Dysregulation of the Nitric Oxide/Dimethylarginine Pathway in Hypoxic Pulmonary Vasoconstriction—Molecular Mechanisms and Clinical Significance
Juliane Hannemann1,2† 
Rainer Böger1,2*†- 1Institute of Clinical Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
- 2Institute DECIPHER, German-Chilean Institute for Research on Pulmonary Hypoxia and its Health Sequelae, Hamburg, Germany
The pulmonary circulation responds to hypoxia with vasoconstriction, a mechanism that helps to adapt to short-lived hypoxic episodes. When sustained, hypoxic pulmonary vasoconstriction (HPV) may become deleterious, causing right ventricular hypertrophy and failure, and contributing to morbidity and mortality in the late stages of several chronic pulmonary diseases. Nitric oxide (NO) is an important endothelial vasodilator. Its release is regulated, amongst other mechanisms, by the presence of endogenous inhibitors like asymmetric dimethylarginine (ADMA). Evidence has accumulated in recent years that elevated ADMA may be implicated in the pathogenesis of HPV and in its clinical sequelae, like pulmonary arterial hypertension (PAH). PAH is one phenotypic trait in experimental models with disrupted ADMA metabolism. In high altitude, elevation of ADMA occurs during long-term exposure to chronic or chronic intermittent hypobaric hypoxia; ADMA is significantly associated with high altitude pulmonary hypertension. High ADMA concentration was also reported in patients with chronic obstructive lung disease, obstructive sleep apnoea syndrome, and overlap syndrome, suggesting a pathophysiological role for ADMA-mediated impairment of endothelium-dependent, NO-mediated pulmonary vasodilation in these clinically relevant conditions. Improved understanding of the molecular (dys-)regulation of pathways controlling ADMA concentration may help to dissect the pathophysiology and find novel therapeutic options for these diseases.
Introduction
Hypoxia is a deadly threat to every cell and to the organism as a whole. It is therefore not surprising that complex molecular mechanisms have evolved that help the cell to maintain its integrity during short-lived periods of hypoxia, as well as physiological mechanisms that help the organism to adapt to conditions of low oxygen supply.
In most organs, the response to a mismatch between oxygen demand and supply is an increase in blood flow. This has been demonstrated for the coronary, cerebral, renal, and other vascular beds (1–3). Hypoxia in the systemic circulation may result from local vascular occlusion (either by vasospasm or thromboembolism), low oxygen delivery with the blood stream (either because of anemia or reduced arterial hemoglobin oxygen content), or reduced perfusion volume (e.g., in chronic heart failure). In each case, compensatory mechanisms aiming at increasing local blood flow are activated to minimize ischemic tissue damage. Recurrent brief periods of ischemia in the systemic circulation activate mechanisms leading to improved protection of tissues from ischemic cell death. This interesting phenomenon called ischemic pre-conditioning has been extensively investigated and reviewed (4–6); further detailed description is beyond the scope of this review.
By contrast, the vast majority of tissue oxygen tension in the lung results from oxygen diffusing from the alveoli rather than being delivered with the blood stream of the bronchial arteries. Hypoxia in the lung is therefore most frequently a result of blocked airflow through the bronchial tree into the alveoli. In the lung, the vascular system responds to hypoxia with vasoconstriction rather than vasodilation. This obvious difference between hypoxic systemic vasodilation and hypoxic pulmonary vasoconstriction has aroused intense research interest for many decades ever since it was first described in the early 20th century (7, 8). However, its molecular mechanisms have remained elusive to this date.
Nitric oxide (NO) is a critically important mediator of vasodilation under a variety of physiological and pathophysiological conditions. The generation of NO, which occurs mainly in the vascular endothelium, is regulated (a) by transcriptional and posttranscriptional mechanisms affecting the NO-producing enzyme, endothelial nitric oxide synthase (eNOS), (b) by factors regulating the enzymatic activity of eNOS, and (c) by reactive oxygen species that rapidly react—and thereby inactivate—NO once released from the endothelium. The enzymatic activity of eNOS is also regulated by the presence of methylarginines (9). Asymmetric dimethylarginine (ADMA) is a competitive inhibitor of eNOS; elevated ADMA concentration has been shown to lead to impaired NO generation and endothelial dysfunction which is reversible by L-arginine (10). Individuals with elevated circulating ADMA concentration are at increased risk of cardiovascular events and mortality (11, 12). ADMA levels are regulated through its biosynthesis, which occurs during arginine methylation of proteins by protein arginine N-methyltransferases (PRMTs) (13, 14), and through its metabolism, which is facilitated by dimethylarginine dimethylaminohydrolases (DDAH) 1 and 2 (15, 16). An alternative metabolic pathway is mediated by alanine glyoxylate aminotransferase-2 (AGXT-2) (17, 18). Dysregulation of the activity or expression of enzymes regulating ADMA concentration may thus contribute to impaired NO generation, endothelial dysfunction, vasospasm, and elevated vascular resistance, both in the systemic and pulmonary circulation (19). Figure 1 depicts the enzymatic pathways involved in the biosynthesis and degradation of ADMA.
