Accepted Manuscript
Title: Artemisia campestris L.: Ethnomedicinal,
phytochemical and pharmacological review
Author: Ikram Dib Luc Angenot Atika Mihamou Abderrahim
Ziyyat Monique Tits
PII:
DOI:
Reference:
S2210-8033(16)30083-5
http://dx.doi.org/doi:10.1016/j.hermed.2016.10.005
HERMED 158
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19-10-2016
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Please cite this article as: Dib, Ikram, Angenot, Luc, Mihamou, Atika,
Ziyyat, Abderrahim, Tits, Monique, Artemisia campestris L.: Ethnomedicinal,
phytochemical and pharmacological review.Journal of Herbal Medicine
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Artemisia campestris L.: Ethnomedicinal,
phytochemical and pharmacological review
Ikram Diba, Luc Angenotb, Atika Mihamouc, Abderrahim Ziyyata* and
Monique Titsb
a
Laboratoire
de
Physiologie
et
Ethnopharmacologie
URAC-40,
Département de Biologie, Faculté des Sciences, Université Mohammed
Premier, Oujda-Maroc
b
Phytochimie et Phytothérapie, Laboratoire de Pharmacognosie,
Département de Pharmacie, Université de Liège, Belgique
c
Laboratoire de Biologie des Plantes et des Microorganismes,
Département de Biologie, Faculté des Sciences, Université Mohammed
Premier, Oujda-Maroc
* Corresponding author :
Pr Abderrahim ZIYYAT
Laboratoire de Physiologie et Ethnopharmacologie, Département de Biologie, Faculté
des Sciences, Université Mohamed Premier, Oujda - Maroc
Phone: + (212) 6 67 08 61 22
Email 1: ziyyat@fso.ump.ma
Email 2 : ziyyat@yahoo.fr
1
Artemisia campestris L.: Ethnomedicinal,
phytochemical and pharmacological review
Ikram Diba, Luc Angenotb, Atika Mihamouc, Abderrahim Ziyyata* and
Monique Titsb
a
Laboratoire de Physiologie, Génétique et Ethnopharmacologie URAC-40,
Département de Biologie, Faculté des Sciences, Université Mohammed
Premier, Oujda-Maroc
b
Laboratoire de Pharmacognosie, Département de Pharmacie, Université
de Liège, Belgique
c
Laboratoire de Biologie des Plantes et des Microorganismes,
Département de Biologie, Faculté des Sciences, Université Mohammed
Premier, Oujda-Maroc
* Corresponding author :
Pr Abderrahim ZIYYAT
Laboratoire de Physiologie, Génétique et Ethnopharmacologie – URAC 40,
Département de Biologie, Faculté des Sciences, Université Mohamed Premier, Oujda Maroc
Phone: + (212) 6 67 08 61 22
Email : ziyyat@yahoo.fr
2
Abstract
Artemisia campestris L. (Asteraceae) is a perennial herb, commonly known as field
wormwood. It is widespread in Asia, North America, Europe and North Africa. The
different parts of this plant are used as anthelmintic, antidiabetic, antihypertensive,
emmenagogue, antivenom, and to treat digestive and cutaneous problems. An
exhaustive bibliographic research of this plant has been carried out by means of
scientific engines and databases like Google Scholar, PubMed, Science direct and
SciFinder; as a result, it has been found that this herb possesses a rich phytochemical
content and a wide range of pharmacological activities such as antioxidant, insecticidal,
antibacterial, antimutagenic, antivenom and antitumor effects. In an aim to highlight the
importance of A. campestris L., this review has been established by discussing its
ethnomedicinal, morphological, ecological, phytochemical, pharmacological and
toxicological studies.
Key words: Artemisia campestris L., Ethnomedicine, Ecology, Phytochemistry,
Pharmacology.
3
Table of contents
Abstract ......................................................................................................................................... 3
1.
Introduction ......................................................................................................................... 6
2.
Methodology ....................................................................................................................... 6
3.
Morphological description of Artemisia campestris L........................................................ 7
4.
Ethnomedicinal uses of Artemisia campestris L. ................................................................ 9
5.
Eco-geographical features of Artemisia campestris L. ..................................................... 10
Phytochemistry of Artemisia campestris L. ................................................................................ 11
6.
5.6.
Flavonoids ................................................................................................................... 11
5.6.
Phenolic acids.............................................................................................................. 13
5.6.
Coumarins and isocoumarins ...................................................................................... 14
5.6.
Other compounds ........................................................................................................ 14
5.6.
Volatile compounds..................................................................................................... 15
Pharmacology of Artemisia campestris L. ........................................................................ 20
6.1.
Antioxidant activity ..................................................................................................... 20
6.1.1.
DPPH (diphenyl picrylhydrazyl) radical scavenging activity ............................. 20
6.1.2. ABTS+ (2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) free radical
scavenging activity............................................................................................................ 21
6.1.3.
Other in-vitro antioxidant effects ........................................................................ 22
6.2.
Antibacterial activity ................................................................................................... 23
6.3.
Antifungal activity....................................................................................................... 25
6.4.
Insecticidal activity ..................................................................................................... 26
6.5.
Anthelmintic activity ................................................................................................... 27
6.6.
Antitumor activity ....................................................................................................... 27
6.7.
Antihypertensive effect ............................................................................................... 28
7.
Toxicology of Artemisia campestris L.............................................................................. 29
8.
Conclusion and perspectives ............................................................................................. 29
Conflict of interest....................................................................................................................... 30
4
Acknowledgements ..................................................................................................................... 30
References ................................................................................................................................... 31
5
1.
Introduction
Artemisia campestris L. is an Asteraceae species, with a large distribution in the
northern hemisphere; it displays a wide repartition in Eurasia and predominates the arid
regions of North Africa. Artemisia campestris has many medicinal actions, including:
antivenom, anticancer, antidiabetic, antihypertensive, anthelmintic, antimicrobial,
antifungal, and has been utilized in treating many other conditions, including: digestive,
respiratory, cutaneous and genital diseases. The phytochemical profile of A. campestris
L. has shown abundance in flavonoids, phenolic acids, coumarins, isocoumarins, fatty
acids, as well as a high content of monoterpenes and sesquiterpenes contained in the
essential oil. The pharmacological activities spans a wide range of potentials uses such
as antioxidant, antifungal, insecticidal, antibacterial, antimutagenic, antitumor,
anthelmintic and antihypertensive. The information above is fully referenced in the
body text.
This review gives a detailed insight about the species A. campestris L. that covers its
botany, folk-medicinal uses, ecology, phytochemistry, pharmacology and toxicology.
2.
