SYP/Synaptophysin Antibody (D-4): sc-17750

SYP Antibody (D-4) is an IgG₁ κ mouse monoclonal SYP antibody (also designated SYP antibody, SYPH antibody, Major Synaptic Vesicle Protein P38 antibody, MRX96 antibody, or MRXSYP antibody) that detects the SYP protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. SYP Antibody (D-4) is available as both the non-conjugated anti-SYP antibody form, as well as multiple conjugated forms of anti-SYP antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis. In addition, the tail domain of brain Myosin V also forms a stable complex with synaptobrevin II and SYP, and this complex is disassembled upon the depolarization-induced entry of Ca2+ into intact nerve endings.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. The synaptophysin-synaptobrevin complex: a hallmark of synaptic vesicle maturation.  |  Becher, A., et al. 1999. J Neurosci. 19: 1922-31. PMID: 10066245
2. Lipids, lipid modification and lipid-protein interaction in membrane budding and fission--insights from the roles of endophilin A1 and synaptophysin in synaptic vesicle endocytosis.  |  Huttner, WB. and Schmidt, A. 2000. Curr Opin Neurobiol. 10: 543-51. PMID: 11084315
3. Endocytosis of the synaptic vesicle protein, synaptophysin, requires the COOH-terminal tail.  |  Linstedt, AD. and Kelly, RB. 1991. J Physiol (Paris). 85: 90-6. PMID: 1757893
4. Torpedo synaptophysin: evolution of a synaptic vesicle protein.  |  Cowan, D., et al. 1990. Brain Res. 509: 1-7. PMID: 2106365
5. Vesicle-associated membrane protein and synaptophysin are associated on the synaptic vesicle.  |  Calakos, N. and Scheller, RH. 1994. J Biol Chem. 269: 24534-7. PMID: 7929121
6. Synthesis of the mammalian synaptic vesicle protein synaptophysin in insect cells: a model for vesicle biogenesis.  |  Leimer, U., et al. 1996. Exp Cell Res. 224: 88-95. PMID: 8612696
7. Brain myosin V is a synaptic vesicle-associated motor protein: evidence for a Ca2+-dependent interaction with the synaptobrevin-synaptophysin complex.  |  Prekeris, R. and Terrian, DM. 1997. J Cell Biol. 137: 1589-601. PMID: 9199173

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SYP/Synaptophysin Antibody (D-4) has 159 citations in a variety of scientific publications.

Primary antibodies like Anti-SYP/Synaptophysin Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SYP/Synaptophysin Antibody (D-4) Specifications

Alternate Product Name

Synaptophysin

Product Clone Name

D-4

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

221-313

Epitope Target

SYP

Epitope Species

human

Product Reactivity/Detection

SYP

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

159 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin