)Standard columnsKD-800 series : Shipping solvent Dimethylformamide (DMF)Product CodeF6028210F6028220F6028225F6028230F6028240F6028250F6028260F6028290F6028270F6700411Molecular weight range with PEG/PEO (eluent : DMF)Molecular weight (PEG/PEO)10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9KD-801KD-802KD-802.5KD-803KD-804KD-805KD-806MKD-806KD-807Solvent-peak Separation Columns forOrganic SEC (GPC)FeaturesKF-800D, K-800DProduct NameGPC KD-801GPC KD-802GPC KD-802.5GPC KD-803GPC KD-804GPC KD-805GPC KD-806GPC KD-806MGPC KD-807GPC KD-GPlate Number(TP/column)≥ 17,000≥ 17,000≥ 17,000≥ 17,000≥ 17,000≥ 11,000≥ 11,000≥ 13,000≥ 6,000(guard column)* The columns with ‘M’ at the end of column names are mixed-gel column capable ofanalyzing samples over a wide range of molecular weight distribution.* See page 50 for applicability of SEC (GPC) columns to solvent replacement.Exclusion Limit(PEG/PEO)*2,5005,00020,00070,000400,0004,000,000(20,000,000)**(20,000,000)**(200,000,000)**−Particle Size(µm)66667101010188MaximumPore Size(Å)501503005001,5005,00010,00010,00020,000−Column Size (mm)I.D. x Length8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3004.6 × 10Base Material : Styrene divinylbenzene copolymer*PEG : polyethylene glycol*PEO : polyethylene oxide( )** Estimated valueUse this column in combination with a linear columnBy shifting the elution of monomers, polymer additives, and the solvent-peak in low molecularregion, it reduces interferences in the calculation of the molecular weight distribution ofpolymers and oligomersSolvent-peak separation columnsProduct CodeProduct NameColumn CombinationParticle Size(µm)Column Size (mm)I.D. x LengthShipping SolventF6709350GPC KF-800DKF-805L, 806L, 806M, 807L108.0 × 100THFF6709450GPC K-800DK-805L, 806L, 806M, 807L108.0 × 100Chloroform40Base Material : Styrene divinylbenzene copolymer*Contact Shodex or our distributors near you for customized columns.

Calibration curves for KD-800 seriesusing PEG/PEOMolecular weight (PEG/PEO)10 8 Elution volume (mL)10 710 610 510 410 3KD-805KD-804KD-803KD-802.5KD-807KD-80610 610 510 410 310 210 7 106KD-802KD-806MPolyvinylpyrrolidonesSample : Polyvinylpyrrolidone(PVP) 0.2% each(A) PVP(K-30) 400μL(B) PVP(K-60) 500μL(C) PVP(K-90) 500μL(A)(B)Melamine formaldehyde resinSample : 30μLMelamine-formaldehyde resin 1%Standard AnalysisColumnsMolecular massKD-8017 8 9 10 11 12Column : Shodex GPC KD-800 seriesEluent : DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚CCelluloses6 78 9 10 11Elution volume (mL)Column : Shodex GPC KD-806MEluent : 1% LiCl in DMIFlow rate : 0.5mL/minDetector : RI, MALS(Multi angle laser light scattering)Column temp. : 60˚C* DMI 1,3-dimethyl-2-imidazolidinonePotato starchSample : 100μLPotato starch in DMSO 0.1%* solved at 80˚C0(C)0 10 20 30 minColumn : Shodex GPC KD-806M x 2Eluent : 10mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚CSample : Cellulose ca. 0.05% each100μLEffects of solvent-peak separation columnSample : Polyvinyl chlorideKF-806LCellulose is known to be difficult todissolve. A cellulose solution isprepared by repeating solventreplacement. It is reported that thelong time required for dissolution(1 to 60 days), depends on solventtype, the crystallinity andmolecular weight of the sample.Data provided by Dr. Masahiko Yanagisawa,Isogai group,Graduate School of Agricultural and Life Sciences,The University of TokyoDOP(Dioctyl phthalate)5 10 15 minMolecular weight (Polystyrene)10 6 Retention volume (mL)10 510 410 3101515Phenoxy resinKF-806L2025 minColumn : Shodex GPC KD-802 x 2Eluent : 10mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚C20 25 minColumn : Shodex GPC KD-806M x 2Eluent : 10mM LiBr in DMFFlow rate : 1.0mL/minDetector : RIColumn temp. : 50˚CKF-806L+KF-800DSample : 250μLPhenoxy resin 0.2%10 2 5 6 7 8 9 10 11 12 13 14 15Organic SEC (GPC) Columns (General Analysis) : DMF Solvent-peak Separation Columns for Organic SEC (GPC)0 5 10 15minKF-806L + KF-800DColumn : Shodex GPC KD-806MEluent : 10mM LiBr in (DMSO/DMF=75/25)Flow rate : 1.0mL/minDetector : RIColumn temp. : 50˚C0 5 10 minColumn : Shodex GPC KF-806LGPC KF-806L + KF-800DEluent : THFFlow rate : 1.0mL/minDetector : RI41

Standard AnalysisColumnsOrganic SEC (GPC) Columns :Rapid Analysis, High Performance AnalysisFeaturesKF-600Approximately half of the analysis time compared with standard columnsThe amount of solvent used is reduced to about a thirdImproved applicability of solvent replacementOrganic SEC (GPC) Columns : Rapid Analysis, High Performance AnalysisKF-400HQF6028091F6028092F6028093F6028094F6028095F6028096F6028097F6028098F6028099F6700300About 1.5 times better separation performance than standard columns, obtains higher resolutionAbout 4 times better sensitivity than that of standard columns, supports high sensitivity analysisThe amount of solvent used is reduced to about a thirdNewsImproved applicability of solvent replacementNo.3, 10Rapid analysis downsized columnsKF-600 seriesProduct CodeProduct NameGPC KF-601GPC KF-602GPC KF-602.5GPC KF-603GPC KF-604GPC KF-605GPC KF-606GPC KF-606MGPC KF-607GPC KF-GPlate Number(TP/column)≥ 17,000≥ 17,000≥ 17,000≥ 17,000≥ 16,000≥ 7,000≥ 7,000≥ 8,000≥ 5,000(guard column)Use of the KF-600 series with semi-micro type devices is recommended.Exclusion Limit(Polystyrene)1,5005,00020,00070,000400,0004,000,000(20,000,000)*(20,000,000)*(200,000,000)*−* The columns with ‘M’ at the end of column names are mixed-gel column capable ofanalyzing samples over a wide range of molecular weight distribution.* See page 50 for applicability of SEC (GPC) columns to solvent replacement.High performance semi-micro columnsKF-400HQ seriesProduct CodeF6028111F6028112F6028114Product NameGPC KF-401HQGPC KF-402HQGPC KF-402.5HQPlate Number(TP/column)≥ 25,000≥ 25,000≥ 25,0001,5005,00020,000Particle Size(µm)33333101010188Maximum Pore Size(Å)501503005001,5005,00010,00010,00020,000−Column Size (mm)I.D. x Length6.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1506.0 × 1504.6 × 10Base Material : Styrene divinylbenzene copolymerShipping Solvent : Tetrahydrofuran (THF)( )* Estimated valueUse of the KF-400HQ series with semi-micro type devices is recommended.Exclusion Limit(Polystyrene)Particle Size(µm)333Maximum Pore Size(Å)50150300Column Size (mm)I.D. x Length4.6 × 2504.6 × 2504.6 × 250F6028116GPC KF-403HQ≥ 25,00070,00035004.6 × 250F6028118GPC KF-404HQ≥ 25,000400,00031,5004.6 × 250F6028119GPC KF-405LHQ≥ 10,0004,000,000105,0004.6 × 250F6028122GPC KF-406LHQ≥ 10,000(20,000,000)*1010,0004.6 × 250F6700300GPC KF-G(guard column)−8−4.6 × 10* The columns with ‘L’ at the end of column names are mixed-gel column capable ofanalyzing samples over a wide range of molecular weight distribution.* See page 50 for applicability of SEC (GPC) columns to solvent replacement.Base Material : Styrene divinylbenzene copolymerShipping Solvent : Tetrahydrofuran (THF)( )* Estimated valueMolecular weight range with polystyrene (eluent : THF)Molecular weight (Polystyrene)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9KF-601KF-602KF-602.5KF-603KF-604KF-605KF-606Molecular weight (Polystyrene)10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9KF-401HQKF-402HQKF-402.5HQKF-403HQKF-404HQKF-405LHQKF-406LHQKF-607KF-606M42CalibrationStandardsSee page 51 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Calibration curves for KF-600 series using polystyreneMolecular weight (Polystyrene)10 710 610 510 410 310 2KF-604KF-603KF-602.5KF-602KF-601KF-606KF-605KF-606M1.0 1.5 2.0 2.5 3.0 3.5 4.0Elution volume (mL)Column : Shodex GPC KF-600 seriesEluent : THFFlow rate : 0.5mL/minDetector : UV(254nm)Column temp. : Room temp.Comparison of standard, rapid analysis, and high performance type columnsStandard typeKF-802.5 x 250µL injectionHigh performance typeKF-402.5HQ x 210µL injectionRapid analysis typeKF-602.5 x 210µL injection6.0mL5.7mL21mLCalibration curves for KF-400HQ series using polystyreneMolecular weight (Polystyrene)10 610 510 410 310 2101.0 1.5 2.0 2.5 3.0 3.5 4.010 7 Elution volume (mL)KF-404HQKF-403HQKF-402.5HQKF-401HQKF-402HQColumn : Shodex GPC KF-400HQ seriesEluent : THFFlow rate : 0.3mL/minDetector : UV(254nm)Column temp. : Room temp.Sample : EPON1001 0.2%By using KF-602.5, the analysis time is reduced to less than halfof that of KF-802.5. Thus KF-600 series enables rapid analysis.On the other hand, KF-402.5HQ has a theoretical plate number1.5 times larger than that of the standard column, therebyimproving resolution especially in the analysis of molecules thathave a small to medium molecular weight. Rapid analysis andhigh performance type columns use less than one third ofsolvent per analysis compared to standard type columns.Column : Shodex GPC KF-802.5 x 2GPC KF-402.5HQ x 2GPC KF-602.5 x 2Eluent : THFFlow rate : (KF-802.5) 1.0mL/min(KF-402.5HQ) 0.3mL/min(KF-602.5) 0.6mL/minDetector : (KF-802.5) RI(conventional type)(KF-402.5HQ, KF-602.5) RI(small cell volume)Column temp. : 40˚CStandard AnalysisColumnsOrganic SEC (GPC) Columns : Rapid Analysis, High Performance Analysis0510 15 20 25 minStyrene acrylonitrile copolymerLiquid paraffinPhthalatesSample : Styrene-acrylonitrile (30:70) copolymerSample : Liquid paraffin 1%, 5μLSample : 10μL1. Dioctyl phthalate 0.1%2. Dibutyl phthalate 0.1%3. Dipropyl phthalate 0.1%4. Diethyl phthalate 0.1%5. Dimethyl phthalate 0.1%6. Propylbenzene 0.2%7. Ethylbenzene 0.2%8. Toluene 0.2%9. Benzene 0.2%456 8 9 73216 8 10 12 14min2 4 6 8 10 min812 16 20 minColumn : Shodex GPC KF-606M x 2Eluent : 10mM LiBr in DMFFlow rate : 0.5mL/minDetector : RI(small cell volume)Column temp. : 40˚CColumn : Shodex GPC KF-401HQEluent : ChloroformFlow rate : 0.3mL/minDetector : RI(small cell volume)Column temp. : 40˚CColumn : Shodex GPC KF-401HQ x 2Eluent : THFFlow rate : 0.3mL/minDetector : UV(254nm) (small cell volume)Column temp. : 40˚C43

Standard AnalysisColumnsOrganic SEC (GPC) Columns : Linear Calibration TypeOrganic SEC (GPC) Columns :Linear Calibration TypeLFFeaturesEmploys a special packing material with a wide pore size distribution (multi pore type)Highly linear calibration curve without inflection pointsMolecular weight distribution can be determined with high precisionEnables analysis over a broad range of molecular weights (100 to 2,000,000)Column for rapid analysis (LF-604) and column for high performance analysis (LF-404) enabling reduction insolvent use are also availableNotebook No.1 News No.4, 40Standard columnProduct CodeF6021041F6709621Product NameGPC LF-804GPC LF-G* See page 50 for applicability of SEC (GPC) columns to solvent replacement.Rapid analysis downsized columnProduct CodeF6021042F6709621Product NameGPC LF-604GPC LF-GPlate Number(TP/column)≥ 17,000(guard column)Plate Number(TP/column)≥ 9,000(guard column)Exclusion Limit(Polystyrene)2,000,000−Particle Size(µm)66MaximumPore Size(Å)3,000−Column Size (mm)I.D. x Length8.0 × 3004.6 × 10Base Material : Styrene divinylbenzene copolymerShipping Solvent : Tetrahydrofuran (THF)Use of the LF-604 with semi-micro type devices is recommended.2,000,000−* See page 50 for applicability of SEC (GPC) columns to solvent replacement. Base Material : Styrene divinylbenzene copolymerShipping Solvent : Tetrahydrofuran (THF)High performance semi-micro columnProduct CodeF6021043Product NameGPC LF-404Plate Number(TP/column)≥ 14,000Exclusion Limit(Polystyrene)Particle Size(µm)66MaximumPore Size(Å)3,000−Column Size (mm)I.D. x Length6.0 × 1504.6 × 10Use of the LF-404 with semi-micro type devices is recommended.Exclusion Limit(Polystyrene)2,000,000Particle Size(µm)6MaximumPore Size(Å)3,000Column Size (mm)I.D. x Length4.6 × 250F6709621GPC LF-G(guard column)−6−4.6 × 10* See page 50 for applicability of SEC (GPC) columns to solvent replacement. Base Material : Styrene divinylbenzene copolymerShipping Solvent : Tetrahydrofuran (THF)Molecular weight range with polystyrene (eluent : THF)Molecular weight (Polystyrene)10 10 2 10 3 10 4 10 5 10 6 10 7LF-804, 604, 404CalibrationStandardsSee page 51 forCalibration StandardsScheme of linear calibration type packingConnecting linear calibration type columns (LF series)The linear calibration type columns cover a broad rangeof molecular weights with only one kind of packing (column).Connecting mixed-gel columns (KF-804L, etc.)Connecting different grades columns(KF-804 + KF-803 + KF-802, etc.)44*Contact Shodex or our distributors near you for customized columns.

Calibration curve for LF-804 usingpolystyreneMolecular weight (Polystyrene)10 710 610 510 410 310 2 5 6 7 8 9 10 11 1210 8 Elution volume (mL)Column : Shodex GPC LF-804Eluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CXylanSample : Xylan 0.1%, 100μLCalibration curves for LF-604 and LF-404using polystyreneMolecular weight (Polystyrene)10 710 610 510 4LF-40410 3LF-60410 21.0 1.5 2.0 2.5 3.0 3.5 4.0Elution volume (mL)Column : Shodex GPC LF-604, LF-404Eluent : THFFlow rate : (LF-604) 0.5mL/min(LF-404) 0.3mL/minDetector : RI(small cell volume)Column temp. : 40˚CPolyamide (Nylon6/6)Sample : Nylon ® 6/6 0.1%, 20μLPolyurethaneSample : Polyurethane 0.1%, 20μL0 5 10 15 20 minColumn : Shodex GPC LF-404 x 2Eluent : THFFlow rate : 0.3mL/minDetector : RI(small cell volume)Column temp. : 40˚CPolymethyl methacrylateSample : 100μLPolymethyl methacrylateStandard AnalysisColumnsOrganic SEC (GPC) Columns : Linear Calibration Type0 5 10 15 20 25 min 0 5 10 15 20 25min0 5 10 15 20 25 30minColumn : Shodex GPC LF-804Eluent : 20mM H 3 PO 4 + 20mM LiBr in(DMSO/DMF=80/20)Flow rate : 0.6mL/minDetector : RIColumn temp. : 50˚CColumn : Shodex GPC LF-404Eluent : 5mM CF 3 COONa in HFIPFlow rate : 0.15mL/minDetector : RI(small cell volume)Column temp. : 40˚CColumn : Shodex GPC LF-804 x 2Eluent : Methyl ethyl ketoneFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CPolyamic acidComparison of polystyrenes separation with different numbers of LF-404Sample : Poly(pyromellitic dianhydride-co-4,4'-oxydianiline), 100μL45LF-404 x 136721Sample : 10μL1. PS Mw : 1,030,0002. PS Mw : 152,0003. PS Mw : 66,0004. PS Mw : 22,0005. PS Mw : 5,0506. PS Mw : 5807. Ethylbenzene4LF-404 x 2132567Column : Shodex GPC LF-404 x nEluent : THFFlow rate : 0.3mL/minDetector : RI(small cell volume)Column temp. : 40˚C0 5 10 15 20 25 30 35 40 45minColumn : Shodex GPC LF-804 x 2Eluent : 30mM LiBr + 30mM H 3 PO 4 in NMPFlow rate : 0.7mL/minDetector : RIColumn temp. : 50˚CLF-404 x 30 5435726110 15 20 25 30 min45

Standard AnalysisColumnsOrganic SEC (GPC) Columns :High Temperature/Ultra High Temperature AnalysisHT-800UT-800FeaturesVaried product lineup to support a wide range of molecular weightsDedicated to SEC analysis at high/ultra high temperatures with a maximum usable temperature of 210˚CSuitable for the analysis of ultra high molecular weight polymer containing samplesOrganic SEC (GPC) Columns : High Temperature/Ultra High Temperature AnalysisStandard columnsProduct CodeF6208700F6208710F6208720F6208730F6208740F6208770F6709410F6208600F6208610F6208620F6709400F6208390F6700280Product NameGPC HT-803GPC HT-804GPC HT-805GPC HT-806GPC HT-806MGPC HT-807GPC HT-GGPC UT-802.5GPC UT-806MGPC UT-807GPC UT-GGPC AT-806MSGPC AT-GPlate Number(TP/column)≥ 7,000≥ 7,000≥ 7,000≥ 7,000≥ 7,000≥ 4,000(guard column)≥ 4,400≥ 4,400≥ 3,300(guard column)≥ 6,000(guard column)Exclusion Limit *(Polystyrene)70,000400,0004,000,000(20,000,000)**(20,000,000)**(200,000,000)**Molecular weight range with polystyrene (eluent : THF)−20,000(20,000,000)**(200,000,000)**−(20,000,000)*** The columns with ‘M’ at the end of column names are mixed-gel column capable ofanalyzing samples over a wide range of molecular weight distribution.−UsableTemperature(˚C)100−150100−150100−150100−150100−150100−150100−150100−210100−210100−210100−210***Ta −150***Ta −150Particle Size(µm)13131313131813303030301215MaximumPore Size(Å)5001,5005,00010,00010,00020,000−30010,00020,000−10,000−Column Size(mm)I.D. x Length8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 508.0 × 3008.0 × 3008.0 × 3008.0 × 508.0 × 2508.0 × 50Base Material : Styrene divinylbenzene copolymerShipping Solvent : Toluene*Exclusion limit was measured with THF( )** Estimated valueTa*** : Ambient temperatureMolecular weight (Polystyrene)10 10 210 3 10 4 10 5 10 6 10 7 10 8 10 9HT-803HT-804HT-805HT-806MHT-806HT-807UT-802.5UT-806MUT-807AT-806MS46CalibrationStandardsSee page 51 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.

Calibration curves for HT-800 series usingpolystyreneMolecular weight (Polystyrene)10 710 610 510 410 310 2105 6 7 8 9 10 11 12 13Elution volume (mL)Molecular weightHT-804HT-806HT-803HT-805HT-806MColumn : Shodex GPC HT-800 seriesEluent : THFFlow rate : 1.0mL/minDetector : RIColumn temp. : Room temp.High density polyethyleneSample : High density polyethylene (HDPE-B)10 810 7RI10 610 510 41216 20 minEffects of gel particle size in high temperature GPC columnsHigh temperature SEC columns are suitable for the analysis of high molecular weight polymers that aredifficult to be dissolved in ambient temperature solvents; examples of such polymers are polyethyleneand polypropylene. GPC UT-800 series, which are packed with large particle size (30 μm) gel, arerecommended for the analysis of macromolecules. The large particle size prevents potential molecularshear degradation of the sample.15Column : UT-806M x 3(Particle size : 30µm)Vo110120 25Column : AT-806MS x 2(Particle size : 12µm)Vo152230Shear degradation20min3540min10Low density polyethyleneSample : Low density polyethylene (LDPE-C)Column : HT-806M x 2(Particle size : 13µm)1Vo10 15 20 25 min152Shear degradation20Sample :1. Polystyrene(MW : 20,000,000)2. High density polyethylene (HDPE-A)25minColumn : Shodex GPC UT-806MHT-806M, AT-806MSEluent : 0.1% BHT in o-DichlorobenzeneFlow rate : 1.0mL/minDetector : RIColumn temp. : 140˚CStandard AnalysisColumnsOrganic SEC (GPC) Columns : High Temperature/Ultra High Temperature AnalysisColumn : Shodex GPC UT-806M x 2Eluent : 0.1% BHT in o-DichlorobenzeneFlow rate : 1.0mL/minDetector : RI, MALS(Multi angle laser light scattering)Column temp. : 145˚CColumn : Shodex GPC HT-806M x 2Eluent : 0.1% BHT in o-DichlorobenzeneFlow rate : 1.0mL/minDetector : RIColumn temp. : 140˚C47

Standard AnalysisColumnsOrganic SEC (GPC) Columns : HFIPFeaturesHFIP-800 Columns exclusively for use with hexafluoroisopropanol (HFIP)HFIP-600 Rapid analysis, solvent saving typeOrganic SEC (GPC) Columns : HFIPStandard columnsHFIP-800 seriesProduct Code Product NameF6028530 GPC HFIP-803F6028540 GPC HFIP-804F6028550 GPC HFIP-805F6028560 GPC HFIP-806F6028590 GPC HFIP-806MPlate Number(TP/column)≥ 12,000≥ 12,000≥ 10,000≥ 10,000≥ 10,000Exclusion Limit(PMMA)*30,000100,0001,000,000(10,000,000)**(10,000,000)**Particle Size(µm)107101010MaximumPore Size(Å)5001,5005,00010,00010,000Column Size(mm)I.D. x Length8.0 × 3008.0 × 3008.0 × 3008.0 × 3008.0 × 300F6028570GPC HFIP-807≥ 4,000(100,000,000)**1820,0008.0 × 300F6700500GPC HFIP-LG(guard column)−15−8.0 × 50* The columns with ‘M’ at the end of column names are mixed-gel column capable ofanalyzing samples over a wide range of molecular weight distribution.Rapid analysis downsized columnsHFIP-600 seriesBase Material : Styrene divinylbenzene copolymerShipping Solvent : Hexafluoroisopropanol (HFIP)*PMMA : Polymethylmethacrylate( )** Estimated valueUse of the HFIP-600 series with semi-micro type devices is recommended.Product CodeProduct NamePlate Number(TP/column)Exclusion Limit(PMMA)*Particle Size(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LengthF6021030GPC HFIP-603≥ 12,00030,00035006.0 × 150F6021040GPC HFIP-604≥ 12,000100,00031,5006.0 × 150F6021050GPC HFIP-605≥ 5,0001,000,000105,0006.0 × 150F6021060GPC HFIP-606≥ 5,000(10,000,000)**1010,0006.0 × 150F6021080GPC HFIP-606M≥ 6,000(10,000,000)**1010,0006.0 × 150F6021070GPC HFIP-607≥ 3,000(100,000,000)**1820,0006.0 × 150F6700511GPC HFIP-G(guard column)−8−4.6 × 10* The columns with ‘M’ at the end of column names are mixed-gel column capable ofanalyzing samples over a wide range of molecular weight distribution.Base Material : Styrene divinylbenzene copolymerShipping Solvent : Hexafluoroisopropanol (HFIP)*PMMA : Polymethylmethacrylate( )** Estimated valueMolecular weight range with PMMA (eluent : HFIP)48Molecular weight (PMMA)10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9HFIP-803, 603HFIP-804, 604HFIP-805, 605HFIP-806, 606HFIP-807, 607HFIP-806M, 606M*Contact Shodex or our distributors near you for customized columns.CalibrationStandardsSee page 51 forCalibration Standards

Calibration curves for HFIP-800 seriesusing PMMAMolecular weight (PMMA)10 710 610 510 410 310 2105 6 7 8 9 10 11 12Elution volume (mL)HFIP-805HFIP-806MHFIP-804HFIP-803HFIP-806Column : Shodex GPC HFIP-800 seriesEluent : HFIPFlow rate : 1.0mL/minDetector : RIColumn temp. : Room temp.Polyamide (effects of salt addition)Calibration curves for HFIP-600 seriesusing PMMAMolecular weight (PMMA)10 610 510 410 310 2HFIP-605HFIP-606MHFIP-604HFIP-603HFIP-6061.0 1.5 2.0 2.5 3.0 3.5 4.0Elution volume (mL)Column : Shodex GPC HFIP-600 seriesEluent : 5mM CF 3 COONa in HFIPDetector : RI(small cell volume)Column temp. : 40˚CSample : Polycaprolactum (Nylon ® 6)Polyethylene terephthalate (PET)Sample : PET 0.2%, 20μLMw=19,3000 5 1015minColumn : Shodex GPC HFIP-606M x 2Eluent : 5mM CF 3 COONa in HFIPFlow rate : 0.6mL/minDetector : RI(small cell volume)Column temp. : 40˚CPolybutylene terephthalate (PBT)Sample : PBT 0.05%, 500μLStandard AnalysisColumnsOrganic SEC (GPC) Columns : HFIPHFIP5mM CF 3 COONa in HFIPIn SEC analysis using HFIP, some samplesmay yield abnormal peaks as a result of ionicinteraction. In this case, ionic interaction canbe suppressed by adding sodiumtrifluoroacetate to HFIP.10 15 20 2530 min1520 30 minColumn : Shodex GPC HFIP-806M x 2Eluent : HFIP(solid line), 5mM CF 3 COONa in HFIP(broken line)Flow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CColumn : Shodex GPC HFIP-805 + HFIP-803Eluent : 5mM CF 3 COONa in HFIPFlow rate : 1.0mL/minDetector : RIColumn temp. : 40˚CPolyamides (Nylon 6/10 and Nylon 6)PolyacetalSample : Nylon ® 6/10 0.2%, 20μL Sample : Nylon ® 6 0.2%, 20μL Sample : Polyacetal 0.2%, 20μLMw=29,300 Mw=38,100 Mw=176,0000 5 10 15 0 510150 5 10minmin15minColumn : Shodex GPC HFIP-606M x 2Eluent : 5mM CF 3 COONa in HFIPFlow rate : 0.6mL/minDetector : RI(small cell volume)Column temp. : 40˚CColumn : Shodex GPC HFIP-606M x 2Eluent : 5mM CF 3 COONa in HFIPFlow rate : 0.6mL/minDetector : RI(small cell volume)Column temp. : 40˚C49

Standard AnalysisColumnsApplicability of SEC (GPC) Columns to SolventReplacementShipping Solvent : THFProduct NameShipping Solvent : DMFApplicability of SEC (GPC) Columns to Solvent ReplacementSolventTetrahydrofuran(THF)ChloroformCarbon tetrachlorideBenzeneToluenep-Xyleneo-Dichlorobenzene(ODCB)KF-801 KF-802KF-802.5KF-803LKF-804LKF-803 KF-804KF-805KF-806KF-807KF-806MKF-805LKF-806LKF-807LShipping Solvent : ChloroformK-801 K-802 K-803 K-804K-802.5K-805K-803LK-806K-804LK-807K-806MK-805LK-806LK-807LKF-601 KF-603KF-602 KF-604KF-602.5 KF-605KF-606KF-607KF-606MShipping Solvent : THFKF-401HQ KF-403HQKF-402HQ KF-404HQKF-402.5HQ KF-405LHQKF-406LHQLF-804LF-604LF-404KD-801KD-802KD-802.5KD-803KD-804KD-805KD-806KD-807KD-806MTrichlorobenzene(TCB)DioxaneDiethyl etherEthyl acetateAcetoneMethyl ethyl ketoneDimethylformamide(DMF)*1*1*1Dimethylacetamide(DMAc)*1*1*1Hexafluoroisopropanol(HFIP)*1*1m-Cresolo-ChlorophenolQuinolinN-Methylpyrrolidone(NMP)*1*1*1Dimethylsulfoxide(DMSO)*1*1*130% m-Cresol/Chloroform30% o-Chlorophenol/Chloroform30% HFIP/ChloroformHexaneAcetonitrileMethanolWater50: Solvent replacement possible: Solvent replacement possible, but this may cause column performance to deteriorate slightly*1 : Usable at 40˚C or higher: Solvent replacement not possible

Calibration Standards for SEC[Polystyrene (PS)]Kit typeSL-105Std. No. Mp Mw/MnS-22S-13S-10S-6.9S-4.9S-2.9S-2.2S-1.3S-0.7S-0.5FeaturesFor organic solvent SEC (GPC)Less branched polystyrene with anionic polymerizationEasily soluble in tetrahydrofuran (THF), chloroform, toluene, and o-dichlorobenzene (ODCB)Product CodeF8601105F8602105F860307521,80013,00010,4006,9404,9102,9402,1701,280770580Product Name Contents MW RangeSTANDARD SL-105STANDARD SM-105STANDARD SH-751.021.021.031.031.031.041.041.071.081.12SM-105Std. No. Mp Mw/MnS-2704S-1345S-609S-333S-139S-53S-22S-6.9S-3.2S-1.30.5g x 10 kinds0.5g x 10 kinds0.5g x 7 kinds2,700,0001,350,000609,000333,000139,00052,50021,8006,9403,1801,2701.041.031.021.031.031.031.021.031.041.06580-21,8001,270-2,700,000591,000-6,870,000SH-75Std. No. Mp Mw/MnS-6870S-5190S-3990S-2350S-1820S-991S-5916,870,0005,190,0003,990,0002,350,0001,820,000991,000591,0001.091.031.051.041.041.051.02Structural formula of S seriesC 4H 9 CH 2 CH(Note)Molecular weights (Mp, Mw/Mn)of each kit may vary dependingon production lots.nHCalibration Standardsfor SECCalibration Standards for SEC[Polymethylmethacrylate (PMMA)]FeaturesFor organic solvent SEC (GPC)Narrow molecular weight distribution rangeEasily soluble in hexafluoroisopropanol (HFIP) and dimethylformamide (DMF)Kit typeProduct CodeF8604075 STANDARD M-75 0.5g x 7 kinds[Pullulan]Product Name Contents MW Range2,890-955,000(Note)Molecular weights (Mp, Mw/Mn) of eachkit may vary depending on production lots.Std. No. Mp Mw/MnM-955M-509M-202M-63M-20M-6.8M-2.9955,000509,000202,00063,00020,3006,8302,8901.051.031.021.021.031.101.10FeaturesFor aqueous SEC (GFC)Unbranched pullulan standardHigh solubility in water eliminates the possibility of recrystallizationKit typeProduct CodeProduct NameContentsMW RangeStructural formula of P seriesF8400000 STANDARD P-82 0.2g x 8 kindsSingle type5,900-708,000O CH 2 HHHOOHOHHH OHOHCH 2OHH HCH 2OHHHOHOOH H O OH HH OHH OHnProduct CodeProduct NameContentsMpMw/MnF8402500F8401300F8400800F8400400F8400200F8400100F8400050F8400020F8400010F8400005F8400003F8400002F8400001STD P-2500STD P-1300STD P-800STD P-400STD P-200STD P-100STD P-50STD P-20STD P-10STD P-5STD P-3STD P-2STD P-10.2g0.2g0.5g0.5g0.5g0.5g0.5g0.5g0.5g0.5g0.2g0.2g0.2g2,350,0001,220,000708,000344,000200,000107,00047,10021,1009,6005,9002,8902,1501,4201.491.371.271.151.111.131.071.091.091.091.121.111.18Std. No. Mp Mw/MnP-800P-400P-200P-100P-50P-20P-10P-5708,000344,000200,000107,00047,10021,1009,6005,9001.271.151.111.131.071.091.091.09(Note)Molecular weights (Mp, Mw/Mn) of each kitmay vary depending on production lots.51

Standard AnalysisColumnsColumns for Anion Exchange ChromatographyFeaturesQA-825,DEAE-825Suitable for the analysis of relatively high molecular weight compounds,such as proteins, peptides, DNA, and RNAUsable under wide pH range between 2 and 12NotebookNo.7Semi-microMicro ColumnsPreparativeColumnsp.77p.80Columns for Anion Exchange ChromatographyDEAE3N-4TDEAE-2BES-502N 7CWA-624Non-porous base materialFor rapid analysisNotebookNo.6NewsNo.33Non-porous base materialSupports UHPLC (available under hyperbaric conditions up to 30 MPa)NewsNo.42Compared to IEC series columns, polyvinyl alcohol is used as base materialand this offers different separation patternLow hydrophobic interaction of proteins allows analysis under mild conditionsSuitable for anion exchange analysis of low molecular weight compoundssuch as nucleotidesSemi-microMicro ColumnsPreparativeColumnsp.77p.80Standard columnsStrong anion exchange resin (Functional Group : Quaternary ammonium)ProductCodeProduct NameIon ExchangeCapacity(meq/g)Base MaterialParticleSize(µm)PoreSize(Å)Column Size(mm)I.D. x LengthShipping SolventF6110011 IEC QA-825 0.45 Polyhydroxymethacrylate 12 5,000 8.0 × 75 50mM Na 2 SO 4 aq.Weak anion exchange resin (Functional Group : Diethylaminoethyl)ProductCodeProduct NameIon ExchangeCapacity(meq/g)Base MaterialParticleSize(µm)PoreSize(Å)Column Size(mm)I.D. x LengthShipping SolventF6118255IEC DEAE-8250.6Polyhydroxymethacrylate85,0008.0 × 7550mM Na 2 SO 4 aq.F6112100IEC DEAE3N-4T0.4Polyhydroxymethacrylate2.5−4.6 × 35H 2 OF6112110PIKESS DEAE-2B0.4Polyhydroxymethacrylate2.5−2.0 × 50H 2 OF7640002Asahipak ES-502N 7C0.55Polyvinyl alcohol92,0007.5 × 10050mM 1,3-Diaminopropane+ 50mM NaCl(pH10.0)F6356240AXpak WA-6241.2Polyhydroxymethacrylate102,0006.0 × 1500.1M Sodium phosphate buffer(pH3.0)/CH 3 CN=80/20F6700245AXpak WA-G(guard column)Polyhydroxymethacrylate10−4.6 × 100.1M Sodium phosphate buffer(pH3.0)/CH 3 CN=80/2052*Contact Shodex or our distributors near you for customized columns.

Proteins in human serumSample : Human serum 0.5%, 200μL1. IgG42. Transferrin3. (Unknown)4. Serum albumin01122315 30 45 minColumn : Shodex IEC QA-825Eluent : (A); 20mM Tris-HCl buffer(pH8.6)(B); (A) + 0.5M NaClLinear gradient; 100% (A) to 50% (B), 60minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.3 3120 5 10 15 20min1343 420 5 10 15 20minComparison of porous DEAE-825 and non-porous DEAE3N-4T for protein separationporous gel DEAE-825Effects of eluent pH for DEAE-825pH6.0pH8.0IEC DEAE3N-4T is a weak anion exchange column, having diethylaminoethyl functional group modified onnon-porous gel. The non-porous gel enables rapid analysis of proteins and peptides. DEAE3N-4T is alsosuitable for the analysis of small-volume samples, as it provides sharp peaks even with small injection volume.: Diethylaminoethyl 1: Diethylaminoethyl21 1,20 5 10 15 20min1324230 5 10 15 20min4HOHOOC1. endo GA14CH3HOHOOCSample : 100μL1. Conalbumin 0.1%2. Transferrin 0.2%3. Ovalbumin 0.2%4. Trypsin inhibitor 0.2%Gibberellin Isomers2. GA14340 5 10 15 20minColumn : Shodex IEC DEAE-825Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0), 20mM Bis-Tris-HCl buffer(pH7.0)20mM Tris-HCl buffer(pH8.0), 20mM Ethanolamine-HCl buffer(pH9.0)20mM 1,3-Diaminopropane-HCl buffer(pH10.0)(B); (A) + 0.5M NaClLinear gradient; (A) to (B), 20minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 25˚Cnon-porous gel DEAE3N-4TpH7.0pH9.0Sample :1. Conalbumin 0.4mg/mL2. Ovalbumin 1.2mg/mL3. Trypsin inhibitor 1.0mg/mLpH10.0CH3COOHCOOH12Sample :GibberellinStandard AnalysisColumnsColumns for Anion Exchange Chromatography0 5 10 15 20 25 30 min0 5 minColumnEluentFlow rateDetectorColumn temp. : Room temp.Injection vol. : 100μL: Shodex IEC DEAE-825: (A); 20mM Piperazine-HCl buffer(pH6.0)(B); (A) + 0.5M NaClLinear gradient; (A) to (B), 60min: 1.0mL/min: UV(280nm)ColumnEluentFlow rateDetectorColumn temp. : Room temp.Injection vol. : 20μL: Shodex IEC DEAE3N-4T: (A); 25mM Piperazine-HCl buffer(pH6.0)(B); A + 0.5M NaClLinear gradient; (A) to (B), 10min: 1.5mL/min: UV(280nm)0 10 20 minColumn : Shodex Asahipak ES-502N 7CEluent : Acetic acid/H 2 O/CH 3 OH=0.1/0.4/99.5Flow rate : 1.5mL/minDetector : UV(210nm)Column temp. : 50˚CData was provided by Prof. Yamaguch,Faculty of Agliculture, University of Tokyo.Mercaptoalbumin andnon-mercaptoalbuminNucleotidesSulfide ion and cyanide ion1 23Sample : Normal control serum1. Globulin2. Uric acid3. HMA(Human Mercaptoalbumin)4. HNA(Human Nonmercaptoalbumin)12467Sample : 20μL1. 5'CMP 20μg/mL2. 5'UMP 20μg/mL3. 5'TMP 20μg/mL4. 3'CMP 20μg/mL5. 5'ADP 100μg/mL6. 3'UMP 20μg/mL7. 5'GMP 20μg/mL8. 2'AMP 20μg/mL9. 3'AMP 20μg/mL10. 3'GMP 20μg/mL89Sample : 20μL1. HS - 0.1mg/L2. CN - 0.02mg/L12435100.0 1.5 3.0 4.5 6.0 7.5 9.0 10.5 12.0 13.5 15.0 16.5 18.0min0 816 24 minColumn : Shodex Asahipak ES-502N 7CEluent : 50mM N-methylpiperazine-HClbuffer(pH4.8) + 400mM Na 2 SO 4 aq.+ 0.3% C 2 H 5 OHFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 35˚C0 5 10 15 20 25 30minColumn : Shodex AXpak WA-624Eluent : 0.35M CH 3 COOH aq./0.35M CH 3 COONH 4 aq.=240/100Flow rate : 1.0mL/minDetector : UV(260nm)Column temp. : 60˚CColumn : Shodex IEC DEAE-825Eluent : 10mM Na 2 CO 3+ 1mM Ethylenediamine aq.+ 10% CH 3 OHFlow rate : 1.0mL/minDetector : Electrochemical(Electrode; Silver, 0mV SCE)Column temp. : 25˚C53

