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TSPAN2 Polyclonal antibody

TSPAN2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 20463-1-AP

Synonyms

6330415F13Rik, NET3, Tetraspan NET 3, tetraspanin 2, TSN2, TSPAN 2, TSPAN2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSPAN2 Polyclonal antibody (20463-1-AP)

    WB analysis using 20463-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20463-1-AP (TSPAN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using TSPAN2 Polyclonal antibody (20463-1-AP)

    WB analysis of HL-60 using 20463-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 20463-1-AP (TSPAN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using TSPAN2 Polyclonal antibody (20463-1-AP)

    IP experiment of HL-60 using 20463-1-AP

    IP result of anti-TSPAN2 (IP:20463-1-AP, 4ug; Detection:20463-1-AP 1:500) with HL-60 cells lysate 960ug.

Tested Applications

Positive WB detected inHL-60 cells, K-562 cells
Positive IP detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20463-1-AP targets TSPAN2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPAN2 fusion protein Ag14299
Full Name tetraspanin 2
Calculated Molecular Weight 221 aa, 24 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC021675
Gene Symbol TSPAN2
Gene ID (NCBI) 10100
RRIDAB_2878691
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN2 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. It has been reported that TSPAN2 is involved in cell invasion and motility during lung cancer progression (PMID: 24726368). TSPAN2 enhances cell motility and invasiveness by assisting CD44 in scavenging intracellular reactive oxygen species.

Protocols

Product Specific Protocols
WB protocol for TSPAN2 antibody 20463-1-APDownload protocol
IP protocol for TSPAN2 antibody 20463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Elife

Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.

Authors - Vasiliki-Ilya Gargareta
View Article
humanIF

FASEB J

Selective expression of TSPAN2 in vascular smooth muscle is independently regulated by TGF-β1/SMAD and myocardin/serum response factor.

Authors - Jinjing Zhao
View Article