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A comparative transcriptome analysis of the head of 1 and 9 days old worker honeybees (Apis mellifera)

Published online by Cambridge University Press:  13 December 2022

Javad Nazemi-Rafie Open the ORCID record for Javad Nazemi-Rafie [Opens in a new window] ,
Foad Fatehi  and
Shabnam Hasrak
Javad Nazemi-Rafie*
Affiliation:
Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Kurdistan, Iran
Foad Fatehi*
Affiliation:
Department of Agriculture, Payame Noor University, Tehran, Iran
Shabnam Hasrak
Affiliation:
Genome Center, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
*
Author for correspondence: Javad Nazemi-Rafie, Email: j.nazemi@uok.ac.ir; Foad Fatehi, Email: ffatehi@pnu.ac.ir
Author for correspondence: Javad Nazemi-Rafie, Email: j.nazemi@uok.ac.ir; Foad Fatehi, Email: ffatehi@pnu.ac.ir
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Abstract

The role of bees in the environment, economic, biodiversity and pharmaceutical industries is due to its social behavior, which is oriented from the brain and hypopharyngeal gland that is the center of royal jelly (RJ) production. Limited studies have been performed on the head gene expression profile at the RJ production stage. The aim of this study was to compare the gene expressions in 9 and 1-day-old (DO) honeybee workers in order to achieve better understanding about head gene expression pattern. After sequencing of RNAs, transcriptome and their networks were compared. The head expression profile undergoes various changes. 1662 gene transcripts had differential expressions which 1125 and 537 were up and down regulated, respectively, in 9_DO compared with 1_DO honey bees. The day 1th had more significant role in the expression of genes related to RJ production as major RJ protein 1, 2, 3, 5, 6 and 9 encoding genes, but their maximum secretion occurred at day 9th. All process related to hypopharyngeal glands activities as CYP450 gene, fatty acid synthase gene, vitamin B6 metabolism and some of genes involved in fatty acid elongation and degradation process had an upward trend from 1_DO and were age-dependent. By increasing the age, the activity of pathways related to immune system increased for keeping the health of bees against the chemical compound. The expression of aromatic amino acid genes involved in Phenylalanine, tyrosine and tryptophan biosynthesis pathway are essential for early stage of life. In 9_DO honeybees, the energy supplying, reducing stress, protein production and export pathways have a crucial role for support the body development and the social duties. It can be stated that the activity of honeybee head is focused on energy supply instead of storage, while actively trying to improve the level of cell dynamics for increasing the immunity and reducing stress. Results of current study identified key genes of certain behaviors of honeybee workers. Deeper considering of some pathways will be evaluated in future studies.

Keywords

Apis melliferagene expressionnursing behaviorRNA-seqroyal jelly
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 113 , Issue 2 , April 2023 , pp. 253 - 270
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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Footnotes

*

The first two authors have contributed equally to this work as co-first authors.

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