Epinephrine Stimulates Mycobacterium tuberculosis Growth and Biofilm Formation
Abstract
:1. Introduction
2. Results
2.1. Epinephrine Stimulates M. tuberculosis Growth In Vitro
2.2. MprB Is Involved in the Regulation of M. tuberculosis Growth In Vitro by Epi
2.3. Epi Stimulates M. tuberculosis Biofilm Formation
2.4. Epi Enhances M. tuberculosis Antibiotic Resistivity and Stress Tolerance
3. Discussion
4. Materials and Methods
4.1. Bacteria Strains, Growth Conditions, and Reagents
4.2. Colony-Forming Unit (CFU) Assay
4.3. Construction of the mprB Overexpressing Strain of M. tuberculosis H37Ra and M. smegmatis mc2 155
4.4. Construction of the mprB Knock down Strain of M. tuberculosis H37Ra
4.5. Molecular Docking
4.6. Crystal Violet (CV) Assay of Biofilm
4.7. Scanning Electron Microscopy (SEM)
4.8. Confocal Laser Scanning Microscopy (CLSM)
4.9. Metabolite Extraction
4.10. Metabolism Data Analysis and Visualization
4.11. Drug Susceptibility Analysis
4.12. In Vitro Stress Susceptibility Assay
4.13. Other Insilco Analysis
4.14. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Lei, Y.; Rahman, K.; Cao, X.; Yang, B.; Zhou, W.; Reheman, A.; Cai, L.; Wang, Y.; Tyagi, R.; Wang, Z.; et al. Epinephrine Stimulates Mycobacterium tuberculosis Growth and Biofilm Formation. Int. J. Mol. Sci. 2023, 24, 17370. https://doi.org/10.3390/ijms242417370
Lei Y, Rahman K, Cao X, Yang B, Zhou W, Reheman A, Cai L, Wang Y, Tyagi R, Wang Z, et al. Epinephrine Stimulates Mycobacterium tuberculosis Growth and Biofilm Formation. International Journal of Molecular Sciences. 2023; 24(24):17370. https://doi.org/10.3390/ijms242417370
Chicago/Turabian StyleLei, Yingying, Khaista Rahman, Xiaojian Cao, Bing Yang, Wei Zhou, Aikebaier Reheman, Luxia Cai, Yifan Wang, Rohit Tyagi, Zhe Wang, and et al. 2023. "Epinephrine Stimulates Mycobacterium tuberculosis Growth and Biofilm Formation" International Journal of Molecular Sciences 24, no. 24: 17370. https://doi.org/10.3390/ijms242417370