Abstract
Corynebacterium striatum, a common constituent of the human skin microbiome, is now considered an emerging multidrug-resistant pathogen of immunocompromised and chronically ill patients. However, little is known about the molecular mechanisms in the transition from colonization to the multidrug-resistant (MDR) invasive phenotype in clinical isolates. This study performed a comprehensive pan-genomic analysis of C. striatum, including isolates from “normal skin microbiome” and from MDR infections, to gain insights into genetic factors contributing to pathogenicity and multidrug resistance in this species. For this, three novel genome sequences were obtained from clinical isolates of C. striatum of patients from Brazil, and other 24 complete or draft C. striatum genomes were retrieved from GenBank, including the ATCC6940 isolate from the Human Microbiome Project. Analysis of C. striatum strains demonstrated the presence of an open pan-genome (α = 0.852803) containing 3816 gene families, including 15 antimicrobial resistance (AMR) genes and 32 putative virulence factors. The core and accessory genomes included 1297 and 1307 genes, respectively. The identified AMR genes are primarily associated with resistance to aminoglycosides and tetracyclines. Of these, 66.6% are present in genomic islands, and four AMR genes, including aac(6')-ib7, are located in a class 1-integron. In conclusion, our data indicated that C. striatum possesses genomic characteristics favorable to the invasive phenotype, with high genomic plasticity, a robust genetic arsenal for iron acquisition, and important virulence determinants and AMR genes present in mobile genetic elements.
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Data availability
The genome sequences generated in this study are deposited in NCBI’s GenBank and can be accessed through the following IDs: GCA_002865925.1; GCA_002775055.1; GCA_002775105.1.
References
Abe M, Kimura M, Maruyama H et al (2021) Clinical characteristics and drug susceptibility patterns of Corynebacterium species in bacteremic patients with hematological disorders. Eur J Clin Microbiol Infect Dis 15–17. https://doi.org/10.1007/s10096-021-04257-8
Alibi S, Ferjani A, Boukadida J et al (2017) Occurrence of Corynebacterium striatum as an emerging antibiotic-resistant nosocomial pathogen in a Tunisian hospital. Sci Rep 7:9704. https://doi.org/10.1038/s41598-017-10081-y
Alibi S, Ramos-Vivas J, Ben Selma W et al (2021) Virulence of clinically relevant multidrug resistant Corynebacterium striatum strains and their ability to adhere to human epithelial cells and inert surfaces. Microb Pathog 155:104887. https://doi.org/10.1016/j.micpath.2021.104887
Alikhan N-F, Petty NK, Ben Zakour NL, Beatson SA (2011) BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons. BMC Genomics 12:402. https://doi.org/10.1186/1471-2164-12-402
Allen CE, Schmitt MP (2009) HtaA is an iron-regulated hemin binding protein involved in the utilization of heme iron in Corynebacterium diphtheriae. J Bacteriol 191:2638–2648
Arndt D, Grant JR, Marcu A et al (2016) PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res 44:W16-21. https://doi.org/10.1093/nar/gkw387
Asgin N, Otlu B (2020) Antimicrobial resistance and molecular epidemiology of Corynebacterium striatum isolated in a tertiary hospital in Turkey. Pathogens 9:136. https://doi.org/10.3390/pathogens9020136
Bankevich A, Nurk S, Antipov D et al (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477
Barraud O, Badell E, Denis F et al (2011) Antimicrobial drug resistance in Corynebacterium diphtheriae mitis. Emerg Infect Dis 17:2078–2080. https://doi.org/10.3201/eid1711.110282
Bertelli C, Laird MR, Williams KP et al (2017) a. IslandViewer 4: expanded prediction of genomic islands for larger-scale datasets. Nucleic Acids Res 45:W30–W35. https://doi.org/10.1093/nar/gkx343
Billington SJ, Esmay PA, Songer JG, Jost BH (2002) Identification and role in virulence of putative iron acquisition genes from Corynebacterium pseudotuberculosis. FEMS Microbiol Lett 208:41–45
Biscarini S, Colaneri M, Mariani B et al (2021) A case of Corynebacterium striatum endocarditis successfully treated with an early switch to oral antimicrobial therapy. Infez Med 29:138–144
Bläckberg A, Falk L, Oldberg K et al (2021) Infective endocarditis due to Corynebacterium species: clinical features and antibiotic resistance. Open Forum Infect Dis 8:1–6. https://doi.org/10.1093/ofid/ofab055
Blin K, Shaw S, Kloosterman AM et al (2021) antiSMASH 6.0: improving cluster detection and comparison capabilities. Nucleic Acids Res 49:W29–W35. https://doi.org/10.1093/nar/gkab335
Brettin T, Davis JJ, Disz T et al (2015) RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci Rep 5:8365. https://doi.org/10.1038/srep08365
Camacho C, Coulouris G, Avagyan V et al (2009) BLAST+: architecture and applications. BMC Bioinforma 10:421. https://doi.org/10.1186/1471-2105-10-421
Carattoli A, Zankari E, García-Fernández A et al (2014) In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob Agents Chemother 58:3895–3903. https://doi.org/10.1128/AAC.02412-14
Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552
Chang W-T, Chen Z-C (2020) Pulsatile strangulation of the aorta - a rare presentation and etiology of infective endocarditis -. Circ J 84:1607. https://doi.org/10.1253/circj.CJ-20-0495
Changkaew K, Utrarachkij F, Siripanichgon K et al (2014) Characterization of antibiotic resistance in Escherichia coli isolated from shrimps and their environment. J Food Prot 77:1394–1401. https://doi.org/10.4315/0362-028X.JFP-13-510
Chaudhari NM, Gupta VK, Dutta C (2016) BPGA- an ultra-fast pan-genome analysis pipeline. Sci Rep 6:24373. https://doi.org/10.1038/srep24373
Chevreux B, Wetter T, Suhai S (1999) Genome sequence assembly using trace signals and additional sequence information. In: German conference on bioinformatics. Citeseer, pp 45–56
Clark NC, Olsvik Ø, Swenson JM et al (1999) Detection of a streptomycin/spectinomycin adenylyltransferase gene (<em>aadA</em>) in <em>Enterococcus faecalis</em>. Antimicrob Agents Chemother 43:157 LP – 160. https://doi.org/10.1128/AAC.43.1.157
Couvin D, Bernheim A, Toffano-Nioche C et al (2018) CRISPRCasFinder, an update of CRISRFinder, includes a portable version, enhanced performance and integrates search for Cas proteins. Nucleic Acids Res 46:W246–W251
Darwin KH (2009) Prokaryotic ubiquitin-like protein (Pup), proteasomes and pathogenesis. Nat Rev Microbiol 7:485–491. https://doi.org/10.1038/nrmicro2148
Delcher AL, Bratke KA, Powers EC, Salzberg SL (2007) Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 23:673–679. https://doi.org/10.1093/bioinformatics/btm009
de Souza C, Faria YV, de SantAnna LO et al (2015) Biofilm production by multiresistant Corynebacterium striatum associated with nosocomial outbreak. Mem Inst Oswaldo Cruz 110:242–248. https://doi.org/10.1590/0074-02760140373
de Souza C, Mota HF, Faria YV et al (2020) Resistance to antiseptics and disinfectants of planktonic and biofilm-associated forms of Corynebacterium striatum. Microb Drug Resist 26:1546–1558
de Souza C, Simpson-Louredo L, Mota HF et al (2019) Virulence potential of Corynebacterium striatum towards Caenorhabditis elegans. Antonie Van Leeuwenhoek 112:1331–1340. https://doi.org/10.1007/s10482-019-01265-9
Dorella FA, Pacheco LGC, Oliveira SC et al (2006) Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence. Vet Res 37:201–218. https://doi.org/10.1051/vetres:2005056
