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α-actinin Antibody (H-2) - Western Blotting - Image 384018

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α-actinin Antibody (H-2) HRP: sc-17829 HRP. Direct western blot analysis of α-actinin expression in A-673 (A), L8 (B), K-562 (C), HeLa (D), A-10 (E) and SJRH30 (F) whole cell lysates.

α-actinin Antibody (H-2): sc-17829

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Datasheets
  • α-actinin Antibody (H-2) is a mouse monoclonal IgG1 κ α-actinin antibody, cited in 183 publications, provided at 200 µg/ml
  • raised against amino acids 593-892 of α-actinin of human origin
  • alpha-actinin Antibody (H-2) is recommended for detection of α-actinin isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-alpha-actinin Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α-actinin (H-2): sc-17829.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for α-actinin Antibody (H-2) for WB and IHC(P) applications. These reagents are now offered in bundles with α-actinin Antibody (H-2) (see ordering information below).

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α-actinin Antibody (H-2) is an IgG1 κ mouse monoclonal alpha-actinin antibody (also designated ACTN1 antibody, Actinin 1 antibody, Alpha actinin 1 antibody or Cytoskeletal Alpha-actinin antibody) that detects the alpha-actinin protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. α-actinin Antibody (H-2) is available as both the non-conjugated anti-alpha-actinin antibody form, as well as multiple conjugated forms of anti-alpha-actinin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

References:

  1. An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment.  |  Reinhard, M., et al. 1999. J Biol Chem. 274: 13410-8. PMID: 10224105
  2. Fine mapping of the alpha-actinin binding site within cysteine-rich protein.  |  Harper, BD., et al. 2000. Biochem J. 350 Pt 1: 269-74. PMID: 10926853
  3. Interactions of a hemidesmosome component and actinin family members.  |  Gonzalez, AM., et al. 2001. J Cell Sci. 114: 4197-206. PMID: 11739652
  4. Structural dynamics of alpha-actinin-vinculin interactions.  |  Bois, PR., et al. 2005. Mol Cell Biol. 25: 6112-22. PMID: 15988023
  5. Alpha-actinin and vinculin in human neutrophils: reorganization during adhesion and relation to the actin network.  |  Yυrυker, B. and Niggli, V. 1992. J Cell Sci. 101 (Pt 2): 403-14. PMID: 1629252
  6. alpha-Actinin-dependent cytoskeletal anchorage is important for ICAM-5-mediated neuritic outgrowth.  |  Nyman-Huttunen, H., et al. 2006. J Cell Sci. 119: 3057-66. PMID: 16820411
  7. Expression of human alpha-actinin in human hepatocellular carcinoma.  |  Nishiyama, M., et al. 1990. Cancer Res. 50: 6291-4. PMID: 2169343
  8. Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene.  |  Youssoufian, H., et al. 1990. Am J Hum Genet. 47: 62-72. PMID: 2349951
  9. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.  |  Knudsen, KA., et al. 1995. J Cell Biol. 130: 67-77. PMID: 7790378

α-actinin 抗体 (H-2) Alexa Fluor® 647の日本語SDSを送付お願いいたします。 ない場合は、日本の法規制に該当するか教えていただけますようお願いいたします。

Asked by: ハラレイコ
質問頂き、誠にありがとうございます。資料の申請、又は質問がございましたら、日本のディーラーを連絡し、お尋ねください。宜しくお願い致します。 Thank you very much for your question. If you would like to apply for materials or have any questions, please contact your Japanese dealer and ask. Thank you for your cooperation.
Answered by: Jiajun Z
Date published: 2023-11-29

Does this antibody detect all 4 isoforms of alpha-actinin?

Asked by: MsMoonlight
Thank you for your inquiry. This antibody has a C-terminal epitope (within amino acids 593-892 ), so all isoforms present in the c-terminal would be possible to be picked up. Unfortunately the antibody has not been epitope mapped, so the exact epitope is not known.
Answered by: Tech Service
Date published: 2019-02-22

Can I freeze sc-17829 antibody for long time storage?

Asked by: Carlaat
Thank you for your question. No, freezing the antibody will negatively impact its viability. Our antibodies are contained in buffer with PBS, gelatin and sodium azide. When frozen, they precipitate out of the solution and may denature. The antibodies are best stored at 4 °C and if handled properly will retain their reactivity for several years.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

From the description of applications I guess this antibody should work for IP, but I can't find any review comment on the result of IP. Do you have any test result picture or publication using this antibody for IP? Thanks!

Asked by: Yali
Thank you for your question. This antibody is currently recommended and warrantied for IP, however we are still gathering data to show on our website. Check back for updates! There is also at least one product citation using this antibody successfully for IP/WB. Please refer to PMID: 16966331 to see their data with this antibody.
Answered by: Technical Support
Date published: 2022-06-15

Can α-actinin Antibody (H-2): sc-17829 be used for detection of NOS1 of pig origin?

Asked by: jenni
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 4 out of 5 by from Good for WBGood antibody, worked well diluted 1:1000 for western blot
Date published: 2022-02-04
Rated 4 out of 5 by from Great featuresI receveid the trial sample of this antibody 2 weeks ago and i tested it on Melanoma cell lines A375 and SK-Mel-5 in Western Blot at 1:1000. With odissey scanner i obtained well defined band around 95kDa.
Date published: 2021-12-14
Rated 5 out of 5 by from Good for both IF and WBThis antibody worked in IF and WB both in human melanoma cell line MDA-MB-435S. It showed differential expression among the cell lines and the expression was in line with the mass spectrometry results.
Date published: 2021-06-07
Rated 5 out of 5 by from Great resultsI got a sample of a-actinin antibody to use as a loading control, and it has been very successful. I started with the recommended dilution and have had to dilute it much more to avoid oversaturating our ECL detection reagent. We will purchase the full size soon.
Date published: 2020-02-04
Rated 4 out of 5 by from Good antibodyThe antibody is pretty good. Depending on the length of exposure an extra band (or bands) can be seen, which makes it less than perfect
Date published: 2019-12-14
Rated 5 out of 5 by from Detects chicken α-actinin for Western BlottingUsed a 1:1000 dilution, was able to detect alpha-actinin within chicken DF1 cells by Western Blotting with excellent signal.
Date published: 2019-04-02
Rated 5 out of 5 by from great signal for mouse muscle samplesUsed to detect a-actinin in mouse muscle samples, signal looks great.
Date published: 2019-01-23
Rated 5 out of 5 by from realiable & strong signalused for WB 1:10000 in 2% nonfat-dried milk in various cell lines strong signal after a few seconds
Date published: 2018-08-01
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α-actinin Antibody (H-2) is rated 4.9 out of 5 by 30.
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