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Anti-Acetyllysine Mouse mAb
Catalog Number: PTM-101
$ 330

Clone Number: Kac-01

Host: Mouse Clonality: Monoclonal

Applications: WB IHC-P IP

Reactivity: All

Synonyms: Kac

Product Size
100 μl
Quantity

Shipping: Ambient temperature

Order online or send purchase order to info@ptmbio.com

FAQ Technical Support Protocols

General Information
Isotype IgG
Conjugate Unconjugated
Synonyms Kac
UniProt ID

/

Immunogen Acetylated lysine peptides
MW (kDa) Multiple
Specificity Anti-Acetyllysine Mouse mAb detects proteins with acetylated lysine residues. This pan antibody recognizes acetylated lysine independent of its surrounding sequences.
Product Usage Information
Applications Dilution Recommended Species
WB 1:500 - 1:1000 All
IHC-P 1:500 - 1:2000 Human, Mouse
IP 1:25 - 1:300 Human
Properties
Purity Protein G and immunogen affinity purified
Constituents PBS, Glycerol, BSA
Storage Store at -20°C. Avoid freeze/thaw cycles.
Stability Stable for 12 months from date of receipt/reconstitution.
Target Information

Background

The reversible lysine acetylation of histones and non-histone proteins is a crucial post-translational modification that regulates diverse cellular processes, including chromatin dynamics, gene expression, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 histone deacetylases (HDACs) have been identified, but the mechanisms governing substrate selection and site specificity of these enzymes remain unclear. Dysregulation of protein acetylation has been implicated in the development of cancers and other diseases, and HDACs have become likely targets for anti-cancer drugs.

Cellular location

/

Images
Dot Blot

Protein amount: 4 ng, 20 ng, 100 ng, 500 ng
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
The list of proteins used in the experiment is provided below.
Lane 1: Acetylated BSA. Lane 2: Propionylated BSA. Lane 3: Butyrylated BSA.
Lane 4: Unmodified BSA.

WB

Lysates: HeLa cells
Protein loading amount: 20 μg
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: (A) PTM-101+BSA, (B) PTM-101+acetylated BSA, 2 hours at room temperature
Secondary Ab: Goat Anti- Mouse IgG H&L pAb (HRP Conjugate)
Exposure time: 60 seconds
Predicted band size: Multiple
Observed band size: Multiple

IHC-P

Tissue: Mouse cerebrum
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Description: Color brown is the positive signal for PTM-101

Tissue: Human liver
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Description: Color brown is the positive signal for PTM-101

Tissue: Human testis
Section type: Formalin-fixed & paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 1 hour at room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (blue)
Description: Color brown is the positive signal for PTM-101

IP

IP of HeLa+Sodium butyrate (30mM, 4hr) cells extracts
IP ab incubation condition: PTM-101, 4°Covernight, 1:25, 1:100, 1:300 dilution
WB primary ab incubation condition: (Left) PTM-116, (Right) PTM-107, room temperature 2h, 1:1000 dilution
Secondary ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Left: Anti-H3K27ac rabbit pAb (PTM-116)
Right: Anti-H2BK5ac rabbit pAb (PTM-107)
Observed MW: 17 kDa/14 kDa
Exposure time: 30 s

Research Use

For research use only, not for use in diagnostic procedures.

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References (61)
  • Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner
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  • Lysine 2-Hydroxyisobutyrylation- and Succinylation-Based Pathways Act Inside Chloroplasts to Modulate Plant Photosynthesis and Immunity
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  • The effect and mechanism of Zigui-Yichong-Fang on improving ovarian reserve in premature ovarian insufficiency by activating SIRT1/Foxo3a pathway
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  • SIRT3-and FAK-mediated acetylation-phosphorylation crosstalk of NFATc1 regulates Nε-carboxymethyl-lysine-induced vascular calcification in diabetes mellitus
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    Authors Mingjuan Gu, et al.
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    Authors Doudou Tan, et al.
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    Reactivity
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    Applications Unspecified
    Reactivity
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    Year 2020
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    Authors Min Zheying, et al.
    Applications Unspecified
    Reactivity
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    Year 2020
    Journal Frontiers in Microbiology
    Authors Li Hailong, et al.
    Applications Unspecified
    Reactivity
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    Year 2020
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    Authors Yang Qianqian, et al.
    Applications Unspecified
    Reactivity
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    Year 2019
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    Authors Zhang Di, et al.
    Applications Unspecified
    Reactivity
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    Year 2019
    Journal Nature Metabolism
    Authors Liu Zuojun, et al.
    Applications Unspecified
    Reactivity
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    Year 2019
    Journal Communications Biology
    Authors Li Yanjian, et al.
    Applications Unspecified
    Reactivity
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    Year 2019
    Journal HUMAN REPRODUCTION
    Authors Cheng Yi min, et al.
    Applications Unspecified
    Reactivity
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    Year 2019
    Journal MOLECULAR & CELLULAR PROTEOMICS
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    Applications Unspecified
    Reactivity
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    Year 2019
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    Authors Qiangzhen Yang, et al.
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    Year 2019
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    Authors Yufang Tang, et al.
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    Year 2018
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    Authors He Huang, et al.
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    Year 2018
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    Authors Huang He, et al.
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    Year 2018
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    Authors Jun Yu Xu, et al.
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