Dussurget O, Timm J, Gomez M et al (1999) Transcriptional control of the iron-responsive fxbA gene by the mycobacterial regulator IdeR. J Bacteriol 181:3402–3408. https://doi.org/10.1128/JB.181.11.3402-3408.1999
Edgar RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26:2460–2461. https://doi.org/10.1093/bioinformatics/btq461
El Sayed ZM, Mostafa Mahmoud N, Anies Rizk M (2022) Molecular study of integrase gene I and integrase gene II in clinical isolates of Pseudomonas aeruginosa. Infect Disord - Drug Targets 22:925. https://doi.org/10.2174/1871526522666220408111454
Galimand M, Fishovitz J, Lambert T et al (2015) AAC(3)-XI, a new aminoglycoside 3- N -acetyltransferase from Corynebacterium striatum. Antimicrob Agents Chemother 59:5647–5653. https://doi.org/10.1128/AAC.01203-15
Garcia CM, McKenna J, Fan L, Shah A (2020) Corynebacterium striatum bacteremia in end-stage renal disease: a case series and review of literature. R I Med J 103:46–49
Gaspar AH, Ton-That H (2006) Assembly of distinct pilus structures on the surface of Corynebacterium diphtheriae. J Bacteriol 188:1526–1533. https://doi.org/10.1128/JB.188.4.1526-1533.2006
Gomez M, Doukhan L, Nair G, Smith I (1998) sigA is an essential gene in Mycobacterium smegmatis. Mol Microbiol 29:617–628
Guindon S, Dufayard J-F, Lefort V et al (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307–321
Hahn WO, Werth BJ, Butler-Wu SM, Rakita RM (2016) Multidrug-resistant Corynebacterium striatum associated with increased use of parenteral antimicrobial drugs. Emerg Infect Dis 22:1908–1914. https://doi.org/10.3201/eid2211.160141
Hollnagel K, Willen J, Ellis M et al (2020) Chronic Corynebacterium striatum septic arthritis in a patient referred for total knee arthroplasty. Case Rep Orthop 2020:1–5. https://doi.org/10.1155/2020/1392182
Hsiao W, Wan I, Jones SJ, Brinkman FSL (2003) IslandPath: aiding detection of genomic islands in prokaryotes. Bioinformatics 19:418–420. https://doi.org/10.1093/bioinformatics/btg004
Hudson CM, Lau BY, Williams KP (2015) Islander: a database of precisely mapped genomic islands in tRNA and tmRNA genes. Nucleic Acids Res 43:D48–D53. https://doi.org/10.1093/nar/gku1072
Huerta-Cepas J, Forslund K, Coelho LP et al (2017) Fast genome-wide functional annotation through orthology assignment by eggNOG-Mapper. Mol Biol Evol 34:2115–2122. https://doi.org/10.1093/molbev/msx148
Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23:254–267
Jia B, Raphenya AR, Alcock B et al (2017) CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database. Nucleic Acids Res 45:D566–D573. https://doi.org/10.1093/nar/gkw1004
Kang SJ, Choi SM, Choi JA et al (2018) Factors affecting the clinical relevance of Corynebacterium striatum isolated from blood cultures. PLoS ONE 13:1–12. https://doi.org/10.1371/journal.pone.0199454
Katoh K, Rozewicki J, Yamada KD (2017) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 20:1160–1166. https://doi.org/10.1093/bib/bbx108
Khan D, Shadi M, Mustafa A et al (2021) A wolf in sheep’s clothing; case reports and literature review of Corynebacterium striatum endocarditis. Idcases 24:e01070. https://doi.org/10.1016/j.idcr.2021.e01070
Kline KA, Dodson KW, Caparon MG, Hultgren SJ (2010) A tale of two pili: assembly and function of pili in bacteria. Trends Microbiol 18:224–232
Konstantinidis KT, Tiedje JM (2005) Genomic insights that advance the species definition for prokaryotes. Proc Natl Acad Sci USA 102:2567–2572. https://doi.org/10.1073/pnas.0409727102
Krismer B, Weidenmaier C, Zipperer A, Peschel A (2017) The commensal lifestyle of Staphylococcus aureus and its interactions with the nasal microbiota. Nat Rev Microbiol 15:675–687. https://doi.org/10.1038/nrmicro.2017.104
