Abstract
Acinetobacter indicus strain UBT1 has shown efficient lipase (243 U ml−1) and biosurfactant (61.1% E24% emulsification and surface tension reduction to 37.7 mN m−1) production capabilities using agro-industrial waste as sole carbon source. We report here the draft genome sequence of A. indicus strain UBT1 having genome size of 2.97 Mb with 45.90% GC content. Total 2721 coding genes were predicted using National Center for Biotechnology Information-Prokaryotic Genome Annotation Pipeline (NCBI-PGAP). The whole genome shotgun project sequence data are accessible through NCBI Gene Bank under accession no. JABFOI000000000. PGAP annotation revealed the presence of the triacylglycerol lipase, phospholipase etc., that circuitously confers the oil consumption competency to the strain UBT1. Rapid Annotation using the Subsystem Technology (RAST) server used for mapping the genes to the subsystem resulted in 278 subsystem with 30% subsystem coverage. The draft genome data can be used to exploit the A. indicus strain UBT1 for its advance biotechnological application and also for further comparative genomic studies.
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The support received by the Department of Biosciences as CAS-SAP from UGC, New Delhi, India, is duly acknowledged.
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RP contribute to isolation, microbiological work, methodology, and genome analysis. RS performed isolation and genome analysis. VP was involved in genome analysis, bioinformatics, and formal analysis. KP helped with conceptualization, writing, reviewing, and editing. UT contributed to conceptualization and bioinformatics.
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284_2021_2380_MOESM2_ESM.file
(FILE 3 kb) Cluster assemblies: Cluster of assemblies were generated with removing the redundancy of considered assemblies. Similar assemblies are forming one cluster and have only a single representative chosen based on assembly N50 (Cluster_accessions file lists the cluster name, followed by a tab, followed by a comma-delimited list of the assemblies in that cluster, with the representative assembly marked with a *)
284_2021_2380_MOESM3_ESM.file
(FILE 226 kb) Distance matrix: FastANI produces pairwise ANI measurements of all the generated assemblies’ cluster using only the sequence shared by two assemblies
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Patel, R.K., Shah, R.K., Prajapati, V.S. et al. Draft Genome Analysis of Acinetobacter indicus Strain UBT1, an Efficient Lipase and Biosurfactant Producer. Curr Microbiol 78, 1238–1244 (2021). https://doi.org/10.1007/s00284-021-02380-5
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DOI: https://doi.org/10.1007/s00284-021-02380-5