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Stat6 Antibody (D-1): sc-374021
- Stat6 Antibody (D-1) is a mouse monoclonal IgG2b κ Stat6 antibody, cited in 40 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 799-823 at the C-terminus of Stat6 of mouse origin
- Stat6 Antibody (D-1) is recommended for detection of Stat6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Stat6 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-374021 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Stat6 (D-1): sc-374021.
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Stat6 Antibody (D-1) for WB and IHC(P) applications. These reagents are now offered in bundles with Stat6 Antibody (D-1) (see ordering information below).
Stat6 Antibody (D-1) is an IgG2b κ mouse monoclonal Stat6 antibody (also designated IL-4 Stat antibody) that detects the Stat6 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Stat6 Antibody (D-1) is available as both the non-conjugated anti-Stat6 antibody form, as well as multiple conjugated forms of anti-Stat6 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of proteins that have been designated Stats (signal transducers and activators of transcription. The first members of this family to be described include Stat1α p91, Stat1β p84 (a form of p91 that lacks 38 COOH-terminal amino acids) and Stat2 p113. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Stat3, which becomes activated in response to epidermal growth factor (EGF) and interleukin-6 (IL-6), but not interferon-γ (IFN-γ) or Stat4, is an additional member of this family. It has been suggested that the phosphorylated forms of both Stat3 and Stat4 form homodimers as well as heterodimers with the other members of the Stat family, and that differential activation of different Stat proteins in response to different ligands should help to explain specificity in nuclear signaling from the cell surface. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Other members of the Stat family include Stat5, which has been shown to be activated by prolactin and by IL-3, and Stat6 (also designated IL-4 Stat), which is involved in IL-4-activated signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Tyrosine-phosphorylated Stat1 and Stat2 plus a 48-kDa protein all contact DNA in forming interferon-stimulated-gene factor 3. | Qureshi, SA., et al. 1995. Proc Natl Acad Sci U S A. 92: 3829-33. PMID: 7537377
- Cooperative transcriptional activity of Jun and Stat3 beta, a short form of Stat3. | Schaefer, TS., et al. 1995. Proc Natl Acad Sci U S A. 92: 9097-101. PMID: 7568080
- Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. | Schindler, C. and Darnell, JE. 1995. Annu Rev Biochem. 64: 621-51. PMID: 7574495
- Interleukin-3, erythropoietin, and prolactin activate a STAT5-like factor in lymphoid cells. | Pallard, C., et al. 1995. J Biol Chem. 270: 15942-5. PMID: 7608147
- Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation. | Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
- An interleukin-4-induced transcription factor: IL-4 Stat. | Hou, J., et al. 1994. Science. 265: 1701-6. PMID: 8085155
- Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. | Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
- Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. | Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455