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UBR5 Polyclonal antibody

UBR5 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22782-1-AP

Synonyms

DD5, EDD, EDD1, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBR5 Polyclonal antibody (22782-1-AP)

    IHC staining of mouse testis using 22782-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBR5 Polyclonal antibody (22782-1-AP)

    IHC staining of mouse testis using 22782-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using UBR5 Polyclonal antibody (22782-1-AP)

    IHC staining of human kidney using 22782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using UBR5 Polyclonal antibody (22782-1-AP)

    IHC staining of human kidney using 22782-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using UBR5 Polyclonal antibody (22782-1-AP)

    IHC staining of human testis using 22782-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using UBR5 Polyclonal antibody (22782-1-AP)

    IHC staining of human testis using 22782-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22782-1-AP (UBR5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using UBR5 Polyclonal antibody (22782-1-AP)

    IF Staining of MCF-7 using 22782-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 22782-1-AP (UBR5 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse testis tissue, human testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22782-1-AP is UBR5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBR5 fusion protein Ag18753
Full Name ubiquitin protein ligase E3 component n-recognin 5
Calculated Molecular Weight 2799 aa, 309 kDa
Observed Molecular Weight 309 kDa
GenBank Accession NumberBC137234
Gene Symbol UBR5
Gene ID (NCBI) 51366
RRIDAB_2879166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for UBR5 antibody 22782-1-APDownload protocol
IF protocol for UBR5 antibody 22782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Gastroenterol Hepatol

C2orf40 inhibits hepatocellular carcinoma through interaction with UBR5.

Authors - Yue Wu
View Article
humanWB,IF

J Virol

UBR5 Acts as an Antiviral Host Factor against MERS-CoV via Promoting Ubiquitination and Degradation of ORF4b

Authors - Yuzheng Zhou
View Article
human,mouseWB,IHC,IF,CoIP

Sci China Life Sci

SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1

Authors - Hao Wang
View Article