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  • Forld Z7e2 - ' Rey - s/67

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    . Forld z7e2 -' Rey . s/67

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    16 views2 pages

    Forld Z7e2 - ' Rey - s/67

    Uploaded by

    isomerase

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 2

    .

    FORld Z7e2 -' REY . S/67


    m
    . ~$ .r--~•_-

    II~TER-®FFICE MEMORANDU M

    Subject : Glucose Isomerase : Evaluation'of Synthetic Date : March 23, 1970 •


    Anion Exchange Resins to Act as a Soli d
    Support Medium for the Enzym e

    To: Dr . Charles G . Pheil

    r
    Preliminary screening of several commercially available,'inexpen-
    L,ive anion exchange resins has been carried out . The stronCly basic
    macroreticular resins IRA-900, IRA-~910, and IRA-911 produced by Rohm and
    Haas-Company as well as the intermediate base Duolite A-57 resin produced
    by Diamond Shamrock Chemical Company have proved to be of no value for
    enzyme binding .
    .. .... . .
    The weakly basic, macroreticular anion exchange resin IRA-9 3
    by Rohm and Haas has been shown to possess considerable binding capacity
    for glucose isomerase . The most probable reason for the .more effective
    binding of IRA-93 over IRA-900 is the fact that the average pore diameter
    of IRA-93 is twice as large as that of IRA-900, allowing the penetration
    of the relatively large glucose isomerase molecule into the resin beads .

    A column cont ining 200 grams (wet weight) of IRA-93 to which


    has been adsorbed 6x10~ microunits of glucose isomerase has been operated
    for two weeks at 55°C for various flow rates . At fast flow rates,a low
    percent (10-20%) conversion of*glucose to fructose occurs . At slow flow
    rates (2 milliliters per minute),conversions as high as 40% have been
    achieved .

    It must be pointed out that these observations represent only


    preliminary screening efforts and cannot be compared to the .vast knowledge
    which has been gathered on DEAE cellulose by investigators in this research
    department .

    In summary, the following points may be made in favor .of further


    investigation of IRA-93 .

    1 . It is readily available in commercial quantities at low


    cost .

    2 . It has high chemical,stability including high resistanc e


    to oxidation :

    3. Its "spherical bead" shape and size allow for columns


    which could yield high flow rates .
    Dr . Pheil
    Page 2
    March 23, 1970

    4 . It may be washed and recharged with enzyme without removing


    it from the column .

    WSS :msc

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