Langille MGI, Hsiao WWL, Brinkman FSL (2008) Evaluation of genomic island predictors using a comparative genomics approach. BMC Bioinformatics 9:329. https://doi.org/10.1186/1471-2105-9-329
Leyton-Carcaman B, Abanto M (2022) Beyond to the stable: role of the insertion sequences as epidemiological descriptors in Corynebacterium striatum. Front Microbiol
Leyton B, Ramos JN, Baio PVP et al (2021) Treat me well or will resist: uptake of mobile genetic elements determine the resistome of Corynebacterium striatum. Int J Mol Sci 22:7499
Li M, Chen YL (1986) Structural studies on rhodilunancins A and B. J Antibiot (tokyo) 39:430–436. https://doi.org/10.7164/antibiotics.39.430
Liu B, Zheng D, Jin Q et al (2018) VFDB 2019: a comparative pathogenomic platform with an interactive web interface. Nucleic Acids Res 47:D687–D692. https://doi.org/10.1093/nar/gky1080
Liu B, Zheng D, Zhou S et al (2022) VFDB 2022: a general classification scheme for bacterial virulence factors. Nucleic Acids Res 50:D912–D917. https://doi.org/10.1093/nar/gkab1107
Liu W, Xie Y, Ma J et al (2015) IBS: an illustrator for the presentation and visualization of biological sequences. Bioinformatics 31:3359–3361. https://doi.org/10.1093/bioinformatics/btv362
Manabe YC, Hatem CL, Kesavan AK et al (2005) Both Corynebacterium diphtheriae DtxR(E175K) and Mycobacterium tuberculosis IdeR(D177K) are dominant positive repressors of IdeR-regulated genes in M. tuberculosis. Infect Immun 73:5988–5994. https://doi.org/10.1128/IAI.73.9.5988-5994.2005
Mandlik A, Swierczynski A, Das A, Ton-That H (2008) Pili in Gram-positive bacteria: assembly, involvement in colonization and biofilm development. Trends Microbiol 16:33–40. https://doi.org/10.1016/j.tim.2007.10.010
Mansour MK, Al-Messabi AH, Ahmed SA et al (2020) Corynebacterium striatum prosthetic valve endocarditis. A case report and literature review. Clin Infect Pract 7–8:100055. https://doi.org/10.1016/j.clinpr.2020.100055
Mattos-Guaraldi AL, Guimarães LC, Santos CS et al (2015) Draft genome sequence of Corynebacterium striatum 1961 BR-RJ/09, a multidrug-susceptible strain isolated from the urine of a hospitalized 37-year-old female patient. Genome Announc 3:e00869-e915. https://doi.org/10.1128/genomeA.00869-15
Mistry J, Finn RD, Eddy SR et al (2013) Challenges in homology search: HMMER3 and convergent evolution of coiled-coil regions. Nucleic Acids Res 41:e121–e121. https://doi.org/10.1093/nar/gkt263
Mitchell KF, McElvania E, Wallace MA et al (2021) Evaluating the rapid emergence of daptomycin resistance in Corynebacterium : a multicenter study. J Clin Microbiol 59:1–12. https://doi.org/10.1128/JCM.02052-20
Moura A, Soares M, Pereira C et al (2009) INTEGRALL: a database and search engine for integrons, integrases and gene cassettes. Bioinformatics 25:1096–1098. https://doi.org/10.1093/bioinformatics/btp105
Navas J, Fernández-Martínez M, Salas C et al (2016) Susceptibility to aminoglycosides and distribution of aph and aac(3)-XI genes among Corynebacterium striatum clinical isolates. PLoS ONE 11:e0167856. https://doi.org/10.1371/journal.pone.0167856
Néron B, Littner E, Haudiquet M et al (2022) IntegronFinder 2.0: identification and analysis of integrons across bacteria, with a focus on antibiotic resistance in Klebsiella. Microorg 10
Nofiani R, de Mattos-Shipley K, Lebe KE et al (2018) Strobilurin biosynthesis in Basidiomycete fungi. Nat Commun 9:3940. https://doi.org/10.1038/s41467-018-06202-4
Nudel K, Zhao X, Basu S et al (2018) Genomics of Corynebacterium striatum, an emerging multidrug-resistant pathogen of immunocompromised patients. Clin Microbiol Infect 24:1016.e7-1016.e13. https://doi.org/10.1016/j.cmi.2017.12.024
Otsuka Y, Ohkusu K, Kawamura Y et al (2006) Emergence of multidrug-resistant Corynebacterium striatum as a nosocomial pathogen in long-term hospitalized patients with underlying diseases. Diagn Microbiol Infect Dis 54:109–114. https://doi.org/10.1016/j.diagmicrobio.2005.08.005
Paulsen IT, Brown MH, Skurray RA (1996) Proton-dependent multidrug efflux systems. Microbiol Rev 60:575 LP – 608
Pereira Baio PV, Franceschi Mota H, D’avila Freitas A et al (2013) Clonal multidrug-resistant Corynebacterium striatum within a nosocomial environment, Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz 108:23–29. https://doi.org/10.1590/S0074-02762013000100004
Proft T, Baker EN (2009) Pili in Gram-negative and Gram-positive bacteria—structure, assembly and their role in disease. Cell Mol Life Sci 66:613
Qian Y, Lee JH, Holmes RK (2002) Identification of a DtxR-regulated operon that is essential for siderophore-dependent iron uptake in Corynebacterium diphtheriae. J Bacteriol 184:4846–4856. https://doi.org/10.1128/JB.184.17.4846-4856.2002
Qin Q-L, Xie B-B, Zhang X-Y et al (2014) A proposed genus boundary for the prokaryotes based on genomic insights. J Bacteriol 196:2210–2215. https://doi.org/10.1128/JB.01688-14
Racewicz P, Majewski M, Biesiada H et al (2022) Prevalence and characterisation of antimicrobial resistance genes and class 1 and 2 integrons in multiresistant Escherichia coli isolated from poultry production. Sci Rep 12:1–13
Rai AK, Mitchell AM (2020) Enterobacterial common antigen: synthesis and function of an enigmatic molecule. Mbio 11:e01914-e1920. https://doi.org/10.1128/mBio.01914-20
Raman S, Hazra R, Dascher CC, Husson RN (2004) Transcription regulation by the <em>Mycobacterium tuberculosis</em> alternative sigma factor SigD and its role in virulence. J Bacteriol 186:6605 LP – 6616. https://doi.org/10.1128/JB.186.19.6605-6616.2004
Ramos JN, Baio PVP, Veras JFC et al (2022) Novel configurations of type IE CRISPR-Cas system in Corynebacterium striatum clinical isolates
Ramos JN, Rodrigues IDS, Baio PVP et al (2018) Genome sequence of a multidrug-resistant Corynebacterium striatum isolated from bloodstream infection from a nosocomial outbreak in Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz 113:e180051–e180051. https://doi.org/10.1590/0074-02760180051
Ramos JN, Souza C, Faria YV et al (2019) Bloodstream and catheter-related infections due to different clones of multidrug-resistant and biofilm producer Corynebacterium striatum. BMC Infect Dis 19:672. https://doi.org/10.1186/s12879-019-4294-7
Ramsey MM, Freire MO, Gabrilska RA et al (2016) Staphylococcus aureus shifts toward commensalism in response to Corynebacterium species. Front Microbiol 7. https://doi.org/10.3389/fmicb.2016.01230
Rasmussen M, Mohlin AW, Nilson B (2020) From contamination to infective endocarditis—a population-based retrospective study of Corynebacterium isolated from blood cultures. Eur J Clin Microbiol Infect Dis 39:113–119. https://doi.org/10.1007/s10096-019-03698-6
Renom F, Garau M, Rubí M et al (2007) Nosocomial outbreak of Corynebacterium striatum infection in patients with chronic obstructive pulmonary disease. J Clin Microbiol 45:2064–2067. https://doi.org/10.1128/JCM.00152-07
Roberts MC (2005) Update on acquired tetracycline resistance genes. FEMS Microbiol Lett 245:195–203. https://doi.org/10.1016/j.femsle.2005.02.034
Roberts MC, Sutcliffe J, Courvalin P et al (1999) Nomenclature for macrolide and macrolide-lincosamide-streptogramin B resistance determinants. Antimicrob Agents Chemother 43:2823–2830. https://doi.org/10.1128/AAC.43.12.2823
Rocha DJP, Azevedo V, Brenig B et al (2020) Whole-genome sequencing reveals misidentification of a multidrug-resistant urine clinical isolate as Corynebacterium urealyticum. J Glob Antimicrob Resist 23:16–19. https://doi.org/10.1016/j.jgar.2020.07.020
Rodriguez-R LM, Konstantinidis KT (2014) Bypassing cultivation to identify bacterial species: culture-independent genomic approaches identify credibly distinct clusters, avoid cultivation bias, and provide true insights into microbial species. Microbe Mag 9:111–118
Ruan Z, Feng Y (2015) BacWGSTdb, a database for genotyping and source tracking bacterial pathogens. Nucleic Acids Res 44:D682–D687. https://doi.org/10.1093/nar/gkv1004
Sadiya P, Bishai WR, Murphy JR (2019) Corynebacterium diphtheriae: diphtheria toxin, the tox operon, and its regulation by Fe2+ activation of apo-DtxR. Microbiol Spectr 7(4):7–4. https://doi.org/10.1128/microbiolspec.GPP3-0063-2019
Schmitt MP (2014) Iron acquisition and iron-dependent gene expression in Corynebacterium diphtheriae. In: Corynebacterium diphtheriae and Related Toxigenic Species. Springer, pp 95–121
Schröder J, Maus I, Meyer K et al (2012) Complete genome sequence, lifestyle, and multi-drug resistance of the human pathogen Corynebacterium resistens DSM 45100 isolated from blood samples of a leukemia patient. BMC Genomics 13:141. https://doi.org/10.1186/1471-2164-13-141
Singh N, Kumar A (2015) Virulence factor SenX3 is the oxygen-controlled replication switch of Mycobacterium tuberculosis. Antioxid Redox Signal 22:603–613. https://doi.org/10.1089/ars.2014.6020
Souza C (2021) Biofilm production on different abiotic surface, positive and negative charge by multi-drug resistance Corynebacterium striatum strains. Open Access J Surg 12:114–128. https://doi.org/10.19080/OAJS.2021.12.555843
Sreevatsan S, Pan X, Stockbauer KE et al (1996) Characterization of rpsL and rrs mutations in streptomycin-resistant Mycobacterium tuberculosis isolates from diverse geographic localities. Antimicrob Agents Chemother 40:1024–1026. https://doi.org/10.1128/AAC.40.4.1024
Suh JW, Ju Y, Lee CK et al (2019) Molecular epidemiology and clinical significance of Corynebacterium striatum isolated from clinical specimens. Infect Drug Resist 12:161–171. https://doi.org/10.2147/IDR.S184518
Swierczynski A, Ton-That H (2006) Type III pilus of corynebacteria: pilus length is determined by the level of its major pilin subunit. J Bacteriol 188:6318–6325. https://doi.org/10.1128/JB.00606-06
Tauch A, Burkovski A (2015) Molecular armory or niche factors: virulence determinants of Corynebacterium species. FEMS Microbiol Lett 362:fnv185. https://doi.org/10.1093/femsle/fnv185
Tauch A, Krieft S, Kalinowski J, Pühler A (2000) The 51,409-bp R-plasmid pTP10 from the multiresistant clinical isolate Corynebacterium striatum M82B is composed of DNA segments initially identified in soil bacteria and in plant, animal, and human pathogens. Mol Gen Genet MGG 263:1–11. https://doi.org/10.1007/PL00008668
Tauch A, Zheng Z, Pühler A, Kalinowski J (1998) Corynebacterium striatum chloramphenicol resistance transposon Tn5564: genetic organization and transposition in Corynebacterium glutamicum. Plasmid 40:126–139. https://doi.org/10.1006/plas.1998.1362
Tettelin H, Masignani V, Cieslewicz MJ et al (2005) Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial “pan-genome.” Proc Natl Acad Sci 102:13950–13955
Tettelin H, Riley D, Cattuto C, Medini D (2008) a Comparative genomics: the bacterial pan-genome. Curr Opin Microbiol 11:472–477. https://doi.org/10.1016/j.mib.2008.09.006
Timms VJ, Hassan KA, Mitchell HM, Neilan BA (2015) Comparative genomics between human and animal associated subspecies of the Mycobacterium avium complex: a basis for pathogenicity. BMC Genomics 16:695. https://doi.org/10.1186/s12864-015-1889-2
Trost E, Ott L, Schneider J et al (2010) The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence. BMC Genomics 11:728. https://doi.org/10.1186/1471-2164-11-728
Verroken A, Bauraing C, Deplano A et al (2014) Epidemiological investigation of a nosocomial outbreak of multidrug-resistant Corynebacterium striatum at one Belgian university hospital. Clin Microbiol Infect off Publ Eur Soc Clin Microbiol Infect Dis 20:44–50. https://doi.org/10.1111/1469-0691.12197
Waack S, Keller O, Asper R et al (2006) Score-based prediction of genomic islands in prokaryotic genomes using hidden Markov models. BMC Bioinforma 7:142. https://doi.org/10.1186/1471-2105-7-142
Wang J, Wang Y, Du X et al (2016) Rapid transmission of multidrug-resistant Corynebacterium striatum among susceptible patients in a tertiary hospital in China. J Infect Dev Ctries 10:1299–1305. https://doi.org/10.3855/jidc.7577
Wang J, Ge P, Qiang L et al (2017) The mycobacterial phosphatase PtpA regulates the expression of host genes and promotes cell proliferation. Nat Commun 8(1):244. https://doi.org/10.1038/s41467-017-00279-z
Wang X, Zhou H, Chen D et al (2019) Whole-genome sequencing reveals a prolonged and persistent intrahospital transmission of Corynebacterium striatum, an emerging multidrug-resistant pathogen. J Clin Microbiol 57:e00683-e719. https://doi.org/10.1128/JCM.00683-19
Wang X, Zhou H, Du P et al (2021) Genomic epidemiology of Corynebacterium striatum from three regions of China: an emerging national nosocomial epidemic. J Hosp Infect 110:67–75. https://doi.org/10.1016/j.jhin.2020.10.005
Wattam AR, Davis JJ, Assaf R et al (2017) Improvements to PATRIC, the all-bacterial Bioinformatics Database and Analysis Resource Center. Nucleic Acids Res 45:D535–D542. https://doi.org/10.1093/nar/gkw1017
Wright GD, Thompson PR (1999) Aminoglycoside phosphotransferases: proteins, structure, and mechanism. Front Biosci 4:D9-21
Zhang M-J, Cao X-J, Fan J et al (2020) Corynebacterium striatum meningitis combined with suspected brain and lung abscesses: a case report and review. BMC Infect Dis 20:389. https://doi.org/10.1186/s12879-020-05114-3
Zulauf KE, Sullivan JT, Braunstein M (2018) The SecA2 pathway of Mycobacterium tuberculosis exports effectors that work in concert to arrest phagosome and autophagosome maturation. PLoS Pathog 14:e1007011–e1007011. https://doi.org/10.1371/journal.ppat.1007011
Acknowledgements
HNRJ was the recipient of a Ph.D scholarship from FAPESB. LGCP was the recipient of a research fellowship from CNPq.
Funding
This study was partially supported by grants from FAPESB, CNPq, CAPES, and FINEP in Brazil, through the following funding schemes: Fundação de Amparo à Pesquisa do Estado da Bahia—BOL0505/2018 and JCB17/2013; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES-PROCAD 071/2013; Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq Nº 09/2018; and Financiadora de Estudos e Projetos, MCT/FINEP/CT-INFRA01/2013.
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H.N.R.J., J.N.R., D.J.P.G.R., D.A.A., C.S.S., and J.V.O.C.: investigation, formal analysis, methodology, software, data curation, visualization, writing—original draft; H.N.R.J., C.S., and L.S.S: investigation, formal analysis, methodology, visualization, writing—review and editing; V.V.V., J.N., R.T.J.R, V.A., E.R.G.R.A., A.L.M-G., and L.G.C.P: conceptualization, funding, resources, project administration, supervision, writing—review and editing.
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Jesus, H.N.R., Ramos, J.N., Rocha, D.J.P.G. et al. The pan-genome of the emerging multidrug-resistant pathogen Corynebacterium striatum. Funct Integr Genomics 23, 5 (2023). https://doi.org/10.1007/s10142-022-00932-x
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DOI: https://doi.org/10.1007/s10142-022-00932-x