Issue 17, 2009
From the journal:

Lab on a Chip

Electrophoretic separation of DNA in gels and nanostructures†

G. B. Salieb-Beugelaar,*a   K. D. Dorfman,b   A. van den Berga  and  J. C. T. Eijkel*a  

* Corresponding authors

a BIOS/Lab-on-a-Chip Group, Mesa+ Institute for Nanotechnology, University of Twente, P.O. Box 217, Enschede, AE, The Netherlands
E-mail: j.c.t.eijkel@utwente.nl, g.b.salieb-beugelaar@ewi.utwente.nl
Tel: 31-53-489-5653

b Department of Chemical Engineering and Materials Science, University of Minnesota - Twin Cities, 421 Washington Ave. S.E., Minneapolis, MN, USA

Abstract

The development of nanostructure devices has opened the door to new DNA separation techniques and fundamental investigations. With advanced nanotechnologies, artificial gels (e.g. nanopillar arrays, nanofilters) can be manufactured with controlled and ordered geometries. This contrast with gels, where the pores are disordered and the range of available pore sizes is limited by the level of cross-linking and the mechanical properties of the gel. In this review, we recall the theories developed for free-solution and gel electrophoresis (extended Ogston model, biased reptation and entropic trapping) and from this perspective, suggestions for future concepts for fast DNA separation using nanostructures will be given.

Graphical abstract: Electrophoretic separation of DNA in gels and nanostructures

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Article information

DOI
https://doi.org/10.1039/B905448K
Article type
Critical Review
Submitted
17 Mar 2009
Accepted
01 May 2009
First published
11 Jun 2009

Lab Chip, 2009,9, 2508-2523

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Electrophoretic separation of DNA in gels and nanostructures

G. B. Salieb-Beugelaar, K. D. Dorfman, A. van den Berg and J. C. T. Eijkel, Lab Chip, 2009, 9, 2508 DOI: 10.1039/B905448K

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