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HLA-G Antibody (4H84): sc-21799
- HLA-G Antibody (4H84) is a mouse monoclonal IgG1 κ HLA-G antibody, cited in 75 publications, provided at 200 µg/ml
- raised against amino acids 61-83 of HLA-G of human origin
- HLA-G Antibody (4H84) is recommended for detection of HLA-G isoforms of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-HLA-G Antibody (4H84) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA-G (4H84): sc-21799.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HLA-G Antibody (4H84) for WB and IHC(P) applications. These reagents are now offered in bundles with HLA-G Antibody (4H84) (see ordering information below).
HLA-G Antibody (4H84) is an IgG1 κ mouse monoclonal HLA-G antibody (also designated HLA major histocompatibility complex, class I, G antibody, human leukocyte antigen (HLA) G antibody, 45 kDa transmembrane class I human leukocyte antigen G antibody, chromosome 6p22 gene complex class 1G antibody, placenta trophoblast marker antibody, or fetal derived placental cell marker antibody) that detects the HLA-G protein of mouse, rat and human origin by WB, IP, IF and IHC(P). HLA-G Antibody (4H84) is available as both the non-conjugated anti-HLA-G antibody form, as well as multiple conjugated forms of anti-HLA-G antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Major histocompatibility complex (MHC), human leukocyte antigen (HLA) molecules are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an α or heavy chain, and a non-covalently associated protein, β-2-microglobulin. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. | Fournel, S., et al. 2000. Tissue Antigens. 55: 510-8. PMID: 10902607
- HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. | Fuzzi, B., et al. 2002. Eur J Immunol. 32: 311-5. PMID: 11807769
- Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases. | Pangault, C., et al. 2002. Hum Immunol. 63: 83-90. PMID: 11821155
- Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule. | Lozano, JM., et al. 2002. AIDS. 16: 347-51. PMID: 11834945
- Disulfide bond-mediated dimerization of HLA-G on the cell surface. | Boyson, JE., et al. 2002. Proc Natl Acad Sci U S A. 99: 16180-5. PMID: 12454284
- Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. | Menier, C., et al. 2003. Hum Immunol. 64: 315-26. PMID: 12590976