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Syk Antibody (4D10) - Western Blotting - Image 372650

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Syk Antibody (4D10): sc-1240. Near-infrared western blot analysis of Syk expression in BJAB (A), A-431 (B), THP-1 (C), Ramos (D) and NAMALWA (E) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214. Detection reagent used: m-IgGκ BP-CFL 680: sc-516180.

Syk Antibody (4D10): sc-1240

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Datasheets
  • Syk Antibody (4D10) is a mouse monoclonal IgG2a κ Syk antibody, cited in 266 publications, provided at 200 µg/ml
  • raised against amino acids 313-339 of Syk of human origin
  • Syk Antibody (4D10) is recommended for detection of Syk of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
  • Anti-Syk Antibody (4D10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Syk (4D10): sc-1240.
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Syk Antibody (4D10) for WB and IHC(P) applications. These reagents are now offered in bundles with Syk Antibody (4D10) (see ordering information below).

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Syk Antibody (4D10) is an IgG2a κ mouse monoclonal Syk antibody (also designated spleen tyrosine kinase antibody or p72-Syk antibody) that detects the Syk protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Syk Antibody (4D10) is available as both the non-conjugated anti-Syk antibody form, as well as multiple conjugated forms of anti-Syk antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Syk (spleen tyrosine kinase) is a 635 amino acid protein that contains one protein kinase domain and two SH2 domains. One of several members of the protein kinase superfamily, Syk functions as a positive effector of B cell antigen receptor (CD79)-stimulated responses, coupling CD79 with the movement of one calcium ion through one of two phospho-regulated pathways. Specifically, calcium ions travel through either a phosphoinositide 3-kinase (PI 3-kinase)-dependent pathway when Syk is not phosphorylated, or through a phospholipase C (PLC) γ-dependent pathway when human Syk is phohsphorylated on Tyr 348 and Tyr 352. Via its ability to influence CD79 activity and to control the movement of calicum through the cell, Syk plays an important role in a variety of cellular responses, including differentiation, phagocytosis, proliferation and B cell development. Syk expression is upregulated in T cell lymphoma, suggesting a possible role for Syk in tumorigenesis. Two isoforms of Syk, designated short and long, exist due to alternative splicing events.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

References:

  1. Association of the 72-kDa protein-tyrosine kinase PTK72 with the B cell antigen receptor.  |  Hutchcroft, JE., et al. 1992. J Biol Chem. 267: 8613-9. PMID: 1569106
  2. Syk is downstream of intercellular adhesion molecule-1 and mediates human rhinovirus activation of p38 MAPK in airway epithelial cells.  |  Wang, X., et al. 2006. J Immunol. 177: 6859-70. PMID: 17082600
  3. Deregulated Syk inhibits differentiation and induces growth factor-independent proliferation of pre-B cells.  |  Wossning, T., et al. 2006. J Exp Med. 203: 2829-40. PMID: 17130299
  4. Tyrosine kinase Syk associates with toll-like receptor 4 and regulates signaling in human monocytic cells.  |  Chaudhary, A., et al. 2007. Immunol Cell Biol. 85: 249-56. PMID: 17228323
  5. CD303 (BDCA-2) signals in plasmacytoid dendritic cells via a BCR-like signalosome involving Syk, Slp65 and PLCgamma2.  |  Röck, J., et al. 2007. Eur J Immunol. 37: 3564-75. PMID: 18022864
  6. Activation of Syk by protein kinase C-delta regulates thrombin-induced intercellular adhesion molecule-1 expression in endothelial cells via tyrosine phosphorylation of RelA/p65.  |  Bijli, KM., et al. 2008. J Biol Chem. 283: 14674-84. PMID: 18362147
  7. Overexpression of Syk tyrosine kinase in peripheral T-cell lymphomas.  |  Feldman, AL., et al. 2008. Leukemia. 22: 1139-43. PMID: 18401419
  8. Hepatitis C virus NS5A protein interacts with and negatively regulates the non-receptor protein tyrosine kinase Syk.  |  Inubushi, S., et al. 2008. J Gen Virol. 89: 1231-1242. PMID: 18420802
  9. Syk protein-tyrosine kinase is regulated by tyrosine-phosphorylated Ig alpha/Ig beta immunoreceptor tyrosine activation motif binding and autophosphorylation.  |  Rowley, RB., et al. 1995. J Biol Chem. 270: 11590-4. PMID: 7538118

How to grade the positivity in breast carcinoma cells

Asked by: Ghazala
Thank you for your question. You would require appropriate positive and negative controls to compare the signals between different samples. Recommended positive controls are NAMALWA cell lysate: sc-2234, BJAB whole cell lysate: sc-2207 or Raji whole cell lysate: sc-364236. We also recommend using direct conjugates for detection so that signal strength directly corresponds to the amount of protein present.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Is it possible for the Syk Antibody (4D10): sc-1240 to react with monkey tissue samples?

Asked by: Trav11
To inquire about additional species reactivity not listed on the webpage, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function. We will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 5 out of 5 by Shile012221 from Syk Antibody (4D10) Syk Antibody (4D10) (sc-1024) worked well in our Western blotting experiments.
Date published: 2021-01-22
Rated 4 out of 5 by caraaa from Detects SYK in cell lines with low baseline levels I tested SYK in a cell line with low baseline levels (according to CCLE). This antibody was able to detect bands at a dilution of 1:1000.
Date published: 2020-10-08
Rated 4 out of 5 by Biochemsitry from Works good! Good a free sample to test. Planing to use in future! For WB perferct!
Date published: 2018-02-13
Rated 5 out of 5 by HappyFlower from Great antibody for western blot The batch of antibody I have worked well on HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 500 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2017-11-16
Rated 5 out of 5 by Virginie from The antibody worked in western blot This antibody worked very well in western blot on mouse osteoclasts extract. We diluted it at 1/200 but it could be diluted more because the signal was strong.
Date published: 2017-10-25
Rated 5 out of 5 by Hua XU from It works great. I tested in PC3 cells. It worked well at 1:200 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by limans from worked well. I got this antibody as free samples and it worked very well for WB. I used 1:500 dilution.
Date published: 2017-03-15
Rated 5 out of 5 by dchan from Excellent antibody for westerns Probed this antibody at 1/1000 dilution for one hour and used the Kappa-binding HRP as secondary: the result was flawless. Excellent antibody for westerns.
Date published: 2017-03-08
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Syk Antibody (4D10) is rated 4.7 out of 5 by 19.
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