BD Rhapsody™ Express Single-Cell Analysis System from BD Biosciences

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ItemBD Rhapsody™ Express Single-Cell Analysis System
CompanyBD Biosciences
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Catalog NumberBD Rhapsody Express
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BD Biosciences

2350 Qume Drive

San Jose, California 95131

United States

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Website: http://www.bdbiosciences.com/

(1) Augmented interferon regulatory factor 7 axis in whole tumor cell vaccines prevents tumor recurrence by inducing interferon gamma-secreting B cellsOncoImmunologyDecember 31, 2023Nabeel Kajihara, Yoshino Tanaka, Riko Takeuchi, Takuto Kobayashi, Masafumi Tanji, Tsukasa Ataka, Shiho Nakano, Taisho Yamada, Akinori Takaoka, Yoshinori Hasegawa, et al.or 4T1S. Isolated lymph node cells were prepared as libraries from cell suspension according to the BD Rhapsody™ Express Single-Cell Analysis System Instrument User Guide. Briefly, cells were lysed, cDNA was synthesized using the BD Rhapsody™ WTA Amplification

(2) Single-cell RNA-Seq reveals intracellular microbial diversity within immune cells during SARS-CoV-2 infection and recoveryiScienceNovember 17, 2023Sunita Yadav, Priyanka Mehta, Jyoti Soni, Partha Chattopadhyay, Priti Devi, Thierry Habyarimana, Kishore Tardalkar, Meghnad Joshi, Rajesh Pandeyguide (Doc ID: 214419 Rev. 2.0). An average of 30,000 pooled cells were loaded in each cartridge on the BD Rhapsody express single cell analysis system for single cell capture, followed by cDNA synthesis (BD Rhapsody cDNA kit, catalogue no 633773), as

(3) Dissecting the Mechanisms Underlying the Cytokine Release Syndrome (CRS) Mediated by T-Cell Bispecific AntibodiesClinical Cancer ResearchNovember 1, 2023Gabrielle Leclercq-Cohen, Nathalie Steinhoff, Llucia Albertí Servera, Sina Nassiri, Sabrina Danilin, Emily Piccione, Emilio Yángüez, Tamara Hüsser, Sylvia Herter, Stephan Schmeing, et al.40,000 to 50,000 cells. Single cells were isolated using Single-Cell Capture and cDNA Synthesis with the BD Rhapsody Express Single-Cell Analysis System according to the manufacturer's recommendations (BD Biosciences). cDNA libraries were prepared using

(4) Dysregulated metal ion homeostasis underscores non-canonical function of CD8+ T cell during COVID-19Frontiers in MedicineOctober 10, 2023Kriti Khare, Partha Chattopadhyay, Priti Devi, Priyanka Mehta, Aakarshan Raina, Chinky Shiu Chen Liu, Kishore Tardalkar, Meghnad G. Joshi, Rajesh Pandey2 lakh cells per sample were collected and labeled. For single cell capture, every cartridge on the BD Rhapsody express single-cell analysis system was loaded with 30,000 pooled cells, and cDNA synthesis was performed, as per the manufacturer’s instructions

(5) Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse ModelInternational Journal of Molecular SciencesSeptember 10, 2023Eun-Ji Lee, Jang-Gi Choi, Jung Ho Han, Yong-Wan Kim, Junmo Lim, Hwan-Suck ChungBD Rhapsody single-cell RNA-seq. 4.5. Single-Cell RNA Sequencing According to the protocol described in the BD Rhapsody Express Single-Cell Analysis System User Guide (BD Biosciences, San Jose, CA, USA), the single-cell suspensions (approximately 1 × 105 cells;

(6) Low-glucose culture environment can enhance the wound healing capability of diabetic adipose-derived stem cellsStem Cell Research & TherapySeptember 4, 2023Chun-Wei Li, Tai-Horng Young, Mu-Hui Wang, Ming-Ying Pei, Tsung-Yu Hsieh, Chia-Lang Hsu, Nai-Chen Chenggroups, respectively. Each hASC was isolated using Single-Cell Capture and cDNA Synthesis with the BD Rhapsody Express Single-Cell Analysis System (BD Biosciences, Franklin Lakes, New Jersey) following the manufacturer’s protocol, and we prepared

(7) Systemic alterations in neutrophils and their precursors in early-stage chronic obstructive pulmonary diseaseCell ReportsJune 1, 2023Theodore S. Kapellos, Kevin Baßler, Wataru Fujii, Christina Nalkurthi, Anna C. Schaar, Lorenzo Bonaguro, Tal Pecht, Izabela Galvao, Shobhit Agrawal, Adem Saglam, et al.(BD Biosciences). Single cells were isolated using Single Cell Capture and cDNA Synthesis with the BD Rhapsody Express Single-Cell Analysis System according to the manufacturer’s recommendations (BD Biosciences). cDNA libraries were prepared using

(8) Natural killer cells and innate lymphoid cells 1 tune anxiety-like behavior and memory in mice via interferon-γ and acetylcholineNature CommunicationsMay 29, 2023Stefano Garofalo, Germana Cocozza, Alessandro Mormino, Giovanni Bernardini, Eleonora Russo, Donald Ielpo, Diego Andolina, Rossella Ventura, Katiuscia Martinello, Massimiliano Renzi, et al.Single cells from the pooled sample were isolated using Single Cell Capture and cDNA Synthesis with the BD Rhapsody Express Single-Cell Analysis System following the manufacturers protocol (BD Biosciences). After priming the nanowell cartridges, the pooled

(9) Parallel Dysregulated Immune Response in Severe Forms of COVID-19 and Bacterial Sepsis via Single-Cell Transcriptome SequencingBiomedicinesMarch 3, 2023Alexis Garduno, Gustavo Sganzerla Martinez, Ali Toloue Ostadgavahi, David Kelvin, Rachael Cusack, Ignacio Martin-Loechessixteen cartridges. The single cells were isolated using single-cell capture and cDNA synthesis with the BD Rhapsody Express Single-Cell Analysis System, according to the manufacturer’s recommendations (BD Biosciences, Franklin Lakes, NJ, USA). The 399

(10) Active eosinophils regulate host defence and immune responses in colitisNatureMarch 2, 2023Alessandra Gurtner, Costanza Borrelli, Ignacio Gonzalez-Perez, Karsten Bach, Ilhan E. Acar, Nicolás G. Núñez, Daniel Crepaz, Kristina Handler, Vivian P. Vu, Atefeh Lafzi, et al.BD Rhapsody cartridges were super-loaded with 60,000 cells each. Single cells were isolated with the BD Rhapsody Express Single-Cell Analysis System according to the manufacturer’s recommendations (BD Biosciences). cDNA libraries were prepared using

(11) Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral boneFrontiers in cell and developmental biologyJanuary 1, 2023Hagizawa H, Koyamatsu S, Okada S, Kaito T, Tsumaki N.(Shichino et al., 2022). In brief, sorted single-cell suspensions were used to synthesize cDNA with BD Rhapsody Express Single-Cell Analysis System (BD Biosciences) using a BD Rhapsody Targeted and Abseq Reagent kit (BD Biosciences) following the manufacturer’s

(12) Dissecting the mechanism of cytokine release induced by T-cell engagers highlights the contribution of neutrophilsOncoImmunologyDecember 31, 2022Gabrielle Leclercq et al.000–50 000 cells. Single cells were isolated using Single-Cell Capture and cDNA Synthesis with the BD Rhapsody Express Single-Cell Analysis System according to the manufacturer’s recommendations (BD Biosciences). cDNA libraries were prepared using

(13) Single-cell multiomics revealed the dynamics of antigen presentation, immune response and T cell activation in the COVID-19 positive and recovered individualsFrontiers in ImmunologyDecember 2, 2022Partha Chattopadhyay, Kriti Khare, Manish Kumar, Pallavi Mishra, Alok Anand, Ranjeet Maurya, Rohit Gupta, Shweta Sahni, Ayushi Gupta, Saruchi Wadhwa, et al.guide (Doc ID: 214419 Rev. 2.0). An average of 30000 pooled cells were loaded in each cartridge on the BD Rhapsody express single cell analysis system for single cell capture followed by the cDNA synthesis as per manufacturer’s guideline (Doc ID: 210967

(14) Low-dose IL-2 reduces IL-21+ T cell frequency and induces anti-inflammatory gene expression in type 1 diabetesNature CommunicationsNovember 28, 2022Jia-Yuan Zhang, Fiona Hamey, Dominik Trzupek, Marius Mickunas, Mercede Lee, Leila Godfrey, Jennie H. M. Yang, Marcin L. Pekalski, Jane Kennet, Frank Waldron-Lynch, et al.preparation and sequencing Single-cell capture, and cDNA library preparation was performed using the BD Rhapsody Express single-cell analysis system (BD Biosciences), according to the manufacturer’s instructions. cDNA was initially amplified for 11

(15) Identification of the niche and mobilization mechanism for tissue-protective multipotential bone marrow ILC progenitorsScience advancesNovember 25, 2022Liu Q, Lee JH, Kang HM, Kim CH.Single-Cell Multiplexing Kit and loaded onto a BD Rhapsody cartridge. cDNA synthesis was performed using the BD Rhapsody Express Single-Cell Analysis System, and gene expression libraries were prepared using the Whole Transcriptome Analysis Amplification Kit

(16) Characteristic analysis of skin keratinocytes in patients with type 2 diabetes based on the single-cell levelsChinese Medical JournalNovember 17, 2022Bingye Liao, Qiuyi Ouyang, Hongqin Song, Ziqi Wang, Jinhua Ou, Jinxin Huang, Liang LiuSingle cells were isolated using Single-Cell Capture and complementary DNA (cDNA) Synthesis with the BD Rhapsody Express Single-Cell Analysis System, and cDNA libraries were prepared using the BD Rhapsody™ Whole Transcriptome Analysis Amplification

(17) Innate immune imprints in SARS-CoV-2 Omicron variant infection convalescentsSignal Transduct Target TherNovember 16, 2022Zhiqing Li, Xiaosu Chen, Junyan Dan, Tianju Hu, Ye Hu, Shuxun Liu, Yangyang Chai, Yansong Shi, Jian Wu, Hailai Ni, et al.a BD Rhapsody Cartridge. Single-cell capture and cDNA library preparation were performed using the BD Rhapsody Express Single-Cell Analysis System (BD Biosciences) according to the manufacturer’s instructions. Libraries of single-cell transcriptomes

(18) Immune Transcriptome Study of Human Nucleated Erythroid Cells from Different Tissues by Single-Cell RNA-SequencingCellsNovember 9, 2022Roman Perik-Zavodskii, Olga Perik-Zavodskaia, Julia Shevchenko, Vera Denisova, Saleh Alrhmoun, Marina Volynets, Valeriy Tereshchenko, Konstantin Zaitsev, Sergey SennikovPreparation and Sequencing We performed single-cell capture and cDNA library preparation using the BD Rhapsody Express Single-Cell Analysis System (BD Biosciences, Franklin Lakes, NJ, USA), in accordance with the instructions of the manufacturer.

(19) Signatures of EMT, immunosuppression, and inflammation in primary and recurrent human cutaneous squamous cell carcinoma at single-cell resolutionTheranosticsOctober 31, 2022Xin Li, Shuang Zhao, Xiaohui Bian, Lining Zhang, Lixia Lu, Shiyao Pei, Liang Dong, Wensheng Shi, Lingjuan Huang, Xiyuan Zhang, et al.Capture and cDNA Synthesis). Single-cell capture and cDNA library preparation were performed using the BD Rhapsody Express Single-Cell Analysis System (BD Biosciences), following the manufacturer's instructions. The libraries were loaded on an S1 flow

(20) Multi-omics analysis defines highly refractory RAS burdened immature subgroup of infant acute lymphoblastic leukemiaNature CommunicationsAugust 30, 2022Tomoya Isobe et al.approximately 5000 cells per were pooled and subjected to single-cell capture and cDNA synthesis with the BD Rhapsody Express Single-Cell Analysis System (BD Biosciences). Subsequently, cDNA libraries were prepared using the BD Rhapsody WTA Amplification

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BD Rhapsody™ Express Single-Cell Analysis System from BD Biosciences

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BD Rhapsody™ Express Single-Cell Analysis System from BD Biosciences

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