Figure 1. Schematic representation of pathways of dimethylarginine biosynthesis and metabolism. Dimethylarginines are formed during (di-)methylation of protein-bound L-arginine residues by a family of protein arginine N-methyltransferases (PRMTs). Free ADMA and SDMA are released during physiological hydrolytic protein turnover. Asymmetric dimethylarginine (ADMA) inhibits nitric oxide (NO) synthesis from L-arginine, whilst symmetric dimethylarginine (SDMA) does not directly interfere with NO synthase activity. ADMA is metabolically degraded to L-citrulline and dimethylamine by either of two isoforms of dimethylarginine dimethylaminohydrolase (DDAH). Both ADMA and SDMA can be cleaved by alanine glyoxylate aminotransferase-2 (AGXT2); this enzyme is the major pathway of SDMA clearance. Minor amounts of both ADMA and SDMA can also be excreted into the urine.
This review aims to summarize our current understanding of the molecular mechanisms and clinical significance of hypoxic pulmonary vasoconstriction, and addresses the possible role of dysregulation of the L-arginine - dimethylarginine - NO pathway in this condition, based on recent experimental and clinical studies.
The Physiology of Hypoxic Pulmonary Vasoconstriction
Obviously, the lung's physiological function is to deliver fully oxygenated blood into the systemic circulation. Any regional reduction in lung ventilation—as it may occur by blocked airflow through the bronchial tree—threatens to result in suboptimal oxygenation of the blood delivered from the lung into the systemic circulation. Therefore, pulmonary vasoconstriction in a region of hypoventilation is a mechanism to redirect blood flow to better ventilated areas of the lungs, ensuring optimal oxygen supply to all tissues (Figures 2A,B).
Figure 2. Schematic representation of the pulmonary circulation in normoxia (A) and when one bronchus is obstructed and the respective alveoli are hypoventilated (B). During normoxia in the healthy state, deoxygenated blood from the pulmonary artery flows through the capillary bed surrounding the alveoli, where it takes up oxygen and, fully oxygenated, returns through the pulmonary vein to the left atrium of the heart. Local hypoventilation of an area of the lungs causes vasoconstriction of the pulmonary arteries in the same area; thus, less blood flows through the hypoventilated area and relatively more through other, better ventilated areas, resulting in a minimal reduction of the oxygenation status of the blood returning into the systemic circulation through the pulmonary vein (Euler-Liljestrand mechanism). (C) In global hypoxia, hypoxic pulmonary vasoconstriction occurs throughout the lung. This obviously does not improve the oxygenation status of the blood, but it causes a major increase in total pulmonary vascular resistance. When this situation is maintained for longer time periods, pulmonary hypertension may occur, resulting in right ventricular hypertrophy and failure.
It was the seminal work of Euler and Liljestrand in pulmonary arteries of the cat who first linked pulmonary vasoconstriction to the maintenance of full oxygenation of the blood (20). They concluded that “[…] oxygen want and carbon dioxide accumulation have exactly the reverse local effects on the vessels of the systemic and pulmonary circulations, respectively […]. They cause a dilatation of the vessels of the working organs which need a greater blood supply than during rest, but they call forth a contraction of the lung vessels, thereby increasing the blood flow to better aerated lung areas, which leads to improved conditions for the utilization of the alveolar air.” [quotation from Euler and Liljestrand (20)]. Ever since, this phenomenon has been known as the Euler-Liljestrand-mechanism. In 1955, Blakemore and co-workers demonstrated the existence of this same mechanism in humans. In healthy human subjects, they ventilated one lobe of the lung with physiologically oxygenated air and the other lobe with only 5% oxygen. They observed a redistribution of pulmonary blood flow toward the better oxygenated lobe of the lung (21).
Clinical Relevance of Hypoxic Pulmonary Vasoconstriction
Physiologically, hypoxic pulmonary vasoconstriction (HPV) is a mechanism maintaining ventilation-perfusion matching and ensuring optimal oxygenation of blood. Table 1 summarizes clinical conditions in which HPV plays a pathophysiological role. Redirection of blood flow within the lung may become relevant to limit the detrimental influence of a pathogen in pneumonia, where HPV helps to divert blood flow away from regions of inflammatory infiltration toward healthy lung areas (34). However, the vasoconstrictor mechanism may become diminished in chronic pulmonary infection, and patients may experience hypoxemia in severe pneumonia (35). In bronchial asthma, bronchoconstriction may be spatially distributed in different parts of the lung; again, HPV helps to maintain ventilation-perfusion matching and minimize hypoxemia (31, 41).
Table 1. Clinical conditions associated with pulmonary hypoxia.
HPV is also a mechanism keeping blood flow away from the still collapsed lungs in the fetus (32). However, after birth, focal atelectasis and pneumonia may occur. HPV helps to optimize systemic arterial oxygen pressure without altering pulmonary artery pressure (42).
Chronification of Hypoxic Pulmonary Vasoconstriction
When ventilation obstacles become chronic like in chronic obstructive lung disease, hypoxic pulmonary vasoconstriction often persists. Acting together with inflammatory and adaptative processes that stipulate remodeling of and fibrosis in the pulmonary vasculature (43), this may lead to persistently elevated pulmonary vascular resistance and structural changes in the pulmonary vascular walls during the progression of the disease and be a cause of pulmonary hypertension, right ventricular hypertrophy, and—finally—failure (33, 44). In chronic thromboembolic pulmonary hypertension (CTEPH, also classified as group IV of the WHO classification of pulmonary hypertension), thrombotic occlusion of a segmental pulmonary artery per se increases total pulmonary vascular resistance; However, secondary mechanisms may be triggered in the non-occluded pulmonary vessels that cause vascular remodeling and lead to a progressive further increase in total pulmonary vascular resistance (45, 46).
Global Pulmonary Hypoxia
Another cause of pathological consequences of HPV is exposure to global pulmonary hypoxia (Figure 2C). This may occur at high altitude, when hypoxia results from the low ambient pressure (hypobaric hypoxia). Acute exposure of non-acclimatized individuals to high altitude, as it can be seen in unexperienced climbers and tourists engaging in mountaineering activities, can lead to high-altitude pulmonary oedema (22). This oedema results from global but heterogeneous HPV with increased pulmonary perfusion pressure acting on the capillary bed, which becomes leaky to protein (47). High altitude pulmonary oedema can be resolved by returning to sea level (22). Residents of high altitude of different ethnic origins show different levels of adaptation to the consequences of chronic global pulmonary hypoxia. Indians native to the Andean highlands at 3,500–4,000 m have a high prevalence of hypoxic pulmonary hypertension (23), whilst inhabitants of the Tibetan plateau living at altitudes of ≥ 3,500 m rarely develop polycythaemia and pulmonary hypertension (48). One major factor contributing to altitude adaptation in Tibetans was reported to be accumulation of genetic polymorphisms in EGLN1, the gene encoding for HIF-2α (49–51). This is in accordance with the important role of HIF-2α in hypoxia-induced upregulation of erythropoietin expression (52).
The main desired effects of high altitude training also depend on hypoxia-inducible factor-2α (HIF2α)-mediated regulation of gene expression, e.g., transcriptional upregulation of erythropoiesis and subsequent improvement in oxygen transport capacity of the blood. However, the combined decreases in arterial oxygen saturation and cardiac output at altitude may limit aerobic exercise capacity, which can be resolved when lowering pulmonary arterial pressure, e.g., by treatment with an ET-1 antagonist (53), but not by acetazolamide treatment (54).
Diminished HPV may be a common mechanism of adaptation to life at high altitude: Cattle native to lowlands exhibit marked hypoxic pulmonary vasoconstriction when exposed to high altitude, resulting in an incidence of about 20% of pulmonary hypertension, pulmonary oedema, and right ventricular failure (55), a condition named brisket disease after the resulting oedema in the cows' necks (56). Interestingly, neonatal calves chronically exposed to high altitude progressively loose the vasodilator response of pulmonary arteries to acetylcholine, a well-characterized stimulus of endothelial NO release (57). This finding points to diminished NO-mediated pulmonary arterial vasodilation as a possible contributor to HPV. By contrast, yaks native to the high altitude of the Himalayan region exhibit diminished HPV and maintain low pulmonary arterial pressure (58). A recent study showed that yaks differ from cattle by lower circulating levels of ADMA and higher protein expression and activity of DDAH, the enzyme inactivating ADMA (59), supporting a role for modulation of the NO pathway in adaptation of the pulmonary circulation to high altitude.
A clinical condition that has been more recently defined is called chronic intermittent hypobaric hypoxia. Workers in mines of the Andean plateau at altitudes above 3,500 m, frontier officials, and other individuals may be exposed to working shifts alternating between several days at high altitude, followed by a few days of rest at sea level (60, 61). This leads to frequent cycling of affected individuals between the acute adaptation to hypoxia at high altitude and relief. In consequence, changes to the pulmonary circulation may occur that are very similar and may be as severe as in chronic hypobaric hypoxia (24, 62). The prevalence of elevated mean pulmonary arterial pressure (mPAP) with mPAP ≥ 25 mm Hg was reported to be as high as 26% and the prevalence of high altitude pulmonary hypertension [the threshold of which has been defined at mPAP ≥ 30 mm Hg (63)] was about 9% in chronic intermittent hypobaric hypoxia (24). Based on a meta-analysis of multiple large cohorts, systolic pulmonary arterial pressure (sPAP) at sea level was calculated to be (median [95% CI]) 18.4 [17.1–19.7] mm Hg, whilst sPAP at high altitude was 25.3 [24.0–26.7] mm Hg (64). As the threshold of mPAP for the definition of pulmonary arterial hypertension in lowlanders has recently been reduced to mPAP ≥ 20 mm Hg (65), an updated, evidence-based definition of pulmonary arterial hypertension at high altitude appears urgently needed (66).
Pulmonary hypertension is also one pathological consequence of chronic intermittent hypoxia in obstructive sleep apnoea syndrome (OSAS); increased pulmonary arterial pressure may occur during sleep, but also during waking hours (29). Whilst clinically relevant pulmonary hypertension is rare in pure OSAS, it may occur much more frequently in the so-called overlap syndrome, i.e., the combined occurrence of OSAS and chronic obstructive pulmonary disease (COPD) (30). Although there still remain gaps in our understanding of the pathophysiology of this relationship (67), one relevant observation helping us to understand the association of OSAS with vascular disease in both, the pulmonary and systemic circulation, is the presence of endothelial dysfunction, i.e., the inability of the vascular endothelium to generate physiological amounts of NO as required to maintain vasodilator tone (68).
Recent interest has focussed on the role of pulmonary vascular damage and endothelial dysfunction in COVID-19 pneumonia and ensuing hypoxaemia and organ failure (69, 70). We have reported that high ADMA and SDMA serum levels are superior biomarkers to predict COVID-19-associated in-hospital mortality (71), suggesting that NO deficiency may aggravate pulmonary and systemic vascular dysfunction in this disease. Accordingly, several small trials investigated the effects of inhaled NO (72, 73) or the phosphodiesterase V inhibitor sildenafil on COVID-19-associated hypoxaemia and outcome (74). However, the reported results of these studies have so far been inconclusive.
Mechanisms of Hypoxic Pulmonary Vasoconstriction
The best known transcriptional regulators of the physiological responses are the hypoxia-inducible factors (HIF). HIF-1α is activated acutely upon oxygen deficiency, whilst HIF-2α mediates the sustained responses to prolonged hypoxia (75). By this mechanism, hypoxia elicits a systemic hemodynamic response via activation of the carotid chemokine receptors and systemic humoral mechanisms. In addition, hypoxia also acts locally on the pulmonary vessels, thereby modulating the relation between pulmonary blood flow and alveolar ventilation. Although HIF-1 target genes have been shown to be involved in the pulmonary arterial response to hypoxia (76), the cellular crosstalk in the hypoxic lungs appears to be more complex, and the exact molecular and cellular nature of this local mechanism of HPV has remained elusive so far. A number of determinants can be defined, however, that are prerequisites of a locally functioning physiological mechanism:
A) There must be an oxygen sensor at the level or in the immediate adjacency of the pulmonary alveoli and pulmonary blood vessels.
B) There must be a locally functioning vasoconstrictor mechanism activated and / or vasodilator mechanism diminished by hypoxic signaling. This mechanism must be rapidly activated, reversible in nature, and evocable by mild hypoxia.
There are three major cell types in the lung, of which each may be responsible for initiating HPV: endothelial cells and vascular smooth muscle cells of the pulmonary arterioles, and alveolar epithelial cells lining the bronchioli and alveoli. The endothelial cells form the physiological barrier between the circulating blood and the adjacent vascular tissue, they are the major source of effectors influencing the vasoconstrictor and vasodilator properties of blood vessels. As such, they are predisposed to interlace between changes in tissue oxygen content and vascular tone by generating vasoactive mediators (see below). The vascular smooth muscle cell is less easily capable of sensing the blood oxygen content due to its more distant spatial localization. However, a hypothetical oxygen sensor located in the vascular smooth muscle cell itself could directly modulate the cell's contractile properties. The alveolar epithelial cells, on their turn, are the primary cells exposed to low oxygen content in the breathing air, and therefore predisposed to act as sensor cells. Thus, the complexity of this intercellular cross-talk may at least partly explain that the exact molecular mechanism of HPV has not yet been unraveled. Finally, different cell types or signaling mechanisms may be involved in mediating the early and late phases of HPV.
Oxygen Sensing
One of the most extensively studied sites of oxygen sensing is the carotid body, which regulates major neuroendocrine responses to hypoxemia. Carotid body glomus cells respond to hypoxemia by inhibition of K+ channels, leading to membrane depolarization, calcium influx via voltage-gated Ca2+ channels, and neuroendocrine secretion (77, 78). In the pulmonary circulation, the cellular and molecular identity of the oxygen sensor has remained much less clear. Experiments demonstrating that redox agents and certain inhibitors of complexes I and III of the mitochondrial electron transport chain cause vasoconstriction in the pulmonary vascular bed, but vasodilation in the fetal ductus arteriosus (79)—mimicking the differential responses to hypoxia in these two vascular beds—suggest that redox mechanisms may be involved. Thus, research to identify the pulmonary oxygen sensor has focused on NADPH oxidases and on the mitochondrial respiratory chain (78), and models aiming to explain HPV based on mitochondrial oxygen sensing have been proposed (80–82). In line with this, knockdown of NADH dehydrogenase ubiquinone iron-sulfur protein-2 (Ndufs-2) within the mitochondrial complex I significantly decreased hypoxic vasoconstriction in pulmonary artery smooth muscle cells (83). Another source of oxygen-derived radicals during hypoxia and ischemia episodes is accumulation of succinate, an intermediate metabolite in the mitochondrial citric acid cycle (84). Accumulation of succinate stimulates mitochondrial production of reactive oxygen species by reversing electron transport at mitochondrial complex I (85). Through this mechanism, succinate overload in hypoxia is known to activate HIF-1α (86). During normoxia, the HIF-1α protein is hydroxylated by prolyl hydroxylases that are absolutely dependent on the presence of oxygen. Hydroxylation enables binding of HIFs to the ubiquitin proteasome system and subsequent degradation; inhibition of this degradation pathway in hypoxia activates HIF-mediated gene transcription (75, 76).
