Androgen Receptor Antibody (441): sc-7305

Androgen Receptor Antibody (441) is an IgG1 κ mouse monoclonal Androgen Receptor antibody (also designated AR antibody) that detects the AR protein of mouse, rat and human origin by WB, IP, IF and IHC(P). AR Antibody (441) is available as both the non-conjugated anti-AR antibody form, as well as multiple conjugated forms of anti-AR antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and male reproductive physiology. The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. ARs bind the two biologically active androgens, testosterone (T) and dihydrotestosterone (DHT), with high and nearly identical affinities; however, the rates of association and dissociation of T are about three times more rapid than those of DHT. This difference has resulted in speculation as to whether these differences in binding kinetics could account for the different physiological effects of T and DHT. A striking feature of AR is its rapid degradation in the absence of ligand. It is now well established that androgen binding results in an at least six-fold increase in androgen stability and that ligand-induced stabilization of AR is highly androgen- specific.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Androgen receptor level controlled by a suppressor complex lost in an androgen-independent prostate cancer cell line.  |  Wang, LG., et al. 2004. Oncogene. 23: 5175-84. PMID: 15156193
2. Androgen receptor phosphorylation, turnover, nuclear transport, and transcriptional activation. Specificity for steroids and antihormones.  |  Kemppainen, JA., et al. 1992. J Biol Chem. 267: 968-74. PMID: 1730684
3. Stabilizing androgen receptor in mitosis inhibits prostate cancer proliferation.  |  Vander Griend, DJ., et al. 2007. Cell Cycle. 6: 647-51. PMID: 17387277
4. Binding properties of androgen receptors. Evidence for identical receptors in rat testis, epididymis, and prostate.  |  Wilson, EM. and French, FS. 1976. J Biol Chem. 251: 5620-9. PMID: 184085
5. Testosterone at high concentrations interacts with the human androgen receptor similarly to dihydrotestosterone.  |  Grino, PB., et al. 1990. Endocrinology. 126: 1165-72. PMID: 2298157
6. Familial incomplete male pseudohermaphroditism, type 2. Decreased dihydrotestosterone formation in pseudovaginal perineoscrotal hypospadias.  |  Walsh, PC., et al. 1974. N Engl J Med. 291: 944-9. PMID: 4413434
7. Steroid 5alpha-reductase deficiency in man: an inherited form of male pseudohermaphroditism.  |  Imperato-McGinley, J., et al. 1974. Science. 186: 1213-5. PMID: 4432067
8. Specificity of ligand-dependent androgen receptor stabilization: receptor domain interactions influence ligand dissociation and receptor stability.  |  Zhou, ZX., et al. 1995. Mol Endocrinol. 9: 208-18. PMID: 7776971
9. The androgen receptor: an overview.  |  Zhou, ZX., et al. 1994. Recent Prog Horm Res. 49: 249-74. PMID: 8146426

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Androgen Receptor Antibody (441) has 609 citations in a variety of scientific publications.

Primary antibodies like Anti-Androgen Receptor Antibody (441) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Androgen Receptor Antibody (441) Specifications

Alternate Product Name

Androgen Receptor

Product Clone Name

441

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

299-315

Epitope Target

AR

Epitope Species

human

Product Reactivity/Detection

AR

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P)

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

609 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine, X: TransCruz Reagent

The preferred Androgen Receptor antibody is Androgen Receptor Antibody (E-6): sc-377546.