Chromosome differentiation and the radiation of the butterfly subgenus Agrodiaetus (Lepidoptera: Lycaenidae: Polyommatus)a molecular phylogenetic …

Martin Wiemers

Butterflies in the large Palearctic genus Agrodiaetus (Lepidoptera: Lycaenidae) are extremely uniform and exhibit few distinguishing morphological characters. However, these insects are distinctive in one respect: as a group they possess among the greatest interspecific karyotype diversity in the animal kingdom, with chromosome numbers (n) ranging from 10 to 125. The monophyly of Agrodiaetus and its systematic position relative to other groups within the section Polyommatus have been controversial. Characters from the mitochondrial genes for cytochrome oxidases I and II and from the nuclear gene for elongation factor 1 alpha were used to reconstruct the phylogeny of Agrodiaetus using maximum parsimony and Bayesian phylogenetic methods. Ninety-one individuals, encompassing most of the taxonomic diversity of Agrodiaetus, and representatives of 14 related genera were included in this analysis. Our data indicate that Agrodiaetus is monophyletic. Representatives of the genus Polyommatus ...

The karyotype of Polyommatus (Agrodiaetus) eriwanensis Forster, 1960 from the type locality (“Eriwan” [Yerevan, Armenia]) and other localities in Armenia was investigated. The number of chromosomal elements (bivalents+ multivalents) observed in male meiosis I was found to vary from 29 to 34. In individuals with n = 34, all observed elements were represented by bivalents. In other specimens, heterozygosity for different number of chromosomal fusions resulted in multivalent formation at MI stage and consequently in a lower number of recognizable chromosomal elements. We show that all karyotype peculiarities of P. (A.) interjectus de Lesse, 1960 (n = 29–32) from Turkey are similar to those in A. eriwanensis. The butterflies of these taxa have allopatric distribution and can be considered as conspecific.

See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/231217829 Chromosome differentiation and the radiation of the butterfly subgenus Agrodiaetus (Lepidoptera: Lycaenidae... Thesis · September 2003 CITATIONS READS 36 103 1 author: Martin Wiemers Helmholtz-Zentrum für Umweltforschung 92 PUBLICATIONS 1,118 CITATIONS SEE PROFILE Some of the authors of this publication are also working on these related projects: Flying Beauties View project LEGATO View project Available from: Martin Wiemers Retrieved on: 15 November 2016 Chromosome differentiation and the radiation of the butterfly subgenus Agrodiaetus (Lepidoptera: Lycaenidae: Polyommatus) – a molecular phylogenetic approach Dissertation zur Erlangung des Doktorgrades (Dr. rer. nat.) der Mathematisch-Naturwissenschaftlichen Fakultät der Rheinischen Friedrich-Wilhelms-Universität Bonn vorgelegt von Martin Wiemers aus Münster (Westf.) Bonn – September 2003 Angefertigt mit Genehmigung der Mathematisch-Naturwissenschaftlichen Fakultät der Rheinischen Friedrich-Wilhelms-Universität Bonn 1. Referent: Prof. Dr. Clas M. Naumann 2. Referent: Priv.-Doz. Dr. Bernhard Misof Tag der Promotion: _________________ 2 Contents CONTENTS CHAPTER 1: INTRODUCTION ........................................................................................................................ 5 SCOPE AND AIMS ................................................................................................................................................. 7 CHAPTER 2: NEW KARYOLOGICAL RESULTS IN AGRODIAETUS ...................................................... 9 INTRODUCTION ................................................................................................................................................... 9 MATERIAL AND METHODS ................................................................................................................................... 9 RESULTS ........................................................................................................................................................... 10 Karyotype photographs of Agrodiaetus....................................................................................................... 11 Overview of karyotypes in the subgenus Agrodiaetus Hübner, 1822 .......................................................... 15 DISCUSSION ...................................................................................................................................................... 29 CHAPTER 3: A MOLECULAR PHYLOGENY OF AGRODIAETUS ......................................................... 33 MATERIAL AND METHODS ................................................................................................................................. 33 Material ....................................................................................................................................................... 33 Selection of taxa .......................................................................................................................................... 36 DNA extraction............................................................................................................................................ 37 Selection of genes ........................................................................................................................................ 37 PCR and sequencing.................................................................................................................................... 38 Sequence alignment ..................................................................................................................................... 39 Choice of methods for phylogenetic inference............................................................................................. 40 Sequence comparisons and phylogenetic inference..................................................................................... 41 RESULTS ........................................................................................................................................................... 43 Sequences and alignment............................................................................................................................. 43 Base composition and sequence divergence................................................................................................ 43 Bayesian inference of phylogeny ................................................................................................................. 48 Maximum parsimony analysis ..................................................................................................................... 60 Statistical parsimony networks.................................................................................................................... 74 DISCUSSION ...................................................................................................................................................... 87 Comparisons with traditional systematics................................................................................................... 87 Congruence between gene trees and species trees in outgroups ................................................................. 90 Comparison with allozyme results............................................................................................................... 94 Hybridization in Agrodiaetus ...................................................................................................................... 94 Radiation of Agrodiaetus and the colonization of Europe........................................................................... 96 CHAPTER 4: SYSTEMATICS OF AGRODIAETUS BASED ON MOLECULAR EVIDENCE ............... 97 CHAPTER 5: EVOLUTION OF MORPHOLOGICAL TRAITS IN AGRODIAETUS............................. 107 INTRODUCTION ............................................................................................................................................... 107 MATERIAL AND METHODS ............................................................................................................................... 107 RESULTS ......................................................................................................................................................... 108 DISCUSSION .................................................................................................................................................... 110 SUMMARY ....................................................................................................................................................... 112 CHAPTER 6: THE ROLE OF CHROMOSOME EVOLUTION IN THE RADIATION OF AGRODIAETUS ................................................................................................................................................ 113 INTRODUCTION ............................................................................................................................................... 113 MATERIAL AND METHODS ............................................................................................................................... 113 RESULTS ......................................................................................................................................................... 114 DISCUSSION .................................................................................................................................................... 116 SUMMARY ....................................................................................................................................................... 119 SUMMARY ....................................................................................................................................................... 120 ZUSAMMENFASSUNG .................................................................................................................................. 120 ACKNOWLEDGEMENTS.............................................................................................................................. 121 REFERENCES.................................................................................................................................................. 123 HILFSMITTEL................................................................................................................................................. 143 3 Contents APPENDIX 1. KARYOLOGICAL RESULTS IN AGRODIAETUS ....................................................... 145 APPENDIX 2. LIST OF MATERIAL............................................................................................. 149 APPENDIX 3. SEQUENCE DATA ............................................................................................... 165 PLATES. AGRODIAETUS WINGS ............................................................................................... 201 4 Introduction Chapter 1: Introduction The Holarctic butterfly genus Polyommatus Latreille, 1804 consists of approximately 120-180 species (HÄUSER & ECKWEILER 1997), many of which have been placed into subgroups which are considered separate genera according to numerous authors (e.g. the subgenera Meleageria, Cyaniris, Plebicula, Sublysandra, Neolysandra). The subgenus Agrodiaetus Hübner, 1822, which is distributed in the southern Palaearctic, is the most rich in species. ECKWEILER & HÄUSER (1997) recognize between 56 and 84 species whereas BÁLINT & JOHNSON (1997) list 102 species (including 8 species placed under Paragrodiaetus Rose & Schurian, 1977). Since 1997 alone, 23 further species rank taxa have been described from Anatolia and Iran. The distribution of this subgenus ranges from the Iberian Peninsula to northern Pakistan and Central Mongolia, but the majority of species is found in Anatolia, Transcaucasia and Iran. The characteristic morphological feature of this subgenus is the almost complete reduction of the submarginal elements of the complex wing pattern on the underside of both pairs of wings, and a prominent white streak on the underside of the hind wings (although this can be missing in some cases). Secondly the larval foodplants of all Agrodiaetus species as far as they are known belong to only two closely related genera of Fabaceae, Onobrychis and Hedysarum which are only used by few other Polyommatus species. However, the relevance of these two features for a presumed monophylum Agrodiaetus has never been checked systematically, and therefore the delimitation of this taxon in literature remains contentious. HIGGINS (1975) and TOLMAN & LEWINGTON (1997) also include e.g. the taxa thersites Cantener, 1834 (larval foodplant Onobrychis), escheri Hübner, 1823 (larval foodplant Astragalus) and amanda Schneider, 1792 (larval foodplants Vicia and Lathyrus) within Agrodiaetus. BÁLINT & JOHNSON (1997) also place thersites with Agrodiaetus, but split off eight taxa (incl. glaucias (Lederer, 1871), erschoffii (Lederer, 1869) and dagmara (Grum-Grshimailo, 1888)) from Agrodiaetus and place them into Paragrodiaetus Rose & Schurian, 1977. This genus was synonymized with Agrodiaetus by HÄUSER & ECKWEILER (1997). Despite a rather up-to-date species inventory (ECKWEILER & HÄUSER 1997) the exact number of species is uncertain. The reasons are threefold. First, this is the only Palaearctic group of butterflies in which a high number of new species is still being discovered, especially in remote parts of Iran. 15% of the almost 250 nominal taxa described in Agrodiaetus (Fig. 1) have been named the last five years, 2/3 of them of species rank. Taxa descriptions in Agrodiaetus 250 200 150 100 Taxa descriptions Total 50 0 1763 1803 1843 1883 1923 1963 2003 Period Fig. 1. Taxa descriptions in Agrodiaetus within periods of 20 years 5 Chapter 1 Secondly, many taxa represent allopatric populations which differ only slightly in morphology or karyology (morphospecies and karyospecies) and a decision on their status as distinct species or subspecies is controversial and subjective. Thirdly and most importantly many Agrodiaetus taxa are extremely similar in phenotype (HESSELBARTH et al. 1995) and in contrast to other Lepidoptera taxa genitalia offer only few distinctive features. These slight differences can only be discovered by careful and time consuming preparation techniques, the results of which have been published only for some Greek taxa (COUTSIS 1986; WAKEHAMDAWSON & SPURDENS 1994). The discovery of different chromosome numbers in allopatric populations of various Agrodiaetus taxa has lead to the description of numerous “karyospecies” which hardly differ in phenotype. About half of all Agrodiaetus species were described within the last 40 years and differences in chromosome numbers have often been the main or even the only argument for the specific separation of taxa. Currently most authors assume that major differences in chromosome numbers usually lead to reproductive isolation of taxa although experiments in Saturniidae have shown that interspecific crossings between different karyospecies can lead to fertile offspring (NAGARAJU & JOLLY 1986). Variation in chromosome numbers between individuals or populations are also known in many other groups of animals, e.g. in the Polyommatus subgenus Lysandra (DE LESSE 1960a & 1969), in some ancestral genera of neotropical Nymphalidae (BROWN et al. 1992), in the ant genus Leptothorax (LOISELLE et al.1990), in the Australian grasshopper Caledia (GROETERS & SHAW, 1992 & 1996; MORAN & SHAW 1977; SHAW 1976), in the New Zealand stick insect Hemideina (MORGAN-RICHARDS 1997), in the Japanese daddy longleg Gagrellopsis nodulifera (TSURUSAKI et al. 1991), in Australian scorpions of the genus Urodacus (SHANAHAN 1989) or the African rodent Otomys irroratus (CONTRAFATTO et al. 1992). Although karyological studies in the subgenus Agrodiaetus have been important to discover species which are not or only slightly differentiated morphologically, these studies have also lead to a profusion of names and increased the chaos in the systematics of this group. The difficulties in uniting Agrodiaetus species into monophyletic units at or above the species category stem from the small number of usable characters and the high level of homoplasy in most of them. Not surprisingly, all systematic treatments of this group (FORSTER 1956-1961; HESSELBARTH et al. 1995; ECKWEILER & HÄUSER 1997; BÁLINT & JOHNSON 1997) have remained highly unsatisfactory. Molecular methods appear very promising to unravel the relationships within Agrodiaetus in order to understand more about its origin and radiation. Such knowledge can also help to elucidate the karyotype evolution and biogeography of Agrodiaetus. Until now, only one such study on allozyme variation in 11 taxa, most of them from the Mediterranean region, has been published (MENSI et al. 1994). In Lycaenidae, only a few molecular studies have been conducted, which is apparent from the small number of sequences in GenBank (6 on 22/09/98 when this project started; 176 on 9/9/2003, compared to 1857 in Nymphalidae, a family with approximately the same number of species worldwide (ca. 5000) as in Lycaenidae). One of the few major ones is the thesis of MEGENS (2002) who used molecular markers (COI, COII, wingless, 12s & 16s) to infer the phylogeny of the Southeast Asian butterfly genus Arhopala. This case parallels Agrodiaetus with its high number of morphologically very similar species. Molecular data suggested a rapid radiation which started already in the Miocene (7-11 Million years ago) and was completed before the onset of the Pleistocene period. Thus glaciation events (e.g. the periodical flooding of the Sunda plateau) did not appear to be the key factors for this radiation as previously suggested. Instead, life-history parameters, i.e. shifts in larval foodplants and symbiosis with ants were assumed to have played a major role in creating the diversity of Arhopala species. 6 Introduction Life history parameters might also have been important in the radiation of Agrodiaetus. Although it is thought that the larvae only feed on two genera of Fabaceae (Onobrychis and Hedysarum), the food plant of most species is unknown. Even the few existing foodplant records can only be evaluated with great caution for various reasons: 1. Females of Agrodiaetus are often impossible to identify in the field. Therefore egg-laying records are prone to misidentification of the Agrodiaetus species. 2. Some Agrodiaetus species do not lay their eggs directly onto the foodplant but on dry plant material next to the foodplant (SCHURIAN 1976; 1999) which can easily result in erroneous records. 3. The larvae of most Agrodiaetus species are unknown and those which are known are very similar morphologically. If larvae are collected in the field they need to be reared until the eclosure of the adult butterfly for identification purposes. Apart from some European species (BOLOGNESI 2000), Agrodiaetus larvae have almost never been reared successfully. The main reason is that almost all Agrodiaetus are monovoltine, overwinter as small larvae and occur in areas with a continental climate much different from the Central European climate where most lepidopterists reside. The foodplants are also difficult to cultivate. 4. The plant genera Onobrychis and Hedysarum are very rich in species and taxonomically they pose the same puzzle to the botanist as Agrodiaetus to the zoologist. Without the help of specialist botanists, most identifications of larval foodplants will be erroneous. In contrast to many other genera of Lycaenidae which are associated with ants in a sometimes very specific way, Agrodiaetus caterpillars seem to be only tended unspecifically by ants (facultative myrmecophily, v. FIEDLER 1995c). However, hardly anything is known about ant associations in this subgenus. The only way to increase the poor knowledge on its life-history would be intensive field studies such as those carried out by DANTCHENKO (1997) on two Russian taxa. Unfortunatly there are hardly any lepidopterists in the centre of Agrodiaetus distribution who could carry out such studies. Scope and aims In the course of this thesis the following issues and questions will be addressed: Chapter 2 presents the results of the karyological studies and provides data for many taxa whose karyotype was unknown previously. It includes a synopsis of all available literature data, much of it published in many different small entomological journals which are difficult to access. The molecular genetic approach in Chapter 3 is the core of the thesis. Mitochondrial and nuclear DNA of the vast majority of Agrodiaetus species is analyzed by different methods (Maximum Parsimony, Bayesian, network) to infer the origins of and the phylogenetic relationships within Agrodiaetus. Results will be compared to current systematics which is mainly based on features of the adult phenotype. Here I will also address the question of hybridization in Agrodiaetus: Does it occur frequently causing extensive gene flow between different Agrodiaetus taxa? Another point of discussion are biogeographical issues: Where are the origins of Agrodiaetus and how old is its radiation? When was Europe colonized and how many times? Did Agrodiaetus recolonize Europe after the last glaciation or did they survive in South European refugia? Chapter 4 aims to revise the systematics of Agrodiaetus on the basis of the molecular results, taking into account karyological data and morphology. 7 Chapter 1 The relevance of morphological features for the phylogeny of Agrodiaetus will be reevaluated on the basis of the molecular phylogeny in Chapter 5: Is the white streak an autapomorphy of Agrodiaetus? Do the monomorphic brown Agrodiaetus form a monophyletic unit? Is the development of androconial patches correlated with the loss of iridescent coloration does it thus replace a visual sexual communication system with an olfactorial one or is it independent of wing coloration? Chapter 6 discusses the karyological results in the light of the new phylogenetic framework: Do we find phylogenetical or biogeographical patterns in chromosome number variation? Is chromosomal evolution a directional process towards increasing numbers caused by fission or decreasing numbers by repeated fusion events? Are such events confined to certain clades? Can low and/or high chromosome numbers even have an adaptive value? Do chromosomal changes occur as byproducts of speciation or are they the cause of it? Do we even find indications that speciation occurred without a biogeographical barrier, and that chromosomal mutations provide sufficient reproductive isolation for new species to evolve in sympatry? 8 New karyological results in Agrodiaetus Chapter 2: New karyological results in Agrodiaetus Introduction A peculiarity of Agrodiaetus is the incredible variation in chromosome numbers ranging from 10 to 130 for the haploid set. Currently the chromosome numbers of about 65% of all Agrodiaetus species are known. The fundamental works were done in the 1950s and 60s by DE LESSE (1952, 1957, 1959a-f, 1960a-c, 1961a-c, 1962a-b, 1963a-c, 1964, 1966) and in recent years by LUKHTANOV (LUKHTANOV 1989, HESSELBARTH et al. 1995, KANDUL & LUKHTANOV 1997, LUKHTANOV et al. 1997, 1998, LUKHTANOV & DANTCHENKO 2002a-b), besides BROWN (1976b, 1977), BROWN & COUTSIS (1978), COUTSIS et al. (1999), LARSEN (1974, 1975), MUNGUIRA et al. (1995), OLIVIER et al. (1999a-b, 2000), TROIANO et al. (1979) and TROIANO & GIRIBALDI (1979). The current knowledge is best of species from Europe and Southwest Asia whereas the chromosome number of most Central Asian species (which represent less than 30% of the total number of species) is still unknown. The haploid chromosome numbers range from n=10-11 in A. birunii Eckweiler & ten Hagen, 19981 to n=128-131 in A. shahrami Skala, 2001. Compared to the modal value of n=24 in Lycaenidae (LORKOVIĆ 1990), which is only found in very few Agrodiaetus species (like A. poseidonides (Staudinger, 1886) and A. turcicus (Koçak, 1977)) these numbers represent either a reduction (less than half) or a multiplication (up to a quintuple) of the probably plesiomorph modal chromosome number of Lycaenidae. The only Lepidoptera species where even higher numbers are known are the closely related Polyommatus dorylas (n=149-151), P. nivescens (n=190-191) from Spain and finally P. atlantica (n=221-223) from Morocco which has the highest chromosome number known in Metazoa. The mechanism which leads to the change in chromosome numbers is unknown, but it seems most probable that low chromosome numbers are caused by fusion and high numbers by fission of chromosomes (LORKOVIĆ, 1990). This is also indicated by the striking reciprocal correlation between the number of chromosomes and their size. Polyploidy, which was recently suggested as a possible mechanism in some Coleophora species (LUKHTANOV & PUPLESIENE 1999), is extremely unlikely in Agrodiaetus. Material and methods Material available for karyological studies is listed in Appendix 2. There was a total of 1155 chromosome fixations available for study (976 Agrodiaetus and 179 other specimens of Lycaenidae). These were fixed in the field by placing the testes or the posterior two thirds of the abdomens of freshly killed butterflies into Eppendorf vials (0.5ml) with a freshly prepared mixture of 100% Ethanol and 100% Acetic Acid (3:1). The vials were kept in ice water during travel and later on stored in the lab at +7°C. Karyological preparations were made with squash techniques according to the protocols in OLIVIER et al. (2000) or LUKHTANOV & DANTCHENKO (2002a) and carried out in cooperation with Dr. Jurate De Prins (Antwerpen), Karen Meusemann (Bonn) and Dr. Vladimir Lukhtanov (St. Petersburg). Digital images of preparations were obtained with an Olympus DP-50 digital camera attached to a Leica Aristoplan microscope using the software package “Analysis”. The number of chromosomes was counted and peculiarities of the karyotype were recorded. A total of 624 preparations were made (586 Agrodiaetus and 38 other species of Lycaenidae). 1 The even lower numbers of n=8-11 recorded for A. nephohiptamenos (Brown & Coutsis, 1978) have turned out to be erroneous (J. DE PRINS, pers. comm.) like those for A. aroaniensis (Brown, 1976), v. COUTSIS et al. (1999). 9 Chapter 2 Results Meiotic cells with countable chromosome numbers were found in 282 preparations (45%) and chromosome counts are given in Appendix 1. The karyotype of many species was previously unknown (see discussion). A selection of karyotype photos of such species and of those specimens which are of special scientific interest are presented here (Tab. 1; Figs. 2-27). Tab. 1. List of figured karyotypes Species Agrodiaetus actis Agrodiaetus ainsae Agrodiaetus birunii Agrodiaetus caeruleus Agrodiaetus dizinensis Agrodiaetus ernesti Agrodiaetus erschoffii Agrodiaetus femininoides Agrodiaetus gorbunovi Agrodiaetus iphicarmon Agrodiaetus iphigenia Agrodiaetus (damocles) kanduli Agrodiaetus karindus Agrodiaetus klausschuriani Agrodiaetus lycius Agrodiaetus mofidii sorkhensis Agrodiaetus peilei Agrodiaetus pierceae Agrodiaetus phyllis Agrodiaetus posthumus Agrodiaetus pseudoxerxes Agrodiaetus sertavulensis Agrodiaetus valiabadi Agrodiaetus zarathustra Chromosome number n=17 n=ca 108 n=10-11 n=20 n=17 n=18 n=13-14 n=27 n=20 n=29 n=15 n=25 n=68 n=56 n=22 n=ca 45 n=39 n=22 n=82-86 n=ca 85 n=15 n=20 n=23 (2n=46) n=ca.22 * Specimen codes refer to those used in Appendix 2. 10 Figure 2 3 4 5 6&7 8 9 11 10 12 13 14 15 16 17 27 18 19 20 21 22 25 23 & 24 26 Specimen codes* MW98166 MW01004 MW00267 MW00335 MW00539 MW98097 MW00393 WE02671 MW00129 MW98104 MW98106 MW99465 WE02611 MW00262 MW98069 WE02453 WE02593 MW99416 MW00348 MW00347 MW00330 MW98305 MW00498 WE02531 New karyological results in Agrodiaetus Karyotype photographs of Agrodiaetus Fig. 2. A. actis, n=17 (MW98166) Fig. 3 Agrodiaetus ainsae, n=ca 108 (MW01004) Fig. 4. Agrodiaetus birunii, n=10 (MW00267) Fig. 6. Agrodiaetus dizinensis, n=17 (MW00539) Fig. 5. Agrodiaetus caeruleus n=20 (MW00335) Fig. 7. Agrodiaetus dizinensis, 2n=34 (MW00539) 11 Chapter 2 Fig. 8. Agrodiaetus ernesti, n=18 (MW98097) Fig. 9 Agrodiaetus erschoffii n=13-14 (MW00393) Fig. 10. Agrodiaetus gorbunovi, n=20 (MW00129) Fig. 11 Agrodiaetus femininoides, n=27 (WE02671) Fig. 12. Agrodiaetus iphicarmon, n=29 (MW98104) 12 Fig. 13. Agrodiaetus iphigenia, n=15 (MW98106) New karyological results in Agrodiaetus Fig. 14. Agrodiaetus (damocles) kanduli n=25 (MW99465) Fig. 16. Agrodiaetus klausschuriani n=56 (MW00262) Fig. 18. A. peilei, n=39 (WE02593) Fig. 15. Agrodiaetus karindus, n=68 (WE02611) Fig. 17. Agrodiaetus lycius n=22 (MW98069) Fig. 19. A. pierceae, n=22 (MW99416) 13 Chapter 2 Fig. 20. A. posthumus, n= ca. 85 (MW00347) Fig. 21. A. phyllis, n=82-86 (MW00348) Fig. 22. A. pseudoxerxes n=15 (MW00330) Fig. 23. A. valiabadi n=23 (MW00498) Fig. 24 Agrodiaetus valiabadi 2n=46 (MW00498) Fig. 25. Agrodiaetus sertavulensis n=20 (MW98305) 14 Fig. 26. Agrodiaetus zarathustra n=ca 22 (WE02531) New karyological results in Agrodiaetus Fig. 27. Agrodiaetus mofidii sorkhensis, n=ca 45 (WE02453) The following list includes all species level taxa of Agrodiaetus which are included in the molecular study, even if no own karyological data are available. This list summarizes the current knowledge on their karyotypes. At this stage I adopt a splitter’s attitude and consider any taxon which might represent a separate species, whether based on different morphology or karyology and including allopatric taxa which might only represent subspecies of another taxon. An evaluation of their status will be done later in the course of this thesis, taking into account the results of the molecular genetic studies. Overview of karyotypes in the subgenus Agrodiaetus Hübner, 1822 admetus-group (grouping according to ECKWEILER & HÄUSER, 1997) This is a group of sexually monomorph brown Agrodiaetus taxa which are often difficult or even impossible to identify without the knowledge of their karyotypes. admetus (Esper, [1783]) This species which is distributed from Hungary (type locality) to Siberia is one of the few exceptions and is easy to identify. A haploid chromosome number of n=80 is known from populations in Bulgaria, Western Anatolia and Armenia, and slightly lower numbers (n=7779) from Eastern Anatolia (DE LESSE 1960b; LUKHTANOV & DANTCHENKO 2002a). One chromosome is larger and the others are gradually decreasing in size. We can confirm this karyotype with a chromosome number of ca. n=78-80 from three preparations from Antalya and Adana Province (Turkey), the two smallest chromosomes being minute in size. 15 Chapter 2 ripartii (Freyer, 1830) This species is known to have a constant chromosome number of n=90 throughout its vast range. Chromosome counts are known from Northern Spain, France (close to the type locality Digne), Greece, Turkey and Kazakhstan (DE LESSE 1960a, 1960b, 1961b, 1961c; LUKHTANOV & DANTCHENKO 2002a, 2002b; COUTSIS et al. 1999). There is one large and one medium sized chromosome while the others are all of similar small size. We were not able to determine the exact chromosome number in any specimen but counts around n=90 were obtained from populations in Spain (Burgos) and Turkey (Isparta, Adana, Artvin, Erzincan & Van). DE LESSE (1960b) remarks that most specimens from Turkey (although fresh) only had atypical divisions. We had the same problem and found typical divisions only in one fifth of the 17 preparations from Turkey. fabressei (Oberthür, 1910) According to DE LESSE (1960a, 1960b, 1961b) and MUNGUIRA et al. (1995) this Spanish taxon which replaces ripartii in Castile has the same chromosome number as ripartii (n=90) but a different number of large chromosomes, two large and two medium ones according to the first author, but three “macrochromosomes” according to the second author team. The metaphases in our preparations were not clear enough to establish the exact number of chromosomes or “macrochromosomes” but in cases of species with chromosomes gradually decreasing in size it is hardly possible to state a certain number of macrochromosomes. This is especially true for squash preparations (as done by MUNGUIRA (l.c.) and us), because the size depends also on how chromosomes were spread when pressing the cover slide. DE LESSE (1961d) found a contact zone of fabressei and ripartii in Teruel but both taxa have not been found sympatrically (DE LESSE 1968; MUNGUIRA et al. 1995). humedasae (Toso & Baletto, 1976) This endemic of Aosta valley (Italy) has a chromosome number of n=38 (TROIANO et al. 1979). We counted approximately the same number of chromosomes. aroaniensis (Brown, 1976) The chromosome number of this Greek taxon is n=48 (COUTSIS et al. 1999), not 15-16 as recorded by BROWN (1976b). No fixations were available to us. nephohiptamenos (Brown & Coutsis, 1978) The chromosome number of this Greek taxon was recorded as n=8-11 (BROWN & COUTSIS 1978), which would constitute the lowest number in Agrodiaetus, but according to DE PRINS (pers. comm.), the species has a very high chromosome number, possibly n=90 as in ripartii. No fixations were available to us. alcestis (Zerny, 1932) With n=19-21, this taxon has the lowest number of chromosomes of all brown Agrodiaetus (DE LESSE 1960A, 1960B; LARSEN 1975; LUKHTANOV et al. 1998; LUKHTANOV & DANTCHENKO 2002B). A haploid number of n=19 was found in Iran and Southeast Turkey, n=20(-21) in other Turkish provinces and Lebanon. Our results from Turkey and Iran confirm these results. The ssp. karacetinae Lukhtanov & Dantchenko, 2002 has been described recently from Dez valley (Hakkari) and Iran for the populations with a karyotype of n=19 chromosomes. demavendi (Pfeiffer, 1938) Variable chromosome counts of n=66-76 are known for this monomorphic Agrodiaetus species from various provinces in Iran, Turkey and Armenia (DE LESSE 1960A, 1960B; 16 New karyological results in Agrodiaetus LUKHTANOV et al. 1998; LUKHTANOV & DANTCHENKO 2002A) and our results from East Turkey and Northwest Iran fall within this range. DE LESSE (1960b) also notes the higher number of larger chromosomes (two large and several medium ones) in comparison with ripartii with only one large and one medium-sized chromosome. From the excellent photographs of LUKHTANOV & DANTCHENKO (2002) it is apparent that the chromosomes of demavendi gradually decrease in size. Populations from Kopet Dagh (Iran) with an even higher chromosome number of n=84 (DE LESSE 1963a) have been separated as khorasanensis (Carbonell, 2001). Our material from Tehran (Samqabad) is referable by external features to the newly described taxon ahmadi (Carbonell, 2001) from Zanjan and some specimens from Azarbayjan-e Sharqi are similar to the newly described taxon urmiaensis (Schurian & ten Hagen, 2003) from Urmia Lake (Northwest Iran). Although the ranges of demavendi and ripartii overlap in Eastern Anatolia a sympatric occurrence of these two taxa, verified karyologically, is not known. interjectus (de Lesse, 1960) This taxon from Eastern Turkey has been described only on the basis of its karyotype (n=2932) and subsequent authors have doubted its status as a distinct species (HESSELBARTH et al. 1995; ECKWEILER & HÄUSER 1997). According to DE LESSE (1960b) it occurs sympatrically with alcestis and demavendi near Pertek. We were able to find a specimen close to the type locality near Erzincan which turned out to have n=31, confirming the results of DE LESSE (1960b). dantchenkoi Lukhtanov & Wiemers, 2003 This is a newly discovered brown Agrodiaetus species discovered in Van (Southeast Turkey) with a chromosome number of n=40-42 (LUKHTANOV, WIEMERS & MEUSEMANN, in print). Specimens MW99274, MW99319 and MW99320 constitute paratypes. Externally and genetically this taxon is similar to eriwanensis (Forster, 1960) from Armenia, which has a chromosome number of n=28-35 (LUKHTANOV & DANTCHENKO, 2002a). valiabadi (Rose & Schurian, 1977) The karyotype of this local taxon from valleys of Northern Elburs (Iran) was previously unknown. We were able to establish the chromosome number as n=23. dolus-group mithridates (Staudinger, 1878) Variable chromosome numbers of n=21-27 have been recorded from different parts of its range in Turkey (DE LESSE 1960a, 1960b). Our result of n=23 from Malatya corresponds exactly with the result of DE LESSE (1960b) from the same place. peilei Bethune-Baker, 1921 The chromosome number of this peculiar golden brown Agrodiaetus from Lorestan (Iran) was unknown previously. We were able to determine it from three specimens as n=39. antidolus (Rebel, 1901) This is the first of a group of four apparently closely related allopatric species which is known from Eastern Turkey and has a chromosome number of n=40-41 with chromosomes gradually decreasing in size (DE LESSE 1961b; HESSELBARTH et al., 1995; LUKHTANOV et al. 1998). The taxon has been described from Kazikoparan (Iğdır Prov.) and chromosome counts are known from Pertek (Tunceli), Çatak (Van) and Bagishli (Hakkari). We tried to find the species at the 17 Chapter 2 type locality without success but we can confirm the chromosome results for four populations from the province of Hakkari. kurdistanicus (Forster, 1961) Apparently this species is indistinguishable from the former taxon by external features, but its chromosome number is higher, n=61 was recorded from Görentach Köyu N of Van (DE LESSE (1960a) and n=ca. 57-62 from Çatak (Van Province) by DE LESSE (1960a) & LUKHTANOV et al. (1998). Interestingly the latter authors also found one specimen with antidolus karyotype at Çatak. We obtained similar chromosome counts from Çatak and Erek Dağı (Van Province), with one precise result from Çatak (n=62). Erek Dağı is the type locality of kurdistanicus. Although we did not obtain precise counts from this locality we can confirm that the karyotype with high chromosome number occurs at the type locality. This is important for the stability of the name kurdistanicus because antidolus populations with low chromosome number karyotype occur in close proximity. morgani (Le Cerf, 1909) The chromosome number of this taxon from Iranian Kordestan was determined as n=25-26 by DE LESSE (1961b) from a population near Sanandaj. Our result (n=27 from Saqqez) is very close to this figure. femininoides (Eckweiler, 1987) The chromosome number of this darkest member of the antidolus species group from Northwest Iran was unknown until now. We were able to determine its chromosome number from two populations in Zanjan and Ardabil with n=27 to be the same as in morgani. sennanensis (De Lesse, 1959) The chromosome number of this taxon from Iranian Kordestan which was described as a subspecies of hopfferi was established from the type series (including the holotype from Hamakasi, Sanandaj) as n=28-30 (DE LESSE 1959c). No fixations were available to us. menalcas (Freyer, [1837]) The chromosome number of this Turkish taxon was recorded to be stable with n=85 in several populations throughout Turkey (DE LESSE 1960a, 1961a). Our results (the first ones from Fethiye and Van Province) confirm these results and we can also confirm that two of these chromosomes are much larger than the other ones (“macrochromosomes”). dolus (Hübner, [1823]) We did not have material from Southern France where DE LESSE (1960a, 1961a, 1962b) recorded a chromosome number of n=123-125, the second highest number known in Agrodiaetus, but the following two Spanish taxa are very closely related. It should be noted that the type locality of dolus is unknown and could be Southern France (as often suggested), Italy or Spain. ainsae (Forster, 1961) This taxon represents dolus in Northern Spain and was separated from it because of its lower chromosome number of n=108-110 (DE LESSE 1962b; MUNGUIRA et al. 1995). We can confirm this chromosome number. According to MUNGUIRA et al. (1995) ainsae has two “macrochromosomes” compared to six in fulgens which is the reason to consider these allopatric taxa distinct species. This number is based on a single specimen from Burgos of which the number of chromosomes could not be established due to the poor quality of the preparation and probably for this reason it is also not figured. We obtained good metaphase 18 New karyological results in Agrodiaetus plates of two specimens from Illarduya (Alava) which display 108 chromosomes gradually decreasing in size, about six of them could be called “macrochromosomes” according to MUNGUIRA et al. (1995). DE LESSE (1962b) described a new subspecies (A. dolus pseudovirgilia) from Burgos, only based on the small wing size and slight differences in coloration. fulgens (de Sagarra, 1925) This taxon from Catalonia was separated by MUNGUIRA et al. (1995) from ainsae due to the higher number of “macrochromosomes” (six compared to two in ainsae) while the total number of chromosomes was found to be very similar (n=103, judged from three specimens). As is shown in the previous paragraph (under ainsae) this difference in “macrochromosomes” does not really exist. It should also be noted that a precise count of such high chromosome numbers and the identification of an exact number of macrochromosomes which gradually decrease in size is hardly possible when using the squash technique. DE LESSE (1966) already mentioned that (also with his technique) higher numbers of good metaphase plates are necessary to establish the exact chromosome number in species with such a high number of chromosomes. This taxon cannot be separated from ainsae by external characters either. We did not get precise counts from our metaphase plates because some chromosomes seem to overlap but the chromosome number of fulgens must be between n=100 and n=110. dama-group dama (Staudinger, 1892) This South Anatolian species has only been found in a few localities in Malatya, Maraş and Mardin Province (Turkey). DE LESSE (1959f) described its karyotype from Kahramanmaraş and OLIVIER et al. (1999b) confirmed his results from the type locality Malatya. It has an asymmetric karyotype with n=41 chromosomes, about eleven of them are large, gradually decreasing in size, the others medium-sized. hamadanensis (de Lesse, 1959) The chromosome number of this Iranian taxon was found by DE LESSE (1959a) to be n=21-22 in two populations from the type locality (Araj Pass) and from Sanandaj (Kordestan). We can confirm these results for two populations from Tehran province and a population from Ardabil (Azarbayjan-e Sharqi Province). karindus (Riley, 1921) The karyotype of this Iranian taxon was previously unknown and we were able to determine the chromosome number as n=66-68 for two specimens from Saqqez (Kordestan). This taxon has been treated as a subspecies of dama (Staudinger, 1892) which is only known from Malatya (Turkey), but the latter has a chromosome number of n=41-42 (DE LESSE 1959f; OLIVIER et al. 1999). theresiae Schurian, van Oorschot & van den Brink, 1992 The karyotype of this taxon which was described from Saimbeyli in Adana Province (Turkey) was recorded as n=41-42 (HESSELBARTH et al. 1995; KANDUL & LUKHTANOV 1997), but the material came from another population near Taşkent (Konya Province). OLIVIER et al. (1999) proved with our material (specimens MW98240, MW98241, MW98243) that the chromosome number of true theresiae is n=63. The population from Taşkent was described accordingly as the following new species. 19 Chapter 2 guezelmavi Olivier, Puplesiene, van der Poorten, De Prins & Wiemers, 1999 Separated from theresiae on the basis of its karyotype which is n=41-42 (KANDUL & LUKHTANOV 1997; OLIVIER et al. 1999) and only known from its type locality (Taşkent, Konya Prov., Turkey). Our material confirms these results. Externally this taxon cannot be separated from the allopatric theresiae. damon-group hopfferi (Herrich-Schäffer, [1851]) For this Anatolian species a stable chromosome number of n=15 was recorded by DE LESSE (1959c, 1959f, 1960a) and LUKHTANOV et al. (1998) from different Turkish Provinces. We can confirm these results for Malatya, Van and Hakkari Province. lycius (Carbonell, 1996) This recently discovered species was only known from the type locality (Elmali in Antalya Province, Turkey) until a further population was discovered on the Turkey expedition 1998 near Güneykent (Isparta Province). Its haploid chromosome number was only approximately determined by CARBONELL (1996) as ca. 20-25. We can specify this number more precisely for three specimens from the type locality with n=21-22. poseidon (Herrich-Schäffer, [1851]) DE LESSE (1963c) recorded different chromosome numbers from different localities: n=19 (Pozantı, Adana Prov.), n=20 (Kahramanmaraş) and n=21-22 (Amasya). KANDUL & LUKHTANOV (1997) recorded n=19 from Yusufeli (Artvin) and OLIVIER et al. (1999) n=20 from Malatya. We also obtained different results for populations from Zelve (Nevşehir) with n=20-21 and from Gökpinar (Sivas) with n=19. A clear geographical pattern does not emerge from these results. In one population from Ağrı DE LESSE (1963c) found a much higher chromosome number (n=24-26) but did not describe it as a new taxon. Only recently it was named as the following new karyospecies: putnami (Dantchenko & Lukhtanov, 2002) This taxon was separated from poseidon and described as a new species because of its different karyotype. The holotype from Kayabaşı (Prov. Erzurum) had a haploid chromosome number of n=26 and 4 males from Ağrı (2.VIII.1958, De Lesse leg., n=24-27) were included in the type series. We checked material from Ağrı and found n=25 in one specimen, thus specifying the results of DE LESSE (1963c). In another specimen from Yusufeli (Artvin) the chromosome number could be only approximately established as n=23-25. This specimen was attributed to putnami by LUKHTANOV (pers. comm.). If this is correct, both the karyospecies putnami and poseidon would occur in sympatry at Yusufeli, but in the author’s opinion the plates are not clear enough to rule out a lower number of only n=21 chromosomes as found in poseidon. damocles (Herrich-Schäffer, [1844]) Until recently, this species was only known from the South Ural Mts. (ssp. damocles (Herrich-Schäffer, [1844]), the Crimean Peninsula (ssp. krymaeus (Sheljuzhko, 1928) with n=25-27 according to KANDUL & LUKHTANOV (1997)) and the Volga river (ssp. rossicus Dantchenko & Lukhtanov, 1993) with n=24-26 according to LUKHTANOV et al. 1997). LUKHTANOV & DANTCHENKO (2002b) recorded this species for the first time from Turkey and described it as a new subspecies of damocles: 20 New karyological results in Agrodiaetus kanduli (Dantchenko & Lukhtanov, 2002) This taxon was described as a subspecies of damocles (Herrich-Schäffer, [1844]). The chromosome number of the holotype from Yildiz (Erzincan, Turkey) was determined as n=25. Three other males from the same locality were included in the type series but without karyological data. Specimens with similar phenotype have been found before in Eastern Turkey but were assigned to wagneri (HESSELBARTH et al., 1995). We found one specimen (MW99465) near Çatak (Van Province) which resembles this taxon closely in its phenotype and which turned out to have the same karyotype (n=25). It was assigned to damocles by LUKHTANOV (pers. comm.). caeruleus (Staudinger, 1871) The karyotype of this species was unknown until now. We were able to determine a haploid chromosome number of n=20 from two specimens of both type localities, Schakuh and Hajiabad (Prov. Golestan, Iran). transcaspicus (Heyne, [1895]) A haploid chromosome number of n=52-53 has been recorded by DE LESSE (1963a, 1963c) for this species from Kopet Dagh. The following six taxa are very similar to it in phenotype but have very different chromosome numbers: elbursicus (Forster, 1956) DE LESSE (1963c) checked material from the type locality of this taxon at Kendevan Pass (Elburs, Iran) and recorded a haploid chromosome number of n=16-17. Our results from four populations around Kendevan Pass (n=16-18) confirm these results. We also obtained a result of one specimen similar to elbursicus from Zanjan with n=18. A slightly higher chromosome number of n=19-20 was found by DE LESSE (1963c) in populations from the Eastern Elburs Mts. from Āb Ali (Demavend) and Firuzkuh. zapvadi (Carbonell, 1993) This taxon was described as a subspecies of elbursicus from Güzelsu (Van Province, Turkey) with a haploid chromosome number of n=18-19 (DE LESSE 1963c). LUKHTANOV et al. (1998) recorded a chromosome number of n=17-18 from the type locality and we can confirm a number of n=18-19 for the same locality. HESSELBARTH et al. (1995) synonymized this taxon with elbursicus. ninae (Forster, 1956) The karyotype of this taxon was recorded to be n=34 in Armenia near the type locality (Daralagez Mts.) by LUKHTANOV (1989) and LUKHTANOV & DANTCHENKO (2002a) and n=33-37 in Northeast Turkey (Doğubayazıt, Ararat, Ağrı) by DE LESSE (1963c). Chromosomes are gradually decreasing in size. Our result of a specimen from Ağrı (n=34) confirms these figures. turcicola (Koçak, 1977) This taxon was described as a subspecies of transcaspicus from Van (Turkey). Its phenotype seems to be indistinguishable from the allopatric ninae but its chromosome number is n=(19-)20 (DE LESSE 1960a, 1963c; LUKHTANOV et al. 1998). Our results confirm a chromosome number of n=20 for three populations in Van Province but one specimen from Çatak had a higher number (n=22) which is the same as in the closely related aserbaidschanus (Forster, 1956) from Azarbaijan. Sympatric occurrence with zapvadi has been reported from many locations in Van Province, and at one of them (Güzelsu) 21 Chapter 2 LUKHTANOV et al. (1998) reported a chromosome number of n=19-20 compared to n=17-18 for zapvadi at the same location. huberti (Carbonell, 1993) This species was separated from ninae based on slight differences in phenotype while the karyotype is hardly different. A chromosome number of n=33-34 was recorded by DE LESSE (1963c) from the type locality at Ağrı and LUKHTANOV & DANTCHENKO (2002a) recorded n=35-37 for Armenia. We obtained chromosome counts of n=33-36 from populations in Northeast Turkey (Artvin, Kars & Bayburt Provinces). pierceae (Lukhtanov & Dantchenko, 2002) LUKHTANOV et al. (1998) discovered that huberti from Güzeldere Geçidi (Van Prov.) has a divergent karyotype with n=22 and therefore it was described as a distinct species four years later. We obtained chromosome counts of n=ca.22 also from Çatak (Van Prov.) and Dez valley (Hakkari). carmon (Herrich-Schäffer, [1851]) DE LESSE (1960a, 1963b) investigated material of this Anatolian species from the type locality (Amasya) and from Gümüşhane and Kayseri and found a haploid chromosome number of n=81-82. One chromosome is much larger than all the other ones. We obtained only an approximate result from Artvin Province which is similar (n>=79) while two specimens from Antalya Province gave no results at all. schuriani (Rose, 1978) This taxon was described as a subspecies of carmon from Nevşehir but sunk into synonymy by HESSELBARTH et al. (1995). We found a specimen at Gezbeli Geçidi (Kayseri) which might represent this taxon and the chromosome number was only approximately identified as n=75-80 which does not exclude the possibility that the true number is n=81-82 as established by DE LESSE (1963b) from Kayseri. surakovi Dantchenko & Lukhtanov, 1994 This taxon was described as a subspecies of carmon from Azarbaijan but later raised to species status because of its different karyotype with n=50 recorded from three Armenian populations. The chromosomes gradually decrease in size (LUKHTANOV & DANTCHENKO 2002a). LUKHTANOV & DANTCHENKO (2002b) recorded this species also from Turkey and named it as a different subspecies: sekercioglui (Dantchenko & Lukhtanov, 2002) Described from Çatak (Van Province, Turkey) as a subspecies of surakovi with the same chromosome number of n=50 (LUKHTANOV & DANTCHENKO 2002b). We obtained only an approximate chromosome count from one specimen collected near the type locality which is close to that figure. pseudoxerxes (Forster, 1956) This species was described as a subspecies of carmon from Schakuh in Eastern Elburs Mts. (Iran) and at the same time kendevani (Forster, 1956) was described as another subspecies of carmon from Kendevan Pass in Central Elburs Mts. (Iran). DE LESSE (1962a) ruled out conspecifity of both taxa (as recently suggested again by ECKWEILER & HÄUSER 1997) because he found both taxa sympatric at Kendevan pass and treats pseudoxerxes as a subspecies of altivagans. He established a chromosome number of n=16 for pseudoxerxes from Kendevan Pass. The chromosome number of populations from East Elburs was 22 New karyological results in Agrodiaetus unknown until now. We were able to determine a haploid chromosome number of n=15 in a specimen from the type locality Schakuh. dizinensis (Schurian, 1982) The karyotype of this species was unknown until now. We found a haploid chromosome number of n=17 in a specimen from the type locality Dizin (Tehran, Iran), the only known locality of this species. cyaneus (Staudinger, 1899) Variable chromosome numbers of n=16-20 were found in different populations from Iran, Azarbaijan, Armenia and Turkey (Van Province) (DE LESSE 1960a, 1963b; LUKHTANOV et al. 1998; LUKHTANOV 1989; LUKHTANOV & DANTCHENKO (2002a)). Our results from Van Province (n=18) and Kars Province (n=17) fall within this range. Several subspecies of cyaneus have been described from Iran which are sometimes treated as distinct species (ssp. xerxes (Staudinger, 1899) from Elburs Mts., ssp. paracyaneus (De Lesse, 1963) from Hamadan, ssp. damalis (Riley, 1921) from Karind Gorge (Zagros Mts.), ssp. fredi Eckweiler, 1997 from Fars and ssp. kermansis (De Lesse, 1962) from Kerman). merhaba De Prins, van der Poorten, Borie, Oorschot, Riemis & Coenen, 1991 This species is only known from its type locality at Yusufeli (Artvin Province). Its chromosome number is n=16-17 (LUKHTANOV et al. 1998) and this figure can be confirmed by our data. zarathustra Eckweiler, 1997 The karyotype of this species from Dorud (Lorestan, Iran) was unknown until now. According to our results, it has a haploid chromosome number of n=ca 22. actis (Herrich-Schäffer, [1851]) OLIVIER (2000a) discovered the syntypes of this species and designated a lectotype from Tokat. This was important because the type locality of this taxon was very imprecisely stated as “Kleinasien” (= Asia minor) in its original description. Several recent authors including HESSELBARTH et al. (1995) and ECKWEILER & HÄUSER (1997) treated a different taxon under the name actis (see under sigberti). The chromosome number of actis from the type locality or surroundings is unknown and the expedition to Central Turkey in 1998 did not succeed in finding this species near Tokat or Amasya. The closest population we found was near Gürün (Sivas Province) of which we established a haploid chromosome number of n=17 from two specimens. This came as a surprise because only much higher chromosome numbers (n=2434) are known from phenotypically similar populations in other parts of Turkey, all of them more distant from Tokat. These populations are provisionally treated under the following name: firdussii (Forster, 1956) An extraordinary variation of chromosome numbers (n=21-34) has been recorded for this taxon and several authors suggested that it consists of different species, but there is no clear geographical pattern and populations with different karyotypes have no distinctive phenotypic features. The chromosome number of this species from the type locality Schakuh (East Elburs Mts., Iran) is unknown, but DE LESSE (1960a, 1962a) obtained counts of relatively high numbers (n=31-34) from Demavend (East Elburs). He recorded similar chromosome numbers also from Kop Dağı in Turkey (n=30-32), but in another location just 80km to the west (25 km N Erzincan) he obtained chromosome numbers of only n=24-25. This material from Erzincan was included in the type series of Agrodiaetus bilgini Dantchenko & Lukhtanov, 23 Chapter 2 2002. The description of this taxon, with the holotype from Torul (Gümüşhane Prov., Turkey) was based only on the difference in chromosome numbers (n=25). We checked a population 25 km SE Erzincan (Çağlayan) and obtained chromosome counts of n=30-32 in three specimens. LUKHTANOV et al. (1998) found low chromosome numbers also in Van Province (n=21-23 at Güzeldere Geçidi and n=25 at Çatak) and Bitlis Province (n=25 at Kuzgunkiran Geçidi). The material of the latter two populations was included in the type series of Agrodiaetus haigi Dantchenko & Lukhtanov, 2000, together with the holotype from Çatak (n=25) and further paratypes from Çatak (n=25-26), Kurubas Geçidi (n=25) and Güzeldere Geçidi (n=25). Our chromosome counts from Van Province (n=25 from Çatak and n=26 from Güzeldere Geçidi) confirm these results. The picture is complicated by the fact that intermediate chromosome numbers of n=27-29 are also known from populations in northwest Iran and eastern Turkey. DE LESSE (1960a, 1962a) found a haploid chromosome number of n=28 near Tabriz (Azarbayjan-e Sharqi, Iran) and n=27-29 at Mirgemir Dağı (Ağrı, Turkey). We also found a population with n=28-29 near Zanjan (Iran) and with n=25-27 in Dez valley (Hakkari Prov., Turkey). pseudactis (Forster, 1960) This taxon which was described from Daralagez Mts. in Nachichevan (Azerbaydzhan) is very similar to firdussii and its status (species, subspecies or synonym) is in dispute. LUKHTANOV & DANTCHENKO (2002a) obtained a chromosome number of n=29 from Armenian populations. artvinensis (Carbonell, 1997) Another species which is closely related to firdussii and only known from Erzurum Prov. (type locality: Tortum) and Artvin Prov. in Turkey. Its chromosome number was found to be n=21-22 at Kilizkaya in Artvin Prov. (OLIVIER et al. 2000) and we can confirm these results from the same locality. sigberti Olivier, van der Poorten, Puplesiene & De Prins, 2000 This name was introduced to denote a taxon previously referred to under the name actis. It is mainly distributed in the Taurus Mts. and a few locations in the Pontic chain. The karyotype was described by LUKHTANOV & DANTCHENKO (2002b) who counted n=29 chromosomes in two paratypes from the type locality (Aladaglari, Niğde Prov., Turkey). We checked males from the same locality and obtained n=25 and n=28 in two different specimens. DE LESSE (1962a) found n=27 in a population from Bürücek (İçel Prov.) which probably belongs to this taxon. ernesti Eckweiler, 1989 This taxon which was described as a subspecies of firdussii was only known from the type locality (Kohu Dağı in Antalya Prov., Turkey) and its karyotype was unknown until now. We found two specimens at a new locality (Dedegöl Geçidi, Isparta Prov.) of which one revealed its karyotype which is n=18. sertavulensis (Koçak, 1979) The karyotype of this taxon described as a subspecies of pseudactis and only known from the Taurus mountains was unknown until now. We were able to establish its karyotype from two specimens from Yellibelli Geçidi (Karaman Prov.) which is 33 km West of the type locality (Sertavul Pass) as n=20. 24 New karyological results in Agrodiaetus maraschi (Forster, 1956) This taxon has been described from Kahramanmaraş as a subspecies of damone and treated as a distinct species (KOÇAK 1979), as a synonym of wagneri (s. HESSELBARTH et al. 1995) or as a subspecies of firdussii (s. ECKWEILER & HÄUSER 1997). DE LESSE (1962a) found a specimen of this taxon at Erciyes Dağı (Kayseri) and established a haploid chromosome number of n=16. We can confirm this result from a specimen found near Gürün which is 40 km closer to the type locality. altivagans (Forster, 1956) This taxon was described from the high altitudes (2800m) of Daralagez Mts. (Nachichevan, Azerbaydzhan). DE LESSE (1962a) records a variable chromosome number from Turkish populations: n=21-23 in Erzurum and Ağrı Prov., but a lower number of n=18 in two populations near Erzincan. LUKHTANOV & DANTCHENKO (2002a) confirmed the first figure for two Armenian populations (n=20-23) and we can confirm it for Güzeldere Geçidi in Van Province of Turkey (n=21-23). DE LESSE (1962a) also checked Iranian populations which he referred to as “A. altivagans ssp.” from Marand (n=19-22), Tabriz (n=18), Hamadan (n=18) and Sanandadj (n=17-18). The latter two were described as ssp. ectabanensis (de Lesse, 1963) while the first two were left undescribed due to lack of sufficient material. The one from Marand probably belongs to the following taxon: gorbunovi Dantchenko & Lukhtanov, 1994 This taxon was described as a new species which is closely related to ectabanensis from Talysh Mts. in Azerbaydzhan. Its chromosome number has not been reported, but the specimens of “altivagans ssp.” which DE LESSE (1962a) caught east of Marand (n=19-22) might represent this taxon. We collected two specimens at Dugijan (30 km East of Marand) and obtained a chromosome number of n=ca. 20. Another specimen from Ahar Pass (Azarbayjan-e Sharqi) gave the same result and a specimen from Khalkhal, Gollijeh (Ardabil) had n=19 chromosomes. wagneri (Forster, 1956) This taxon was described as a subspecies of damone and elevated to species rank by HESSELBARTH et al. (1995). DE LESSE (1960a, 1962a) karyotyped a specimen from the type locality Aksehir (Konya Prov., Turkey) and found n=16 chromosomes. Other results have not been published, yet. We found a specimen at Zelve (Nevşehir) which resembles the holotype but has n=18 chromosomes. turcicus (Koçak, 1977) The karyotype of this Eastern Anatolian species has been described by LUKHTANOV et al. (1998) of populations from Erzurum and Çatak (Van Prov.). It is one of only two species of Agrodiaetus known to have a stable haploid chromosome number of n=24 which is the modal chromosome number in Lycaenidae. (The other species is poseidonides (Staudinger, 1886)). We can confirm this result from Çağlayan (Erzincan Prov.). baytopi (de Lesse, 1959) DE LESSE (1959d) established the karyotype of this Eastern Anatolian species in his original description as n=27 from the type locality (Doğubayazıt, Ağrı Prov.). We can confirm this result from Güzeldere Geçidi (Van Prov.). DE LESSE (1959d) had problems to find eupyrene spermatogonia in this species and we also managed to find a good metaphase in only one out of 13 investigated specimens. 25 Chapter 2 rovshani Dantchenko & Lukhtanov, 1994 The karyotype of this species which has been described from Talysh mts. in Azerbaidzhan and which seems to be closely related to baytopi remains unknown. We did not obtain a result from a specimen collected in Dugijan (Azarbayjan-e Sharqi, Iran). tankeri (de Lesse, 1960) DE LESSE (1960c) described this species after he discovered (DE LESSE 1957, 1959e) that populations externally similar to iphigenia from Kop Dağı Geçidi and Mirgemir Dağı have a different karyotype with n=20-21 chromosomes and both taxa occur sympatrically on Mirgemir Dağı. The karyotype is asymmetric with nine chromosomes much larger than the other ones. We checked nine specimens from the type locality (Kopdağı Geçidi) and one from Gölyurt Geçidi (Erzurum) but were unable to find any eupyrene metaphases. iphigenia (Herrich-Schäffer, [1847]) A variable chromosome number of n=12-16 has been found in Anatolian populations. The lowest numbers (n=12) were found in NE Turkey, n=13 mainly in SE Turkey and n=15-16 in Eastern Taurus (DE LESSE 1959e; LUKHTANOV et al. 1998). We can confirm n=12 for Erzincan and found n=14-15 in Western Taurus (Fethiye and Isparta Prov.). The ssp. nonacriensis (Brown, 1977) has been described from Peleponnesos, Greece, at the Western distributional limit of this species. The separation was made partly due to its lower chromosome numbers of n=10-13 (BROWN 1977), but most other chromosome counts of this author have turned out to be erroneous, thus it should be verified again. iphicarmon Eckweiler & Rose, 1993 This taxon was described as a subspecies of iphigenia although it was found sympatrically with the latter taxon at the type locality Dedegöl Geçidi (Isparta Prov., Turkey), its only known locality (ECKWEILER & ROSE 1993). We revealed its karyotype which has a chromosome number of n=29. We were also able to check a specimen of iphigenia from the same locality which turned out to have n=15. This proves that iphicarmon cannot be conspecific with iphigenia. iphidamon (Staudinger, 1899) This North Iranian taxon is often treated as a subspecies of iphigenia (e.g. HESSELBARTH et al. 1995) because of its similar chromosome number (n=14 at Āb Ali and Firuzkuh in Eastern Elburs according to DE LESSE 1959e). We can confirm this number now for the type locality Schakuh and for Hajiabad (Golestan, Iran). damon ([Denis & Schiffermüller], 1775) The most widespread Agrodiaetus species which is found from Northern Spain to Mongolia seems to have a stable chromosome number of n=45 (with one large chromosome) throughout its range. Counts have been made in populations from the French Pyrenees, French Alps, Mt. Ararat (Turkey) and Altai Mts. (DE LESSE 1960a; LUKHTANOV 1989). We did not obtain cells with typical divisions from our four preparations from Eastern Turkey. mofidii (de Lesse, 1963) DE LESSE (1963a) described this species from Kopet Dağı, Iran with a chromosome number of n=34-35 (n=34 in the holotype). We checked two specimens from Kuh-e-Sorkh (Khorasan) of the newly described ssp. sorkhensis Eckweiler, 2003. These turned out to have a higher chromosome number of n=ca.42-45 and might therefore represent a different species. 26 New karyological results in Agrodiaetus phyllis (Christoph, 1877) DE LESSE (1959b) studied material from Kendevan Pass and Demavend (Elburs Mts., Iran) and found only a slight variation in chromosome numbers (n=79-82) and a very peculiar karyotype with one extremely large and three other large chromosomes while the rest was very small. A very similar karyotype with n=78 was found by DE LESSE (1957, 1959b) in several Eastern Anatolian populations (ssp. vanensis (de Lesse, 1957)) and by LUKHTANOV & DANTCHENKO (2002a) with n=79-80 in Armenia (ssp. sheljuzhkoi (Forster, 1960)) while the karyotype of dagestanicus (Forster, 1960) from Dagestan (Russia) turned out to be very different (n=40) and represents another species (LUKHTANOV & DANTCHENKO 2002a). We are able to confirm this karyotype not only from Kendevan Pass and Demavend but also from Schakuh, the type locality of phyllis. We found n>=82 chromosomes in specimen MW00348 and can confirm the existence of one huge outstanding bivalent, which is 10 times larger than the mid-size bivalents as well as three large bivalents which are 3-4 times larger than the midsize bivalents. posthumus (Christoph, 1877) DE LESSE (1957, 1959b) checked the karyotype of specimens which he thought to represent this taxon and found their karyotype to be very different from phyllis with n=10-11 chromosomes. However he did not check topotypical material from Schakuh, but from other localities in Elburs Mts. (Kendevan Pass, Demavend, Firuzkuh) and these populations belong to a different taxon later named birunii (see the next paragraph). We were first to examine material from the type locality of posthumus and found a peculiar karyotype totally different from birunii with a high number of n=ca 85 chromosomes, two of them about 15 and a third about 10 times bigger than the remaining chromosomes. The karyotype of phyllis which occurs sympatrically at Schakuh has a very similar number of chromosomes but a very different karyotype (see previous paragraph). Both species are easy to identify at Schakuh whereas birunii and phyllis can easily be confused with each other (DE LESSE 1959b: 9). birunii Eckweiler & ten Hagen, 1998 This taxon was described as a subspecies of posthumus from Firuzkuh, East Elburs, but due to the totally different karyotype it must be regarded as specifically distinct from posthumus as well as from phyllis. A specimen from the type locality was karyotyped by DE LESSE (1959b) who found a haploid chromosome number of n=10 which is the lowest chromosome number known in Agrodiaetus. Further material of this taxon was karyotyped by DE LESSE (1959b) from Demavend and Kendevan Pass. We are able to confirm a chromosome number of n=1011 (or 2n=20-22) from Veresk (near the type locality), from Polur (Demavend), from three populations near Kendevan Pass (Pul-e Zanguleh, Gardaneh-ye Lavashm, Golestanak Nature Reserve) as well as from two new localities: Dizin Pass and Takht-e Suleyman. darius Eckweiler & ten Hagen, 1998 This taxon from Elburs Mts. (Iran) was described as a new species because it was found sympatrically with birunii which was regarded as a subspecies of posthumus by ECKWEILER & TEN HAGEN (1998). However, birunii is specifically distinct from posthumus (see previous paragraph) and thus it cannot be ruled out that darius is a subspecies of posthumus. Both taxa are allopatric and have a similar phenotype. Unfortunately the karyotype of darius remains unknown. We checked a specimen from the type locality (Polur) and from Dizin, but were unable to find typical meiotic divisions. Recently a population attributed to darius was discovered in the Iranian Talysh Mts. (Azerbaijan-e Sharqi) near Masuleh and described as a new subspecies (masulensis ten Hagen & Schurian, 2000). 27 Chapter 2 iphigenides-group iphigenides (Staudinger, 1886) The chromosome number of this Central Asian species was found to be n=65-67 in different populations from Kirgizia and Uzbekistan (LUKHTANOV & DANTCHENKO 2002a). No chromosome fixations were available to us. poseidonides (Staudinger, 1886) The chromosome number of this Central Asian species was found to be n=24 in two populations from Kirgizia (LUKHTANOV & DANTCHENKO 2002a). It is one of only two species of Agrodiaetus known to have a stable haploid chromosome number of n=24 which is the modal chromosome number in Lycaenidae. (The other species is turcicus (Koçak, 1977). No chromosome fixations were available to us. dagmara-group dagmara (Grum-Grshimailo, 1888) The karyotype of this Central Asian species is unknown. No chromosome fixations were available to us. erschoffii-group glaucias (Lederer, 1871) The karyotype of this species which has been described from Hajiabad and Schakuh in Elburs Mts. of Iran remains unknown. We did not manage to find this species on our visit at the type localities. erschoffii (Lederer, 1869) DE LESSE (1963a) and LUKHTANOV & DANTCHENKO (2002a) recorded a chromosome number of n=13-15 from Kopetdag (Turkmenia) from where ssp. tekkeanus (Christoph, 1887) was described. We can confirm this chromosome number for nominotypical erschoffii from the type locality Hajiabad (East Elburs Mts., Iran). unknown group The following taxa have been described after 1997 and are not apparently closely related to any of the taxa listed by ECKWEILER & HÄUSER (1997): klausschuriani ten Hagen, 1999 The chromosome number of this newly discovered species was not known until now. We checked six specimens from the type locality Veresk in eastern Elburs Mts. and found a chromosome number of n=56 in three of them. tenhageni Schurian & Eckweiler, 1999 Two chromosome preparations of this newly discovered taxon from Khorasan (Iran) did not reveal typical divisions and therefore the karyotype remains unknown. SCHURIAN & ECKWEILER (1999) could not place it into any group. arasbarani (Carbonell & Naderi, 2000) The chromosome number of this new taxon from Arasbaran (Azarbaijan-e-Sharqi, Iran) remains unknown. 28 New karyological results in Agrodiaetus shahrami Skala, 2001 The chromosome number of this endemic from high altitudes of Zagros Mts. (Zarde-Kuh, 3000-3300m, Bakhtiari, Iran) was determined by V. Lukhtanov (SKALA 2002b) from one preparation with meiotic divisions as n=128-131, which constitutes the highest chromosome number so far known in Agrodiaetus. achaemenes Skala, 2002 Only atypical divisions with a high number of chromosomes were found in two specimens of this taxon which was recently discovered by SKALA (2002b) at very high altitudes (38004000m) in the Iranian Zagros range (Kuh-e-Haft, Bakhtiari). paulae Wiemers & De Prins, 2003 At Ahar Pass (Azarbayjan-e Sharqi, Iran) a new taxon of Agrodiaetus was found (WIEMERS & DE PRINS, in print) whose phenotype has similarities to elbursicus (wing colour of the upperside) and altivagans (reduced wing markings on the underside). Its chromosome number was determined as n=17 from six specimens. At Ahar Pass this taxon was flying together with gorbunovi, which is thought to be closely related to altivagans and has n=20 chromosomes. Tab. 2 presents a synoptic alphabetical list of the above mentionned taxa with chromosome counts. It also includes further Agrodiaetus species of which chromosome numbers are known, with corresponding references. Discussion The correct establishment of the karyotype is a very difficult task, especially in the many Agrodiaetus species with high chromosome numbers and very small chromosomes. The only possibility to count them is in the Metaphase I of Meiosis when the bivalents are in a condensed form and well separated from each other. Even then, their size can be below 1µm which is at the absolut limit of resolution for light microscopy.Irregular (apyrene) spermatogenesis (FRIEDLÄNDER 1997) is the dominant form in adult Agrodiaetus, but correct chromosome counts can usually only be made from eupyrene spermatogonia which are much less common. Difficulties in finding “good” metaphase cells with non-overlapping bivalents can lead to erroneous counts. The presence of multivalents and macrochromosomes which can be confused with each other or possibly supernumerary chromosomes further complicates the task (LORKOVIĆ 1990). Not surprisingly, erroneous results have also been published, one unfortunate example being BROWN (1976b) and BROWN & COUTSIS (1978) with totally wrong chromosome counts (COUTSIS et al. (1999); DE PRINS, pers. comm.). Therefore caution should be applied if chromosome counts are ambiguous or cells are of poor quality. Nevertheless, a large part of the variation found in Agrodiaetus species is due to methodical difficulties, especially in the case of the squash techniques which often result in overlapping bivalents which can lead to an underestimate of chromosome numbers. Only recently, a new two-phase method of chromosome preparation was developed (LUKHTANOV & DANTCHENKO 2002a) which overcomes some of these problems, because the original position of chromosomes is less distorted. The disadvantage of this method is that no durable preparations can be made and photographs remain the only evidence. This method was applied in a part of the preparations (since 2002) and often yielded more precise chromosome counts. Despite the methodological difficulties there is some evidence that slight variation of bivalent chromosome numbers (not involving supernumerary chromosomes) within a population and even within individuals may exist. We observed such variation e.g. in A. birunii (n=10-11), A. iphigenia (n=12-14) and A. poseidon (n=19-21). More studies are needed to understand this phenomenon. More common is slight variation of chromosome numbers between populations and it is not known whether these differences are effective as genetic barriers to gene flow. 29 Chapter 2 Tab. 2. Chromosome numbers in Agrodiaetus 32-35 18 13-15 90 27 24-32 103 66 ? 19-20 42 Own results (haploid chromosome numbers) according to Provinces (E=Spain, I=Italy, TR=Turkey, IR=Iran) or references see text TR: Sivas (17) TR: Antalya (80), Adana (ca78-80) E: Alava (ca108), Huesca (ca108) IR: Zanjan (19), TR: Hakkari (19), Van (19), Karaman (20), Adana (21) TR: Van (ca21-23), Erzincan (ca21) TR: Hakkari (42) see text DE LESSE (1961b) see text TR: Artvin (ca21-22) LUKHTANOV & DANTCHENKO (2002a) TR: Van (ca27-28) LUKHTANOV & DANTCHENKO (2002b) IR: Tehran (10-11), Mazandaran (10-11) IR: Golestan (20) TR: Artvin (ca80) LUKHTANOV (1989) LUKHTANOV & DANTCHENKO (2002a) TR: Kars (17), Van (18-19) LUKHTANOV & DANTCHENKO (2002a) see text see text see text see text LUKHTANOV et al. (1997) TR: Van (ca40-42) see text LARSEN (1974b) IR: Tehran (ca70), Azarbaijan (ca70-80); TR: Kars (ca66), Hakkari (ca60-70) IR: Tehran (17) DE LESSE (1962b, 1966) DE LESSE (1964) IR: Tehran (17-18), Mazandaran (16-18), Zanjan (18) LUKHTANOV & DANTCHENKO (2002a) TR: Isparta (18) IR: Golestan (ca13-14) E: Soria (>75) IR: Zanjan (27), Ardabil (27) IR: Zanjan (28-29); TR: Erzincan (ca30-32) E: Tarragona (ca98-103) TROIANO & GIRIBALDI (1979) see text IR: Azarbaijan (20), Ardabil (19) see text 21-25 21-22 15 33-37 38 29-32 29 TR: Van (24-26), Hakkari (25-27) IR: Tehran (21-22) TR: Malatya (ca15-16), Van (15), Hakkari (15) TR: Artvin (36), Kars (ca33-34), Bayburt (ca33) I: Aosta (ca38) TR: Erzincan (31) TR: Isparta (29) achaemenes Skala, 2002 actis (Herrich-Schäffer, [1851]) admetus (Esper, [1783]) ainsae (Forster, 1961) alcestis (Zerny, 1932) n (new= bold) ? 17 78-80 108-110 19-21 altivagans (Forster, 1956) antidolus (Rebel, 1901) arasbarani (Carbonell & Naderi, 2000) ardschira (Brandt, 1938) aroaniensis (Brown, 1976) artvinensis (Carbonell, 1997) aserbeidschanus (Forster, 1956) baytopi (de Lesse, 1959) bilgini (Dantchenko & Lukhtanov, 2002) birunii Eckweiler & ten Hagen, 1998 caeruleus (Staudinger, 1871) carmon (Herrich-Schäffer, [1851]) carmonides Eckweiler, 1997 ciscaucasicus Forster, 1956 cyaneus (Staudinger, 1899) dagestanicus Forster, 1960 dagmara (Grum-Grshimailo, 1888) dama (Staudinger, 1892) damocles (Herrich-Schäffer, [1844]) damon ([Denis & Schiffermüller], 1775) damone (Eversmann, 1841) dantchenkoi (Lukhtanov & Wiemers, 2003) darius Eckweiler & ten Hagen, 1998 deebi (Larsen, 1974) demavendi (Pfeiffer, 1938) 18-23 40-42 ? 113-115 48 21-22 22-23 27 25 10-11 20 81-82 15-16 16 16-20 40 ? 41 23-26 45 66-68 40-42 ? 17 66-76 dizinensis (Schurian, 1982) dolus (Hübner, [1823]) ectabanensis (De Lesse, 1963) elbursicus (Forster, 1956) 17 123-125 18 16-18 Agrodiaetus-Taxa eriwanensis (Forster, 1960) ernesti Eckweiler, 1989 erschoffii (Lederer, 1869) fabressei (Oberthür, 1910) femininoides (Eckweiler, 1987) firdussii (Forster, 1956) fulgens (de Sagarra, 1925) galloi (Baletto & Toso, 1979) glaucias (Lederer, 1871) gorbunovi Dantchenko & Lukhtanov, 1994 guezelmavi Olivier, Puplesiene, van der Poorten, De Prins & Wiemers, 1999 haigi (Lukhtanov & Dantchenko, 2002) hamadanensis (de Lesse, 1959) hopfferi (Herrich-Schäffer, [1851]) huberti (Carbonell, 1993) humedasae (Toso & Baletto, 1976) interjectus (de Lesse, 1960) iphicarmon Eckweiler & Rose, 1993 30 New karyological results in Agrodiaetus Agrodiaetus-Taxa iphidamon (Staudinger, 1899) iphigenia (Herrich-Schäffer, [1847]) iphigenides (Staudinger, 1886) juldusus (Staudinger, 1886) kanduli (Lukhtanov & Dantchenko, 2002) karindus (Riley, 1921) khorasanensis (Carbonell, 2001) klausschuriani ten Hagen, 1999 kurdistanicus (Forster, 1961) lorestanus Eckweiler, 1997 lycius (Carbonell, 1996) maraschi (Forster, 1956) menalcas (Freyer, [1837]) merhaba De Prins, van der Poorten, Borie, Oorschot, Riemis & Coenen, 1991 mithridates (Staudinger, 1878) mofidii (de Lesse, 1963) morgani (Le Cerf, 1909) nephohiptamenos (Brown & Coutsis, 1978) ninae (Forster, 1956) paulae Wiemers & De Prins, 2003 peilei Bethune-Baker, 1921 pfeifferi (Brandt, 1938) phyllides (Staudinger, 1886) phyllis (Christoph, 1877) pierceae (Lukhtanov & Dantchenko, 2002) poseidon (Herrich-Schäffer, [1851]) poseidonides (Staudinger, 1886) posthumus (Christoph, 1877) pseudactis (Forster, 1960) pseudoxerxes (Forster, 1956) putnami (Lukhtanov & Dantchenko, 2002) ripartii (Freyer, 1830) rovshani Dantchenko & Lukhtanov, 1994 schamil (Dantchenko, 2000) schuriani (Rose, 1978) sekercioglui (Lukhtanov & Dantchenko, 2002) sennanensis (de Lesse, 1959) sertavulensis (Koçak, 1979) shahrami Skala, 2001 sigberti Olivier, van der Poorten, Puplesiene & De Prins, 2000 sorkhensis Eckweiler, 2003 surakovi Dantchenko & Lukhtanov, 1994 tankeri (de Lesse, 1960) tenhageni Schurian & Eckweiler, 1999 theresiae Schurian, van Oorschot & van den Brink, 1992 transcaspicus (Heyne, [1895]) turcicola (Koçak, 1977) turcicus (Koçak, 1977) valiabadi (Rose & Schurian, 1977) wagneri (Forster, 1956) zapvadi (Carbonell, 1993) zarathustra Eckweiler, 1997 n (new= bold) 14 12-16 65-67 67 25 68 84 56 57-62 69-74 21-22 16 85 16-17 21-27 34-35 25-27 ca.90 33-37 17 39 106-108 ca. 66 78-82 21-22 19-22 24 ca. 85 29 15-16 25-27 90 ? 17 81-82 50 28-30 20 128-131 25-29 Own results (haploid chromosome numbers) according to Provinces (E=Spain, I=Italy, TR=Turkey, IR=Iran) or references IR: Mazandaran (14), Golestan (14) TR: Kars (ca11-14), Erzincan (12), Fethiye (14), Isparta (15) LUKHTANOV & DANTCHENKO (2002a) LUKHTANOV & DANTCHENKO (2002a) TR: Van (25) IR: Kordestan (68) see text IR: Mazandaran (56) TR: Van (56-62) see text TR: Antalya (21-22) TR: Sivas (16) TR: Fethiye (85), Antalya (>75), Van (85) TR: Artvin (ca17) TR: Malatya (23) see text IR: Kordestan (27) see text TR: Ağrı (34) IR: Azarbaijan (17) IR: Kordestan (39) DE LESSE (1961b) LUKHTANOV & DANTCHENKO (2002a) IR: Tehran (ca76-78), Golestan (ca82-86) TR: Van (21) TR: Nevşehir (21-22), Sivas (19) see text IR: Golestan (ca85) see text IR: Golestan (15) TR: Ağrı (25) E: Burgos (ca90); TR: Isparta (>80), Adana (ca90), Artvin (ca90), Erzincan (>85), Van (>71) see text LUKHTANOV & DANTCHENKO (2002a) TR: Kayseri (ca75-80) TR: Van (>45) see text TR: Karaman (20) see text TR: Kayseri (25,28) ca.45 50 20-21 ? 63 IR: Khorasan (ca45) see text see text see text TR: Adana (59-63) 52-53 19-22 24 23 16-18 18-19 ca. 22 see text TR: Van (19-22) TR: Iğdır (ca25), Erzincan (ca24) IR: Mazandaran (23) TR: Nevşehir (18, 19-21?) TR: Van (18-19) IR: Lorestan (ca22) 31 Chapter 3 32 A molecular phylogeny of Agrodiaetus Chapter 3: A molecular phylogeny of Agrodiaetus inferred from mitochondrial and nuclear DNA sequences Material and methods Material Material for this study was collected on trips to Turkey, Iran, Italy, France, Spain and Morocco during the years 1998-2002. While testes or abdomens were removed for karyological analysis and wings cut off for wing pattern analysis, the remaining body was placed in vials with 100% ethanol and later stored at -20°C. The parts of each specimen were coded “MW” and numbered individually. Further material was received from the following collegues: Code AD CN DS JC JM OK WE Name Alexandre Dantchenko (Moscow) Clas Naumann (Bonn) Dmitriy Sobanin (Voronezh) John Coutsis (Athens) José Munguira (Madrid) Otakar Kudrna (Schweinfurt) Wolfgang Eckweiler (Frankfurt) Countries Armenia Turkey, Iran Central Asia Greece Spain Italy, Spain mainly Iran Storage of material Ethanol Ethanol dried Ethanol; wings separated Ethanol; wings separated frozen dried; abdomens & wings separated A complete list of available material is found in Appendix 2. Tab. 3. Material used for DNA extraction Specimens Genus Agriades Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Spezies pyrenaicus achaemenes actinides actis admetus ainsae alcestis altivagans antidolus arasbarani aroaniensis artvinensis barmifiruze baytopi birunii caeruleus carmon cyaneus dagmara dama DNA COI MO E F I 1 1 1 1 2 0 3 1 3 2 3 3 3 7 5 5 5 3 3 1 1 1 1 1 1 1 0 3 3 7 7 2 2 3 2 4 4 1 1 1 1 Populations (COI) Specimens GR TR AR IR UZ TA KI 1 1 1 1 1 4 2 3 1 1 1 1 1 2 6 2 2 2 1 1 1 ∑ ND1 Cytb ITS2 1 0 0 1 1 0 0 1 0 0 0 1 1 0 0 1 2 0 1 1 3 0 0 2 5 1 0 3 3 0 0 3 3 1 0 2 1 0 0 1 1 0 0 1 1 0 0 1 1 0 0 0 2 0 0 2 6 1 0 5 2 1 0 1 2 0 0 1 3 0 0 3 1 0 0 0 1 0 0 1 33 Chapter 3 Specimens Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus 34 Spezies damalis damocles damon damone dantchenkoi darius demavendi dizinensis elbursicus ernesti erschoffii fabressei femininoides firdussii fulgens glaucias gorbunovi guezelmavi hamadanensis hopfferi huberti humedasae interjectus iphicarmon iphidamon iphigenia iphigenides kanduli karindus khorasanensis klausschuriani kurdistanicus lorestanus lycius menalcas merhaba mithridates mofidii morgani nephohiptamenos ninae paulae peilei phyllides phyllis pierceae poseidon poseidonides posthumus pseudactis pseudoxerxes putnami Populations (COI) DNA COI MO E F I GR TR AR IR UZ TA KI 1 1 1 1 0 2 2 1 1 2 0 4 4 2 1 1 1 12 12 2 3 1 1 1 7 7 6 1 1 1 2 2 2 2 2 2 2 2 2 7 7 3 2 1 1 1 1 1 1 4 4 3 2 1 1 3 3 3 5 2 2 4 4 3 1 3 2 1 1 1 1 2 1 1 3 3 3 6 5 3 1 2 2 1 1 1 1 1 1 1 1 1 1 1 2 2 1 2 2 2 2 1 1 3 2 2 5 3 3 2 2 1 2 1 1 2 1 1 1 1 1 2 2 1 2 2 1 1 2 2 1 1 1 1 3 0 7 6 2 1 3 3 2 2 6 4 2 1 1 1 2 2 1 1 1 1 2 2 2 2 2 2 Specimens ∑ ND1 Cytb ITS2 1 0 0 0 0 0 0 0 2 0 0 2 0 0 0 0 2 0 0 3 1 0 0 1 5 2 0 6 1 0 0 1 6 1 0 5 1 0 0 1 2 0 0 1 2 0 0 2 2 0 0 2 5 0 0 5 1 0 0 1 1 0 0 1 3 0 0 4 1 0 0 1 3 1 0 1 2 0 0 2 4 0 0 3 1 0 0 2 1 1 0 1 1 0 1 1 3 1 0 2 4 0 0 2 2 0 0 1 1 0 0 1 1 0 0 0 1 0 0 1 1 0 0 1 2 0 0 1 1 0 0 2 2 0 0 1 3 1 0 1 1 0 0 1 1 0 0 1 1 0 0 1 1 0 0 1 1 0 0 1 2 0 0 2 1 0 0 1 1 0 0 1 0 0 0 0 6 0 0 4 2 0 0 2 2 1 0 3 1 0 0 1 1 0 0 1 1 0 0 1 2 0 0 1 2 0 0 1 A molecular phylogeny of Agrodiaetus Specimens Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Aricia Aricia Aricia Aricia Aricia Aricia Aricia Cacyreus Celastrina Chilades Cupido Cyaniris Favonius Iphiclides Iphiclides Kretania Lampides Leptotes Lycaena Lycaena Lycaena Lycaena Lycaena Lycaena Lycaena Lycaena Lysandra Lysandra Lysandra Lysandra Lysandra Lysandra Lysandra Spezies ripartii rovshani schuriani sekercioglui sennanensis sertavulensis shahrami sigberti surakovi tankeri tenhageni theresiae transcaspicus turcicola turcicus valiabadi wagneri zapvadi zarathustra agestis anteros artaxerxes cramera eumedon isauricus torulensis marshalli argiolus trochylus osiris semiargus quercus feisthamelii podalirius eurypilus boeticus pirithous alciphron asabinus candens phlaeas thersamon thetis tityrus virgaureae albicans bellargus caelestissimus coridon corydonius gennargenti ossmar Populations (COI) DNA COI MO E F I GR TR AR IR UZ TA KI 10 9 3 1 4 2 2 2 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 3 2 1 1 1 1 2 2 1 2 1 1 1 1 1 1 0 3 2 2 3 3 3 2 2 2 4 3 2 3 3 2 1 1 1 4 4 1 1 1 1 1 1 1 3 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 1 1 1 1 1 1 1 1 2 2 1 1 1 1 1 2 2 1 1 1 1 1 1 1 1 3 3 1 1 1 1 1 1 2 2 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 2 2 2 6 5 1 1 2 1 1 1 1 5 5 4 1 5 4 4 1 1 1 2 2 2 Specimens ∑ ND1 Cytb ITS2 8 1 0 4 2 0 0 2 1 0 0 1 1 0 0 0 1 0 0 1 1 0 0 1 1 0 0 1 1 0 0 1 1 0 0 1 1 0 0 2 1 0 0 1 1 0 0 1 0 0 0 0 2 0 1 1 3 1 0 2 2 0 0 2 2 0 0 3 2 0 0 2 1 0 0 1 4 0 0 1 1 0 0 1 2 1 0 2 1 0 0 1 1 0 0 1 1 0 0 1 1 0 0 1 1 0 0 1 2 0 0 1 1 0 0 0 1 0 0 0 2 0 0 2 1 1 0 1 2 0 0 0 1 0 0 1 1 0 0 1 3 0 0 3 1 0 0 0 2 0 0 0 1 0 0 0 1 0 0 0 1 0 0 0 1 0 1 0 1 0 0 0 1 0 0 0 1 0 0 0 2 0 0 2 5 0 0 4 1 0 0 1 5 0 0 4 4 0 0 2 1 0 0 0 2 0 0 2 35 Chapter 3 Specimens Populations (COI) Specimens DNA COI MO E F I GR TR AR IR UZ TA KI ∑ ND1 Cytb ITS2 Genus Spezies 1 1 1 1 1 0 1 Lysandra syriaca 1 1 1 1 1 0 0 Maculinea arion 6 3 2 1 3 1 0 2 Meleageria daphnis 2 2 2 2 0 0 1 Meleageria marcida 1 1 1 1 0 0 1 Neolysandra coelestina 1 1 1 1 0 0 1 Neolysandra corona 1 1 1 1 0 0 0 Neolysandra diana 2 2 1 1 0 0 1 Neolysandra fatima 1 1 1 1 0 0 1 Plebeius argus 1 1 1 1 0 0 0 Plebeius christophi 1 0 0 0 0 0 Plebeius cyane 3 1 1 1 0 0 1 Plebeius loewii 1 1 1 1 0 0 1 Plebeius pylaon 1 1 1 1 0 0 1 Polyommatus aedon 2 2 1 1 2 0 0 2 Polyommatus amandus 1 1 1 1 0 0 1 Polyommatus andronicus 5 3 3 3 0 1 3 Polyommatus cornelia 3 3 1 2 3 1 0 2 Polyommatus dorylas 2 2 1 1 2 1 0 2 Polyommatus eroides 2 1 1 1 0 0 0 Polyommatus eros 2 2 1 1 2 0 0 1 Polyommatus escheri 7 5 1 1 1 1 1 5 1 0 4 Polyommatus icarus 2 2 2 2 0 0 2 Polyommatus menelaos 3 3 2 2 1 0 2 Polyommatus myrrhinus 6 4 1 2 1 4 1 0 2 Polyommatus thersites 1 1 1 1 0 0 0 Pseudophilotes abencerragus 1 1 1 1 0 0 0 Pseudophilotes vicrama 1 1 1 1 0 0 0 Satyrium esculi 1 1 1 1 0 0 1 Satyrium hyrcanicum 2 1 1 1 0 0 0 Satyrium myrtale 1 1 1 1 0 0 1 Tarucus theophrastus 2 1 1 1 0 0 0 Turanana endymion 2 2 1 1 2 0 0 1 Vacciniina alcedo 1 1 1 1 0 0 1 Vacciniina morgianus 1 1 1 1 0 0 0 Zizeeria knysna Sum ∑ 370 312 12 27 2 4 13 126 8 81 1 2 1 277 24 5 209 • DNA = Number of specimens of which DNA was extracted (- = without result). • COI, ND1, Cytb, ITS2 = Number of specimens sequenced for these genes. • Populations (COI) = Number of populations sequenced for COI from Morocco (MO), Spain (E), France (F), Italy (I), Greece (GR), Turkey (TR), Armenia (AR), Iran (IR), Uzbekistan (UZ), Tajikistan (TA) and Kirgizia (KI) Nomenclature according to HESSELBARTH et al. (1995), TOLMAN & LEWINGTON (1997) & KUDRNA (2002) Selection of taxa Material used for DNA extraction is listed in Tab. 3, sorted alphabetically according to genus and species names. Altogether 370 specimens from 158 butterfly taxa were used for DNA extraction. These include 90 ingroup taxa (subgenus Agrodiaetus of genus Polyommatus) covering 63 of the 83 species rank taxa listed by ECKWEILER & HÄUSER (1997) from all species groups, and an additional 17 species which have been described since. Of the outgroup taxa 27 are closely related taxa of the genus Polyommatus (subgenera Cyaniris, Lysandra, Meleageria, Neolysandra & Polyommatus), 16 are from the same subtribe Polyommatiti (e.g. the genera/subgenera Agriades, Aricia, Chilades, Kretania, Plebeius, 36 A molecular phylogeny of Agrodiaetus Vacciniina), 11 from other subtribes within the tribe Polyommatini (Glaucopsychiti: Maculinea, Turanana; Scolitantiditi: Pseudophilotes; Celastriniti: Celastrina; Everiti: Cupido; Zizeeriti: Zizeeria; Taruciti: Tarucus; Leptotiti: Leptotes; Lampiditi: Lampides; Cacyreus), 12 from other tribes (Eumaeini: Satyrium; Theclini: Favonius; Lycaenini: Lycaena) of Lycaenidae and two taxa of another family of Papilionoidea (Papilionidae: Iphiclides). This selection covers all Polyommatus subgenera, all Polyommatini subtribes and all but one Lycaenidae tribus known to occur in Anatolia, the centre of Agrodiaetus radiation (nomenclature according to HESSELBARTH et al., 1995). DNA extraction In most cases parts of the thorax were cut into slices and subsequently used for DNA extraction, but in a few cases only dried legs were available and thus used. DNA was extracted with QIAGEN® DNeasy Tissue Kit according to the manufacturer’s protocol for mouse tail tissue. This method turned out to give the most consistent results and was the most economical compared to other methods tried out in the beginning: • phenol-chloroform extraction (time-consuming method using hazardous chemicals) • CTAB buffer based chloroform-isoamylalcohol extraction (time-consuming method yielding a high proportion of degraded DNA) • BIORAD® Quantum Prep® Aqua PureTM Genomic DNA Tissue Kit (fast saltprecipitation method prone to contamination with butterfly scales) Results of DNA extraction were checked with Agarose gel electrophoresis. Selection of genes Mitochondrial genes are especially well suited for phylogenetic studies of closely related species, because of the faster rate of evolution compared to nuclear genes (1-2 times faster in insects; HOY, 1994) and the shorter coalescence time due to their smaller effective population size (a quarter of an autosomal locus). Cytochrome Oxidase I was chosen because this gene has been used successfully in phylogenetic studies of closely related Lepidoptera species, including butterflies (BELTRAN et al. (2002); BROWER (1994); CATERINO & SPERLING (1999); MONTEIRO & PIERCE (2001); RAND et al. (2000); WAHLBERG & ZIMMERMANN (2000)). Initially experiments were also carried out with cytochrome b which has only rarely been used in Lepidoptera (but recent papers on butterflies were published by AAGARD et al. (2002) and TORRES et al. (2001)). Cytochrome Oxidase I is known to evolve relatively slowly compared to other mitochondrial genes, e.g. of the ND family. Therefore part of the mitochondrial ND1 gene was also sequenced for a selection of taxa. In Lepidoptera this gene has been used in phylogenetic studies of Nymphalidae (AUBERT et al., 1999; MARTIN et al., 2000) and Geometridae (ABRAHAM et al., 2001). Because “gene trees and species trees are not the same” (NICHOLS, 2001), conclusions drawn from a single locus can be misleading. This is especially true for mitochondrial DNA which is maternally inherited. Agrodiaetus species are thought to have evolved only recently and gene flow might still exist due to incomplete speciation and hybridization events. Hybridization can be detected if gene trees based on mtDNA and nuclear DNA are compared to each other. Therefore the internal transcribed spacer (ITS-2), a non-coding region between the nuclear ribosomal genes 5.8s and 28s was chosen for a comparative study. ITS-2 is a very variable region, while the flanking genes are very conservative, so that universal primers can be employed. ITS-2 has not been used in published phylogenetic studies of Lepidoptera yet, but was used successfully for studying the phylogeny of closely related species in other insect orders like Diptera, Hymenoptera, Coleoptera, Odonata (WEEKERS et al. 2001) and Blattodea. 37 Chapter 3 PCR and sequencing Amplification of DNA was conducted using the polymerase chain reaction (PCR). The reaction mixture (for a total reaction volume of 25µl) included: ddH20 16,80 µl 10xPCR II buffer (without MgCl2) 2,50 µl MgCl2 25mM 3,20 µl dNTP-Mix 2mM 0,50 µl Primer 1 20pm 0,375 µl Primer 2 20pm 0,375 µl Taq Polymerase 0,25 µl DNA 1,00 µl The primers used are shown in Tab. 4. Tab. 4. Primers Alias Designation* Sequence (5’ to 3’) References k698 Nancy Faw ND1 16sb cb1l cb2-h ITS-3 ITS-4 TY-J-1460 C1-N-2192 N1-J-12314 LR-N-12866 CB-J-10612 CB-N-10920 CATERINO & SPERLING (1999) CATERINO & SPERLING (1999) PASHLEY & KE (1992) VOGLER & DESALLE (1993) PALUMBI (1996) PALUMBI (1996) WHITE et al. (1990) WHITE et al. (1990) TAC AAT TTA TCG CCT AAA CTT CAG CC (CCC) GGT AAA ATT AAA ATA TAA ACT TC TAG AAT TAG AAG ATC AAC CAG C ACA TGA TCT GAG TTC AAA CCG G CCA TCC AAC ATC TCA GCA TGA TGA AA CCC TCA GAA TGA TAT TTG TCC TCA GCA TCG ATG AAG AAC GCA GC TCC TCC GCT TAT TGA TAT GC * according to the positions in Drosophila yakuba (see CLARY & WOLSTENHOLME, 1985) Fig. 28. The mitochondrial genome of Drosophila yakuba (copied from GenBank). Hatched sections of the genes Cytochrome Oxidase I (COX1), Cytochrome b (CYTB) and NADH dehydrogenase subunit 1 (ND1) were sequenced. 38 A molecular phylogeny of Agrodiaetus For the mitochondrial genome, primers k698 and Nancy were used to amplify a 700bp fragment of cytochrome oxidase subunit 1 (COI), primers Faw ND1 and 16sb were used to amplify a 530bp fragment of NADH dehydrogenase subunit 1 (ND1) and 16s rRNA and primers cb1l and cb2-h amplified a 284bp fragment of cytochrome b gene (Cyt b). Primers ITS-3 and ITS-4 were used to amplify the complete internal transcribed spacer 2 (ITS-2) in the nuclear genome together with fractions of the flanking regions of the genes coding for the ribosomal subunits 5.8s and 28s. COI was sequenced for 150 taxa and the number of specimens and populations for each country are shown in Tab. 3. ITS-2 was sequenced for 125 taxa only because distantly related outgroups turned out to be non-alignable. ND1 was only sequenced for 23 representative taxa and Cytb for a mere 5 taxa. PCR was conducted on thermal cyclers from BIOMETRA® (models UNO II or T-GRADIENT) or ABI BIOSYSTEMS® (model GENEAMP® PCR-System 2700) using the following profiles: COI & Cyt b: initial 4 minutes denaturation at 94°C and 35 cycles of 30 seconds denaturation at 94°C, 30 seconds annealing at 55°C and 1 minute extension at 72°C. ND1: Touchdown PCR with initial 3 minutes denaturation at 94°C, 15 cycles of 30 seconds denaturation at 92°C, 45 seconds annealing at 60°C with a 1°C decrease each cycle down to 45°C and 45 seconds extension at 72°C, followed by 20 cycles of 30 seconds denaturation at 92°C, 30 seconds annealing at 50°C and 45 seconds extension at 72°C. ITS-2: initial 3 minutes denaturation at 94°C and 36 cycles of 35 seconds denaturation at 94°C, 30 seconds annealing at 48°C and 1 minute extension at 72°C. PCR products were purified using purification kits from PROMEGA® or SIGMA® and checked with agarose gel electrophoresis before and after purification. Cycle sequencing was carried out on BIOMETRA® T-GRADIENT or ABI BIOSYSTEMS® GENEAMP® PCR-System 2700 thermal cyclers using sequencing kits of MWG BIOTECH® (for LI-COR® automated sequencer) or ABI BIOSYSTEMS® (for ABI® 377 automated sequencer) according to the manufacturers’ protocols and with the following cycling times: initial 2 minutes denaturation at 95°C and 35 cycles of 15 seconds denaturation at 95°C, 15 seconds annealing at 49°C and 15 seconds extension at 70°C. Primers used were the same as for the PCR reactions for the ABI (primer 1 was used for forward and primer 2 for independent reverse sequencing), but for LI-COR truncated and labelled primers were used with 3 bases cut off at the 5’ end and labelled with IRD-800. For ABI sequencing the products were cleaned using an ethanol precipitation protocol. Electrophoresis of sequencing reaction products was carried out on LI-COR® or ABI® 377 automated sequencers using the manufacturer’s protocols. Sequence alignment Alignment of the mitochondrial gene sequences COI, ND1 and Cyt b was done by hand in comparison with sequences obtained from GenBank. Some of these were also included in the analysis as outgroups (Tab. 5): 39 Chapter 3 Tab. 5. GeneBank sequences used as outgroups Gene Family Species COI COI COI COI COI COI COI,ND1,Cytb Cytb ND1 ND1 ND1 ND1 ND1 ND1 ND1 Lycaenidae Nymphalidae Papilionidae Papilionidae Pieridae Riodinidae Drosophilidae Pyralidae Libytheidae Lycaenidae Lycaenidae Nymphalidae Papilionidae Pieridae Riodinidae Euphilotes bernardino Coenonympha tullia Papilio machaon Iphiclides podalirius Colias eurytheme Apodemia mormo Drosophila yakuba Ostrinia nubilalis Libytheana bachmanii Celastrina ladon Incisalia spec. Danaus plexippus Papilio machaon Phoebis sennae Apodemia mormo GenBank accession number AF170864 AF170860 AF044006 AF170873 AF044007 AF170863 NC001322 AF442957 U32463 U32455 U32461 U32457 AJ224107 U25875 U32452 Alignment was unambiguous and straightforward, due to the conserved amino acid coding triplet pattern, and apart from one insertion event (triplet) in one Cyt b sequence, no indels were found. The 16s rRNA sections of the ND1-16s sequences were discarded from the analysis due to poor quality and alignment ambiguities. Alignment of ITS-2 sequences was done with the help of ClustalX (THOMPSON et al., 1997), but manual re-editing was required to improve the alignment. Indel events are very common in ITS-2 regions which makes alignment difficult in distantly related taxa. Therefore ITS-2 has only been used in closely related species and genera, although the discovery of secondary structures for its transcripts might make it more versatile for phylogenic studies at deeper taxonomic levels in future (COLEMAN, 2003). Alignment of ingroup sequences was mostly straightforward due to their similarity, but distant outgroups had so many indels that they had to be excluded from the analysis and (with one exception) only sequences of the subtribus Polyommatiti and one outgroup taxon (Tarucus theophrastus) were retained to avoid the exclusion of variable portions deemed to be important for phylogenetic reconstruction of Polyommatus and closely related genera. Only one section of ITS-2 had to be exluded from the analysis due to alignment problems (characters 566-588). Because of alignment problems no ITS2-sequences from GenBank were included in the analysis. (The only Lepidopteran ITS2-sequences in GenBank are from the butterfly family Papilionidae). Choice of methods for phylogenetic inference Phylogenies are usually presented in a tree and the choice of tree-building methods is a subject of heated debate between proponents of these methods. Even though different methods differ in their efficiency to reconstruct the true tree, their success depends on so many conditions that it is hardly possible to tell which method is appropriate in real data sets. Most often, the decision will have to be pragmatic and a comparison of different methods will 40 A molecular phylogeny of Agrodiaetus show that differences are confined to branches which have weak statistical support (PAGE & HOLMES 1998; NEI & KUMAR 2000). Distance methods such as UPGMA or Neighbour Joining are very fast, but less robust than other methods if underlying assumptions are violated. They also discard a lot of information because they calculate trees from distances matrices. Therefore they are not shown here. Maximum parsimony (MP) is one of the most widely used tree-building methods in phylogenetic systematics. It uses all available information from nucleotide data to infer the shortest tree. However, it is difficult to test the robustness of the tree. The most commonly used method is the bootstrap, which generates pseudoreplicates from sequence data, but bootstrap values are difficult to interprete and values are often unreasonably low in data sets of closely related species with low divergence. Despite of these drawbacks, MP-trees are presented here. Maximum likelihood (ML) is a more general approach than MP and requires an explicit model of evolution. It is computationally very expensive, especially if bootstrap values need to be computed, and therefore currently unsuitable for large data sets. The Bayesian approach has only recently been applied for phylogenetic reconstruction but already proved to be very efficient. It is related to ML but much faster to compute. Its beauty lies in the statistical properties which allow the calculation of the robustness of clades very well. Therefore this method was chosen for most discussions of phylogenetic relationships. Tree-building methods are only effective in inferring hierarchical relationships but they produce unresolved trees if recombination occurs between taxa, e.g. due to hybridization events, or if ancestral haplotypes survive in extant taxa. Such events have to be expected in radiations of closely related species such as Agrodiaetus. Network approaches are more appropriate in these cases although they have only rarely been used so far in phylogenetic systematics (POSADA & CRANDALL 2001). Different methods exist to infer networks. The Statistical parsimony approach, which is chosen here, connects haplotypes in a stepwise fashion emphasizing the similarities between them. Sequence comparisons and phylogenetic inference Sequence statistics (base composition, number of variable and parsimony-informative sites) were calculated with MEGA Version 2.1 (KUMAR et al., 2001). A saturation analysis for the different positions of COI was conducted with the help of MEGA (KUMAR et al., 1995) plotting transitions against transversions (with and without ATtransversions) and AT-transversions against CG-transversions. COI and ITS-2 datasets were tested for incongruence with the incongruence length difference test (FARRIS et al., 1995) implemented in PAUP 4.0 beta10 (SWOFFORD, 1998) as the “partition homogeneity test”. A Bayesian phylogeny reconstruction was done with the computer program MrBayes (HUELSENBECK & RONQUIST 2001). Codon positions of the COI and ND1 datasets were partitioned to allow them to have different rates of evolution and in the combined analyses genes were also partitioned to allow for different rates. The standard 4by4 nucleotide substitution model was applied, all substitution types were allowed to be different (General 41 Chapter 3 Time Reversible model) and the rates of variable sites were drawn from a gamma distribution (INVGAMMA option) with the continuous gamma distribution broken into four categories of equal weight. The Metropolis-coupled Markov chain Monte Carlo simulation (MCMCMC or (MC)3) was run under standard settings with 1,000,000 generations and every 100th tree was saved. Trees obtained before stationarity of the chain was achieved were discarded (burn in). The programme calculates branch lenghths which were multiplied with the factor 1,000,000 to enable input into the programme TreeExplorer (written by Koichiro Tamura, Tokyo). The posterior clade probabilities can be used to infer the confidence of each clade, and these values are placed at the nodes. The tree obtained from the COI data set was rooted with Apodemia mormo (C. & R. Felder, 1859) as outgroup. This species is a representative of the family Riodinidae which is appears to be the sister family of Lycaenidae according to molecular results (CAMPBELL et al. 2000) and traditional classification (ELIOT 1973). The trees from the ITS2- and the combined data sets were rooted with Tarucus theophrastus as outgroup. This species belongs to the subtribus Taruciti which turned out to be the subtribus most closely related to Polyommatiti in the COI analysis. For a phylogenetic reconstruction under a maximum parsimony approach a heuristic search for the most parsimonious tree (HSEARCH) was carried out with PAUP after the exclusion of uninformative characters (PAUP command: EXCLUDE UNINF) under the following settings: • Maximum number of trees per replicate restricted to 100 (SET MAXTREES=100) • Random addition of sequences (ADDSEQ=RANDOM) • Branch swapping method “Tree Bisection Reconnection” (SWAP=TBR) Due to a bug in the current PAUP version, only one replicate is calculated if the number of trees found is higher than “MAXTREES”, even if the number of replicates is set to a higher value. Therefore the PAUP analysis was repeated 1000 times to receive 1000 replicates. The restriction on the maximum number of trees was necessary to prevent the calculation of millions of rearrangements in the tips of the tree. Experiments with higher limits than 100 were conducted but did not improve the results. This analysis was repeated for each gene separately and with the different combinations of ITS-2, COI and ND1. Consensus trees (strict, semistrict, majority rule & Adams consensus) were calculated from the shortest trees of each replicate and from the shortest trees of all replicates together. Bootstrap analyses were done with PAUP with the heuristic search option employed, random addition of sequences, TBR branch swapping and 1000 replicates. Gene genealogies of closely related species groups were estimated with statistical parsimony as described by TEMPLETON et al. (1992) and implemented in the computer program TCS (CLEMENT et al., 2000). Because of the sensitivity of this approach to missing character information the analysis of the ITS-2 data set was repeated excluding 8 sequences with more than 10% missing characters (AD98001, AD98018, MW00110, MW00269, MW00316, MW01001, MW01025, MW99292). This data set was analyzed in two ways: counting gaps as missing character information and for the ingroup Agrodiaetus also counting gaps as 5th character. 42 A molecular phylogeny of Agrodiaetus Results Sequences and alignment The following mitochondrial gene sequences were obtained and aligned successfully: 690bp of Cytochrome Oxidase I (COI), 275bp of Cytochrome b (Cytb) and 230bp of NADH dehydrogenase subunit 1 (ND1), homologous to the following sites of Drosophila yakuba mitochondrial genome (CLARY & WOLSTENHOLME 1985): 1474-2163, 10650-10924 and 12391-12620, respectively. Of the nuclear genome, a 120bp fraction of the 5.8s ribosomal subunit and the complete internal transcribed spacer 2 (ITS-2), an aligned 672bp, were aligned successfully. ITS-2 consists of several conservative sections with highly variable sections in between, including some sections with repetitive motifs similar to microsatellites. Indels are common and length variation in ITS-2 turned out to be considerable within Lycaenidae. The shortest sequence was found in Tarucus theophrastus Fabricius, 1793 (MW02025) with 369bp while Lysandra corydonius Herrich-Schäffer, [1852] (MW99514) had the longest with 521bp. The long sequences of many species of the subgenus Lysandra were mainly due to repetitive insertions at position 19 in the aligned sequences made up of the two motifs “GTC” (repeated up to 9 times) and “CACGGCG” (repeated up to 3 times). Within the other Polyommatus subgenera, length variation was much less pronounced, with sequences varying between 439bp in Polyommatus cornelia Freyer, [1850] and 484bp in Polyommatus thersites Cantener, [1835]. Variation within Agrodiaetus was similar. Most sequences were between 443bp (Agrodiaetus maraschi Forster, 1956) and 471bp (Agrodiaetus gorbunovi Dantchenko & Lukhtanov, 1994 & A. wagneri Forster, 1956). Only Agrodiaetus dama Staudinger, 1892 had a longer sequence of 482bp. In the distantly related outgroups (which were not included in the further analysis due to alignment problems) the longest ITS-2 sequence was found in Iphiclides podalirius L., 1758 (JC02001) from the family Papilionidae with 536bp. In this family an even more extreme length variation has been found in the genus Luehdorfia, (sequences taken from GenBank): 687bp in Luehdorfia chinensis Leech, 1893 (AB071925) compared to only 362bp in Luehdorfia japonica Leech, 1889 (AB071910). Base composition and sequence divergence Base composition in the insect mitochondrial genome is known to be heavily AT-biased, especially in 3rd codon positions, while the nuclear genome has a more homogenous base ratio. This also holds for the investigated gene sections in Lycaenidae (Tab. 6). In ITS-2 no base heterogeneity is found in Iphiclides podalirius L. (Papilionidae), but in Lycaenidae a slight deficiency in Adenin (A) is covered by a surplus in Guanin (G). This base composition is especially pronounced in the whole tribus Polyommatini and is remarkably constant in all taxa investigated. In the mitochondrial genes, the AT bias ranges from between 59% and 73% in 1st and 2nd codon positions (lowest in COI and highest in ND1) to 90% in 3rd codon positions of all three genes. Guanin (G) is especially rare in 3rd codon positions of genes on the (+)strand (COI, Cytb) while Cytosin (C) is rarest in ND1 which is on the (–)strand. The AT bias at 3rd codon positions of COI is less pronounced in the genus Polyommatus (90%) compared to other genera of the family Lycaenidae (94%). Thus the AT bias in Polyommatus is similar to other insect groups, but not as extreme as in the genus Arhopala (Lycaenidae) with average values over 95% (MEGENS 2002). 43 Chapter 3 Tab. 6. Base composition (Nucleotide frequencies with standard errors) Gene COI (Polyommatus) 1st Position 2nd Position 3rd Position COI (other Lycaenidae) 1st Position 2nd Position 3rd Position ND-1 (Polyommatus) 1st Position 2nd Position 3rd Position Cytb (Lycaenidae) 1st Position 2nd Position 3rd Position ITS-2 (Polyommatini) (Polyommatus) (other Polyommatini) (other Lycaenidae) (Iphiclides podalirius) T 37.22 28.70 42.50 40.49 39.39 29.78 42.65 45.78 45.31 41.20 46.76 48.10 43.18 37.55 45.33 46.68 23.15 23.19 22.60 23.91 26.8 C 0.65 0.87 0.40 1.58 1.16 1.09 0.56 2.83 0.53 0.76 0.61 1.18 0.67 0.19 0.62 2.32 0.58 0.58 0.37 1.86 15.48 13.91 25.10 7.42 13.84 12.72 24.67 4.11 9.40 11.06 15.57 1.48 10.88 7.68 16.88 8.05 25.95 25.95 26.02 25.09 24.7 A 0.60 0.83 0.37 1.25 0.67 0.92 0.44 1.52 0.51 0.78 0.50 1.22 0.51 0.78 0.64 1.59 0.63 0.63 0.64 1.62 32.39 32.27 15.92 49.03 35.52 32.88 16.20 48.54 32.98 31.49 25.99 41.67 32.20 32.90 21.00 42.78 19.67 19.64 20.10 22.83 22.2 G 0.52 0.59 0.45 1.34 0.98 0.84 0.51 2.77 0.79 1.22 0.33 2.70 0.57 0.42 0.20 2.01 0.86 0.82 1.19 1.62 14.90 25.13 16.49 3.07 14.22 24.62 16.48 1.56 12.30 16.34 11.78 8.75 13.83 21.93 16.88 2.50 31.23 31.22 31.27 28.17 26.3 0.44 0.66 0.33 1.06 0.43 0.77 0.39 0.90 0.70 1.10 0.28 2.77 0.68 0.30 0.35 2.13 0.69 0.69 0.70 1.34 Tab. 7. Variable and parsimony-informative sites in single gene studies Gene COI Taxa Papilionoidea Lycaenidae Polyommatini Polyommatiti Polyommatus Agrodiaetus ND1 Lycaenidae Polyommatus Agrodiaetus Cytb Lycaenidae ITS2 Polyommatiti (gaps= Polyommatus missing) Agrodiaetus 5.8s Polyommatini Sequences 318 310 297 282 261 200 25 22 15 6 199 185 138 200 nucleotides variable sites 326 47.2% 311 45.1% 300 43.5% 284 41.2% 267 38.7% 223 32.3% 76 33.0% 58 25.2% 38 16.5% 53 19.2% 257 39.6% 238 36.7% 159 24.5% 9 7.5% parsimonyinformative 274 39.7% 260 37.7% 249 36.1% 237 34.3% 219 31.7% 172 24.9% 45 19.6% 33 14.3% 23 10.0% 15 5.4% 154 23.7% 132 20.3% 76 11.7% 4 3.3% amino acids variable parsimonysites informative 74 32.2% 38 16.5% 68 29.6% 34 14.8% 63 27.4% 32 13.9% 56 24.3% 32 13.9% 49 21.3% 29 12.6% 37 16.0% 17 7.4% 18 23.7% 8 10.5% 10 13.2% 6 7.9% 8 10.5% 2 2.6% 8 8.7% 3 3.3% The number and percentage of variable and parsimony-informative sites for each gene is given in Tab. 7. Figures are for different taxonomic levels, from the superfamily (Papilionoidea) down to the ingroup (subgenus Agrodiaetus). The variation in amino acids of mitochondrial genes is much lower than the variation in nucleotide sites because most variation is found in synonymous sites (especially 3rd codon positions). The section of 5.8s rRNA turned out to be almost invariable in Lycaenidae and was therefore lumped with ITS-2 44 A molecular phylogeny of Agrodiaetus in the phylogenetic analysis. The comparison of sequence variation in the combined gene analyses (Tab. 8) reveals that Cytb and ND1 evolve faster than COI. This difference is almost entirely due to the higher number of nonsynonymous substitutions. Tab. 8. Variable and parsimony-informative sites in combined gene studies Gene ND1 COI Cytb COI COI ITS-2 5.8s Taxa Lycaenidae Lycaenidae Lycaenidae Lycaenidae Polyommatini Polyommatini Polyommatini Sequences 25 25 6 6 200 200 200 nucleotides variable sites 76 33.0% 200 29.0% 53 19.2% 109 15.8% 276 40.0% 277 42.7% 9 7.5% parsimonyinformative 45 19.6% 116 16.8% 15 5.4% 24 3.5% 218 31.6% 156 24.0% 4 3.3% amino acids variable parsimonysites informative 18 23.7% 8 10.5% 30 13.0% 9 3.9% 8 8.7% 3 3.3% 12 5.2% 1 0.4% 51 22.2% 27 11.7% The saturation analysis showed no saturation in 1st and 2nd codon positions of COI within Lycaenidae, because the number of transitions is linearly increasing with the number of transversions (Fig. 29). In the 3rd codon positions transitions were saturated in ingroupoutgroup comparisons (Fig. 30) but there is still a high number of transversions. Due to the AT bias most of these are AT-transversions as can be seen from Fig. 31 in which ATtransversions were excluded. Fig. 32 displays the ratio of AT-transversions against CGtransversions. The steep linear increase indicates that AT-transversions are not saturated in ingroup-outgroup comparisons. Fig. 29. Ratio of transitions (ti) and transversions (tv) in 1st and 2nd codon positions of COI in all investigated taxa 45 Chapter 3 Fig. 30. Ratio of transitions (ti) and transversions (tv) in 3rd codon positions of COI within Agrodiaetus (ingroup) and compared to the outgroups (other taxa). Fig. 31. Ratio of transitions and transversions excluding AT-transversions in 3rd codon positions of COI within Agrodiaetus (ingroup) and compared to the outgroups (other taxa). 46 A molecular phylogeny of Agrodiaetus Fig. 32. Ratio of AT-transversions and other transversions in 3rd codon positions of COI within Agrodiaetus (ingroup) and compared to the outgroups (other taxa). The partition homogeneity test revealed a significant incongruence between the COI and ITS-2 data sets (p<0.001). Despite this result, both data sets were combined for a total evidence approach, but they were also analyzed separately to figure out the incongruence in an effort to elucidate deviations of the gene trees from the true species tree. 47 Chapter 3 Bayesian inference of phylogeny The phylogenetic trees inferred from the Bayesian approach are represented in Fig. 33 - Fig. 46 for the COI data set, Fig. 47 & Fig. 48 for ITS-2 and Fig. 49 - Fig. 57 for the combined data set. Only the COI data set can be used to infer the phylogeny above the level of the subtribus (Polyommatiti). 98 100 61 Polyommatini Theclini Eumaeini Lycaenini Riodinidae 100 100 500000 Fig. 33. MrBayes, COI, Lycaenidae (relationships of tribes) The basal splits in the COI tree are on the tribus level (Fig. 33), separating the monophyletic Lycaena (Lycaenini), Satyrium (Eumaeini) and Favonius (Theclini) from the other taxa of tribus Polyommatini). Relationships within the genus Lycaena are highly resolved and shown in Fig. 34. The most basal taxa (thetis, asabinus, thersamon) belong to the thersamon-group (HESSELBARTH et al. 1995). 98 Polyommatini 100 Favonius quercus Satyrium hyrcanicum 61 Satyrium esculi 100 Satyrium myrtale 84 Lycaena thetis Lycaena asabinus 100 Lycaena thersamon Lycaena candens 93 98 99 Lycaena virgaureae 57 Lycaena tityrus Lycaena alciphron heracleana 100 100 Lycaena alciphron melibaeus 98 Lycaena phlaeas Apodemia mormo 500000 Fig. 34. MrBayes, COI, Lycaenidae (excl. Polyommatini) Within Polyommatini the splits follow the level of morphologically defined subtribes but their relationships are not well resolved (Fig. 35). 91 100 Taruciti Celastriniti 98100 100 Scolitantiditi Glaucopsychiti 92 98 Lampiditi 100 Cacyreus Everiti Leptotiti Zizeeriti Theclini Eumaeini Lycaenini Riodinidae 88 95 32 51 98 100 83 61 100 100 500000 Fig. 35. MrBayes, COI, Lycaenidae (subtribes) 48 Polyommatiti A molecular phylogeny of Agrodiaetus The subtribus most closely related to Polyommatiti are Taruciti, Celastriniti, Scolitantiditi, Glaucopsychiti and Lampiditi (Fig. 36, Fig. 37). 95 98 98 100 100 Polyommatiti 100 Celastriniti Taruciti 100 Scolitantiditi Glaucopsychiti 98 Lampiditi 100 Cacyreus Everiti Leptotiti Zizeeriti Theclini Eumaeini 100 Lycaenini 100 Riodinidae Fig. 36. MrBayes, COI, Lycaenidae (subtribes), condensed tree at 95% confidence limit 100 Polyommatiti Celastrina argiolus mauretanica Celastrina argiolus argiolus 88 98 Tarucus theophrastus Pseudophilotes abencerragus 100 Pseudophilotes vicrama 95 Maculinea arion 92 Turanana endymion 98 Lampides boeticus (Spain) 32 100 Lampides boeticus (Morocco) 51 83 Lampides boeticus (Turkey) Cacyreus marshalli 98 Cupido osiris Leptotes pirithous 100 Zizeeria knysna 83 Theclini Eumaeini 61 100 Lycaenini 100 Riodinidae 91 100 500000 Fig. 37. MrBayes, COI, Polyommatini excl. Polyommatiti Within Polyommatiti, the genus Chilades and the North American Euphilotes appear as the most basal taxa in the tree and the other taxa of the two genera Polyommatus and Plebeius together form a monophyletic unit (Fig. 38, Fig. 39). 100 Polyommatus Plebeius (Agriades) pyrenaicus Plebeius (Aricia) anteros 88 Plebeius (Aricia) cram era 72 Plebeius (Aricia) ages tis complex 28 99 Plebeius (Aricia) torulensis 100 Plebeius (Aricia) isau ricus 100 Plebeius (Aricia) eum e don 42 Plebeius (Plebejides) pylaon 100 94 Plebeius (Kretania) eurypilus 96 Plebeius (Vacciniina) alcedo (Iran) 37 24 100 Plebeius (Vacciniina) alcedo (Turkey) Plebeius (Vacciniina) morgianus Plebeius (Plebejidea) loewii 65 100 Cyaniris semiargus persica 32 100 Cyaniris semiargus m aroccana Plebeius (Plebeius) argus Plebeius (Plebeius) christophi 100 Euphilotes bernardino Chilades trochylus 40 200000 Fig. 38. MrBayes, COI, Polyommatiti 49 Chapter 3 88 99 100 94 100 100 96 100 100 65 100 100 100 Polyommatus Plebeius (Agriades) pyrenaicus Plebeius (Aricia) anteros 72 Plebeius (Aricia) cramera Plebeius (Aricia) agestis complex Plebeius (Aricia) torulensis Plebeius (Aricia) isauricus Plebeius (Aricia) eumedon Plebeius (Plebejides) pylaon Plebeius (Kretania) eurypilus Plebeius (Vacciniina) alcedo (Iran) Plebeius (Vacciniina) alcedo (Turkey) Plebeius (Vacciniina) morgianus Plebeius (Plebejidea) loewii Cyaniris semiargus persica Cyaniris semiargus maroccana Plebeius (Plebeius) argus Plebeius (Plebeius) christophi Euphilotes bernardino Chilades trochylus Fig. 39. MrBayes, COI, Polyommatiti, condensed (50% level) While the genus Polyommatus (excluding the subgenus Cyaniris) also forms a monophyletic unit, the monophyly of the genus Plebeius is not supported and the relationships of most of its taxa are not well resolved. One exception is the subgenus Aricia (excluding A. eumedon) whose monophyly and taxa relationships appear quite well resolved. This genus includes the Aricia agestis-complex, a group of closely related taxa (agestis, artaxerxes, montensis, Fig. 40), but Aricia cramera (Eschscholtz, 1821) which is also thought to belong to this complex and phenotypically very similar, appears very distant genetically. 83 100 100 Aricia montensis (Spain) Aricia montensis (Morocco) Aricia artaxerxes (Greece) Aricia agestis (Turkey) Aricia artaxerxes (Greece) Aricia agestis (Greece) Aricia agestis (Iran) Fig. 40. MrBayes, COI, Aricia agestis complex, condensed (50% level) 99 Agrodiaetus Neolysandra corona Neolysandra fatima 69 99 100 Polyommatus eroides forsteri Polyommatus menelaos 53 40 Polyommatus eroides eroides 31 100 Polyommatus eros yildizae 85 Polyommatus icarus 100 100 Polyommatus dorylas 100 Polyommatus icarus (Morocco) 100 36 Meleageria daphnis 100 27 Polyommatus escheri 21 41 Polyommatus thersites 45 100 Polyommatus amandus amandus Polyommatus amandus83 abdelaziz 100 Polyommatus myrrhinus 100 Polyommatus cornelia 85 99 Polyommatus aedon Neolysandra coelestina Neolysandra diana 93 39 Lysandra 100 100 200000 Fig. 41. MrBayes, COI, Polyommatus 50 A molecular phylogeny of Agrodiaetus 100 69 99 53 100 85 100 100 100 99 93 99 Agrodiaetus Neolysandra corona Neolysandra fatima 100 Polyommatus eroides forsteri Polyommatus menelaos Polyommatus eroides eroides Polyommatus eros yildizae Polyommatus icarus 100 Polyommatus dorylas 100 Polyommatus icarus (Morocco) 100 100 Meleageria daphnis Polyommatus escheri Polyommatus thersites 100 Polyommatus amandus amandus Polyommatus amandus abdelaziz 83 Polyommatus myrrhinus Polyommatus cornelia 85 Polyommatus aedon Neolysandra coelestina Neolysandra diana Lysandra 100 Fig. 42. MrBayes, COI, Polyommatus, condensed (50% level) 99 Agrodiaetus Neolysandra corona Neolysandra fatima 99 69 100 Polyommatus eroides forsteri Polyommatus menelaos 53 40 Polyommatus eroides eroides 100 Polyommatus eros yildizae Polyommatus icarus (Spain) 31 85 Polyommatus andronicus 100 Polyommatus icarus (Turkey) 69 Polyommatus icarus (Greece) 67 29 Polyommatus icarus (Iran) Polyommatus dorylas (Spain) 100 Polyommatus dorylas (SW Turkey) 100 Polyommatus dorylas (NE Turkey) 100 Polyommatus icarus (Morocco) 73 Meleageria daphnis marcida 62 Meleageria daphnis versicolor 100 Meleageria daphnis versicolor 27 Meleageria daphnis brandti 36 87 Meleageria daphnis marcida 100 Polyommatus escheri (Greece) 21 Polyommatus escheri (Spain) Polyommatus thersites (NE Turkey) 41 45 Polyommatus thersites (Spain) 100 thersites (Iran) 71 Polyommatus 35 Polyommatus thersites (SW Turkey) Polyommatus amandus amandus Polyommatus amandus abdelaziz 97 Polyommatus myrrhinus (Erzurum Prov.) 83 Polyommatus myrrhinus (Erzurum Prov.) Polyommatus myrrhinus (Erzurum Prov.) 100 37 Polyommatus cornelia (Kayseri Prov.) 100 Polyommatus cornelia (Adana Prov.) Polyommatus cornelia (Erzincan Prov.) 85 99 Polyommatus aedon aedon Neolysandra coelestina Neolysandra diana 39 93 Lysandra 100 100 200000 Fig. 43. MrBayes, COI, Polyommatus Within the genus Polyommatus (Fig. 41, Fig. 42) the subgenera Agrodiaetus (sensu ECKWEILER & HÄUSER 1997) and Lysandra appear clearly as monophyletic units. The 51 Chapter 3 unification of Meleageria with Lysandra is not supported. The subgenus Neolysandra splits into two separate clusters. One of them, which consists of the species N. corona (Verity, 1936) and N. fatima (Eckweiler & Schurian, 1980), forms the sister clade to Agrodiaetus, the other (with N. coelestina (Eversmann, 1843) and N. diana Miller, 1913) clusters with the P.aedon/myrrhinus/cornelia-group. This split is also supported by genital morphology (COUTSIS 2001) which also suggests a closer relationship of N. corona and N. fatima to P. dorylas ([Denis & Schiffermüller], 1775) than to N. coelestina and N. diana. Some species (P. icarus, P. eroides, P. cornelia) do not appear as monophyletic units in the COI gene tree (Fig. 43) and P. myrrhinus, which is considered a subspecies of P. aedon by HESSELBARTH et al. (1995), clusters with P. cornelia. The genus Lysandra constitutes a parallel case compared to Agrodiaetus with closely related taxa differing in (mostly high) chromosome numbers. Lysandra syriaca appears at the basis but the relationships of the other taxa of the coridon group remains unresolved or obscure and only the populations of Lysandra bellargus form a well supported monophyletic clade (Fig. 44, Fig. 45). 100 46 86 98 Lysandra syriaca Lysandra ossmar (Turkey: Isparta) Lysandra corydonius (Turkey: Erzurum) 100 Lysandra corydonius (Turkey: Igdir) 99 Lysandra corydonius (Turkey: Igdir) Lysandra ossmar (Turkey: Sivas) Lysandra albicans (Spain: Huesca) Lysandra albicans (Spain: Huesca) 96 Lysandra albicans (Spain: Soria) 60 Lysandra coridon (Spain: Burgos) 39 Lysandra gennargenti (Italy: Sardinia) 61 100 Lysandra coridon (Spain: Barcelona) Lysandra caelestiss imus (Spain: Teruel) 70 Lysandra coridon (Spain: Tarragona) 53 Lysandra corydonius (Turkey: Erzurum) 98 Lysandra coridon (Italy: Aosta) 100 23 Lysandra bellargus (Iran) 21 Lysandra bellargus (Italy) 51 Lysandra bellargus (SE Turkey) Lysandra bellargus (SW Turkey) 100 Lysandra bellargus (Spain) 100000 Fig. 44. MrBayes, COI, Lysandra 100 99 86 100 96 60 61 98 100 70 53 98 100 51 100 Lysandra syriaca Lysandra ossmar (Turkey: Isparta) Lysandra corydonius (Turkey: Erzurum) Lysandra corydonius (Turkey: Igdir) Lysandra corydonius (Turkey: Igdir) Lysandra ossmar (Turkey: Sivas) Lysandra albicans (Spain: Huesca) Lysandra albicans (Spain: Huesca) Lysandra albicans (Spain: Soria) Lysandra coridon (Spain: Burgos) Lysandra gennargenti (Italy: Sardinia) Lysandra coridon (Spain: Barcelona) Lysandra caelestissimus (Spain: Teruel) Lysandra coridon (Spain: Tarragona) Lysandra corydonius (Turkey: Erzurum) Lysandra coridon (Italy: Aosta) Lysandra bellargus (Iran) Lysandra bellargus (Italy) Lysandra bellargus (SE Turkey) Lysandra bellargus (SW Turkey) Lysandra bellargus (Spain) Fig. 45. MrBayes, COI, Lysandra, condensed (50% level) The condensed tree for the Agrodiaetus clade (with 95% confidence limit) is presented in Fig. 46. The following clades are well supported: • antidolus-group (antidolus, kurdistanicus, femininoides, morgani, peilei, karindus) • elbursicus-group (elbursicus, zarathustra, arasbarani, paulae) 52 A molecular phylogeny of Agrodiaetus • • • • • 21 C s si n e 90 an i C 21 ad m a rian e C 23 a m u a C h A. .da sch rce ae 7 76 5 A A. .pie rce 9 26 20 1X A .pie i C3 s C2 6 7 0 E 9 8 2 6 2 X A i l e lu C 6 C 2 W W 98 929 41X A.pe ntido dus ides M W 9 93 1 .a rin no s M W 9 59 A ka ini ide 0 M W 02 14 A. em ino C3 M E 026 12 A.f min lus C44 65 W E 26 26 .fe tido lus C W E0 02 1 A .an tido nicus 5 W W0 267 6 A .an ista C5 M E0 937 X A .kurd nicus 2 W W9 9406 X A rdista s C4 M W9 473 .ku idolu M W99 286 A A.ant M W99 93X M 993 MW • carmon-group (carmon, surakovi, sekercioglui) admetus-group (admetus, ripartii, nephohiptamenos, demavendi, lorestanus, khorasanensis) iphidamon-group (iphidamon, dizinensis) menalcas-group (menalcas, alcestis, interjectus, dantchenkoi, aroaniensis, humedasae, valiabadi, fabressei, ainsae, fulgens) poseidonides-group (poseidonides, dagmara) baytopi-group (baytopi, rovshani, tankeri, iphicarmon) erschoffii-group (erschoffii, tenhageni, achaemenes, shahrami, glaucias, phyllis, klausschuriani, posthumus, darius, caeruleus, birunii) poseidon-group (poseidon, putnami, hopfferi, lycius, actis, firdussii, sigberti, pseudactis, artvinensis, ernesti, damalis, merhaba, mithridates, wagneri, altivagans, sertavulensis, merhaba, mofidii, pseudoxerxes, gorbunovi, kanduli, sennanensis, cyaneus) MW99094x A.cy aneus C17 MW99448 A.cyaneus C18 MW99059X A.merha ba C17 MW9944 MW001799 A.cyaneus X A.cyane MW0033 us 0 A.pse WE0 u MW 2621X A.s doxerxes C15 MW 99357 A ennanen MW900178 A .altivagans sis MW 9465 .gorbu M 0012 A.kan novi WEW0017 9x A.go duli C25 rbu M 026 7X A W W99 75 A .gor novi C WEE023353 A .gorbu bunovi 20 C2 . no 2 alt M 02 1 M W9 421 A.p ivag vi C1 0 MWW988136 A.m seud ans C 9 o o 2 M 3 X x 2 f M W 991 13 A.w idii erxes M W 99 65 A.s ag W W 98 240 X A ert ne M E0 982 170 A.a .alti avule ri C1 8 A W 2 v M D9 99 53603X A.waltiva aga nsis W 80 05 A A g ga ns C2 98 1 7 .d .m ne ns C2 0 r 2 i C 5 A 18 A . am thr i C 2 0 .a me al ida 16 1 A. ltiv rh is te sC po a ab se ga a C 23 id n s 1 on 7 C1 9 • • 34 0 e i na rt C C2 ni be ae la A. hu in o 8 A. A.n rcic n 01 24 X .tu mo n 90 98 80 508 9 A .da mo rtii C 0 AD 9 99 47 6 A .da pa C9 3 AD W 99 54 3 A A.ri artii tii C9 M W 99 61 8x .rip ar M W 99 06 A .rip tii M W 99 96 x A par i M 91 7 .ri rti s MWW9 94063 A .ripa stanu ndi M W9 92 4 A ore ave M W9 926 A.l .dem endi M 9 35 A av i MWE025189XA.dem avend di C66 W W00 141 .dem aven M W99 04 A .dem endi M 991 X A mav MW 991052X A.de rtii MW 9910 A.ripa anensis MW00043x .khoras di 1A ven JC a 3 4 m WE0929381 A.de avendi MW 677 A.dem avendi C70 WE02 2X A.dem 0 MW9938 3X A.demavendi C7 MW0018 A.demavendi MW00186x C70 MW00015X A.ahmadi MW00185X A.demavendi C70 JC01014X A.admetus MW98084x A.admetus MW01105 JC00045 A.ripartii JC0004 A.nephohiptamenos MW0 6X A.nepho MW 1014x A.rip hiptamenos MW 01072X A artii C90 MW 98294 A .ripartii AD 98240 .guezel MW98001 A.theres mavi iae C M 992 A.sur 59 M W990 89 A.s akovi M W98 60X ekerc M W00 009 A A.car ioglu M W0 051 .car mon i C46 M W0 0110 X A. mon C79 M W0 005 X A elbu M W0 023 8X .elb rsic M W 00 2 A A.e urs us C M W 003 59 .e lbur icus C 17 W W 00 16 X A lbur sic 1 M E 00 05 X .el sic us C1 8 W W 02 12 6X A.e bu us 8 M E 0 66 7 A lb rsic C1 M W 02 015 1 A A.pa.elbu ursic us C 8 W 00 53 7X .a u rs us 16 r la i 00 0 1 0 A A as e cu C17 0 32 1 A .za .pau bar C17 s X .ha ra la ani A. m thu e h a s a m dan tra ad e C an ns 22 e is n sis C 26 on rm i ca r 27 hi ke ip an pi i C s A. .t yto op e 3 A ba ayt nid es 10 65X A. .b ige nid des 98 5 4 A iph ige oni W 9 9 3 4 2 X A. p h i d a 7 M W 99 937 1X A.i ose mar s C2 M W 9 00 1X A.p ag cu M W 01 00 x .d rci M S 98 01 X A .tu cus C24 s A ci D E 0 5 3 W S00 000 35X A.tur urcicu ia C1 2 D S0 91 26 A.t en C1 D W9 95 3X iphig enia M 9 20 A. ig MWW999009 A.iphigenia ia C15 M W9 170 .iph igen C14 M W99 29 A A.iph enia M 980 6X phig ADW981049X A.i sae M 980 3 A.ain ens C90 MW 0105 A.fulg e MW 107 insa 1 MW001078 A.a insae MW 01001 A.a ressei MW 01 A.fab ei JM000039 A.fabress i MW01 A.valiabad MW00064 X A.valiabadi C23 MW00498 ae C44 MW99591X A.humedas MW99605 A.humedasae JC00040 A.aroaniensis MW98172X A.menalcas MW99494 MW9802 A.menalcas MW98 0 A.menalcas MW 315 A.alcestis 98 MW 212 A.alces C20 MW 99164 A.inte tis C21 rjectus MW 00229X C31 MW 00231 A.alces MW 9938 X A.alc tis C19 M 993 0X A.a estis C19 M W992 19X A lcestis M W99 74X .dantc C19 M W99 320 A A.dan henk M W99 276 .dan tche oi C4 M W0 471 X A. tche nkoi C4 2 2 M W0 026 X A dant nko M W0 032 9X .da che i C41 M W 04 8X A.ip ntch nkoi M W 005 24 A. hid .Xme C42 n M W 99 39 X A iph am M W 99 31 A .ip idam on C1 alcas o C5 M W 9 47 4X .di hid z 4 9 0 M W 9 2 6X A in am n M W 9 93 26 A .tu en on W 99 909 74 A.z .za rcico sis C C14 99 05 5 A. ap pv la 1 55 3 X A zap va adi C2 7 d 0 A 2 X .hu .hu vad i C19 A. b be i C hu er rti 19 b ti C e rti C36 34 C3 5 9 C1 5 n C2 do mi 25 1 i se na i C C2 0 po ut m n C2 A. .p tna do n 3 X A .pu sei eido C15 8 1 61 A po os ri 16 98 0 1 A. .p ffe i C W 99 50 4 A op fer M W 99 815 38X A.h opf s M W 9 81 8 .h ciu s M W 9 40 9 A .ly ciu sii M W 99 18 A .ly us C30 M W 98 079 x A .fird sii 5 M W 98 89 A dus sii C2 M W 80 25X .fir dus M W9 01 6 A .fir berti M W0 900 4X A .sig actis C25 M W9 023 X A eud nsis M W0 285 A.ps tvine M W98 09X A.ar sii C17 M 980 58X firdus ADW990 62 A. igberti 18 M 981 4 A.s esti C MW 9828 X A.ern i C24 MW 98097 A.haig 25 MW 99247X A.haigi C MW 99413X ssii MW 0151 A.firdu gi C25 MW0 422X A.hai 99 MW A.phyllis AD980360X A.phyllis MW0014 MW99174 A.phyllis MW99187 A.phyllis MW00348 A.phyllis C85 MW00452X A.phyllis C75 MW00327x MW0039 A.erschoffii WE024 3X A.erschoffii C14 WE024951 A.tenhageni 1X A.ach WE8 WE 5001X A.sh aemenes arami MW 00002X MW000259 A A.glaucias MW 0262X .klaussc 0 hu MW 0347 A.kla uss riani C5 00 XA MW 001358X A .darius churiani 7 MW C56 0 . C 85 M 00 1 A dar M W00 335 .dar ius MWW00 409XX A.ca ius er M 0 44 A M W0 026 4X .cae uleu M W 05 7 A A.po rule s C2 MWW0000647 A .biru sthu us C 0 20 0 0 .b n m M M W 00 47 A. iru ii C us W W 00 07 6X po nii 10 C11 M E0 00 102 2X A.p sthu C10 M W 2 17 X A.p os mu W 99 66 6x A o thu s 99 30 2 A .po sth m C1 55 9X A.r .ro sth um us C 1 9 A ov vsh um us 1 A. .b s h a C 1 ta ay an ni us C 11 nk to i 11 er pi i Fig. 46. MrBayes, COI, Agrodiaetus, condensed tree (95% confidence level) 53 Chapter 3 On a lower level of confidence (83%), the first three clades form a joint clade together with the taxa dama, schuriani and pierceae and, with only 75% confidence, the taxa ninae, turcicola, zapvadi, huberti and damon cluster together. The relationships between the different clades remain largely unresolved on a low level of confidence. 