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TULP4 Polyclonal antibody

TULP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55155-1-AP

Synonyms

KIAA1397, tubby like protein 4, Tubby related protein 4, Tubby superfamily protein, TUBL4, TULP4, TUSP

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using TULP4 Polyclonal antibody (55155-1-AP)

    WB analysis of human skeletal muscle using 55155-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55155-1-AP (TULP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using TULP4 Polyclonal antibody (55155-1-AP)

    WB analysis of human brain using 55155-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55155-1-AP (TULP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TULP4 Polyclonal antibody (55155-1-AP)

    IHC staining of human kidney using 55155-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55155-1-AP (TULP4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TULP4 Polyclonal antibody (55155-1-AP)

    IHC staining of human kidney using 55155-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55155-1-AP (TULP4 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55155-1-AP targets TULP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tubby like protein 4
Calculated Molecular Weight 169 kDa
Observed Molecular Weight 169 kDa
GenBank Accession NumberNM_020245
Gene Symbol TULP4
Gene ID (NCBI) 56995
RRIDAB_10859078
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TULP4, also named as KIAA1397, TUBL4 and TUSP, belongs to the TUB family. It may be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. This antibody is specific to TULP4.

Protocols

Product Specific Protocols
WB protocol for TULP4 antibody 55155-1-APDownload protocol
IHC protocol for TULP4 antibody 55155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols