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NEDD8 Antibody (H-2): sc-373741
- NEDD8 Antibody (H-2) is a mouse monoclonal IgG1 κ NEDD8 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-81 representing full length NEDD8 of human origin
- NEDD8 Antibody (H-2) is recommended for detection of NEDD8 of mouse, rat and human origin by WB, IP, IF and ELISA; may cross-react with ubiquitin and other ubiquitin-like proteins
- Anti-NEDD8 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NEDD8 (H-2): sc-373741.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NEDD8 Antibody (H-2) for WB applications. These reagents are now offered in bundles with NEDD8 Antibody (H-2) (see ordering information below).
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NEDD8 Antibody (H-2) is an IgG1 κ mouse monoclonal NEDD8 antibody (also designated NEDD8 antibody) that detects the NEDD8 protein of mouse, rat and human origin by WB, IP, IF and ELISA. NEDD8 Antibody (H-2) is available as both the non-conjugated anti-NEDD8 antibody form, as well as multiple conjugated forms of anti-NEDD8 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. NEDD8 is a ubiquitin (Ub)-like molecule that shares 80% homology with ubiquitin, a protein known to modify and target various proteins for proteolytic degradation. NEDD8 and the corresponding yeast homolog Rub1 are activated by the E1 ubiquitin activating enzyme UBA2, which forms isopeptide linkages between thio esters. Similar to the Ub-mediated proteolytic pathway, NEDD8 is covalently coupled to an E3 Ub ligase by the E2 conjugating enzyme, Ubc12. NEDD8 preferentially associates with the CUL-2 protein in the E3 ligase complex CBCVHL, which consists of cullin proteins associating with elongin B/C and the tumor suppressor F-box protein, von Hippel Lindau (VHL). NEDD8 is predominantly localized to the nucleus and is highly expressed in adult heart and skeletal muscle. In vitro studies indicate that NEDD8 tetramers are also able to bind to the 26S Proteasome, and that they assemble into complexes with conserved Ub-like moieties, suggesting that NEDD8 may regulate proteolysis of intracellular proteins similar to other Ub-mediated pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Identification of NEDD8-conjugation site in human cullin-2. | Wada, H., et al. 1999. Biochem Biophys Res Commun. 257: 100-5. PMID: 10092517
- Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. | Gong, L. and Yeh, ET. 1999. J Biol Chem. 274: 12036-42. PMID: 10207026
- Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 is linked to von Hippel-Lindau tumor suppressor function. | Liakopoulos, D., et al. 1999. Proc Natl Acad Sci U S A. 96: 5510-5. PMID: 10318914
- Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein. | Kamitani, T., et al. 1997. J Biol Chem. 272: 28557-62. PMID: 9353319
- A new NEDD8-ligating system for cullin-4A. | Osaka, F., et al. 1998. Genes Dev. 12: 2263-8. PMID: 9694792
- The ligation systems for ubiquitin and ubiquitin-like proteins. | Tanaka, K., et al. 1998. Mol Cells. 8: 503-12. PMID: 9856335
- Crystal structure of the human ubiquitin-like protein NEDD8 and interactions with ubiquitin pathway enzymes. | Whitby, FG., et al. 1998. J Biol Chem. 273: 34983-91. PMID: 9857030