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HLA class I Antibody (HP-1F7): sc-69892
- HLA class I Antibody (HP-1F7) is a mouse monoclonal IgG1 κ HLA class I antibody, cited in 7 publications, provided at 200 µg/ml
- raised against NK cells of human origin
- HLA class I Antibody (HP-1F7) is recommended for detection of all classical HLA class Ia as well as HLA-B (MHC class Ib) of human origin by WB, IP and FCM
- Anti-HLA class I Antibody (HP-1F7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also provided azide-free for functional assays for antagonizing the interaction of NK inhibitory receptors with class I molecules, sc-69892 L, 200 µg/0.1 ml
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA class I (HP-1F7): sc-69892.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HLA class I Antibody (HP-1F7) for WB applications. These reagents are now offered in bundles with HLA class I Antibody (HP-1F7) (see ordering information below).
HLA class I Antibody (HP-1F7) is an IgG1 κ mouse monoclonal HLA class I antibody (also designated HLA major histocompatibility complex, class I antibody, major histocompatibility complex (MHC) class 1 antibody, chromosome 6p21 gene complex class 1 antibody, or 45 kDa transmembrane class I human leukocyte antigen antibody) that detects the HLA class I protein of human origin by WB, IP and FCM. HLA class I Antibody (HP-1F7) is available as both the non-conjugated anti-HLA class I antibody form, as well as multiple conjugated forms of anti-HLA class I antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The major histocompatibility complex (MHC) is a high genomic density gene family that plays an important role in the immune system, autoimmunity and reproductive success. Human MHC genes are referred to as human leukocyte antigen (HLA) genes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Polymorphism and evolution of HLA class I and II genes and molecules. | Little, AM. and Parham, P. 1999. Rev Immunogenet. 1: 105-23. PMID: 11256568
- Accessory proteins that control the assembly of MHC molecules with peptides. | Van Kaer, L. 2001. Immunol Res. 23: 205-14. PMID: 11444385
- The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. | Tran, TM., et al. 2001. Immunogenetics. 53: 440-6. PMID: 11685454
- The major histocompatibility complex of the rat (Rattus norvegicus). | Gυnther, E. and Walter, L. 2001. Immunogenetics. 53: 520-42. PMID: 11685465
- Molecular genetics of the HLA complex. | Fischer, GF. and Mayr, WR. 2001. Wien Klin Wochenschr. 113: 814-24. PMID: 11732117