Anticorps Lamin A/C (636): sc-7292

Lamin A/C Antibody (636) is an IgG2b κ mouse monoclonal Lamin A/C antibody that detects Lamin A and Lamin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Lamin A/C Antibody (636) is available as both conjugated and non-conjugated anti-Lamin A/C antibody forms. A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, is comprised of ICE, CPP32, ICH-1/Nedd-2, Tx, Mch2, Mch3 (ICE-LAP3 or CMH-1), Mch4 and ICE-LAP6. Ced-3/ICE family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family. Lamin C is a splice variant of Lamin A, differing only at the carboxy-terminus. Lamins A and C are identical for the first 566 amino acids, with Lamin C differing only in six unique carboxy-terminal amino acids.

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References:

1. Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.  |  McKeon, FD., et al. Nature. 319: 463-8. PMID: 3453101
2. cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.  |  Fisher, DZ., et al. 1986. Proc Natl Acad Sci U S A. 83: 6450-4. PMID: 3462705
3. The dynamic properties and possible functions of nuclear lamins.  |  Moir, RD., et al. 1995. Int Rev Cytol. 162B: 141-82. PMID: 8557486
4. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
5. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
6. Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis.  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
7. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
8. Lamin proteolysis facilitates nuclear events during apoptosis.  |  Rao, L., et al. 1996. J Cell Biol. 135: 1441-55. PMID: 8978814

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Lamin A/C Antibody (636) has 456 citations in a variety of scientific publications.

Primary antibodies like Anti-Lamin A/C Antibody (636) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.