Product/Service

2TdT In Situ Apoptosis Detection Kits

2TdT In Situ Apoptosis Detection Kits
The TACS 2 TdT Kits utilize Trevigen's unique cation optimization system to enhance label-ing within particular
The TACS 2 TdT Kits utilize Trevigen's unique cation optimization system to enhance label-ing within particular tissues. The TACS 2 TdT Kits employ a proprietary labeling buffer that contains no toxic components ( e.g.sodium cacodylate). A highly purified form of the TdT enzyme is included in the kits for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is achieved using streptavidin-horseradish peroxidase, and colorimetric sub-strates diaminobenzidine (DAB) or TACS Blue Label. For fluorescent detection, a fluores-cein conjugate of streptavidin is used and visualized by epifluorescence microscopy. Additional fluorophores are now available separately from Trevigen.

These complete kits provide all the reagents required for labeling including two permeabi-lization reagents, labeling and stop buffers, labeling and detection reagents, and TACS-Nuclease reagents for generating positive controls with your own samples. Please refer to our components listing for details.

The core kit is for researchers familiar with in situlabeling who are already set up for immunohistochemistry. A replenisher kit is also available for users of our TACS kits who have used up the limiting reagents within the kit. This kit provides a cost effective replace-ment of the reagents that are used most quickly.

Features:

  • TdT based in situlabeling.
  • Cation optimization for high signal and low background.
  • Complete kit provides either DAB, TACS Blue Label, or fluorescence detection options.
  • Includes exclusive Cytonin permeabilization reagent.
  • Includes TACS-Nuclease control reagents.

Applications:

  • In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
  • Light microscopy
  • Fluorescence microscopy
  • Flow cytometry

Items not included: Reagents for dehydration and rehydration, H2O2 , PBS buffer, and disposables.

Storage: Components are stored at -20°C, 4°C, and room temperature.

N/A, 8405 Helgerman Ct., Gaithersburg, MD 20877. Tel: 800-873-8443; Fax: 301-216-2801.