Standard AnalysisColumnsColumns for Cation Exchange ChromatographyFeaturesSP-825, CM-825Suitable for the analysis of relatively high molecular weight compounds,such as proteins, peptides, DNA, and RNAUsable under wide pH range between 2 and 12NotebookNo.7Semi-microMicro ColumnsPreparativeColumnsp.77p.81Columns for Cation Exchange ChromatographySP-420NSP-2BES-502C 7CP-421SNon-porous base materialFor rapid analysisNon-porous base materialSupports UHPLC (available under hyperbaric conditions for up to 30 MPa)NotebookNotebookNo.6No.7NewsNo.42Compared to IEC series columns, polyvinyl alcohol is used as base material andthis offers different separation patternLow hydrophobic interaction with proteins allows analysis under mild conditionsColumn for amino acids analysis by cation exchange modeSupports simultaneous analysis of different amino acidsNotebookNo.3Semi-microMicro ColumnsPreparativeColumnsp.77p.81Standard columnsStrong cation exchange resin (Functional Group : Sulfopropyl)ProductCodeProduct NameIon ExchangeCapacity(meq/g)Base MaterialParticleSize(µm)PoreSize(Å)Column Size(mm)I.D. x LengthShipping SolventF6118250IEC SP-8250.4Polyhydroxymethacrylate85,0008.0 × 7550mM Na 2 SO 4 aq.F6113000IEC SP-420N0.3Polyhydroxymethacrylate2.5−4.6 × 3520mM Sodium acetate buffer+ 0.5M Na 2 SO 4 (pH5.0)F6113110PIKESS SP-2B0.3Polyhydroxymethacrylate2.5−2.0 × 5020mM Sodium acetate buffer+ 0.5M Na 2 SO 4 (pH5.0)Weak cation exchange resin (Functional Group : Carboxymethyl)ProductCodeProduct NameIon ExchangeCapacity(meq/g)Base MaterialParticleSize(µm)PoreSize(Å)Column Size(mm)I.D. x LengthShipping SolventF6110002IEC CM-8250.4Polyhydroxymethacrylate85,0008.0 × 7550mM Na 2 SO 4 aq.F7640001Asahipak ES-502C 7C0.55Polyvinyl alcohol92,0007.5 × 1000.1M Sodium phosphate buffer(pH4.4)For amino acids (Functional Group : Sulfo (Na + ))ProductCodeProduct NamePlate Number(TP/column)Base MaterialParticleSize(µm)Column Size(mm)I.D. x LengthShipping SolventF6354211CXpak P-421S≥ 3,500Styrene divinylbenzene copolymer64.6 × 150H 2 OF6700210CXpak P-G(guard column)Styrene divinylbenzene copolymer64.6 × 10H 2 O54*Contact Shodex or our distributors near you for customized columns.

Protein separation using cation exchange columns( I ) CM-825(Weak cation exchange)90µL injected10 15 30 45 min123423546HOHO1243560 15 30 min 0 2 4 6 minColumn : ( I ) Shodex IEC CM-825, ( II ) IEC SP-825, ( III ) IEC SP-420NEluent : (A); 20mM Sodium phosphate buffer(pH7.0) (B); (A) + 0.5M NaCl( I , II ) Linear gradient; (A) to (B), 60min ( III ) Linear gradient; (A) to (B), 10minFlow rate : ( I , II )1.0mL/min ( III )1.5mL/minDetector : UV(280nm)Column temp. : Room temp.Sample : 300μg/mL each, 10μLCH 2·CH·NH 2COOH2. AdrenalineHO CH·CH 2·NHHO OH CH 33. NoradrenalineHO CH·CH 2·NH 2HOOH12123456Sample :1. Myoglobin2. Trypsinogen3. Ribonuclease A4. α-Chymotrypsinogen A5. Cytochrome c6. LysozymeRapid analysis of proteins using UHPLCCatecholamines Angiotensins Paraquat and diquat1. DOPA( II ) SP-825(Strong cation exchange)30µL injected( III ) SP-420N(Strong cation exchange)non-porous type gelSample : 10μL1. Angiotensin II (human)Asp-Arg-Val-Tyr-lle-His-Pro-Phe2. Angiotensin I (human)Asp-Arg-Val-Tyr-lle-His-Pro-Phe-His-Leu3. Angiotensin III (human)Arg-Val-Tyr-lle-His-Pro-Phe10.02345Sample : 5μL (13mg total protein )1. Myoglobin2. Trypsinogen3. Ribonuclease A4. α-Chymotrypsinogen A5. Cytochrome c6. Lysozyme61.0 2.0 3.0 4.0minColumn : Shodex PIKESS SP-2BEluent : (A); 20mM Sodium phosphatebuffer(pH 7.0)(B); (A) + 0.5 M NaClLinear gradient; 100%(A) to 50%(B),2.5minFlow rate : 1.2mL/minDetector : UV(280nm)Column temp. : 25˚CParaquatSample : 20μL+ +H 3 C N N CH 3 2Cl - Diquat+ 2Br -N + NStandard AnalysisColumnsColumns for Cation Exchange Chromatography4. DopamineHOHOCH 2·CH 2·NH 230 20 min0 10 20 30 min0 10 20 minColumn : Shodex Asahipak ES-502C 7CEluent : 20mM Sodium malonate buffer+ 0.5M NaCl(pH6.0)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 30˚CColumn : Shodex Asahipak ES-502C 7CEluent : 50mM Sodium malonate buffer(pH6.0)/CH 3 CN=80/20Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : 30˚CColumn : Shodex Asahipak ES-502C 7CEluent : 50mM Sodium phosphate buffer+ 150mM NaCl(pH7.0)Flow rate : 1.0mL/minDetector : UV(288nm)Column temp. : 30˚CAnalysis of pochonicine and their analogues in filamentousfungi culture extractStandard amino acidsSample : 5μLFraction purified from the culture extract ofPochonia suchlasporia var. suchlasporia TAMA 87(Fungi culture provided by Dr. Toru Okuda,Tamagawa University Research Institute)CysSample : 2.5nmol each, 100μLThrSerGluAspGlyValMetIleLysAlaHisPochonicineLueTyrPheNH3ArgAnalogue AAnalogue BPro0 5 10 15 20 25 30 35 minColumn : Shodex Asahipak ES-502C 7CEluent : 10mM Ammonium bicarbonate aq.Flow rate : 0.6mL/minDetector : UV(210nm)Column temp. : 40˚CData provided by Dr. Teruhiko Nitoda, Faculty of Agriculture, Okayama University.01020 30 40 50 minColumn : Shodex CXpak P-421SEluent : No.1 0.12M Sodium citrate buffer(pH3.3)No.2 0.13M Sodium citrate buffer(pH3.2)No.3 0.11M Sodium citrate buffer(pH4.0)No.4 1.02M Sodium citrate buffer(pH4.9)No.5 0.2M NaOH aq./Ethanol=90/10 (Rinse solution)Low pressure gradient;No.1 (0min), No.2 (1.2min), No.3 (10.0min), No.4 (21.2min), No.5 (40min)Flow rate : 0.5mL/minDetector : VIS(570nm)Post column method(Ninhydrin reaction 0.35mL/min, 120˚C)Column temp. : 63˚C55

Standard AnalysisColumnsColumns for Special Separation ModesColumn for Hydrophobic Interaction ChromatographyPH-814FeaturesSeparates proteins without denaturationApplicable to samples obtained after ammonium sulfate fraction treatmentStandard columnColumns for Chiral Separation Columns for High Temperature Reversed Phase Chromatography[Columns for Special Separation Modes] Column for Hydrophobic Interaction Chromatography Columns for Affinity ChromatographyProductCodeProduct NameColumn Size (mm)I.D. x LengthF6110003 HIC PH-814 Phenyl10 2,000 8.0 × 75H 2OProductCodeProductCodeProduct NameProduct NameFunctional GroupParticle Size(µm)Ligand Load/Gel(g)Pore Size(Å)Columns for Affinity ChromatographyAFpakFeaturesStandard columnsF7118954F7118945F7118946F7113050F7118959F7118964Columns for Chiral SeparationFeaturesCDBS-453CRX-853AFpak ADS-894AFpak AHR-894AFpak APA-894AFpak APG-894AFpak AWG-894AFpak ACH-494Standard columnsF7146003F7140040F6709300Rigid polymer-based packing materials enable high speed analysisFunctional group is modified with chemically stable ligand (spacer)Minimum detachment of functional groups ensures highly repeatable analysisORpak CDBS-453ORpak CRX-853ORpak CRX-G(guard column)LigandDextran sulfateHeparinProtein AProtein GWheat germ agglutinin(WGA)Choline oxydase,Acetylcholine esterase30mg5mg4mg4–5mg14mg–Particle Size(µm)ParticleSize(µm)Column Size (mm)I.D. x LengthColumn Size(mm)I.D. x LengthShipping SolventColumns for High Temperature Reversed Phase Chromatography1818181818188.0 × 508.0 × 508.0 × 508.0 × 508.0 × 504.6 × 10Shipping Solvent50mM Sodium phosphate buffer+ 0.02% NaN 3 (pH7.4)10mM Tris-HCl buffer + 10mM NaCl+ 0.02% NaN 3 (pH7.4)0.1M Sodium phosphate buffer+ 0.5M NaCl + 0.02% NaN 3(pH7.0)10mM Sodium phosphate buffer+ 0.15M NaCl + 0.02% NaN 3 (pH7.4)0.1M Tris-HCl buffer + 0.15M NaCl+ 0.2M N-Acetylglucosamine+ 0.02% NaN 3 (pH7.4)10mM Phosphate buffer+ 1.0M NaCl(pH7.4)Separates optical isomers by using their conformational compatibility differencesVersatile column for chiral separationFunctional Groupβ-Cyclodextrin derivativeL-amino acid derivativeL-amino acid derivativeBase MaterialSilicaPolyhydroxymethacrylatePolyhydroxymethacrylate3664.6 × 1508.0 × 504.6 × 10Base Material : PolyhydroxymethacrylateBase Material : PolyhydroxymethacrylateSeparates optical isomers by using the differences in metal complex formation capacities offunctional group and metal ion in eluent and optical isomersSuitable for amino acids, hydroxyl acids, and their derivativesFeaturesET-RP1 Capable of high temperature analysis up to 150˚CHigh temperature analysis improves column efficiency and enables rapid analysisShipping Solvent1.0% Acetic acid + 0.2M NaCl aq./CH 3 CN=70/300.25mM CuSO 4 aq.0.25mM CuSO 4 aq.NewsNo.41Standard columnsProductCodeProduct NamePlate Number(TP/column)Functional GroupParticle Size(µm)Pore Size(Å)Column Size (mm)I.D. x LengthShipping Solvent56F7623001F7623003ET-RP1 4DET-RP1 3D≥ 11,000≥ 9,000OctadecylOctadecyl442502504.6 × 1503.0 × 150H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65Base Material : Polyvinyl alcohol*Contact Shodex or our distributors near you for customized columns.

Protein separation by hydrophobicinteraction chromatography01HO23EpinephrinesSample : 370μL1. Cytochrome c 0.03%2. Myoglobin 0.08%3. Ribonuclease A 0.16%4. Ovalbumin 0.16%5. Lysozyme 0.04%6. α-Chymotrypsinogen50.05%6420 40 60 minColumn : Shodex HIC PH-814Eluent : (A); 1.8M Ammonium sulfate + (B)(B); 0.1M Phosphate buffer(pH7.0)Linear gradient; (A) to (B), 60minFlow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Sample : Epinephrine 50μg/mL, 50μLOHNHOHCH 3121230 510 minColumn : Shodex RSpak DE-413Post column : Shodex AFpak ACH-494Eluent : 0.1M H 3 PO 4 +300mg/L Sodium 1-decansulfonate +65mg/L Tetramethylammonium chloride(pH8.0 adjusted by 1.0M NaOH)Flow rate : 1.0mL/minDetector : Electrochemical(Electrode : Pt, 350mV SCE)Column temp. : 37˚C0 5 10 15 20 25 0 5 10 15 20 25 30 35 0 5 10 15minminminColumn : Shodex ORpak CDBS-453Eluent : 0.05% Acetic acid + 0.2M NaCl aq./CH 3 CN=95/5Flow rate : 0.5mL/minDetector : UV(254nm)Column temp. : 10˚CMandelic acidsSample : Mandelic acid 100μg/mL, 20μL1. D-Mandelic acid2. L-Mandelic acid0 5 10 15 20 25minCholine and acetylcholineWarfarinSample : Warfarin 20μg/mL, 20μL2 3OOH1 40 1 2 3minOSample : 10μL1. Choline 5mg/L2. Ethylhomocholine 10mg/L3. Acetylcholine 10mg/LAfter choline and acetylcholineare separated using DE-413polymer-based reversedphase column, solutes arepassed through ACH-494 togenerate hydrogen peroxide.The resulting hydrogenperoxide is detected using anelectrochemical detector toenable highly sensitiveanalysis.CHCH2COCH3Column : Shodex ORpak CDBS-453Eluent : 1.0% Acetic acid + 0.2M NaCl aq./CH 3 CN=80/20Flow rate : 0.6mL/minDetector : UV(310nm)Column temp. : 16˚C0* See page 10 for DE-41312Lipoproteins in plasmaLDLEluent A Eluent BHDLEluent A30 10 20 30 min 0 10 20 30 minEluent BSample :Lipoproteins(Sigma)2 4 6 8 10 12 14min1Ligand : Dextran sulfate0 10 20 30 40 minColumn : Shodex AFpak ADS-894Eluent : (A); 50mM Sodium phosphate buffer(pH7.4)(B); (A) + 1.0M NaClStep gradient; (A) to (B)Flow rate : 1.0mL/minDetector : UV(280nm)Column temp. : Room temp.Lactic acidsSample : Lactic acid 50μg/mL, 50μL1. L-Lactic acid2. D-Lactic acidColumn : Shodex ORpak CRX-853Eluent : 0.5mM CuSO 4 aq.Flow rate : 1.0mL/minDetector : UV(230nm)Column temp. : 50˚CSample :1. Uracil2. Pyridine3. Acetophenone4. BenzeneSampleVLDLEluent A2Reduced plate height *40˚CAcetophenoneBenzene3.23.62.42.3* Plate height / particle diameter of the packed resin4Eluent BComparison of ET-RP1’s column efficiencies (theoretical plate height) observed athigh and normal temperature conditionshigh temp. (150˚C)2.4mL/minnormal temp. (40˚C)0.5mL/min150˚CStandard AnalysisColumnsColumns for Chiral Separation Columns for High Temperature Reversed Phase Chromatography[Columns for Special Separation Modes] Column for Hydrophobic Interaction Chromatography Columns for Affinity ChromatographyColumn : Shodex ORpak CRX-853Eluent : 0.25mM CuSO 4 aq.Flow rate : 1.0mL/minDetector : UV(230nm)Column temp. : 50˚CColumn : Shodex ET-RP1 4DEluent : (Left) H 2O/CH 3CN=50/50(Right) H 2O/CH 3CN=75/25Detector : Photodiode array(210nm)Column Oven : Polaratherm 9000 Series(SandraSelerity Technonogies, Inc)Note :The eluent was introduced into the columnafter being preheated and was cooled aftercolumn elution, then introduced into thedetector.Data provided by Research Institute for Chromatography bvba57

Standard AnalysisColumnsColumns for LC/MS AnalysisColumns for Polymer-based Reversed Phase ChromatographyProductCodeProduct NamePlate Number(TP/column)FunctionalGroupBase MaterialParticleSize(µm)PoreSize(Å)Column Size(mm)I.D. x LengthShipping SolventF7622004ODP2 HP-2B≥ 3,000−Polyhydroxymethacrylate5402.0 × 50H 2O/CH 3CN=55/45F7622005ODP2 HP-2D≥ 7,000−Polyhydroxymethacrylate5402.0 × 150H 2 O/CH 3 CN=55/45Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC) Aqueous/Organic SEC Columns Organic SEC (GPC) Columns[Columns for LC/MS Analysis] Columns for Polymer-based Reversed Phase ChromatographyF6714011F7620009F6713001F7001007F6700151F7008160F7008220ODP2 HPG-2AAsahipak ODP-50 2DAsahipak ODP-50G 2ARSpak DE-213RSpak DE-SGRSpak NN-414RSpak JJ-50 2D(guard column)≥ 5,000(guard column)≥ 8,000(guard column)≥ 6,000≥ 3,500−OctadecylOctadecyl−−SulfoAqueous/Organic SEC ColumnsPolyhydroxymethacrylatePolyvinyl alcoholPolyvinyl alcoholPolymethacrylatePolymethacrylatePolyhydroxymethacrylatePolyvinyl alcohol55546105−250−25−2001002.0 × 102.0 × 1502.0 × 102.0 × 1502.0 × 104.6 × 1502.0 × 150H 2 O/CH 3 CN=55/45H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=35/65H 2 O/CH 3 CN=50/50H 2 O/CH 3 CN=50/500.1M Sodium phosphatebuffer(pH3.0)H 2 O/CH 3 CN=40/60Columns for Polymer-based Hydrophilic Interaction Chromatography(HILIC)ProductCodeF7630008F7630009F7630010F7630006F6713000Asahipak NH2P-40 2BAsahipak NH2P-40 2DAsahipak NH2P-40 2EAsahipak NH2P-50 2DAsahipak NH2P-50G 2AQuaternaryammonium* Asahipak NH2P-40 2B, Asahipak NH2P-40 2D, and Asahipak NH2P-40 2E are the new names for the NH2P40-2B, NH2P40-2D, and NH2P40-2E, respectively.ProductCodeF7600100F7600110F7600024F7600120Organic SEC (GPC) ColumnsProductCodeF6028111Product NameProduct NameMSpak GF-310 4BMSpak GF-310 4DMSpak GF-310 4EMSpak GF-310 2DProduct NameGPC KF-401HQPlate Number(TP/column)≥ 2,000≥ 5,500≥ 7,000≥ 3,500(guard column)Plate Number(TP/column)≥ 3,000≥ 10,000≥ 16,000≥ 5,500PlateNumber(TP/column)≥ 25,000FunctionalGroupAminoAminoAminoAminoAminoExclusion Limit(Pullulan)1,50040,00040,00040,00040,000ExclusionLimit(Polystyrene)Base MaterialPolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholBase MaterialPolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholPolyvinyl alcoholBase MaterialParticleSize(µm)Styrene divinylbenzene copolymer44455ParticleSize(µm)5555PoreSize(Å)3100100100100−ParticleSize(µm)Column Size(mm)I.D. x Length2.0 × 502.0 × 1502.0 × 2502.0 × 1502.0 × 10MaximumPore Size(Å)400400400400MaximumPore Size(Å)50Shipping SolventCH 3 CNCH 3 CNCH 3 CNH 2 O/CH 3 CN=25/75H 2 O/CH 3 CN=25/75Column Size(mm)I.D. x Length4.6 × 504.6 × 1504.6 × 2502.0 × 150Column Size(mm)I.D. x Length4.6 × 250ShippingSolventH 2 OH 2 OH 2 OH 2 OShippingSolventTHFF6028112GPC KF-402HQ≥ 25,0005,000Styrene divinylbenzene copolymer31504.6 × 250THFF6028114GPC KF-402.5HQ≥ 25,00020,000Styrene divinylbenzene copolymer33004.6 × 250THF58F6700300GPC KF-G(guard column)−Styrene divinylbenzene copolymer8−4.6 × 10THF*Contact Shodex or our distributors near you for customized columns.

