Abstract
This study investigated the potential use of acid-fermented chicken silage on growth performance, digestive enzymes, liver and intestinal histology in Oreochromis mossambicus (Mozambique tilapia) (Peters 1852). Five silage-based diets were formulated by replacing fishmeal at 0% (control), 25%, 50%, 75% and 100%, thereafter denoted as D1, D2, D3, D4 and D5, respectively. The control diet (0%) contained 40% of fishmeal while D5 contained no fishmeal. The diets contained the same protein (30.06%), fat (15%) and energy levels (18 MJ kg−1). Silage (29.31% protein and 10.97% fat) fed alone and a commercial feed (35% protein and 2.97% fat) were designated as D6 and D7, respectively. Each diet was randomly assigned to triplicate groups of O. mossambicus (6.06 ± 0.27 g) stocked in 400-L fiberglass tanks (50 fish/tank). Fish were fed with the diets to apparent satiation twice daily for 45 days. Among the silage-based diets, weight gained was significantly higher (P < 0.05) in D4 (18.34 ± 1.51 g) and D5 (20.86 ± 1.95 g) than in D1 (14.28 ± 1.21 g), D2 (14.19 ± 1.51 g) and D3 (14.43 ± 1.34 g). The highest specific growth rates were found in D4 (1.10 ± 0.19%) and D5 (1.23 ± 0.04%) than in D1 (0.96 ± 0.22%), D2 (0.74 ± 0.06%) and D3 (0.92 ± 0.04%). The best feed conversion ratio was found in D4 (2.55 ± 0.27) and D5 (2.41 ± 0.32) compared to D1 (3.13 ± 0.38), D2 (3.50 ± 0.34) and D3 (3.26 ± 0.42). Apparent digestibility coefficient for protein values was higher in D4 (93.50 ± 2.96%) and D5 (92.55 ± 3.90%) than in the control (86.68 ± 2.80%). However, the best growth rates were recorded in the D6 and D7 diets. The highest protease, amylase and lipase activities were recorded in D6 and D7 followed by D5 and D4. No pathological alterations were observed in the liver and intestines of fish among dietary treatments. This study confirmed that acid-fermented chicken silage could serve as an alternative and cheaper protein source, as well as complete diet for O. mossambicus.
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Acknowledgements
The authors would like express their heartfelt gratitude to Mr. Nell Wiid, a fish farmer who helped to collect the chicken carcasses and process it on his farm. We are also grateful to honours students at ARU who assisted during fish sampling and dissections. We are equally thankful to the anonymous reviewers whose criticism and guidance led to an improved final product.
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The Department of Forestry, Fisheries and the Environment provided funds for this study.
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Chris Henri Fouche and Esau M. Mbokane conceived the study and wrote the manuscript. Esau M. Mbokane and Lucia Matlale Mbokane carried out the experiments, collected data, analyzed and interpreted it.
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This study is part of a multi-year project focusing on the use of locally-available plant and animal waste resources in an effort to develop cheaper and high-quality diets for freshwater fish species in South Africa. Ethical clearance was granted by the University of Limpopo Animal Research Ethics Committee (AREC) of the Faculty of Science and Agriculture. Handling and sacrificing of fish was executed in a humane manner, and international, national and/or institutional guidelines governing the use of animals were observed at all times.
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Mbokane, E.M., Mbokane, L.M. & Fouche, C.H. The effect of fishmeal replacement with acid-fermented chicken silage on growth, digestive enzyme activity and histology of the intestine and liver of juvenile Mozambique tilapia (Oreochromis mossambicus). Aquacult Int 30, 2491–2512 (2022). https://doi.org/10.1007/s10499-022-00916-5
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DOI: https://doi.org/10.1007/s10499-022-00916-5