Methodology
A comprehensive bibliographic research was conducted to give an in-depth insight into
the medicinal plant A. campestris L. and its related subspecies. Our literature research
has been carried out by means of the scientific engine Google Scholar
(http://scholar.google.com),
and
via
(http://www.ncbi.nlm.nih.gov/pubmed), Scopus
Direct
(http://www.sciencedirect.com),
the
databases,
PubMed
(http://www.scopus.com), Science
and
SciFinder
(http://www.libnet.ulg.ac.be/en/eresources/scifinder-scholar). This review investigates
6
the ethnomedicinal, morphological, ecological, phytochemical, pharmacological and
toxicological studies reported on A. campestris L. The references compiled in this
review contain 103 papers, including 94 original articles, 2 review articles, 3 books and
4 books references, dating from 1863 until July 2015. All full texts and informative
abstracts of studies dealing with A. campestris L. have been retained, among which 10
documents that have been written in French (6 articles and 4 books), while the
remainder of citations are in English. Unpublished thesis works and congresses
communications have been excluded. The chemical structures have been revised by
consulting
the
open
chemistry
database
PubChem
(http://pubchem.ncbi.nlm.nih.gov/search/#collection=compounds), and then redrawn
using the freeware version of the software ACD/ChemSketch (Freeware) 14.01.
(Chemical structures available in supplementary data on-line)
3.
Morphological description of Artemisia campestris L.
A. campestris L. is a perennial undershrub, that may reach 30-150cm in height, with
branched and ascending stems that form a panicle shape; it is usually brownish-red and
glabrous, and acquires a lignified form in the inferior part and a pubescent one at the top
(Chalchat et al. , 2003; De Lamarck and De Candolle, 1815; Quézel and Santa, 1962).
The leaves are green, sericeous when young, often glabrescent when mature; the basal
leaves are 2-3 pinnatisect, petiolate or even auricled, the upper are most simple.
(Chalchat et al., 2003; Quézel and Santa, 1962) (Fig.1A and 1B). The plant has a
composed inflorescence: the capitulum (Fig. 1C), ovoid and heterogameous, containing
8 to 12 flowers, organized on convex and glabrous receptacles, and surrounded by
involucral glabrous bracts organized in several rows. The ray flowers are female,
pistillate and fertile, while the disk flowers are sterile, and functionally male with
7
reduced abortive ovaries (Chalchat et al., 2003; Gillet and Magne, 1863; Ouyahya,
1990; Quézel and Santa, 1962). The male flowers are tubular, yellowish, lacking calyx,
with 5 fused petals, and 5 fused stamens, with the presence of secretory sacs on corolla
lobes of disk flowers (Minami et al. , 2010). The fruit is an ovoid achene lacking pappus
(Kreitschitz and Vallès, 2007).
According to Tutin et al. (1976) A. campestris L. is a polymorphic species and is
segregated into six subspecies that can be distinguished by morphological and
karyological data: Artemisia campestris (a) Subsp. campestris L., (b) Subsp. glutinosa
(Gay ex Besser) Batt., (c) Subsp. maritima Arcangeli; (d) Subsp. borealis (Pallas) Hall
& Clements; the main subspecies’ discussed in this paper are Subsp campestris,
glutinosa and maritima since they are the most studied subspecies in the literature.
(a) Subsp. campestris L.: is distinguished by a glabrescent stems and leaves, fleshy and
keeled beneath leaf-lobes which are thinly lanate when young, wide panicle, shortly
pedunculate capitula with an involucre width 1.5-2.5 mm, rarely hairy. The cytotype of
this subspecies is 2n=36 (D'Andrea et al. , 2003). The other subspecies have the same
characteristics as Subsp. campestris L. with some differences; for (b) Subsp. glutinosa
(Gay ex Besser) Batt., the panicle-branches and involucral bracts are viscid, besides, the
capitula are sessile or subsessile, but like (a), the cytotype is 2n=36 (Bellomaria et al. ,
2001). Concerning (c) Subsp. maritima Arcangeli, the morphological differences
consist of a fleshy but not keeled beneath leaf-lobes with a convex shape, and with a
velutinous aspect when young, also the capitula are often curved. For this subspecies the
main difference is the karyological number which is estimated as 2n=54 (Kreitschitz
and Vallès, 2003). The Subsp. borealis (Pallas) Hall & Clements (d), are without fleshy
8
leaf-lobes, with an involucre width of 5-6mm and the outer bracts almost entirely
herbaceous. The cytotype is 2n= 18 or 36.
4.
Ethnomedicinal uses of Artemisia campestris L.
A.campestris L. is commonly used as an anthelmintic and a treatment for cutaneous,
respiratory and digestive problems in many countries like Morocco, Algeria, Tunisia,
Libya, Spain, Turkey, Italy, Serbia, India, Argentina, Canada and United States (Ben
Sassi et al. , 2007; Boulanouar et al. , 2013; De Natale and Pollio, 2012; El Hassani et
al. , 2013; Fakchich and Elachouri, 2014; Gast, 1989; Hammiche and Maiza, 2006;
Kujawska and Hilgert, 2014; Leporatti and Ghedira, 2009; Popović et al. , 2012;
Shemluck, 1982; Tlili et al. , 2013). It is used as an antidiabetic and antihypertensive in
Morocco, Algeria and Japan (Aniya et al. , 2000; Ben Sassi et al., 2007; Bnouham et al.
, 2002; Boudjelal et al. , 2013; Djidel et al. , 2009), and is used to treat urinary, kidney
and liver disorders in Japan, Algeria and Tunisia (Aniya et al., 2000; Ben Sassi et al.,
2007; Benchelah et al. , 2004; Ferchichi et al. , 2006; Minami et al., 2010). It has an
extensive use as an emmenagogue and as a circulation regulator, especially in postpartum care, in Algeria (Benchelah et al., 2004; Boulanouar et al., 2013; Gast, 1989;
Hammiche and Maiza, 2006) and Serbia (Benítez et al. , 2010; Popović et al., 2012).
Furthermore, it is used as a febrifuge in Algeria (Boulanouar et al., 2013), Tunisia (Ben
Sassi et al., 2007) and Italy (Guarino et al. , 2008) and is utilized as an anti-venom in
Tunisia (Ben Sassi et al., 2007) and India (Bahekar et al. , 2012). It possesses other
traditional actions, such as anti-inflammatory and tonic (Ben Sassi et al., 2007; Popović
et al., 2012), as an analeptic (Hammiche and Maiza, 2006) and it is also recommended
in the treatment of eye diseases (El Hassani et al., 2013) (Table 1).
9
5.
Eco-geographical features of Artemisia campestris L.
Ecologically, A. campestris L. is able to thrive in an extremely large range of ecological
habitats, from thermo- Mediterranean scrub to mountainous belts and from Saharan to
humid zones (Subally and Quézel, 2002); it was also reported, that this species can be
found in the Supra-mediterranean bioclimatic storey upwards (Cariñanos et al. , 2013).
It prefers open habitats like meadow, forest glades and forest edges, and grows mainly
on dry soils (Nobis et al. , 2014). In fact, it was described that A. campestris L. occurs in
different forms depending on its habitat and ecotypes; as a result, four forms were
identified; (i) a shifting dune form related to the dune ecotype, (ii) a salty form that
grows in the salt meadows along the coast, (iii) a field form present in dry pastures and
gravelly field, and finally, (iv) a calcareous form that exists in the calcareous rock fields
and barren grounds (Turesson, 1925). Its occurrence is in fact conditioned by edaphic
rather than climatic criteria (Subally and Quézel, 2002).
Geographically, A. campestris L. predominates in the arid regions of North African
countries (Noumi et al. , 2010) like Morocco (Fakchich and Elachouri, 2014), Algeria
(Rebbas and Bounar, 2014), Tunisia (Kawada et al. , 2012; Saadaoui et al. , 2014), and
Libya (El-Mokasabi, 2014). It grows in dry, base-rich grasslands throughout much of
Central and Southern Europe (Pirini Chrisoula et al. , 2014); it is considered a ruderal
plant, in the disturbed dry river lands of Southern Spain (Salinas and Guirado, 2002). It
accompanies the dominant vegetation in xerophilous grasslands in the Czech Republic
(Novák and Konvička, 2006) and grows on gravelly soils near the rivers Tammaro and
Calore in Italy (Guarino et al., 2008), while, in Japan, it grows wild along the coastline
of the Ryukyu Islands (Minami et al., 2010). It represents the native forb species that
persists in reference sites of restored dunes in Great Lake in North America (Emery and
10
Rudgers, 2010). It is noteworthy to mention that A. campestris L. subspecies have
different geographical distribution. D'Andrea et al. (2003) reported that the Subsp.
campestris have a wide range of distribution in Europe, Northern and Central Asia and
North Africa, while, the Subsp. glutinosa seems to be limited to Europe, from Siberia to
Great Britain and in northern Africa (Juteau et al. , 2002; Masotti et al. , 2012). This
subspecies has also been described by Le Houerou (2013) as a dwarf shrub that belong
to steppes of the arid and desert zone of the near east and north Africa. The subspecies
maritima predominates the maritime sands of all the European Atlantic coast and
northwards to the Netherlands (Kreitschitz and Vallès, 2003; Tutin et al., 1976), and the
subspecies borealis exists mainly in the Alps and Arctic Russia (Tutin et al., 1976).
Phytochemistry of Artemisia campestris L.
5.6.
Flavonoids
A. campestris L. consists of many taxa, among which the subspecies glutinosa,
campestris and maritima, that have been profoundly studied for their flavonoid profile.
It has been reported that A. campestris L. contained a high amount of flavones, such as:
chrysin, apigenin, 6-methoxyapigenin (hispidulin),7,4’-O-dimethyl apigenin, 7-Omethyl 8-hydroxyapigenin, 7-O-methyl 6-methoxyapigenin (cirsimaritin), 4’-O-methyl
6-methoxyapigenin,
7-O-hexoside
apigenin,
6,8-di-C-glucoside
glucuronide apigenin, 6-C-glucuronide 8-C-pentoside apigenin,
apigenin,
7-O-
linderoflavone
B,
ficine, isoficine, luteolin, 6-methoxyluteolin (eupafolin), 7-O-methyl6-methoxyluteolin
(cirsiliol),
7,3’-O-dimethyl
6-methoxyluteolin
(cirsilineol),
7,4’-O-dimethyl
6-
methoxyluteolin (eupatorin), 7,3’,4’-O-trimethyl 6-methoxyluteolin, acetylglucuronide,
glucoside 6-methoxyluteolin (eupafolin glucoside) and 7-O-rutinoside luteolin. On the
other hand, the flavonols subclass seems to be represented mainly by kaempferol and its
11
derivatives: 7-O-methyl kaempferol, 3,7,-O-dimethyl kaempferol, 3,7,4’-O-trimethyl
kaempferol, 3,7,4’-O-trimethyl 6-methoxykaempferol, 3-rhamnoside kaempferol, 3rutinoside kaempferol,
(quercetagetin),
by quercetin and its
3’-O-methyl
quercetin
derivatives:
(isorhamnetin),
6-hydroxyquercetin
7-O-methyl
quercetin
(rhamnetin), 4’-O-methyl quercetin (tamarixetin), 3,3’-O-dimethyl quercetin, 3-Omethyl 6-methoxyquercetin (axillarin), 3-O-glucuronide quercetin, 3-rutinoside
quercetin (rutin), 3-galactoside quercetin (hyperoside), 3’-O-methyl hexoside quercetin,
and by myricetin and its derivatives: 3’-O-methyl myricetin (laricitrin), 4’-O-methyl
myrecitin (mearnsetin), and 3’,4’-O-dimethyl 6-methoxymyricetin (Akkari et al. , 2014;
Djeridane et al. , 2007; El-Ghazouly and Omar, 1984; Karabegović et al. , 2011;
Megdiche-Ksouri et al. , 2015; Sebai et al. , 2014). Only the dihydroflavone hesperidin
has been reported to exist in A.campestris L., while the 7-O-methyl taxifolin was the
only representative of the dihydroflavonols sub-class (Akkari et al., 2014).
For A. campestris subsp. glutinosa, in the acetone extract of the aerial part, the flavones
chrysin, apigenin, 7-O-methyl apigenin, 6-methoxyapigenin (6-O-methyl scutellarin or
hispidulin), luteolin, 6-hydroxyluteolin and 7,4’-O-dimethyl 6-methoxyluteolin have
been identified (De Pascual Teresa et al. , 1986; Valant-Vetschera et al. , 2003), instead,
6-methoxyapigenin (hispidulin) has been found in the chloroformic extract of the
flowering tops and the chloroform extract of the aerial part (De Pascual Teresa et al.,
1986; Hurabielle et al. , 1982). Other authors reported the existence of flavonols in the
hexane extract of this plant, like, 7-O-methyl kaempferol, quercetin, 3-O-methyl
quercetin, 7-O-methyl quercetin (rhamnetin), 7, 3’-O-dimethyl quercetin, (De Pascual
Teresa et al., 1986; 1984; González et al. , 1983; Valant-Vetschera et al., 2003). In the
acetone, hexanic and chloroformic extracts of the aerial part or the flowering tops, eight
12
dihydroflavones have been identified, being, dihydrochrysin (pinocembrin), 7-O-methyl
dihydrochrysin (pinostrobin), naringenin, 7-O-methyl naringenin (sakuranetin), 4'-Omethyl naringenin (isosakuranetin), 7,4'-O-dimethyl naringenin, eridictyol, 7-O-methyl
eriodictyol, 3’-O-methyl eriodictyol (padmatin) and 7, 3’-O-dimethyl eriodictyol (De
Pascual Teresa et al., 1986; 1984; González et al., 1983; Hurabielle et al., 1982; ValantVetschera et al., 2003).
Further studies confirmed the presence of 7-O-methyl aromadendrin, 7-O-methyl
taxifolin, 7, 3’-O-dimethyl taxifolin and 7, 4’-O-dimethyl taxifolin as the principal
dihydroflavonols (Hurabielle et al., 1982; Valant-Vetschera et al., 2003). In
A.
campestris subsp. maritima, the flavone 6-methoxyapigenin (hispidulin) and the
dihydroflavonol 7,3’-O-dimethyl taxifolin were isolated from the chloroformic extract
of the aerial part (Rauter et al. , 1989). However, the acetone and chloroformic extracts
seem to contain mainly the dihydroflavones: 5,8,4’-trihydroxyflavanone, 5,6-dihydroxy
4’-methoxyflavanone, naringenin, 7-O-methyl naringenin (sakuranetin), 4’-O-methyl
naringenin (isosakuranetin), eriodictyol, 7, 3’-O-dimethyl eriodictyol and 7, 4’-Odimethyl eriodictyol (Rauter et al., 1989; Vasconcelos et al. , 1998). About A.
campestris subsp. campestris, the flavonoid patterning was less complicated with the
only subclass of flavonols that was represented by the compounds 3,4’-O-dimethyl
kaempferol (ermanin), 3,4’-O-dimethyl 5’-methoxykaempferol and 3’-O-methyl
quercetin (isorhamnetin) found in the cyclohexane, ethyl acetate and dichloromethane
extracts of the aerial part (Ferchichi et al., 2006) (Fig. 2, 3 and 4: see supplementary
data on-line)).
5.6.
Phenolic acids
Riedel et al. (2010) found that the cells in culture obtained from seed germination of A.
13
campestris L. are rich in phenolic compounds such as chlorogenic acid, trans ferulic
acid, 4-methoxy-cinnamic acid, vanillic acid and isochlorogenic acids A, B and C.
These findings are in accordance with other studies conducted on phenolic rich extract
of A. campestris L. from Algeria and on methanolic, water and ethyl acetate extracts of
the aerial part of Tunisian A. campestris L. which found that these preparations
contained chlorogenic and caffeic acids as well as isochlorogenic acids A, B and C
(Djeridane et al., 2007; Megdiche-Ksouri et al., 2015; Sebai et al., 2014) (Fig.5). It is of
interest to note that phenolic content is highly sensitive to the measuring method
(Boulanouar et al., 2013), and the phenol content measured by Folin-Ciocalteu of the
Algerian A.campestris L. was lower than those reported by other authors (Djeridane et
al., 2007; Djidel and Khennouf, 2014; Karabegović et al., 2011; Megdiche-Ksouri et al.,
2015).
5.6.
Coumarins and isocoumarins
According to Naili et al. (2010) and Masotti et al. (2012), leaves of A. campestris L. do
not contain coumarins. However, other studies have clearly shown the existence of
coumarin and its derivatives in several extracts of A. campestris L. including
hydroxycoumarins, esculetin, iso-fraxidin, fraxidin, scopolin, herniarin, scopoletin
(González et al., 1983; Megdiche-Ksouri et al., 2015; Vasconcelos et al., 1998). In
addition, three isocoumarins were isolated from A. campestris subsp. campestris:
artemidinal, E-artemidin and (+) epoxyartemidin (Ferchichi et al., 2006) (Figure 5: see
supplementary data on- line).
5.6.
Other compounds
The hexane extract of the leaves of A. campestris L. showed a high amount of fatty
acids, which the most important are, linoleic acid α-linolenic acid and palmitic acid
14
(Carvalho et al. , 2011). Petunidin-3-O-acetyl glucoside was the first and only
anthocyanin contained in the methanolic extract of the aerial part of A.campestris L.
from Tunisia (Megdiche-Ksouri et al., 2015).
5.6.
Volatile compounds
The species A. campestris L. can be subdivided into several chemotypes, which can be
classified according to the variability of their volatile fractions, which vary between
different populations growing in various localities. The main components which
predominate in the essential oil and extracts of this plant seem to be common to almost
all the subspecies of A. campestris L. (Figure 6: see supplementary data on-line).
Tunisia:
The essential oil of the aerial part of A. campestris L., analyzed in 4 different localities
from Southern Tunisia and over different phenologic stages, has been found to be more
abundant in sesquiterpenes (66-93%), with a weaker content of monoterpenes (529.8%). The main terpenes found are: ß-pinene (24-49.8%), p-cymene (2.3-22.3%), αpinene (4.1-12.5%), camphor (10.3%), spathulenol (1.2-10%), γ-muurolene (0.5-9.6%),
limonene (4.9-9.3%), germacrene D (7.3 %), (ar)-curcumene (6.9%), α-cubebene
(6.6%), γ-terpinene (2.2-6.5%), β-eudesmol (1-6.4%), myrcene or β- myrcene (1.4-6%),
(Z)-ß-ocimene (1.8-5.5%), geranyl acetate (5%), (E)-ß-ocimene (4.3%) and (Z)-βfarnesene (2.9- 4.2%) (Akrout et al., 2011, 2010, 2007, 2003, 2001). Additional analysis
showed that essential oil of the subspecies glutinosa growing in Tunisia was rich in βpinene (41.1%), p-cymene (9.9%), α-terpinene (7.9%), (Z)-β-ocimene (6.7%), limonene
(6.5%) and myrcene (4.1%) (Aicha et al., 2008). Moreover, menthol and artemisinic
acid have been found in the ethyl acetate and methanolic extracts of A.campestris L.
15
from Tunisia (Megdiche-Ksouri et al., 2015).
Algeria
In Algeria, little variability has been found in essential oils obtained from A. campestris
L. growing in two different regions, which were rich in monoterpenes (84.5-91.7%),
while the total of sesquiterpenes were estimated as (5.1%-7.2%). The major components
of A. campestris L. have been identified as β-pinene (25.6%), α-Terpenyl acetate
(18.8%), α-pinene (18.4%), sabinene (17%), (Z, E)-farnesol (10.3%), camphor (9.2%),
camphene (7.7%), limonene (6.6%), cedrol (5.4%), borneol (5.2%), p-cymene (4.1%),
and verbenone (3.8%) (Belhattab et al. , 2011; Boulanouar et al., 2013; Dob et al. ,
2005).
Spain
In a study conducted by De Pascual Teresa et al. (1983), the hexane extract from the
leaves of the Spanish A. campestris L. contained the sesquiterpenes: phytol, spathulenol
and β-eudesmol.
Portugal
The analysis of A. campestris subsp. maritima essential oil from Portugal afforded the
identification of 31 terpenic compounds; the most abundant compounds were: β-pinene
(17.8%) and cadin-4-en-7-ol (16.4%), γ-terpinene (8.7%), cis-β-ocimene (7.4%),
aromadendrene (6.7%), δ-cadinene (5.1%) and limonene (4.2%) (Silva et al. , 2002;
Silvestre et al. , 1999).
France
In France, the comparison between the different phenological stages of A. campestris
16
subsp. glutinosa showed that the essential oil composition was quite similar between the
various stages; with as main components: γ-terpinene (20.8-46.5%), capillene (8.933.1%), 1-phenyl-2,4-pentadiyne (16.2-29.7%), spathulenol (11.3%), O-methyleugenol
(4.5-6.6%), 1-phenyl-2,4-pentadiynone (6%) and p-cymene (4.5%). However, it should
be noted that γ-terpinene was more abundant in the vegetative stage, while capillene
was more quantified during the seed stage (Juteau et al., 2002). Another screening of the
volatile composition of the ethanolic extract obtained from the aerial part of
A.campestris subsp. glutinosa occurring in France showed the presence of the major
components: capillene (49.1%), γ-terpinene (23%) and 1-phenylpenta-2,4-diyne
(18.1%) (Masotti et al., 2012).
Italy
In Italy, the essential oil composition of three varieties of A. campestris L. has been
determined. The first study reported the seasonal composition of A. campestris subsp.
glutinosa, and the major compounds characterized were β-pinene (6.9-57.2%),
germacrene D (5.9-28.6%), bicyclogermacrene (3.9- 14.5%), myrcene (3.8- 11.2%), αpinene (5.3-9.2%), α-bisabolol (3.2-7%), methyl eugenol (3.7-6.9%), limonene (46.7%), spathulenol (4.1-6.6%), viridiflorol (4.1-6.6%), (E,E)-α-farnesene (5-5.9%), (E)β-ocimene (4.9-5.4%) and α-humulene (4.8%) (Bellomaria et al., 2001). The second
analysis carried out on A. campestris subsp. borealis, highlighted the presence of the
caryophyllene oxide (18.2%) as the main component, followed by α-pinene (15.3%), βpinene (9.8%), spathulenol (9.3%), 2, 3-dihydro-1,8-cineole (5.2%), limonene (4.9%)
and pinocarveol (3.8%) (Mucciarelli et al. , 1995).
Poland
17
A comparative study of the volatiles contained in the essential oils of the different parts
of A. campestris subsp. campestris from Poland showed a new major compound (Z)falcarinol (19-38.8%), that mainly existed in the stem and roots, followed by
germacrene D (9.7-28%) in the inflorescences, flowers, leaves and stems parts. The
remaining major compounds: γ-humulene (4-8.2%), (E)-ß-caryophyllene (4.1-6.5%),
Germacra-4(15),5,10(14)-trien-1α-ol (4.2-5.5%), (E,E)-α-Farnesene (3.5-4.3%), were
similarly distributed in the inflorescences, flowers, stems and leaves. In this subspecies,
the sesquiterpenes predominate (38.7-76.7%) the monoterpenes (7-19.8%) (Lis and
Kowal, 2015).
Lithuania
The essential oil profiling of A. campestris subsp. campestris from different parts of
Lithuania showed different profiles of the essential oils with the presence of
caryophyllene oxide (3.7-38.8%), germacrene D (3.8-31.2%), γ-curcumene (4-14.8%),
β-pinene (3.9-13.8%), α-pinene (4-11.4%), humulene epoxide II (3.7-11.7%), β-silene
(6.5-10.8%), β-caryophyllene (3.8-10%), spathulenol (4-9.7%), (E,E)-α-farnesene (5.79.4%), β-ylangene (3.7-8.3%), β-elemene (3.8-7.6%), eudesma-4(15),7-dien-1β-ol (0.17.1%), junenol (4.6-6.1%), cis-pinane (6%), (Z)-β-farnesene (3.8-5.6%), α-cadinol (4.17.4%), germacra-4(15),5(10),14-trien-1α-ol (4-6.3%), α-humulene (5%), limonene
(5%), (E)-β-ocimene (4.5%), sabinene (4.2%), epi-α-muurolol (4.1%); δ-cadinene (0.23.8%). In the subspecies occurring in Poland, this essential oil is characterized by a
predominance of sesquiterpenes (41.1-79.3%) over the monoterpenes (5.4-19.7%)
(Judzentiene and Budiene, 2014; Judzentiene et al. , 2010).
Southern Ural
18
Another study in Southern Ural confirmed that the essential oil of A. campestris L. was
composed by α-pinene (41%), β-pinene (29.7%), limonene (6.4%) and sabinene (4.5%)
(Khalilov et al. , 2001).
Iran
From Iran, Kazemi et al. (2009) drew the comparative profile of the terpenic
compounds of three parts of A. campestris L.; accordingly, it has been found that the
flower and stem contain sub-equal levels of monoterpenes (48.1% and 45.8%,
respectively), and sesquiterpenes (7.3% and 18.2%, respectively), while the leaves own
60.1% of monoterpenes and 20.9% of sesquiterpenes; all the different parts however
contained spathulenol (15.8-29.2%), α-pinene (23-29.2%), β-pinene (4.5-12.6%),
bicyclogermacrene (9.1-12%), (Z)-ß-ocimene (3.2- 6.8%), germacrene D (5.3-6.6%),
limonene (3.4-6.3), p-cymene (4.8%) and (E)-ß-ocimene (3.9%).
Turkey
The Turkish essential oil of A. campestris L. aerial part appeared to have a different
chemical composition; the main constituents are:
tremetone (15.83%), capillin
(10.38%), spathulenol (6.47%), β-pinene (6.31%), methyl-eugenol (5.49%), α-thujone
(4.78%) and p-cymene (3.75%) (Baykan Erel et al. , 2012).
Serbia
The essential oil of A. campestris L. growing in Serbia contained approximately equal
quantity of monoterpenes (22.5%) and sesquiterpenes (20.8%). Also, it was poor in
volatile fractions and the major components found were spathulenol (9.2%) and βpinene (9.1%) (Chalchat et al., 2003).
19
6.
Pharmacology of Artemisia campestris L.
6.1 Antioxidant activity
6.1.1. DPPH (diphenyl picrylhydrazyl) radical scavenging activity
From Tunisia, the studies found that the antioxidant effect of methanolic extract of
shoots possesses the half maximal inhibitory concentration (IC50) of about 730µg/ml
(Tlili et al., 2013); while the methanolic extract of leaves and that of the shoots
expressed the respective values 22µg/ml and 6µg/ml, when compared to the standard
BHT (Butylated hydroxytoluene) (IC50=72µg/ml). The antioxidant provoked by
methanolic extract of shoots seems to be more efficient (El Abed et al. , 2014;
Megdiche-Ksouri et al., 2015). The aqueous extract of leaves possessed an IC50
equivalent to 160µg/ml, though, this radical scavenging activity is lesser than that of
ascorbic acid (IC50=72µg/ml) (Sebai et al., 2014). Otherwise, the leaf extract showed a
weak antioxidant effect (IC50>62µg/ml), if compared to that of ascorbic acid which
possess an IC50 ranging from 2 to 3.5µg/ml (Sefi et al. , 2013). Another report found
that the aqueous fraction from the aerial part expressed an antioxidant activity,
corresponding to the IC50: 27.5µg/ml; this value was two times higher than that of the
standard BHT (IC50=11.5µg/ml) (Megdiche-Ksouri et al., 2015). However the IC50 of
the ethyl acetate fraction was 10µg/ml, which was comparable to that displayed by BHT
(IC50=11.5µg/ml) (Megdiche-Ksouri et al., 2015). In regard to the essential oil of
leaves, the IC50 was about 1874µg/ml, comparatively to that of ascorbic acid
(IC50=2.5µg/ml), the observed activity is too low (Akrout et al., 2010); the same
observation was made with the antioxidant effect of essential oil from the aerial part,
that has an IC50=94500µg/ml, which is judged to be very feeble when compared to
20
standards: ascorbic acid (IC50=240µg/ml), quercetin (IC50= 280µg/ml) and BHT
(IC50=840µg/ml) (Akrout et al., 2011). From Algeria, the essential oil and the phenolic
extracts obtained from the leaves and the fruits of A. campestris L. showed an important
scavenging activity against DPPH radical, and the IC50 was found to be equal to
39µg/ml; this activity appear to be more effective referring to the standard BHT
(IC50=89µg/ml) (Bakchiche and Gherib, 2014). Also, the ethyl acetate extract of the
aerial part showed a radical scavenging activity estimated by an IC50=58µg/ml; yet, this
effect is lower by comparison to the standard rutin (Djidel and Khennouf, 2014).
Karabegović et al. (2011) tested the antioxidant effect of three methanolic extracts
recovered by different extraction techniques from the aerial parts of A. campestris L.
collected in Bulgaria; as result, it has been asserted that the extracts have relatively the
same scavenging potential toward DPPH radical, with an IC50 ranging from 19.8 to 23
µg/ml.
6.1.2. ABTS+ (2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) free radical
scavenging activity
The phenolic extract from the aerial part of A. campestris L. occurring in Algeria, has
been found to possess an IC50= 25mmol Trolox equivalent/g of extract dry weight,
which corresponds to the value 573µg/g of dry matter (2006; Djeridane et al., 2007).
Otherwise, the phenolic extract from the leaves and fruits gave an antioxidant effect,
that corresponds to the IC50=15µg/ml. BHT, used as standard reference, showed higher
antioxidant effect, regarding the IC50=4µg/ml) (Bakchiche and Gherib, 2014). Another
study showed that the scavenging potential of this radical by aqueous extract was much
lower and antioxidant activity was estimated by an IC50=26000µg/ml (Barkat et al. ,
2014). According to other reports, it has been demonstrated that the ABTS scavenging
21
activity was estimated to be equivalent to 1737, 1000 and 77µmol Trolox equivalent/g
of extract, successively for the aqueous-ethanolic extract, the infusion and the essential
oil. These scavenging activities are considered interesting
by comparison to the
standard BHT that displayed IC50=6513µmol Trolox equivalent/g of extract (Akrout et
al., 2011), while the value 16mg Trolox equivalent/g of extract dry weight corresponded
to the radical scavenging power reported for the methanolic extract of the shoots (Tlili
et al., 2013).
6.1.3. Other in-vitro antioxidant effects
The ability of A. campestris L. extracts of preventing the lipid peroxidation by
inhibition of β-carotene bleaching have been tested. Consequently, ethyl acetate extract
produced 82% of bleaching inhibition and exceeded that of BHT; similarly, chloroform
extract also inhibited 79% of β-carotene bleaching (Djidel and Khennouf, 2014).
Furthermore, It has been reported that the aqueous extract, the aqueous-ethanolic extract
and the essential oil obtained from the aerial part of A. campestris L., showed
differential antioxidant effects on the β-carotene/linoleic acid system, with the most
pronounced activity of the aqueous extract (88.7%) which was comparable to that of the
standard BHT (89%). However this elevated antioxidant effect of the extract is related
to the high concentration used which is 9.6 times higher than that of the essential oil and
the hydro-alcoholic extract (Akrout et al., 2011). Moreover, many extracts of A.
campestris L. have been tested on many reactive oxygen species; thus, ethyl acetate and
chloroform extracts showed a high scavenging activity of hydroxyl radicals with 0.17
and 0.22 mg/ml respectively, even though, this activity is lower than that of the vitamin
C (Djidel and Khennouf, 2014). Otherwise, the aqueous extract of the aerial part
inhibited 75% of the hydroxyl radical and showed 95% of peroxide anion scavenging
22
activity (Aniya et al., 2000), while the concentration 47.5 mg/ml inhibited 50% of the
oxidation produced by the superoxide anion (Saoudi et al. , 2010). The crude extract and
the aqueous extract of A.campestris L. aerial part afforded 100% of ferrous ion
chelating activity (Djidel and Khennouf, 2014). Additional studies about the ferric
reducing antioxidant power of the methanolic extract, the aqueous and the ethyl acetate
fractions of the aerial part of the plant exhibited the respective efficient doses: 110, 230
and 340µg/ml, which was far from that of the standard ascorbic acid (IC50=37µg/ml)
(Megdiche-Ksouri et al., 2015). Additionally, ethyl acetate and chloroform extracts of
the aerial part increased the reducing power, with respective efficient doses: 45 and
170µg/ml, still the electron donation capacity of the extracts remain inferior to that of
the standard BHT (Djidel and Khennouf, 2014). The phenolic extracts from the aerial
part, at different concentrations have been assayed for their capacity to protect human
blood against the free radical aggression. Consequently, the concentration 10-4M of the
phenolic extract produced 208% of inhibition of the hemolysis, while the concentration
30µM inhibited only 50% of the hemolysis (Djeridane et al. , 2010; 2007). One more
test allowed to measure the total antioxidant activity by mean of measuring the reducing
power of phosphomolybdenum blue by methanolic extract of leaves (55mg ascorbic
acid equivalent/g of dry weight) (El Abed et al., 2014), which appear to be much weaker
once compared to methanolic extract of shoots (IC50=540mg gallic acid equivalent/g dry
weight), aqueous fraction (IC50=216mg gallic acid equivalent/g dry weight) and ethyl
acetate fraction (IC50=328mg gallic acid equivalent/g dry weight) (Megdiche-Ksouri et
al., 2015)
6.2. Antibacterial activity
Recently, many studies have been carried out with the aim of highlighting the capacity
23
of many extracts of A. campestris to prevent the growth of bacterial strains. Methanolic
extract seems to exert remarkable antibacterial effect on many bacterial strains and with
various zone of inhibition. El Abed et al. (2014) and Naili et al. (2010) showed a
significant antibacterial effect of the methanol extract of leaves of A. campestris against
a wide range of bacteria such as Escherichia coli (17 and 10mm), Bacillus cereus
(25mm), Bacillus subtilis (21 to 32mm), Staphylococcus aureus (20 and 27mm),
Salmonella enteritidis and Salmonella typhi (13 and 8mm), Pseudomonas aeruginosa
(9mm) compared to the effects of chloramphenicol (20 -33mm), streptomycin (1222mm) and ceftazidime (12-27mm). Similarly examined the effect of two types of
methanol extracts of the aerial part against seven strains of bacteria; the best action was
obtained against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and
Pseudomonas aeruginosa, with the zones of inhibition ranging from 18 to 21mm; these
effects appear to be higher than those obtained with erythromycin (20-20.5mm) and
tylosin Tartarat (17-18mm). On the contrary, other authors reported little or no
antibacterial effect of methanolic extract or compounds isolated from methanolic extract
of the aerial part of A.campestris (Baykan Erel et al., 2012; Megdiche-Ksouri et al.,
2015; Tharib et al. , 1983) compared to standard antibiotics. Also, the ethyl acetate
fraction from the aerial part exhibited significant levels of inhibition against Bacillus
thuringiensis (18.3mm), Listeria monocytogene (13.5mm), Escherichia coli (13 mm),
Aeromonas hydrophila (12mm), Vibrio parahaemolyticus (9mm), Staphylococcus
aureus (9mm), Vibrio alginolyticus (10mm), Vibrio cholerac (11mm) and Vibrio
vulnificus (10.5mm) strains, but, this effect is considered weak compared to the
examined standards: gentamicine (15-46 mm) and chloramphenicol (8-30 mm)
(Megdiche-Ksouri et al., 2015). Moreover, the essential oil displayed varying
24
magnitude of inhibition patterns with Escherichia coli (18-20mm), Pseudomonas
aeruginosa (18mm) and Staphylococcus aureus (10.5-14mm), the results seem to be
close to those found with the antibiotics tested: ceftazidine and gentamicine (14-22 mm)
(Akrout et al., 2010; 2007; Baykan Erel et al., 2012; Ghorab et al. , 2013). However, the
acetone and the aqueous extracts possessed relatively weak activity towards the
microorganisms: Staphylococcus aureus (7.7-13mm), Vibrio parahaemolyticus (10mm),
Staphylococcus epidermidis (10mm), Staphylococcus saprophiticus (10mm), Listeria
monocytogene (8.5mm), Escherichia coli (7 mm) and Salmonella typhimirium (7mm),
when compared to antibiotics: oxacillin, tetracyclin, chloramphenicol, streptomycin,
and gentamicine (Ben Sassi et al., 2007; El Abed et al., 2014).
