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Smad2/3 Antibody (C-8) - Western Blotting - Image 59787

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Smad2/3 Antibody (C-8): sc-133098. Western blot analysis of Smad2/3 expression in K-562 (A), U-937 (B), KNRK (C) and NIH/3T3 (D) whole cell lysates.

Smad2/3 Antibody (C-8): sc-133098

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Datasheets
  • Smad2/3 Antibody (C-8) is a mouse monoclonal IgG2a κ, cited in 110 publications, provided at 200 µg/ml
  • raised against amino acids 1-425 representing full length Smad3 of human origin
  • Anti-Smad2/3 Antibody (C-8) is recommended for detection of Smad2 and Smad3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine
  • Anti-Smad2/3 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-133098 X, 200 µg/0.1 ml)
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Smad2/3 (C-8): sc-133098.
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Smad2/3 Antibody (C-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Smad2/3 Antibody (C-8) (see ordering information below).

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Smad2/3 Antibody (C-8) is an IgG2a κ mouse monoclonal Smad2/3 antibody that detects Smad2 and Smad3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Smad2/3 Antibody (C-8) is available as the non-conjugated anti-Smad2/3 antibody form, as well as multiple conjugated forms of anti-Smad2/3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

I want to detect SMAD2/3 phosphorylated protein expression in porcine cells. But I'm not found this criteria in primary antibody of Santa cruz. I found that p-Smad2/ 3 (Ser 423/425) is not available now. Can you suggest me which one is proper?

Asked by: Ploy
Thank you for your question. Unfortunately we do not have a anti-phosphorylated SMAD antibody at this time. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-20

I WOULD LIKE TO PURCHASE ANTIBODY AGAINST PHOSPHORLYATED SMAD1 AN SMAD2 AND THEIR SECONDARY ANTIBODY FOR WESTERN BLOT

Asked by: MM ALHARBI
Thank you for your question. Unfortunately, we do not currently offer any phospho-specific Smad1 or Smad2 antibodies. This mouse monoclonal, Smad2/3 Antibody (C-8): sc-133098, is against total Smad2/3 and may detect both the phosphorylated and non-phosphorylated forms of the proteins.
Answered by: Technical Support
Date published: 2017-08-22

Can Smad2/3 (C-8): sc-133098 monoclonal antibody be used for detection of Smad2/3 of equine origin?

Asked by: jenni
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 14
Date published: 2017-01-11
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Rated 5 out of 5 by win7 from Smad 2/3 I tested this antibody in western blotting (1:200) and I am very satisfied.
Date published: 2024-01-26
Rated 5 out of 5 by HJJ326 from This antibody is very good! The bands bound with this antibody are very well exposed. I purchased this antibody last year and performed western blot detection on the cell protein samples collected from the RIPA lysate, and the background was very clean.
Date published: 2022-10-08
Rated 5 out of 5 by CYMF from The antibody was very good When I use the antibody, the bands were easy to visualize and very clear.
Date published: 2022-07-29
Rated 4 out of 5 by Anonymous from Good in immunofluorescence Good resuts have been achieved in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA.
Date published: 2020-11-10
Rated 4 out of 5 by gra15 from Buena marca Obtuve buena marca, debo corregir el inespecifico o diluir el acpo
Date published: 2019-03-14
Rated 5 out of 5 by IuriCV from Nice and strong band (w.b.) Produced a single band at the expected molecular weight (~55kDa). Sample: MDA-MB-231 wcl (30ug). Blocking: 1h in blocking buffer (0.05% tween-20 in TBS with 5% skin milk). Primary antibody: 1:200 in blocking buffer, overnight @ 4oC. Secondary antibody: 1:3000 in blocking buffer. Exposure time: 5 seconds* I'm certainly very satisfied with this product. *further dilution of the primary antibody, maybe even at 1:1000, will likely yield similar results at the expense of a prolonged (and more adequate) exposure time.
Date published: 2018-11-30
Rated 5 out of 5 by mingwunl from my review for Smad2/3 antibody (sc-133098) I tested a free antibody for Smad2/3 antibody (sc-133098) using IP samples from mouse fibrotic kidneys for Western blot. We loaded 80 ug proteins in each slot in the SDS gel. The antibody was diluted in 1:500 in 3% BSA/PBST and incubated overnight with the blot. We can observe a very clear band around 55-60 kd. We have repeated this experiment several times and used the fresh diluted antibody on the fresh blot.
Date published: 2018-05-14
Rated 5 out of 5 by Erik from western blot Specific band was obtained and the background was very clear. Very good.
Date published: 2017-12-05
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Smad2/3 Antibody (C-8) is rated 4.8 out of 5 by 41.
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