VPS4A Antibody (A-11): sc-393428

VPS4A Antibody (A-11) is an IgG2b κ mouse monoclonal VPS4A antibody that detects VPS4A of mouse, rat and human origin by WB, IP, IF and ELISA. VPS4A Antibody (A-11) is available as the non-conjugated anti-VPS4A antibody form, as well as multiple conjugated forms of anti-VPS4A antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Class E vacuolar protein sorting (vps) proteins are necessary for the appropriate sorting of receptors in the yeast endocytic pathway. Yeast Vps4p is a member of the AAA protein family (ATPases associated with diverse cellular activities) and plays an important role in transporting proteins out of prevacuolar endosomal compartments. In humans, two non-allelic orthologous proteins, VPS4A and VPS4B, are known and can form heteromeric complexes with each other. Both VPS4A and VPS4B are class E VPSs and are involved in intracellular protein trafficking, similar to Vps4p in yeast. HIV-1 uses cellular machinery to bud from infected cells and requires the two human VPS4 proteins and tsg 101 (tumor susceptibility gene 101) for this budding process. Dominant negative mutants of VPS4 proteins inhibit vacuolar protein sorting and also arrest HIV-1 and MLV budding. Thus, retroviruses normally use the VPS pathway to form multivesicular bodies during the budding process.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes.  |  Bishop, N. and Woodman, P. 2001. J Biol Chem. 276: 11735-42. PMID: 11134028
2. CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins.  |  Howard, TL., et al. 2001. J Cell Sci. 114: 2395-404. PMID: 11559748
3. Mammalian cells express two VPS4 proteins both of which are involved in intracellular protein trafficking.  |  Scheuring, S., et al. 2001. J Mol Biol. 312: 469-80. PMID: 11563910
4. Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.  |  Garrus, JE., et al. 2001. Cell. 107: 55-65. PMID: 11595185
5. Resistance is futile: assimilation of cellular machinery by HIV-1.  |  Perez, OD. and Nolan, GP. 2001. Immunity. 15: 687-90. PMID: 11728331
6. Comparative sequence and expression analyses of four mammalian VPS4 genes.  |  Beyer, A., et al. 2003. Gene. 305: 47-59. PMID: 12594041
7. The role of the VPS4A-exosome pathway in the intrinsic egress route of a DNA-binding anticancer drug.  |  Chen, VY., et al. 2006. Pharm Res. 23: 1687-95. PMID: 16841193
8. Ubiquitin depletion and dominant-negative VPS4 inhibit rhabdovirus budding without affecting alphavirus budding.  |  Taylor, GM., et al. 2007. J Virol. 81: 13631-9. PMID: 17913808
9. ESCRT-III recognition by VPS4 ATPases.  |  Stuchell-Brereton, MD., et al. 2007. Nature. 449: 740-4. PMID: 17928862

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VPS4A Antibody (A-11) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-VPS4A Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VPS4A Antibody (A-11) Specifications

Alternate Product Name

VPS4A

Product Clone Name

A-11

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2b kappa light chain

Amino Acids

349-370

Epitope Target

VPS4A

Epitope Species

human

Product Reactivity/Detection

VPS4A

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

8 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG_2b BP-HRP: sc-542741 (Mouse IgG_2b binding protein conjugated to horseradish peroxidase)
• m-IgG_2b BP-B: sc-542744 (Mouse IgG_2b binding protein conjugated to biotin)
• m-IgG_2b BP-FITC: sc-542743 (Mouse IgG_2b binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2b BP-CFL 488: sc-542745 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 488)
• m-IgG_2b BP-CFL 555: sc-542746 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 555)
• m-IgG_2b BP-CFL 594: sc-542747 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 594)
• m-IgG_2b BP-CFL 647: sc-542748 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 647)
• m-IgG_2b BP-CFL 680: sc-542749 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 680)
• m-IgG_2b BP-CFL 790: sc-542750 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 790)
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Mouse IgG Kappa Light Chain Binding Proteins
• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)



Available Conjugates/Forms




AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin




Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-VPS4A Antibody (A-11) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), ELISA (Enzyme-Linked Immunosorbent Assay).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

The preferred VPS4B antibody is VPS4B Antibody (A-11): sc-377162.