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FCHSD1 Polyclonal antibody

FCHSD1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23362-1-AP

Synonyms

FCH and double SH3 domains 1, FCHSD1, NWK2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FCHSD1 Polyclonal antibody (23362-1-AP)

    WB analysis using 23362-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FCHSD1 Polyclonal antibody (23362-1-AP)

    WB analysis of Jurkat using 23362-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FCHSD1 Polyclonal antibody (23362-1-AP)

    WB analysis of HeLa using 23362-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FCHSD1 Polyclonal antibody (23362-1-AP)

    WB analysis of K-562 using 23362-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FCHSD1 Polyclonal antibody (23362-1-AP)

    WB analysis of HEK-293 using 23362-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FCHSD1 Polyclonal antibody (23362-1-AP)

    WB analysis of K-562 using 23362-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FCHSD1 Polyclonal antibody (23362-1-AP)

    WB analysis of HEK-293 using 23362-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FCHSD1 Polyclonal antibody (23362-1-AP)

    WB analysis of Jurkat using 23362-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FCHSD1 Polyclonal antibody (23362-1-AP)

    IHC staining of human breast cancer using 23362-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23362-1-AP (FCHSD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FCHSD1 Polyclonal antibody (23362-1-AP)

    IHC staining of human breast cancer using 23362-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23362-1-AP (FCHSD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using FCHSD1 Polyclonal antibody (23362-1-AP)

    IF Staining of mouse kidney using 23362-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using FCHSD1 antibody (23362-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

23362-1-AP targets FCHSD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FCHSD1 fusion protein Ag19963
Full Name FCH and double SH3 domains 1
Calculated Molecular Weight 690 aa, 77 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC047016
Gene Symbol FCHSD1
Gene ID (NCBI) 89848
RRIDAB_2879265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FCHSD1 (FCH and double SH3 domains 1), also known as NWK2, is a member of the F-BAR family, which is involved in endocytosis and vesicle trafficking. FCHSD1 is expressed in mouse cochlear sensory hair cells and plays an important role in regulating the assembly or maintenance of F-actin in hair cell stereocilia and cuticular plate (PMID: 23437151). FCHSD1 also plays a role in stress response sensing and oxidative stress integration and is involved in the pathogenesis of emphysema (PMID: 34168078). FCHSD1 has three isoforms with molecular weights of 77 kDa, 46 kDa, and 40 kDa.

Protocols

Product Specific Protocols
WB protocol for FCHSD1 antibody 23362-1-APDownload protocol
IHC protocol for FCHSD1 antibody 23362-1-APDownload protocol
IF protocol for FCHSD1 antibody 23362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.

Authors - Takahiro Kawasaki
View Article