Thrombin R Antibody (ATAP2): sc-13503

Thrombin R Antibody (ATAP2) is an IgG₁ κ mouse monoclonal Thrombin R antibody (also designated thrombin receptor antibody, F2R antibody, coagulation factor II antibody, or PAR1 antibody) that detects the Thrombin R protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Thrombin R Antibody (ATAP2) is available as both the non-conjugated anti-Thrombin R antibody form, as well as multiple conjugated forms of anti-Thrombin R antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Thrombin is a serine protease that is involved in platelet aggregation and blood coagulation. It is cleaved from its precursor, prothrombin, and converts fibrinogen to Fibrin in the final step of the clotting cascade. Thrombin mediates its regulatory effects by activating cell surface receptors. These receptors, including Thrombin R (also designated PAR-1, for protease-activated receptor-1), PAR-2 and PAR-3, are members of the G protein-coupled receptor family, and share a similar gene structure. Thrombin cleaves its receptor, releasing a 41 amino acid peptide that acts as a platelet agonist. Upon this activation by thrombin, the Thrombin Rs trigger an increase in cytosolic Ca(2+) concentration. Unactivated Thrombin R cycles between the cell surface and an intracellular pool, while activated Thrombin R internalizes rapidly and is degraded in the lysosomes. The human Thrombin R is also known to be regulated by Sp1 and Sp3 transcription factors.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Differential transcriptional regulation of the human thrombin receptor gene by the Sp family of transcription factors in human endothelial cells.  |  Wu, Y., et al. 1998. Biochem J. 330 (Pt 3): 1469-74. PMID: 9494121
2. The cleaved peptide of the thrombin receptor is a strong platelet agonist.  |  Furman, MI., et al. 1998. Proc Natl Acad Sci U S A. 95: 3082-7. PMID: 9501219
3. Thrombin.  |  Goldsack, NR., et al. 1998. Int J Biochem Cell Biol. 30: 641-6. PMID: 9695019
4. Gene and locus structure and chromosomal localization of the protease-activated receptor gene family.  |  Kahn, ML., et al. 1998. J Biol Chem. 273: 23290-6. PMID: 9722561
5. Separate signals for agonist-independent and agonist-triggered trafficking of protease-activated receptor 1.  |  Shapiro, MJ. and Coughlin, SR. 1998. J Biol Chem. 273: 29009-14. PMID: 9786906
6. Analysis of a Ca2+-activated K+ channel that mediates hyperpolarization via the thrombin receptor pathway.  |  Sullivan, R., et al. 1998. Am J Physiol. 275: C1342-8. PMID: 9814983

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Thrombin R Antibody (ATAP2) has 193 citations in a variety of scientific publications.

Primary antibodies like Anti-Thrombin R Antibody (ATAP2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Thrombin R Antibody (ATAP2) Specifications

Alternate Product Name

thrombin receptor

Product Clone Name

ATAP2

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

42-55

Epitope Target

Thrombin R

Epitope Species

human

Product Reactivity/Detection

Thrombin R

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), FCM

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

193 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin