Cynomolgus/Rhesus macaque B7-H5/Gi24/VISTA Protein (BH7-CM175)
Catalog: BH7-CM175
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| Product Description | Recombinant Cynomolgus/Rhesus macaque B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe33-Thr192. |
Product Background |
B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. |
Product Category |
Recombinant Protein |
Target |
B7-H5/Gi24/VISTA |
Gene/Protein ID |
XP_005565644.2/F7F6S6 |
Species |
Cynomolgus/Rhesus macaque |
Exact Sequence |
Phe33-Thr192 |
| Molecular Weight | The protein has a predicted MW of 19.13 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Cynomolgus/Rhesus macaque B7-H5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus/Rhesus macaque B7-H5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. |
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The purity of Cynomolgus/Rhesus macaque B7-H5 is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus/Rhesus macaque B7-H5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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