Barbital recovery rate comparison of ODP2 HP-2B and ODS in the presence of BSAODP2 HPBarbital 500ng/mLBarbitalPeak Area : 100%ODSBarbital 500ng/mLBarbitalPeak Area : 100%For the LC/MS analysis of drug samplescontaining protein as a matrix, using ODScolumns generally exhibit ion suppressionof the target drugs. Since proteins are notretained by ODP2 HP column, they eluteseparate (as a void) from the targetcomponents. Thus, using ODP2 HP columnexhibits less ion suppression influence.Standard AnalysisColumns0.0 1.0 2.0 3.0 4.0 min 0.00 2 4 6 8 100 1into MS2Barbital 500ng/mL in BSA 7.0mg/mL1BarbitalPeak Area : 99%LC/MS analysis of drugs in control serumSample : 5μLDrugs 500ng/mL each in Control serumUV(280m)12 minLC/MS analysis of basic drugs0Lidocaine2 4 6 8 10 12 14 min3 4 5 6 7 8 9 min21.0 2.0 3.0 4.0 minm/z 235Chlorpheniraminem/z 275VerapamilPropranololm/z 260Diphenhydraminem/z 256BupivacaineImipramineColumn : Shodex ODP2 HP-2BEluent : 10mM Ammonium acetate aq./CH 3 CN=70/30Flow rate : 0.2mL/minDetector : UV(280nm), ESI-MS(SIM Positive)Column temp. : 40˚Cm/z 304 m/z 290m/z 289m/z 455m/z 281ODP-50 2D, a polymer-based reversed phase column, is capable of analyzingbasic drugs at low concentration or without salt. This is preferred for ESI-MSanalysis, since the presence of salt causes ion suppression, and thus influencesthe analysis performance.Column : Shodex Asahipak ODP-50 2DEluent : H 2 O/CH 3 CN=40/60Flow rate : 0.2mL/minDetector : ESI-MS(SIM Positive)Column temp. : 30˚CSample : 10ng/mL each, 5μL1. Scopolamine2. AtropineBarbital 500ng/mL in BSA 7.0mg/mLBarbitalPeak Area : 71%LC/MS analysis of statinsPravastatin0 1 2 3 4 5 6 7 8 9Glyoxylic acidPyruvic acidGlycolic acidLactic acidPitavastatinFluvastatinAtorvastatinMevastatin10 11 12 13 14 15 16 17 18 19 20 21 minMaleic acidLovastatinSuccinic acidFumaric acidSimvastatinLC/MS analysis of organic acidsColumn : Shodex ODP2 HP-2BODS from other manufacturerEluent : 10mM Ammonium acetate aq./CH 3 CN=70/30Flow rate : 0.2mL/minDetector : ESI-MS(SIM Negative : m/z 183)Column temp. : 30˚CInjection vol. : 10μLAdipic acid0 1 2 3 4 5 6 7 8 9 minSample : 100ng/mL each, 5μLm/z 423m/z 422m/z 412m/z 559m/z 391m/z 405m/z 419Column : Shodex ODP2 HP-2BEluent : (A); 10mM Ammonium formate aq. (B); CH 3 CNLinear gradient;B%; 10%(0min) 10%(3min) 35%(13min) 35%(20min)Flow rate : 0.2mL/minDetector : ESI-MS(SIM)Column temp. : 40˚CSample : 50ng/mL each, 10μLm/z 73m/z 87m/z 75m/z 89m/z 115m/z 117m/z 115m/z 145Column : Shodex RSpak DE-213Eluent : (A); 0.1%(v/v) Formic acid aq. (B); CH 3 CNLinear gradient;B%; 5%(0min) 5%(2min) 15%(2.5min) 15%(10min)Flow rate : 0.2mL/minDetector : ESI-MS(SIM Negative)Column temp. : 30˚CColumns for Polymer-based Hydrophilic Interaction Chromatography (HILIC) Aqueous/Organic SEC Columns Organic SEC (GPC) Columns[Columns for LC/MS Analysis] Columns for Polymer-based Reversed Phase Chromatography59

Standard AnalysisColumnsLC/MS analysis of N-acetyl-chitooligosaccharidesN-Acetyl-D-glucosamine2merSample : 5μLMixture of N-acetyl-chitooligosaccharides 500ng/mL3merm/z 220m/z 423m/z 626LC/TOF-MS analysis of 2-amino benzoic acid derivatizedsugar chains1234 5Sample : 2μLHuman IgG (1μg protein)2AA-labeled N-Glycan* 2AA : 2-Aminobenzoic acidColumns for Polymer-based Hydrophilic Interaction Chromatography (HILIC) Aqueous/Organic SEC Columns Organic SEC (GPC) Columns60[Columns for LC/MS Analysis] Columns for Polymer-based Reversed Phase Chromatography012NCH3NHCreatinineNH3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 min412114merH2N5mer36mer0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 minSample : 100ng/mL each, 5μL1. Creatinine2. Creatine1O10CH3NNHCreatineLC/MS/MS analysis of estradiol metabolites5OOH26m/z 829m/z 1032m/z 1235Column : Shodex Asahipak NH2P-40 2DEluent : (A); 0.05% Ammonia aq. (B); CH 3 CNLinear gradient; (B%) 75%(0min) 50%(10min) 50%(15min)Flow rate : 0.2mL/minDetector : ESI-MS(SIM Negative)Column temp. : 30˚CLC/MS analysis of creatinine and creatineColumn : Shodex Asahipak NH2P-40 2DEluent : (A); 50mM Ammonium formate aq. (B); CH 3 CNLinear gradient;B%, 90%(0min) 90%(6min) 75%(7min) 75%(20min)Flow rate : 0.2mL/minDetector : Creatinine : ESI-MS, Creatine : ESI-MS/MSColumn temp. : 30˚C7895 10 15 20Migration time (min)Column : Shodex Asahipak NH2P-40 2DEluent : (A); 95% CH 3 CN/0.1% Formic acid aq.(B); 5% CH 3 CN/0.1% Formic acid aq.Linear gradient; (B%) 30%(0-2.5min), 30-95%(2.5-20min)Flow rate : 0.2mL/minDetector : ESI-TOF MS(Polarity : Negative, Full MS range : 2000 )Column temp. : 45˚CData provided by Michihiro Kinoshita Ph.D., Faculty of Pharmacy, Kinki UniversityLC/MS analysis of melamine and cyanuric acid0 5 10 15Time/minData provided by Mr. Atsushi Yamamoto,Osaka City Institute of Public Health and Environmental Sciences0 121H 2N N NH 2NNH2NMelamine3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 min2HOSample : 500ng/mL each, 5μL1. Melamine2. Cyanuric acidNNOHNCyanuric acidColumn : Shodex Asahipak NH2P-40 2DEluent : 50mM Ammonium formate aq./CH 3 CN=25/75Flow rate : 0.2mL/minDetector : Melamine : ESI-MS/MSCyanuric acid : ESI-MSColumn temp. : 30˚CSample : 0.1ppm each, 5μL1. Estrone-3-sulfate2. β-Estradiol-3-sulfate3. Estriol-3-sulfate4. Dehydroepiandrosterone-3-sulfate5. Estrone-3-glucuronide6. β-Estradiol-17-glucuronide7. β-Estradiol-3-glucuronide8. Estriol-3-glucuronide9. Estriol-16-glucuronide10. Dehydroepiandrosterone-3-glucuronide11. Androsterone-3-glucuronideColumn : Shodex Asahipak NH2P-40 2DEluent : (A); 10mM NH 4 OH CH 3 CN(B); 10mM NH 4 OH H 2 OLinear gradient;B%, 22%(0-5min), 22-70%(5-10min),70%(10-15min)Flow rate : 0.2mL/minDetector : ESI-MS/MS(Polarity : Negative)Column temp. : 40˚COH6

Speciation of arsenic2000Standard arsenic compounds110002 3 45 6Sample : Arsenic compounds, 50μL1. Monomethylarsonic acid2. Arsinic acid3. Dimethylarsinic acid4. Arsenobetaine5. Tetramethylarsonium6. Trimethylarsine oxideLC/MS analysis of perchloric acid and oxyhalidesClO 4-Sample : 5μLClO - 4 0.2μg/LClO - 3 5μg/LBrO - 3 5μg/LCl - 1mg/Lm/z 99Standard AnalysisColumns0 100 200 300 400 500 600 700 800 900 10001100 1200 secClO3 - BrO 3-m/z 832000100012340 100 200 300 400 500 600 700 800 900 10001100 1200 secColumn : Shodex RSpak NN-614Eluent : 5mM HNO 3 /8mM NH 4 NO 3 aq.Flow rate : 0.8mL/minDetector : IC-ICP-MS(SIM m/z 75)20001000231LC/MS analysis of nonylphenol ethoxylatesColumnEluentFlow rateDetectorUrine from a patient witharsenic poisoning: Shodex MSpak GF-310 4D: (A) CH 3 CN, (B): H 2 OA/B=30/70(7min) (23min) A/B=90/10: 0.5mL/min: ESI-MS(SIM Positive)Urine of a healthy person45 6100 200 300 400 500 600 700 800 900 100011001200 secSource:Noriko Tsunoda,Pharmacia. 1998, vol.34, No.12, p.1237-1241Sample : Nonylphenol ethoxylates, 20μLSource:Mr. Yoshinari Kobuke (Environmental Science Institute of Hyogo Prefecture),Basic Study of Chemical Substances in LC/MS,10th Symposium on Environmental Chemistry Program and Abstracts, 542-543, 2001.0 5 10 15 20 minLC/MS analysis of additivesSample : Cup of instant noodles (styrene foam) 1000mg/L, 5μL001 2 3 41 2 3 4Cl -m/z 127m/z 35Column : Shodex RSpak JJ-50 2DEluent : 50mM Ammonium formate aq./CH 3 CN=25/75Flow rate : 0.2mL/minDetector : ESI-MS(SIM Negative)Column temp. : 30˚CThe analysis of additives that are present in polymers usuallyrequire sample pretreatment steps. However, by using sizeexclusion chromatography columns, easy and high sensitiveanalysis of additives was achieved without any pretreatmentsteps. The target additives are separated from the polymer andthis allows introducing only the low molecular fraction (whichcontains the additives) into MS.UV(210nm)m/z 1176(Negative)5 6 7 8 9 10 11 12 13 14 15 16min5 6 7 8 9 10 11 12 13 14 15 16minColumn : Shodex GPC KF-402HQ x 2Eluent : THFFlow rate : 0.3mL/minDetector : UV(210nm), APCI-MS(SIM)Column temp. : 40˚Cnot detectedIRGANOX ® 1010Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC) Aqueous/Organic SEC Columns Organic SEC (GPC) Columns[Columns for LC/MS Analysis] Columns for Polymer-based Reversed Phase Chromatography61

Sample PretreatmentColumnsColumns for GPC Clean-upFeaturesEV Suitable for fractionation of residual pesticides in foodUsed in the purification process of test solution preparation method in Shoku-An No. 1003001 (October 3rd,2006. Japan) of the Pharmaceutical and Food Safety Bureau, MHLW, Section 2 "Simultaneous GC/MS(LC/MS) Analyses of Agricultural Chemicals in Livestock and Marine Products"Columns for GPC Clean-upPAESuitable for cleaning up high-moisture samples such as blood and bottom sedimentHighly effective for fractionation of endocrine disruptors in environmental samplesAppropriate columnsfor GPC Clean-upDoes the samplecontain water?NoYesEluentEthylacetate/CyclohexaneAcetone/CyclohexaneAcetonitrileAcetoneColumnEV-2000, EV-200EV-2000 AC, EV2000AC-12FPAE-2000, PAE-800PAE-2000 AC, PAE-800 ACGPC Clean-up for residual pesticides in food, etc.Product CodeProduct NameParticle Size(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LengthShipping SolventF6090006CLNpak EV2000AC-12F163012.0 × 300Acetone/Cyclohexane=3/7F6090007CLNpak EV-G AC12C16(guard column)12.0 × 100Acetone/Cyclohexane=3/7F6090003CLNpak EV-2000 AC163020.0 × 300Acetone/Cyclohexane=3/7F6090004CLNpak EV-G AC16(guard column)20.0 × 100Acetone/Cyclohexane=3/7F6090005CLNpak EV-20016302.0 × 150Ethylacetate/Cyclohexane=3/7F6090001CLNpak EV-2000163020.0 × 300Ethylacetate/Cyclohexane=3/7F6090002CLNpak EV-G16(guard column)20.0 × 100Ethylacetate/Cyclohexane=3/7Base Material : Styrene divinylbenzene copolymerGPC Clean-up for phthalic acid esters in sediments, biological samples, blood, etc.Product CodeProduct NameParticle Size(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LengthShipping SolventF7600025CLNpak PAE-80054008.0 × 300AcetonitrileF6810022CLNpak PAE-2000540020.0 × 300AcetonitrileF6714007CLNpak PAE-G9(guard column)8.0 × 50AcetonitrileF7600026CLNpak PAE-800 AC54008.0 × 300AcetoneF6810023CLNpak PAE-2000 AC540020.0 × 300AcetoneF6714008CLNpak PAE-G AC9(guard column)8.0 × 50AcetoneBase Material : Polyvinyl alcohol62*Contact Shodex or our distributors near you for customized columns.

Sample preparation outline for simultaneous GC/MS and LC/MS analysis ofagricultural chemicals in livestock and marine productsExtractionLiver, KidneyAcetone/n-Hexane (1/2)GPCMuscle, Fat, Fish Milk, Egg HoneyGPCAcetonitrileSalting-outGPC Clean-up of residual pesticides using EV-2000 ACAcrinathrin (5mg/L)(marker pesticide)Tricyclazole (5mg/L)(marker pesticide)Sample PretreatmentColumnsPurificationFraction-1[*1] [*1] [*1][*2]Fraction-2PesticidefractionTest sampleGC/MS, LC/MSPesticidefraction*1 Purification with ethylenediamine-N-propylsilyled silica gel mini-column*2 Purification with silica gel mini-columnGPC Clean-up of fatty tuna and beef tallowmuscle, fat,fish, milk, eggliver, kidneyfraction-1pesticidefractionfraction-2Column : Shodex CLNpak EV-G AC + EV-2000 ACEluent : Acetone/Cyclohexane=1/4Flow rate : 5.0mL/minDetector : UV(254nm)Column temp. : 40˚CInjection vol. : 5mLPCB and insulating oil using PAE-800Columns for GPC Clean-up0Sample : 5mLBeef tallowFatty tuna1. Acrinathrin 5mg/L2. Tricyclazole 5mg/LGPC Clean-up of beef tallowGPC Clean-up of fatty tuna1 25 10 15 20 25 30 35 minColumn : Shodex CLNpak EV-G AC + EV-2000 ACEluent : Acetone/Cyclohexane=1/4Flow rate : 5.0mL/minDetector : UV(254nm)Column temp. : 40˚CInjection vol. : 5mL[Extraction]1) Weigh 20.0g of the sample (5.0g for fat).2) Add 20mL of water and homogenize.3) Add 100mL of acetone/n-hexane (1/2)and homogenize.4) Centrifuge (2,500rpm, 5min.) and collectthe organic layer (1).5) Add 50mL of n-hexane to the residueand homogenize.6) Centrifuge (2,500rpm, 5min.) and collectthe organic layer (2).7) Combine (1) and (2) and add anhydroussodium sulfate for dehydration.8) Remove anhydrous sodium sulfate byfiltering and concentrate the filtrate at40˚C or lower.9) Remove the solvent.10) Weigh the residue (extracted fat).11) Dissolve in acetone/cyclohexane (1/4).Sample : 30μLPCB std. KC-mixtureInsulating oildissolved by hexaneMethanolAcetoneHexane400mg/L5,000mg/LInsulating oilPCB0 10 20 30Retention time (min)Column : Shodex CLNpak PAE-800Eluent : Methanol, Acetone, HexaneFlow rate : 0.8mL/minDetector : Photodiode array(209nm)Column temp. : 40˚CSource:Mr. Tetsuya Sawatsubashi (Mitsubishi Heavy Industries, Ltd.) etal., Search of Liquid Chromatographic Clean-up Materials forRapid PCB Analysis and Evaluation of Their SeparationCharacteristics. Journal of Environmental Chemistry, 2007,Vol. 17, No. 3, p. 471-481.Pretreatment of PCBs in waste oilGPC Clean-up of carcinogens in dieseldust measured using PAE-800 ACElution positions of phthalic acid estersusing PAE-800 ACPCBsBenzo[a]fluoranthene1,3-DinitropyreneBisphenol ABenzo[a]pyreneHydroxybenzo[a]pyrene mixtureRI Corn oil SulferH 2 OCO 2Time 14 15 16 17 18 19 20 minVolume 56 60 64 68 72 76 80 mLAliphaticC 19Chlordane,hydrocarbonC 12PCBsToxaphenePolychlorinatedPolybrominatedMethylanthracenenaphthalenebiphenyletherAnthraceneColumn : Shodex CLNpak PAE-G AC+ PAE-2000 ACEluent : AcetoneFlow rate : 4.0mL/minDetector : UV(213nm)Data provided by Mr. Koichi Sasaki et al.,Analytical Center, Kokan Keisoku Corporation.10 20 30minCompound Rt(min) Compound Rt(min)AnthraceneChryseneFluorantheneBenzo[a]flucrantheneBenzo[b]flucrantheneBenzo[k]flucranthenePyrene1-Hydroxypyrene16.618.017.117.718.618.417.919.7Benzo[a]pyreneHydroxybenzo[a]pyrenecompoundBisphenol A1,3-Dinitronaphthalene1-Nitropyrene1,3-Dinitropyrene1,6-Dinitropyrene1,8-Dinitropyrene19.923.016.116.218.618.618.918.7Column : Shodex CLNpak PAE-800 ACEluent : AcetoneFlow rate : 0.8mL/minDetector : UV(210nm)Column temp. : Room temp.Data provided by Kazuichi Hayakawa Ph.D.,Faculty of Pharmaceutical Sciences, Kanazawa University.RIRIRIRIUV(330nm)UV(330nm)Bis(2-ethylhexyl) adipatePhthalic acid esters4-NonylphenolDDTBisphenol AAnthracene0 15 20 25 30 35minColumn : Shodex CLNpak PAE-800 ACEluent : AcetoneFlow rate : 0.5mL/minDetector : UV(330nm), RIColumn temp. : Room temp.63

Sample PretreatmentColumnsPretreatment Columns for Column SwitchingMethodPKFeaturesEffective for both hydrophilic and hydrophobic substancesThe high protein removal rate enables efficient pretreatmentNewsNo.9, 11, 22Pretreatment Columns for Column Switching MethodGF-4AHigher protein removal rate than PK columns* GF-4A column removes proteins well but is not suitable for trapping hydrophilic substances.Use PK columns for this purpose.NewsNo.9, 11, 22Cartridge columns and holder for column switching methodProduct CodeF8700000F8700012F8700001Product NameMSpak PK-2A 2pMSpak PK-4A 2pMSpak HLDColumn for column switching methodParticle Size(µm)3030−Pore Size(Å)3030−Column Size(mm)I.D. x Length2.0 x 104.0 x 10(Holder for PK)Shipping SolventH 2 OH 2 O−Pcs/boxBase Material : Hydrophilic copolymers containing N-vinyl acetoamide* PK series are cartridge columns and thus should be installed in a column holder "MSpak HLD" before use.221Product CodeProduct NameParticle Size(µm)Pore Size(Å)Column Size(mm)I.D. x LengthShipping SolventF8700015 MSpak GF-4A 9 400 4.6 × 10H 2 OBase Material : Polyvinyl alcohol64*Contact Shodex or our distributors near you for customized columns.