6.3 Antifungal activity
A promising antifungal efficiency for several extracts of A. campestris L. against many
fungal species has been evidenced. When tested on the strains Trichophyton tonsurans,
Trichophyton rubrum and Microsporum canis, the aqueous extract of A. campestris
induced 100% of growth inhibition; the same result was obtained with standards
voriconazole, fluconazole, itraconazole and amphotericin B (Webster et al. , 2008).
Against Candida glabrata, Candida parapsilosis and Candida albicans, the same
extract produced 7 mm as zone of inhibition; again, this effect is quite similar to that of
amphotericin B (8.4-10mm) (Megdiche-Ksouri et al., 2015). Moreover, the methanolic
extract of stems has been noted to possess differential degrees of antifungal activity
revealed by different percentages of inhibition against Fusarium oxysporum (54%),
Aspergillus
fumigatus
(47%),
Fusarium
verticillioides
(39%),
Penicillium
brevicompactum (31%) and Aspergillus flavus (30%) (Zabka et al. , 2011); also, the
methanolic extract of this plant blocked the growth of Aspergillus niger and provoked a
25
zone of inhibition about 32.5-33 mm, which is more important than erythromycin (20.5
mm) and tylosin tartarat (18 mm) (Karabegović et al., 2011). Whereas, the zone of
inhibition ranging from 7 to 9 mm has been observed after the treatment of Candida
glabrata, Candida parapsilosis and Candida krusei and Candida albicans colonies with
the methanol and ethyl acetate extracts from the aerial part (Megdiche-Ksouri et al.,
2015); this antifungal effect is close to that exerted by the standard amphotericin B .
Quite simply, the antifungal effect of the essential oil has been restricted, only, on the
variety Trichophyton longifusus, for which the growth inhibition was 65%; the same
antifungal effect has been found with miconazole (70% of growth inhibition) (Akrout et
al., 2007).
6.4
Insecticidal activity
Nowadays, the search for botanical pesticides creates great interest, due to their minor
toxic effects on the environment and humans. In this regard, further research about the
insecticidal activity of A. campestris L. has been undertaken. It has been found that the
methanolic extract of its stem displayed the highest larvicidal activity, with 100%
mortality of Culex quinquefasciatus (mosquito larvae), and the appraised value of the
LD50 was approximately 23 parts per million (Pavela, 2009). However, the larval
mortality induced by the ethanolic extract was quite mild, and killed only 33.6% of the
Culex pipiens L. mosquito larvae (Masotti et al., 2012). However, the lifespan of the
insects Spodoptera littoralis and Bruchus obtectus were moderately reduced, in
response to the treatment with the essential oil, with an average inhibition of 50%
(Akrout et al., 2007). Another research study indicated different larvicidal efficiency,
represented mainly by the repellency effect on Tribolium castaneum larvae after 2-24
hours of exposure to both hexane and acetone extracts from the aerial part of the plant
26
(Pascual-Villalobos and Robledo, 1998).
6.5. Anthelmintic activity
Helminthiasis represent one of the major constraints that livestock producers meet;
Also, it is well known that A. campestris L. is an abundant pastoral species especially in
arid regions. In this respect, ethanolic and aqueous extracts of this plant have been
tested in-vitro for their anthelmintic activity, by using the sheep parasite Haemonchus
contortus. Both extracts, at 2 mg/ml, provoked the total inhibition of egg hatching;
moreover, after 24 hours of exposure, 100% worm’s mortality had been achieved in the
presence of the ethanolic extract at 2mg/ml, while, the same concentration of the
aqueous extract killed 70 % of worms (Akkari et al., 2014).
6.6 Antitumor activity
The anti-mutagenicity effects are potentially useful as an antitumoral treatment; the
anti-mutagenic potential of the essential oil from aerial part of A. campestris has been
assessed on two Salmonella typhimurium strains after induction of mutagenicity caused
by the incorporation of the carcinogen benzo-[a]-pyrene. As result, the inhibitory
percentages 87.3% and 73.2% have been noted, respectively in the presence of the
S.typhimurium TA97 and S. typhimurium TA98 assay systems at a dose of 100 µg of the
oil/plate (Aicha et al., 2008).
Despite all the studies and advances in cancer research, the plant species are regarded as
promising sources of new anticancer agents with low toxicity against non-tumoral cells.
Many investigations reported the cytotoxic effect of extracts from A. campestris L.
against several types of cancerous cells using the colorimetric MTT assay (3-(4,5methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), for assessing the cell viability.
27
When compared to a negative control growth, essential oil of the aerial part of A.
campestris L. at 100 µg/ml exhibited 80% of inhibition of HT-29 human
adenocarcinoma cell line, followed by the infusion and the hydro-ethanolic extract
which showed respectively 87% and 43% of the control growth of HT-29 cells (Akrout
et al., 2011), while the hexanic and chloroform extracts demonstrated a weak anticancer
profile against the cervix adenocarcinoma (Hela cells) with the correspondent
percentage of inhibition 26% and 28%. The chloroform extract showed a less
pronounced antiproliferative affect against A431 cells (skin epidermoid carcinoma) with
19% of inhibition, similarly, the methanolic extract, inhibited only, 15% of the MCF7
breast carcinoma cells (Réthy et al. , 2007). An important dose-response cytotoxic
effect has been detected with the doses ranging from 25 to 3200 µg/ml of the ethanolic
extract on the Hep-2 (human Caucasian hepatocyte carcinoma) and HepG-2 cells
(human Caucasian larynx carcinoma) (Vahdati-Mashhadian et al. , 2009). Nonetheless,
the concentration range from 25 to 1000µg/ml of the methanolic extract tested on MCF7
and A549 cancerous cell lines and on A7R5 and 293T normal cell lines expressed a
very low cytotoxic effect (Erel et al. , 2011).