Recent studies also suggest that pulmonary and systemic arteries share the same oxygen sensing mechanism within mitochondria, whilst differences in downstream signaling of reactive oxygen species released from hypoxic mitochondria cause site-specific vascular responses (87). As the three major cell types present in the lung have all been shown to be responsive to hypoxia (81, 88, 89), the cellular location of the oxygen sensor has remained controversial.
Signal Transduction and Effector Mechanisms: The Vascular Smooth Muscle Cell
HPV is brought about by a contractile response of the pulmonary vascular smooth muscle cells (VSMC). Smooth muscle cell contraction is highly dependent on elevated cytosolic calcium concentration; therefore, the effector mechanisms responsible for HPV likely involve modulation of VSMC calcium handling. Sarcoplasmic calcium channels, voltage-dependent potassium channels, transient receptor potential channels, and L-type calcium channels are the main regulators of cytosolic calcium (90). The coordinated response of these ion channels is influenced by protein kinases and reactive oxygen species (ROS). The Ca2+ influx directly triggers a conformational change of the myosin light chain, thereby facilitating interaction with actin filaments and contraction. Several studies have provided evidence for an involvement of ion channels in HPV: For example, inhibition of voltage-dependent potassium channels caused vasoconstriction in the isolated perfused rat lung (91). Furthermore, inhibition of L-type calcium channels diminished whereas activation of these channels enhanced the vasoconstrictor response to hypoxia (92, 93). However, the modulation of vascular tone by these channels does not differ between systemic and pulmonary arteries. Therefore, this mechanism cannot explain the heterogeneous response to hypoxia (vasoconstriction vs. vasodilation) in pulmonary and systemic arteries, respectively.
Signal Transduction and Effector Mechanisms: The Vascular Endothelial Cell
Endothelium-derived vasoactive mediators are major regulators of vascular tone in the systemic circulation. The endothelium-dependent vasoconstrictor substances include the peptide endothelin-1 (ET-1) (94), superoxide anions (95), and arachidonic acid-derived endoperoxides and/or thromboxane A2 (96). The endothelium-derived relaxing factors include NO, prostacyclin, and endothelium-derived hyperpolarizing factor (EDHF) (97). Both endothelial vasoconstrictor and vasodilator mediators are finely tuned to maintain the homeostasis of local blood flow and its adaptation to varying needs of oxygen and nutrient demand (Figure 3). Less information is available about the role of endothelium-derived mediators in the regulation of pulmonary vascular tone.
Figure 3. Schematic overview of endothelium-derived vasoconstrictor and vasodilator mediators. The endothelium produces several vasoconstrictor mediators like endothelin-1 (ET-1) and thromboxane (TX) A2 as well as vasodilator mediators like nitric oxide (NO), prostacyclin (PGI2), and endothelium-derived hyperpolarizing factor (EDHF) that diffuse to the adjacent smooth muscle cells that effect changes in vascular tone upon this stimulation. For further details see text.
ET-1 is the most potent vasoconstrictor peptide released by endothelial cells (94). Human ET-1 is synthesized as a 212-amino acid peptide (prepro-ET-1); it exerts a long-lasting vasoconstrictor effect by activating ETA receptors (98). By contrast, binding of ET-1 to ETB receptors, which are located on the endothelial cell membrane, causes vasodilation and anti-mitogenic effects through the release of NO and/or prostacyclin (PGI2) (99, 100). The lung is an important site of ET-1 production, with ET-1 mRNA being five times more abundant in the lung than in other organs (101). Lowering oxygen levels in cultured endothelial cells rapidly increases the mRNA expression of prepro-ET-1 (102). This effect persists for at least 48 h when hypoxia is maintained, and it is reversible after increasing oxygen tension to normal ambient pressure. These experimental findings are in line with in vivo observations from animal studies (103–105), and with the observation that circulating ET-1 is elevated in COPD patients with chronic hypoxia (106). However, the endothelin receptor antagonist bosentan had variable effects on HPV in animal models and clinical studies (107–109). This may be due to the fact that bosentan is a dual blocker of both ETA and ETB receptors. Hypoxia enhances the expression of ETA and ETB receptors in the lung, but there is evidence for a predominant upregulation of ETB receptors. Thus, under hypoxic conditions, the effect of bosentan in the pulmonary circulation may be dominated by blocking ETB-mediated vasodilation (110).
Arachidonic acid metabolites are released from endothelial cells upon stimulation with acetylcholine, serotonin, adenosine diphosphate (ADP), and other substances. Based on the expression of cyclooxygenase and the spectrum of prostaglandin synthases in a specific cell type, either the vasodilator metabolites prostacyclin and PGE2, or the vasoconstrictor endoperoxides and thromboxane A2 may be released. For example, stimulation of isolated aortic rings from Wistar rats with acetylcholine results in endothelium-dependent vasodilation, whereas aortic rings from spontaneously hypertensive rats (SHR) respond with vasoconstriction (111). Aortic vasoconstriction in SHR is enhanced when endothelial NO production is blocked, whilst vasodilation is unmasked when cyclooxygenase activity is blocked (112). During chronic hypoxia, mouse pulmonary arteries release less prostacyclin and more 8-iso-prostaglandin F2α [a lipid peroxide product derived from non-enzymatic oxidation of arachidonic acid by superoxide anion (113)]. Cyclooxygenase-2 is upregulated, and endothelium-dependent relaxation in normoxia is shifted to an endothelium-independent, thromboxane receptor-dependent contraction (114).
NO is the major endothelial vasodilator mediator in the systemic and in the pulmonary circulation. In most arterial beds, it is only under pathophysiological conditions when NO signaling is impaired or under experimental conditions when NO production is pharmacologically or genetically inhibited that a significant role can be determined for other endothelial mediators. During the recent years, our research has focused on the regulation of the NO pathway by endogenous, methylated analogs of L-arginine, the physiological precursor of NO (115, 116). Evidence has accumulated that dysregulation of the NO pathway by ADMA may be involved in HPV and pulmonary hypertension (117).
Signal Transduction and Effector Mechanisms: The Alveolar Epithelial Cell
Alveolar epithelial cells are the cell type most directly exposed to decreased oxygen content in the inspired air. Type II alveolar epithelial cells make up about two thirds of the alveolar epithelial surface in the normal human lung; they play an important role in surfactant production and recycling (118). Early experiments had shown that in the isolated perfused cat lung, ventilation with low oxygen gas increased, but perfusion with partially deoxygenated blood did not increase pulmonary vascular resistance, suggesting that oxygen content in the inspired air, but not hypoxemia in the pulmonary blood vessels stipulates HPV (119). More recent experiments showed differential effects of hypoxia on human alveolar epithelial cells and human pulmonary microvascular endothelial cells, respectively, with the alveolar epithelial cells displaying a more sensitive response to hypoxia (120). Others revealed that acute changes in inspired oxygen tension are sensed by large conductance calcium-activated potassium channels of human alveolar epithelial cells (121), causing membrane hyperpolarization. Beyond that, alveolar epithelial cells are capable of secreting paracrine mediators which may influence the function of adjacent endothelial and vascular smooth muscle cells; amongst such mediators, NO derived from inducible NOS in type II alveolar epithelial cells (122), interleukin-33, and the receptor for advanced glycation end products (RAGE) have been identified [for review, cf. (89)]. Thus, alveolar epithelial cells may be involved in sensing hypoxia and mediating this signal to vascular endothelial and smooth muscle cells, thereby contributing to pulmonary vascular contraction and remodeling in hypoxia (123).
Dysregulation of the Endothelial NO Pathway in the Hypoxic Pulmonary Circulation
Acute and chronic hypobaric hypoxia at high altitude result in endothelial dysfunction, a situation defined by impaired endothelium-dependent, NO-mediated vasodilation in response to brief phases of ischemia in the forearm or in response to local infusion of acetylcholine. Endothelium-dependent vasodilation is acutely impaired in lowlanders after arrival to high altitude hypoxia (124) as well as in Tibetan inhabitants of the Himalaya region, despite the good genetic adaptation of this population to chronic hypobaric hypoxia (125). Inhabitants of the Andean high altitude region also show distinct endothelial dysfunction, which is more pronounced in individuals with cardiovascular risk factors or overt cardiovascular disease than in controls (126).
The underlying mechanisms leading to dysfunction of the NO pathway have been extensively studied and are considered to be multifactorial. Changes in eNOS gene expression, reduced eNOS catalytic activity, altered L-arginine metabolism, and increased NO consumption by reaction with superoxide anion may all contribute to a lack of bioactive NO.
There is evidence of markedly decreased eNOS gene expression in the endothelium of patients with pulmonary hypertension (127). However, subsequent studies found pulmonary expression of eNOS unchanged in pulmonary hypertension (128), and some studies even reported increased expression of eNOS and/or the inducible isoform of NOS (129). Thus, NOS gene expression does not always correspond to NO production, as NOS activity may be influenced by several factors relevant to pulmonary hypoxia.
Endothelial NOS needs a variety of co-factors to function normally [reviewed in Förstermann and Sessa (130) and Moncada and Higgs (131)]. When the endothelial cell is depleted of co-factors, eNOS becomes “uncoupled,” i.e., its catalytic activity is driven toward the generation of superoxide anions (130). Specifically, oxidation of the essential eNOS co-factor tetrahydrobiopterin has been shown to cause uncoupling of eNOS activity and endothelial dysfunction.
Another cause of diminished eNOS activity may be the presence of endogenous NOS inhibitors. Table 2 summarizes experimental evidence from animal models for a link between dimethylarginine metabolism, hypoxia, and pulmonary arterial hypertension. ADMA is produced during the post-translational methylation of arginine residues within specific proteins (13, 144). When methylated proteins are cleaved, ADMA is released instead of L-arginine. ADMA competes with L-arginine for binding to the NOS catalytic site and thus competitively inhibits NOS activity. Another dimethylarginine, symmetric dimethylarginine (SDMA), is unable to directly interfere with NOS activity, but like ADMA, it may inhibit CAT-2, the cellular uptake transporter for L-arginine (145, 146). We have recently reviewed in detail the transcriptional and post-translational mechanisms of regulation of dimethylarginine metabolism (9). Dimethylation of proteins occurs as a process of posttranslational protein modification and leads to increased hydrophobicity of the respective protein moieties. This process is ubiquitously present in all tissues investigated so far, although the specific types of protein arginine N-methyltransferases (PRMT) may vary in a tissue-specific manner. Amongst highly dimethylated proteins are heterogeneous nuclear ribonucleoproteins. Histone proteins are activated by asymmetric dimethylation and repressed by symmetric dimethylation, this affects their regulatory roles in gene expression (147, 148). Myelin basic protein is a neuronal protein that is known to be highly symmetrically dimethylated (149), a fact that may explain why high SDMA concentrations can be found in cerebral ischemic stroke (150, 151). Physiological turnover of proteins releases either ADMA or SDMA, depending on the type of methylation of the degraded protein. Although several PRMT enzymes are expressed in the lungs, it is not known whether asymmetric or symmetric demethylation plays a functional role in the lungs or in the vascular system.
Table 2. Experimental models linking derangement of the ADMA/DDAH pathway with pulmonary hypoxia and pulmonary vascular dysfunction.
ADMA is mainly degraded by the enzyme dimethylarginine dimethylaminohydrolase (DDAH), which exists in two isoforms. DDAH-1 has been described as the major isoform in the kidneys and liver, whilst DDAH-2 is expressed mainly in vascular tissues (16, 152). Derangement of DDAH, either genetically induced in knockout mouse models, pharmacologically caused by DDAH-inhibitory compounds, or biochemically caused by high glucose or oxidative stress, leads to elevated ADMA that impairs NO generation by eNOS and results, amongst other effects, in elevated pulmonary arterial pressure (136).
Evidence for Dysregulation of the Dimethylarginine Pathway in Pulmonary Hypoxia and Pulmonary Arterial Hypertension
In patients with different pulmonary diseases, ADMA levels are higher than in healthy controls (Table 3). Specifically, elevated ADMA has been reported in patients with obstructive sleep apnoea syndrome (OSAS) and in those with chronic obstructive lung disease (COPD). Both conditions are associated with hypoxemia, the development of elevated pulmonary artery pressure, pulmonary arterial hypertension, and right heart failure, as well as a high risk of systemic cardiovascular disease (183, 184). Multiple small cross-sectional studies reported higher plasma or serum ADMA in COPD than healthy controls; in addition, some studies reported an inverse correlation between ADMA and FEV1 or COPD severity grade (167, 172), or significantly higher ADMA in acutely exacerbated than in stable COPD (170, 173). High ADMA was associated with intima-media thickness in the brachial artery of COPD patients (169) and inversely associated with serum NO metabolites in another study (170). Lastly, ADMA and SDMA had prognostic relevance in a prospective study with 150 patients with acutely exacerbated COPD; the highest quartiles of ADMA and SDMA were significantly associated with all-cause mortality after 6 years of follow-up (mortality rate, 54%) (170).
Table 3. Clinical conditions of pulmonary hypoxia in which derangement of the ADMA / DDAH pathway was described.
Data on plasma or serum ADMA concentrations are more controversial in OSAS. Some case-control studies reported higher ADMA concentration in OSAS (157, 159, 161), along with lower NO metabolite levels (162) or impaired endothelium-dependent vasodilation (185). However, other investigators were unable to reproduce these findings (156, 177). Interpretation of these studies is hampered by methodological flaws in some studies, by lack of healthy controls in others, and by differences and—in some studies—uncertainties about analytical methods utilized for ADMA quantification.
Additionally, elevated ADMA has been measured in several types of pulmonary arterial hypertension (179, 181, 182). A prospective study reported that elevated ADMA is associated with impaired long-term survival of patients with primary pulmonary arterial hypertension (178), a finding in line with the reported role of ADMA as a marker of long-term cardiovascular events and mortality in the general population (11, 186, 187).