System diagram for column switchingPretreatment columnAnalytical columnInjectorDetectorPump 1Protein removal rate using pretreatment columnsColumnPK-2AGF-2A*Company-AProtein removalrate99.62%99.80%97.68%Residual BSA (μg)500400300200100Sample PretreatmentColumnsEluent 1Proteins etc.in sampleRecovery rate of medical compounds using PK-2ASampleAcetaminophenAcetylpheneturideAconitineAlprazolamAmitriptylineAmobarbitalBarbitalBenzoylaconitineBenzoylhypaconineBiperidenBromazepamBromocriptineBromperidolBromvalerylureaBrotizolamCaffeineCarbamazepineCarpipramineChlordiazepoxideChlormezanoneChlorpheniramineChlorpromazineClocapramineClofedanolClomipramineClonazepamPump 2Recovery(%)1159211099939394968399102808994971069799133921117795919596Eluent 2SampleCloxazolamDesipramineDiazepamDiphenhydramineEstazolamEthenzamideEtizolamFludiazepamFlumazenilFlunitrazepamFlurazepamGlutethimideHaloperidolHaloxazolam1-Hydroxymethyltriazolam4-HydroxytriazolamHydroxyzineHypaconitineImipramineIndomethacinLevomepromazineLofepramineMaprotylineMedazepamMephobarbitalMesaconine0PK-2A(Pretreatment)Eluent : 10mM Ammonium acetate buffer(pH7.0)Flow rate : 0.5mL/minColumn temp. : Room temp.GF-2A Company-A(Analysis)Column : Shodex PROTEIN KW-604S**Eluent : 0.1% TFA in (H 2 O/CH 3 CN=50/50)Flow rate : 0.5mL/minDetector : UV(280nm)*GF-2A: Custom-made column ofColumn temp. : Room temp.GF-4A (2.0mm I.D. x 10mm)Switching time : 5min**KW-604S is phase-out product.Recovery(%)911099793101981059793971069399939091999797939665909199118SampleMianserinNimetazepamNitrazepamNortriptylineOxazepamOxazolamPentobarbitalPerfenazinePhenacetinPhenobarbitalPhenytoinPrimidonePromethazinePropericiazinePropranololSecobarbitalSildenafil citrateThioridazineTimiperoneTriazolamTrihexyphenidylTrimethadioneTrimipramineWarfarinZotepineRecovery(%)92909786979993861089699919290979795978896911371078192Pretreatment Columns for Column Switching MethodClotiazepam96Metharbital94(Adsorption)Eluent : 10mM Ammonium acetate buffer (pH7.0)Flow rate : 0.5mL/min(Elution)Eluent : 10mM Ammonium acetate buffer (pH7.0)/CH 3 CNFlow rate : 0.5mL/minDetector : UV(220nm)Switching time : 5min65

Application FieldsPharmaceuticals and Cosmetics AnalysisPharmaceuticals, MetabolitesEmulsifiersProduct Name Separation Mode PageProduct Name Separation Mode PageODP2 HPAsahipak ODP-40Asahipak ODP-50Asahipak C8P-50Asahipak C4P-50RSpak DS seriesRSpak DE seriesRSpak NN seriesRPCRPCRPCRPCRPCRPCRPCIEC+RPC6, 5888, 58881010, 5810, 58Asahipak GF-310 HQMSpak GF-310GPC KF-802GPC KF-402HQPreservativesSEC+RPCSEC+RPCSECSEC3434, 583642, 58Asahipak NH2P seriesODSpak F-411Silica C18MSilica C18PUSPpak MN-431ET-RP1HILICRPCRPCRPCLEX+SECRPC12, 581414141656Product NameAsahipak ODP-40Asahipak ODP-50RSpak DS seriesRSpak DE seriesODSpak F-411Separation ModeRPCRPCRPCRPCRPCPage88, 581010, 5814[Substances in bio-fluid]Silica C18MRPC14Silica C18PRPC14Product Name Separation Mode PageODP2 HPRPCAsahipak GS-320 HQ SEC+RPCAsahipak GF-310 HQ SEC+RPCMSpak GF-310SEC+RPCMSpak PKSEC+RPCMoisturizers[Polyalcohols]Pharmaceuticals & CosmeticsColumn List for Pharmaceuticals and Cosmetics Analysis6, 583234Optical active materials34, 5864Product Name Separation Mode PageORpak CDBS-453ORpak CRX-853CSCS5656Product Name Separation Mode PageRSpak DE seriesSUGAR SC1011SUGAR SC1211OHpak SB-802.5 HQAsahipak GF-310 HQMSpak GF-310RPCLEX+SECLEX+HILICSECSECSEC10, 581616303434, 58[Protein hydrolysates]Product Name Separation Mode PageAsahipak ODP-40Asahipak ODP-50RSpak RP18-415PROTEIN KW-802.5KW402.5RPCRPCRPCSECSEC88, 58102828[Mucopolysaccharides]Product Name Separation Mode PageOHpak SB-800 HQ series SEC30Separation ModeRPC : Reversed Phase ChromatographyHILIC : Hydrophilic Interaction ChromatographyNPC : Normal Phase ChromatographyLEX : Ligand Exchange ChromatographyIEX : Ion Exclusion ChromatographyIC : Ion ChromatographySEC : Size Exclusion ChromatographyIEC : Ion Exchange ChromatographyHIC : Hydrophobic Interaction ChromatographyAFC : Affinity ChromatographyCS : Chiral Separation Chromatography66

FoodNutritional ingredientsColumn List for Food Analysis[Monosaccharides, Disaccharides, Sugar alcohols] [Fatty acids]Product Name Separation Mode PageProduct Name Separation Mode PageAsahipak NH2P seriesSUGAR SC1011SUGAR SC1821SUGAR SP0810SUGAR KS-801SUGAR KS-802SUGAR SC1211SUGAR SZ5532RSpak DC-613HILICLEX+SECLEX+SECLEX+SECLEX+SECLEX+SECLEX+HILICLEX+HILICLEX+HILIC12, 581616161616161616RSpak RP18 seriesRSpak DE seriesSilica C18MSilica C18PSilica 5SILAsahipak GF-310 HQMSpak GF-310GPC KF-802GPC KF-402 HQRPCRPCRPCRPCRPCSECSECSECSEC1010, 581414143434, 583642, 58[Oligosaccharides][“Umami”, Nucleic acids]Application Fields Food AnalysisProduct Name Separation Mode PageAsahipak NH2P series HILIC 12, 58SUGAR KS-802SEC16RSpak DC-613LEX+HILIC 16OHpak SB-802 HQSEC30OHpak SB-802.5 HQSEC30Asahipak GS-220 HQSEC32[Low molecular weight water-soluble fiber]Product Name Separation Mode PageAsahipak GS-220 HQSEC32[Polysaccharides]Product Name Separation Mode PageSUGAR KS-800 seriesSEC16OHpak SB-800 HQ series SEC30[Organic acids]Product Name Separation Mode PageODP2 HPRPC6, 58RSpak DE seriesRPC10, 58RSpak NN seriesIEX+RPC 10, 58RSpak KC-811IEX+RPC 20SUGAR SH1011IEX+RPC 20SUGAR SH1821IEX+RPC 20IC SI-90 4EIC22IC SI-50 4EIC22IC SI-35 4DIC22IC SI-52 4EIC22[Water-soluble vitamins]Product Name Separation Mode PageODP2 HPRPC6, 58Asahipak ODP-40RPC8Asahipak ODP-50RPC8, 58RSpak DE seriesRPC10, 58RSpak NN seriesIEC+RPC 10, 58Asahipak NH2P series HILIC 12, 58[Fat-soluble vitamins]Product Name Separation Mode PageAsahipak ODP-40Asahipak ODP-50Silica 5SILGPC KF-801GPC KF-401HQRPCRPCNPCSECSEC88, 58143642, 58Product Name Separation Mode PageAsahipak GS-320 HQ IEC+SEC 32[Amino acids]Product Name Separation Mode PageRSpak NN seriesIC YS-50CXpak P-421SIEC+IEX+RPCICIEC10, 582454Food safety[Food additives]Product Name Separation Mode PageAsahipak ODP-40Asahipak ODP-50RSpak DS seriesRSpak DE seriesAsahipak NH2P seriesET-RP1RPCRPCRPCRPCHILICRPC88, 581010, 5812, 5856[Pesticides]Product Name Separation Mode PageRSpak DE seriesRSpak NN seriesIC SI-90 4ERPCIEC+RPCIC10, 5810, 5822[Mycotoxins]Product Name Separation Mode PageSilica C18M 4ERPC14[Pretreatment of residual pesticides]Product Name Separation Mode PageCLNpak EV seriesSEC(GPC Clean-up) 62Separation ModeRPC : Reversed Phase ChromatographyHILIC : Hydrophilic Interaction ChromatographyNPC : Normal Phase ChromatographyLEX : Ligand Exchange ChromatographyIEX : Ion Exclusion ChromatographyIC : Ion ChromatographySEC : Size Exclusion ChromatographyIEC : Ion Exchange ChromatographyHIC : Hydrophobic Interaction ChromatographyAFC : Affinity ChromatographyCS : Chiral Separation Chromatography67

Application FieldsBiotechnology AnalysisColumn List for Biotechnology AnalysisGenomicsProteomicsBiotechnology[Nucleobases, Nucleotides, Nucleosides][Amino acids]Product Name Separation Mode PageProduct Name Separation Mode PageRSpak DE seriesRSpak NN seriesAsahipak GS-320 HQAXpak WA-624RPCIEC+RPCIEC+SECIEC10, 5810, 583252Asahipak ODP-50RSpak NN seriesIC YS-50CXpak P-421SRPCIEC+IEX+RPCIECIEC8, 5810, 582454Asahipak GS-320 HQ IEC+SEC 32[Oligo nucleic acids]Product Name Separation Mode Page[Peptides, Proteins]RSpak DE seriesRPC10, 58Product Name Separation Mode PageAsahipak GS-320 HQIEC DEAE3N-4TAsahipak ES-502N 7CIEC+SECIECIEC325252[DNA, RNA]Product Name Separation Mode PageOHpak SB-800 HQ series SEC30Asahipak GF-HQ series SEC34Hormones[Amines]Product Name Separation Mode PageODP2 HPAsahipak ODP-40Asahipak ODP-50Asahipak C8P-50Asahipak C4P-50RSpak DS seriesRSpak DE seriesAsahipak ES-502C 7CRPCRPCRPCRPCRPCRPCRPCIEC6, 5888, 58881010, 5854Asahipak ODP-40Asahipak ODP-50Asahipak C8P-50Asahipak C4P-50RSpak RP18-415PROTEIN KW-800 seriesKW400 seriesOHpak SB-800 HQ seriesAsahipak GS-HQ seriesAsahipak GF-HQ seriesIEC QA-825IEC DEAE-825IEC DEAE3N-4TPIKESS DEAE-2BAsahipak ES-502N 7CIEC SP-825IEC SP-420NPIKESS SP-2BIEC CM-825Asahipak ES-502C 7CHIC PH-814RPCRPCRPCRPCRPCSECSECSECSECSECIECIECIECIECIECIECIECIECIECIECHIC88, 58881028283032345252525252545454545456[Steroids]Product Name Separation Mode PageAsahipak ODP-40Asahipak ODP-50Asahipak NH2P seriesOHpak SB-802.5 HQAsahipak GF-310 HQMSpak GF-310RPCRPCHILICSECSECSEC88, 5812, 58303434, 58Separation ModeRPC : Reversed Phase ChromatographyHILIC : Hydrophilic Interaction ChromatographyNPC : Normal Phase ChromatographyLEX : Ligand Exchange ChromatographyIEX : Ion Exclusion ChromatographyIC : Ion ChromatographySEC : Size Exclusion ChromatographyIEC : Ion Exchange ChromatographyHIC : Hydrophobic Interaction ChromatographyAFC : Affinity ChromatographyCS : Chiral Separation Chromatography68

BiotechnologyColumn List for Biotechnology AnalysisGlycomicsLipids[Glycoproteins]Product Name Separation Mode Page[Phospholipids]Product Name Separation Mode PageAsahipak ODP-40Asahipak ODP-50Asahipak C8P-50Asahipak C4P-50RSpak RP18-415RPCRPCRPCRPCRPC88, 588810Silica 5SILAsahipak GF-310 HQMSpak GF-310GPC KF-802GPC KF-402HQNPCSECSECSECSEC143434, 583642PROTEIN KW-800 seriesKW400 seriesOHpak SB-800 HQ seriesSECSECSEC282830[Lipoproteins]Product Name Separation Mode PageAsahipak GS-HQ seriesAsahipak GF-HQ seriesIEC QA-825IEC DEAE-825IEC DEAE3N-4TPIKESS DEAE-2BAsahipak ES-502N 7CIEC SP-825IEC SP-420NPIKESS SP-2BIEC CM-825Asahipak ES-502C 7CHIC PH-814AFpak AWG-894SECSECIECIECIECIECIECIECIECIECIECIECHICAFC3234525252525254545454545656OHpak SB-805 HQAFpak ADS-894AFpak AHR-894SECAFCAFC305656Application FieldsBiotechnology Analysis[Sugar chains]Product Name Separation Mode PageAsahipak NH2P seriesAFpak AWG-894HILICAFC12, 5856[Monosaccharides, Disaccharides, Sugar alcohols]Product Name Separation Mode PageAsahipak NH2P seriesSUGAR SC1011SUGAR SC1821SUGAR SP0810SUGAR KS-801SUGAR KS-802SUGAR SC1211SUGAR SZ5532RSpak DC-613HILICLEX+SECLEX+SECLEX+SECLEX+SECLEX+SECLEX+HILICLEX+HILICLEX+HILIC12, 581616161616161616[Sialic acids, Uronic acids, Aldonic acids]Product Name Separation Mode PageSUGAR SH1011IEX+SEC 20SUGAR SH1821IEX+SEC 20Separation ModeRPC : Reversed Phase ChromatographyHILIC : Hydrophilic Interaction ChromatographyNPC : Normal Phase ChromatographyLEX : Ligand Exchange ChromatographyIEX : Ion Exclusion ChromatographyIC : Ion ChromatographySEC : Size Exclusion ChromatographyIEC : Ion Exchange ChromatographyHIC : Hydrophobic Interaction ChromatographyAFC : Affinity ChromatographyCS : Chiral Separation Chromatography69

Application FieldsWater quality[Anions]Column List for Environmental AnalysisProduct Name Separation Mode PageIC NI-424IC I-524AIC SI seriesICICIC222222EnvironmentEnvironmental hormonesPretreatment of Phthalates, PCBs,Benzo[a]pyreneProduct Name Separation Mode PageCLNpak PAE seriesSEC(GPC Clean-up)62Environmental Analysis[Oxyhalides]Product Name Separation Mode PageIC SI-35 4DIC22IC SI-52 4EIC22RSpak JJ-50 2DIEC58[Cyanide, Cyanogen chloride]Product Name Separation Mode PageRSpak KC-811 6EIEX20[Cations]Product Name Separation Mode PageIC YS-50IC24IC YK-421IC24IC Y-521IC24[Surfactants]Product Name Separation Mode PageAsahipak ODP-50 4DSilica C18MAsahipak GF-310 HQMSpak GF-310RPCRPCSEC+RPCSEC+RPC8143434, 58Bioethanol[Monosaccharides, Oligosaccharides]Product Name Separation Mode PageAsahipak NH2P seriesSUGAR SP0810HILICLEX+SEC12, 5816[Oligosaccharides, Alcohols, Furfural]Product Name Separation Mode PageSUGAR KS-802SUGAR SC1821LEX+SECLEX+SEC1616[Saccharides, Organic acids, Alcohols, Furfural]Product Name Separation Mode PageSUGAR SH1011SUGAR SH1821IEX+RPC+SECIEX+RPC+SEC2020[Hemicellulose, Cellulose]Product Name Separation Mode PageGPC KD-800 seriesGPC LF-804SECSEC404470[Perchloric acids]Product Name Separation Mode PageSI-90 4EIC22RSpak JJ-50 2DIEC58[Pesticides]Product Name Separation Mode PageRSpak DE seriesRSpak NN seriesIC SI-90 4ERPCIEC+RPCIC10, 5810, 5822Soil[Anions]Product Name Separation Mode PageIC NI-424IC I-524AIC SI seriesICICIC222222[Heavy metals]Product Name Separation Mode PageIC T-521IC24[Humic substances]Product Name Separation Mode PageOHpak SB-805 HQSEC30[Organic arsenics]Product Name Separation Mode PageRSpak NN seriesIEC+RPC 10, 58[Pesticides]Product Name Separation Mode PageRSpak DE seriesRPC10, 58RSpak NN seriesIEC+RPC 10, 58IC SI-90 4EIC22Biodiesel[Cations]Product Name Separation Mode PageIC YS-50IC24[Fatty acid glycerides]Product Name Separation Mode PageAsahipak GF-210 HQAsahipak GF-310 HQMSpak GF-310SECSECSEC343434, 58[Fatty acid methyl esters]Product Name Separation Mode PageRSpak DS seriesRPC10[Organic acids]Product Name Separation Mode PageIC SI-35 4DIC22IC SI-52 4EIC22Separation ModeRPC : Reversed Phase ChromatographyHILIC : Hydrophilic Interaction ChromatographyNPC : Normal Phase ChromatographyLEX : Ligand Exchange ChromatographyIEX : Ion Exclusion ChromatographyIC : Ion ChromatographySEC : Size Exclusion ChromatographyIEC : Ion Exchange ChromatographyHIC : Hydrophobic Interaction ChromatographyAFC : Affinity ChromatographyCS : Chiral Separation Chromatography