6.7. Antihypertensive effect
According to the clinical trial conducted on 14 adult smoker and non-smoker
volunteers, diastolic pressure and heart rate were decreased after 30 – 45mins in both
groups after taking a decoction of A. campestris L. leaves at 20 mg/ml. without
affecting the mean blood pressure of the latter non-hypertensive group. The percent of
men with pre diagnosed hypertension in the cigarette smoking group decreased from 50
to 33%, after one hour from taking the water boiled extract. Whilst this only
demonstrated an immediate response, this result showed the potential of Artemisia
28
extract to offset hypertension. (Ben-Nasr et al. , 2014).
7Toxicology of Artemisia campestris L.
Two acute toxicity tests of A. campestris L. aqueous extract of leaves, collected in
Tunisia have been evaluated on mice. After 24 hours following the intraperitoneal
administration of five different doses (1, 2, 3, 4, 5 g/kg) of the extract, general
depression and abdominal constriction have been observed at doses higher than 3 g/kg
body weight, and the mean value of the median lethal dose (LD50) has been equivalent
to 2.5 g/kg of body weight (Sefi et al 2010). However, after the oral administration of
the doses 0.0125, 0.025, 0.05, 0.1, 0.2, 0.4, 0.8, 1.6 and 3.2 g/kg of the aqueous extract
neither abnormal behavior nor mortality has been detected during the observation
period. Thus, the LD50 value was greater than 3.2 g/kg body weight (Sebai et al. 2014).
Moreover, toxicity of A. campestris ssp. campestris essential oils from Lithuania has
been determined using the brine shrimp (Artemia sp.) assay. The test showed that
lethality (LD50) of brine shrimp larvae was about 15 to 20 µg/ml (Judzentiene et al.,
2010). On the other hand, marked signs of intoxications, like the irritation in the
digestive tracts and diarrhea besides to a proteinuria and hematuria, have been observed
when large amounts of young growth of A. campestris L. have been ingested by
dromedaries and goats in Tunisia (El Bahri et al. , 1997).
8 Conclusion and perspectives
In this review, we report the results of works carried out on A. campestris L., which
provide practical support for further research that can be undertaken in the future.
Pharmacological studies listed in this document show almost all ethnomedicinal uses of
this herb, including anthelmintic, anticancer, antifungal and antimicrobial and many
29
other applications. Concerning the chemical composition of the different parts of A.
campestris L., it can be clearly concluded that this species has a variable phytochemical
profile, which can possibly justify its bioactive potential. However, the lack of bioguided isolation strategies is unfortunate since all bioassays so far on A. campestris L.
concern only the crude extracts or essential oils. Therefore, it would be interesting to
explore the path to isolate and purify the chemical components that may be biologically
active. In addition, the mechanism of action, the bioavailability and pharmacokinetics of
isolated pure compounds will have the greatest interest in the valuation of the obtained
pharmacological effect. Another relevant feature that can also contribute to the
development of this plant is its use in clinical practice; to date, there is a huge shortage
in this regard; therefore, clinical studies are needed to confirm the relevance of its
traditional use.
Conflict of interest
None declared.
Acknowledgements
We are grateful to Mr. A. Berrichi, Professor in Faculté des Sciences (Département de
Biologie, Université Mohammed Premier, Oujda-Maroc) for his huge efforts in the care
and maintenance of the plant A. campestris L. growing in his experimental station
within the Faculty. We are also thankful to Mr. A. Berraaouan, PhD student and
member of ―Laboratoire de Physiologie, Génétique et Ethnopharmacologie‖ URAC-40,
(Département de Biologie, Faculté des Sciences, Université Mohammed Premier,
Oujda-Maroc) for the high quality of A. campestris L. photos taken in the experimental
station.
30
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42
Table 1 - Ethnomedicinal use of Artemisia campestris L.
Country
Common name
Part used
Mode of
Traditional uses
References
preparation
Morocco
Algeria
Allal, chih lakhrissi
Leaves,
Decoction,
Antidiabetic, treatment of eye
(Bnouham et al., 2002; El
flowers, seeds
infusion,
diseases, melisma and for
Hassani et al., 2013;
poultice
digestive, respiratory and allergic
Fakchich and Elachouri,
problems
2014)
Taguq, tguft, degoufet,
Leaves, stems,
Decoction,
Treatment of cutaneous and
(Benchelah et al., 2004;
tadjuq, tedjok, alala,
fruits, aerial
infusion,
gastro-intestinal problems,
Boudjelal et al., 2013;
hellala, tamemmayt,
part
powder,
analeptic, anthelmintic,
Boulanouar et al., 2013;
poultice
antidiabetic, antihypertensive,
Djidel et al., 2009; Ferchichi
diuretic, vulnerary, circulation
et al., 2006; Gast, 1989;
regulator, febrifuge, vermifuge,
Hammiche and Maiza, 2006)
um nefsa
emmenagogue and for postpartum care, analeptic
Tunisia
Dguft , tgouft
Leaves,
Decoction
aerialpart
Antivenom, anti-inflammatory,
(Ben Sassi et al., 2007;
anti-rheumatic, anthelmintic, anti-
Leporatti and Ghedira, 2009;
eczema and cutaneous issues,
43
treatment of fever, cough, urinary
Tlili et al., 2013)
infections and digestive problems;
used for fever and cough and as a
tonic
Libya
sc’ahâl, togoft, tegoft,
taghert, tâghiat,
teghoch
Spain
escoba de río,
Flowers,
NS
Anthelmintic
(De Natale and Pollio, 2012)
Flowers
Decoction
Treatment for baldness
(Benítez et al., 2010)
NS
NS
Antiulcer, and febrifuge
(Guarino et al., 2008;
leaves
mojariega, tomillo,
granilloPeganoSalsoletea
Italy
Tammarice
Leporatti and Ghedira, 2009)
Serbia
NS
Leaves
Antihelminthic, antiseptic,
NS
(Popović et al., 2012)
emmenagogue, tonic, nervine
Iran
Berenjasf (common
NS
Anticancer
NS
name to some
44
(Naghibi et al. , 2014)
Artemisia sp.)
India
Nagdona (common
Leaves
Antivenom
NS
name to some
(Bahekar et al., 2012;
Kapoor and Saraf, 2011)
Artemisia sp.)
Japan
Ryukyuyomogi
NS
NS
Treatment of liver, kidney and
(Aniya et al., 2000; Minami
diabetes disorders and for
et al., 2010)
jaundice,
Argentina
Alcanfor
USA and
Field wormwood, field
Canada
sagewort
Leaves, stems
Leaves, roots
Infusion,
Treatment for cough, bronchitis,
(Kujawska and Hilgert,
maceration
and contusions.
2014)
Poultice, tea,
Abortifacient, respiratory,
(Shemluck, 1982)
infusion
cutaneous conditions, digestive
problems
NS:
not
45
specified