Dr. V. Sathiesh Kumar’s Post

Here's to growth, collaboration, and advancing the field of microbiology.

Clinical Microbiology is a Rapidly Changing and Upgrading Field and One Must Stay Abreast with Developments in the Areas of their Interest. Glad to Share the Certificate of Completion for CLSI M100 S34 Changes in 2024. #Antimicrobials #Antibiotics #antimicrobialstewardship

"Applications of bioassay in microbiology." Bioassay in microbiology is a method of quantitatively determining the potency or effectiveness of a substance, such as a drug or a toxin, by measuring its biological effects on living cells or organisms. In microbiology, bioassays are often used to assess the ability of antimicrobial agents to inhibit or kill microorganisms. The bioassay typically involves exposing microorganisms to different concentrations of the substance being tested and observing the resulting effect on growth or survival. The results of the bioassay can then be used to calculate the minimum inhibitory concentration (MIC) or minimum bactericidal concentration (MBC) of the substance. Bioassays can be performed in vitro, using laboratory-grown cultures of microorganisms, or in vivo, using living animals or humans. In vitro assays are generally faster, cheaper, and less ethically problematic, but may not accurately reflect the in vivo effects of the substance. P.S: Overall, bioassays are an important tool in microbiology for assessing the potency and effectiveness of substances, and can be used to help guide the development of new antimicrobial agents or evaluate the effectiveness of existing therapies. #growth #development #help

happy to share the recent article has accepted in Journal of Pure and Applied Microbiology (JPAM) for publication. here is find the Abstract Tuberculosis is a contagious disease that is a significant cause of illness worldwide and has been declared one of the top ten causes of mortality across the world. It is well known that bacteria within biofilms exhibit much higher drug resistance than individual cells. Biofilms constitute a significant threat in the clinical environment by acting as reservoirs of multidrug-resistant bacteria. Thus, the formation of biofilms has been postulated to further aid in drug insensitivity and bacterial persistence within host tissues. The rapid increase in drug resistance in Mycobacteria poses a significant challenge to T.B. eradication and needs to addressed soon. In this review, we have attempted to frame a general overview of mycobacterial pathogenesis, the role of biofilm formation in enhancing its shelf life, and some natural compounds and nanoparticles as emerging novel therapeutics reported to inhibit biofilm formation in mycobacteria. Therefore, we present some recent advances which might have potential applications in new treatment regimens for Tuberculosis. Congratulations to all and stay tuned for more update.