USP (Ver.35 US Pharmacopeia) Column ListNo. Packing Material Recommended Column PageL1L3L7L8L10L11L17L19L20L21L22L23Octadecyl silane chemically bonded to porous or non-porous silica or ceramicmicro-particles, 1.5 to 10μm in diameter, or a monolithic rodPorous silica particles, 1.5 to 10μm in diameter, or a monolithic silica roOctylsilane chemically bonded to totally or superficially porous silica particles, 1.5 to 10μmin diameter, or a monolithic silica rodAn essentially monomolecular layer of aminopropylsilane chemically bonded to totallyporous silica gel support, 1.5 to 10μm in diameterNitrile groups chemically bonded to porous silica particles, 1.5 to 10μm in diameterPhenyl groups chemically bonded to porous silica particles, 1.5 to 10μm in diameterStrong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzenecopolymer in the hydrogen form, 6 to 12μm in diameterStrong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzenecopolymer in the calcium form, about 9μm in diameterDihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to10μm in diameterA rigid, spherical styrene-divinylbenzene copolymer, 3 to 30μm in diameterA cation-exchange resin made of porous polystyrene gel with sulfonic acid groups,about 10μm in sizeAn anion-exchange resin made of porous polymethacrylate or polyacrylate gel withquartenary ammonium groups, 7-12μm in sizeSilica C18MSilica C18PODSpak F-411Silica 5SILSilica 5C8Silica 5NHSilica 5CNSilica 5NPESUGAR SH1011SUGAR SH1821RSpak KC-811IC Y-521SUGAR SC1011SUGAR SC1821SUGAR SC1211USPpak MN-431PROTEIN KW-800 seriesKW400 seriesGPC KF,K,KD,HFIP,LF,AT,UT seriesRSpak DS-613RSpak DS-413RSpak RP18-415CXpak P-421SSUGAR SP0810SUGAR SC1011SUGAR SC1821SUGAR KS-800 seriesSUGAR SC1211SUGAR SZ5532USPpak MN-431RSpak DC-613SUGAR SH1011SUGAR SH1821RSpak KC-811IC Y-521IEC QA-82514141414141414142020202416161616282836, 38, 40, 42,44, 46, 481010105416161616161616162020202452Application fields USP (Ver.35) Column ListL25Packing having the capacity to separate compounds with a molecular weight range from100-5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationicwater-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylatedether (surface contained some residual carboxyl functional groups) was found suitableOHpak SB-802 HQOHpak SB-802.5 HQ3030L26L33Butyl silane chemically bonded to totally porous silica particles, 1.5 to 10μm in diameterPacking having the capacity to separate dextrans by molecular size over a range of 4,000to 500,000Da. It is spherical, silica-based, and processed to provide pH stabilitySilica 5C4PROTEIN KW-800 seriesKW400 series142828L34Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzenecopolymer in the lead form, about 7 to 9μm in diameterSUGAR SP081016L37Packing having the capacity to separate proteins by molecular size over a range of 2,000to 40,000 Da. It is a polymethacrylate gelOHpak SB-803 HQ30L38L39A methacrylate-based size-exclusion packing for water-soluble samplesA hydrophilic polyhydroxymethacrylate gel of totally porous spherical resinOHpak SB-800 HQ seriesOHpak SB-800 HQ seriesODP2 HPRSpak DM-61430306, 5810L45L58Beta cyclodextrin, R,S-hydroxypropyl ether derivative, bonded to porous silica particles,5 to 10μm in diameterStrong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzenecopolymer in the sodium form, about 6 to 30μm diameterORpak CDBS-453CXpak P-421SSUGAR KS-800 seriesRSpak DC-61356541616L59Packing for the size-exclusion separations of proteins (separation by molecular weight)over the range of 5 to 7000kDa. It is spherical (1.5 - 10μm), silica or hybrid packing witha hydrophilic coating.PROTEIN KW-800 seriesKW400 series2828L67L71Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group ofthe polymer, 2 to 10μm in diameterA rigid, spherical polymetacrylate, 4 to 6μm in diameterAsahipak ODP-40Asahipak ODP-50ET-RP1RSpak DE-613RSpak DE-413RSpak DE-21388, 5856101010, 5871

Semi-micro andMicro ColumnsSemi-microMicro ColumnsPolymer-based Packed Columns for Reversed Phase and Hydrophilic Interaction Chromatography (HILIC)Semi-microMicro ColumnsPolymer-based Packed Columns for Reversed Phase and Hydrophilic Interaction Chromatography (HILIC)I.D.2.0mm1.0mm0.5mmLength(mm)250150503525015050352501505035Product NameODP40-2EODP40-2DODP40-2BODP40-2TODP40-1EODP40-1DODP40-1BODP40-1TODP40-M5EODP40-M5DODP40-M5BODP40-M5T* See page 8 for Asahipak ODP-40.* See page 78 for preparative columns.I.D.2.0mm1.0mm0.5mmLength(mm)150503515050351505035* See page 10 for RSpak NN-414.Semi-micro and Micro ColumnsAsahipak ODP-40 semi-micro and micro typeBase Material : Polyvinyl alcoholFunctional Group : OctadecylNN414-2DNN414-2BNN414-2TNN414-1DNN414-1BNN414-1TNN414-M5DNN414-M5BNN414-M5TProduct CodeF7838021F7838022F7838023F7838024F7838031F7838032F7838033F7838034F7838051F7838052F7838053F7838054RSpak NN-414 semi-micro and micro typeBase Material : PolyhydroxymethacrylateFunctional Group : SulfoProduct NameProduct CodeF7860122F7860123F7860124F7860132F7860133F7860134F7860152F7860153F7860154RSpak DE-413 semi-micro and micro typeBase Material : PolymethacrylateLengthI.D.(mm)Product Name2.0mm1.0mm0.5mm250150503525015050351505035DE413-2EDE413-2DDE413-2BDE413-2TDE413-1EDE413-1DDE413-1BDE413-1TDE413-M5DDE413-M5BDE413-M5T* See page 10 for RSpak DE-413 series.* See page 78 for preparative columns.I.D.2.0mm1.0mm0.5mmLength(mm)250150503525015050352501505035Product NameNH2P40-2ENH2P40-2DNH2P40-2BNH2P40-2TNH2P40-1ENH2P40-1DNH2P40-1BNH2P40-1TNH2P40-M5ENH2P40-M5DNH2P40-M5BNH2P40-M5TProduct CodeF7840121F7840122F7840123F7840124F7840131F7840132F7840133F7840134F7840152F7840153F7840154Asahipak NH2P-40 semi-micro and micro typeBase Material : Polyvinyl alcoholFunctional Group : AminoProduct CodeName ChengeRefer to p.58F7858024F7858031F7858032F7858033F7858034F7858051F7858052F7858053F7858054* See page 12 for Asahipak NH2P-40 series.* See page 79 for preparative columns.72*Contact Shodex or our distributors near you for customized columns.

KW402.5 semi-micro and micro typeBase Material : SilicaI.D.4.6mm2.0mm1.0mm0.5mmLength(mm)15050250150502501505025015050Product NameKW402.5-4DKW402.5-4BKW402.5-2EKW402.5-2DKW402.5-2BKW402.5-1EKW402.5-1DKW402.5-1BKW402.5-M5EKW402.5-M5DKW402.5-M5B* See page 28 for KW402.5-4F.* See page 81 for preparative columns.Product CodeF7781212F7781213F7781221F7781222F7781223F7781231F7781232F7781233F7781251F7781252F7781253Semi-micro and Micro ColumnsAqueous SEC (GFC) Column : Silica-basedKW403 semi-micro and micro typeBase Material : SilicaI.D.4.6mm2.0mm1.0mm0.5mmLength(mm)15050250150502501505025015050Product NameKW403-4DKW403-4BKW403-2EKW403-2DKW403-2BKW403-1EKW403-1DKW403-1BKW403-M5EKW403-M5DKW403-M5B* See page 28 for KW403-4F.* See page 81 for preparative columns.Semi-microMicro ColumnsProduct CodeF7781312F7781313F7781321F7781322F7781323F7781331F7781332F7781333F7781351F7781352F7781353Semi-micro andMicro ColumnsSemi-microMicro ColumnsAqueous SEC (GFC) Column : Silica-basedKW404 semi-micro and micro typeBase Material : SilicaI.D.4.6mmLength(mm)15050Product NameKW404-4DKW404-4BProduct CodeF7781412F7781413KW405 semi-micro and micro typeBase Material : SilicaI.D.4.6mmLength(mm)15050Product NameKW405-4DKW405-4BProduct CodeF7781512F7781513250KW404-2EF7781421250KW405-2EF77815212.0mm150KW404-2DF77814222.0mm150KW405-2DF778152250KW404-2BF778142350KW405-2BF7781523250KW404-1EF7781431250KW405-1EF77815311.0mm150KW404-1DF77814321.0mm150KW405-1DF778153250KW404-1BF778143350KW405-1BF7781533250KW404-M5EF7781451250KW405-M5EF77815510.5mm150KW404-M5DF77814520.5mm150KW405-M5DF778155250KW404-M5BF778145350KW405-M5BF7781553* See page 28 for KW404-4F.* See page 81 for preparative columns.* See page 28 for KW405-4F.* See page 81 for preparative columns.73*Contact Shodex or our distributors near you for customized columns.

Semi-micro andMicro ColumnsSemi-microMicro ColumnsAqueous SEC (GFC) Columns : Polymer-basedSemi-microMicro ColumnsSemi-micro and Micro ColumnsLength(mm)25015050250150502501505025015050Length(mm)25015050250150502501505025015050Aqueous SEC (GFC) Columns : Polymer-basedOHpak SB-802 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateI.D.4.6mm2.0mm1.0mm0.5mmProduct NameSB802-4ESB802-4DSB802-4BSB802-2ESB802-2DSB802-2BSB802-1ESB802-1DSB802-1BSB802-M5ESB802-M5DSB802-M5B* See page 30 for OHpak SB-802 HQ.* See page 81 for preparative columns.Product NameSB803-4ESB803-4DSB803-4BSB803-2ESB803-2DSB803-2BSB803-1ESB803-1DSB803-1BSB803-M5ESB803-M5DSB803-M5BProduct CodeF7770111F7770112F7770113F7770121F7770122F7770123F7770131F7770132F7770133F7770151F7770152F7770153OHpak SB-803 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateI.D.4.6mm2.0mm1.0mm0.5mmProduct CodeF7770311F7770312F7770313F7770321F7770322F7770323F7770331F7770332F7770333F7770351F7770352F7770353OHpak SB-802.5 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateI.D.4.6mm2.0mm1.0mm0.5mmLength(mm)25015050250150502501505025015050* See page 30 for OHpak SB-802.5 HQ.* See page 81 for preparative columns.Length(mm)25015050250150502501505025015050Product NameSB802.5-4ESB802.5-4DSB802.5-4BSB802.5-2ESB802.5-2DSB802.5-2BSB802.5-1ESB802.5-1DSB802.5-1BSB802.5-M5ESB802.5-M5DSB802.5-M5BProduct NameSB804-4ESB804-4DSB804-4BSB804-2ESB804-2DSB804-2BSB804-1ESB804-1DSB804-1BSB804-M5ESB804-M5DSB804-M5BProduct CodeF7770211F7770212F7770213F7770221F7770222F7770223F7770231F7770232F7770233F7770251F7770252F7770253OHpak SB-804 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateI.D.4.6mm2.0mm1.0mm0.5mmProduct CodeF7770411F7770412F7770413F7770421F7770422F7770423F7770431F7770432F7770433F7770451F7770452F7770453* See page 30 for OHpak SB-803 HQ.* See page 81 for preparative columns.* See page 30 for OHpak SB-804 HQ.* See page 81 for preparative columns.74OHpak SB-805 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateI.D.4.6mm2.0mm1.0mm0.5mmLength(mm)25015050250150502501505025015050Product NameSB805-4ESB805-4DSB805-4BSB805-2ESB805-2DSB805-2BSB805-1ESB805-1DSB805-1BSB805-M5ESB805-M5DSB805-M5B* See page 30 for OHpak SB-805 HQ.* See page 81 for preparative columns.Product CodeF7770511F7770512F7770513F7770521F7770522F7770523F7770531F7770532F7770533F7770551F7770552F7770553OHpak SB-806 HQ semi-micro and micro typeBase Material : PolyhydroxymethacrylateI.D.4.6mm2.0mm1.0mm0.5mmLength(mm)25015050250150502501505025015050Product NameSB806-4ESB806-4DSB806-4BSB806-2ESB806-2DSB806-2BSB806-1ESB806-1DSB806-1BSB806-M5ESB806-M5DSB806-M5B* See page 30 for OHpak SB-806 HQ.* See page 81 for preparative columns.Product CodeF7770611F7770612F7770613F7770621F7770622F7770623F7770631F7770632F7770633F7770651F7770652F7770653*Contact Shodex or our distributors near you for customized columns.

Asahipak GS-220 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.Length(mm)250Product NameGS220A-4EMultimode ColumnsProduct CodeF7750211Semi-micro and Micro ColumnsI.D.Length(mm)250Product NameGS320A-4ESemi-microMicro ColumnsAsahipak GS-320 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholProduct CodeF7750311Semi-micro andMicro ColumnsSemi-microMicro Columns4.6mm2.0mm1505025015050250GS220A-4DGS220A-4BGS220A-2EGS220A-2DGS220A-2BGS220A-1EF7750212F7750213F7750221F7750222F7750223F77502314.6mm2.0mm1505025015050250GS320A-4DGS320A-4BGS320A-2EGS320A-2DGS320A-2BGS320A-1EF7750312F7750313F7750321F7750322F7750323F7750331Multimode Columns1.0mm150GS220A-1DF77502321.0mm150GS320A-1DF775033250GS220A-1BF775023350GS320A-1BF7750333250GS220A-M5EF7750251250GS320A-M5EF77503510.5mm150GS220A-M5DF77502520.5mm150GS320A-M5DF775035250GS220A-M5BF775025350GS320A-M5BF7750353* See page 32 for Asahipak GS-220 HQ.* See page 82 for preparative columns.* See page 32 for Asahipak GS-320 HQ.* See page 82 for preparative columns.Asahipak GS-520 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.Length(mm)250Product NameGS520A-4EProduct CodeF7750511Asahipak GS-620 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.Length(mm)250Product NameGS620A-4EProduct CodeF77506114.6mm150GS520A-4DF77505124.6mm150GS620A-4DF775061250GS520A-4BF775051350GS620A-4BF7750613250GS520A-2EF7750521250GS620A-2EF77506212.0mm150GS520A-2DF77505222.0mm150GS620A-2DF775062250GS520A-2BF775052350GS620A-2BF7750623250GS520A-1EF7750531250GS620A-1EF77506311.0mm150GS520A-1DF77505321.0mm150GS620A-1DF775063250GS520A-1BF775053350GS620A-1BF7750633250GS520A-M5EF7750551250GS620A-M5EF77506510.5mm150GS520A-M5DF77505520.5mm150GS620A-M5DF775065250GS520A-M5BF775055350GS620A-M5BF7750653* See page 32 for Asahipak GS-520 HQ.* See page 82 for preparative columns.* See page 32 for Asahipak GS-620 HQ.* See page 82 for preparative columns.75*Contact Shodex or our distributors near you for customized columns.

Semi-micro andMicro ColumnsSemi-microMicro ColumnsSemi-microMicro ColumnsI.D.Semi-micro and Micro ColumnsAsahipak GF-310 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholLength(mm)250Product NameGF310A-4EAqueous/Organic SEC ColumnsProduct CodeF7760311Asahipak GF-510 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.Length(mm)250Product NameGF510A-4EProduct CodeF7760511Aqueous/Organic SEC Columns4.6mm2.0mm1.0mm150502501505025015050250GF310A-4DGF310A-4BGF310A-2EGF310A-2DGF310A-2BGF310A-1EGF310A-1DGF310A-1BGF310A-M5EF7760312F7760313F7760321F7760322F7760323F7760331F7760332F7760333F77603514.6mm2.0mm1.0mm150502501505025015050250GF510A-4DGF510A-4BGF510A-2EGF510A-2DGF510A-2BGF510A-1EGF510A-1DGF510A-1BGF510A-M5EF7760512F7760513F7760521F7760522F7760523F7760531F7760532F7760533F77605510.5mm150GF310A-M5DF77603520.5mm150GF510A-M5DF776055250GF310A-M5BF776035350GF510A-M5BF7760553* See page 34 for Asahipak GF-310 HQ.* See page 82 for preparative columns.* See page 34 for Asahipak GF-510 HQ.* See page 82 for preparative columns.Asahipak GF-710 HQ semi-micro and micro typeBase Material : Polyvinyl alcoholI.D.Length(mm)250Product NameGF710A-4EProduct CodeF77607114.6mm150GF710A-4DF776071250GF710A-4BF7760713250GF710A-2EF77607212.0mm150GF710A-2DF776072250GF710A-2BF7760723250GF710A-1EF77607311.0mm150GF710A-1DF776073250GF710A-1BF7760733250GF710A-M5EF77607510.5mm150GF710A-M5DF776075250GF710A-M5BF7760753* See page 34 for Asahipak GF-710 HQ.* See page 82 for preparative columns.76*Contact Shodex or our distributors near you for customized columns.

Columns for Anion Exchange ChromatographyIEC QA-825 semi-micro and micro typeBase Material : PolyhydroxymethacrylateFunctional Group : Quaternary ammoniumI.D.Length(mm)150Product NameQA8-2DProduct CodeF7940322Semi-micro and Micro ColumnsColumns for Cation Exchange ChromatographyIEC SP-825 semi-micro and micro typeBase Material : PolyhydroxymethacrylateFunctional Group : SulfopropylI.D.Length(mm)150Product NameSP8-2DSemi-microMicro ColumnsProduct CodeF7940122Semi-micro andMicro ColumnsSemi-microMicro Columns2.0mm1.0mm0.5mm503515050351505035QA8-2BQA8-2TQA8-1DQA8-1BQA8-1TQA8-M5DQA8-M5BQA8-M5T* See page 52 for IEC QA-825.* See page 80 for preparative columns.Length(mm)150503515050351505035Product NameDEAE8-2DDEAE8-2BDEAE8-2TDEAE8-1DDEAE8-1BDEAE8-1TDEAE8-M5DDEAE8-M5BDEAE8-M5T* See page 52 for IEC DEAE-825.* See page 80 for preparative columns.Length(mm)150Product NameDEAE9A-2DF7940323F7940324F7940332F7940333F7940334F7940352F7940353F7940354IEC DEAE-825 semi-micro and micro typeBase Material : PolyhydroxymethacrylateFunctional Group : DiethylaminoethylI.D.2.0mm1.0mm0.5mmProduct CodeF7940422F7940423F7940424F7940432F7940433F7940434F7940452F7940453F7940454Asahipak ES-502N semi-micro and micro typeBase Material : Polyvinyl alcoholFunctional Group : DiethylaminoethylI.D.Product CodeF79601222.0mm1.0mm0.5mm503515050351505035SP8-2BSP8-2TSP8-1DSP8-1BSP8-1TSP8-M5DSP8-M5BSP8-M5T* See page 54 for IEC SP-825.* See page 81 for preparative columns.Length(mm)150503515050351505035Product NameCM8-2DCM8-2BCM8-2TCM8-1DCM8-1BCM8-1TCM8-M5DCM8-M5BCM8-M5T* See page 54 for IEC CM-825.* See page 81 for preparative columns.CM9A-2DF7940123F7940124F7940132F7940133F7940134F7940152F7940153F7940154IEC CM-825 semi-micro and micro typeBase Material : PolyhydroxymethacrylateFunctional Group : CarboxymethylI.D.2.0mm1.0mm0.5mmLength(mm)150Product NameProduct CodeF7940222F7940223F7940224F7940232F7940233F7940234F7940252F7940253F7940254Asahipak ES-502C semi-micro and micro typeBase Material : Polyvinyl alcoholFunctional Group : CarboxymethylI.D.Product CodeF7960222Columns for Anion Exchange Chromatography Columns for Cation Exchange Chromatography2.0mm50DEAE9A-2BF79601232.0mm50CM9A-2BF796022335DEAE9A-2TF796012435CM9A-2TF7960224150DEAE9A-1DF7960132150CM9A-1DF79602321.0mm50DEAE9A-1BF79601331.0mm50CM9A-1BF796023335DEAE9A-1TF796013435CM9A-1TF7960234150DEAE9A-M5DF7960152150CM9A-M5DF79602520.5mm50DEAE9A-M5BF79601530.5mm50CM9A-M5BF796025335DEAE9A-M5TF796015435CM9A-M5TF7960254* See page 52 for Asahipak ES-502N 7C.* See page 80 for preparative columns.* See page 54 for Asahipak ES-502C 7C.* See page 81 for preparative columns.*Contact Shodex or our distributors near you for customized columns.77

PreparativeColumnsPreparativeColumnsPreparative Columns (I.D. 10mm~50mm)Columns for Polymer-based Reversed Phase ChromatographyAsahipak ODP-50 preparative typePreparativeColumnsProduct CodeProduct NamePlate Number(TP/column)Functional GroupParticle Size(µm)Column Size (mm)I.D. x LengthF6820001Asahipak ODP-50 10E≥ 10,000Octadecyl510.0 × 250Columns for Polymer-based Reversed Phase ChromatographyF6820035F6820019F6710004F6714029Asahipak ODP-90 20FAsahipak ODP-130 28FAsahipak ODP-130G 7BAsahipak ODP-130G 20C* See page 8 for Asahipak ODP-50.* See page 72 for semi-micro columns and micro columns.Asahipak C8P-50 preparative typeProduct CodeProduct CodeProduct NameF6820003 Asahipak C8P-50 10EF6714004 Asahipak C8P-50G 7B* See page 8 for Asahipak C8P-50.Asahipak C4P-50 preparative typeProduct NameF6820005 Asahipak C4P-50 10EF6714005 Asahipak C4P-50G 7B* See page 8 for Asahipak C4P-50.RSpak DE-613 preparative type≥ 9,000≥ 9,000(guard column)(guard column)Plate Number(TP/column)≥ 8,000(guard column)Plate Number(TP/column)≥ 7,000(guard column)OctadecylOctadecylOctadecylOctadecylFunctional GroupOctylOctylFunctional GroupButylButyl9131313Particle Size(µm)55Particle Size(µm)5520.0 × 30028.0 × 3007.5 × 5020.0 × 100Base Material : Polyvinyl alcoholColumn Size (mm)I.D. x Length10.0 × 2507.5 × 50Base Material : Polyvinyl alcoholColumn Size (mm)I.D. x Length10.0 × 2507.5 × 50Base Material : Polyvinyl alcoholProduct CodeProduct NamePlate Number(TP/column)Functional GroupParticle Size(µm)Column Size (mm)I.D. x LengthF6513013RSpak DE-2013≥ 10,000−1220.0 × 300F6700190RSpak DE-LG(guard column)−128.0 × 50F6513015RSpak DE-5013−−1250.0 × 300F6700191RSpak DE-LLG(guard column)−1220.0 × 100* See page 10 for RSpak DE-613.* See page 72 for semi-micro columns and micro columns.Base Material : PolymethacrylateRSpak DM-614 preparative typeProduct CodeProduct NamePlate Number(TP/column)Functional GroupParticle Size(µm)Column Size (mm)I.D. x LengthF6514014RSpak DM-2014≥ 5,000−1220.0 × 300F6700404RSpak DM-LG(guard column)−128.0 × 50F6514022RSpak DM-5014−−1250.0 × 300F6700162RSpak DM-LLG(guard column)−1220.0 × 100* See page 10 for RSpak DM-614.Base Material : Polyhydroxymethacrylate78*Contact Shodex or our distributors near you for customized columns.

Preparative ColumnsColumns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)PreparativeColumnsPreparativeColumnsAsahipak NH2P-50 preparative typeProduct CodeProduct NamePlate Number(TP/column)Functional GroupParticle Size(µm)Column Size (mm)I.D. x LengthPreparativeColumnsF6830001Asahipak NH2P-50 10E≥ 10,000Amino510.0 × 250F6830031F6830007F6710017Asahipak NH2P-90 20FAsahipak NH2P-130 28FAsahipak NH2P-130G 7B* See page 12 for Asahipak NH2P-50.* See page 72 for semi-micro columns and micro columns.F7560040 Silica C18M 10EF7560041 Silica C18M 20E* See page 14 for Silica C18M.F7560062 Silica 5C8 10EF7560063 Silica 5C8 20E* See page 14 for Silica 5C8.F7560054 Silica 5C4 10EF7560055 Silica 5C4 20E* See page 14 for Silica 5C4.Columns for Silica-based Reversed Phase ChromatographySilica C18M preparative typeProduct CodeProduct NameSilica 5C8 preparative typeProduct CodeProduct NameSilica 5C4 preparative typeProduct CodeProduct NameColumns for Silica-based Normal Phase and Hydrophilic Interaction Chromatography (HILIC)F7560050 Silica 5SIL 10EF7560051 Silica 5SIL 20E* See page 14 for Silica 5SIL.F7560060 Silica 5NH 10EF7560061 Silica 5NH 20E* See page 14 for Silica 5NH.≥ 10,000≥ 1,000(guard column)Plate Number(TP/column)≥ 16,000≥ 16,000Plate Number(TP/column)≥ 15,000≥ 15,000Plate Number(TP/column)≥ 15,000≥ 15,000≥ 15,000≥ 15,000≥ 8,000≥ 8,000AminoAminoAminoFunctional GroupOctadecylOctadecylFunctional GroupOctylOctylFunctional GroupButylButylSilica 5SIL preparative type [For normal phase chromatography]Product CodeProduct CodeProduct NameProduct NamePlate Number(TP/column)Plate Number(TP/column)Functional Group−−Functional GroupAminopropylAminopropyl91313Particle Size(µm)55Particle Size(µm)55Particle Size(µm)55Particle Size(µm)55Particle Size(µm)5520.0 × 30028.0 × 3007.5 × 50Base Material : Polyvinyl alcoholColumn Size (mm)I.D. x Length10.0 × 25020.0 × 250Base Material : SilicaColumn Size (mm)I.D. x Length10.0 × 25020.0 × 250Base Material : SilicaColumn Size (mm)I.D. x Length10.0 × 25020.0 × 250Base Material : SilicaColumn Size (mm)I.D. x Length10.0 × 25020.0 × 250Base Material : SilicaSilica 5NH preparative type [For normal phase and hydrophilic interaction chromatography (HILIC)]Column Size (mm)I.D. x Length10.0 × 25020.0 × 250Base Material : SilicaColumns for Polymer-based Hydrophilic Interaction Chromatography (HILIC) Columns for Silica-based Reversed Phase ChromatographyColumns for Silica-based Normal Phase and Hydrophilic Interaction Chromatography (HILIC)79*Contact Shodex or our distributors near you for customized columns.

PreparativeColumnsPreparativeColumnsPreparative ColumnsColumns for Ligand Exchange ChromatographySUGAR KS-800 series preparative typePreparativeColumnsColumns for Ligand Exchange Chromatography Columns for Ion Exclusion ChromatographyColumns for Anion Exchange ChromatographyProduct CodeF6502007F6502008F6502009F6502010F6502011F6502012F6700002Product CodeF6548000F6548050F6709602Product NameSUGAR KS-2001SUGAR KS-2002SUGAR KS-2003SUGAR KS-2004SUGAR KS-2005SUGAR KS-2006SUGAR KS-LG* See page 16 for SUGAR KS-800 series.RSpak DC-613 preparative typeProduct Code Product NameF6514013 RSpak DC-2013F6700402 RSpak DC-LGF6514021 RSpak DC-5013F6700172 RSpak DC-LLG* See page 16 for RSpak DC-613.RSpak KC-811 preparative typeProduct CodeProduct NameF6505012 RSpak KC-2011F6700010 RSpak KC-LG* See page 20 for RSpak KC-811.IEC QA-825 preparative typeProduct NameIEC QA-2025IEC QA-5025IEC QA-LG(guard column)IEC DEAE-825 preparative typePlate Number(TP/column)≥ 7,000≥ 7,000≥ 8,000≥ 6,000≥ 6,000≥ 6,000(guard column)Plate Number(TP/column)≥ 6,000(guard column)−(guard column)Columns for Ion Exclusion ChromatographyPlate Number(TP/column)≥ 8,000(guard column)Columns for Anion Exchange ChromatographyFunctional GroupQuaternary ammoniumQuaternary ammoniumQuaternary ammoniumFunctional Group(Counter Ion)Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Functional Group(Counter Ion)Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Sulfo (Na + )Functional GroupSulfoSulfoParticle Size(µm)203720Particle Size(µm)13131318181813Particle Size(µm)10101010Particle Size(µm)1313Column Size (mm)I.D. x Length20.0 × 15050.0 × 3008.0 × 50Base Material : PolyhydroxymethacrylateColumn Size (mm)I.D. x Length20.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 3008.0 × 50Column Size (mm)I.D. x Length20.0 × 3008.0 × 50Standard ColumnKS-801KS-802KS-803KS-804KS-805KS-806(guard column)Base Material : Styrene divinylbenzene copolymerColumn Size (mm)I.D. x Length20.0 × 3008.0 × 5050.0 × 30020.0 × 100Base Material : Styrene divinylbenzene copolymerBase Material : Styrene divinylbenzene copolymer* See page 52 for IEC QA-825.* See page 77 for semi-microcolumns and micro columns.Product CodeProduct NameFunctional GroupParticle Size(µm)Column Size (mm)I.D. x LengthF6548001IEC DEAE-2025Diethylaminoethyl2020.0 × 150F6598051F6709603IEC DEAE-5025IEC DEAE-LG(guard column)Asahipak ES-502N preparative typeDiethylaminoethylDiethylaminoethyl3750.0 × 300208.0 × 50Base Material : Polyhydroxymethacrylate* See page 52 for IEC DEAE-825.* See page 77 for semi-microcolumns and micro columns.Product CodeProduct NameFunctional GroupParticle Size(µm)Column Size (mm)I.D. x Length80F6840004F6710021Asahipak ES-502N 20CAsahipak GS-20G 7B(guard column)Diethylaminoethyl−132020.0 × 1007.5 × 50Base Material : Polyvinyl alcohol* See page 52 for Asahipak ES-502N 7C.* See page 77 for semi-microcolumns and micro columns.*Contact Shodex or our distributors near you for customized columns.

IEC SP-825 preparative typePreparative ColumnsColumns for Cation Exchange ChromatographyPreparativeColumnsPreparativeColumnsProduct CodeF6548002Product NameIEC SP-2025Functional GroupSulfopropylParticle Size(µm)20Column Size (mm)I.D. x Length20.0 × 150PreparativeColumnsF6548052F6709604Product CodeF6548003F6548053F6709605Product CodeF6840003F6710021IEC SP-5025IEC SP-LG(guard column)IEC CM-825 preparative typeProduct NameIEC CM-2025IEC CM-5025IEC CM-LG(guard column)Asahipak ES-502C preparative typeProduct NameAsahipak ES-502C 20CAsahipak GS-20G 7B(guard column)SulfopropylSulfopropylFunctional GroupCarboxymethylCarboxymethylCarboxymethylFunctional GroupCarboxymethylPROTEIN KW-800 series preparative typeProduct CodeProduct Name−Base Material : PolyhydroxymethacrylateParticle Size(µm)203720Column Size (mm)I.D. x Length20.0 × 15050.0 × 3008.0 × 50Base Material : PolyhydroxymethacrylateParticle Size(µm)1320Column Size (mm)I.D. x Length20.0 × 1007.5 × 50Base Material : Polyvinyl alcoholAqueous SEC (GFC) Columns : Silica-basedPlate Number(TP/column)F6505020F6505021F6505022F6709556PROTEIN KW-2002.5PROTEIN KW-2003PROTEIN KW-2004PROTEIN KW-LG≥ 17,000≥ 17,000≥ 14,000(guard column)* See page 28 for PROTEIN KW-800 series.* See page 73 for semi-micro columns and micro columns.Aqueous SEC (GFC) Columns : Polymer-based3720Particle Size(µm)557750.0 × 3008.0 × 50* See page 54 for IEC SP-825.* See page 77 for semi-microcolumns and micro columns.* See page 54 for IEC CM-825.* See page 77 for semi-microcolumns and micro columns.* See page 54 for Asahipak ES-502C 7C.* See page 77 for semi-microcolumns and micro columns.Column Size (mm)I.D. x Length20.0 × 30020.0 × 30020.0 × 3008.0 × 50Standard ColumnKW-802.5KW-803KW-804(guard column)Base Material : SilicaColumns for Cation Exchange Chromatography Aqueous SEC (GFC) Columns : Silica-basedAqueous SEC (GFC) Columns : Polymer-basedOHpak SB-800 HQ series preparative typeProduct CodeF6516011Product NameOHpak SB-2002Plate Number(TP/column)≥ 9,000Particle Size(µm)15Column Size (mm)I.D. x Length20.0 × 300Standard ColumnSB-802 HQF6516012OHpak SB-2002.5≥ 12,0001020.0 × 300SB-802.5 HQF6516013OHpak SB-2003≥ 12,0001020.0 × 300SB-803 HQF6516014OHpak SB-2004≥ 12,0001820.0 × 300SB-804 HQF6516015OHpak SB-2005≥ 12,0002020.0 × 300SB-805 HQF6516016OHpak SB-2006≥ 12,0002020.0 × 300SB-806 HQF6516017OHpak SB-2006M≥ 12,0002020.0 × 300SB-806M HQF6709555OHpak SB-LG(guard column)188.0 × 50(guard column)* See page 30 for OHpak SB-800 HQ series.* See page 74 for semi-micro columns and micro columns.Base Material : Polyhydroxymethacrylate[Note] The maximum solvent tolerance of the SB-2000 series for methanol, acetonitrile, and DMF is 50%.Solvent tolerance is different compared to the standard analytical SB-800 HQ columns.81*Contact Shodex or our distributors near you for customized columns.

PreparativeColumnsPreparativeColumnsPreparative ColumnsMultimode ColumnsPreparativeColumnsMultimode Columns Aqueous/Organic SEC Columns Organic SEC (GPC) ColumnsAsahipak GS-HQ series preparative typeProduct CodeF6810017F6810018F6810019F6810020F6810034F6810035F6810036F6810037F6710021Product CodeF6810030F6810031F6810032F6810033F6810038F6810039F6810040F6810041F6710020Product NameAsahipak GS-220 20FAsahipak GS-320 20FAsahipak GS-520 20FAsahipak GS-620 20FAsahipak GS-220 20GAsahipak GS-320 20GAsahipak GS-520 20GAsahipak GS-620 20GAsahipak GS-20G 7B* See page 32 for Asahipak GS-HQ series.* See page 75 for semi-micro columns and micro columns.Asahipak GF-HQ series preparative typeProduct NameAsahipak GS-310 20FAsahipak GS-510 20FAsahipak GS-710 20FAsahipak GSM-700 20FAsahipak GS-310 20GAsahipak GS-510 20GAsahipak GS-710 20GAsahipak GSM-700 20GAsahipak GS-10G 7BPlate Number(TP/column)≥ 8,000≥ 8,000≥ 8,000≥ 8,000≥ 14,000≥ 14,000≥ 14,000≥ 14,000(guard column)Particle Size(µm)Aqueous/Organic SEC ColumnsPlate Number(TP/column)≥ 8,000≥ 8,000≥ 8,000≥ 8,000≥ 14,000≥ 14,000≥ 14,000≥ 14,000(guard column)Particle Size(µm)131313131313131320Column Size (mm)I.D. x LengthColumn Size (mm)I.D. x Length20.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 50020.0 × 50020.0 × 50020.0 × 5007.5 × 50Standard ColumnStandard ColumnGF-310 HQGF-510 HQGF-710 HQGF-7M HQGF-310 HQGF-510 HQGF-710 HQGF-7M HQ(guard column)* See page 34 for Asahipak GF-HQ series.Base Material : Polyvinyl alcohol* See page 76 for semi-micro columns and micro columns.(Note) Usable solvents for GS-710 20F and 20G are water and methanol.Solvent tolerance is different compared to standard analytical GF-710 HQ columns.Selection of GSM-700 20F or 20G is recommended in case of use of other solvents for scale up testing with GF-710 HQ.13131313131313132020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 50020.0 × 50020.0 × 50020.0 × 5007.5 × 50GS-220 HQGS-320 HQGS-520 HQGS-620 HQGS-220 HQGS-320 HQGS-520 HQGS-620 HQ(guard column)Base Material : Polyvinyl alcoholGPC KF-800 series preparative typeOrganic SEC (GPC) ColumnsProduct CodeF6102401Product NameGPC KF-2001Plate Number(TP/column)≥ 18,000Particle Size(µm)6Column Size (mm)I.D. x Length20.0 × 300Standard ColumnKF-801F6102402GPC KF-2002≥ 18,000620.0 × 300KF-802F6102425GPC KF-2002.5≥ 18,000620.0 × 300KF-802.5F6102403GPC KF-2003≥ 18,000620.0 × 300KF-803F6102404GPC KF-2004≥ 14,000720.0 × 300KF-804F6102405GPC KF-2005≥ 10,0001020.0 × 300KF-805F6102406GPC KF-2006≥ 10,0001020.0 × 300KF-806F6102409GPC KF-2006M≥ 10,0001020.0 × 300KF-806MF6700406GPC KF-LG(guard column)158.0 × 50(guard column)82* See page 36 for GPC KF-800 series.*Contact Shodex or our distributors near you for customized columns.Base Material : Styrene divinylbenzene copolymer

Organic SEC (GPC) ColumnsPreparative ColumnsPreparativeColumnsPreparativeColumnsGPC K-800 series preparative typeProduct CodeF6102301F6102312F6102315F6102303F6102304F6102305F6102306F6102309F6700407Preparative Columns [Custom-made]Product CodeF6102001F6102002F6102025F6102003F6102004F6102005F6102006F6102009F6700310Product NameGPC K-2001GPC K-2002GPC K-2002.5GPC K-2003GPC K-2004GPC K-2005GPC K-2006GPC K-2006MGPC K-LGProduct NameGPC H-2001GPC H-2002GPC H-2002.5GPC H-2003GPC H-2004GPC H-2005GPC H-2006GPC H-2006MGPC H-GPlate Number(TP/column)≥ 18,000≥ 18,000≥ 18,000≥ 18,000≥ 14,000≥ 10,000Organic SEC (GPC) ColumnsPlate Number(TP/column)≥ 13,000≥ 13,000≥ 13,000≥ 13,000≥ 13,000≥ 13,000≥ 13,000≥ 12,000(guard column)Particle Size(µm)6666710101015Particle Size(µm)151515151515151515Column Size (mm)I.D. x Length20.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 30020.0 × 3008.0 × 50Column Size (mm)I.D. x Length20.0 × 50020.0 × 50020.0 × 50020.0 × 50020.0 × 50020.0 × 50020.0 × 50020.0 × 5008.0 × 50Standard ColumnK-801K-802K-802.5K-803K-804K-805K-806K-806M(guard column)* See page 38 for GPC K-800 series. Base Material : Styrene divinylbenzene copolymerGPC K-800 series preparative type≥ 10,000≥ 10,000(guard column)Standard ColumnK-801K-802K-802.5K-803K-804K-805K-806K-806M(guard column)PreparativeColumnsOrganic SEC (GPC) Columns Preparative Columns [Custom-made]* See page 38 for GPC K-800 series.GPC KF-800 series preparative typeProduct CodeF6108010F6108020F6108025F6108030F6108040F6700408Product NameGPC KF-5001GPC KF-5002GPC KF-5002.5GPC KF-5003GPC KF-5004GPC KF-LLGParticle Size(µm)151515151515Column Size (mm)I.D. x Length50.0 × 30050.0 × 30050.0 × 30050.0 × 30050.0 × 30020.0 × 100Base Material : Styrene divinylbenzene copolymerStandard ColumnKF-801KF-802KF-802.5KF-803KF-804(guard column)* See page 36 for GPC KF-800 series.Base Material : Styrene divinylbenzene copolymerGPC K-800 series preparative typeProduct CodeF6109010Product NameGPC K-5001Particle Size(µm)15Column Size (mm)I.D. x Length50.0 × 300Standard ColumnK-801F6109020GPC K-50021550.0 × 300K-802F6109025GPC K-5002.51550.0 × 300K-802.5F6109030GPC K-50031550.0 × 300K-803F6109040GPC K-50041550.0 × 300K-804F6700409 GPC K-LLG* See page 38 for GPC K-800 series.1520.0 × 100(guard column)Base Material : Styrene divinylbenzene copolymer83*Contact Shodex or our distributors near you for customized columns.

InformationPhase-out ProductsPhase-out products remain available to customers in need of them for the purpose of quality control, etc.For more information please contact Shodex or our distributors near you.Please note that phase-out products may go out of production in the future.Polymer-based Packed Columns forReversed Phase ChromatographyColumns for Rare Earth Metal IonsPhase-out ProductsProduct NameRSpak RP18-613RSpak RP18-413RSpak DE-413SRSpak GOLF-413Column Size(mm)I.D. x Length6.0 × 1504.6 × 1504.6 × 504.6 × 150Product NameIC R-621IC R-GColumn Size(mm)I.D. x Length6.0 × 504.6 × 10Aqueous SEC Semi-micro ColumnsColumn for OxyhalidesProduct NameSB401-4EColumn Size(mm)I.D. x Length4.6 × 250Product NameIC SI-91 4CColumn Size(mm)I.D. x Length4.0 × 100SB402.5-4ESB403-4ESB404-4ESB400G-4A4.6 × 2504.6 × 2504.6 × 2504.6 × 10Columns for Anions in WineProduct NameColumn Size(mm)I.D. x LengthMultimode ColumnsProduct NameMSpak GS-320 4BColumn Size(mm)I.D. x Length4.6 × 50WINE VH-anion 4D4.6 × 150MSpak GS-320 4D4.6 × 150WINE VH-anionG 4A4.6 × 10MSpak GS-320 2D2.0 × 15084

InformationColumns for Affinity ChromatographyColumns for Chiral SeparationProduct NameAFpak AAB-894AFpak AAF-894AFpak AAM-894AFpak AAP-894Column Size(mm)I.D. x Length8.0 × 508.0 × 508.0 × 508.0 × 50Product NameORpak CDA-453 HQORpak CDB-453 HQORpak CDC-453 HQORpak CD-GColumn Size(mm)I.D. x Length4.6 × 1504.6 × 1504.6 × 1504.6 × 10Phase-out ProductsAFpak AAV-8948.0 × 50AFpak ABA-8948.0 × 50AFpak ABT-8948.0 × 50AFpak ACA-894AFpak ACB-8948.0 × 508.0 × 50Column for Column Switching MethodAFpak AED-894AFpak AGA-894AFpak AGE-894AFpak AGT-8948.0 × 508.0 × 508.0 × 508.0 × 50Product NamePROTEIN KW-604SColumn Size(mm)I.D. x Length6.0 × 50AFpak AIA-8948.0 × 50AFpak ALC-8948.0 × 50AFpak ALS-8948.0 × 50AFpak ANA-8948.0 × 50AFpak AOV-8948.0 × 50AFpak APB-8948.0 × 50AFpak APD-8948.0 × 50AFpak APE-8948.0 × 50AFpak APH-8948.0 × 50AFpak APR-8948.0 × 50AFpak AST-8948.0 × 50AFpak AGO-4944.6 × 1085

InformationColumn Cleaning ProceduresChanges in peak shape, elution time, or elevated column pressure may be resolved by cleaning the column. Thissection describes general indications of column deterioration and column cleaning procedures.For details of column cleaning procedures, refer to each column’s specific operation manual.Typical indicators of column deterioration possibilityColumn Cleaning Procedures1. Elevated column pressure2. Abnormal peak shapes (broadening, leading, or tailing) and split peaks3. Change in retention time4. Unstable baselineSelection guide to the cleaning solventSolvents capable of dissolving the adsorbed substancesSolvents with high eluting power (variable depending on separation mode)*Use the solvent specified in the operation manual.Standard cleaning proceduresFor an efficient cleaning, reverse the direction and reduce the flow rate to 1/3 of the regular flow.Columns forreversed phasechromatographyColumns forsugar analysischromatographyColumns foraqueous SEC (GFC)chromatographyColumns forion exchangechromatographyClean the columns with solvent containing higher concentration of organic solvent such asmethanol, acetonitrile, or THF.(In case of using buffer as a mobile phase, miscibility of the buffer solution and the organicsolvents need to be checked)[Ligand exchange columns (SUGAR series)]• In case of counter ion detachmentFlush or inject solvent containing the salt corresponding to the modified counter-ligand.[Polymer-base amino columns (NH2P series)]• In cases where an acidic substance has been bound to the amino functional groupFlush with solvents in the following sequence: water, 0.1M perchloric acid (aq.), water,0.1M NaOH (aq.), water, and mobile phase.• In cases where an ionic substance has been adsorbedUse a solvent with higher salt concentration or solvent with different pH from the mobile phase.• In cases where a hydrophobic substance has been adsorbedUse a solvent containing organic solvent.(In case of using buffer as a mobile phase, miscibility of the buffer solution and the organicsolvents need to be checked)• In cases where an ionic substance has been adsorbedUse a solvent with higher salt concentration or solvent with different pH from the mobile phase.• In cases where a hydrophobic substance has been adsorbedUse a solvent containing organic solvent.(In case of using buffer as a mobile phase, miscibility of the buffer solution and the organicsolvents need to be checked)• In cases where protein have been adsorbedInject 1-2 mL of 0.1 M NaOH (aq.) or 30% (v/v) acetic acid (aq.) several times.Columns forhydrophobic interactionchromatography• In cases where protein have been adsorbedInject 1-2 mL of 0.1 M NaOH (aq.) or 30% (v/v) acetic acid (aq.) several times.*The required volume of the cleaning solvent is 5-10 times the column volume.*Avoid pressure elevation during the cleaning.*The cleaning is limited and does not guarantee the full regeneration of the column to its original condition.For your informationOne typical cause of the column pressure elevation is the clogging of solid substances at the inlet filter of the column.In this case, reverse the direction and reduce the flow to 1/3 of the regular flow rate. This may remove the solidsubstance causing the elevated pressure.86*Use the solvent specified in the operation manual.

General Precautions for Column HandlingFor the best performance of the column, please follow the instructions given below.InformationColumn mounting• Before mounting the column, replace the eluent within all the HPLC system with the mobile phase used for theanalysis. *If the mobile phase of the choice is not miscible with the eluent already in the system, use solvent that ismiscible with both solvents first to clean the system. *Buffer or salt solution may precipitate when mixed withorganic solvent of different concentrations.• Attach the column in the direction as indicated by arrow marked on the column. Gradually increase the flow rate ofthe solvent introduced to the column.• When heating the column, be sure to pump the eluent at a low flow rate until the specified temperature is reached,and then gradually increase the flow rate up to the requirement after the column has been heated sufficiently.Column dismounting• If the column is heated, turn off the heater while keeping the flow rate at 1/3 of the regular flow.• Turn off the pump when the column is cooled to room temperature.• Remove the column from the system and securely tighten the end caps.General Precautions for Column HandlingColumn storage• For long-term storage, replace the solvent with shipping solvent and securely tighten the end caps.• Store the column in a location with stable temperature.• For long-term storage of SEC columns, immersion method is recommended.*Please refer to the immersion method on the operation manual.Other• Avoid physical shock on the column. Be cautious not to drop the column from a high position.• Do not bend the column.• Avoid opening the column’s end-fitting, it can cause alteration of column’s performance.* Read the operation manual before using the column.87

InformationColumn Trouble ShootingCommon causes for abnormal chromatogramsUnstable baseline values······Main cause······Solution······Frequent problemColumn Trouble ShootingPeak tailingPeak split,shouldersPeakleadingBroad peakSelection ofcolumn and/or eluentappropriateProblem solvedby reducinginjection volumeNOYESNOYESStrong sample retentionVoid existing at top of columnAdsorption of impurities incolumnChanneling in columnLeading caused by sampleproperties(ionic and micellar properties)· Adsorption of impurities incolumn· Deterioration of columnSample overloadChange column and/or eluentReplace column· Wash column· Check column performance· Replace columnReplace columnChange column and/or eluent· Wash column· Check column performance· Replace columnReduce injection volumeGhost peakContaminationImpurities existing in eluentPrepare sample againWash flow line of HPLCReplace eluentNo peakSystem trouble· Eluent not flowing· Injector at wrong position (load/inject)· Detector deactivatedCheck systemSample adsorption in columnRepeat sample injection· Column equilibration insufficientPressure elevationPumpA B CColumn Detector······Main causeSTARTInjector······Solution······Frequent problemSalt precipitated isobserved in eluentYESPressure still highafter washingNOSalting out· Change eluent composition· Wash system before analysisNOYESPressure still high whenC is disconnectedNODetector pipingblocked· Clean up detector· Replace pipingYESPressure still high whenB is disconnectedYESNOColumnblockedPressure undermaximum columnpressureYESNOScreen eluent and samplethrough 0.45 μm filterYESNODeteriorationof columnColumn end-fittingblocked· Check column performance· Replace columnReplace columnPressure still high whenA is disconnectedNOInjector blocked· Clean up injector· Replace injectorYESUsed line filterYESNOPump piping blocked· Replace filter in pump· Clean up pump· Replace pump piping88Line filter blocked· Wash line filter· Replace line filter

HPLC System Trouble ShootingCommon causes for abnormal chromatogramsInformationUnstable baseline valuesSTART······Main cause······Solution······Frequent problemColumn PressurenormalYESBaseline stable whenpump stoppedYESNONOYESMonotonic driftNOMalfunction of check valveTemperature fluctuationDeterioration of detector lampInsufficient groundingMalfunction of recorderBubbles in flow cell· Replace check valve· Clean up check valve· Warm up detector sufficiently· Shield detector from wind· Check column oven· Check detector temperature controlReplace lampEstablish sufficient groundingRepair recorderWash inside of detectorHPLC System Trouble ShootingContamination of flow cellWash inside of detectorLeakage observedNOInsufficient eluent replacementWash flow line of HPLCYES(with different solvent, salt or others)Contamination of column· Wash column· Check column performance· Replace columnFlow rate fluctuationPulsation of pumpUse damperTighten connectionsDeterioration of eluent· Use eluent containing stabilizer(for THF, use THF containing BHT)Noise······Main cause······Solution······Frequent problemSTARTInsufficient groundingEstablish sufficient groundingBaseline stable whenpump stoppedNOIrregular noiseDeterioration of detector lampReplace detector lampYESMalfunction of recorderRepair recorderShodex DEGAS usedNOInsufficient degassing eluentUse Shodex DEGASYESSpike noiseYESOKSpike noise foundfrom beginingNOYESAir in columnProblem solved by applying backpressure to column(*)(*) Do not apply back pressure to detectorColumn temperature exceeding eluent boiling pointNO· Check column performance· Replace columnReduce temperature of columnoven and/or post-column reactioncoilNoise shape leadingNOContamination of flow cellWash flow cellYESBubbles in flow cellRepair detector89

IndexIndex by Product NameColumns are listed in alphabetical order under the product name excluding series name.[Series name]AFpakAsahipakAXpakCLNpakCXpakEPGPCHICICIECMSpakODSpakOHpakORpakPIKESSPROTEINRSpakSilicapakSTANDARDSTDSUGARUSPpakWINESilicaIndex by Product NameAAAB-894AAF-894AAM-894AAP-894AAV-894ABA-894ABT-894ACA-894ACB-894ACH-494ADS-894AED-894AGA-894AGE-894AGO-494AGT-894AHR-894AIA-894ALC-894ALS-894ANA-894AOV-894APA-894APB-894APD-894APG-894APH-894APR-894AST-894AT-806MS, AT-GAWG-894CC18MC18PC4P-50, C4P-50G 4A5C45C8C8P-50, C8P-50G 4ACDA-453 HQCDB-453 HQCDBS-453CDC-453 HQCD-GCM-8255CNCRX-853, CRX-G8585858585858585855656858585858556858585858556858556858585465614148141488585568585541456DDC-613, DC-GDE, DE-G, DE-SGDE-413SDEAE-2BDEAE3N-4TDEAE-825DM-614, DM-GDS-413, DS-613, DS-GEE-411ET-RP1ES-502C 7CES-502N 7CEV, EV-GFF-411, F-511, F-411/SFL-1, FL-1 filterFL-2, FL-2 filterGGF-1G 7BGF-210 HQGF-310 HQGF-310GF-4AGF-510 HQGF-710 HQGF-7M HQGOLF-413GS-220 HQGS-2G 7BGS-320 HQGS-320GS-520 HQGS-620 HQGS-620 7G-PHHFIP-603~607, HFIP-606M, HFIP-GHFIP-803~807, HFIP-806M, HFIP-LGHLDHT-803~807, HT-806M, HT-G1610, 5884525252101014565452621422, 2422, 2434343434, 586434343484323232843232324848644690

IndexII-524A, IA-GQ22 QA-82552JRJJ-50KK-801~807, K-803L~807L, K-806M, K-GK-800DKC-811, KC-G, KC-LGKD-801~807, KD-806M, KD-GKF-401HQ~404HQ, KF-405LHQ, 406LHQKF-601~607, KF-606MKF-800DKF-801~807, KF-803L~807L, KF-806MKF-GKS-801~806, KS-GKS-807, KS-807GKW402.5-4F~405-4F, KW400G-4AKW-604SKW-802.5~804, KW-GLLF-404, LF-604, LF-804, LF-GMM-75MN-43110, 583838, 40204042, 584236, 403636, 42, 581616288528445116R-621, R-GRP18-413, RP18-613RP18-415, RP18-GSSB401-4E~404-4E, SB400G-4ASB-802 H~Q806 HQ, SB-806M HQ, SB-GSB-807 HQ, SB-807GSC1011, SC1011-7F, SC1821, SC-LGSC1211, SC-GSH1011, SH1821, SH-GSH-75SI-35 4DSI-50 4ESI-52 4ESI-90 4ESI-91 4CSI-50G, SI-90G, SI-92G, SI-95G5SILSL-105SM-105SP0810, SP-GSP-2BSP-420NSP-825SZ5532, SZ-G848410843030161620512222222284221451511654545416Index by Product NameN5NHNH2P-40NH2P-50, NH2P-50GNH2P-LFNI-424, NI-GNN, NN-G5NPE 4D1412, 5812, 58122210, 5814TT-521, T-GUUT-802.5, UT-806M, UT-807, UT-GV2446OVH-anion 4D, VH-anionG 4A84ODP2 HP, ODP2 HPGODP-40ODP-50, ODP-50G6, 5888, 58WWA-624, WA-G52PYP-421S, P-GP-82PPAE, PAE-GPH-814PK5PYE 4D54515162566414Y-521, Y-GYK-421, YK-GYS-50, YS-G242424* Semi-micro and micro columns and preparative columns are not included.91

Technical InformationTechnical Notebook, Chromato NewsTechnical Notebook, Chromato NewsNotebookNo.12312346Technical NotebookChromato NewsHigh performance reversed phase column; polymer-based column Asahipak ODP-40Direct analysis of medical compounds in serum without sample pretreatmentSemi-micro GPC columns with high resolutionLinear calibration curves by GPC LF-804Food additivesTitleShodex GPC LF series columnsLinear calibration curves over a wide molecular weight rangeShodex NH2P-50 series columnsAnalysis of saccharides in food industryFood Analysis with Shodex columnsSaccharides, organic acids, vitamins, fatty acids and amino acids4 Shodex technical notebook 4 is no longer available 8NewsNo. Title ColumnNo.567ODP-40 seriesGF-310 series, GS-320 seriesKF-400HQ seriesLF-804TitleShodex KW400 series columnsHigh performance and downsized column for protein analysisShodex ODP2 HP series columnsBetter retention of highly polar substancesProtein & peptide with Shodex columnsShodex Ion Chromatography columnsAnion & cation analysis with suppressor & non-suppressorSP0810, SC1011, NH2P-50 4E, KC-8117Polymer-based column with multimode of reversed phase and ionic interactionNN series, JJ-508Ultra high molecular weight and water-soluble polymer with SUGAR KS-807KS-807, KS-800 series9Direct analysis of proteins and peptides in surfactants using surfactant removalcolumn MSpak PK seriesPK series, GF-4A10Direct analysis of additives in polymer with SEC/MSKF-400HQ series11Concentration of the hydrophilic medical compounds existing with proteinsPK series12Column selection for proteins and peptides analysisSEC, Reversed phase, Ion exchange, Affinity columns13Analysis of hyaluronic acid with ultra high molecular weight in serumKS-807, GS-620HQ14Water-soluble vitaminsNH2P-50 4E, DE-41317Saccharides and sugar alcohols with Asahipak NH2P-50 columnNH2P-50 series22Online removal of surfactants in protein analysisPK-2A, KW-604S23Various application with RSpak DE series; high pH, 0% organic eluentDE series24Analysis of amines and amides with RSpak DE seriesDE-613, DE-413, ACH-49425Simultaneous analysis of monosaccharide and organic acid with SUGAR SH1011SH101129Water-soluble ultra high molecular compounds with OHpak SB-807HQSB-807HQ, SB-806HQ30New reversed phase column ODP2 HPODP2 HP series31New protein analysis column KW400KW400 series32High sensitive analysis of pyridylaminated saccharidesNH2P-5033New rapid cation exchange column; DEAE3N-4T with non-porous gelDEAE3N-4T35Shellfish poisoning and pesticidesDE413-2B, ODP-50 4E36Suitable column for LC/MS application; ODP2 HPODP2 HP40Analysis of sugars in bioethanol productionSH1821, KS-802, LF-80441High temperature reversed phase column in high temperature for rapid analysisET-RP1 4D42Ion exchange columns for UHPLCPIKESS series43Saccharides analysis with H 2 O eluentSP0810, SC1011, KS-801, SH10119244High resolution separation by NH2P-40 3ENH2P-40 3E

Technical SupportTel: +49 (0) 89 9399 6237E-mail: info@shodex.deOrderwww.shodex.deTel: +49 (0) 89 9399 6234E-mail: order@shodex.deShowa Denko Europe GmbHKonrad-Zuse-Platz 481829 MunichGermany

Please contact a Shodex support officeSupport officeEuropeAfricaMiddle EastRussiaShowa Denko Europe GmbHKonrad-Zuse-Platz 4, D-81829 Munich, GermanyTEL : +49 (0)89 93 99 62-37 FAX : +49 (0)89 93 99 62-50E-mail : info@shodex.dewww.shodex.deNorth AmericaLatin AmericaShowa Denko America, Inc.420 Lexington Avenue, Suite 2335, New York, NY 10170, USATEL : +1 212 370 0033 FAX : +1 212 370 4566E-mail : support@shodex.netwww.shodex.netSoutheast AsiaIndiaTaiwanOceaniaShowa Denko K.K.23F Muza Kawasaki Central Tower,1310, Omiya-cho, Saiwai-ku, Kawasaki, Kanagawa 212-0014, JapanTEL : +81 (0)44 520 1380 FAX : +81 (0)44 520 1383E-mail : Sdk_Shodex@sdk.co.jpwww.shodex.comChinaHong KongMacauShodex China Co., Ltd.18F, Wang Wang Building, No.211 Shi Men Yi Road, Jing An,Shanghai, 200041, P.R.ChinaTEL : +86 (0)21 6217-6111 FAX : +86 (0)21 6217-9879E-mail : sales@shodexchina.comwww.shodex.com/index_ch.htmlKoreaShoko Korea Co., Ltd.#322, Chungjeong Rizion, 465, Chungjeongno 3-ga,Seodaemun-gu, Seoul, 120-013, KoreaTEL : +82 (0)2 784 5111 FAX : +82 (0)2 784 5125E-mail : shoko.korea@shokokorea.comwww.shodex.com/index_kr.htmlwww.shodex.deManufactured bySHOWA DENKO K.K.Shodex (Separation & HPLC) Group23F Muza Kawasaki Central Tower,1310, Omiya-cho, Saiwai-ku, Kawasaki, Kanagawa 212-0014, JapanTEL : +81-(0)44-520-1380 FAX : +81-(0)44-520-1383CAT.NO.01E. 15.JAN.13

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