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MYL2 Antibody (7C9): sc-517244
- MYL2 Antibody (7C9) is a mouse monoclonal IgG1 κ MYL2 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 2-166 of MYL2 of human origin
- MYL2 Antibody (7C9) is recommended for detection of MYL2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-MYL2 Antibody (7C9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MYL2 (7C9): sc-517244.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MYL2 Antibody (7C9). This work is in progress.
MYL2 Antibody (7C9) is an IgG1 κ mouse monoclonal MYL2 antibody (also designated MYL2 antibody) that detects the MYL2 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. MYL2 Antibody (7C9) is available as both the non-conjugated anti-MYL2 antibody form, as well as multiple conjugated forms of anti-MYL2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Encoded by the MYL2 gene, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, also designated MLC-2 or MLC2v, is part of a hexameric complex of two heavy chains and four light chains predominantly expressed in adult cardiac ventricle muscle. Myosin regulatory light chain 2 binds calcium and has been shown to be a useful molecular marker for cardiac chamber specification. The co-expression of myosin regulatory light chain 7 (MYL7) and myosin regulatory light chain 2 in the outflow tract and atrioventricular canal, together with the single expression in the atrial (MYL7) and ventricular (MYL2) myocardium, permits the delineation of their boundaries. At the amino acid level there is 96% homology between the human and mouse myosin regulatory light chain sequences. Mutations in MYL2 are correlated with mid-left ventricular chamber type hypertrophic cardiomyopathy (MVC2) as well as familial hypertrophic cardiomyopathy type 10 (CMH10).
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- The murine atrial myosin light chain-2 gene: a member of an evolutionarily conserved family of contractile proteins. | Doevendans, PA., et al. 2000. Cytogenet Cell Genet. 90: 248-52. PMID: 11124527
- An extra human chromosome 21 reduces mlc-2a expression in chimeric mice and Down syndrome. | Nishigaki, R., et al. 2002. Biochem Biophys Res Commun. 295: 112-8. PMID: 12083776
- Chamber specification of atrial myosin light chain-2 expression precedes septation during murine cardiogenesis. | Kubalak, SW., et al. 1994. J Biol Chem. 269: 16961-70. PMID: 8207020
- Downregulation of atrial markers during cardiac chamber morphogenesis is irreversible in murine embryos. | Gruber, PJ., et al. 1998. Development. 125: 4427-38. PMID: 9778502
- Myosin light chain 2a and 2v identifies the embryonic outflow tract myocardium in the developing rodent heart. | Franco, D., et al. 1999. Anat Rec. 254: 135-46. PMID: 9892427