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MTFR1 Polyclonal antibody

MTFR1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17778-1-AP

Synonyms

CHPPR, FAM54A2, KIAA0009, MTFR1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTFR1 Polyclonal antibody (17778-1-AP)

    WB analysis using 17778-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17778-1-AP (MTFR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using MTFR1 Polyclonal antibody (17778-1-AP)

    WB analysis of A549 using 17778-1-AP

    WB result of MTFR1 antibody (17778-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTFR1 transfected A549 cells.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using MTFR1 Polyclonal antibody (17778-1-AP)

    IP experiment of mouse testis using 17778-1-AP

    IP result of anti-MTFR1 (IP:17778-1-AP, 4ug; Detection:17778-1-AP 1:800) with mouse testis tissue lysate 4800ug.

Tested Applications

Positive WB detected inA549 cells, HCT 116 cells, HepG2 cells, SW480 cells
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17778-1-AP targets MTFR1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTFR1 fusion protein Ag10857
Full Name mitochondrial fission regulator 1
Calculated Molecular Weight 333 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC036116
Gene Symbol MTFR1
Gene ID (NCBI) 9650
RRIDAB_2282301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTFR1, also known as CHPPR, is a mitochondria protein. It may play a role in mitochondrial aerobic respiration. It may also regulate mitochondrial organization and fission.

Protocols

Product Specific Protocols
WB protocol for MTFR1 antibody 17778-1-APDownload protocol
IP protocol for MTFR1 antibody 17778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols