CD63 Antibody (MX-49.129.5): sc-5275

CD63 Antibody (MX-49.129.5) is an IgG₁ κ mouse monoclonal CD63 antibody that detects the CD63 protein of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA. CD63 Antibody (MX-49.129.5) is available as both the non-conjugated anti-CD63 antibody form, as well as multiple conjugated forms of anti-CD63 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Role of adaptor complex AP-3 in targeting wild-type and mutated CD63 to lysosomes.  |  Rous, BA., et al. 2002. Mol Biol Cell. 13: 1071-82. PMID: 11907283
2. Potential role for CD63 in CCR5-mediated human immunodeficiency virus type 1 infection of macrophages.  |  von Lindern, JJ., et al. 2003. J Virol. 77: 3624-33. PMID: 12610138
3. The tetraspanin CD63 enhances the internalization of the H,K-ATPase beta-subunit.  |  Duffield, A., et al. 2003. Proc Natl Acad Sci U S A. 100: 15560-5. PMID: 14660791
4. Peptide YY and neuropeptide Y induce villin expression, reduce adhesion, and enhance migration in small intestinal cells through the regulation of CD63, matrix metalloproteinase-3, and Cdc42 activity.  |  Lee, M., et al. 2005. J Biol Chem. 280: 125-36. PMID: 15504731
5. CD63 as an activation-linked T cell costimulatory element.  |  Pfistershammer, K., et al. 2004. J Immunol. 173: 6000-8. PMID: 15528334
6. CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen.  |  Israels, SJ. and McMillan-Ward, EM. 2005. Thromb Haemost. 93: 311-8. PMID: 15711748
7. The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture.  |  Nishikata, H., et al. 1992. J Immunol. 149: 862-70. PMID: 1634775
8. Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63.  |  Horejsí, V. and Vlcek, C. 1991. FEBS Lett. 288: 1-4. PMID: 1879540
9. CD63/Pltgp40: a platelet activation antigen identical to the stage-specific, melanoma-associated antigen ME491.  |  Azorsa, DO., et al. 1991. Blood. 78: 280-4. PMID: 2070066

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD63 Antibody (MX-49.129.5) has 528 citations in a variety of scientific publications.

Primary antibodies like Anti-CD63 Antibody (MX-49.129.5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD63 Antibody (MX-49.129.5) Specifications

Alternate Product Name

CD63

Product Clone Name

MX-49.129.5

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

Epitope Target

CD63

Epitope Species

human

Product Reactivity/Detection

CD63

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

528 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine

The preferred CD63 antibody is CD63 Antibody (E-12): sc-365604.