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PTPIP51 Monoclonal antibody

PTPIP51 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G11E2

Cat no : 68582-1-Ig

Synonyms

Cerebral protein 10, FAM82A2, FAM82C, FLJ10579, hRMD 3, Protein FAM82A2, Protein FAM82C, PTPIP51, RMD 3, RMD3, TCPTP interacting protein 51, UNQ3122/PRO10274

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPIP51 Monoclonal antibody (68582-1-Ig)

    WB analysis using 68582-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68582-1-Ig (PTPIP51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, HuH-7 cells, HT-29 cells, HEK-293 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68582-1-Ig targets PTPIP51 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTPIP51 fusion protein Ag15135
Full Name family with sequence similarity 82, member A2
Calculated Molecular Weight 470 aa, 52 kDa
Observed Molecular Weight52-60 kDa
GenBank Accession NumberBC008970
Gene Symbol FAM82A2
Gene ID (NCBI) 55177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618).The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).

Protocols

Product Specific Protocols
WB protocol for PTPIP51 antibody 68582-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols