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HDAC7 Antibody (A-7) - Western Blotting - Image 359942

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HDAC7 Antibody (A-7): sc-74563. Western blot analysis of HDAC7 expression in NIH: OVCAR-3 (A), HuT 78 (B), Ramos (C), Raji (D) and NCI-H929 (E) whole cell lysates.

Histone Deacetylase 7 (HDAC7) Antibody (A-7): sc-74563

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Datasheets
  • Histone Deacetylase 7 (HDAC7) Antibody (A-7) is a mouse monoclonal IgG2a κ Histone Deacetylase 7 (HDAC7) antibody, cited in 23 publications, provided at 200 µg/ml
  • raised against amino acids 163-435 of HDAC7 of human origin
  • Histone Deacetylase 7 (HDAC7) Antibody (A-7) is recommended for detection of HDAC7 of human origin by WB, IP, IF and ELISA
  • Anti-Histone Deacetylase 7 (HDAC7) Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-74563 X, 200 µg/0.1 ml)
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Histone Deacetylase 7 (HDAC7) (A-7): sc-74563.
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Histone Deacetylase 7 (HDAC7) Antibody (A-7) for WB applications. These reagents are now offered in bundles with Histone Deacetylase 7 (HDAC7) Antibody (A-7) (see ordering information below).

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HDAC7 Antibody (A-7) is an IgG2a κ mouse monoclonal HDAC7 antibody (also designated Histone Deacetylase 7 antibody) that detects the HDAC7 protein of human origin by WB, IP, IF and ELISA. HDAC7 Antibody (A-7) is available as both the non-conjugated anti-HDAC7 antibody form, as well as multiple conjugated forms of anti-HDAC7 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC7 is a histone deacetylase that interacts with the adaptor mSin3A. The interaction of HDAC7 with mSin3A suggests the association of multiple repression complexes of transcription factors.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

References:

  1. Isolation of a novel histone deacetylase reveals that class I and class II deacetylases promote SMRT-mediated repression.  |  Kao, HY., et al. 2000. Genes Dev. 14: 55-66. PMID: 10640276
  2. Nucleosome structural changes due to acetylation.  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
  3. A positive role for histone acetylation in transcription factor access to nucleosomal DNA.  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
  4. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
  5. Nucleosomal DNA regulates the core-histone-binding subunit of the human Hat1 acetyltransferase.  |  Verreault, A., et al. 1998. Curr Biol. 8: 96-108. PMID: 9427644
  6. Transcriptional activators direct histone acetyltransferase complexes to nucleosomes.  |  Utley, RT., et al. 1998. Nature. 394: 498-502. PMID: 9697775

Can the TransCruz reagent sc-74563 X be used in applications other than ChIP?

Asked by: AbPolly
Thank you for your question. Yes, the X form of the antibody can be used for any listed application. The dilution just needs to be changed as it is ten times as concentrated as the standard antibody.
Answered by: Technical Support 3
Date published: 2017-02-09
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Rated 3 out of 5 by from also detected another smaller size bandUsing human blood monocyte derived macrophages, we could detect a faint band at right size. 25 ug total cell lysate per lane, 1:500 1Ab dilution in milk, 2nd Ab 1:4000 CST anti-mouse, 10 min exposure. Also there is a slightly stronger band at size <25kda.
Date published: 2021-07-02
Rated 5 out of 5 by from works wellit works well for western blotting in human podocytes
Date published: 2019-11-14
Rated 5 out of 5 by from Great HDAC7 detectionThis antibody works great in WB. It will provide strong specific signals and can even be used in your staining procedures. This product has been cited several times.
Date published: 2017-01-27
Rated 5 out of 5 by from Good WBDistinct band obtained at ~110 KDa band observed on 10 ug MDA-MB-231 whole cell lysates at 1:1000 dilution.
Date published: 2016-12-12
Rated 4 out of 5 by from Good antibodyGood antibody, worked well in K-562 nuclear extracts -SCBT QC
Date published: 2015-03-23
Rated 4 out of 5 by from Worked well in transfected 293T cellsWorked well in transfected 293T cells -SCBT QC
Date published: 2015-03-12
Rated 4 out of 5 by from Strong bands in WB in Hela and MCF7 nuclearStrong bands in WB in Hela and MCF7 nuclear extracts -SCBT QC
Date published: 2013-10-12
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Histone Deacetylase 7 (HDAC7) Antibody (A-7) is rated 4.3 out of 5 by 7.
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