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SRMS Polyclonal antibody

SRMS Polyclonal Antibody for IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26447-1-AP

Synonyms

C20orf148, SRM, SRMS, Tyrosine protein kinase Srms

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HL-60 cells using SRMS Polyclonal antibody (26447-1-AP)

    IP experiment of HL-60 using 26447-1-AP

    IP result of anti-SRMS (IP:26447-1-AP, 4ug; Detection:26447-1-AP 1:300) with HL-60 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRMS Polyclonal antibody (26447-1-AP)

    IHC staining of human breast cancer using 26447-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26447-1-AP (SRMS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRMS Polyclonal antibody (26447-1-AP)

    IHC staining of human breast cancer using 26447-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26447-1-AP (SRMS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IP detected inHL-60 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26447-1-AP is SRMS Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRMS fusion protein Ag24062
Full Name src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites
Calculated Molecular Weight 488 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC153198
Gene Symbol SRMS
Gene ID (NCBI) 6725
RRIDAB_2880516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SRMS antibody 26447-1-APDownload protocol
IP protocol for SRMS antibody 26447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols