VE-cadherin Antibody (F-8): sc-9989

VE-cadherin Antibody (F-8) is an IgG₁ κ mouse monoclonal VE-cadherin antibody (also designated CDH5 antibody, CD144 antibody or Cadherin 5 antibody) that detects the VE-cadherin protein of mouse, rat and human origin by WB, IP, IF, FCM and ELISA. VE-cadherin Antibody (F-8) is available as both the non-conjugated anti-VE-cadherin antibody form, as well as multiple conjugated forms of anti-VE-cadherin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The cadherins are a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy-terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including β-catenin, to regulate cadherin function. VE-cadherin (for vascular endothelial cadherin, also designated cadherin-5) is localized at intercellular junctions of endothelial cells, where it is thought to play a role in the cohesion and organization of intercellular junctions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Genomic organization and chromosomal mapping of the mouse P-cadherin gene.  |  Hatta, M., et al. 1991. Nucleic Acids Res. 19: 4437-41. PMID: 1886768
2. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.  |  Takeichi, M. 1988. Development. 102: 639-55. PMID: 3048970
3. Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin.  |  Breviario, F., et al. 1995. Arterioscler Thromb Vasc Biol. 15: 1229-39. PMID: 7627717
4. Desmosomal cadherins: another growing multigene family of adhesion molecules.  |  Koch, PJ. and Franke, WW. 1994. Curr Opin Cell Biol. 6: 682-7. PMID: 7833048
5. Cadherins and catenins: interactions and functions in embryonic development.  |  Ranscht, B. 1994. Curr Opin Cell Biol. 6: 740-6. PMID: 7833053
6. Spatial and temporal relationships between cadherins and PECAM-1 in cell-cell junctions of human endothelial cells.  |  Ayalon, O., et al. 1994. J Cell Biol. 126: 247-58. PMID: 8027182
7. Dynamics of cadherin/catenin complex formation: novel protein interactions and pathways of complex assembly.  |  Hinck, L., et al. 1994. J Cell Biol. 125: 1327-40. PMID: 8207061
8. Morphogenetic roles of classic cadherins.  |  Takeichi, M. 1995. Curr Opin Cell Biol. 7: 619-27. PMID: 8573335

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VE-cadherin Antibody (F-8) has 383 citations in a variety of scientific publications.

Primary antibodies like Anti-VE-cadherin Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VE-cadherin Antibody (F-8) Specifications

Alternate Product Name

Cadherin 5

Product Clone Name

F-8

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

768-784

Epitope Target

VE-cadherin

Epitope Species

human

Product Reactivity/Detection

VE-cadherin

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

porcine

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, FCM, ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

383 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred VE-cadherin antibody is VE-cadherin Antibody (BV9): sc-52751.