We and others have studied the effects of chronic hypobaric hypoxia and chronic intermittent hypobaric hypoxia on the regulation of the NO/ADMA pathway in a number of experimental models and clinical cohorts. Rats that were exposed to chronic hypobaric hypoxia for 30 days developed right ventricular hypertrophy, diminished DDAH activity, and elevated circulating ADMA levels (141). Despite upregulated eNOS mRNA expression, the biological activity of NO was unchanged, suggesting that NOS activity was inhibited by elevated ADMA. In young, healthy humans who were exposed to high altitude (3,500 m) for the first time in an intermittent, weekly exposure regimen for 3 months developed a progressive elevation of circulating ADMA levels that significantly correlated with the elevation of haematocrit (153). In a cross-sectional study of Chilean mining workers who had been exposed to intermittent work at elevations of 4,400–4,800 m for more than 5 years, elevated ADMA levels were also significantly associated with elevated mean pulmonary artery pressure (24). Recent genetic analyses performed in our laboratory revealed significant associations of single nucleotide polymorphisms (SNPs) in the NOS III, DDAH1, AGXT2, and ARG2 genes with high altitude pulmonary hypertension (188). Specifically, individuals homozygous for the minor allele of DDAH1 SNP rs233112 had higher baseline ADMA plasma concentration but no change in the ADMA response to hypoxia (188). By contrast, homozygous carriers of the minor allele of the rs805304 SNP in the DDAH2 gene had a diminished ADMA increase during hypoxia but no difference in baseline ADMA concentration. In a parallel animal study, DDHA1 ko mice showed no difference in hypoxia-induced pulmonary arterial pressure or right ventricular morphology as compared to wild-type littermates (143). DDAH1 knockout mice, however, displayed pulmonary upregulation of DDAH2 protein during chronic hypoxia, predominantly in alveolar epithelial cells, suggesting that DDAH2 upregulation may compensate for deficient DDAH1 expression and/or activity and thereby limit the pathophysiological consequences of chronic hypoxia on pulmonary vascular NO function. To a similar point, we observed a gradual decline of SDMA in humans exposed to chronic intermittent hypoxia at altitude, which paralleled the gradual increase in ADMA as reported above (60). Homozygous carriers of AGXT2 rs37369 showed a greater reduction in plasma SDMA than carriers of the minor allele of this SNP, suggesting an upregulation of AGXT2 in hypoxia (188).
Conclusions and Future Perspectives
Dysfunctional endothelium-dependent, NO-mediated vasodilation contributes to sustained HPV. There is accumulating evidence that elevated concentrations of the endogenous NOS inhibitor, ADMA, are involved in downregulating pulmonary vascular NO production in chronic hypoxia. Whilst studies in animal models and clinical cohort studies at high altitude are useful to dissect the molecular mechanisms of this regulation, it may have important clinical impact in understanding the pathophysiology of chronic pulmonary diseases like COPD and OSAS. Current evidence suggests that downregulation of DDAH mediates hypoxic accumulation of ADMA, but data are controversial as to which isoform is involved. Further, there may be compensatory regulation of one DDAH isoform when the other one is dysfunctional as suggested by a recent study in DDAH1 ko mice, as well as upregulation of AGXT2, as suggested by recent human studies. More studies are required to clarify the mechanism of this regulation. Information on a possible dysregulation of the L-arginine – dimethylarginine – NO pathway in chronic lung diseases like COPD, OSAS, overlap syndrome, and PAH are mostly derived from small, cross-sectional studies. Small patient numbers, heterogeneous patient populations and study designs, as well as methodological shortcomings contribute to current incertitude in this field. Large, prospective biomarker studies as well as mechanistic clinical studies in acute and chronic hypoxia using state-of-the-art methods are needed to shed light on the role of this pathway in chronic hypoxic lung diseases. This may open up new avenues for better treatment of chronic hypoxia and its pulmonary and systemic hemodynamic consequences.
Author Contributions
JH and RB contributed equally to data acquisition and writing. Both authors agreed to the final version of the manuscript.
Funding
This work was funded by the German Federal Ministry of Education and Research under Grant no. 01DN17046 (DECIPHER). Work by the authors was also funded by the Georg and Jürgen Rickertsen Foundation, Hamburg, Germany, the Joachim Herz Foundation, Hamburg, Germany, and the Werner Otto Foundation, Hamburg, Germany (Grant no. 02/96).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher's Note
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Keywords: high altitude, endothelium/physiopathology, asymmetric dimethylarginine (ADMA), hypoxaemia, chronic obstructive lung disease (COPD), obstructive sleep apnea syndrome (OSAS)
Citation: Hannemann J and Böger R (2022) Dysregulation of the Nitric Oxide/Dimethylarginine Pathway in Hypoxic Pulmonary Vasoconstriction—Molecular Mechanisms and Clinical Significance. Front. Med. 9:835481. doi: 10.3389/fmed.2022.835481
Received: 14 December 2021; Accepted: 27 January 2022;
Published: 17 February 2022.
Edited by:
Olivier Sitbon, Université Paris-Saclay, FranceReviewed by:
Cheng Jun Hu, University of Colorado, United StatesDavid Wu, University of Chicago, United States
Copyright © 2022 Hannemann and Böger. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Rainer Böger, boeger@uke.de
†ORCID: Juliane Hannemann orcid.org/0000-0003-3499-4827
Rainer Böger orcid.org/0000-0003-3770-5357