Abstract
Plastid transformation for the expression of recombinant proteins and entire enzymatic pathways has become a promising tool for plant biotechnology in the past decade. Several improvements of the technology have turned plant plastids into robust and dependable expression platforms for multiple high value compounds. In this chapter, we describe our current methodology based on Gateway® recombinant cloning, which we have adapted for plastid transformation. We describe the steps required for cloning, biolistic transformation, identification, and regeneration of transplastomic plant lines and Western blot analysis.
The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-3289-4_20
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-3289-4_20
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References
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Acknowledgements
This work was supported by the GLOBVAC program (Project 192510), the Research Council of Norway (RCN), Bioforsk.
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Gottschamel, J., Lössl, A. (2016). Chloroplast-Based Expression of Recombinant Proteins by Gateway® Cloning Technology. In: MacDonald, J., Kolotilin, I., Menassa, R. (eds) Recombinant Proteins from Plants. Methods in Molecular Biology, vol 1385. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3289-4_1
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DOI: https://doi.org/10.1007/978-1-4939-3289-4_1
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3288-7
Online ISBN: 978-1-4939-3289-4
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