92 100 100 100 99 100 93 100 94 99 100 100 99 100 99 100 100 99 100 100 99 99 96 100 0 100 100 100 Agrodiaetus JC00039 P.escheri MW98235 P.cornelia MW99038 P.cornelia MW98264 P.cornelia MW00326 P.aedon MW99537 P.myrrhinus MW99550 P.myrrhinus MW02001 P.amandus MW99047 P.amandus MW00504 N.corona MW99301 N.fatima MW00076 M.daphnis MW98029 M.daphnis MW00290 M.marcida MW99013 N.coelestina MW98220 P.loewii MW99018 A.pyrenaicus MW99134 A.eumedon MW00024 V.alcedo MW99163 P.pylaon MW99303 K.eurypilus MW00525 C.semiargus MW02034 C.semiargus MW00517 V.morgianus MW01061 A.montensis MW02033 A.montensis JC00055 A.artaxerxes MW01048 A.cramera MW99097 A.isauricus MW99001 A.torulensis MW98270 A.anteros MW00116 P.argus MW00469 L.bellargus MW99608 L.bellargus MW01011 L.bellargus MW98278 L.bellargus MW99514 L.corydonius MW99536 L.corydonius MW01059 L.albicans MW98129 L.ossm ar MW98185 L.ossm ar MW98228 L.syriaca MW01116 L.coridon MW01018 L.coridon MW01092 L.albicans MW01034 L.albicans MW99612 L.coridon OK96022 L.caeles tissimus MW01019 P.dorylas MW99014 P.dorylas MW00302 P.thersites MW01083 P.thersites MW02006 P.icarus MW00412 P.icarus MW00530 P.eroides JC00029 P.menelaos JC00042 P.eroides MW01025 P.icarus JC00061 P.andronicus JC00063 P.icarus MW02025 T.theophrastus Fig. 47. MrBayes, ITS2, Polyommatiti, condensed (90% confidence level) 54 A molecular phylogeny of Agrodiaetus MW00226 A.femininoides C27 WE02614 A.morgani C27 WE02671 A.fe mininoides C2 MW9939 7 3 A.antid olus C42 MW99 4 MW99 06 A.antidolu s C4 4 MW9 286 A.kurd istanic MW 8205 A.d us C5 990 a m 5 a 95 A AD9 .hub MW 8024 e rti C A.h 34 WE 9955 ube MW 0259 2 A.hu rti MW 982 1 A.p berti C33 W 98 40 A eilei C3 M E02 294 .the 9 AD W00 661 A.gu resia e ez M 9 23 A. elm C59 M W 801 2 A ara M W9 994 8 A .elb sbar avi W 9 79 .n a u 00 50 A ina rsic ni us 05 8 A .tu e rc C1 8 A. .nin ico 8 el ae la bu C2 C rs 34 0 ic us C 18 os en am t i i p t ar ohi 90 sis n ri p h iC A. nep arti ane . 3 s p i 04 5 A A.r hora us 0 4 t k 4 0 0 1 90 . e 66 JC C00 10 1 A adm rtii C di C J W0 243 A. ripa ven i M E0 014 A. ema d n W 01 196 A.d ave JC W99 105 .dem anus M 99 89 A rest W M 001 5 A.lo mon MW 0253 6 A.da on WE 9954 A.dam MW 99613 MW The Bayesian analysis of the ITS-2 dataset does not support the monophyly of Polyommatus (Fig. 47). Relationships of Polyommatus sensu strictu appear unresolved and the Plebeius subcluster which is found within Polyommatus includes not only Cyaniris but also Lysandra. The monophyly of Aricia (excl. A. eumedon) and Lysandra are very well supported and within the latter group the taxa bellargus, corydonius and ossmar appear as monophyletic units. The monophyly of Agrodiaetus is supported with a posterior probability of 92%, but the sister group is unresolved. Within Agrodiaetus (Fig. 48), Agrodiaetus damon appears as the sister taxon to all other Agrodiaetus, but the phylogeny of the remaining taxa is mainly unresolved. 4 C1 2 a ni C1 ge ia hi en nes ip e g A. iphi em n o a 9 . 04 9 A ach am 98 0 A. h id a m i s r e W 90 1 .ip M W9 49 8 A hah onid M E02 032 A.s seid des 4 i W W0 001 .po C1 en M E85 01 A .iphig amon A id W 00 h 1 0 p DS 0100 9 A.i ucias DS 0026 A.gla ageni 17 MW 00002 A.tenh sis C WE 02451 .dizinen WE 00539 A insae MW 1001 A.a ae MW0 053 A.ains 1 MW0 1 A.fabressei 0 s JM000 7 A.fulgen MW0110 abressei MW01039 A.f MW98172 A.menalcas MW99471 A.dantch.Xmenalcas C50 MW99164 A.interjectus C31 MW99319 A.d antchenkoi C4 MW0023 2 1 A. al ce stis C19 MW99 2 MW9 74 A.dantche 8 nkoi C4 MW9 315 A.alce 2 stis C MW 9276 A.d 2 0 9 antch JC 8212 enko A 0 i C 42 MW 0040 A .alcesti MW 9959 .aroan s C21 1A ien M 99 s . h 6 is W u m MW 000 05 A. e das ae M 00 64 A hume C 38 das M W99 498 .val ae W W0 40 A.v iabad i M E0 018 7 A. alia M W0 267 5 A dem badi W 00 7 . C2 a 99 1 A. dem ven 3 5 d 3 82 A. em aven di C6 A. dem ave di C 0 n d a 70 e m ve d i n a v en d i C 7 di C7 0 0 1 5 20 C ri vi C fe pf no i 0 ho bu nov C2 i A. or .g rbu nov 8 15 40 7 A .go bu vi sC 99 17 A .gor uno erxe b W 0 78 x M W0 01 9 A gor do M W0 012 4 A. pseu us 7 e M W0 67 C1 A. an M E02 330 A.cy neus 18 W W00 179 .cya eus C M 00 94 A yan C25 MW 990 8 A.c berti 0 MW 9944 5 A.sig ssii C3 MW 9828 A.firdu C24 ii MW 99006 .firduss MW 9247 A eudactis 9 25 MW 009 A.ps ssii C 8 AD9 413 A.firdu C2 5 9 MW9 irdussii 234 A.f MW00 C17 2 A.actis MW9816 irdussii MW00151 A.f MW99058 A.artvinensis C25 MW99372 A.baytopi C27 MW99501 A.putnami C25 MW00101 A.d arius MW0034 7 A.posthu MW00 mus C85 393 A.e M rs ch W o 0 ff 0 ii C14 MW0 409 A.cae ruleus MW 0102 A.b C20 0 MW 0267 A irunii C1 1 MW 00072 .birunii C1 0 MW 0054 A.biru MW 004 7 A.bir nii C11 unii MW 995 44 A. C1 0 M 98 65 A birun M W99 103 .tan ii C11 W W0 55 A.ip keri M E0 017 9 A.t hica a rm M W 26 6 n A M W9 993 62 A .rov keri on W 9 09 .r 99 13 A ov shan .b sh i 2 5 0 3 A.tu ayto ani A. tu rcic pi rc u s i c us C2 C 5 24 7 C1 7 us C1 c i rs 18 us bu rsic s C u el 1 A. elbu rsic C2 6 . u e 23 31 1 A .elb rcea C 0 5 A ie e 0 ea .p W 00 0 M W0 011 2 A pierc on 80 M W0 929 A. arm iC an M W9 341 A.c ur i M W99 009 .sch ovi C22 sis M 98 61 A urak ne n W a 2 s . d 8 M 9 C2 2 ma 1A MW 9800 2 A.ha thustra 7 AD 0003 A.zara C1 MW 02531 .paulae WE 0127 A apvadi 0 .z W A 19 M 9226 vadi C MW9 374 A.zap 9 9 W lis M hyl 36 A.p AD980 4 A.phyllis MW9917 hyllis C85 MW00348 A.p MW00452 A.phyllis C75 MW98170 A.maraschi C16 MW99057 A.merhaba C17 MW98136 A.w agneri C18 MW9924 0 A.altivag AD980 ans C21 12 A.a ltivagan MW99 s MW9 353 A.altiv agans MW 8313 A.s C22 9 MW 9465 A ertavulen sis C MW 98138 .kanduli 20 A C25 .pos MW 9 8 1 5 MW 9818 4 A.po eidon C s 2 0 eid 0 MW 9 8 0 A. on C2 W 98 97 A pose W E02 203 .ern idon C 1 e E 6 A s 1 2 M 0 .m 9 1 ith ti C1 M W9 245 A. 8 r M W9 807 4 A. senn idate W 81 9 mo an sC 00 8 A. en 23 f s 26 9 A lyc idii so i s iu . 2 rkh A. hop s en kla ffe si s u s ri C sc h u 16 ria ni C5 6 Fig. 48. MrBayes, ITS2, Agrodiaetus, 80% Apart from several small clusters of closely related species only few larger clusters appear with a high level (>90%) of confidence: • menalcas-group (menalcas, alcestis, interjectus, dantchenkoi, aroaniensis, humedasae, fabressei, ainsae, fulgens) • iphigenia-group (iphigenia, baytopi, rovshani, tankeri, iphicarmon, turcicus) • carmon-group (carmon, surakovi, sekercioglui) • antidolus-group (antidolus, kurdistanicus, femininoides, morgani) 55 Chapter 3 The last two groups form a cluster together with the taxa dama, peilei, huberti, theresiae, guezelmavi, arasbarani, elbursicus, ninae, turcicola, pierceae, zarathustra, zapvadi and the undescribed taxon from Ahar with a posterior probability of 81% and the admetus-group (admetus, ripartii, nephohiptamenos, demavendi, lorestanus, khorasanensis) reappears with a confidence level of 84%. Relationships between these groups are unresolved. 100 Polyommatus 76 100 100 59 60 52 100 100 100 67 97 99 Plebeius (Plebejides) pylaon Plebeius (Kretania) eurypilus Plebeius (Vacciniina) alcedo Plebeius (Vacciniina) morgianus Polyommatus (Cyaniris) semiargus 100 Plebeius (Plebeius) argus Plebeius (Aricia) eumedon Plebeius (Plebejidea) loewii Plebeius (Agriades) pyrenaicus Plebeius (Aricia) anteros Plebeius (Aricia) torulensis Plebeius (Aricia) isauricus Plebeius (Aricia) cramera Plebeius (Aricia) artaxerxes Plebeius (Aricia) montensis (Spain) Plebeius (Aricia) montensis (Morocco) Lysandra 100 Tarucus theophrastus Fig. 49. MrBayes, ITS2&COI, Polyommatiti, condensed tree (50% confidence level) 100 97 69 76 66 Lysandra syriaca Lysandra ossmar (Turkey: Isparta) Lysandra corydonius (Turkey: Erzurum) Lysandra ossmar (Turkey: Sivas) Lysandra corydonius (Turkey: Igdir) 30 Lysandra bellargus (Turkey) 76 Lysandra bellargus (Italy) 100 Lysandra bellargus (Iran) Lysandra bellargus (Spain) Lysandra albicans (Spain: Huesca) Lysandra albicans (Spain: Soria) 31 Lysandra albicans (Spain: Huesca) 100 Lysandra coridon (Spain: Burgos) 51 Lysandra coridon (Spain: Barcelona) 84 Lysandra coridon (Italy: Aosta) 98 Lysandra caelestissimus (Spain: Teruel) 94 50000 Fig. 50. MrBayes, ITS2&COI, Lysandra 100 97 100 69 100 98 Lysandra syriaca Lysandra ossmar (Turkey: Isparta) Lysandra corydonius (Turkey: Erzurum) Lysandra ossmar (Turkey: Sivas) Lysandra corydonius (Turkey: Igdir) Lysandra bellargus (Turkey) Lysandra bellargus (Italy) Lysandra bellargus (Iran) Lysandra bellargus (Spain) Lysandra albicans (Spain: Huesca) Lysandra albicans (Spain: Soria) Lysandra albicans (Spain: Huesca) Lysandra coridon (Spain: Burgos) Lysandra coridon (Spain: Barcelona) Lysandra coridon (Italy: Aosta) Lysandra caelestissimus (Spain: Teruel) Fig. 51. MrBayes, ITS2&COI, Lysandra, condensed tree (95% confidence level) In the combined data set of COI and ITS-2, the subgenus Lysandra forms a monophyletic clade at the base of the phylogram while the other Polyommatus (excluding Cyaniris) are found in a highly supported monophyletic clade at the tip of the tree (Fig. 49, Fig. 50, Fig. 56 A molecular phylogeny of Agrodiaetus 51). The sister group to this clade is a weakly supported Plebeius monophylum which includes Cyaniris. Apart from the well supported and resolved Aricia-monophylum (excluding A. eumedon) the relationships within the genus Plebeius remain poorly resolved. Lysandra syriaca appears to be the sister species of all other Lysandra species which are divided into four well-supported clades, but it is conspicuous that corydonius and ossmar do not appear as monophyletic units. The other Polyommatus species split into seven groups (Fig. 52, Fig. 53) and Neolysandra coelestina (which is separated from the other two Neolysandra species) appears as sister taxon to the remaining Polyommatus. The relationships between the other main clades, which appear with a posterior probability of 100%, remain unresolved. 100 Agrodiaetus Polyommatus dorylas (Spain) 28 90 Polyommatus dorylas (NE Turkey) Polyommatus thersites (Iran) Polyommatus thersites (Spain) 100 Polyommatus icarus (Morocco) 100 Polyommatus menelaos 85 Polyommatus eroides eroides 44 100 Polyommatus eroides forsteri 100 Polyommatus icarus (Iran) 62 Polyommatus icarus (Greece) 100 Polyommatus andronicus 100 73 Polyommatus icarus (Spain) 73 Polyommatus escheri 100 Meleageria daphnis brandti 100 Meleageria daphnis versicolor Meleageria daphnis marcida 100 Polyommatus amandus abdelaziz 31 Polyommatus amandus amandus Neolysandra corona 100 Neolysandra fatima 100 Polyommatus aedon aedon 99 Polyommatus cornelia (Adana Prov.) Polyommatus cornelia (Erzincan Prov.) 100 Polyommatus cornelia (Kayseri Prov.) 100 Polyommatus myrrhinus (Erzurum Prov.) 66 98 Polyommatus myrrhinus (Erzurum Prov.) Neolysandra coelestina 100 94 200000 Fig. 52. MrBayes, ITS2&COI, Polyommatus (excl. Lysandra) 100 100 100 100 100 100 94 100 100 100 100 100 100 99 100 100 98 100 Agrodiaetus Polyommatus dorylas (Spain) Polyommatus dorylas (NE Turkey) Polyommatus thersites (Iran) Polyommatus thersites (Spain) Polyommatus icarus (Morocco) Polyommatus menelaos Polyommatus eroides eroides Polyommatus eroides forsteri Polyommatus icarus (Iran) Polyommatus icarus (Greece) Polyommatus andronicus Polyommatus icarus (Spain) Polyommatus escheri Meleageria daphnis bran dti Meleageria daphnis versicolor Meleageria daphnis marcida Polyommatus amandus abdelaziz Polyommatus am andus amandus Neolysandra corona Neolysandra fatima Polyommatus aedon aedon Polyommatus cornelia (Adana Prov.) Polyommatus cornelia (Erzincan Prov.) Polyommatus cornelia (Kayseri Prov.) Polyommatus myrrhinus (Erzurum Prov.) Polyommatus myrrhinus (Erzurum Prov.) Neolysandra coelestina Fig. 53. MrBayes, ITS2&COI, Polyommatus (excl. Lysandra), condensed tree (95%) 57 Chapter 3 36 MW00177 A.gorbunovi C20 40 MW00178 A.gorbunovi 100 MW00129 A.gorbunovi C20 100 WE02674 A.gorbunovi 100 MW00330 A.pseudoxerxes C15 MW00179 A.cyaneus MW99094 A.cyaneus C17 100 40 MW99448 A.cyaneus C18 100 WE02621 A.sennanensis 90 MW98203 A.mithridates C23 94 99 WE02454 421 A.mofidii 100 MW99465 A.kanduli C25 98 MW99353 A.altivagans C22 MW98313 A.sertavulensis C20 MW98136 A.wagneri C18 100 96 MW98170 A.maraschi C16 55 100 MW99240 A.altivagans C21 98 MW99057 A.merhaba C17 AD98012 A.altivagans 100 MW98138 A.poseidon C2 0 71 MW98154 A.poseidon C 21 100 100 100 MW98180 A.poseidon C19 83 MW99501 A.putnami C25 72 MW99408 A.hopfferi C15 49 MW98189 A.hopfferi C16 MW98079 A.lycius MW98097 A.ernesti C18 66 37 MW98285 A.sigberti C25 MW99006 A.firdussii C30 67 MW00234 A.firdussii C25 60 100 AD98009 A.pseudactis 100 MW99058 A.artvinensis C25 95 MW98162 A.actis C17 42 MW00151 A.firdussii 100 MW99247 A.firdussii C24 95 MW99413 A.firdussii C25 37 JC00045 A.nephohiptamenos 80 MW00015 A.demavendi C70 100 MW00185 A.demavendi C70 100 MW99382 A.demavendi C70 37 WE02677 A.demavendi 28 60 JC01014 A.admetus MW01014 A.ripartii C90 52 JC00043 A.ripartii 49 WE02431 A.khorasanensis 49 88 MW99196 A.ripartii C90 MW99407 A.demavendi C60 42 WE02535 A.lorestanus 100 MW00189 A.demavendi 72 MW99105 A.demavendi C66 100 100 WE02491 A.achaemenes WE85001 A.shahrami 100 MW00348 A.phyllis C85 100 MW00452 A.phyllis C75 AD98036 A.phyllis 100 MW99174 A.phyllis 99 52 65 MW00262 A.klausschuriani C56 35 39 WE02451 A.tenhageni WE00002 A.glaucias MW00393 A.erschoffii C14 11 97 MW00101 A.darius MW00347 A.posthumus C85 39 MW00444 A.birunii C11 95 MW00267 A.birunii C10 100 MW00102 A.birunii C11 57 MW00072 A.birunii C11 43 MW00409 A.caeruleus C20 24 MW00547 A.birunii C10 95 28 56 DS00001 A.poseidonides DS01001 A.iphigenides 100 MW98049 A.iphigenia C14 55 78 MW99009 A.iphigenia C12 MW99135 A.turcicus C25 43 MW99203 A.turcicus C24 100 86 MW98103 A.iphicarmon 96 97 MW99372 A.baytopi C27 MW99565 A.tankeri 100 MW00176 A.rovshani 100 WE02662 A.rovshani MW99309 A.baytopi 10 0 MW99559 A.tankeri 39 100 MW00064 A.valiabadi MW00498 A.valiabadi C23 25 JM00001 A.fabressei 41 MW01039 A.fabressei 100 MW01001 A.ainsae 48 100 MW01053 A.ainsae MW01107 A.fulgens 94 100 MW99591 A.humedasae C38 93 100 MW99605 A.humedasae JC00040 A.aroaniensis MW98172 A.menalcas 93 MW98315 A.alcestis C20 100 MW98212 A.alcestis C21 100 MW99164 A.interjectus C31 75 MW00231 A.alcestis C19 93 18 MW99274 A.dantchenkoi C42 53 MW99319 A.dantchenkoi C42 95 22 MW99276 A.dantchenkoi C42 15 MW99471 A.dantch.Xmenalcas C50 MW00539 A.dizinensis C17 MW00269 A.iphidamon C14 100 100 MW00328 A.iphidamon MW99292 A.pierceae C21 97 MW99341 A.pierceae C23 MW00226 A.femininoides C27 96 WE02671 A.femininoides C27 96 MW99393 A.antidolus C42 100 93 MW99406 A.antidolus C44 100 MW99286 A.kurdistanicus C55 WE02591 A.peilei C39 54 100 WE02614 A.morgani C27 100 47 MW98205 A.dama MW98261 A.schuriani C80 AD98001 A.surakovi 100 MW98009 A.carmon 100 MW00032 A.hamadanensis C22 52 MW00051 A.elbursicus C17 90 100 MW00110 A.elbursicus C18 84 MW00316 A.elbursicus C17 38 MW00058 A.elbursicus C18 61 MW00232 A.elbursicus C18 53 100 WE02661 A.arasbarani MW00127 A.paulae C17 WE02531 A.zarathustra C22 100 MW98240 A.theresiae C59 100 39 MW98294 A.guezelmavi MW99479 A.turcicola C20 44 33 MW99508 A.ninae C34 57 AD98018 A.ninae 100 MW99226 A.zapvadi 100 MW99374 A.zapvadi C19 AD98024 A.huberti 78 MW99095 A.huberti C34 100 46 MW99552 A.huberti C33 MW99546 A.damon MW99613 A.damon 100 100 100 100 100 100 99 100 98 100 96 100 98 100 100 100 100 95 100 95 100 100 100 100 100 100 100 99 100 97 95 100 100 100 96 97 100 100 100 100 100 100 100 100 100 100 100 100 97 96 96 100 100 100 100 100 100 100 100 100 100 100 100 100 100 MW00177 A.gorbunovi C20 MW00178 A.gorbunovi MW00129 A.gorbunovi C20 WE02674 A.gorbunovi MW00330 A.pseudoxerxes C15 MW00179 A.cyaneus MW99094 A.cyaneus C17 MW99448 A.cyaneus C18 WE02621 A.sennanensis MW98203 A.mithridates C23 WE02454 421 A.mofidii MW99465 A.kanduli C25 MW99353 A.altivagans C22 MW98313 A.sertavulensis C20 MW98136 A.wagneri C18 MW98170 A.maraschi C16 MW99240 A.altivagans C21 MW99057 A.merhaba C17 AD98012 A.altivagans MW98138 A.poseidon C20 MW98154 A.poseidon C21 MW98180 A.poseidon C19 MW99501 A.putnami C25 MW99408 A.hopfferi C15 MW98189 A.hopfferi C16 MW98079 A.lycius MW98097 A.ernesti C18 MW98285 A.sigberti C25 MW99006 A.firdussii C30 MW00234 A.firdussii C25 AD98009 A.pseudactis MW99058 A.artvinensis C25 MW98162 A.actis C17 MW00151 A.firdussii MW99247 A.firdussii C24 MW99413 A.firdussii C25 JC00045 A.nephohiptamenos MW00015 A.demavendi C70 MW00185 A.demavendi C70 MW99382 A.demavendi C70 WE02677 A.demavendi JC01014 A.admetus MW01014 A.ripartii C90 JC00043 A.ripartii WE02431 A.khorasanensis MW99196 A.ripartii C90 MW99407 A.demavendi C60 WE02535 A.lorestanus MW00189 A.demavendi MW99105 A.demavendi C66 WE02491 A.achaem enes WE85001 A.shahram i MW00348 A.phyllis C85 MW00452 A.phyllis C75 AD98036 A.phyllis MW99174 A.phyllis MW00262 A.klausschuriani C56 WE02451 A.tenhageni WE00002 A.glaucias MW00393 A.erschoffii C14 MW00101 A.darius MW00347 A.posthum us C85 MW00444 A.birunii C11 MW00267 A.birunii C10 MW00102 A.birunii C11 MW00072 A.birunii C11 MW00409 A.caeruleus C20 MW00547 A.birunii C10 DS00001 A.poseidonides DS01001 A.iphigenides MW98049 A.iphigenia C14 MW99009 A.iphigenia C12 MW99135 A.turcicus C25 MW99203 A.turcicus C24 MW98103 A.iphicarm on MW99372 A.baytopi C27 MW99565 A.tankeri MW00176 A.rovshani WE02662 A.rovshani MW99309 A.baytopi MW99559 A.tankeri MW00064 A.valiabadi MW00498 A.valiabadi C23 JM00001 A.fabressei MW01039 A.fabress ei MW01001 A.ainsae MW01053 A.ainsae MW01107 A.fulgens MW99591 A.humedasae C38 MW99605 A.humedasae JC00040 A.aroaniensis MW98172 A.menalcas MW98315 A.alcestis C20 MW98212 A.alcestis C21 MW99164 A.interjectus C31 MW00231 A.alcestis C19 MW99274 A.dantchenkoi C42 MW99319 A.dantchenkoi C42 MW99276 A.dantchenkoi C42 MW99471 A.dantch.Xmenalcas C50 MW00539 A.dizinensis C17 MW00269 A.iphidamon C14 MW00328 A.iphidamon MW99292 A.pierceae C21 MW99341 A.pierceae C23 MW00226 A.femininoides C27 WE02671 A.femininoides C27 MW99393 A.antidolus C42 MW99406 A.antidolus C44 MW99286 A.kurdistanicus C55 WE02591 A.peilei C39 WE02614 A.morgani C27 MW98205 A.dama MW98261 A.schuriani C80 AD98001 A.surakovi MW98009 A.carmon MW00032 A.hamadanensis C22 MW00051 A.elbursicus C17 MW00110 A.elbursicus C18 MW00316 A.elbursicus C17 MW00058 A.elbursicus C18 MW00232 A.elbursicus C18 WE02661 A.arasbarani MW00127 A.paulae C17 WE02531 A.zarathustra C22 MW98240 A.theresiae C59 MW98294 A.guezelmavi MW99479 A.turcicola C20 MW99508 A.ninae C34 AD98018 A.ninae MW99226 A.zapvadi MW99374 A.zapvadi C19 AD98024 A.huberti MW99095 A.huberti C34 MW99552 A.huberti C33 MW99546 A.damon MW99613 A.damon 100000 Fig. 54. MrBayes. ITS2&COI. Agrodiaetus 58 Fig. 55. MrBayes. ITS2&COI. Agrodiaetus, 95% A molecular phylogeny of Agrodiaetus The subgenus Agrodiaetus which represents one of these clades splits into 10 groups which can be named according to the taxonomically oldest species (Fig. 56, Fig. 57). One of them which includes only one species, Agrodiaetus damon, appears to be the sister group to all the others. The complete tree is presented in fig. Fig. 54 and the condensed topology (confidence limit of 95%) in Fig. 544. 100 poseidon-group admetus-group 100 erschoffii-group poseidonides-group 55 iphigenides-group iphigenia-group 43 48 97 dolus-group 95 100iphidamon-group 100 carmon-group 100 damon-group 100 60 28 52 56100 100000 Fig. 56. MrBayes, ITS2&COI, Agrodiaetus 100 poseidon-group admetus-group 100 erschoffii-group 100 poseidonides-group iphigenides-group iphigenia-group 97 dolus-group 100 iphidamon-group 100 carmon-group 100 damon-group 100 95 Fig. 57. MrBayes, ITS2&COI, Agrodiaetus, condensed tree (95% confidence limit) 0 100 98 100 96 100 88 73 Apodemia mormo (Riodinidae) MW00497 Favonius quercus (Theclini) MW99045 Maculinea arion (Glaucopsychiti) JC00057 Aricia artaxerxes MW00302 Polyommatus thersites MW00032 Agrodiaetus hamadanensis MW00056 Agrodiaetus elbursicus MW99406 Agrodiaetus antidolus MW00072 Agrodiaetus birunii MW98154 Agrodiaetus poseidon MW00076 Meleageria daphnis MW99014 Polyommatus dorylas MW00412 Polyommatus icarus MW00530 Polyommatus eroides MW00409 Agrodiaetus caeruleus MW99550 Polyommatus myrrhinus MW98228 Lysandra syriaca MW99068 Agrodiaetus ripartii MW00189 Agrodiaetus demavendi MW99105 Agrodiaetus demavendi MW98172 Agrodiaetus menalcas MW99471 A.dantchenkoiXmenalcas MW00231 Agrodiaetus alcestis MW99164 Agrodiaetus interjectus MW00328 Agrodiaetus iphidamon MW99135 Agrodiaetus turcicus Fig. 58. MrBayes, ND1, Lycaenidae, condensed tree (70% confidence limit) For the ND1 analysis most taxa were chosen to represent the main Agrodiaetus clades which were obtained from the combined COI- and ITS2-analyses in order to check if this gene gives a better resolution of clade relationships. The tree obtained from the ND1 analysis (Fig. 58) has little resolution but is compatible with those of the COI- and ITS2-analysis. A 59 Chapter 3 combination of the ND1 dataset with the COI- and ITS2-datasets also does not improve the resolution regarding the relationships of the different Agrodiaetus clades with each other. The tree obtained from the Cytb analysis (Fig. 59) is also compatible with the results from the other genes but due to the low number of taxa sequenced does not provide additional information. 100 Ostrinia nubilalis AF442957 Lycaena thersamon MW98006 Polyommatus cornelia MW98225 Agrodiaetus iphicarmon MW98102 Agrodiaetus admetus MW98055 100 Agrodiaetus turcicola MW99258 51 0.05 Fig. 59. MrBayes, Cytb Maximum parsimony analysis The Maximum parsimony analysis yielded 100000 trees in each of the COI-, ITS2- and the combined analysis because the maximum limit of 100 trees was reached in each replicate. The trees obtained from the COI analysis had scores between 2414 and 2433. The consensus tree calculated from the 300 shortest trees (score=2414) is shown in Fig. 60 for the outgroup & Fig. 61 for Agrodiaetus and that calculated from all trees in Fig. 62 (outgroup) & Fig. 63 (Agrodiaetus). Scores of the ITS-2 analysis were between 1105 and 1107 (39800 of which had the lowest score of 1105). The consensus tree of all trees is shown in Fig. 64. Scores of the combined analysis were between 2599 and 2606. A consensus tree of the 2700 shortest trees is presented in Fig. 65 and the consensus tree of all trees is shown in Fig. 66 & Fig. 67. Bootstrap trees for the three analyses are shown in Fig. 68 (COI outgroups), Fig. 69 (COI Agrodiaetus), Fig. 70 (ITS-2), Fig. 71 (ITS-2 & COI combined, outgroups) and Fig. 72 (ITS-2 & COI combined, Agrodiaetus). MP-trees calculated from the ND1-gene were poorly resolved and different combinations with the COI- and/or ITS-2 gene also did not provide new results and are therefore not presented here. 60 aon a ch io m a tullia apil e h mp em 06 P y h t 440 elii non ury AF0 Coe ias e tham rius l 60 s i li Co 708 .fe d a i us 007 114 I s po alir lii AF 1 044 od m e o 01 de p AF . I icli ha rm MW Iph 001 feist mo sna y 73 I. 02 ia 08 JC 024 em Z.kn 17 AF 02 pod 21 MW 3 A 020 6 08 MW 17 AF JC 00 W 02 055 W 0 A 3 0 MW 104 3 A .art .m ax 8 9 MW A e o 9 99 097 .mo nte rxe MW 001 A.is nte nsis s n a A 0 s MW 1061 .toru uricu is 982 A.c len s s WE 70 A rame is .a MW 000 r 990 03 ntero a 13 N.d s N i . a c MW 003 oelest na 26 ina MW9 8264 P.aedo n P.co MW9 rne 9038 P.corn lia MW982 e li a 35 P.co rnelia MW99538 P.myrrhin us MW99550 P.myrr hinus MW99537 P.myrrhinus M M us ar s u ic us P. icar nic 25 P . d r o 0 n 3 01 3 P.a arus s W 81 c M W9 061 P.i icaru M 00 063 P. tima JC 00 412 N.fa a tim JC W00 301 .fa M 99 69 N orona W c 4 . us M 99 4N and MW 0050 P.am a id 1 rc MW 0200 .ma MW 0290 M aphnis 0 d MW 8274 M. phnis MW9 029 M.da 8 9 phnis a MW .d 076 M da MW00 ar 7 M.m ci MW0008 mandus P.a 7 04 99 MW MW98228 L.syriaca MW99042 L.corydonius MW99536 L.corydon ius MW98129 L.ossmar MW004 6 MW98 9 L.bellargus 2 MW9 78 L.bellarg 9435 us MW L.bell 9 argu MW 9608 L s .bell MW 01011 argu s MW 9914 L.bella rgu MW 995 0 L.co s MW 981 14 L.c rydon ius MW 01 85 L oryd on M 01 092 .oss ma ius M W0 05 L.a O W0 103 9 L. lbica r K9 10 4 a n 90 18 L.a lbica s ns l bi 01 L . L. cor can s g e id nn on ar ge nt i on rid n co do us L. ori on 3 sim .c id 10 2 L .cor stis us le c L 01 1 ae nai W 96 6 M W9 111 L.c yre on M W0 022 A.p med M K96 018 A.eu s u O 99 3 4 arg tophi MW 991 16 P. ris MW 001 4 P.ch ii MW 9912 P.loew gus r MW 98220 .semia MW 0525 C emiargus 0 MW 034 C.s ianus 2 MW0 517 V.morg 0 MW0 lcedo 024 V.a MW00 V.alcedo MW99430 on MW99163 P.pyla MW99303 K.eurypilus M W 0 M W 102 W 004 3 S 0 97 .p MW 2 0 00 07 F.q irith 0 L u o 1 MW MW9 7 L .alc erc us 82 .alc iph us 99 MW 515 30 L iph ron L.v .tit ron 00 y 1 ir MW 19 L. gaur rus MW 995 can eae 980 de 20 n 94 L.th L.the s MW t 000 44 L ersam is on MW0 .asa binu 2009 s L.ph MW9 laea 9398 s S.my r MW01 ta 027 S.e le sculi MW9915 8 S.hyrca nicum MW98002 C.os iris MW01120 C.marshalli C.argiolus MW99084 lus MW02008 C.argio phrastus eo th T. 5 MW0202 dymion 21 T.en MW992 .arion 5 4 0 M ama MW99 icr .v P us 9080 rrag s MW9 u ence P.ab .boetic us 1 3 2L tic 020 MW 102 L.boe icus 0 MW 65 .boet lus 982 L hy MW 2028 .troc rdino 0 a C tis MW 425 bern ges es 99 tes A.a erx is W t o M 62 tax s s hil up 000 .ar .age esti E A JC 57 0 A .ag 64 08 2 0 A 17 00 00 8 AF JC W0 902 9 M W M M MW01019 P.dorylas MW98128 P. dorylas MW9901 4 P.dory JC000 las 3 MW0 9 P.escheri 1009 MW P.esc 0 heri MW 1083 P .ther MW 99128 sites P 0 . 0 t hers MW 302 MW 9800 P.the ites rsit MW 020 8 P.t es MW 00 06 P hersi 5 t es .ica JC 9 9 30 JC 00 57 P.e rus JC 00 042 9 P. roide s 00 02 P. ero 05 9 P ero s yi forst l di eri id . 1 za P . m en es e m en elao el ao s s A molecular phylogeny of Agrodiaetus Fig. 60. MP Majority Rule Consensus Tree of the shortest trees (COI) excluding Agrodiaetus 61 on .dam on 13 AA.damC70 i i 996 MW 99546avend venddi a MW .dem .dem avenndi A e 0 82 81 A dem av C7 993 93 7 A. em di 70 MW MW9 267 6 A.davenndi CC70s 0 8 e i i WE 001 .demmav end ensnos s v n MW 85 AA.de ma asa ame enortii i e r 1 00 183 A.d ho hipt tam ripa arti tii MW 00 015 A.k pho hip A. .rip par MW 00 431 ne ho 05 A .ri 2 A. nep 011 043 2 A MW 0 . 6 E W 004 5 A MW 00 107 0 04 JC 0 W JC 00 M JC M 15 0 C 2 ri C 2 1 ffe idon n C 5 p 2 ho se ido i C 25 A. o e 8 A.p os nam i C C19 40 38 A.p.put tnam on C19 9 9 1 4 A u id n W 98 15 1 .p se do M W 98 906 1 A .po osei M W 9 5 0 3 A . p i us 8 M W 99 18 A lyc s M W 98 180 A. yciu ti C1 M W 98 79 A.l nes ii M W 980 89 .er uss C30 M W 80 7 A .fird ssii 25 M W9 809 5 A irdu ii C M W9 012 A.f duss C25 M W0 006 A.fir erti M W99 234 .sigb actis 5 M W00 85 A seud nsis C2 M W982 9 A.p rtvine M 9800 8 A.a s C17 AD 9905 A.acti rti MW 98162 A.sigbe C25 MW 98284 .firdussii MW 99422 A dussii C25 MW 99413 A.fir i MW 151 A.firdussi C24 MW00 247 A.firdussii MW99 1 A.achaemenes 49 02 WE mi WE85001 A.shahra MW00327 A.erschoffii MW00393 A.erschoffii C14 WE02451 A.tenhageni MW99174 A.phyllis MW99187 AD98036 A.phyllis A.phyllis MW00 MW0 140 A.phylli MW000348 A.phyl s MW 452 A.p lis C85 hyllis MW 00259 C MW 00262 AA.klaussc 75 MW 00335 .klaus huriani C5 WE 0040 A.cae schuri ani C 7 rule M 000 9 A. 56 M W00 02 A caeru us C2 MWW003347 A .glauc leus C 0 20 M 0 58 .po ias MWW000101 A.po sthum us C M 0 44 A. sth M W 02 4 A dar um 85 M W 005 67 .bi ius us MWW0 004 47 AA.bir runii M 0 01 76 .b uni C11 W i i D 0 0 A r D S 0 06 2 .bi uni C1 M S 00 00 0 A.b run i C 0 M W 000 00172 A A.b irun ii C 10 W 99 0 A .b iru ii 11 99 1 5 A .p iru nii C1 52 35 .d os ni C1 1 6 A. ag eid i C1 1 A. tu m on 1 tu rci ar id rc cu a es ic s us C 25 4 C2 us ic ni rc ha i tu vs an A. ro sh i 3 A. ov top i 20 6 A.r bay ker 99 17 2 A. an eri on W 00 66 9 .t nk rm M W 02 930 59 A A.ta hica pi 27 M E 9 5 5 .ip yto i C 9 W 6 A p a W M W9 995 103 A.b ayto nia C15 M W 98 44 A.b ige ia 12 M W 993 72 .iph igen C h M 3 A nia 12 MWW99 029 6 A.ip hige nia C 4 M D98 810 A.ip hige ia C1 A W9 009 A.ip igen M 99 70 iph MWW991049 A. .ainsae s M W98 53 A ulgen M W010 7 A.f sae M 0110 A.ain MW 01078 A.ainsae ei MW 01001 .fabress MW 39 A 0 ssei 1 MW00001 A.fabre badi JM0 4 A.valia badi C23 MW0006 8 A.valia MW0049 1 A.humedasae C38 MW9959 A.humedasae MW99605 JC00040 A.aroaniensis MW98020 A.menalcas MW98172 A.menalcas MW99494 A.menalcas MW98315 MW9821 A.alcestis C20 MW99 2 A.alcestis C21 MW9 164 A.interject us C31 MW 9471 A.da MW 99320 A.d ntch.Xmenalc as C50 MW 99319 A antchen MW 99276 .dantche koi C41 nkoi C M 9927 A.dan W MW 993804 A.dan tchenkoi 42 tch C4 00 A MW 002229 A. .alcesti enkoi C 2 sC 42 alc 31 A .alc estis C 19 estis 19 C19 M M W9 A W W 99 955 D9 99 0 2 80 M M 37 53 A 1 MW W9 W 4 A A. .hu 8 A 99 947 992 .za hub bert .nin MW M 31 6 A 26 pv er i C a e W t MW 002 00 4 A. .zap A.z adi i C3 33 3 t a MW 004 69 A 28 urci vad pvaC19 6 c A 2 i . 00 4 A iph .ip ola C di WE 539 A .iph idamhida C219 i DS 9800 .dizindam on Cmon0 010 1 A en on 1 01 .iph sis C1 4 A.ip ige C1 4 hig nid 7 enid es es M W M 01 W 0 JC 98 14 M 010 084 A.r M M W 1 A ip MW M W9 W 992 4 A .adarti 9 W 99 99 19 992 64 .ad mei C9 6 MW407 068 A. 63 A A.ri met tus 0 r A p u W 991 .de A.ri ipar .rip art s MW E025 02 Amav part tii Cartii ii 35 .de en ii C 90 0 M 0 MW W9 18 A. m di C 90 991 914 9 A lore ave 60 .d s 05 n M A 1 A em tan di MW W991 .dem .demaaven us ave ve di MW 0 9 4 9 3 992 86 A 76 A.a A.demndi C6ndi MW .kur ntido ave 6 9 d n MW9 9393 A.aistaniculus C30di 94 s n MW9 9473 06 A.an tidolus CC55 A.kurd tidolu 4 2 WE02 istanic s C44 61 u WE026 2 A.karindu s C65 s C66 14 A WE02591.morgani C27 A.peilei C MW00226 39 A.feminino WE02671 A.femin ides C27 inoides C2 MW98205 A.dama7 WE02676 A.hamadanensis C21 MW00032 A.hamadanensis C22 MW00001 A.hamadanensis riani C80 MW98261 A.schuceae C23 A.pier MW99341 pierceae C21 A. 18 2 MW9929 A.elbursicus C 8 u s C1 10 MW001 2 A.elbursic sbarani ra e 23 MW00 02661 A.a7 A.paula22 WE aC 7 015 MW0 arathustrlae C1 7 1 u .z a C A .p s 2531 27 A sicu icus WE0 W001 .elbur elburs C18 M 51 A . s 17 u A 056 ursic us C 16 000 MW MW00 A.elb ursic us C on lb sic rm 6 8 005 6 A.e lbur A.ca ui C479 0 MW 0031 9 A.e 009 iogl on C ovi i 8 erc rm rak av 5 W 9 0 M 00 9 k MW .se A.ca A.su zelm C5 34 MW 9 A 60 01 gue iae e C 20 4 8 92 990 980 A. res ina a C C3 ti 9 l r 4 e MW MW AD 829 A.th A.ncico erti be 9 0 08 ur ub .hu t MW 824 995 A. A.h 4 A 9 W W 79 5 2 M M 994 909 980 W 9 D M MW A MW00179 A.cyaneus MW00330 A.pseudoxerxes C15 MW99059 A.m MW99094 A.c erhaba C17 yaneus C17 MW99 MW99 448 A.cyaneus WE026449 A.cyaneus C18 2 1 A.s MW MW 99465 A ennanensi MW 99357 .damocle s WE000178 AA.altivaga s kanduli C ns 25 .gorb MW 267 MW 0012 5 A.gor unovi bun 00 9 A MW 993177 A .gorbun ovi C19 MW ovi M 98 53 .gor M W9 139 A.alt buno C20 M W9 831 A.w ivag vi C2 MWW9981363 A.s agne ans C 0 22 ri M 2 A er M W 991 40 .w tavu C19 W W 98 65 A.a agn len W E0 982 170 A.a ltiva eri C sis C g l E 2 20 0 A t 1 M W W 02 32 3 A .m iva ans C 8 A E 9 4 1 .m ar ga M D9 02 905 21 A A.ps ith asch ns C 21 W 8 53 7 .m e rid i C 25 98 01 6 A. o ud ate 16 18 2 A A.d me fidi oxe s C rxe 23 9 .a am rha i A. lti a b s ho va lis a C pf ga 17 f e ns ri C 16 Chapter 3 Fig. 61. MP Majority Rule Consensus Tree of the shortest trees (COI) of Agrodiaetus 62 aon ach tullia io m apil pha me 06 P onym urythe elii n m s 440 AF0 0 Coe lias e istha liriu s o 6 a iu e 708 07 C 14 I.f pod alir elii 0 d m s 1 AF1 044 W01 clide I.po tha rmo le AF M Iphi 001 feis mo rta y . 73 C02 24 I mia .m 08 J 20 d e 8 S 17 0 po 39 AF W M 3 A 99 6 W 08 M 17 AF M W ri he ida c es arc nis P. h 9 M.m ap hnis 0 d p . 0 0 is 01 29 4 M .da hn W 00 27 9 M .dap cida M W 98 02 M ar tes M W 98 76 i .m M W 00 7 M thers es 0 8 . M W 00 sit P r e M W0 083 P.th tes M W01 128 .thersi s M W99 02 P ersite M 003 8 P.th na MW 9800 N.coro MW 00504 atima MW 99301 N.f ma MW 9469 N.fati MW9 01 P.amandus MW020 amandus MW99047 P. riaca L.sy MW98228 MW99042 L.corydonius MW99536 L.corydon ius MW98129 L.ossmar MW004 MW9 69 L.bellargu s MW9 8278 L.bell MW 9435 L.b argus 9 ellarg MW 9608 us MW 0101 L.bell MW 991 1 L.be argus 4 l larg M 99 0 L us M W9 514 .cor MWW01 8185 L.co ydoniu s M 0 09 L.o rydo ni ss M W 10 2 O W0 010 59 L.alb mar us K 9 1 34 L. ic 90 01 L. albi ans 01 8 L alb can L. .co ica s ge rid ns nn on ar ge nt i M M W9 W 9 1 M W 994 63 MW MW 000 30 P. V p MW 005 982 24 V .al yla 1 c o 2 MW 0203 7 V. 0 P .alce edo n 005 4 C mo .loe do r 25 .se gia wi C.s mia nu i em rg s iar us gus on rid on us co id L. or on im 3 .c rid tiss 10 2 L .co les tina 01 61 L ae eles W 9 1 6 .c M W9 011 22 L N.co na ia M W 0 3 M K96 901 3 N.d edon a O W9 00 P.a rneli 6 o M 00 2 c . ia WEW003 64 P cornel M 982 5 P. nelia MW 9823 8 P.cor inus MW 9903 P.myrrh MW 538 rhinus r 9 y MW9 9537 P.m hinus MW9 550 P.myrr ino s bernard MW99 Euphilote AF170864 teros MW98270 A.an MW01061 A.cramera MW99001 A.torulensis MW99097 A.isa uricus MW0104 MW02 8 A.montensis JC00 033 A.monte nsis MW 055 A.arta xerxe JC 00020 s JC 00062 A.agest MW00057 A.ages is ti s 9 90 A.ar t 2 a 8 A.a xerxe s ges ti s M 99 W 0 M 158 10 2 MW W 02 S.h 7 S MW 010 02 yrc .e MW 00 23 1 Z. ani scu S 4 k c MW 0200 97 F .piri nys um li 7 L .qu tho na 00 MW 017 .al er us c c MW 0200 L.alc iphr us 000 9 L. iph on r p 44 L. hla on M MW9 W9823 asabineas 0 L. 9515 u tityr s L.v MW0 u 0119 irgaurea s L.can e MW98 dens 094 L.t he MW9952 rsamon 0 L.th MW98002 C.osetis iris MW99221 T.endymion MW99045 M.arion ma MW99080 P.vicra s erragu nc be .a P stus MW02031 eophra 25 T.th .argiolus MW020 C 4 08 iolus MW99 08 C.arg halli s 0 r 2 a MW0 20 C.m oeticuss 1 b ticu 1 . 0 L MW 8265 .boe icus t 9 L s MW 2028 L.boe hylu s c icu i 0 2 o r W 2 t . a h M 010 5 C ren top s MW 942 .py hris rgu on A 9 c .a d s . MW 9018 4 P 6 P me pilu 2 11 eu ry 9 1 MW W99 00 A. .eu W M M 134 3 K 99 30 W 99 M W M MW01019 P.dorylas MW98128 P. dorylas MW990 MW02 14 P.dorylas 0 0 6 P.ica MW rus MW 00530 P JC0 99579 .eroides f P orste 0 . eros JC 029 ri JC 00051 P.men yildiza e 0 e MW 004 P.m laos ene JC 0 2 P M 00 102 .ero laos JC W9 061 5 P.i ides ca M 0 81 P JC W0 006 33 P .and rus 00 04 3 P .ic roni 03 12 .ic aru cus 9 P. aru s P. ic s es aru ch s er i A molecular phylogeny of Agrodiaetus Fig. 62. MP Majority Rule Consensus Tree (COI) excl. Agrodiaetus 63 p grou n Out damoon A. 13 .dam rtii 996 6 A ripa rtii MW 9954 05 A. .ripa 90 MW 011 72 Aartii Cartii ii MW 010 .rip A.rippart 0 MW 14 A264 A.ri i C690 d 0 01 99 263 ven tii CC90di MW MWW99 ma ripar rtii en us i M .de A. ripa mavtan nd 6 7 A 96 A. e s ve 6 40 91 8 .d re a i C 99 9 906 02 A A.lodemend W MW M W9 91 35 A. av M W9 25 41 m M E0 91 .de W W9 5 A M 10 99 W M M W E0 MW 99 0 1 2 M W WE 431 018 04 99 0 A 9 A. M 382 267 .kh A.d de MW M W99 A. 7 A ora emma W MW 00 00 381dem .de san av ven e 1 MW 001 85 186 A.d ave mav ens nd di 00 83 A.de A.d em ndi en is i 01 A. m e a C di 5 A de av ma ve 7 MW .d ma en ve nd 0 e JC0 i v J 98 m en di nd JC0 0046 C010084 Aaven di CC70 i 004 A.n 14 .ad di C 70 5 A eph A.a me 70 .ne ohip dm tu p MW JC hoh tam etuss 0 MW 0539 A 00043iptameenos 0042 . n 4 A.i dizinenA.ripar os M t p s MW0 W00328 hidamois C17ii 026 nC A.i MW99 9 A.iphidaphidamo14 203 A mon n MW9 .turcicus C14 MW9913 9526 A.turcicC24 5 A.turcic us us C25 MW9956 MW99372 A.b5 A.tankeri aytopi C27 MW99344 A.baytop i MW98103 A.iphicarmon MW99559 A.tankeri topi A.bay 309 MW99 ani WE02662 A.rovsh rovshani MW00176 A.higenides ip A. WE98001 A.iphigenides ara DS01001005 A.dagmides DS00 .poseidonhrami A a 1 h 0 .s es 0 DS00 85001 Ahaemeneni WE 1 A.ac enhag 14 .t 9 C A ii 24 ii WE0 E02451 rschoffschoff56 W 3 A.e A.er ni C 57 039 0327 churia ni C us 0 MW MW0 uss huria hum 85 t la c C A.k lauss A.posmus cias0 62 u 2 k 002 9 A. 358 sthu .gla C 0 MW 0025 W00 A.po 02 A leus s C2ius 1 M 47 00 eru leu dar 1 0 MW 0 a u . C 3 00 WE A.c aer 1 A unii ii C1 10 MW 09 .c 10 ir n C 11 04 35 A 00 A.b biru unii ii C 11 0 C 3 W 4 . r MW 00 M 044 7 A .bi irun nii 0 26 7 A .b iru MW A 0 MWW0 054 76 A.b M W0 04 02 M W0 001 M W M Fig. 63. MP Majority Rule Consensus Tree (COI) of Agrodiaetus 64 7 C2 es id o in 9 7 in 3 2 6 m iC iC 6 fe ile an C 80 A. .pe org dus ni C 21 71 A .m rin ria e C 3 26 591 4 A .ka chu ea C2 17 E0 02 261 12 A A.s ierc ceae us C W E 0 6 61 A.p ier sic s W E 02 2 2 .p ur icu C18 W E 98 29 1 A .elb rs us 16 W W 99 34 A lbu sic M W 99 51 A.e lbur icus C 7 M W 000 56 .e urs M W 00 58 A .elb ae C1 M W0 00 9 A aul e 22 M W0 005 A.p aula stra C M W0 127 A.p athu i M W00 157 .zar aran 18 M W00 31 A .arasb icus C M E025 61 A lburs C17 W E026 2 A.e ursicus 18 W 0023 6 A.elb rsicus C W M 0031 A.elbu ctis MW 00110 .pseuda MW 8009 A irdussii AD900125 A.f ussii C30 MW 006 A.fird 25 MW99 234 A.firdussii C MW00 5 A.sigberti C25 MW9828 A.artvinensis C25 MW99058 MW00151 A.firdussii MW99247 A.firdussii C24 MW99413 A.firdussii C25 MW99422 A.firdussii C25 MW98162 A.actis C17 MW9828 MW98 4 A.sigberti MW9 097 A.ernes MW988079 A.lyciu ti C18 s MW 089 A.l MW 98138 A ycius MW 98189 A .poseidon 9 .h C20 MW 940 op MW 981548 A.hop fferi C16 ffer 99 A MW 995061 A. .poseid i C15 MW M 98 01 putn on C2 1 A W9 180 A.pu ami M D98 8183 A.p tnam C25 MWW00 012 A.pooseido i C25 W 00 129 A.a sei n C ltiv do 1 M E 1 M W 026 77 A.g ag n C 9 M W 00 75 A. orb ans 19 M W9 994 178 A.g gorb unov M W i 6 A o u M W 0 93 5 .g rbu no C2 M W 00 017 57 A A.da orbu nov vi C2 0 W 9 9 33 9 . a m n i C 0 99 05 0 A. lti oc ovi 19 09 9 A. cya vag les 4 A. pse ne an ka A. me u us s nd cy r h d o uli an ab xe C2 eu a rxe 5 s C1 s C C 7 15 17 18 C s eu s sis 22 an eu en C cy an an ns 8 0 A. cy n a C1 8 A. en vag ri 19 C2 44 9 .s lti ne i C sis r A a g 4 99 4 1 A. a ne len 16 W 99 62 3 .w ag vu i C 23 M W 02 935 36 A A.w erta sch tes C 5 2 M E 9 1 9 . s a ra d a W W 98 13 3 A .m hri ns C 21 a M W 8 1 A it C M W9 983 170 A.m ltivaggans xes M W 98 03 A.a iva xer 2 5 lt o M MWW98916 0 A.apseud ii 7 M W9 924 A. ofid ba C1 M W9 321 A.m rha M 02 21 .me lis WEE024057 A .dama W W99 36 A M E025 W M M W M W0 000 W M 0 00 72 W 0 6 A 00 4 5 0 . M 34 2 A A.b biru MWW0 8 A .ph irun nii MW 9 014 .ph ylli ii C C1 MW 9 y s 00 AD 99 187 0 A. llis C7 11 1 1 MW498 980 74 A.p phy C85 5 3 A A h lli 6 . 0 M 006 vali A. .phy yllis s MWW01 4 A. abadphyl llis 0 l v 01 78 a i C is MWMW01 107 A.a liaba 23 010 053 A.fu insa di 39 A. lge e M JM W010 A.fab ainsans MW 00001 01 A.aresseie 994 A .f MW9 94 A abreinsae .me ssei MW9 8172 A.m nalc MW9 8020 A.m enalc as 8315 enalc as A M .a W lc 982 estis C as MW9947 20 1 A.dan 12 A.alcestis MW9932 tch.Xmenalcas C21 0 A. C50 MW99319 A.ddantchenkoi C41 antchenkoi C4 MW99276 A.dantc 2 henkoi C42 MW99274 A.dantchenkoi C42 MW99164 A.interjectus C31 MW99380 A.alcestis C19 is C19 MW00231 A.alcest lcestis C19 MW00229 A.ahumedasae A. MW99605 medasae C38 1 A.hu niensis MW9959 0040 A.aroa nia C14 JC0 .iphigeenia C122 A 9 4 0 1 ig MW98 170 A.iph igenia CC15 h 9 MW9 009 A.ip higenia enia ig p 9 .i h 9 A p W .i 6 M 29 A 9810 MW AD980 W AD98001 A.surakovi MW99060 A.carmon C79 MW99289 A.sekerci oglui C46 MW9800 AD9801 9 A.carmon MW99058 A.ninae 3 A.hub MW MW 99552 A.h erti C36 MW 99226 A uberti C33 MW999314 A.t.zapvadi urcic MW 947 AD 9937 6 A.zap ola C20 MW98024 4 A.zap vadi C1 9 vad M 990 A.h M W99 95 A uber i C19 MWW995479 A .hube ti rti C M 9 08 .tu M W9 824 A.n rcico 34 M W0 829 0 A. inae la C W W00000 4 A. there C34 20 gu si M E 0 1 M W 026 32 A.h ez ae M W 98 76 A. am elm C59 M W9 002 205 A.h ham ada avi n W M a a 2 M W 9 92 6 A.d m da en M W 99 937 86 A A.fe am ada nen sis W 99 39 6 .k m a ne sis ns 99 4 3 A. ur ini i s C2 2 47 06 A. an dis noi C2 3 A. an tido tan des 1 A. an tid lu ic C ku tid olu s us 27 rd ol s C3 C5 i s u s C4 0 5 ta C 2 ni 4 cu 4 s C 65 Chapter 3 us ra s t s oph rgu .the mia gus 25 T .se iar ilus 020 34 C sem ryp on MW 020 5 C. .eu pyla us MW 0052 303 K63 P.enaicdons MW W99 991 .pyr umeianu do M MW 8 A A.e rg lce tis o a s a 01 4 99 913 V.m4 V. age er tis MW W9 517 002 3 A. cramges xes s M 00 0 3 A. .a er cu is 0 x i W MW M 02 061 48 A rta aur ens MW 01 10 A.a .is rul A o 0 MW W 055 97 A.t M 0 0 1 0 9 0 JC W9 90 M W9 M 47 1 A M M .da W9 M W9 ntc 81 W 83 h. 7 98 1 X m 2 A 5 M 212 A. en .m W a a e M M W W 0 1 A. a l c e l c a n a l 0 0 10 c e st s l c M M 10 10 7 s is C as MW W99 JM W0 39 53 AA.futis CC2 50 0 MW 9927 164 0000 1001A.fa .ainlgen21 b MW 9927 4 A. A.int 1 A.f A.a res sae s d e JC0 9931 6 A.d antc rjec abreinsasei 004 9 A ant hen tus sse e 5 A .dan che ko C3 i .ne i ph tche nko C4 1 WE 024 JC0 ohiptankoi i C422 31 A 004 me C4 3 .k n JC0 hora A.rip os 2 MW0 10 s a 0 MW9 015 A.d 14 A.aadnensisrtii em 9407 metu s MW00 A.demaavendi C vendi 70 498 A .valia C6 0 M a di C MW0018 W00064 A.vbal 2 5 A.de iabad3i WE02535 mavendi C70 A. restanu MW99196 A.rloipa s rtii C90 MW99105 A.demavendi C66 MW00189 A.demavendi MW01014 A.ripartii C90 i C70 MW99382 A.demavend avendi WE02677 A.dem M W 99 2 C2 is 2 s en C2 an ra ad ust 17 m C h t 9 ha ra e i A. a ula d C1 2 A.z .pa pva adi 3 a pv 0 1 A 5 z . 00 53 27 A za llis C7 W 2 1 6 A. hy lis 5 M E0 00 922 74 A.p phyl lis W W 9 93 74 A. yl is C8 25 M W 9 1 2 .ph yll ii C 4 M W 99 45 A .ph uss C2 25 M W 00 36 A ird sii C M W 80 348 A.f rdus nsis M D9 00 13 .fi vine 7 A W 94 47 A .art C1 25 M W9 92 8 A ctis ii C M W9 905 A.a duss tis M W9 162 A.fir dac M W98 234 .pseu sii M W00 09 A .firdus i C25 M 980 51 A igbert 23 ADW001 5 A.s hridates C M W9828 3 A.mit nensis M 9820 A.senna 14 C n MW02621 .iphidamo WE 00269 A hidamon MW 00328 A.ip 7 C1 s si MW 539 A.dizinen MW00 177 A.gorbunovi C20 MW00 i nov MW00178 A.gorbu MW00129 A.gorbunovi C20 WE02674 A.gorbunovi MW00330 A.pseudoxerxes C15 MW00179 A.cyaneus MW9909 MW994484 A.cyaneus C17 A.cyan MW 00 6 A.firduseus C18 WE099 sii 454 A.m MW92 ofidii C30 MW 9465 MW 99353 A.kandu MW 99240 A.altivag li C25 AD9 99057 A.altiv ans C2 ag 2 80 A MW 98 12 A .merh ans C2 MW 9 136 .altiva aba C 1 MW DS 9883170 AA.wag gans 17 D 0 . m ne 1 M S0 000 3 A ara ri C18 M W 100 1 A .ser sch MWW00054 1 A. .pose tavule i C16 0 7 ip id M n M W 00 44 A hig on sis M W 0 39 4 A .bir en ide C20 M W 0 0 4 3 . b un i d e s M W 00 010 09 AA.er iruni ii C1 s 0 s i M W 0 2 2 M W 00 007 67 A. .cae cho C11 W 00 1 2 A. bir ru ffii 99 3 01 A bir un leu C1 . 4 i s u i 56 7 A. bir n C C 4 5 A. da un ii C 11 20 A. po riu ii C 10 ta st s 1 1 nk hu er m i us C8 5 25 4 i er s C 2 2 nk cu s C C1 ta rci u ia A. tu cic n on 4 9 A. tur ige rm C1 55 35 A. iph ica nia 6 C5 99 1 03 A. iph ige ni ni W 9 9 2 9 A . h ha i M W 99 900 03 A.iprovs han huria M W 9 81 49 A. vs sc M W 9 8 0 6 . ro u s i 7 A la p M MWW9001662 A.kbayto s M W 02 262 A. ciu i C18 19 M E 00 09 .ly est n C A rn 3 o W 5 MWW9980797 A.eposeidmi C2 0 M W9 809 A. utna n C2 M W9 8180 A.p eido C21 1 M 9 os 0 on MW 995138 A.p.poseid i C16 MW 98 154 A .hopffer C15 MW A 98 eri MW 8189 .hopff C27 MW999408 AA.baytopi MW 9372 ucias MW90002 A.glanhageni WE002 451 A.te enes WE 24 A.achaemmi WE0 91 shahra WE85001 A. M M W M W 98 M M W9 981 27 MWW9 W9 81 85 0 A 9 951 82 29 L. .an M 953 4 L 28 L.o oss te MWW01 6 L. .co L.s ssmmaros M 0 03 co ryd yr a r MWW01 101 4 L rydo oniiaca r 0 8 .c u OK 960 MW010959 L L.cooridnius s 22 01 2 L .alb rid on 1 L MW .cae 16 L.albicicanon MW 99 lest .co an s 6 MW 01011 12 L.issimridons MW 98278 L.bellcoriduos MW 99608 LL.bellaargusn 004 .be rgu MW069 L.bellllarguss 0116 argu M MW99 W98220 P.arguss P.l 01 MW9803 N.coelesotiewii a MW000729 M.daphnnis MW002906 M.daphnis M.marcida JC00063 P.ic s JC00061 P.andronaru icus MW01025 P.icarus JC00042 P.eroides s JC00029 P.menelao es forsteris MW00530 P.eroid icaru P. 2 41 00 MW 6 P.icarus MW0200 P.dorylass 14 MW990019 P.doryitlaes 1 MW0 83 P.thers es 10 hersit a MW0 0302 P.t N.fatimna MW0W99301 N.corodus M 0504 aman dus 0 MW 047 P. .amanrneliaia P co e l 99 MW 02001 38 P. .cornnelias MW W990235 PP.corrhinuus M 98 64 myr rhin on MW 982 0 P. .myr .aedheri n MW 955 7 P 6 P esc mo on 9 3 32 . a e MW 995 00 39 P A.d.damasa 38 MW MW000 613 6 A ed e C sis 9 JC W99 954 hum sa ien C1 M W9 A. eda oanstis M 605 um .ar lce 99 .h A .a W A 40 A M 591 00 31 0 2 9 9 C 0 W J W0 M M MW99393 A.antidolus C42 MW99406 A.antidolus C44 WE02671 mininoides C27 MW00226 A.fe WE0 14 A.femininoides C27 A.m MW 26 286 A.k organi C27 MW999 urdistan icus C55 WE 8205 A MW02591 A .dama MW999292 A .peilei C3 9 .p 9 AD 09 ie MW98024 5 A.hubrceae C21 erti 99 A.h MW 982552 A. uberti C34 MW h 4 MW 9 8 2 0 A u b e r . ti C3 M 9 94 th 3 M W9 934 A.g eres ADW0094791 A.p ueze iae C5 lm i 9 MW e 2 9 A W 9 801832 A .turc rceae avi .e ic M E 9 C MWW0026 508 A.nin lburs ola C 23 6 A M M W 0 00 1 .n ae icus 20 C1 A W 0 00 58 A.a ina 8 M D 0 0 51 A ra e M W 98 031110 A. .elb sba C34 W 98 00 6 A elb urs ran 98 0 1 A .el ur icu i 26 09 A.s .elb bur sic s C 1 A. ur ur sic us 18 A. ca ak sic us C1 sc rm ov us C 7 hu o i C1 18 r ia n 7 ni C 80 A molecular phylogeny of Agrodiaetus Fig. 64. Maximum Parsimony Majority Rule Consensus Tree (ITS-2) 65 s rastu oph icus .the rena don 25 T A.py eumeanus . i 8 020 is MW 9901 134 A.morggest tis V A.a es s MW 9 9 MW 0517 033 A.agerxe us 0 2 8 x ic s MW MW0 104 .arta aur ensi ra 0 A .is l e s MW 5 A r u m r o o s 5 a t 7 r 0 . e 00 09 A .c nt ilu n JC W99 001 61 A A.a urypylaodo s M 99 10 70 .e .p lce gu r K P MW W0 982 03 63 V.a ia M W 93 1 4 em M 9 99 02 .s 0 W M MWW0 34 C M 20 0 W M W 99 47 1 A M .da M W0 ntc W M 9 02 h.X W 82 31 m 9 M 8 12 A e JC W9 315 A. .alc nalc MW M 00 81 A alc es a W 99 99 040 72 .alc es tis s C 59 60 A A es tis C 50 1 5 . .m t i C 1 MWA.hu A.h aroa enas C 21 9 01 me um nie lca20 M MW W0 107 da eda ns s 10 A sa s is 0 MW1039 53 A .fulge C3ae MW JM00 010 A.fab .ainsens 8 004 001 01 A res ae 98 .a s A WE MW0 A.vali .fabreinsaeei 0 024 31 A 064 A abadi ssei . M .k v W a ho li C23 JC00 0 045 1014 A rasaneabadi A.ne .r nsis phoh ipartii C iptam 90 JC0 JC010 0043 A.ripenos 14 A.a artii WE02 d MW9938 677 A.demametus 2A ve i MW00185 .demavendi Cnd A.demavend 70 i C7 MW00015 A.dem avendi C700 MW99407 A.demavendi C60 MW99196 A.ripartii C90 WE02535 A.lorestanus C66 i MW99105 A.demavend ndi ave MW00189 A.dem M 4 ii 2 ss ii C 18 du uss ti C 16 r i C 5 f A. ird es ri 1 9 1 A.f ern ffe ri C C1 15 7 A. hop ffe on 25 00 924 97 A. .hop seid i C C20 m W 9 0 9 M W 98 818 08 AA.po utna idon C21 M W 9 94 0 .p se on M W 9 18 1 A .po eid s s M W 98 50 A po s M W 99 38 A. ciu onide 14 M W 981 54 .ly eid ia C M W 981 79 A pos gen C12 M W 80 A. iphi enia 5 M W9 001 9 A. hig C2 M S00 804 A.ip cicus 24 D W9 009 A.tur icus C M W99 135 .turc rmon M W99 03 A phica C27 M W992 03 A.i ytopi M 981 2 A.ba eri MW 9937 A.tank MW 99565 A.tankeri MW 99559 ytopi MW 99309 A.bavshani MW 0176 A.ro ni 0 MW A.rovsha es WE02662 iphigenid DS01001 A. enes em WE02491 A.acha WE85001 A.shahrami MW00393 A.erschoffii C14 AD98036 A.phyllis MW99174 A.phyllis MW00348 A.phyll is C85 MW00452 A. phyllis C75 MW00 WE02 262 A.klaussc WE00 451 A.tenhag huriani C56 eni MW 002 A.g MW 00101 A laucias MW000347 A .darius .p 0 osth M W 40 MW 005479 A.caeru umus C 85 00 A.b le MW 000444 A. irunii Cus C20 MW b 7 MW 001 2 A. irunii 10 biru C1 A 00 02 M D98 267 A.bir nii C 1 M W9 024 A.b unii 11 MWW999095 A.hu irunii C11 M C 5 A b M W 992 52 .h erti 10 A W 99 26 A.h ube M D 99 37 A ub rti M W9980 479 4 A. .zap erti C34 C 1 z v M W M W 98 950 8 A A.tu apva adi 33 W 98 24 8 .ni rci d 00 29 0 A. na co i C n 1 l e aC 9 11 4 A. in 0 A. the ae 20 A. gu re C el ez sia 34 bu e e rs lma C5 icu v 9 s i C1 8 17 C 17 us C 8 ic s 1 rs icu s C 18 bu rs u C el u sic us A. elb ur ic ni 6 A. elb urs ara 17 22 31 51 A. elb sb C a C 00 0 8 A. ra lae str W 00 05 2 .a au hu M W 00 23 1 A .p rat vi M W 00 66 27 A .za rako on C80 s C22 M W 02 1 1 A .su rm ni nsi M E 00 53 A .ca uria ne 1 W W 02 01 A ch da C2 a M E 0 9 .s W D98980061 A .hamrceae C23 A W 82 2 A .pie eae M W9 003 2 A ierc a M 0 29 A.p MW 99 41 .dam i C39 7 MWW993205 A .peile ani C2 s C27 M W98 91 A .morg oide 27 M E025 14 A minin oides C 55 W 026 A.fe in C WE 00226 A.feministanicus MW 02671 A.kurd s C42 WE 9286 ntidolu 4 MW9 93 A.a tidolus C4 3 9 MW999406 A.an inensis C17 MW 9 A.diz mon C14 MW0053 9 A.iphida MW0026 8 A.iphidamon MW0032 O M K9 W 60 0 2 05 M 2 L 25 M W9 .ca C W M 0 96 el .se M W 11 12 es m MW W0 010 16 L.ctiss iarg im 1 1 L MW MW 010 034 8 L .co orid usus MW 995 010 59 L.c.cor rido on 99 36 92 L L.al ori ido n MW514 L.co .alb bicadonn MW 982 L.co rydo icanns MW 981828 L rydonnius s MW 981 5 L .syr ius i MW 0101129 L. .ossmaca o MW 98278 L.bell ssmaar 9 MW 9608 LL.bellaargusr 004 .be rgu MW069 L.be llarguss llarg MW9 0116 P.a u 8 MW99 220 P rguss 01 .lo MW99 3 N.coelesewii 038 P.c tina MW9823 ornelia MW982645 P.cornelia P.cornelia MW99550 P.m yrr us MW99537 P.myrrhhin inus MW00326 P.aedon JC00039 P.escheri JC00063 P.icarus icus JC00061 P.andron carus MW01025 P.i P.icarus MW00412es forsteri 0 P.eroid roidess o MW0053 JC00042 P.e enela 1 A.m enelaoss 5 0 0 0 JC P.m .icaru 9 2 0 JC00 02006 P.dorylass MW 014 P .dorylaes it P 99 MW 01019 .therssites P s MW 1083 P.theranduus 0 2 m MW 0030 P.a mandtima 7 MW 9904 1 P.a N.fa rona a d 0 MW 020 9301 N.coarci nis MWMW9 0504 M.m aphhnisn d ap o n . 0 0 9 M MW 002 29 M.d .dammo 42 0 a 1 MWW98 076 13 A A.d oi CC3 42 M W00 996 46 enk tus i C 42 5 o h c M W 99 tc je k oi C M W an er en k M .d .int tch en h A 4 A an tc 27 164 A.d dan 9 9 99 6 A. W 7 M MW 92 19 9 3 W 9 M W9 M MW98136 A.wagneri C18 MW98139 A.wagneri C19 MW98313 A.se MW98170 A.m rtavulensis C20 araschi C16 MW99 AD98 240 A.altivagans MW99012 A.altivagans C21 0 5 7 A.m MW MW 99353 A erhaba C17 WE 99465 .altivaga WE002454 4A.kanduli ns C22 MW 2621 21 A.m C25 99 A.s ofi MW 99 094 A ennan dii MW 00 448 A .cyan ensis MW WE 003179 A .cyan eus C1 M 02 30 .cy eus C 7 18 M W0 674 A.p ane M W0 017 A. seu us MWW0 012 8 A. gorbu doxer MW 9 017 9 A gor nov xes 8 7 . g bu M i C1 5 M W 98 20 A. orb no A W 99 28 3 A go un vi M D 0 00 5 .m rbu ov M W 980023 6 AA.sig ithri nov i C20 M W 99 09 4 A .fi be da i C W 98 05 A .f rdu rt tes 20 99 1 8 .p ird ss i C C 41 62 A. se us ii C 25 23 3 A. art uda sii C 30 A. ac vin c 2 fir tis en tis 5 du C s ss 17 is C ii 25 C 25 Chapter 3 Fig. 65. MP Majority Rule Consensus Tree of the shortest trees (ITS-2 & COI) 66 A molecular phylogeny of Agrodiaetus 100 100 70 100 100 100 100 99 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 99 100 100 57 100 58 83 100 78 100 100 100 51 100 100 0 100 100 100 55 55 100 100 69 100 Agrodiaetus MW00076 M.daphnis MW98029 M.daphnis MW00290 M.marcida MW00504 N.corona MW99301 N.fatima MW02001 P.amandus MW99047 P.amandus MW00302 P.thersites MW01083 P.thersites MW01019 P.dorylas MW99014 P.dorylas MW02006 P.icarus JC00029 P.menelaos JC00051 A.menelaos JC00042 P.eroides MW00530 P.eroides forsteri MW00412 P.icarus MW01025 P.icarus JC00061 P.andronicus JC00063 P.icarus JC00039 P.escheri MW00326 P.aedon MW99537 P.myrrhinus MW99550 P.myrrhinus MW98264 P.cornelia MW98235 P.cornelia MW99038 P.cornelia MW99013 N.coelestina MW98270 A.anteros MW01061 A.cramera MW99001 A.torulensis MW99097 A.isauricus JC00055 A.artaxerxes MW01048 A.agestis MW02033 A.agestis MW00024 V.alcedo MW00525 C.semiargus MW02034 C.semiargus MW98220 P.loewii MW00116 P.argus MW00469 L.bellargus MW99608 L.bellargus MW98278 L.bellargus MW01011 L.bellargus MW98129 L.ossmar MW98185 L.ossmar MW98228 L.syriaca MW99514 L.corydonius MW99536 L.corydonius MW01092 L.albicans MW01059 L.albicans MW01034 L.coridon MW01018 L.coridon MW01116 L.coridon MW99612 L.coridon OK96022 L.caelestissimus MW00517 V.morgianus MW99134 A.eumedon MW99163 P.pylaon MW99303 K.eurypilus MW99018 A.pyrenaicus MW02025 T.theophrastus Aricia Lysandra Fig. 66. MP Consensus Tree of the combined data set (ITS-2 & COI) excl. Agrodiaetus 67 Chapter 3 100 100 100 76 100 100 100 100 99 100 75 100 99 100 99 100 100 90 53 83 55 53 100 75 100 100 53 66 100 100 100 59 100 74 100 100 100 100 65 99 61 100 100 100 58 63 100 100 76 100 51 100 100 100 100 100 100 100 100 72 100 100 100 100 100 100 100 100 92 100 100 100 100 100 100 100 90 100 99 100 100 88 100 100 100 56 66 100 58 91 100 100 92 100 100 69 100 100 100 100 71 100 100 100 100 100 100 100 100 100 100 100 MW98136 A.w agneri C18 MW98139 A.w agneri C19 MW98313 A.sertavulensis C20 MW98170 A.maraschi C16 MW99240 A.altivagans C21 AD98012 A.altivagans MW99057 A.merhaba C17 MW99353 A.altivagans C22 MW99465 A.kanduli C25 WE02454 421 A.mofidii WE02621 A.sennanensis MW99094 A.cyaneus C17 MW99448 A.cyaneus C18 MW00179 A.cyaneus MW00330 A.pseudoxerxes C15 WE02674 A.gorbunovi MW00178 A.gorbunovi MW00129 A.gorbunovi C20 MW00177 A.gorbunovi C20 MW98203 A.mithridates C23 MW98285 A.sigberti C25 MW99006 A.firdussii C30 MW00234 A.firdussii C25 MW99058 A.artvinensis C25 AD98009 A.pseudactis MW98162 A.actis C17 MW99413 A.firdussii C25 MW00151 A.firdussii MW99247 A.firdussii C24 MW98097 A.ernesti C18 MW98079 A.lycius MW98189 A.hopfferi C16 MW99408 A.hopfferi C15 MW98180 A.poseidon C19 MW99501 A.putnami C25 MW98138 A.poseidon C20 MW98154 A.poseidon C21 DS00001 A.poseidonides WE02491 A.achaemenes WE85001 A.shahrami MW00393 A.erschoffii C14 AD98036 A.phyllis MW99174 A.phyllis MW00348 A.phyllis C85 MW00452 A.phyllis C75 MW00262 A.klausschuriani C56 WE02451 A.tenhageni WE00002 A.glaucias MW00101 A.darius MW00347 A.posthumus C85 MW00409 A.caeruleus C20 MW00444 A.birunii C11 MW00547 A.birunii C10 MW00072 A.birunii C11 MW00102 A.birunii C11 MW00267 A.birunii C10 DS01001 A.iphigenides MW98049 A.iphigenia C14 MW99009 A.iphigenia C12 MW99135 A.turcicus C25 MW99203 A.turcicus C24 MW98103 A.iphicarmon MW99372 A.baytopi C27 MW99565 A.tankeri MW99559 A.tankeri MW99309 A.baytopi MW00176 A.rovshani WE02662 A.rovshani MW99292 A.pierceae C21 MW99341 A.pierceae C23 MW98240 A.theresiae C59 MW98294 A.guezelmavi MW99393 A.antidolus C42 MW99406 A.antidolus C44 MW99286 A.kurdistanicus C55 MW00226 A.femininoides C27 WE02671 A.femininoides C27 WE02614 A.morgani C27 WE02591 A.peilei C39 MW98205 A.dama MW00110 A.elbursicus C18 MW00316 A.elbursicus C17 MW00051 A.elbursicus C17 MW00058 A.elbursicus C18 MW00232 A.elbursicus C18 WE02661 A.arasbarani MW00127 A.paulae C17 WE02531 A.zarathustra C22 MW00032 A.hamadanensis C22 AD98001 A.surakovi MW98009 A.carmon MW98261 A.schuriani C80 AD98018 A.ninae MW99508 A.ninae C34 MW99479 A.turcicola C20 MW99226 A.zapvadi MW99374 A.zapvadi C19 AD98024 A.huberti MW99095 A.huberti C34 MW99552 A.huberti C33 MW00539 A.dizinensis C17 MW00269 A.iphidamon C14 MW00328 A.iphidamon MW99382 A.demavendi C70 WE02677 A.demavendi MW00185 A.demavendi C70 MW00015 A.demavendi C70 JC01014 A.admetus JC00043 A.ripartii JC00045 A.nephohiptamenos MW99407 A.demavendi C60 MW99196 A.ripartii C90 WE02535 A.lorestanus MW00189 A.demavendi MW99105 A.demavendi C66 MW01014 A.ripartii C90 WE02431 A.khorasanensis MW00064 A.valiabadi MW00498 A.valiabadi C23 JM00001 A.fabressei MW01001 A.ainsae MW01039 A.fabressei MW01053 A.ainsae MW01107 A.fulgens MW99591 A.humedasae C38 MW99605 A.humedasae JC00040 A.aroaniensis MW98172 A.menalcas MW98315 A.alcestis C20 MW98212 A.alcestis C21 MW00231 A.alcestis C19 MW99471 A.dantch.Xmenalcas C50 MW99319 A.dantchenkoi C42 MW99276 A.dantchenkoi C42 MW99164 A.interjectus C31 MW99274 A.dantchenkoi C42 MW99546 A.damon MW99613 A.damon Outgroup poseidon-group erschoffii-group iphigenia-group carmon-group iphidamon-group admetus-group dolus-group Fig. 67. MP Consensus Tree of the combined data set (ITS-2 & COI) of Agrodiaetus 68 A molecular phylogeny of Agrodiaetus 55 Agrodiaetus & Polyommatus (partim) 100 61 57 77 87 61 96 100 99 64 100 100 96 56 55 93 98 99 94 64 99 62 54 65 100 59 98 50 91 55 100 100 64 50 96 59 100 56 97 0 100 Fig. 68. Bootstrap Tree species 99 81 AF170864 Euphilotes bernardino MW01061 A.cramera MW99001 A.torulensis MW99097 A.isauricus MW01048 A.montensis MW02033 A.montensis JC00055 A.artaxerxes MW99028 A.agestis MW00020 A.agestis JC00057 A.artaxerxes JC00062 A.agestis MW00024 V.alcedo MW99430 V.alcedo MW00116 P.argus MW99124 P.christophi MW00326 P.aedon MW99538 P.myrrhinus MW99537 P.myrrhinus MW99550 P.myrrhinus MW98264 P.cornelia MW98235 P.cornelia MW99038 P.cornelia MW98228 L.syriaca MW99140 L.corydonius MW99514 L.corydonius MW98185 L.ossmar MW99042 L.corydonius MW99536 L.corydonius MW98129 L.ossmar MW00469 L.bellargus MW98278 L.bellargus MW99435 L.bellargus MW99608 L.bellargus MW01011 L.bellargus OK99001 L.gennargenti MW01116 L.coridon OK96022 L.caelestissimus MW01103 L.coridon MW99612 L.coridon MW01092 L.albicans MW01059 L.albicans MW01018 L.coridon MW01034 L.albicans MW00517 V.morgianus MW00525 C.semiargus MW02034 C.semiargus MW98220 P.loewii MW98270 A.anteros MW99013 N.coelestina WE00003 N.diana MW99018 A.pyrenaicus MW99134 A.eumedon MW99163 P.pylaon MW99303 K.eurypilus AF170863 Apodemia mormo MW99520 L.thetis MW98230 L.tityrus MW98094 L.thersamon MW02009 L.phlaeas MW00119 L.candens MW99515 L.virgaureae MW00044 L.asabinus MW00017 L.alciphron MW02007 L.alciphron MW00497 F.quercus MW01022 L.boeticus MW02028 L.boeticus MW98265 L.boeticus MW01023 S.pirithous MW99158 S.hyrcanicum MW01027 S.esculi MW99398 S.myrtale MW01120 C.marshalli MW02008 C.argiolus MW99084 C.argiolus MW02021 Z.knysna MW02025 T.theophrastus MW02031 P.abencerragus MW99080 P.vicrama MW98002 C.osiris MW99045 M.arion MW99221 T.endymion MW99425 C.trochylus MW02024 I.feisthamelii JC02001 I.podalirius AF170873 Iphiclides podalirius MW01114 I.feisthamelii AF044007 Colias eurytheme AF170860 Coenonympha tullia AF044006 Papilio machaon (COI) excl. Agrodiaetus and closely related Polyommatus 69 Chapter 3 9 on C7vi A.carm rako 90608001 A.sgului C4o6n MW9 m 9 io D A kerc .car 25 A.se 09 A mi C 155 9289MW98A0.putnpafferii C C2 MW9 ho m 25 01 995 8 A. tna is C 30 MW 99401 A.puinensssii CC256 MW9906 .artv.firdubertiri C119 MW9058 A06 AA.sigopffeon CC19rti 9 9 0 5 . h id n e 7 MWMW9 982889 Aposeeido.sigbC121 s s . 0 MWW98183 A .po 84 A.actoi n CC2 18 s M 981 80 A982 2 A eid on ti Cciuus 1 6 s id s ly i 5 MW 98 MW 81 po se e . .lycC2 4 A n . r 9 MW MW 4 AA.poA.e089 9 A sii i C2 15 8 7 8 7 s si 98 813 809 W9 980firdu us d MWW9 W9 M W A. .fir M 34 A M M 2 7 0 0 24 W 99 M W M rti C33 A.hube C34 99555028 A.ninaeol MW 9 9 A.turcic a C20 MW999 47 ninae MW9801 A. AD 0284 A.huberti AD98 53 A.huberti C36 MW990 A.huberti C34 MW99095 MW99476 A.zapvadi C19 MW99374 A.zapvadi C19 MW99226 A.zapvadi MW99314 A.tu MW98049 A. rcicola C20 iphigenia C1 AD 4 9 A.iphige MW9802 nia 10 A.ip MW998 higenia 90096 A MW C15 .ip 9 WE029170 A.i higenia C phig 12 49 W W E850 1 A.ac enia C WEE00245011 A.shahaemene 12 MW A.ten hrami s 0 003002 A.g MW h MW 003 47 A laucageni ias MW 0 58 .p MW 0004335 AA.poossthum MW u MW 00003209 A. .caertuhumu s C85 7 c s M M W 00 393 A. aer leus ADW0002259 A.eerschuleus C20 M 6 A r o MWW098001012 A. .klauscho ffii C20 M 9 0 36 A kla ss ffii W 9 1 4 A . d u s ch C 1 MM M WW090911740 A..phyarius schuurian4 ria i C MMWW0004 3487 AA.pphy llis n i 57 W 0 04 5 8 A .p hy llis C5 00 02 44 2 A .p hy llis 6 54 67 A .p hy llis 7 A. .bi hyl llis A. bi ru lis C bi ru nii C 85 ru ni C 75 ni i C 1 1 iC 1 10 0 W 99 40 7 M M A. M W9 MWW9dem MWW9 91 9 92 a 91 02 9 6 ve W M E 9 9 9 A 26 4 n MW M W0 025 068 6 A .de3 AA.rdi C W 3 0 99 9 18 5 A. .rip ma .ripipa 60 r MW105 91419 AA.lo iparartii venartrtii 99 A.de A. .demres tii CC9 di ii 1 d m MW M 04 a em avtanu 900 010W01 A.d ven aveends WE 14 072emadi C nd i 02 4 MW A i 31 .r pvend66 A.k0110.ripaA a i hor 5 A rtii iC asa .rip 9r0tii nen arti s is i 11 1 iC 1 ni i C 11 ru ni C 11 s bi ru nii C de A. bi ru nii ni 6 A. .bi iru ido ra s 47 02 A .b se a ide es teri 00 01 60 2 A .po gm en id rs W 0 00 7 A .da hig en fo ae M W 0 000 015 A .ip phig idesildiz i 0 W A M MMW 0000001 1 A. .eroros ylaos DSS0010800 30 PP.e ene laos D S 9 05 79 .m ne s D E e W W099529 PP.m roideus M W 00 51 P.e icar s M C0 00 42 P. aru J 0 s 3 ic JCC009081325 P. .icaruonicus J W 010 2 P ndr M W 041 P.a arus M W0 061 P.ic heri M 0 3 sc eri h c JCC0000639 P.e.e J 000 009 P asbressei 1 JCW .f ae i M 00000101AA.ainsre sse 0 JMW 1 .fab ens M W001039 A lg M 01107 A.f.auinsae MW 01053 A nsae MW A.ai 8 07 MW01 valiabadi MW00064 A. A.valiabadi C23 MW00498A.aro aniensis JC00040 MW99591 A.humedasae C38 MW99605 A.humedasae MW98020 A.men MW98172 A.m alcas ena lcas MW MW 99494 A.menalcas MW 98315 A.alcestis C2 0 M 98212 A.alcestis M W99164 C21 M W99471 A.interject M W9938 A.dantc us C31 M W993 0 A.alc h.Xme M W99 20 A.d estis C19 nalcas C50 M W9 319 A antc W 9 9276 .dan henko MW 0 9274 A.da tchen i C41 MW 0002229 AA.dannttchenkkoi C42 oi C ch 31 .a A .alclcestisenkoi C 42 estis C19 42 C19 M M M W M W 99 W 9 4 0 9 AD M 42 13 A015 AD 98 W002 A .fir 1 A . 0 W 98 09 12 fird du .fir W MW M E02 WE0 012 A. 5 A ussssii du 25 A p . f i C s W 3 MW9831 990 21 AE02 36 .alseu irdui C2 25sii 4 A ti d s 5 3 5 M 982 A.s 7 A .ps 21 .davagac si MWW98 03 A erta.meeud A.m maantis i MW 991 170 .mithvulerhaboxerofidlis s r n a x i 9 6 A MW92405 A.a.mar idatesis CC1es i MW 9813 A.altltivagasch s C2207 M W 994 WE0 981369 A.wivagaansi CC163 65 A 262 A. ag ns 2 .dam 1 A wag neri C215 oc .sen ner C1 MW9 MW99le4s kanndaneni C189 9448 49 A uli C sis .cya 25 MW99MW9935A7.cyane us neus 3 MW9 53 A.altivA.altivagC18 0 9 4 A.c agans Ca2n2s MW99905 9 A yaneus C MW .m 00 er 33 haba C117 0 A.pseud MW0017oxerxes C157 A.cyaneu MW00178 9A.g orbunovis WE02675 A.gorbun ovi MW00177 A.gorbunovi C19 C20 MW00129 A.gorbunovi C20 up gro n Ou.tdamoon m 3 A 13 .da C2 1 996 6 A ae C2 s MW 995p4iercceeaercicu25 MW1 A. .pier A.tuus CC24s 4 A 6 c u 993 92 952 urci icus nd 80 i MW 992 W95 A.t.turc.amani Clma5v9 MW M913 3 A 7 P uriaezee C mas 9 20 04 ch u ia da ru s MWW99 99 A.s A.g res5 A. .icandulas s M MW 61 94 .the 20 6 Pma oryryla 2 2 98 98 0 A 98200P.a P.d.do MW MW824 MWW0 01 14 8 P 9 M 0 0 2 0 2 99 1 MW W W 98 M M W M M MW M W 01 M MW W9 99 01 M W 0 9 4 9 M W 98 05 30 69 P. MW M MWW9 003 008 04 1 NN.fdor MW 00 W0 01 91202 P. N.c .fa atimyla MW 004269 032 0838 PP.thther orotim a s a . s 8 MW MW 005324 AA.iph A.i P.ththeer rsi itesna 99 99 9 A .iph ida phi er sit tes MW286 A376 A .diz idammondamsiteess in MW MW99939.3kurdi.antidenson CC1o4n i MW 99473 9406 A.anstanicoluss C114 002 A.k A.a tido us C3 7 WE026 A.feurdistnatidolulus CC550 WE0 2612 Amininonicuss C4442 id C 26 .k W 14 A arin es C 65 WE0 2671 E02591.morgdaus C627 n A A i .f .p C e m eil 26 MW99 37 ininoidesei C397 C2 MW929A.baytop 344 A.b i C277 MW9810 3 .iphi aytopi MW99A rm 565 A.ca tankon MW9955 A.tankeerrii MW993099 A.b ayt WE02662 A.rovshaopi MW00176 A.rovshani ni MW00087 M.marcida MW00076 M.daphnis aphnis MW98029 M.d daphnis MW982740 M. M.marcida MW0029A.elbursicus 056 rsicus C178 MW00A u 1 51 .elb icuss C C 16 MW000058 A.elbuurs rsicuus C18i ic baran2 MW000059 A.elb rs u lb MW0 0110 A.e1 A.arasstra C2lae 6 rathu .pau 17 MW0WE026.z A a 157 A lae C 18 2531MW00 A.pasuicus CC172 r us 2 WE0 27 001 elbu rsic s C sis MW232 A. .elbunensai nenC21 0 A a 0 0 d ad is C7 0 6 MW 0031hama.hamnensendi i C770 i MW32 A. 01 A adamavvenddi Cenddi n 0 000 00 .ham .de ma ve avven 70 i MW MW76 A 83 AA.deema.demmadi Cendus 26 001015 A.d 6 A .deven av et tus tii 0 A WE MW 00 185 01881 ma.dem.admmeiparos s MWW00 W0 993 .de7 A 4 A .adA.r en no M W 2A 7 8 A 3 m e M M 38 026 80 14 04 ipta am 99WE W9010 00 oh hipt M JC JC ph o MW h ne p A. .ne 46 5 A 0 00 004 JC C0 J Fig. 69. Bootstrap Tree (COI) of Agrodiaetus and closely related Polyommatus species (50%) 70 us iarg us .sem iarg us 34 CC.semhrast ilus p p n 020 MW 00525T.theo .eurypylaoon MW 2025 303 K63 P. medicuss 0 99 1 eu a u MW MW W99 4 A. yren ian ar M 13 .p org sm ar 99 18 A .m .os sm aca s MW990 17 V85 L L.ossyri niuius . o n 5 1 MW 00 98 129 8 L ryd don ido on MW MW 98 822L.co ory .cor rid MW W9 14 L.c 4 L .co M 5 6 3 L 99 53 0 18 W 9 01 0 M W9 W 01 M M W M 34 ti C er erti b 75 hu ub llis C A. A.h phy yllis 85 s 4 . s h i 02 95 4 A .p yll lis C ide 98990 17 2 A .ph hyl idon des 8 AD W 99 04536 A A.p ose eni sis e C3 M W 0 0 48 .p hig ien sa M W 98 03 1 A .ip an da e M D 0 00 A aro me asa A W 00 01 A. hu ed M S 10 40 A. um nos D S0 00 91 A.h rtii ptame D C0 995 05 ripa ohi J W 96 A. eph us M W9 043 A.n met nensis M C00 045 A.ad rasa J C0 0 1 4 ho 0 ei J 010 31 A.kabress endi C7 C22 JCE02401 A.f emav nensis W 000 15 A.d mada JM 000 2 A.ha badi MW 0003 A.valia di C23 MW 00064 .valiaba MW 00498 A irunii C11 MW 00072 A.b ius MW 101 A.dar 5 MW00 347 A.posthumus C8 MW00 irunii C11 MW00102 A.b unii C10 MW00267 A.bir WE02531 A.zarathustra C22 MW00127 A.paulae C17 MW99226 A.zapvadi MW99374 A.zapv adi C19 MW00330 A. pseudo WE0 74 A.gorbun xerxes C15 MW 26 ovi 178 A.g MW000 MW 0129 A.goorbunovi rbunov MW000177 A.g 0 o WE0 176 A rbuno i C20 vi 26 .r MW 001 62 A.roovshani C20 MW 7 v s 9 MW 0018 A.c hani y an W 00 5 M E02 189 A.de eus M W9 591 A.d mave MWW998205 A.peemave ndi C7 ile 2 0 nd A W W E 002 86 .da i C39 i M E 026 26 A.k ma M W 02 14 A. urd M W9993671 A.mfeministani c M W 9 A i M W 0 94 3 .fe org no us M W 0 02 06 A. m an ide C5 M W 00 026 31 A.a anti inino i C2 s C2 5 d W 00 2 2 A . nt o id 7 7 00 3 69 A. alc ido lus es 39 28 A. kla es lus C4 C2 3 A. iph us tis C 2 7 4 A. iph id sc C1 4 er id am hu 9 sc a o ria h o mo n C n i ffi n 1 C5 4 iC 6 14 W W E0 E 0 W 249 253 E 1 W 02 A 5 A E 4 .a . M 000 51 chalore MW MW W 0 A s MW 99 99 MW 995 2 A .ten emetan 99 407 408 995 65 .gla ha ne us g 3 5 A A MW 82 .d A.h 9 A .ta uc en s MW MW9 993 A.deema opff .ta nkeias i 993 934 72 ma ven eri nke ri 19 1 A A.b ven di C1 ri A.d .pi ayt di C6 5 e o C 0 a M MW W99 MW9 ntcherceaepi C 70 292 930 nk C 27 9 9 2 MW 992 76 A.d A.pier9 A.boai C423 74 an ce y 2 M A.da tche ae top MW9 W9919 ntchennkoi CC21 i 6 A.r koi 42 9164 A.inte iparti C42 MW9 9 rjectu i C90 MW 203 A.turc s C3 icus C 1 MW99199135 A.turc 24 05 A.de maveicnus C25 MW9944 d 8 A.cyane i C66 MW99094 C18 A.cyaneuus s C1 MW99009 A.iph igenia C127 MW99006 A.firdussi i C30 MW99471 A.dantch.Xmenalcas C50 MW98315 A.alcestis C20 20 C s 7 eu 1 1 ul C1 C er ii sis 0 ca run en C1 A. bi in ii 9 A. diz un e 90 40 44 A. .bir nsa tii C i 00 4 39 A .ai ar sse W 00 5 47 A rip re M W 00 05 001 A. .fab sae s M W 0 1 14 A ain en M W 0 10 39 A. lg on C80 i M W 4 fu M W00100537 A. .carmurian a C1 M W 01 10 A sch eni M W 01 009 A. hig M 8 s 8 1 ip MWW9982649 A. .lyciu sti C1 n M W 80 9 A erne rmo M W9 807 A. hica i C25 M 9 97 .ip m 0 MW 980103 AA.putnaidon C2 1 MW 98 501 .pose on C2 MW 99 138 A oseid s MW A.p lca 98 MW 8154 .mena n C19 MW998172 AA.poseido C16 MW 98180 .hopfferi MW 98189 A ithridates C23 MW 03 A.m 21 MW982212 A.alcestis C C59 MW98 theresiae MW98240 A. elmavi MW98294 A.guez MW98285 A.sigberti C25 M OK M W 9 6 M W 01 0 2 W 01 0 5 2 0 09 9 M MW L.c 111 2 L L.a MWW9 99 aele 6 L .alblbic M 0 827 612 sti .co ic an MWW99 1011 8 L L. ssim rid ans s 0 60 L .be cor u on MW04698 L. .bel llar ido s MW 001 L.bbellalarggus n M 9 16 ell rg us M W00 8220 P. arguus MWW020 024 P.loargu s s 3 V M 0106 3 A .alc ewi JC0 W010 1 A.c.agesedo i MW 0055 A48 A.aramertis e a MW9 99097 A.artaxg erxstis 90 .i MW9 01 A.tosr auricuess 8 u 2 le MW99 7 013 0 A.an nsis MW9 N.coele teros na MW990 9301 N.fastitim 47 P.am MW02001 andusa P.amandu s MW00504 N. corona MW99038 P.co rnelia MW98235 P.cornelia MW98264 P.cornelia MW99550 P.myrrhinus yrrhinus MW99537 P.mP. aedon MW00326 dorylas P. 4 01 s 99 W M .doryla P 9 01 MW01 3 P.thersiteess it 08 rs 1 e 0 h W .t s M 302 P .icaru MW00 02006 P aphnisis MW 029 M.d aphn a .d id 8 c 9 M MW 00076 M.marcheri MW 00290 9 P.esicaruss MW 0003 63 P. onicurus JC 000 ndr .ica os JC 1 P.a 5 P nela es 6 02 e id s 000 01 .m ro ao ri JC MW 51 A 2 P.eenel rste us 00 004 .m s fo icar on 0 JC JC0 29 P ide P. am on ii 0 o 2 .d m id i 00 .er 41 A .da of m i JC 30 P 00 613 6 A A.m hraendis 5 MW 99 954 54 sha av ns 00 MWW9 024 A. em ane MW M E 001 A.d nn W 5 7 se E8 67 A. W 02 21 E W 026 E W MW98136 A.wagneri C18 MW98139 A.wagneri C19 AD98012 ltivagans MW98170A.a MW98313 A.maraschi C16 A.sertavu MW9905 lensis C20 7 A.mer MW MW 99240 A.a haba C17 MW999353 A.a ltivagans C 21 ltivag AD 9465 MW98001 AA.kandu ans C22 99 li C .s MW 992413 A.fiurakovi 25 MW M 99 47 A rdus M W9 058 .fird sii C2 ADW008162 AA.artv ussii C 5 24 9 23 MW .a ine M 0 800 4 A ctis nsis C 25 M W 01 9 A .fird C1 ADW0994751 A .pseu ussii 7 C 0 9 .f M d W W99801232 A.tuirdus actis 25 s A M E M W 0 95 8 A .e rcic ii M W 0 26 08 .n lbu ola C M W 0 0 61 A ina rs M W 00 00 058 A.a.nin e icus 20 W 00 1 51 A ra ae C1 99 3 10 A .el sb C3 8 55 16 A. .elb bur ara 4 2 A. elb ur sic ni A. el u sic us hu bu rsi us C b e r s i c us C 1 8 rti cus C 17 C C 18 33 1 7 A molecular phylogeny of Agrodiaetus Fig. 70. Bootstrap tree (ITS-2), condensed at 50% confidence level 71 Chapter 3 95 60 90 100 87 59 73 100 81 84 56 95 89 100 99 73 95 100 100 100 100 100 84 100 67 84 97 0 82 80 86 55 98 96 90 100 51 99 53 52 100 100 Agrodiaetus MW00530 P.eroides forsteri JC00029 P.menelaos JC00051 P.menelaos JC00042 P.eroides MW00412 P.icarus MW01025 P.icarus JC00061 P.andronicus JC00063 P.icarus JC00039 P.escheri MW00290 M.marcida MW00076 M.daphnis MW98029 M.daphnis MW00302 P.thersites MW01083 P.thersites MW01019 P.dorylas MW99014 P.dorylas MW00504 N.corona MW99301 N.fatima MW02001 P.amandus MW99047 P.amandus MW02006 P.icarus MW00326 P.aedon MW99537 P.myrrhinus MW99550 P.myrrhinus MW98264 P.cornelia MW98235 P.cornelia MW99038 P.cornelia MW99013 N.coelestina MW98270 A.anteros MW01061 A.cramera MW99001 A.torulensis MW99097 A.isauricus JC00055 A.artaxerxes MW01048 A.agestis MW02033 A.agestis MW00024 V.alcedo MW00116 P.argus MW00469 L.bellargus MW99608 L.bellargus MW98278 L.bellargus MW01011 L.bellargus MW98129 L.ossmar MW98185 L.ossmar MW98228 L.syriaca MW99514 L.corydonius MW99536 L.corydonius MW99612 L.coridon OK96022 L.caelestissimus MW01116 L.coridon MW01092 L.albicans MW01059 L.albicans MW01018 L.coridon MW01034 L.albicans MW00517 V.morgianus MW00525 C.semiargus MW02034 C.semiargus MW98220 P.loew ii MW99018 A.pyrenaicus MW99134 A.eumedon MW99163 P.pylaon MW99303 K.eurypilus MW02025 T.theophrastus Aricia Lysandra Fig. 71. Bootstrap Tree of the combined data set (ITS-2 & COI) excl. Agrodiaetus 72 A molecular phylogeny of Agrodiaetus 57 51 79 53 98 100 96 99 70 99 53 68 72 70 85 67 83 92 58 76 59 73 95 69 74 84 54 59 89 87 66 66 93 63 100 72 80 57 95 99 57 51 99 97 93 57 64 52 69 74 100 99 97 80 91 100 68 68 83 60 58 63 95 73 54 73 97 100 70 100 70 76 89 78 98 99 100 99 100 99 MW98136 A.wagneri C18 MW98139 A.wagneri C19 MW98170 A.maraschi C16 MW98313 A.sertavulensis C20 MW99240 A.altivagans C21 AD98012 A.altivagans MW99057 A.merhaba C17 MW99353 A.altivagans C22 MW99465 A.kanduli C25 MW00330 A.pseudoxerxes C15 WE02674 A.gorbunovi MW00178 A.gorbunovi MW00129 A.gorbunovi C20 MW00177 A.gorbunovi C20 MW00179 A.cyaneus MW98203 A.mithridates C23 MW99094 A.cyaneus C17 MW99448 A.cyaneus C18 WE02621 A.sennanensis WE02454 421 A.mofidii MW99058 A.artvinensis C25 MW98285 A.sigberti C25 MW99006 A.firdussii C30 MW98162 A.actis C17 MW00234 A.firdussii C25 AD98009 A.pseudactis MW99413 A.firdussii C25 MW00151 A.firdussii MW99247 A.firdussii C24 MW98079 A.lycius MW98097 A.ernesti C18 MW98180 A.poseidon C19 MW99501 A.putnami C25 MW98138 A.poseidon C20 MW98154 A.poseidon C21 MW98189 A.hopfferi C16 MW99 408 A.hopfferi C15 MW99292 A.pierceae C21 MW99341 A.pierceae C23 MW98240 A.theresiae C 59 MW98294 A.guezelmavi MW99393 A.antidolus C 42 MW99406 A.antidolus C 44 MW99286 A.kurdistanicus C 55 MW00226 A.femininoides C 27 WE02671 A.femininoides C27 WE02614 A.morgani C27 WE02591 A.peilei C39 MW98205 A.dama MW00110 A.elbursicus C 18 MW00316 A.elbursicus C 17 MW00051 A.elbursicus C 17 MW00058 A.elbursicus C 18 MW00232 A.elbursicus C 18 WE02661 A.arasbarani MW00127 A.paulae C17 WE02531 A.zarathustra C2 2 MW00032 A.hamadanensis C 22 AD98001 A.surakovi MW98009 A.carmon MW98261 A.schuriani C 80 MW99226 A.zapvadi MW99374 A.zapvadi C19 AD98018 A.ninae MW99508 A.ninae C34 MW99479 A.turcicola C20 AD98024 A.huberti MW99095 A.huberti C34 MW99552 A.huberti C33 WE02491 A.achaemenes WE85001 A.shahrami WE02451 A.tenhageni WE00002 A.glaucias MW00393 A.erschoffii C 14 MW00262 A.klausschuri an i C56 AD98036 A.phyllis MW99174 A.phyllis MW00348 A.phyllis C85 MW00452 A.phyllis C75 MW00101 A.darius MW00347 A.posthumus C85 MW00409 A.caeruleus C20 MW00547 A.birunii C10 MW00444 A.birunii C11 MW00267 A.birunii C10 MW00072 A.birunii C11 MW00102 A.birunii C11 DS0 0001 A.poseidonides DS01001 A.iphigenides MW00064 A.valiabadi MW00498 A.valiabadi C2 3 JM00001 A.fabressei MW01001 A.ainsae MW01039 A.fabressei MW01053 A.ainsae MW01107 A.fulgens MW99591 A.humedasae C 38 MW99605 A.humedasae JC00040 A.aroaniensis MW98172 A.menalcas MW98315 A.alcestis C20 MW98212 A.alcestis C21 MW00231 A.alcestis C19 MW99471 A.dantch.Xmenalca s C50 MW99319 A.dantchenkoi C42 MW99 276 A.dantchenkoi C 42 MW99164 A.interjectus C31 MW99274 A.dantchenkoi C42 WE02431 A.khorasanensis MW01014 A.ripartii C90 MW00189 A.demavendi MW99105 A.demavendi C66 WE02535 A.lorestanus MW99196 A.ripartii C90 MW99407 A.demavendi C60 JC00043 A.ripartii JC00045 A.nephohiptamenos JC01014 A.admetus WE02677 A.demavendi MW99382 A.demavendi C70 MW00015 A.demavendi C70 MW00185 A.demavendi C70 MW98103 A.iphicarmon MW99372 A.baytopi C27 MW99565 A.tankeri MW99559 A.tankeri MW99309 A.baytopi MW00176 A.rovshani WE02662 A.rovshani MW00539 A.dizinensis C17 MW00269 A.iphidamon C14 MW00328 A.iphidamon MW98049 A.iphigenia C14 MW99009 A.iphigenia C12 MW99135 A.turcicus C25 MW99203 A.turcicus C24 MW99546 A.damon MW99613 A.damon Outgroup poseidon-group carmon-group erschof fii-group dolus-group admetus-group baytopi-species-group iphidamon-group Fig. 72. Bootstrap Tree of the combined data set (ITS-2 & COI) of Agrodiaetus 73 Chapter 3 Statistical parsimony networks The following table gives an overview of the statistical parsimony networks calculated from the COI data set. The parsimony connection limit was 11 steps for a parsimony probability of 95%. Such a connection limit would only allow the calculation of networks for populations of the same species or very closely related species. In the case of Agrodiaetus 33 different networks were obtained with this connection limit. On the other hand, calculation time increases more than exponentially with an increasing connection limit which rendered the calculation of a complete network for Agrodiaetus unfeasible. Instead, networks were calculated for the main Agrodiaetus clades which were obtained from the combined COI- and ITS2-analysis (i.e. all clades with more than two sequences). The connection limit was increased until all haplotypes were connected, with the only exception of A. klausschuriani in the erschoffii-group, a taxon which was so distant that it seemed more reasonable to exclude it from the analysis. The number of steps required is stated in Tab. 9. Tab. 9. Connection limits in the COI network analysis Group Iphiclides Polyommatus-aedon-group Aricia-agestis-group Lysandra Agrodiaetus-admetus-group Agrodiaetus-menalcas-group Agrodiaetus-carmon-group Agrodiaetus-erschoffii-group Agrodiaetus-iphigenia-group Agrodiaetus-poseidon-group sequences 4 7 12 21 27 25 44 24 16 49 excluded taxa A. klausschuriani haplotypes 3 6 11 19 19 17 34 21 15 39 steps fig. 14 31 13 18 15 16 20 18 18 13 Fig. Fig. Fig. Fig. Fig. Fig. Fig. Fig. Fig. Fig. 73 74 75 76 77 78 79 80 81 82 The number of haplotypes is always lower than the number of sequences, because some sequences turned out to have the same haplotype (or only differed in ambiguities). In these cases, the graph shows only one representative haplotype and the size of the bounding box is increased according to the frequency of this haplotype. Tab. 10 lists those representative haplotypes together with the codes of the collapsed haplotypes. Usually these collapsed haplotypes belong to the same taxon as the reference specimen, but in some cases they were designated to different taxa which is indicated with an asterisk (*). Identical COI haplotypes were found in the following different taxa. • Iphiclides feisthamelii and Iphiclides podalirius • Agrodiaetus zapvadi and Agrodiaetus turcicola • Agrodiaetus kurdistanicus and Agrodiaetus antidolus • Agrodiaetus peilei and Agrodiaetus morgani • Agrodiaetus fabressei and Agrodiaetus ainsae • Agrodiaetus cyaneus and Agrodiaetus merhaba • Agrodiaetus sertavulensis and Agrodiaetus wagneri This indicates that taxa could be identical genetically or that gene flow persists between them. If the bounding box is a rectangle, this haplotype was calculated to have the highest outgroup probability. In several cases the networks provide information which could not be obtained from the phylogenetic trees. Extant ancestral haplotypes appear in the Polyommatus-aedon-group 74 A molecular phylogeny of Agrodiaetus (Polyommatus myrrhinus, Fig. 74), in the Agrodiaetus-menalcas-group (Agrodiaetus dantchenkoi and Agrodiaetus fabressei, Fig. 78), in the Agrodiaetus-carmon-group (Agrodiaetus surakovi, Fig. 79), and in the Agrodiaetus-poseidon-group (Agrodiaetus putnami and A. pseudactis, Fig. 82). Crosslinks between haplotypes of different taxa indicate that gene flow persists between them. This appears to be the case in the species pairs Lysandra corydonius and Lysandra ossmar (Fig. 73), Lysandra coridon and Lysandra albicans (Fig. 76), Agrodiaetus demavendi and Agrodiaetus ripartii (Fig. 77), and Agrodiaetus huberti and Agrodiaetus ninae (Fig. 79). Tab. 10. Collapsed haplotypes in the COI network analysis Group Iphiclides Aricia Lysandra P. aedon A. carmon A. dolus A. iphigenia A. erschoffii A. admetus A. poseidon Reference specimen code MW01114 JC00062 MW00469 MW99140 MW99537 MW00051 MW99226 MW99286 WE02591 MW00032 JM00001 MW00064 MW00229 MW99274 MW99009 MW0060 JC00045 MW00183 MW99068 MW99104 MW99263 MW99382 MW99006 MW00129 MW99413 MW99448 MW98313 MW99061 Taxon specimen codes of collapsed haplotypes * taxon different from the reference taxon I. feisthamelii A. agestis L. bellargus L. corydonius P. myrrhinus A. elbursicus A. zapvadi A. kurdistanicus A. peilei A. hamadanensis A. fabressei A. valiabadi A. alcestis A. dantchenkoi A. iphigenia A. birunii A. nephohiptamenos A. demavendi A. ripartii A. demavendi A. ripartii A. demavendi A. firdussii A. gorbunovi A. firdussii A. cyaneus A. sertavulensis A. putnami AF170873* MW00020 MW99608 MW99514 MW99550 MW00056 MW99314* MW99376* WE02614* MW00001 MW01001* MW01039 MW00231 MW99276 MW99170 MW00060 JC00046 MW00185 MW99196 MW99141 MW99264 MW99381 MW00125 MW00177 MW00151 MW00179 MW98139* MW99507 MW00058 MW99374 MW99393 MW00232 MW99473* MW01039 MW99319 MW99320 MW99471 MW00072 MW00102 MW00476 MW00186 WE02677 WE02675 MW99422 MW99059* MW99449 Fig. 73. COI-Parsimony-Network of Iphiclides 75 Chapter 3 Fig. 74. COI-Parsimony-Network of the P.aedon-group Fig. 75. COI-Parsimony-Network of the Aricia agestis-group 76 A molecular phylogeny of Agrodiaetus Fig. 76. COI-Parsimony-Network of Lysandra 77 Chapter 3 Fig. 77. COI-Parsimony-Network of the Agrodiaetus admetus-group 78 A molecular phylogeny of Agrodiaetus Fig. 78. COI-Parsimony-Network of the Agrodiaetus-menalcas-group 79 Chapter 3 Fig. 79. COI-Parsimony-Network of the Agrodiaetus carmon-group 80 A molecular phylogeny of Agrodiaetus Fig. 80. COI-Parsimony-Network of the Agrodiaetus erschoffii-group 81 Chapter 3 Fig. 81. COI-Parsimony-Network of the Agrodiaetus iphigenia-group 82 A molecular phylogeny of Agrodiaetus Fig. 82. COI-Parsimony-Network of the Agrodiaetus-poseidon-group 83 Chapter 3 Fig. 83. ITS2-Parsimony-Network of Agrodiaetus (grouped) incl. connected outgroups 84 A molecular phylogeny of Agrodiaetus Fig. 84. ITS2-Parsimony-network of Agrodiaetus (excluding outgroups) 85 Chapter 3 Fig. 85. ITS2-Parsimony-Network of Lysandra For the ITS-2 dataset the connection limit was 12 steps for a parsimony probability of 95%. The parsimony analysis of all Polyommatiti ITS-2 sequences with this connection limit produces 8 different networks, if gaps are counted as missing characters. Most taxa, including all taxa of subgenus Agrodiaetus are found in one network. If A. surakovi is excluded from the analysis (due to more than 10% missing character information), A. hamadanensis is disconnected from the carmon-group (Fig. 83). This network includes also most other taxa of the genus Polyommatus. Exceptions are the genus Lysandra (Fig. 85) and Polyommatus thersites which form two separate networks. The genus Agrodiaetus is only connected via Agrodiaetus damon to the outgroup, and the shortest distance is to Polyommatus cornelia (4 steps). A. damon is further connected (with a maximum of 4 steps) to the admetus-group (3 steps), to A. valiabadi (4 steps), the other members of the dolus-group (4 steps) and to A. iphidamon (3 steps). A. iphidamon is the stem species of all other groups which closely resemble the groups found in the combined Bayesian analysis with the following exceptions: A. klausschuriani connects to A. hopfferi in the poseidon-group and A. putnami is not connected to the poseidon-group but to A. iphidamon and to A. birunii in the erschoffii-group. A. glaucias forms a group together with A. tenhageni which is directly connected to A. iphidamon and not to the other members of the erschoffii-group. A. phyllis is also split from the erschoffii-group. The complete network of ITS-2 haplotypes is presented in Fig. 84. A list of collapsed haplotypes can be found in Tab. 11. 86 A molecular phylogeny of Agrodiaetus Tab. 11. Collapsed haplotypes in the ITS-2 network analysis Network Reference specimen code Taxon specimen codes of collapsed haplotypes * taxon different from the reference taxon # reference specimen for further collapsed haplotypes th bold: also collapsed if gaps are coded as 5 character 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 7 7 7 8 AD98012 AD98036 JC00043 MW00051 MW00064 MW00129 MW00176 MW00189 MW00226 MW00231 MW00234 MW00328 MW01001 MW98136 MW98172 MW99009 MW99135 MW99274 MW99372 MW99546 MW99591 JC00029 JC00061 MW00412 MW99537 MW00469 MW01018 MW01092 JC00055 Agrodiaetus altivagans Agrodiaetus phyllis Agrodiaetus ripartii Agrodiaetus elbursicus Agrodiaetus valiabadi Agrodiaetus gorbunovi Agrodiaetus rovshani Agrodiaetus demavendi Agrodiaetus femininoides Agrodiaetus alcestis Agrodiaetus firdussii Agrodiaetus iphidamon Agrodiaetus ainsae Agrodiaetus wagneri Agrodiaetus menalcas Agrodiaetus iphigenia Agrodiaetus turcicus Agrodiaetus dantchenkoi Agrodiaetus baytopi Agrodiaetus damon Agrodiaetus humedasae Polyommatus menelaos Polyommatus andronicus Polyommatus icarus Polyommatus myrrhinus Lysandra bellargus Lysandra coridon Lysandra coridon Aricia artaxerxes MW99353 MW00452 JC00045* MW00110 MW00498 MW00177 WE02662 MW99105 WE02614* MW98212 MW98162* MW99372# MW01039* MW98139 MW99471 MW99309 MW99203 MW99276 WE02491* MW99613 MW99605 JC00042* JC00063* MW00530* MW99550 MW99608 MW01034* MW01116 MW01048* MW99174 JC01014* WE02431* MW00178 MW99196* MW98315 MW99058* MW99164* MW99247 MW99274# MW01053 MW99565 MW99319 WE85001* JC00051 MW01092# In the case of gaps counted as 5th character most Agrodiaetus were also connected in one network, only A. antidolus, A. kurdistanicus and A. femininoides together formed a separate network and three other taxa (A. hamadanensis, A. maraschi and A. paulae) remained unconnected. Discussion Comparisons with traditional systematics The basal splits in the COI gene tree correspond to the traditional classification of Lycaenidae (as far as these are represented in the Palaearctic region) into three subfamilies (Theclinae, Lycaeninae, Polyommatinae) according to ELIOT (1973) or tribes (Theclini, Lycaenini, Polyommatini) according to HESSELBARTH et al. (1995) and support modern views of SCOTT & WRIGHT (1990) and FIEDLER (1991b) that Lycaenini represent the most ancient subdivision of those three taxa. The clades within the tribus Polyommatini also largely follow current subdivisions into subtribes according to HESSELBARTH et al. (1995) and the monophyly of Polyommatiti is supported. Although taxon sampling of outgroups was necessarily coarse, the resolution achieved with the available molecular data was surprisingly good. The only exception appears to be Euphilotes bernardino (Barnes & McDunnough, 1917) which is 87 Chapter 3 sometimes treated as a subspecies of Euphilotes battoides (Behr, 1906). The trees surprisingly indicate that this species should be included in the Polyommatiti (Polyommatus section sensu ELIOT 1973). However, in his original description of the genus Euphilotes, MATTONI (1977) treated this taxon (together with Philotiella) as a relative of Philotes. Species currently assigned to Euphilotes and Philotiella, respectively, had previously been treated under the genus name Philotes, and ever since MATTONI's split all these taxa have been regarded, on the grounds of similarities in male genitalia and wing morphology, as belonging to the Glaucopsyche section sensu ELIOT (1972) (which was later split into two subtribes, Glaucopsychiti and Scolitantiditi, by HESSELBARTH et al. 1995). On the other hand, MATTONI (1977) noted that Euphilotes do differ in a number of points from "true" Philotes, and therefore there is a possibility that traditional morphology-based classification of Euphilotes as members of Scolitantiditi needs to be revised. However, the taxonomic identity of the specimen from which the GenBank sequence AF170864 is derived has not been ascertained so far (F. Sperling pers. comm.), and there remains a slight possibility that a misidentification with the phenotypically very similar Plebeius (= Icaricia) acmon (Westwood & Hewitson, [1852]) has occurred. Thus, unless the identity of this voucher specimen has been crosschecked (which is still in existence, F. Sperling pers. comm.) and in the absence of further supportive data (e.g. presence of an eversible female gonoporus, the best morphological autapomorphy of the true Polyommatiti: HÄUSER 1993), the affiliation of Euphilotes bernardino with the Polyommatiti remains uncertain. Within Polyommatiti the COI and ITS2 gene trees correspond with each other in the exclusion of Cyaniris from a monophyletic genus Polyommatus but not in the position of Lysandra. While the COI gene tree which places Lysandra within Polyommatus confirms current morphology-based systematics, its basal placement within Polyommatiti in the ITS2 gene tree might be caused by alignment problems in the most distant groups and the high divergence of Lysandra sequences due to an apparent faster evolutionary rate, which might be caused by microsatellites. Disagreement in the COI and ITS2 data sets occur mostly in the less well resolved parts of the tree. The combined tree provides the best resolution within Agrodiaetus which proves that both data sets complement each other. The resulting clades obtained from the MP and Bayesian phylogenetic analysis are largely concordant with each other, but do not correspond very well with current groupings (HESSELBARTH et al. 1995; BÁLINT & JOHNSON 1997; ECKWEILER & HÄUSER 1997). However, these traditional, morphology-based approaches are also far from being congruent. In Tab. 12 the new subdivisions based on molecular phylogenetic data are contrasted with the three most recent conventional classification schemes. Tab. 12. Agrodiaetus-clades on the basis of molecular data in comparison to previous morphology-based grouping concepts Agrodiaetus-clades on the basis of molecular data n Hesselbarth Bálint & et al. (1995) Johnson (1997) Eckweiler & Häuser (1997) 45 damon damon damon admetus admetus admetus admetus admetus admetus admetus admetus admetus admetus admetus admetus admetus damon-group damon ([Denis & Schiffermüller], 1775) admetus-group admetus (Esper, [1783]) ripartii (Freyer, 1830) nephohiptamenos (Brown & Coutsis, 1978) demavendi (Pfeiffer, 1938) khorasanensis (Carbonell, 2001) lorestanus Eckweiler, 1997 88 78-80 90 ca.90 66-76 84 69-74 admetus A molecular phylogeny of Agrodiaetus Agrodiaetus-clades on the basis of molecular data n Hesselbarth Bálint & et al. (1995) Johnson (1997) Eckweiler & Häuser (1997) dolus dolus admetus admetus admetus dolus admetus admetus admetus admetus admetus dolus admetus admetus dolus dolus admetus admetus admetus dolus admetus admetus admetus admetus damon carmon damon damon transcaspicus damon damon damon damon damon dolus-group ainsae (Forster, 1961) fulgens (de Sagarra, 1925) fabressei (Oberthür, 1910) humedasae (Toso & Baletto, 1976) aroaniensis (Brown, 1976) menalcas (Freyer, [1837]) alcestis (Zerny, 1932) interjectus (de Lesse, 1960) dantchenkoi (Lukhtanov & Wiemers, 2003) valiabadi (Rose & Schurian, 1977) 108-110 103 90 38 48 85 19-21 29-32 40-42 23 iphidamon-group iphidamon (Staudinger, 1899) dizinensis (Schurian, 1982) 14 17 carmon carmon-group ninae (Forster, 1956) turcicola (Koçak, 1977) huberti (Carbonell, 1993) zapvadi (Carbonell, 1993) elbursicus (Forster, 1956) paulae (Wiemers & De Prins, 2003) arasbarani (Carbonell & Naderi, 2000) zarathustra Eckweiler, 1997 pierceae (Lukhtanov & Dantchenko, 2002) carmon (Herrich-Schäffer, [1851]) schuriani (Rose, 1978) surakovi Dantchenko & Lukhtanov, 1994 sekercioglui (Lukhtanov & Dantchenko, 2002) hamadanensis (de Lesse, 1959) theresiae Schurian, van Oorschot & van den Brink, 1992 guezelmavi Olivier, Puplesiene, van der Poorten, De Prins & Wiemers, 1999 dama (Staudinger, 1892) karindus (Riley, 1921) peilei Bethune-Baker, 1921 femininoides (Eckweiler, 1987) morgani (Le Cerf, 1909) kurdistanicus (Forster, 1961) antidolus (Rebel, 1901) 33-37 19-20 33-37 18-19 16-18 17 ca. 22 22 81-82 81-82 50 50 21-22 63 transcaspicus transcaspicus carmon transcaspicus transcaspicus transcaspicus damon carmon carmon damon damon damon carmon transcaspicus dama dama dama dama poseidon dama dolus dolus dolus dolus dolus dolus dolus dolus dama dama dolus dolus dolus dolus dolus erschoffii erschoffii 42 41 66-68 39 27 25-27 57-62 40-41 erschoffii-group erschoffii (Lederer, 1869) achaemenes Skala, 2002 shahrami Skala, 2001 klausschuriani Ten Hagen, 1999 tenhageni Schurian & Eckweiler, 1999 phyllis (Christoph, 1877) glaucias (Lederer, 1871) darius Eckweiler & Ten Hagen, 1998 caeruleus (Staudinger, 1871) posthumus (Christoph, 1877) birunii Eckweiler & Ten Hagen, 1998 13-15 128-131 56 78-82 damon dolus glaucias damon erschoffii 20 ca. 85 10-11 poseidon damon transcaspicus damon damon damon 65-67 poseidonides iphigenides 24 erschoffii poseidonides dagmara iphigenides iphigenides-group iphigenides (Staudinger, 1886) poseidonides-group dagmara (Grum-Grshimailo, 1888) poseidonides (Staudinger, 1886) 89 Chapter 3 Agrodiaetus-clades on the basis of molecular data Hesselbarth Bálint & et al. (1995) Johnson (1997) Eckweiler & Häuser (1997) 12-16 24 27 damon carmon damon 20-21 29 damon damon damon carmon damon damon damon damon damon damon damon damon damon damon 19-22 25-27 15 21-22 18 29 24-32 17 21-22 25-29 poseidon poseidon poseidon poseidon damon poseidon poseidon n iphigenia-group iphigenia (Herrich-Schäffer, [1847]) turcicus (Koçak, 1977) baytopi (de Lesse, 1959) rovshani Dantchenko & Lukhtanov, 1994 tankeri (de Lesse, 1960) iphicarmon Eckweiler & Rose, 1993 poseidon-group poseidon (Herrich-Schäffer, [1851]) putnami (Lukhtanov & Dantchenko, 2002) hopfferi (Herrich-Schäffer, [1851]) lycius (Carbonell, 1996) ernesti Eckweiler, 1989 pseudactis (Forster, 1960) firdussii (Forster, 1956) actis (Herrich-Schäffer, [1851]) artvinensis (Carbonell, 1997) sigberti Olivier, van der Poorten, Puplesiene & De Prins, 2000 haigi (Lukhtanov & Dantchenko, 2002) mithridates (Staudinger, 1878) mofidii (de Lesse, 1963) altivagans (Forster, 1956) kanduli (Lukhtanov & Dantchenko, 2002) merhaba De Prins, van der Poorten, Borie, Oorschot, Riemis & Coenen, 1991 wagneri (Forster, 1956) maraschi (Forster, 1956) sertavulensis (Koçak, 1979) pseudoxerxes (Forster, 1956) sennanensis (de Lesse, 1959) gorbunovi Dantchenko & Lukhtanov, 1994 cyaneus (Staudinger, 1899) 21-25 21-27 34-35 18-23 25 16-17 16-18 16 20 15-16 28-30 19-20 16-20 actis actis actis actis actis actis damon damon damon damon damon damon damon dolus damon actis admetus dolus actis dolus damon damon carmon carmon damon actis actis actis carmon dolus actis damon damon damon damon dolus damon damon carmon carmon dolus actis carmon The ITS-2 network analysis supports the larger clades found in the COI and combined cladograms but also provides evidence that the bad resolution in some closely related species groups and the peripheral position of certain taxa like A. iphidamon and A. iphigenides in the cladograms is due to the survival of stem species. A. damon appears to be directly derived from the ancestor of all Agrodiaetus and A. iphidamon the stem species for the iphigenides-, iphigenia-, erschoffii-, poseidon-, carmon- and poseidonides-clades. The short distances between the stem species and the subnetworks provide evidence for the fast radiation of Agrodiaetus. Phylogenetic relationships within the genus Agrodiaetus are discussed in more detail in Chapter 4. Congruence between gene trees and species trees in outgroups If relationships between closely related species are discussed the question of congruence between gene trees and species trees arises. If both are identical, species (if defined as reproductively isolated units) should form monophyletic groups in the cladograms. Apparently some exceptions can be found, one of the most striking examples being Polyommatus icarus (Rottemburg, 1775). While populations from Spain to Iran appear as a monophyletic group (including the Greek Polyommatus andronicus Coutsis & Ghavalas, 1995 which has been separated only recently from P. icarus based on disputable evidence), the Moroccan specimen of P. icarus is placed outside this clade. This result came as a surprise 90 A molecular phylogeny of Agrodiaetus because Northwest African populations of P. icarus are thought to represent the same subspecies as in Europe (TENNENT 1996). The COI and ITS-2 p-distances between the Moroccan and Eurasian populations of P. icarus differ to a much higher degree than in other species (Tab. 13), including those with well differentiated subspecies in Northwest Africa (like Polyommatus amandus), and are on the level of well differentiated species. The Moroccan specimen also differs in phenotype from the other icarus (f. celina Austaut), but P. icarus is an extremely variable species throughout its vast, trans-Palaearctic distributional range. Without further material it cannot be decided if Northwest African populations of P. icarus are so divergent from Eurasian ones that they should better be seen as representing a distinct Polyommatus species. Tab. 13. P-distances between Moroccan and Eurasian populations Species Country comparison p-distance COI p-distance ITS-2 Aricia montensis Celastrina argiolus Cyaniris semiargus Iphiclides feisthamelii Iphiclides feisthamelii - podalirius Lampides boeticus Lampides boeticus Lycaena alciphron Polyommatus amandus Polyommatus icarus Polyommatus icarus Polyommatus icarus Polyommatus icarus Polyommatus icarus Polyommatus andronicus - icarus Morocco-Spain Morocco-Turkey Morocco-Iran Morocco-Spain Spain - Greece Morocco-Spain Morocco-Turkey Morocco-Iran Morocco-Turkey Morocco-Spain Morocco-Greece Morocco-Turkey Morocco-Iran Spain-Iran Greece 0.006 0.013 0.012 0.021 0.003 0.003 0.002 0.027 0.037 0.068 0.063 0.059 0.060 0.012 0.007 0.005 0.011 0.001 0.007 0.025 0.017 0.015 0.017 0.000 Another surprise involving a Moroccan taxon was found in the species pair Iphiclides podalirius – feisthamelii (Papilionidae). The latter replaces the former in Northwest Africa and the Iberian Peninsular. Opinions differ whether these two taxa represent different species or just subspecies and no detailed studies are known from the contact zone in the French Pyrenees. The COI haplotype of I. feisthamelii (Duponchel, 1832) from Spain is identical to the I. podalirius (Linnaeus, 1758) sequence AF170873 in GenBank and very similar to Greek I. podalirius, but Moroccan and Spanish I. feisthamelii sequences are very different from each other (Tab. 13, Fig. 73). Although it cannot be ruled out that the GenBank sequence represents I. feisthamelii, because the specimen is from “France” where both taxa occur and the voucher specimen is unavailable (Sperling, pers. comm.), the COI data indicate a high level of differentiation between Northwest African and European populations but not between the two taxa feisthamelii and podalirius. Further cases where the gene trees do not seem to correspond with taxonomical species catagories are found outside Agrodiaetus in the following species groups: Aricia agestisartaxerxes, Polyommatus eros-eroides-menelaos, Meleageria daphnis-marcida, Polyommatus aedon-myrrhinus-cornelia, and the genus Lysandra. Aricia agestis (Denis & Schiffermüller, 1775) and A. artaxerxes (Fabricius, 1793) are very closely related species which are known to interbreed (AAGARD et al. 2002; HOEGHGULDBERG 1979, 1982). The two Greek specimens of Aricia artaxerxes had a very different 91 Chapter 3 COI haplotype, one of them (JC00057) almost identical to Aricia agestis. This might be due to mtDNA introgression because these specimens were found sympatrical with A. agestis on Mt. Taiyetos. The Polyommatus eros-eroides complex consists of mostly allopatric populations whose relationships are unclear. P. menelaos Brown, 1996 from Peleponnesos is thought to be closely related to P. eros Ochsenheimer, 1808 by most authors and not to P. eroides Frivaldszky, 1835 which is found in mainland Greece, but the status of P. eroides and P. eros as distinct species is only founded on very slight differences in coloration and in habitat, with P. eros inhabiting mainly higher altitudes above 1800 m. Although the total ranges of both taxa overlap they are not known to occur in sympatry. P. eroides forsteri Pfeiffer, 1938 from Elburs Mts. in Iran is treated as a distinct species by BÁLINT (1993) and CARBONELL (1994b). The taxon yildizae Kocak, 1977 from Kop Dağı Mts. in Turkey was described as a subspecies of P. eroides and treated as such by CARBONELL (1994b) but transferred to P. forsteri by BÁLINT (1993) and to P. eros by HESSELBARTH et al. (1995) even though they noticed that the distribution of this taxon between two subspecies of P. eroides is hard to explain on zoogeographic grounds. Different species boundaries are again suggested by TUZOV et al. (2000a) who combine high altitude populations from Azerbaijan, Iran and Turkey (ssp. moletti Carbonell, 1994) under the name Polyommatus erotulus Nekrutenko, 1985, whereas GORBUNOV (2001) recognizes even five units in this species complex. Available genetic data only support a division between Iranian forsteri on one side and Greek/Turkish eroides/menelaos/yildizae on the other side which agrees best with CARBONELL (1994b). Nominotypical P. eros (from the Alps) have not been investigated though. Meleageria marcida (Lederer, 1872) is a discoloured variety of Meleageria daphnis (Denis & Schiffermüller, 1775) confined to the Northern slopes of Elburs Mts. in Iran which was raised to species rank by BÁLINT & JOHNSON (1997) although hybrids with intermediate phenotype are known (SCHURIAN 1989b). The discoloration is probably an adaption to the specific climatic conditions (low solar radiation) on the north side of Elburs Mts. (BIRO et al. 2003). The COI sequence of M. marcida from Veresk is very different from the other daphnis populations, but another specimen from Kendevan Pass has a haplotype similar to daphnis. This suggests that M. marcida is well differentiated genetically, but gene flow exists in contact zones (one of which is near Kendevan Pass). Polyommatus myrrhinus (Staudinger, 1901) from Eastern Anatolia was described as a subspecies of Polyommatus myrrha (Herrich-Schäffer, [1851]) which is found in other parts of Anatolia. HESSELBARTH et al. (1995) ranked it as a subspecies of Polyommatus aedon (Christoph, 1887) from Elburs Mts. (Iran) whereas BÁLINT & JOHNSON (1997) treated it as a distinct species. The large genetic distance in the COI data supports the specific distinctness of myrrhinus and aedon but does not exclude the possibility that P. myrrhinus is conspecific with true P. myrrha which was not analyzed. The COI and ITS2 haplotypes of Polyommatus cornelia (Gerhard, [1850]) are extremely similar to P. myrrhinus. Although both taxa are sometimes united in the subgenus Sublysandra (e.g. BÁLINT & JOHNSON 1997) they are quite different in wing pattern and definitely constitute different species which occur sympatrically in Anatolia without hybridization. The molecular data set implies that these taxa are very young and therefore not well differentiated genetically. It does not agree well with the conclusions of FIEDLER et al. (1994) drawn from the study of life history parameters which suggest a closer relationship of P. cornelia (= P. candalus (Herrich-Schäffer, [1851]) to P. icarus than to the P. myrrha-complex. It should be noted that P. cornelia is a very variable butterfly (HESSELBARTH et al. 1995) and karyological data (DE LESSE 1960a) indicate that it might comprise different species. 92 A molecular phylogeny of Agrodiaetus The subgenus Lysandra is a group of closely related taxa some of which are known to interbreed with each other (SCHURIAN 1989b & 1989c). Many taxa and allopatric populations differ in chromosome numbers (DE LESSE 1960a, 1969) which range between n=24 in Lysandra syriaca (Tutt, [1910]) and n=92 in populations of Lysandra coridon (Poda, 1761) from the Balkan Peninsula. Because of its plesiomorphic chromosome number, L. syriaca (from Lebanon and South Turkey) is thought (SCHURIAN 1989b) to represent the most basal Lysandra (together with L. punctifera (Oberthür, 1876) from Morocco). The COI data confirm this hypothesis. L. bellargus (Rottemburg, 1775) (n=45) which has an extensive distribution from Spain to Iran is found sympatrically with most other Lysandra taxa. Hybrids with L. coridon have sometimes been found in nature (polonus Zeller, 1847), but F1 hybrids are probably sterile and extensive hybridization experiments with L. coridon, L. hispana and L. ossmar have failed (SCHURIAN, 1989b). The COI and ITS2 data confirm the monophyly of L. bellargus populations from Spain, Italy, Turkey and Iran without indications of gene flow with other Lysandra species. L. ossmar (Gerhard, 1851) and L. corydonius (Herrich-Schäffer, 1852) are thought to be sister species differing in the upperside wing colour (grey vs. blue) which are parapatrically distributed in West and Central Anatolia (L. ossmar) and Eastern Anatolia to Caucasus and Transcaucasus (L. corydonius). Their chromosome numbers are identical (n=84), but L. ossmar has two large chromosomes compared to three in L. corydonius (DE LESSE 1969). Natural hybrids are known from the contact zone in Sivas, Erzincan and Erzurum Province. SCHURIAN (1989b) managed to breed such hybrids until the F3-generation. The ITS2 gene tree conforms to the supposed species tree, but the sequences are very similar and only two positions are parsimony-informative. In the COI gene two different groups of haplotypes can be identified which differ in five positions, but the specimens from Sivas and Erzurum (near/in the contact zone) possess the haplotypes of the opposite species which causes the difference of the COI gene tree from the species tree. Although more material would be needed to clarify this situation, the data indicate extensive mtDNA introgression across the contact zone. (The two specimens from Erzurum have the same haplotype apart from two positions, one of which is identical to L. coridon from Italy in MW99042. This specimen also has missing character information at 16% of all COI nucleotides which causes the odd placement with L. coridon in the Bayesian analysis. The MP analysis seems to be less sensitive to missing character information and correctly places the two corydonius from Erzurum together.) The remaining Lysandra taxa belong to the Lysandra coridon species complex which has been the main focus of LELIEVRE’s (1992) study of allozymes in this group. Lysandra coridon (Poda, 1761) is the most widespread species which is found from Northern Spain (ssp. asturiensis De Sagarra, 1924) through Central Europe (type locality: Graz in Austria) to Greece. Allozyme variation indicates a division between a western and eastern group of populations (SCHMITT & SEITZ 2001). The chromosome numbers increase gradually from n=87 in Southwest Europe (Spain and Italy) to n=92 in Southeast Europe (Macedonia) (DE LESSE 1969). The allopatric taxa caelestissimus Verity, 1921 from Montes Universales (Central Spain) and gennargenti Leigheb, 1987 from Sardinia are thought to be subspecies of L. coridon by most authors but treated as distinct species by KUDRNA (2002). LEIGHEB (1987) suggested a close relationship between gennargenti and caelestissimus due to similarities in wing coloration. Allozyme variation however indicates that gennargenti populations originated from the Italian mainland and differentiation is due to gene drift and inbreeding (MARCHI et al. 1995). Lysandra albicans (Gerhard, 1851) with a chromosome number of n=82 replaces L. coridon in Central and Southern Spain. Hybrids with L. caelestissimus have often been found and even named (caerulescens Tutt) and supposed hybrids with L. coridon asturiensis are known from the contact zone (e.g. near Peñahorada/Burgos; LELIEVRE, 1992). Specimen MW01018 is from the same locality and looks like the specimen figured as possible hybrid in LELIEVRE (1992: 93 Chapter 3 photo 2, fig. 16). The picture is further complicated by the fact that another species, Lysandra hispana (Herrich-Schäffer, 1852) with n=84 chromosomes, occurs in coastal regions from Catalonia to Tuscany. The 2nd generation of this bivolitine species can occur together with the only generation of L. albicans and L. coridon and can not be reliably separated at these locations (SCHURIAN, 1989b). L. coridon and L. hispana have been crossed very successfully until the F3 generation (BEURET 1956-1959). It is possible that the specimens from Sta. Coloma de Queralt (Tarragona) represent this taxon. The ITS2- and COI-sequences from the taxa analyzed in the Lysandra coridon-complex form one monophyletic clade which confirms that they are not well differentiated genetically. Populations of L. albicans are placed at basal positions in the tree and in the network which might indicate that L. albicans is the ancestral taxon, but the material is not sufficient for far-reaching conclusions. Comparison with allozyme results The only study of allozymes in Agrodiaetus was published by MENSI et al. (1994) who investigated some monomorphic Agrodiaetus species together with Agrodiaetus damon ([Denis & Schiffermüller], 1775). Unfortunately the chromosome number of the specimens used was not determined and therefore the identification of several taxa is doubtful, especially those from Anatolia (A. ripartii, A. demavendi and A. interjectus). A. interjectus is not known from Van Province, but four other similar karyospecies occur there: A. dantchenkoi, A. alcestis, A. demavendi and A. ripartii. Despite these flaws, some interesting comparisons with the DNA results are possible. Allozyme and DNA results correspond in the following points: • A. damon forms a distinct clade from the other investigated Agrodiaetus species. • The monomorphic Agrodiaetus species (A. fabressei, A. humedasae, A. admetus, A. ripartii) are closely related and form one clade together with the dimorphic taxa A. menalcas, A. dolus and A. fulgens. • A. admetus and A. ripartii are very closely related (possibly sister species). The only differences are in the position of A. fabressei and A. humedasae which cluster within the dolus-group in the DNA analysis but occupy an ancestral position in the allozyme studies. Of interest is also the position of two taxa which were not included in the DNA study: • A. dolus (Hübner, 1823) from South France appears closely related to A. fulgens and A. menalcas, with A. dolus vittatus (Oberthür, 1892) from Aveyron more closely related to A. fulgens than to A. dolus dolus (Bouches du Rhône). This result would question the status of A. fulgens as a distinct species. • A. exuberans (Verity, 1926), a very local endemic from Oulx (Torino, Italy) appears to be an ancestral taxon, closely related to A. fabressei and A. humedasae. Hybridization in Agrodiaetus Despite the presumed close relationships between many Agrodiaetus species, not much is known about hybridization in this subgenus of Polyommatus. In contrast to the related subgenus Lysandra, where extensive hybridization experiments have been conducted (SCHURIAN 1989b), the only available evidence for hybrids in Agrodiaetus so far stems from a few collected specimens which are thought to represent hybrids due to their intermediate phenotype. The following natural hybrids (all of them males) have been recorded: • within Agrodiaetus: o A. ripartii (Freyer, 1830) x damon ([Denis & Schiffermüller], 1775) (SCHURIAN & HOFMANN 1975) o A. ripartii x menalcas (Freyer, [1837]) (SCHURIAN & HOFMANN 1980) o A. antidolus aereus Eckweiler, 1998 x (?) cyaneus (Staudinger, 1899) (TEN HAGEN 2003) 94 A molecular phylogeny of Agrodiaetus • of Agrodiaetus with other Polyommatus subgenera: o A. damon x Meleageria daphnis ([Denis & Schiffermüller], 1775) (REBEL 1920) o A. damon x Lysandra coridon (Poda, 1761) (REBEL 1930a) o A. damon x Polyommatus icarus (Rottemburg, 1775) (REBEL 1930b) o A. ectabanensis x Meleageria daphnis elamita (Le Cerf, 1913) (TEN HAGEN 2003) o A. turcicus (Koçak, 1977) x Polyommatus icarus (TEN HAGEN 2003) The problem with such records is that the conclusions about the parental species of those natural hybrids (which might also be just aberrations) are very uncertain. In the case of the presumed hybrid ripartii x menalcas, at least one other karyospecies similar to A. ripartii, A. alcestis (Zerny, 1932), probably occurs at the site at Zelve (Nevşehir Prov., Turkey). Obviously, hybrids between phenotypically similar species would usually be overlooked (the same is true for any female hybrid within Agrodiaetus) or recorded as a variation of one of the parental species, and only hybrids between very different phenotypes are likely to be discovered. During the expeditions to Turkey, Iran, Italy and Spain, only one specimen was found which appeared to be a hybrid because of its intermediate phenotype. This male specimen (MW99471) from Erek Dağı (Van Prov., Turkey) has brown wings with traces of silvery scales which would be expected in a hybrid between a species of the “brown” Agrodiaetus complex and one with silvery males. Possible parental species were two species with silvery males, A. menalcas (Freyer, [1837]) and A. kurdistanicus (Forster, 1961), which were both found at the site, and four karyospecies with brown males which are known to occur in Van Province, A. alcestis, A. dantchenkoi (Lukhtanov & Wiemers, 2003), A. demavendi (Pfeiffer, 1938) and A. ripartii. Mitochondrial DNA is maternally inherited and would therefore indicate the mother species of the presumed hybrid. In this case, the mtDNA COI sequence turned out to be identical to those of two specimens of A. dantchenkoi from nearby Kurubaş Geçidi. In nuclear genes there is a 50% chance that either the paternal or maternal copy is inherited. The ITS-2 sequence was identical to the sequence of A. menalcas including the ‘A’ in ITS-2 position 613 in the aligned data set which was not found in any other Polyommatiti sequence. Thus it can be concluded that specimen MW99471 is a hybrid between a female of A. dantchenkoi which has a chromosome number of n=40-42 and a male of A. menalcas which has the double chromosome number of n=85. The chromosome number of the hybrid specimen is approximately the same as in A. dantchenkoi and this would be expected in an F1 hybrid if a complete pairing between each dantchenkoi chromosome with two menalcas chromosomes is achieved by trivalent formation in meiosis whereas intermediate chromosome numbers usually occur in Fn and backcrosses. Chromosome numbers in hybrids can also be higher than those of their parents if no complete pairing is achieved. A. dantchenkoi and A. menalcas appear to be very closely related and might even be sister species and the occurrence of a hybrid between them is therefore hardly surprising. Meiotic behaviour of chromosomes in hybrids of two closely related Lepidoptera species with a different karyotype, Antheraea roylei (n=31) and A. pernyi (n=49), was studied by NAGARAJU & JOLLY (1986) who observed the formation of 18 trivalents + 13 bivalents (n=31) in F1 hybrids and two different karyotypes with either 49 bivalents or 9 trivalents + 31 bivalents (n=40) in backcrosses. A comparison of mtDNA and nuclear sequences did not reveal any other hybrid in Agrodiaetus. Although hybrids between very closely related species (such as those with identical nuclear or mtDNA sequences) can not safely be detected in this way, the existence 95 Chapter 3 of hybrids between distantly related species, especially those from different species groups within the studied material can firmly be excluded. Radiation of Agrodiaetus and the colonization of Europe Genetic data confirm current opinions (e.g. HESSELBARTH et al. 1995) that Agrodiaetus is a very young radiation originating in the Pliocene, with most intense speciation during the Pleistocene. The biogeographical origin of the subgenus Agrodiaetus remains unsolved, because the sister group within Polyommatus could not be exactly determined and A. damon, a close relative of the ancestor species of Agrodiaetus, has an extremely extensive distribution from the Pyrenees to Mongolia, where it is confined to isolated mountain ranges like most other Agrodiaetus species. The lower altitudinal limits of most Agrodiaetus increase from the West (Spain: 500 m) to the East (Iran: 1500 m), but are usually around 1000 m. Only few species are found in the lowlands or even near sea level (e.g. A. admetus) and some are even confined to very high altitudes (e.g. A. faramarzii Skala, 2001 at 4000 m in the Zagros range of Iran). It can be assumed that A. damon had an almost continuous distribution at the end of the Pleistocene (Dryas 10000 years ago) but populations retreated into the mountains when the climate warmed up and became isolated from each other. Obviously the centre of Agrodiaetus radiation is Eastern Anatolia, Transcaucasia and Iran. Not only do these regions have the highest species diversity, but species of these regions are present in almost all species groups (with the only exception of some Central Asian ones) and their stem species are also found in these areas, most notably A. iphidamon from Elburs Mts. in northern Iran. The orogeny enabled them to evade even severe climate changes by moving up and down the mountains without the need of long range dispersal. Many species are now confined to isolated mountain tops with subalpine or alpine Onobrychis steppe and have a discontinuous distribution, but during at least ten arctic periods of the Pleistocene these populations were able to extend their distribution and to intermix, but were separated again during the interstadials, thus producing complicated speciation patterns. Europe was only reached by few Agrodiaetus species which belong to the dolus-, admetusand iphigenia-clade. European endemic (karyo-)species are only found in the dolus-clade and therefore this clade probably represents the first European radiation. The ancestor species is a close relative of the three Anatolian species A. dantchenkoi, A. interjectus and A. alcestis. Relicts of this first colonization are the monomorphic species A. fabressei (Central Spain), A. humedasae (Aosta, Italy), and A. aroaniensis (Greece) which appear to have survived the last glaciation in close proximity to their current occurrence. The Southwest Mediterranean A. dolus, A. ainsae and A. fulgens appear very close genetically to A. fabressei (nuclear and mtDNA) but also to the phenotypically more similar Anatolian A. menalcas (allozymes, v. MENSI et al. 1994), thus their origin remains unclear. The admetus-clade represents a distinct colonization including A. ripartii which is widely distributed in the Mediterranean region (Northern Spain, Southern France, Balkans) through Anatolia and Russia to Altai and the closely related Anatolian A. admetus which adapted especially well to low altitudes and occurs throughout the Balkan peninsula. The only species with blue-coloured males (apart from A. damon) that got a foothold in Europe is the Anatolian A. iphigenia which marginally extended its distribution to Southern Greece during the Pleistocene and is now restricted in Europe to Mt. Chelmos in the Peleponnesos. 96 Systematics of Agrodiaetus Chapter 4: Systematics of Agrodiaetus based on molecular evidence – a new perspective The following systematic list attempts to summarize the molecular results to infer the relationships between species of the subgenus Agrodiaetus taking into account the available evidence from karyological, morphological and biogeographic studies. It includes only those taxa which were included in the molecular study. damon-group This group contains only one species with a most extensive distribution from Spain to Mongolia. It represents the sister of all other Agrodiaetus according to the nuclear DNA data set. damon ([Denis & Schiffermüller], 1775) Despite its vast distribution the genetic variation appears small. Samples from the French Alps and from NE Turkey are genetically very similar. The sister taxon of A. damon remains unclear. iphidamon-group This ancestral group consists of two closely related species confined to the Iranian Elburs Mountains. iphidamon (Staudinger, 1899) This species represents a genotype that is close to the presumed ancestor of all species groups with blue males (iphigenides-, iphigenia-, erschoffii-, carmon-, actis- and poseidonidesgroup). The nuclear ITS-2 sequences are identical with possible stem species in the iphigeniagroup (A. baytopi) and erschoffii-group (A. shahrami & A. achaemenes). dizinensis (Schurian, 1982) This local endemic which is only known from the type locality Dizin (Central Elburs Mts.) appears to be most closely related to A. iphidamon. ECKWEILER & HÄUSER (1997) suggest a close relationship to A. kendevani (Forster, 1956), a taxon which could not be included in this study. iphigenides-group A Central Asian species group of which only one species was sampled. iphigenides (Staudinger, 1886) poseidonides-group This Central Asian group appears very distant from the other groups. Study of more Central Asian taxa is necessary to decide if e.g. it can be united with the iphigenides-group. poseidonides (Staudinger, 1886) In the ITS-2 network analysis this taxon seems to be most closely related to A. iphigenides. dagmara (Grum-Grshimailo, 1888) Genetically this taxon is closely related to A. poseidonides. 97 Chapter 4 iphigenia-group Small Anatolian species group. Possible ancestor species: A. iphidamon. The following four taxa with mostly allopatric distribution appear to be very closely related genetically. baytopi (de Lesse, 1959) The ITS-2 sequence of this East Anatolian species is identical to the suggested ancestor species iphidamon and therefore it could be the stem species of the iphigenia-group. tankeri (de Lesse, 1960) This taxon differs from the previous species in phenotype and karyotype and is distributed parapatrically in NE Turkey while A. baytopi is found in SE Turkey. A sympatric occurrence is known from Tahir Geçidi (Ağrı Prov., Turkey; HESSELBARTH et al. 1995). ITS-2 sequences of both taxa are identical and their mtDNA haplotypes overlap which raises the question if speciation is complete or if gene flow persists. rovshani Dantchenko & Lukhtanov, 1994 This taxon whose karyotype remains unknown occurs in Azarbaijan and Iranian Azarbaijan and is genetically close to A. baytopi and A. tankeri. iphicarmon Eckweiler & Rose, 1993 Genetic data confirm the karyological results that this taxon is not a subspecies of A. iphigenia but instead closely related to A. baytopi. turcicus (Koçak, 1977) This Eastern Anatolian species is also closely related to A. baytopi and A. tankeri but occurs sympatrically with them. iphigenia (Herrich-Schäffer, [1847]) This is the only representative of this group whose range extends to Europe (Peleponnesos, Greece). Populations from different parts of Anatolia and Armenia are genetically very similar and well differentiated from the other members of the iphigenia-group, which are often found sympatrically. admetus-group This group has an extensive distribution from Northern Spain to Kazakhstan and includes only monomorphic brown species with an elevated number of chromosomes (n=65-90). The ancestor species of this group seems to be a close relative of A. damon. The following three taxa are karyospecies with an allopatric distribution, but indistinguishable on morphological grounds. ripartii (Freyer, 1830) Despite of their similarities in phenotype and karyotype populations of this widespread taxon from Spain, Greece and Turkey do not form a monophyletic group which could be taken as an indication that A. ripartii represents the stem species for the remaining taxa in this group. Alternatively it is possible that speciation is incomplete and gene flow between the karyospecies A. ripartii and A. demavendi persists. khorasanensis (Carbonell, 2001) This taxon from Kopet Dagh (Iran) with a chromosome number intermediate between the previous and the following taxon is genetically close to A. ripartii. demavendi (Pfeiffer, 1938) Genetic and karyological data indicate that this karyospecies might consist of several species with only slightly different chromosome numbers but further investigations which include the precise determination of chromosome numbers are necessary. Dugijan (Azarbaijan-e Sharqi, Iran) turned out to be one interesting location where two different mtDNA haplotypes were found which might indicate the coexistence of two different species. These haplotypes were both found from Eastern Turkey to Northwest Iran. Slight differences in phenotype have led to recent descriptions of new species like A. ahmadi (Carbonell, 2001) and A. urmiaensis 98 Systematics of Agrodiaetus (Schurian & ten Hagen, 2003), unfortunately without karyological data, and it is uncertain whether the recorded differences can be used to delimit species. lorestanus Eckweiler, 1997 This taxon was described as a subspecies of A. demavendi and its karyotype is probably identical (Carbonell, 2001). Genetic data confirm the close relationship to A. demavendi. nephohiptamenos (Brown & Coutsis, 1978) This taxon from Macedonia might constitute a synonym of A. ripartii unless it can be confirmed that is has a different karyotype. Genetically and phenotypically it appears to be very close to A. ripartii. admetus (Esper, [1783]) This species has an extensive distribution from the Balkans to Western Siberia and occurs sympatrically with most of the former taxa. Genetically it is closely related to A. ripartii and A. demavendi. dolus-group This is a group of taxa with brown or whitish males which is distributed from Southern Spain to Elburs Mts. (Iran) and Lebanon. It corresponds to the menalcas-group which resulted from the Bayesian analysis of COI and ITS-2. The ancestor species of this group seems to be a close relative of A. damon. valiabadi (Rose & Schurian, 1977) This species appears to be the most ancestral in the dolus-group. dolus (Hübner, [1823]) Material of this taxon from Southern France with whitish males could not be included in this study, but the following two taxa are apparently very closely related. ainsae (Forster, 1961) This is the Spanish representative of A. dolus which differs only slightly in chromosome numbers. fulgens (de Sagarra, 1925) DNA sequences of this taxon are identical to A. ainsae which further questions its status as a distinct allopatric species (see Chapter 2). fabressei (Oberthür, 1910) Despite the similar phenotype and karyotype this taxon does not seem to be closely related to ripartii. Instead it appears to be very closely related to A. ainsae to which it is allopatric in distribution (MUNGUIRA et al. 1995). interjectus (de Lesse, 1960) The comparison of nuclear and mtDNA confirms DE LESSE’s opinion (1960b) that this taxon is not a hybrid species between A. alcestis and A. demavendi or A. ripartii despite of its intermediate chromosome number but instead it is genetically very close to A. alcestis. It occurs sympatrically with A. alcestis and A. demavendi. dantchenkoi (Lukhtanov & Wiemers, 2003) This karyospecies is closely related to A. interjectus to which it is allopatric in distribution. alcestis (Zerny, 1932) The two subspecies with slightly different karyotypes, the nominate one from Lebanon and Anatolia and ssp. karacetinae Lukhtanov & Dantchenko, 2002 from Kordestan have similar ITS-2 sequences but different and independently evolved COI haplotypes indicating possible specific distinctness of these two taxa. menalcas (Freyer, [1837]) Although this Anatolian taxon has whitish males like A. dolus/ainsae/fulgens it appears to be more closely related to A. alcestis. aroaniensis (Brown, 1976) This Greek taxon also appears most closely related to A. alcestis. humedasae (Toso & Baletto, 1976) This Italian endemic from Aosta valley is very closely related to A. aroaniensis. 99 Chapter 4 erschoffii-group This is a predominantly Iranian group with one species reaching Central Anatolia. Most members of this group appear closely related to their ancestor species A. iphidamon. shahrami Skala, 2001 This high mountain endemic from the Zagros range appears to be one of the most ancestral because its ITS-2 sequence is identical to A. iphidamon. achaemenes Skala, 2002 A very close relative of A. shahrami with almost identical sequences. It is an allopatric endemic of another high mountain in the Zagros range. phyllis (Christoph, 1877) This species which ranges from Elburs Mts. to Central Anatolia represents a monophyletic group of populations which are well differentiated genetically from all other members of this group. glaucias (Lederer, 1871) The closest relative of this taxon which was described from Elburs Mts. (Iran) appears to be A. birunii. tenhageni Schurian & Eckweiler, 1999 This taxon from Khorasan (Iran) appears to be closely related to the ancestor species A. iphidamon. klausschuriani ten Hagen, 1999 The origin of this species remains unclear. In the ITS-2 network analysis it connects with the actis-group. posthumus (Christoph, 1877) This taxon appears to be restricted to the eastern Elburs Mts. The genetic analysis confirms the specific distictness from A. phyllis which occurs sympatrically at the type locality and has a similar chromosome number but a different karyotype with two very large chromosomes. darius Eckweiler & ten Hagen, 1998 Genetically this taxon from Central Elburs Mts. is most closely related to the allopatric A. posthumus from eastern Elburs. birunii Eckweiler & ten Hagen, 1998 This taxon was described as a subspecies of A. posthumus from central Elburs Mts. (Iran) but according to the genetic analysis, which agrees with the karyological results, this taxon must be considered specifically distinct from A. posthumus. caeruleus (Staudinger, 1871) This is the only Agrodiaetus species in this study which has blue-coloured females. It is distributed from Transcaucasus to Transcaspia and similar taxa also occur in Central Asia. In Elburs Mts. it occurs sympatrically with several taxa in this group (e.g. A. phyllis, A. posthumus, A. erschoffii and A. glaucias) which appear as the closest relatives in this study. erschoffii (Lederer, 1869) This species from Northeast Iran appears to be genetically most distinct from the other species in this group and its closest relatives remain unclear. carmon-group The ancestor species of this heterogenous group which is distributed from Anatolia to Iran appears to be A. iphidamon. carmon (Herrich-Schäffer, [1851]) This Anatolian taxon is the first in a group of four closely related and apparently allopatric taxa, some of which have a different karyotype. Specimens from very distant locations (Antalya Prov. in SW Turkey and Kars Prov. in NE Turkey) appear to be very similar genetically. 100 Systematics of Agrodiaetus schuriani (Rose, 1978) Although the sampled specimen of this taxon from Cappadokia is from a location which is between the two sampled localities of carmon, the sequences are quite distinct. This result indicates that the taxon schuriani is not a synonym of carmon, but further investigations are necessary to verify its status and distribution. A close relation to A. surakovi as indicated by ROSE (2002) seems possible on the basis of the molecular results. surakovi Dantchenko & Lukhtanov, 1994 This taxon represents A. carmon in Armenia and Azarbaijan but has a different karyotype. Genetically it is very similar to A. carmon. sekercioglui (Lukhtanov & Dantchenko, 2002) The COI data confirm that this taxon from SE Turkey is closely related to A. surakovi and was correctly placed as a subspecies of the latter. pierceae (Lukhtanov & Dantchenko, 2002) This taxon is very similar in phenotype to A. huberti (Carbonell, 1993) but has a different karyotype and seems to replace it in Southeast Turkey (Van and Hakkari Prov.). According to LUKHTANOV & DANTCHENKO (2002b) it is genetically (mtDNA) close to A. kendevani (Forster, 1956) and A. zarathustra neglecta (Dantchenko, 2000)2 and not to A. huberti despite the very similar appearance. The author can confirm the genetic distance to A. huberti. According to the COI data set A. pierceae is very close to A. schuriani but according to the ITS2 data set its relationships within the carmon-group are less clear because it appears to be quite distinct to any other members of this group. A. pierceae occurs sympatrically with A. surakovi sekercioglui at Çatak. The following 8 taxa represent a group of closely related taxa, some of which are allopatric to each other and may differ only karyologically. Their relationships are not well understood. Most of its members were included in the transcaspicus-group sensu HESSELBARTH et al. (1995). ninae (Forster, 1956) The first member of this group, which was described as a subspecies of A. transcaspicus but differs from it in karyotype, is distributed from Armenia to Eastern Anatolia turcicola (Koçak, 1977) This taxon which appears to replace A. ninae in Van Province (Turkey) can only be distinguished from the latter by its karyotype and the genetic data confirm that this taxon is very closely related to it. huberti (Carbonell, 1993) Prior to CARBONELL (1993) this taxon which is widely distributed in Northeastern Anatolia and occurs sympatrically with A. ninae was confused with the latter due to its similar appearance and identical karyotype. The nuclear and mitochondrial DNA data sets differ in the position of this taxon. A. huberti shares very similar COI haplotypes with A. ninae, but the ITS-2 sequences are distinct, confirming its specific status. elbursicus (Forster, 1956) Nominotypical material of this taxon from Elburs Mts. (Iran) appears to be very close to A. ninae according to the nuclear DNA data set, but quite distinct from it according to the mtDNA data set. The number of chromosomes is half the number found in A. ninae. zapvadi (Carbonell, 1993) The phenotype and karyotype of this taxon from Van Province (Turkey) is very similar to elbursicus, but genetically it is distinct. According to the mtDNA data set it is most closely related to A. huberti. zarathustra Eckweiler, 1997 This Iranian taxon from Lorestan has a peculiar phenotype and its karyotype is also different from the other members of the ninae-species group. Genetically it is very close to A. elbursicus (mtDNA) and A. zapvadi (ITS-2). 2 This taxon was described from Armenia but the name A. zarathustra neglecta Dantchenko, 2002 appears to be a nomen nudum because it was published without description and without stating any differentiating characters. 101 Chapter 4 arasbarani (Carbonell & Naderi, 2000) This taxon from Azarbaijan-e Sharqi (Iran) is similar to A. zarathustra in phenotype. Genetically it is closely related to A. elbursicus. paulae Wiemers & De Prins, 2003 The phenotype of this new species from Ahar Pass (Iran) is very distinct from any other member of this group and it has previously been confused with A. altivagans (ECKWEILER, pers. comm.). According to the genetic data it is most closely related to A. zarathustra, A. arasbarani, A. elbursicus and A. zapvadi and its karyotype is like those of the latter two taxa. dama (Staudinger, 1892) This very rare Anatolian species is genetically very distant from the other taxa in this group. It appears basal in the ITS-2 network and therefore might represent an ancestral taxon. hamadanensis (de Lesse, 1959) This Iranian species with a peculiar phenotype (males with violett wing upperside) is genetically very distant from other Agrodiaetus but seems to be most closely related to A. dama according to the mtDNA data set. theresiae Schurian, van Oorschot & van den Brink, 1992 The relationships of this local Anatolian endemic remain unclear although the mtDNA data set indicates A. surakovi as closest relative. Possibly this is another ancestral relict species comparable to A. dama. guezelmavi Olivier, Puplesiene, van der Poorten, De Prins & Wiemers, 1999 The genetic data confirm that this local Anatolian endemic is closely related to its sister species A. theresiae from which it differs only by its karyotype. antidolus (Rebel, 1901) This Eastern Anatolian species is the first in a group of 6 genetically closely related taxa. The first four taxa have males with a silvery-brown upperside and are allopatric in distribution. kurdistanicus (Forster, 1961) This taxon which is very similar to antidolus in phenotype but differs in chromosome number is only known from Van Province (Turkey). Genetically these karyotypically different specimens from Van Province do not differ from specimens with the antidolus-karyotype found in Hakkari Province. femininoides (Eckweiler, 1987) The darkest member of this group from Zanjan (NW Iran) again has a different chromosome number but genetically it is close to the former two taxa from Turkey. morgani (Le Cerf, 1909) The phenotype of this taxon from Iranian Kordestan and Lorestan is similar to A. antidolus but its chromosome number is the same as in A. femininoides. Its mtDNA is also similar to the latter. peilei Bethune-Baker, 1921 This rare species whose males have a unique golden brown wing colour occurs sympatrically with the former and the following species. It differs from them in karyotype and has a chromosome number intermediate between these two species. Genetically it is closely related to both of them and its COI haplotype is even identical with A. morgani. karindus (Riley, 1921) This Iranian taxon which occurs sympatrically with the former two species and differs from them in phenotype and karyotype appears to be misplaced in this group at first glance. Unlike the other members of the antidolus-species group its males display a bright blue upperside wing colour, similar to A. dama and therefore it was described as a subspecies of the latter. However the analysis of its mtDNA reveals that its haplotype is almost identical to A. peilei. Striking similarities to this species can also be found in the form of the wings and in the underside wing pattern. 102 Systematics of Agrodiaetus poseidon-group This group comprises mainly Anatolian, Caucasian, and few Iranian elements. Its ancestor species is probably A. iphidamon. hopfferi (Herrich-Schäffer, [1851]) This Anatolian species with conspicuous male phenotype (greyish blue upperside) appears to be the stem species of this group. poseidon (Herrich-Schäffer, [1851]) The genetic data confirm that this Anatolian species is closely related to A. hopfferi. It has a similar phenotype but blue-coloured males and a different karyotype. Its distribution closely resembles that of A. hopfferi but it is absent from Southeast Turkey. In Northeast Turkey it seems to be replaced by the following karyospecies: putnami (Lukhtanov & Dantchenko, 2002) Genetically this taxon from NE Turkey which was separated from A. poseidon because of its higher number of chromosomes seems to be very close to A. poseidon and its COI haplotype is almost identical. In the ITS-2 network however it clusters with the erschoffii-group, but this is due to only two plesiomorphic nucleotide character states shared with A. birunii but which are also found in A. iphidamon. Possibly this taxon is ancestral to A. poseidon. lycius (Carbonell, 1996) The genetic data confirm the view of ECKWEILER & HÄUSER (1997) that this local taxon which occurs near Antalya just off the Southwestern distributional limit of A. poseidon and A. hopfferi and which shares the karyotype of the former is closely related to both species. The following 8 taxa belong to a group of genetically very closely related taxa and most of them share very similar phenotypes. The reason why the MP- and Bayesian analysis produced unresolved trees is the fact that several populations appear as stem populations of others which can be seen in the network analysis (Fig. 82). This is usually the case in populations of the same species. Although karyological results indicate that several species are involved, there is no indication of genetic differentiation of such karyospecies. actis (Herrich-Schäffer, [1851]) This taxon from Central Anatolia is the first named of the actis-species group and apparently has a low chromosome number (n=17) although topotypical material has not been investigated yet. Genetically however it is not differentiated from the following taxa. firdussii (Forster, 1956) This taxon was described from Elburs Mts. and with n=31-34 its chromosome number seems to be about double the number in actis. Populations from Northwest Iran and Eastern Anatolia which were included in this study have a slightly lower and variable number (see chapter 2) but there is no indication of genetic differentiation between populations or specimens with lower (n=ca.25) and higher (n=28-30) number of chromosomes. pseudactis (Forster, 1960) With n=29 the chromosome number of this taxon from Azarbaijan appears to be the same as in many populations from Iranian Azarbaijan and eastern Anatolia. If populations of A. firdussii from Elburs Mts. should turn out to be specifically distinct, the name pseudactis might apply to populations from Northwest Iran and eastern Anatolia, otherwise the name might be sunk into synonymy with firdusii (as done by HESSELBARTH et al. 1995). A specimen from Armenia which was included in this study appears to represent the stem populations for all the others. This would indicate that the actis-species group originated in Armenia. ernesti Eckweiler, 1989 This local taxon which was described as a subspecies of A. firdussii from mountains near Antalya at the southwestern range limit of this species group is genetically more differentiated than any other taxon in this group. It also differs in phenotype (e.g. lighter blue upperside of the males). The chromosome number (n=18) is very similar to A. actis. HESSELBARTH et al. (1995) synonymized this taxon under A. sertavulensis, although both taxa have a different phenotype. This action is not supported by genetic and karyological data. 103 Chapter 4 artvinensis (Carbonell, 1997) Some populations from Northeast Turkey differ slightly in phenotype and chromosome number (n=21-22). Genetically however, these populations appear almost identical to those from Armenia (pseudactis). sigberti Olivier, van der Poorten, Puplesiene & De Prins, 2000 Some populations from the Taurus Mts. and the Pontic chain with dark phenotype are covered under this name. However, the phenotype of this taxon is very variable and many specimens cannot be separated from typical actis/firdussii/pseudactis. The karyotype is the same as in pseudactis. Genetically these populations do not seem to be differentiated either. Two specimens from the same population appear at slightly different places in the network. Although these specimens exhibit the extremes in phenotype variation found in this taxon, the genetic analysis also does not indicate that two different species are involved. haigi (Lukhtanov & Dantchenko, 2002) Populations from Southeast Turkey appear to have a slightly lower chromosome number (n=21-25) than surrounding populations from Northwest Iran and Northeast Turkey and such specimens were also included in the genetic analysis but they do not appear to be well differentiated genetically. The same probably applies to A. bilgini (Dantchenko & Lukhtanov, 2002) which was described from Torul (Prov. Gümüşhane) and has the same chromosome number as A. haigi. The remaining taxa form the crown group in Agrodiaetus and appear very closely related genetically. Due to the very similar phenotypes and karyotypes their systematics remains poorly understood. It appears that they are very young evolutionary units in the process of speciation. altivagans (Forster, 1956) The genetic analysis reveals that the material referred to this taxon in this study is heterogenous and might constitute several species, despite similar chromosome numbers (n=21-23). Alternatively it is one genetic unit with a variable phenotype and comprising several different haplotypes. In this case the following four taxa might represent karyologically diverged populations at the periphery of its range which are in the process of speciation and therefore not well differentiated genetically. The specimen from Armenia, where the type locality of A. altivagans is located, seems to occupy an ancestral position in the COI network analysis (Fig. 82) and it appears to be genetically distinct from the Turkish specimens: MW99165 from Erzincan (Erzurum Prov.) and MW99240 from Güzeldere Geçidi (Van Prov.) appear closely related to A. wagneri and A. maraschi whereas MW99353 and MW99357 from Güzeldere Geçidi (Van Prov.) are genetically similar to A. gorbunovi. The phenotype of the latter two specimens differs from typical altivagans and appears more similar to A. wagneri sensu HESSELBARTH et al. (1995) but this taxon has a different karyotype. wagneri (Forster, 1956) This taxon was treated as a subspecies of A. altivagans by DE LESSE (1962a) but elevated to species rank by HESSELBARTH et al. (1995) who claim that both taxa occur sympatrically in eastern Turkey. Their delimitation of this taxon however remains far from clear and the only karyological data available are from DE LESSE (1962a) who checked one specimen from the type locality which had n=16 chromosomes. The specimen included in this study is from Nevşehir which is 300 km east of the type locality in the centre of distribution of wagneri and 400 km west of the closest population of A. altivagans in Erzincan (according to HESSELBARTH et al. 1995). The phenotype of this specimen is typical for A. wagneri and several similar specimens from the same population are figured in HESSELBARTH et al. (1995: Plate 122, fig. 14-17). Its chromosome number is n=18 which is the same as recorded by De Lesse (1962a) from A. altivagans of Erzincan Prov. Genetically this specimen is also very similar to A. altivagans from Erzincan (MW99165). Two further specimens which are similar to A. wagneri sensu HESSELBARTH et al. (1995) from Van Province (MW99353 and MW99357) turned out to have the same chromosome number as A. altivagans (n=21-23) and are therefore placed under this taxon. Genetically these two specimens do not appear closely related to A. wagneri. 104 Systematics of Agrodiaetus maraschi (Forster, 1956) HESSELBARTH et al. (1995) synonymized this taxon from Maraş under A. wagneri, partly because of its identical chromosome number (n=16) which DE LESSE (1962a) reported from Kayseri, 170 km west of its type locality. The genetic analysis includes a specimen from Gürün (Sivas Prov.), 130 km north of the type locality whose phenotype appears to be very similar to the holotype of A. maraschi. Its chromosome number also turned out to be n=16. Genetically it is almost identical to A. altivagans from Erzincan and Van Prov. (specimen MW99240, n=21). sertavulensis (Koçak, 1979) HESSELBARTH et al. (1995) raised this taxon from the Taurus Mts. in Turkey to species level and synonymized A. ernesti with it, one reason for this action being the alleged sympatry of A. sertavulensis with A. wagneri at the type locality. Genetically however this taxon appears to be almost identical to A. wagneri and A. maraschi but distinct from A. ernesti. A. sertavulensis (n=20) seems to represent an allopatric population of the altivagans-complex and the “sympatric” wagneri might be just an intrapopulational variation. gorbunovi Dantchenko & Lukhtanov, 1994 This is another allopatric taxon of the altivagans-complex from Azarbaijan. Genetically specimens from three different populations in Northwest Iran are almost identical with each other and with two A. altivagans from Van Province (Turkey). damocles (Herrich-Schäffer, [1844]) This Russian taxon with a chromosome number of n=24-27 has only recently been recorded from Turkey (Erzincan Prov.) and described as ssp. kanduli (LUKHTANOV & DANTCHENKO, 2002). The holotype is phenotypically very similar to A. wagneri sensu HESSELBARTH et al. (1995). According to the mtDNA data set of LUKHTANOV & DANTCHENKO (2002) this taxon is closely related to A. damocles damocles, A. damocles rossicus, A. damocles krymaeus and A. altivagans. In this study a specimen from Çatak (Van Province) is included which resembles A. damocles kanduli in phenotype and which has the same chromosome number (n=25). Genetically it turned out to be very similar to A. gorbunovi and to A. altivagans from Güzeldere Geçidi (Van Province) with n=22. It seems that karyological results do not coincide well with phenotypic or genetic variation in this complex and further karyological investigations are necessary to clarify species boundaries. At present, the genetic data only indicate the existence of one species (A. damocles) with a variable phenotype and karyotype which comprises the taxa damocles, altivagans, wagneri, maraschi, sertavulensis & gorbunovi. mofidii (de Lesse, 1963) This Northeast Iranian taxon from Kopet Dagh (ssp. mofidii, n=34-35) and Kuh-e-Sorkh (ssp. sorkhensis Eckweiler, 2003, n=45) is genetically very close to A. altivagans to which it is allopatric in distribution. mithridates (Staudinger, 1878) The systematic position of this monomorph Anatolian species with a variable chromosome number of n=21-27 has been uncertain. It was placed into the admetus-group (with monomorph males) by BÁLINT & JOHNSON (1997), but into the dolus-group (with silvery males) by HESSELBARTH et al. (1995) and ECKWEILER & HÄUSER (1997). Genetically it is closely related to A. sennanensis and A. hopfferi according to the nuclear DNA data set, but to A. altivagans and A. maraschi according to the mtDNA data set. The range of A. mithridates appears to be the intersection of the range of A. hopfferi with the range of the A. altivaganscomplex. sennanensis (de Lesse, 1959) This northwest Iranian taxon appears to be closely related to the allopatric A. hopfferi (ITS-2) and to the sympatric A. cyaneus (COI). pseudoxerxes (Forster, 1956) The closest relative of this Iranian taxon from Elburs Mts. seems to be A. gorbunovi and A. cyaneus. ECKWEILER & HÄUSER (1997) list it as a subspecies of A. kendevani although De Lesse (1962a) found both taxa sympatrically at Kendevan Pass and treats A. pseudoxerxes as a subspecies of A. altivagans and the close relationship with this species can now be confirmed 105 Chapter 4 genetically. According to the mtDNA data set of Lukhtanov & Dantchenko (2002) A. kendevani is genetically closely related to A. pierceae and A. zarathustra both of which belong to the carmon-group. This result also rules out conspecifity of A. pseudoxerxes with A. kendevani. cyaneus (Staudinger, 1899) This taxon was described from Georgia and is distributed from the Caucasus and Kordestan to Iran. Genetically it appears distinct but closely related to the altivagans-complex. Specimens from Van Province (Turkey) and Marand (Iranian Azarbaijan) which belong to the nominate subspecies are very similar genetically, but the specimen of ssp. damalis (Riley, 1921) from Lorestan in Iran appears to be genetically distinct. merhaba De Prins, van der Poorten, Borie, Oorschot, Riemis & Coenen, 1991 This taxon is similar to A. cyaneus in phenotype and karyotype and only known from northeastern Anatolia (Artvin and Erzurum Prov.). The genetic data confirm its close relationship with A. cyaneus to which it is allopatric in distribution and it might be more appropriate to treat it as a subspecies of A. cyaneus. 106 Evolution of morphological traits in Agrodiaetus Chapter 5: Evolution of morphological traits in Agrodiaetus Introduction Although differences in wing pattern and coloration between many Agrodiaetus species are very subtle to anyone who is not familiar with this group of butterflies, they often represent the only means of identification and classification. Yet even experts are often unable to determine single specimens because of considerable infraspecific variation (within and between populations). On the other hand, the subgenus Agrodiaetus includes also very different colour morphs. Even though the majority of species has males with an iridescent blue upperside, like the majority of Lycaenidae, the subgenus Agrodiaetus also includes many species with different upperside coloration, most of them brown, some silvery, whitish or even golden brown. Together with the extent of androconial patches, these colour morphs have even been the main characters to delimit species groups within Agrodiaetus. The iridescent coloration is confined to the males, whereas females of most Agrodiaetus species are dull brown. It therefore appears that the male coloration has evolved through sexual selection. Females of the closely related Polyommatus icarus only mate once and exhibit mate choice (KNÜTTEL & FIEDLER 2001), and it should be noted that butterflies discern colour very differently from the human eye, because they also have colour receptors for the ultraviolet light spectrum. Unfortunately no studies have been conducted to infer the coloration of Agrodiaetus males as it would appear to the eye of the butterfly, and not even the visible colour has been measured and analysed quantitatively. A quantitative analysis of wing pattern characters by means of multivariate techniques is also beyond the scope of this thesis (but hopefully can be conducted in the future). This chapter aims to pinpoint four important morphological characters which have been used for the systematics of Agrodiaetus and evaluate them in a qualitative approach: 1. Presence of a distinct white streak on the hindwing underside 2. Presence of orange submarginal lunules on the hindwing underside 3. Presence of well developed androconial patches on the forewing upperside of males 4. Ground colour of the male upperside The first character is the main character used to delimit the subgenus Agrodiaetus although some species or individuals with the white streak missing are still regarded as members of this subgenus due to other distinctive characters. The remaining characters have been used in combination to characterize species groups within Agrodiaetus. Material and methods The presence of the first three characters (white streak, orange submarginal lunules and androconial patches) was checked from the wing vouchers. 1211 wing vouchers of Agrodiaetus and 521 wing vouchers of outgroup species which are kept in glass slide mounts were available for the analysis (Appendix 2). Wings were also scanned with a Microtek Scanmaker E6 at 600 dpi resolution in RGB Modus (16.7 million colours) and will be made available online through MorphBank (http://www.morphbank.net/). The ground colour of the forewing upperside was copied from a representative spot in the area of the cell using Adobe Photoshop and pasted onto the nodes of the ITS2-network for visualization. 107 Chapter 5 Results A distinct white streak was found in most Agrodiaetus but not in any outgroup species. Within Agrodiaetus the white streak was found missing in the following species or specimens: Clade admetus-clade admetus-clade dolus-clade dolus-clade dolus-clade carmon-clade carmon-clade carmon-clade carmon-clade carmon-clade carmon-clade carmon-clade erschoffii-clade poseidon-clade poseidon-clade Species Agrodiaetus admetus Agrodiaetus lorestanus Agrodiaetus fabressei Agrodiaetus humedasae Agrodiaetus aroaniensis Agrodiaetus hamadanensis Agrodiaetus dama Agrodiaetus karindus Agrodiaetus peilei Agrodiaetus femininoides Agrodiaetus morgani Agrodiaetus antidolus Agrodiaetus tenhageni Agrodiaetus sennanensis Agrodiaetus mithridates Specimens with missing streak all males, females streak reduced WE02535 (=1/2) all all JC00040, 041, 047 (=3/4) all all all all all all MW99376-379, 393, 404 (=7/11) all all very weak (1) Orange submarginal lunules were present in the following species: Clade iphigenides-clade poseidonides-clade poseidonides-clade Species Agrodiaetus iphigenides Agrodiaetus poseidonides Agrodiaetus dagmara Androconia are present in all Agrodiaetus but in some species they are organized in hairy androconial patches on the forewing upperside. The extension of these patches however is subject to considerable variation and is difficult to measure. The following list includes only species with extensive androconial patches which are not restricted to the wing venation: Clade admetus-clade dolus-clade carmon-clade carmon-clade carmon-clade carmon-clade carmon-clade poseidon-clade poseidon-clade poseidon-clade Species all all Agrodiaetus peilei Agrodiaetus femininoides Agrodiaetus morgani Agrodiaetus kurdistanicus Agrodiaetus antidolus Agrodiaetus hopfferi Agrodiaetus sennanensis Agrodiaetus mithridates Specimens all partial all all all partial all all The ground colour of the reference specimens in the ITS2-network (see Fig. 84) is presented in Fig. 86 and the presence of well-developed androconial patches is indicated with an ‘A’. The species names of the reference specimens can be found in Fig. 84 and Tab. 11. 108 Evolution of morphological traits in Agrodiaetus Fig. 86. Ground colour and presence of androconia (A) mapped on ITS2-MP-network 109 Chapter 5 Discussion HÄUSER & ECKWEILER (1997) state the presence of a distinct white streak on the hindwing underside which is „sometimes entirely absent but never partly present (as in other groups of Polyommatus)” as the only morphological diagnostic feature for the delimitation of the subgenus Agrodiaetus. (The second diagnostic feature stated is the oligophagy on Onobrychis and Hedysarum.) The molecular results have confirmed the delimitation of Agrodiaetus sensu HÄUSER & ECKWEILER (1997) as a monophyletic group within Polyommatus and also the exclusion of Polyommatus thersites which has a vestigial white streak (like many Polyommatus species) and whose larva also feeds on Onobrychis. (P. thersites is included within Agrodiaetus e.g. by BÁLINT & JOHNSON (1997) and TOLMAN & LEWINGTON (1997).) It can also be confirmed that the loss of the white streak in several Agrodiaetus species occurred independently in different clades and that there is individual variation of this character in some species. According to HESSELBARTH et al. (1995) the streak is mostly absent in A. admetus but can sometimes be present and in females it is often only partly present (contrary to the statement in HÄUSER & ECKWEILER 1997). In A. mithridates the variation of this character is considerable with the white streak present, hardly visible or totally absent. A. demavendi lorestanus Eckweiler, 1997 was described because of the absence of the white streak in 90% of the individuals. HESSELBARTH et al. (1995) discuss the possibility that the white streak represents a diagnostic character to separate the two karyospecies A. antidolus and A. kurdistanicus. In the material used for this thesis all specimens of the karyospecies A. kurdistanicus (from Van Province) had a white streak. In A. antidolus (from Hakkari Province) the white streak was absent in all specimens from Yüksekova and Dilezi Geçidi but present in a part of the specimens from Haruna Geçidi and Dez valley. Specimens of A. antidolus with and without white streak were included in the molecular study which did not reveal any genetic differentiation between these two forms. In summary, there is good evidence to accept the white streak as an autapomorphic character state of Agrodiaetus, which has subsequently been lost multiple times, most notably in the antidolus subclade. Orange submarginal lunules were only present in the three Central Asian species. (This character can be found in many further Central Asian Agrodiaetus species which could not be included in this study.) The presence of such lunules is a plesiomorphic character which is found in most other species of the genus Polyommatus (but not in Meleageria s.str. and Neolysandra). Therefore these Central Asian species might be thought to represent ancestral taxa but this does not appear to be true from the molecular analysis. However, this possibility can not be entirely ruled out, because Polyommatus species confined to Central Asia were not sampled and the sister group to Agrodiaetus might be found there. According to ECKWEILER & HÄUSER (1997) the upperside coloration and presence of welldeveloped androconial patches are the main characters to delimit species groups within Agrodiaetus. Apart from the Central Asian taxa which were separated due to their biogeographical origin (iphigenides- and dagmara-group), despite of differences in upperside coloration and androconial patches, all other blue-coloured Agrodiaetus with sparsely developed androconial patches were grouped together (damon-group) and the taxa with well developed androconial patches were split into three groups, one with only brown-coloured males (admetus-group), one with only blue-coloured males (dama-group) and one with differently (including white-) coloured males (dolus-group). From Fig. 86 it appears that well-developed androconial patches were only found in non-blue-coloured males, a discrepancy to ECKWEILER & HÄUSER (1997) which needs explanation. Some taxa of their dama-group (like A. theresiae) have slightly better developed androconia than other blue Agrodiaetus but these are not comparable to those found in the admetus- and dolus-group. In A. hamadanensis the androconial patches are hardly developed at all. Individual variation can 110 Evolution of morphological traits in Agrodiaetus also explain some differences in judgement between authors. The most extreme variation was observed in A. hopfferi, a species with variable bluish-silvery coloured males which was placed in the damon-group by ECKWEILER & HÄUSER (1997). Some specimens have welldeveloped androconial patches whereas these are hardly visible in others and absent in the more bluish males from Hakkari Province. On the other hand, two specimens with brown males appear in Fig. 86 which do not have well-developed androconial patches. One of them is A. glaucias, a member of the erschoffii-group, which still has blue basal suffusion on the wing upperside. The same is true for specimen WE02671 of A. femininoides from Ardabil Province whereas A. femininoides specimens from Zanjan Province, which have only slight traces of basal suffusion, all have well-developed androconial patches. To conclude, it appears that androconial patches are especially well developed in species with non-blue-coloured males. This makes sense because a well-developed scent-based mate recognition system is needed in monomorph Agrodiaetus to replace the visual mate recognition system. A second question concerns the systematic value of these characters. Fig. 86 reveals that the admetus-clade only consists of monomorphic brown species and the dolus-clade only contains species with brown- or white-coloured males, but A. sennanensis and A. mithridates are found within the poseidon- and several other brown Agrodiaetus in the carmon-clade. In the case of A. mithridates it was noted already by HESSELBARTH et al. (1995) that this species can not be closely related to the monomorphic Agrodiaetus of the admetus-group, e.g. because of the different structure of the androconial patches. Differences can also be found in other taxa, e.g. in the antidolus-species group where the androconial patch is limited to the area below the central cell whereas it extends along the costal vein in the admetus- and dolus-clade. A most interesting case consists of the three taxa A. peilei (brown males), A. morgani (silvery males) and A. karindus (blue males) which seem to be very closely related (with almost identical nuclear and mtDNA haplotypes) and occur sympatrically in Lorestan (Iran), but have different karyotypes. Although the underside wing pattern is extremely similar in these three taxa they were even placed into different groups due to the striking differences in upperside wing colour. This case might be an interesting example of a sexually selected character displacement reinforcing premating isolation. Current data suggest that in Agrodiaetus the loss of the blue iridescent wing colour coupled with the expansion of the androconial patches requires few genetic changes and happened independently several times in the course of the radiation of Agrodiaetus. Therefore the value of these characters to infer the systematics and evolutionary history of Agrodiaetus is limited, and the group of “brown” Agrodiaetus apparently is no monophyletic unit. Discoloration, the loss of the iridescent blue colour is also found in many other genera of Lycaenidae, see BÁLINT & JOHNSON (1997) for an overview, but most species are found within Agrodiaetus and Aricia. The reasons for it are unknown in most cases, but in Meleageria (daphnis) marcida discoloration appears to be an adaptation to local climatic conditions of the northern Elburs with reduced solar radiation (BIRÓ et al. 2003). This could also explain the brown colour of Agrodiaetus valiabadi which occurs in the same area, but it is less plausible for most other brown Agrodiaetus species. Instead, sexual selection might be the driving force in these cases. Further studies coupled with experiments and wing colour measurements which must include the hidden wing pattern in the UV spectrum (like KNÜTTEL & FIEDLER 2001 in Polyommatus icarus) could reveal exciting insights into the evolution of mate recognition systems in Agrodiaetus. 111 Chapter 5 Summary A qualitative analysis of major wing pattern characters in Agrodiaetus revealed that the distinct white streak appears to be an autapomorphy of Agrodiaetus but secondarily can get lost again as an individual variation. The presence of a plesiomorphic wing character in Central Asian Agrodiaetus would suggest an ancestral position but this is not corroborated by the molecular analysis. An obvious strong correlation exists between the loss of blue iridescent coloration of males and an enlargement of their andronconial patches which indicates a switch from a visual sexual recognition system to an olfactorial one. Both character states evolved independently several times in Agrodiaetus and can not be used to define species groups within Agrodiaetus. 112 The role of chromosome evolution in the radiation of Agrodiaetus Chapter 6: The role of chromosome evolution in the radiation of Agrodiaetus Introduction Although the incredible variation in chromosome numbers among Agrodiaetus is well-known since the pioneering work of DE LESSE (1960a), the reasons for this pattern are not understood. HESSELBARTH et al. (1995) hypothesize that fission of chromosomes should increase the ability of species to adapt to new environmental conditions, because exchange of chromosomal material during meiosis would be facilitated. Correspondingly, chromosome fusions should lower the recombination potential leading to a more stable genome which would be beneficial for species already adapted to specific, more stable environmental conditions. However, these predictions have never been empirically tested. If these predictions are true, a positive correlation between the number of chromosomes and range size should be expected, because species with very restricted ranges are usually specifically adapted to local conditions, whereas widely distributed species accept broader amplitudes of habitat conditions. This hypothesis will be tested in this chapter. Another question concerns the role of karyotype evolution in the radiation of Agrodiaetus. Is it the cause for the high number of species in this subgenus? Did it even cause sympatric speciation? In Metazoa sympatric speciation has hardly been proven, but convincing cases are found among recent radiations of freshwater fishes (e.g. MCCUNE & LOVEJOY 1998; RUNDLE et al. 2000; SCHLIEWEN et al. 2001) and it is common in plants where polyploidy often leads to speciation. Putting karyological results into a phylogenetic framework should help to elucidate these questions. Other interesting aspects of karyotype evolution include biogeographical patterns and phylogenetic signal in chromosome numbers. Do species with high or low chromosome numbers occur in certain biogeographical areas? Is there a direction of change in chromosome numbers? Do numbers change gradually or by stepwise multiplication or division? This chapter aims to find answers to these questions. Material and methods Chromosome numbers (or their mean in case of a range of numbers) were plotted against the range size of all currently known karyospecies (N=88). A rough score of the range size (RS) was assigned in analogy to KUDRNA (1986), but adapted for the Palaearctic region: 1. Species widespread over the whole (or nearly all of) the Palaearctic region; 2. Species widespread over large parts of the Palaearctic region; 3. Species distributed over one or more smaller parts of the Palaearctic region; 4. Species restricted to one or more territories smaller than the above; 5. Species confined to a small area, such as one mountain range, or a single (known) site. The Spearman rank-difference correlation coefficient was calculated to test for any (positive or negative) correlation between both variables and a Kruskal-Wallis-ANOVA was also conducted to check correlations between range size and chromosome numbers. These tests appear justified because no indication exists that range size is depending on the phylogenetic position. Two methods were applied to investigate the karyological data in a phylogenetic framework. Firstly, statistical parameters were calculated for each clade obtained from the combined COIand ITS-2 phylogenetic analysis in order to see if patterns of chromosome evolution differ 113 Chapter 6 between clades. All clades with only one or two taxa were lumped together. The chromosome numbers used were the mean values for each recognized taxon listed in chapter 2 taking into account own as well as literature data. In a second approach, chromosome numbers were mapped onto the ITS-2 phylogenetic network to look for evidence of any direction in karyotype evolution. In this case actual chromosome numbers of the specimens used to construct the network were applied. Only if such data were not available, chromosome numbers were inferred from other sources (including literature data) and marked as such. If chromosome numbers change gradually and a direction of chromosome evolution exists, taxa which are distant from the supposed ancestral haplotype of A. damon should have a more derived karyotype (i.e. highly fissioned or highly fused) than those near the origin of the network. Results A list of all recognized karyospecies in alphabetical order together with their (mean) haploid chromosome number (n) and range size (RS) is found in Tab. 14 and the number of taxa attributed to each range size category are shown in the following table: RS Taxa 1 0 2 3 4 5 2 14 39 33 The correlation between both variables is shown in Fig. 87. Tab. 14. List of species with (mean) haploid chromosome number and range size n RS Taxon n RS Taxon n RS Taxon n RS Taxon 17 4 dantchenkoi 41 5 iphicarmon 29 5 poseidon 21 4 actis 79 3 deebi 67 5 iphidamon 14 4 poseidonides 24 4 admetus 109 5 demavendi 71 3 iphigenia 14 3 posthumus 85 5 ainsae 20 3 dizinensis 17 5 iphigenides 66 3 pseudactis 29 4 alcestis 21 4 dolus 124 5 juldusus 67 4 pseudoxerxes 16 4 altivagans 41 4 ectabanensis 18 4 karindus 67 4 putnami 26 5 antidolus 114 4 elbursicus 17 4 khorasanensis 84 4 ripartii 90 2 ardschira 48 5 eriwanensis 32 4 klausschuriani 56 5 sennanensis 29 4 aroaniensis 22 5 ernesti 18 5 kurdistanicus 60 5 sertavulensis 20 5 artvinensis 4 erschoffii 14 3 lycius 22 5 shahrami 130 5 aserbeidschanus 23 27 4 fabressei 90 4 maraschi 16 5 shamil 17 4 baytopi 11 5 femininoides 27 4 menalcas 85 3 sigberti 27 4 birunii 20 4 firdussii 28 3 merhaba 17 5 surakovi 50 4 caeruleus 82 3 fulgens 103 5 mithridates 24 3 tankeri 21 5 carmon 16 4 galloi 66 5 mofidii 35 4 theresiae 63 5 carmonides 16 4 gorbunovi 20 4 morgani 26 4 transcaspicus 53 4 ciscaucasicus 18 4 guezelmavi 42 5 ninae 35 4 turcicola 20 5 cyaneus 40 4 hamadanensis 22 4 paulae 17 5 turcicus 24 4 dagestanicus 41 5 hopfferi 15 3 peilei 39 5 valiabadi 23 5 dama 25 3 huberti 35 4 pfeifferi 107 4 wagneri 17 4 damocles 45 2 humedasae 38 5 phyllis 80 3 zapvadi 19 5 damon 67 3 interjectus 31 5 pierceae 22 5 zarathustra 22 4 damone 114 Chromosome num bers The role of chromosome evolution in the radiation of Agrodiaetus 140 120 100 80 60 40 20 0 2 3 4 5 Range size Fig. 87. Correlation between chromosome numbers and range Neither the Spearman Rank Correlation Coefficient (R = 0.053, p>= 0.621, Z = 0.494) nor the Kruskal-Wallis-ANOVA (H=3.74, p=0.2914) returned a significant result for a correlation between both variables. Fig. 88 displays the variation of chromosome numbers and Tab. 15 lists statistical parameters for each clade, apart from the few species of the ancestral damon-, iphidamon-, iphigenides- and poseidonides-clade which were lumped together (“others”). Tab. 15. Statistical parameters of chromosome number variation in Agrodiaetus clades admetus dolus iphigenia erschoffii carmon poseidon others Total 6 11 5 7 20 23 5 77 66 19 12 10 16 15 14 10 90 125 29 131 82 45 67 131 80.9 64.8 22.9 56.4 38.9 21.8 33.2 40.9 81.5 48.0 24.0 56.0 37.0 20.5 24.0 27.0 8.5 38.2 5.9 44.6 18.3 5.2 22.0 29.0 Number of taxa Minimum Maximum Mean Median Standard deviation 30 25 admetus-clade dolus-clade 20 Number of taxa 15 iphigenia-clade erschoffii-clade 10 5 carmon-clade poseidon-clade 10 20 30 40 50 60 70 80 90 100 110 120 130 0 To po ta l carmseido n-c e rs on-cl lad e iphig choff ii-clad e d olu en ia-cl ade adme s-clade ade tu s-c Clades lade Total Chromosome number (n) Fig. 88. Variation of chromosome numbers in different Agrodiaetus clades 115 Chapter 6 Different clades have significantly different chromosome numbers (Tab. 16). Tab. 16. Kruskal-Wallis-ANOVA: Chromosome number differences between clades. Chromosome no. by Clade admetus-group dolus-group iphigenia-group erschoffii-group carmon-group poseidon-group others Kruskal-Wallis statistic p n 4 10 5 7 20 21 5 23.33 0.0007 Rank sum 256.5 520.5 136.0 281.5 794.5 481.0 158.0 Mean rank 64.13 52.05 27.20 40.21 39.73 22.90 31.60 (chisqr approximation, corrected for ties) The ITS-2 MP-network with chromosome numbers mapped onto it is shown in Fig. 89. The taxa names can be looked up in Fig. 84. If chromosome numbers were inferred from other specimens than those used to construct the network, these are shown in brackets {}. Subnetworks which resemble the clades obtained in the phylogenetic trees are named and marked with a polygon, and exceptions are also labelled. All taxa in the admetus-group and most taxa in the dolus-group have high chromosome numbers whereas low chromosome numbers prevail in the remaining groups. Exceptions are A. posthumus (n=85) in the erschoffii- and A. carmon in the carmon-group (n=81-82). Low chromosome numbers are often found in stem species (e.g. A. birunii (n=10-11) in the erschoffii-group, A. iphigenia (n=13-14) in the iphigenia-clade, A. hopfferi (n=15-16) in the poseidon-group and A. alcestis (n=19-21) in the dolus-group whereas a higher chromosome number appears to be a derived condition. Numbers between n=30 and n=60 are common in the dolus- and especially the carmon-group but hardly found in most other groups. Exceptions are A. damon (n=45) and two taxa in the poseidon-group, A. mofidii sorkhensis, n=45 and A. klausschuriani (n=56), but the phylogenetic position of the latter taxon is uncertain. Discussion No significant correlation was found between chromosome numbers and range size which questions the assumption that the number of chromosomes influences the ability of a species to adapt to environmental conditions. Circumstantial evidence for differing habitat requirements between taxa with low and taxa with high chromosome numbers is not apparent either. Closely related karyospecies inhabit similar habitats, e.g. A. alcestis (n=19-21) and A. menalcas (n=85) in Anatolia, A. birunii (n=10-11) and A. posthumus (n=85) in central and eastern Elburs Mts. or A. morgani (n=27) and A. karindus (n=66-68) in Lorestan, Iran. Currently it seems more reasonable to assume that karyological differences are not adaptive, but occur more or less accidentally in Agrodiaetus. 116 The role of chromosome evolution in the radiation of Agrodiaetus Fig. 89. Chromosome numbers mapped onto the ITS2-MP-network 117 Chapter 6 In order to see if there is a directional change of chromosome numbers in the radiation of Agrodiaetus, we shall have a first look at possible sister species of the ancestral A. damon, which has a chromosome number of n=45. Such a number is not found in any close relative of Agrodiaetus (apart from Lysandra bellargus) and appears to be caused by fission of the modal and probably ancestral number of n=22-24 which is found in most Polyommatus species. Species with higher numbers are only found in the derived subgenus Lysandra (reaching from n=24 in the ancestral L. syriaca to n=87-92 in L. coridon) and even higher in a group of closely related species sometimes united into the subgenus Plebicula (LORKOVIĆ 1990): P. dorylas (n=147-151), P. nivescens from Spain (n=190-191) and P. atlanticus from Morocco (n=221-223). Of interest is also the variation found within P. cornelia (n=26-38) which appears closely related to A. damon in the ITS-2 network. The closest relatives of A. damon among Agrodiaetus according to the ITS-2 network have chromosome numbers which are multiples or fractions of n=45: almost triple in A. shahrami (n=130), double in A. ripartii (n=90), half in A. valiabadi (n=23) and a third in A. iphidamon (n=14). Thus it appears that considerable variations in chromosome numbers have appeared early in the radiation of Agrodiaetus. Accordingly, chromosome numbers also vary in each Agrodiaetus clade, although differences can be seen between clades. The admetus-clade has the highest mean number and variation is low. The highest variation is found in the (mainly Iranian) erschoffiiclade which also has the taxa with the lowest (A. birunii, n=10) and highest (A. shahrami, n=130) chromosome number found in Agrodiaetus. The poseidon-clade which includes mainly Anatolian faunal elements has the lowest variation and also the lowest mean in chromosome numbers, most of which vary between n=15 and n=35. Most chromosome number changes in this group (and part of the carmon-group) are due to slight variations of the modal value 24. On the other hand, high chromosome numbers seem to appear suddenly, probably by simultaneous fission of the whole chromosome set which leads to multiple numbers. Phylogenetically they appear to be in a deadlock position. Fig. 89 does not show any clear case where highly fissioned chromosomes were fused again to low numbers. Interestingly, the most extreme values are not found in the most derived taxa but they appear mainly in ancestral clades. Chromosome numbers in the poseidon-group, which turned out to be the crown group of Agrodiaetus in the molecular phylogenetic analysis and which includes many taxa in the process of speciation, has the lowest variation in chromosome numbers most of which are still around the modal value. This also indicates that most of the drastic changes of chromosome numbers happened early in the radiation of Agrodiaetus and therefore corroborates the taxonomic practice to attribute species status to karyospecies with highly divergent chromosome numbers. Most closely related karyospecies are allopatric or parapatric in distribution. If karyologically well-differentiated sister species occur in sympatry, they also differ considerably in phenotype: A. alcestis (brown males, n=19-21) and A. menalcas (white males, n=85) in Anatolia, A. hopfferi (silvery males, n=15) and A. poseidon (blue males, n=19-22) in Anatolia, or A. morgani (silvery males, n=27), A. peilei (brown males, n=39) and A. karindus (blue males, n=66-68) in Lorestan, Iran. Apparently the coexistence is only possible if females can differentiate the males of closely related species by their phenotype. Otherwise extensive hybridization between different “karyospecies” would occur and difficulties in the pairing of chromosomes during meiosis would lead to a very low fitness of the offspring. Therefore it can be concluded that there is little evidence for sympatric speciation caused by changes in karyotype. Instead, changes in chromosome number seem to be a common by-product of allopatric speciation and phenotypic differentiation is necessary for sister species to occur in sympatry. However, it is probable that chromosomal changes have been important for the 118 The role of chromosome evolution in the radiation of Agrodiaetus radiation of Agrodiaetus during the Pleistocene. Due to periodically changing climate conditions, altitudinal range shifts of populations may have lead to their isolation from each other during the interstadia. Chromosomal changes which occured in allopatric populations confined to isolated mountain tops did not allow remixing of gene pools when their ranges met again during recurrent periods of cooler climate. These processes could explain the high species diversity in Agrodiaetus, in particular in areas with complex topographic relief such as Anatolia, Transcaucasia or Iran. Summary A correlation analysis of chromosome numbers and range size did not support the hypothesis that the number of chromosomes has an adaptive value. A comparison of chromosome numbers between evolutionary units revealed different patterns. High numbers dominate in the two clades with exclusively brown or silvery-white males. Fission events have happened early in the radiation of Agrodiaetus and independently within different clades. A high degree of fragmentation appeared to represent an irreversible condition when chromosome numbers were mapped onto a phylogenetic network. No evidence was found that changes of chromosome numbers lead to sympatric speciation, rather karyotype evolution in allopatry could have prevented introgression between genotypes if allopatrically evolved taxa came secondarily into sympatry. In this regard, karyotype differentiation may have played a major role in the radiation of Agrodiaetus during the Pleistocene. 119 Summary Summary The subgenus Agrodiaetus which is distributed extensively in the Palaearctic region and especially in Southwest Asia is a species-rich group of blues which are often very similar morphologically but show a large variation in their chromosome numbers (n=10-125). The karyology of 64 taxa was successfully studied and the previously unknown chromosome numbers of 17 taxa were revealed. By means of molecular techniques it was possible to clarify the phylogeny of this group for the first time. A. damon, a species which is extremely widespread in the Palearctic region and has a constant chromosome number of n=45, is the sister-species of all other species of Agrodiaetus that have been tested. Presumably the colonization of Europe took place before the main radiations in the Anatolian-CaucasianIranian region. The greatest differences in the number of chromosomes were found in allopatrically distributed sister-species. It was surprising that morphologically extremely similar sympatric species with different numbers of chromosomes were not closely related, but often belonged to totally different clades, whereas some closely related sympatric species differed greatly in wing colours. This leads to the conclusion that changes in the number of chromosomes do not lead to sympatric speciation, but instead appear as a by-product of allopatric speciation and that such young species can only occur in sympatry after a sufficient differentiation in their phenotype to exclude erroneous matings. The comparison of mitochondrial with nuclear DNA sequences has also shown that hybridizations in Agrodiaetus are rare events. Supposedly they do not occur more frequently in Agrodiaetus than in other Lycaenidae. Only one of the specimen tested, which already drew our attention by its intermediate wing coloration, after molecular testing proved to be a hybrid of two nearly related but quite differently coloured species. Zusammenfassung Die in der Paläarktis und besonders in Vorderasien weit verbreitete Untergattung Agrodiaetus ist eine sehr artenreiche Gruppe von Bläulingen, welche sich morphologisch oft sehr ähnlich sehen, sich aber durch eine extrem starke Variation ihrer Chromosomenzahlen (n=10-125) auszeichnen. 64 Taxa konnten karyologisch erfolgreich untersucht und die bisher unbekannte Chromosomenzahl von 17 Taxa aufgedeckt werden. Mit Hilfe molekularer Techniken ist es nun erstmals gelungen, die Phylogenie dieser Gruppe weitgehend aufzuklären. Demnach ist A. damon, eine Art mit extrem weiter Verbreitung in der Paläarktis und konstanter Chromosomenzahl (n=45), die Schwesterart aller übrigen untersuchten Agrodiaetus-Arten. Die Besiedlung Europas erfolgte vermutlich schon vor den Hauptradiationen im anatolischkaukasich-iranischen Raum. Die größten Unterschiede in den Chromosomenzahlen finden sich bei allopatrisch verbreiteten Schwesterarten. Überraschend war, dass morphologisch extrem ähnliche sympatrisch vorkommende Arten mit unterschiedlichen Chromosomenzahlen nicht näher miteinander verwandt sind, sondern oft sogar zu ganz verschiedenen Verwandtschaftsgruppen gehören, wohingegen sich einige sehr nahe verwandte sympatrisch vorkommende Arten stark in der Flügelfärbung unterscheiden. Daraus lässt sich der Schluss ziehen, dass Veränderungen der Chromosomenzahlen nicht zu sympatrischer Artbildung führen, sondern stattdessen ein Nebenprodukt allopatrischer Artbildung darstellen und dass solche jungen Arten erst dann wieder in Sympatrie auftreten können, wenn sie sich auch phänotypisch ausreichend differenziert haben, um Fehlpaarungen zu vermeiden. Der Vergleich von mitochondrialen mit Kern-DNA-Sequenzen hat auch gezeigt, dass Hybridisationen bei Agrodiaetus seltene Ereignisse darstellen. Sie treten vermutlich nicht viel 120 Acknowledgements häufiger auf als bei anderen Lepidopteren-Gattungen. Nur einer der untersuchten Falter, der bereits durch eine intermediäre Färbung aufgefallen war, erwies sich nach molekularer Untersuchung als Hybrid zwischen zwei nahe verwandten, aber recht unterschiedlich gefärbten Arten. Acknowledgements This work would not have been successfully accomplished without the help of many collegues whose support and contributions I gratefully acknowledge: To my mentor Prof. Dr. Clas M. Naumann (Bonn) I am especially indebted. He initiated this work and I have to thank him for his continuous and generous support at the Zoologisches Forschungsinstitut und Museum Alexander Koenig and his sympathy in difficult situations of life. I was especially pleased to be able to accompagny him on an excursion to the core area of Agrodiaetus, where he also helped with the tedious work of preparations. Without his aid and his outstanding expertise this work would not have been accomplished. Prof. Dr. Konrad Fiedler (Bayreuth) brought in his excellent knowledge on Lycaenidae and thus was instrumental in initiating this project as well as bringing it to a satisfactory end. I am looking forward to future cooperation. Dr. habil. Bernhard Misof (Bonn) I have to thank for taking the task of Korreferat. His excellent supervision of laboratory work was indispensable for sequencing such a great amount of material. Dr. Jurate De Prins (Antwerpen) instructed me in her excellent method of karyological preparations and helped in preparing and analyzing numerous karyological slides. The hospitality of herself and her husband, Willy De Prins, during the many visits to Antwerp was extraordinary, and I am grateful for their friendship and two extremely successful joint expeditions to Anatolia. Dr. Vladimir Lukhtanov (St. Petersburg), the leading expert on Agrodiaetus karyology, demonstrated his new method of two-phase chromosome preparation as a visiting scientist to the Museum Koenig. He supported me with the preparation and analysis of a great number of preparations. I am greatful for his cordial cooperation. Karen Meusemann (Bonn) did a major proportion of chromosome preparations in the course of her diploma thesis on the karyology of Agrodiaetus. Thanks to her unusual efforts it was possible to analyze such a lot of preparations, and our discussions were helpful for both of us. I thank my other student assistents, Esther Meyer, Ruth Rottscheidt, Meike Thomas and Manuela Brenk for their dedicated support with molecular lab work. Manuela also helped in the production of several diagrams. Without this help the work would not have been completed yet. Most helpful was the cooperation of the other members of the Museum’s lab crew. Above all I have to thank Dr. Axel Hille, the first head of lab, for his introduction, Rainer Sonnenberg and Anja Schunke for further tutoring and numerous helps during the whole project and Oliver Niehuis for excellent cooperation in sequencing and the organization of the joint excursion to Morocco. 121 Acknowledgements Claudia Etzbauer, the soul of our lab, secured excellent working conditions, and with her sense of humour helped in frustrating periods of work. I thank the lepidopterists of the Museum Koenig, Dr. Dieter Stüning, Dr. Wolfgang Speidel and Dr. Harald Fänger for friendly support and numerous hints. Dirk van der Poorten & Alain Olivier (Antwerpen) helped to identify most of the Anatolian material and organized the two most successful joint expeditions to Central and Eastern Turkey. Dr. Wolfgang Eckweiler (Frankfurt), the leading expert of Iranian Agrodiaetus, contributed valuable material, and I am grateful for his help in identifying Iranian material and many inspiring discussions. Dr. Klaus G. Schurian (Sulzbach/Taunus) also helped in the identification of Agrodiaetus from Iran. Thank you for your friendship and interesting discussions. Dr. Sigbert Wagener (Oberhausen) magnanimously lent me his representative Agrodiaetus collection from Anatolia and explained his ideas on the evolution of Agrodiaetus. Dr. Alexandre Dantchenko (Moskow) supported me with material from Armenia. I thank him for interesting discussions during his time as visiting scholar to the Museum Koenig. John Coutsis (Athens) provided important Agrodiaetus-material from Greece and I also thank him for most interesting information on the genital morphology of Agrodiaetus. He also did preparations and excellent genitalia drawings from my material. An evaluation of this material was not possible for this thesis but will be done in a forthcoming publication of John and further joint work. Dr. José Munguira (Madrid) not only provided material from Spain but also helped with information on Spanish localities of Agrodiaetus. With Dr. Otakar Kudrna (Schweinfurt) I had interesting discussions and I thank him for providing two important Lysandra taxa for this project. Dr. Emilio Balletto (Torino) was very helpful when my car broke down during a visit to Torino on the excursion to Italy and I am greatful for his hospitality. Dr. Hendrik-Jan Megens (Leiden) supplied information on PCR-primers and a copy of his excellent thesis. Sven Erlacher (Jena) also helped with information on PCR primers for ND1. Thank you! My loving wife Paula not only helped in looking after our child and the household while I worked long hours in the lab. 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Iris 46:157-191. 142 Hilfsmittel & Erklärung Hilfsmittel Die Arbeit wurde auf einem PC mit MS Word 2000 verfasst. Die Tabellen wurden in MS Excel 2000 erstellt und für die Bearbeitung von Graphiken wurde MS Photo Editor und Adobe Photoshop Elements verwendet. Statistische Berechnungen erfolgten mit Analyse-It V.1.6.8. Für die molekulargenetische Auswertung kamen folgende Computerprogramme zum Einsatz: BioEdit V.5.0.9, ClustalX V.1.8.1, ForCon V.1.0, Mega V.1.0, V.2.1 & V.3.0 beta, MrBayes V.3.0b4, PAUP V.4.0 beta10, TCS V.1.13 und TreeView V.1.6.6. Erklärung Ich versichere, dass ich diese Arbeit selbständig verfasst, keine anderen Quellen und Hilfsmittel als die angegebenen benutzt und die Stellen der Arbeit, die anderen Werken dem Wortlaut oder dem Sinn nach entnommen sind, kenntlich gemacht habe. Die Arbeit hat in gleicher oder ähnlicher Form keiner anderen Prüfungsbehörde vorgelegen. Bonn, den 28. September 2003 143 144 Karyological result s in Agrodiaet us Appendix 1 Code WE WE MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW 02491 02492 98162 98166 98057 98082 98245 01003 01004 01054 00229 00231 98212 98315 99299 99380 99165 99240 99253 99338 99353 99376 99377 99378 99389 99393 99394 99403 99406 99058 99064 99372 00060 00061 00062 00068 00069 00070 00071 00072 00102 00103 00115 00267 00444 00445 00450 00457 00463 00476 00480 00482 00485 00487 00503 00547 00335 00409 00421 99060 99094 99375 99448 99475 99274 99276 99278 99319 99320 99471 00014 00015 00183 00185 00186 00189 99102 99105 Genus Species Chromosomes Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus achaemenes achaemenes actis actis admetus admetus admetus ainsae ainsae ainsae alcestis alcestis alcestis alcestis alcestis alcestis altivagans altivagans altivagans altivagans altivagans antidolus antidolus antidolus antidolus antidolus antidolus antidolus antidolus artvinensis artvinensis baytopi birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii caeruleus caeruleus caeruleus carmon cyaneus cyaneus cyaneus cyaneus dantchenkoi dantchenkoi dantchenkoi dantchenkoi dantchenkoi dantch.Xmenal. demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi many (atypical) many (atypical) n=ca.17 n=17 n=80 n=ca.73-80 n=78-80 n=100-108 n=ca.108 n=ca.108 n=ca.19 n=ca.19 n=21 n=20 n=19 n=19 n=ca.21 n=21 n=ca.23 n=ca.21 n=21-22 n>30 n=42 n>40 n=40-42 n=38-43 n=42 n=ca.40 n=ca.44 n=20-25 n=21-22 n=27-28 n=11 2n=ca.21 2n=20-22 n=11 2n=ca.20 2n=20-22 2n=22 2n=20-22 n=11 n=11 n=10-11 n=10 n=11 2n=20 2n=20-22 2n=20-22 2n=20-22 2n=20-22 2n=ca.20 2n=ca.20 2n=ca.20 2n=ca.20 2n=20 n=10 n=20 n=20 n=19-20 n>=79 n=17 n=18 n=18 n=18-19 n=40-42 n=40-43 n=40-45 n=42 n=40-41 n=45-50 n>=68 n=65-75 n=ca.70-80 n=many n=many n=many n>50 n=66 Date 21.07.2002 21.07.2002 25.07.1998 25.07.1998 13.07.1998 15.07.1998 29.07.1998 17.07.2001 17.07.2001 20.07.2001 16.07.2000 16.07.2000 28.07.1998 06.08.1998 18.07.1999 20.07.1999 15.07.1999 17.07.1999 17.07.1999 19.07.1999 19.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 21.07.1999 21.07.1999 22.07.1999 22.07.1999 08.07.1999 08.07.1999 19.07.1999 11.07.2000 11.07.2000 11.07.2000 11.07.2000 11.07.2000 11.07.2000 11.07.2000 11.07.2000 12.07.2000 12.07.2000 12.07.2000 18.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 28.07.2000 28.07.2000 28.07.2000 28.07.2000 28.07.2000 31.07.2000 04.08.2000 21.07.2000 23.07.2000 23.07.2000 08.07.1999 11.07.1999 20.07.1999 23.07.1999 25.07.1999 17.07.1999 17.07.1999 17.07.1999 18.07.1999 18.07.1999 25.07.1999 09.07.2000 09.07.2000 15.07.2000 15.07.2000 15.07.2000 15.07.2000 11.07.1999 11.07.1999 Locality Altitude Province Country Gardaneh ye Cheri Gardaneh ye Cheri Gökpinar Gökpinar Camkuyuzu Cukurelma Saimbeyli-Fälle Ilarduya Ilarduya Sta. Maria Qazayd Dagh Qazayd Dagh Saimbeyli-Fälle Yellibeli Geçidi Çatak Yüksekova Çaglayan Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Yüksekova Yüksekova Yüksekova Dilezi Geçidi Haruna Geçidi Haruna Geçidi Dez Çay Dez Çay Kiliçkaya Kiliçkaya Güzeldere Geç. Pul-e Zanguleh Pul-e Zanguleh Pul-e Zanguleh Gardaneh-ye Lavashm Gardaneh-ye Lavashm Gardaneh-ye Lavashm Gardaneh-ye Lavashm Gardaneh-ye Lavashm Dizin Pass Dizin Pass Shemshak Veresk Polur Polur Polur Polur Polur Golestanak Golestanak Golestanak Golestanak Golestanak Takht-e Suleyman Dizin Pass Shakuh Hajiabad Hajiabad Kiliçkaya Kagizman Zernek Brj. Zernek Brj. Erek Dagi Kurubas Geçidi Kurubas Geçidi Kurubas Geçidi Çatak Çatak Erek Dagi Samqabad Samqabad Dugijan Dugijan Dugijan Dugijan Akçay Akçay 2800-3000m 2800-3000m 1700 m 1700 m 1750 m 1300 m 1200-1500 m 550 m 550 m 500 m 2300 m 2300 m 1500 m 1800 m 1600-1900 m 1800 m 1500 m 2500 m 2500 m 2600 m 2500 m 1800 m 1800 m 1800 m 2100 m 2000 m 2000 m 1500 m 1500 m 1350 m 1350 m 2500 m 2400 m 2400 m 2400 m 2900 m 2900 m 2900 m 2900 m 2900 m 3000 m 3000 m 2900 m 1800-1950 m 2200 m 2200 m 2200 m 2200 m 2200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 3000 m 3200-3300 m 2600 m 2150 m 2150 m 1350 m 1400 m 1900 m 1900 m 2200 m 2200 m 2200 m 2200 m 2000-2200 m 2000-2200 m 2200 m 1900-2100 m 1900-2100 m 2000 m 2000 m 2000 m 2000 m 1200 m 1200 m Bakhtiari Bakhtiari Sivas Sivas Antalya Antalya Adana Alava Alava Huesca Zanjan Zanjan Adana Karaman Van Hakkari Erzincan Van Van Van Van Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Artvin Artvin Van Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Tehran Tehran Tehran Mazandaran Tehran Tehran Tehran Tehran Tehran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Tehran Golestan Golestan Golestan Artvin Kars Van Van Van Van Van Van Van Van Van Tehran Tehran Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Kars Kars Iran Iran Turkey Turkey Turkey Turkey Turkey Spain Spain Spain Iran Iran Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Iran Iran Iran Iran Iran Iran Turkey Turkey - 145 - Karyological result s in Agrodiaet us Appendix 1 Code MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW WE WE MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW WE MW MW MW MW MW MW WE MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW 99110 99142 99148 99150 99152 99382 99383 99384 99386 99391 99392 99405 99407 00539 00051 00052 00057 00058 00059 00065 00107 00110 00232 00316 98097 00393 00406 01036 01039 00226 00227 00228 02671 02672 00216 00217 00218 00234 99006 99007 99172 99241 99247 99413 99417 99422 99441 99443 99444 99457 01106 01107 00129 00177 02675 98293 98297 98298 00004 00005 00032 02676 98189 98190 98191 99408 99453 99053 99095 99552 99590 99591 99595 99596 99164 98101 98102 98104 Genus Species Chromosomes Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi dizinensis elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus ernesti erschoffii erschoffii fabressei fabressei femininoides femininoides femininoides femininoides femininoides firdussii firdussii firdussii firdussii firdussii firdussii firdussii firdussii firdussii firdussii firdussii firdussii firdussii firdussii firdussii firdussii fulgens fulgens gorbunovi gorbunovi gorbunovi guezelmavi guezelmavi guezelmavi hamadanensis hamadanensis hamadanensis hamadanensis hopfferi hopfferi hopfferi hopfferi hopfferi huberti huberti huberti humedasae humedasae humedasae humedasae interjectus iphicarmon iphicarmon iphicarmon n=55-70 n=ca.66 n=57-65 n=64-76 n>30 n=67-71 n=59 n=67 n=many n=many n=many n=many n>60 n=17 n=17 2n>=28 n=ca.17 n=18 n=16 n=17 n=ca.15-20 n=17-18 n=18 n=16-18 n=18 n=ca.14 n=ca.13-14 n=many n>75 n=27 n=ca.27 n=27 n=27 n=27 n=ca.29 n=29 n=28 n>=24 n=ca.30 n=28-31 n=32 n=25 n=ca.24 n=25 n=26 n=ca.25 n=25 n=27 n=ca.25 n=26 n=ca.98-103 n>90 n=20 n=ca.20 n=19 n=43 n=ca.40-43 n=ca.40 n=22 n=ca.22 n=ca.22 n=21 n=15-16 n=15-16 2n=30 n=15 n=15 n=36 n=33-34 n=ca.33 2n=ca.70-80 n=ca.38 2n>66 2n=ca.76 n=31 n=ca.30 n=ca.30 n=29 Date 11.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 22.07.1999 22.07.1999 04.08.2000 11.07.2000 11.07.2000 11.07.2000 11.07.2000 11.07.2000 11.07.2000 12.07.2000 12.07.2000 16.07.2000 19.07.2000 21.07.1998 23.07.2000 23.07.2000 19.07.2001 19.07.2001 16.07.2000 16.07.2000 16.07.2000 31.07.2002 31.07.2002 16.07.2000 16.07.2000 16.07.2000 16.07.2000 05.07.1999 05.07.1999 15.07.1999 17.07.1999 17.07.1999 22.07.1999 22.07.1999 22.07.1999 23.07.1999 23.07.1999 23.07.1999 24.07.1999 23.07.2001 23.07.2001 13.07.2000 15.07.2000 31.07.2002 04.08.1998 04.08.1998 04.08.1998 09.07.2000 09.07.2000 10.07.2000 31.07.2002 27.07.1998 27.07.1998 27.07.1998 22.07.1999 24.07.1999 08.07.1999 11.07.1999 29.07.1999 14.08.1999 14.08.1999 14.08.1999 14.08.1999 14.07.1999 21.07.1998 21.07.1998 21.07.1998 Locality Altitude Province Country Akçay Akçay Akçay Akçay Akçay Yüksekova Yüksekova Yüksekova Yüksekova Dilezi Geçidi Dilezi Geçidi Dez Çay Dez Çay Dizin Pass Nesa Nesa Kendevan Pul-e Zanguleh Pul-e Zanguleh Gardaneh-ye Lavashm Shahrestanak Shahrestanak Qazayd Dagh Asadbar Dedegöl Geçidi Hajiabad Hajiabad Abejar Abejar Qazayd Dagh Qazayd Dagh Qazayd Dagh Khalkhal, Gollijeh Khalkhal, Gollijeh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Çaglayan Çaglayan Çaglayan Güzeldere Geç. Güzeldere Geç. Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Çatak Sta. Coloma de Queralt Sta. Coloma de Queralt Ahar Pass Dugijan Khalkhal, Gollijeh Taskent Taskent Taskent Samqabad Samqabad Safedabad Khalkhal, Gollijeh Gündüzbey Gündüzbey Gündüzbey Dez Çay Çatak Kiliçkaya Kagizman Kop Geçidi Pondel Pondel Pondel Pondel Çiftlik Dedegöl Geçidi Dedegöl Geçidi Dedegöl Geçidi 1200 m 1200 m 1200 m 1200 m 1200 m 1800 m 1800 m 1800 m 1800 m 2100 m 2100 m 1500 m 1500 m 3200-3300 m 2100 m 2100 m 2150 m 2400 m 2400 m 2900 m 2000 m 2000 m 2300 m 2500 m 1700 m 2150 m 2150 m 1100 m 1100 m 2300 m 2300 m 2300 m 1900m 1900m 2300 m 2300 m 2300 m 2300 m 1500 m 1500 m 1500 m 2500 m 2500 m 1500 m 1500 m 1500 m 1500-1700 m 1500-1700 m 1500-1700 m 1600-1900 m 700 m 700 m 1800-1850 m 2000 m 1900m 1450 m 1450 m 1450 m 1900-2100 m 1900-2100 m 2000 m 1900m 1300 m 1300 m 1300 m 1500 m 1600-1900 m 1350 m 1400 m 2350 m 900 m 900 m 900 m 900 m 1900 m 1700 m 1700 m 1700 m Kars Kars Kars Kars Kars Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Tehran Tehran Tehran Mazandaran Mazandaran Mazandaran Mazandaran Tehran Tehran Zanjan Tehran Isparta Golestan Golestan Soria Soria Zanjan Zanjan Zanjan Ardabil Ardabil Zanjan Zanjan Zanjan Zanjan Erzincan Erzincan Erzincan Van Van Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Van Tarragona Tarragona Azarbayjan-e Sharqi Azarbayjan-e Sharqi Ardabil Konya Konya Konya Tehran Tehran Tehran Ardabil Malatya Malatya Malatya Hakkari Van Artvin Kars Bayburt Aosta Aosta Aosta Aosta Erzurum Isparta Isparta Isparta Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Turkey Iran Iran Spain Spain Iran Iran Iran Iran Iran Iran Iran Iran Iran Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Spain Spain Iran Iran Iran Turkey Turkey Turkey Iran Iran Iran Iran Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Italy Italy Italy Italy Turkey Turkey Turkey Turkey - 146 - Karyological result s in Agrodiaet us Appendix 1 Code MW MW MW MW MW MW MW MW MW MW MW MW MW WE WE MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW WE WE WE MW MW MW MW MW MW MW WE WE WE MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW 00269 00274 00287 00338 00362 00381 00424 98049 98106 99009 99154 99170 99465 02611 02612 00259 00261 00262 00264 99286 99287 99288 99473 98069 98070 98071 98072 98170 98022 98064 99496 99057 99059 98203 02453 02454 02614 99508 00126 00127 00155 00157 00158 00161 02591 02592 02593 00236 00307 00348 00355 00452 99292 99341 99416 98137 98138 98141 98144 98145 98154 98178 98180 98183 00347 00330 99061 99501 01014 01015 98100 98215 99068 99196 99218 99219 99220 99263 Genus Species Chromosomes Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphigenia iphigenia iphigenia iphigenia iphigenia kanduli karindus karindus klausschuriani klausschuriani klausschuriani klausschuriani kurdistanicus kurdistanicus kurdistanicus kurdistanicus lycius lycius lycius lycius maraschi menalcas menalcas menalcas merhaba merhaba mithridates mofidii mofidii morgani ninae paulae paulae paulae paulae paulae paulae peilei peilei peilei phyllis phyllis phyllis phyllis phyllis pierceae pierceae pierceae poseidon poseidon poseidon poseidon poseidon poseidon poseidon poseidon poseidon posthumus pseudoxerxes putnami putnami ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii n=ca.14 n=14 n=14 n=ca.14 n=14 n=14 n=13-15 n=14 n=15 n=12 n=11-14 n=12 n=25 n=68 n>=66 n=56-58 n>47 n=56 n=56 n=54-56 n=56-68 n=62 n>=55 n=21-22 n=21-22 n=22 n=21-22 n=16 n=85 n>75 n=85 n=ca.17 n=15-17 n=23 n=ca.45 2n>80 n=27 n=34 n=17 n=17 n=ca.18 n=ca.17 n=ca.17 n=17 n=39 n=39 n=39 n=many n=76-78 n=82-86 n=many n>75 n=21 n=22-24 n=22 n=21 n=20-21 n=21 n=21 n=20 n=21 n=19 n=19 n=19 n=ca.85 n=15 n=ca.25 n=25 n=ca.90 n=ca.90 n>80 n=ca.90 n=ca.90 n>85 n=many n=many n=many n>71 Date 18.07.2000 18.07.2000 18.07.2000 21.07.2000 21.07.2000 21.07.2000 23.07.2000 12.07.1998 21.07.1998 05.07.1999 13.07.1999 15.07.1999 24.07.1999 27.07.2002 27.07.2002 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.1999 18.07.1999 18.07.1999 25.07.1999 15.07.1998 15.07.1998 15.07.1998 15.07.1998 25.07.1998 12.07.1998 13.07.1998 25.07.1999 08.07.1999 08.07.1999 27.07.1998 17.07.2002 17.07.2002 27.07.2002 26.07.1999 13.07.2000 13.07.2000 13.07.2000 13.07.2000 13.07.2000 13.07.2000 27.07.2002 27.07.2002 27.07.2002 16.07.2000 19.07.2000 21.07.2000 21.07.2000 26.07.2000 18.07.1999 19.07.1999 22.07.1999 22.07.1998 22.07.1998 22.07.1998 22.07.1998 22.07.1998 22.07.1998 25.07.1998 25.07.1998 25.07.1998 21.07.2000 21.07.2000 08.07.1999 26.07.1999 18.07.2001 18.07.2001 21.07.1998 28.07.1998 08.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 17.07.1999 Locality Altitude Province Country Veresk Veresk Veresk Shakuh Shakuh Shakuh Hajiabad Salur Dagi Dedegöl Geçidi Çaglayan Kagizman Çaglayan Çatak 40 km SW Saqqez 40 km SW Saqqez Veresk Veresk Veresk Veresk Çatak Çatak Çatak Erek Dagi Cukurelma Cukurelma Cukurelma Cukurelma Gökpinar Gülübeli Geçidi Camkuyuzu Erek Dagi Kiliçkaya Kiliçkaya Gündüzbey Kuh-e-Sorkh, Kadkan Kuh-e-Sorkh, Kadkan 40 km SW Saqqez Agrı Ahar Pass Ahar Pass Ahar Pass Ahar Pass Ahar Pass Ahar Pass Qamchiyan Qamchiyan Qamchiyan Qazayd Dagh Asadbar Shakuh Shakuh Polur Çatak Güzeldere Geç. Dez Çay Zelve Zelve Zelve Zelve Zelve Zelve Gökpinar Gökpinar Gökpinar Shakuh Shakuh Kiliçkaya Agrı Ubierna Ubierna Dedegöl Geçidi Saimbeyli-Fälle Kiliçkaya Çaglayan Çaglayan Çaglayan Çaglayan Kurubas Geçidi 1800-1950 m 1800-1950 m 1800-1950 m 2600 m 2600 m 3000 m 2150 m 1700-1900 m 1700 m 1500 m 1400 m 1500 m 1600-1900 m 1800-1900m 1800-1900m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1600-1900 m 1600-1900 m 1600-1900 m 2200 m 1300 m 1300 m 1300 m 1300 m 1700 m 1500 m 1750 m 2200 m 1350 m 1350 m 1300 m 2100-2500m 2100-2500m 1800-1900m 1800 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1900m 1800-1900m 1800-1900m 2300 m 2900 m 2600 m 2600 m 2200 m 1600-1900 m 2600 m 1500 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1700 m 1700 m 1700 m 2600 m 2600 m 1350 m 1800 m 900 m 900 m 1700 m 1500 m 1350 m 1500 m 1500 m 1500 m 1500 m 2200 m Mazandaran Mazandaran Mazandaran Golestan Golestan Golestan Golestan Fethiye Isparta Erzincan Kars Erzincan Van Kordestan Kordestan Mazandaran Mazandaran Mazandaran Mazandaran Van Van Van Van Antalya Antalya Antalya Antalya Sivas Fethiye Antalya Van Artvin Artvin Malatya Khorasan Khorasan Kordestan Agrı Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Kordestan Kordestan Kordestan Zanjan Tehran Golestan Golestan Tehran Van Van Hakkari Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Sivas Sivas Sivas Golestan Golestan Artvin Agrı Burgos Burgos Isparta Adana Artvin Erzincan Erzincan Erzincan Erzincan Van Iran Iran Iran Iran Iran Iran Iran Turkey Turkey Turkey Turkey Turkey Turkey Iran Iran Iran Iran Iran Iran Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Iran Iran Iran Turkey Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Iran Iran Turkey Turkey Spain Spain Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey - 147 - Karyological result s in Agrodiaet us Appendix 1 Code MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW MW WE WE 99574 98261 99289 98305 98313 98279 98285 98240 98241 98243 99258 99262 99272 99273 99294 99314 99321 99464 99478 99479 99483 99135 99189 99203 00498 98136 98139 99228 99229 99373 99374 99476 02531 02533 Genus Species Chromosomes Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus ripartii schuriani sekercioglui sertavulensis sertavulensis sigberti sigberti theresiae theresiae theresiae turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicus turcicus turcicus valiabadi wagneri wagneri zapvadi zapvadi zapvadi zapvadi zapvadi zarathustra zarathustra n=many n=ca.75-80 n>=46 n=20 n=ca.20 n=28 n=25 n=59 n=ca.59 n=63 n=20 n=20 n=ca.18-22 n=ca.18-22 n=22 n=ca.20 n=20 n=20 n=19 n=20 n=19-20 2n=ca.50 n=ca.24 n=22-24 n=23 n=18 n=19/21 n=18 n=ca.17 n=18 n=19 n=18-20 n=ca.22 n=ca.22 Date 29.07.1999 30.07.1998 18.07.1999 06.08.1998 06.08.1998 31.07.1998 31.07.1998 29.07.1998 29.07.1998 29.07.1998 17.07.1999 17.07.1999 17.07.1999 17.07.1999 18.07.1999 18.07.1999 18.07.1999 24.07.1999 25.07.1999 25.07.1999 25.07.1999 12.07.1999 15.07.1999 15.07.1999 30.07.2000 22.07.1998 22.07.1998 17.07.1999 17.07.1999 20.07.1999 20.07.1999 25.07.1999 25.07.2002 25.07.2002 Locality Altitude Province Country Kop Geçidi Gezbeli Geçidi Çatak Yellibeli Geçidi Yellibeli Geçidi Ala Daglar Ala Daglar Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Kurubas Geçidi Kurubas Geçidi Kurubas Geçidi Kurubas Geçidi Çatak Çatak Çatak Çatak Erek Dagi Erek Dagi Erek Dagi Badilli Çaglayan Çaglayan 5 km S Valiabad Zelve Zelve Zernek Brj. Zernek Brj. Zernek Brj. Zernek Brj. Erek Dagi 30 km W Dorud 30 km W Dorud 2350 m 1800 m 1600-1900 m 1800 m 1800 m 2900 m 2700 m 1200-1500 m 1200-1500 m 1200-1500 m 2200 m 2200 m 2200 m 2200 m 1600-1900 m 2000-2200 m 2000-2200 m 1600-1900 m 2200 m 2200 m 2200 m 1800-2000 m 1500 m 1500 m 1900 m 1100 m 1100 m 1900 m 1900 m 1900 m 1900 m 2200 m 2100m 2100m Bayburt Kayseri Van Karaman Karaman Kayseri Kayseri Adana Adana Adana Van Van Van Van Van Van Van Van Van Van Van Igdir Erzincan Erzincan Mazandaran Nevsehir Nevsehir Van Van Van Van Van Lorestan Lorestan Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Iran Turkey Turkey Turkey Turkey Turkey Turkey Turkey Iran Iran Chromosome numbers in bold type are new for this taxon. - 148 - Appendix 2 Code AD 98001 AD 98002 AD 98003 AD 98004 AD 98005 AD 98006 AD 98007 AD 98008 AD 98009 AD 98010 AD 98011 AD 98012 AD 98013 AD 98014 AD 98015 AD 98016 AD 98017 AD 98018 AD 98019 AD 98020 AD 98021 AD 98022 AD 98023 AD 98024 AD 98025 AD 98026 AD 98027 AD 98028 AD 98029 AD 98030 AD 98031 AD 98032 AD 98033 AD 98034 AD 98035 AD 98036 AD 98037 AD 98038 AD 98039 AD 98040 AD 98041 AD 98042 AD 98043 AD 98044 AD 98045 AD 98046 AD 98047 AD 98048 AD 98049 DS 00001 DS 00002 DS 00003 DS 00004 DS 00005 DS 01001 DS 95001 DS 99001 DS 99002 JC 00029 JC 00038 JC 00039 JC 00040 JC 00041 JC 00042 JC 00043 JC 00044 JC 00045 JC 00046 JC 00047 JC 00048 JC 00051 JC 00053 JC 00054 JC 00055 JC 00056 JC 00057 JC 00060 JC 00061 JC 00062 JC 00063 JC 00064 JC 00067 JC 00070 JC 00072 JC 00074 JC 01014 JC 01018 JC 01020 JC 01027 JC 01033 JC 01038 JC 01039 JC 02001 JC 02002 JC 02003 JC 02004 JC 02005 JM 00001 JM 00002 JM 00003 JM 00004 MW 00001 MW 00002 MW 00003 MW 00004 MW 00005 MW 00006 MW 00007 MW 00008 MW 00009 MW 00010 MW 00011 MW 00012 MW 00013 MW 00014 Sex ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Agrodiaetus Aricia Aricia Aricia Polyommatus Polyommatus Aricia Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Agrodiaetus Aricia Agrodiaetus Coenonympha Coenonympha Coenonympha Coenonympha Iphiclides Pontia Pontia Pontia Pontia Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Species Wgs Fix surakovi 1 surakovi 1 surakovi 1 surakovi 1 surakovi 1 surakovi 1 surakovi 1 surakovi 1 pseudactis 1 pseudactis 1 pseudactis 1 altivagans 1 altivagans 1 altivagans 1 altivagans 1 altivagans 1 altivagans 1 ninae 1 ninae 1 ninae 1 ninae 1 ninae 1 ninae 1 huberti 1 1 huberti huberti 1 1 huberti 1 huberti iphigenia 1 iphigenia 1 iphigenia 1 iphigenia 1 iphigenia 1 iphigenia 1 iphigenia 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 phyllis 1 poseidonides 1 1 damone 1 damocles 1 phyllides dagmara 1 iphigenides 1 1 actinides 1 damone 1 transcaspicus menelaos 1 menelaos 1 escheri 1 aroaniensis 1 aroaniensis 1 eroides 1 ripartii 1 aroaniensis 1 nephohiptamenos 1 nephohiptamenos 1 aroaniensis 1 nephohiptamenos 1 menelaos 1 escheri 1 ripartii 1 artaxerxes 1 artaxerxes 1 artaxerxes 1 menelaos 1 andronicus 1 agestis 1 icarus 1 andronicus 1 icarus 1 andronicus 1 andronicus 1 andronicus 1 admetus 1 agestis admetus arcania arcania arcania arcania podalirius daplidice daplidice daplidice daplidice fabressei 1 fabressei 1 fabressei 1 fabressei 1 hamadanensis 1C hamadanensis 1C hamadanensis 1C hamadanensis 1C hamadanensis 1C hamadanensis 1C hamadanensis 1C hamadanensis 1C demavendi 1C demavendi 1C demavendi 1C demavendi 1C demavendi 1C demavendi 1C List of m at erial Kar Alc 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 P P P P P P P 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 DNA Cytb CO1 ND1 ITS2 Fig. Date 1 1 1 I-1 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 1 1 1 I-2 20.07.1998 20.07.1998 20.07.1998 1 1 1 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 1 1 1 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 1 1 1 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 1 1 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 1 1 1 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 20.07.1998 1 1 1 I-3 23.06.2000 1 I-4 03.07.2000 1 I-5 05.07.2000 1 I-6 15.06.2000 1 1 I-7 23.06.2000 1 1 1 I-8 08.06.2001 1 I-9 24.07.1995 1 12.07.1999 1 03.07.1999 1 1 1 16.06.2000 16.06.2000 1 1 1 09.07.2000 1 1 1 I-10 04.07.2000 04.07.2000 1 1 1 08.07.2000 1 1 1 21.06.2000 04.07.2000 1 1 1 I-11 07.07.2000 1 1 07.07.2000 04.07.2000 07.07.2000 1 1 1 09.07.2000 09.07.2000 21.06.2000 1 1 1 16.06.2000 16.06.2000 1 1 1 16.06.2000 16.06.2000 1 1 1 09.07.2000 1 1 09.07.2000 1 1 1 09.07.2000 07.07.2000 09.07.2000 09.07.2000 09.07.2000 07.07.2000 1 1 1 I-12 14.06.2001 14.06.2001 14.06.2001 08.07.2001 08.07.2001 08.07.2001 08.07.2001 1 1 06.09.2002 06.09.2002 06.09.2002 06.09.2002 06.09.2002 1 1 1 I-13 18.07.2000 18.07.2000 18.07.2000 18.07.2000 1 1 09.07.2000 09.07.2000 09.07.2000 09.07.2000 09.07.2000 09.07.2000 09.07.2000 09.07.2000 09.07.2000 09.07.2000 09.07.2000 09.07.2000 09.07.2000 09.07.2000 - 149 - Locality Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Gnyshik village Safedou Kuvandyk Kuvandyk Kitabsky national reserve Nikolaevsky Pass Kitabsky national reserve Transalaisky Mts. Kuray Nohur Mt. Taiyetos Mt. Taiyetos Mt. Falakro Mt. Helmos Mt. Helmos Rodopi Mts. Mt. Helmos Mt. Helmos Mt. Orvilos Mt. Orvilos Mt. Helmos Mt. Orvilos Mt. Falakro Mt. Falakro Mt. Helmos Mt. Taiyetos Mt. Taiyetos Mt. Taiyetos Mt. Taiyetos Mt. Falakro Mt. Falakro Mt. Falakro Mt. Orvilos Mt. Falakro Mt. Falakro Mt. Falakro Mt. Orvilos Mt. Taiyetos Mt. Taiyetos Mt. Taiyetos Vathirema Vathirema Vathirema Vathirema Agia Marina Agia Marina Agia Marina Agia Marina Agia Marina Mogorrita Mogorrita Mogorrita Mogorrita Samqabad Samqabad Samqabad Samqabad Samqabad Samqabad Samqabad Samqabad Samqabad Samqabad Samqabad Samqabad Samqabad Samqabad Area Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Aiotzdzorsky range Darvaz Mts. South Urals South Urals Zeravshansky Mts. Khozratishoh Mts. Zeravshansky Mts. Aram-Kungei Range Tadzhilu riv. Kopet Dag Rhodopi Mts. Rhodopi Mts. Rhodopi Mts. Rhodopi Mts. Spetses Island Spetses Island Spetses Island Spetses Island Spetses Island Tragacete Tragacete Tragacete Tragacete nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek Altitude 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 1800-2200 m 2500 m 2000 m Province Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia Transcaucasia 1180-1200 m 1180-1200 m 1650 m 1350 m 1350 m 1200 m 1350-1500 m 1350 m 1200-2100 m 1200-2100 m 1350 m 1200-2100 m 1650 m 1650 m 1350-1500 m 1180-1200 m 1180-1200 m 1180-1200 m 1180-1200 m 1650 m 1650 m 1650 m 1200-2100 m 1650 m 1650 m 1650 m 1200-2100 m 1200-1300 m 1200-1300 m 1200-1300 m 1000 m 1000 m 1000 m 1000 m 0m 0m 0m 0m 0m Peloponnisos Peloponnisos Macedonia Peloponnisos Peloponnisos Macedonia Peloponnisos Peloponnisos Macedonia Macedonia Peloponnisos Macedonia Macedonia Macedonia Peloponnisos Peloponnisos Peloponnisos Peloponnisos Peloponnisos Macedonia Macedonia Macedonia Macedonia Macedonia Macedonia Macedonia Macedonia Peloponnisos Peloponnisos Peloponnisos Macedonia Macedonia Macedonia Macedonia Orenburg Orenburg 1500-2500 m 2500 m 1500-2500 m 3400-4400 m 1700-1800 m SE Altai Mts 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m Cuenca Cuenca Cuenca Cuenca Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Country Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Armenia Tajikistan Russia Russia Uzbekistan Tajikistan Uzbekistan Kirgizia Russia Turkmenia Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Greece Spain Spain Spain Spain Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Appendix 2 Code MW 00015 MW 00016 MW 00017 MW 00018 MW 00019 MW 00020 MW 00021 MW 00022 MW 00023 MW 00024 MW 00025 MW 00026 MW 00027 MW 00028 MW 00029 MW 00030 MW 00031 MW 00032 MW 00033 MW 00034 MW 00035 MW 00036 MW 00037 MW 00038 MW 00039 MW 00040 MW 00041 MW 00042 MW 00043 MW 00044 MW 00045 MW 00046 MW 00047 MW 00048 MW 00049 MW 00050 MW 00051 MW 00052 MW 00053 MW 00054 MW 00055 MW 00056 MW 00057 MW 00058 MW 00059 MW 00060 MW 00061 MW 00062 MW 00063 MW 00064 MW 00065 MW 00066 MW 00067 MW 00068 MW 00069 MW 00070 MW 00071 MW 00072 MW 00073 MW 00074 MW 00075 MW 00076 MW 00077 MW 00078 MW 00079 MW 00080 MW 00081 MW 00082 MW 00083 MW 00084 MW 00085 MW 00086 MW 00087 MW 00088 MW 00089 MW 00090 MW 00091 MW 00092 MW 00093 MW 00094 MW 00095 MW 00096 MW 00097 MW 00098 MW 00099 MW 00100 MW 00101 MW 00102 MW 00103 MW 00104 MW 00105 MW 00106 MW 00107 MW 00108 MW 00109 MW 00110 MW 00111 MW 00112 MW 00113 MW 00114 MW 00115 MW 00116 MW 00117 MW 00118 MW 00119 MW 00120 MW 00121 MW 00122 MW 00123 MW 00124 MW 00125 MW 00126 MW 00127 MW 00128 MW 00129 Sex ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♀ ♀ ♂ ♂ ♂ ♀ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♀ ♀ ♀ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Agrodiaetus Agrodiaetus Lycaena Polyommatus Polyommatus Aricia Lycaena Agrodiaetus Vacciniina Vacciniina Vacciniina Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Lycaena Lycaena Lycaena Lycaena Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Polyommatus Polyommatus Plebeius Lycaena Polyommatus Meleageria Meleageria Meleageria Meleageria Meleageria Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Plebeius Neolysandra Neolysandra Lycaena Lycaena Lycaena Vacciniina Neolysandra Neolysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Species demavendi demavendi alciphron icarus icarus agestis phlaeas hamadanensis alcedo alcedo alcedo hamadanensis hamadanensis hamadanensis hamadanensis demavendi hamadanensis hamadanensis hamadanensis hamadanensis hamadanensis hamadanensis hamadanensis icarus icarus icarus icarus icarus icarus asabinus alciphron phlaeas tityrus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus birunii birunii birunii birunii valiabadi elbursicus elbursicus elbursicus birunii birunii birunii birunii birunii birunii birunii icarus daphnis daphnis daphnis daphnis daphnis daphnis amandus amandus pylaon alciphron icarus marcida marcida marcida marcida marcida elbursicus elbursicus elbursicus elbursicus birunii birunii birunii phyllis birunii darius birunii birunii birunii elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus elbursicus birunii argus corona corona candens candens tityrus morgianus corona corona firdussii paulae paulae paulae gorbunovi List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C Kar Alc DNA Cytb CO1 ND1 ITS2 Fig. Date Locality P 1 1 1 1 I-14 09.07.2000 Samqabad P 1 09.07.2000 Samqabad 1 1 1 09.07.2000 Samqabad 1 09.07.2000 Samqabad 1 09.07.2000 Samqabad 1 1 1 09.07.2000 Samqabad 1 09.07.2000 Samqabad 1 09.07.2000 Samqabad 1 09.07.2000 Samqabad 1 1 1 1 09.07.2000 Samqabad 1 09.07.2000 Samqabad 1 09.07.2000 Samqabad 1 09.07.2000 Samqabad 1 09.07.2000 Samqabad 1 09.07.2000 Samqabad P 1 09.07.2000 Samqabad P 1 10.07.2000 Safedabad P 1 1 1 1 1 I-15 10.07.2000 Safedabad P 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 1 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad 1 10.07.2000 Safedabad P 1 11.07.2000 Nesa P 1 11.07.2000 Nesa P 1 11.07.2000 Nesa P 1 1 1 1 11.07.2000 Nesa P 1 11.07.2000 Nesa 1 11.07.2000 Nesa 1 11.07.2000 Nesa P 1 11.07.2000 Nesa P 1 1 1 1 11.07.2000 Kendevan P 1 11.07.2000 Kendevan P 1 1 1 11.07.2000 Pul-e Zanguleh P 1 1 1 1 I-16 11.07.2000 Pul-e Zanguleh P 1 1 1 11.07.2000 Pul-e Zanguleh P 1 11.07.2000 Pul-e Zanguleh P 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Pul-e Zanguleh P 1 1 1 1 11.07.2000 Pul-e Zanguleh P 1 11.07.2000 Gardaneh-ye Lavashm P 1 11.07.2000 Gardaneh-ye Lavashm 1 11.07.2000 Gardaneh-ye Lavashm P 1 11.07.2000 Gardaneh-ye Lavashm P 1 11.07.2000 Gardaneh-ye Lavashm P 1 11.07.2000 Gardaneh-ye Lavashm P 1 11.07.2000 Gardaneh-ye Lavashm P 1 1 1 1 1 11.07.2000 Gardaneh-ye Lavashm P 1 11.07.2000 Gardaneh-ye Lavashm 1 11.07.2000 Gardaneh-ye Lavashm 1 11.07.2000 Nesa 1 1 1 1 1 11.07.2000 Nesa 1 11.07.2000 Nesa 1 11.07.2000 Nesa 1 11.07.2000 Nesa 1 11.07.2000 Nesa 1 11.07.2000 Nesa 1 11.07.2000 Kendevan 1 11.07.2000 Kendevan 1 11.07.2000 Valiabad 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Pul-e Zanguleh 1 1 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Nesa 1 11.07.2000 Nesa 1 11.07.2000 Nesa 1 11.07.2000 Nesa 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Pul-e Zanguleh 1 11.07.2000 Pul-e Zanguleh P 1 1 1 1 I-17 12.07.2000 Dizin Pass P 1 1 1 1 12.07.2000 Dizin Pass P 1 12.07.2000 Dizin Pass 1 12.07.2000 Dizin Pass P 1 12.07.2000 Shahrestanak 1 12.07.2000 Shahrestanak P 1 12.07.2000 Shahrestanak 1 12.07.2000 Shahrestanak 1 12.07.2000 Shahrestanak P 1 1 1 1 12.07.2000 Shahrestanak P 1 12.07.2000 Shahrestanak P 1 12.07.2000 Gajereh 1 12.07.2000 Gajereh 1 12.07.2000 Gajereh P 1 12.07.2000 Shemshak 1 1 1 1 12.07.2000 Shemshak 1 12.07.2000 Shemshak 1 12.07.2000 Shemshak 1 1 1 12.07.2000 Shemshak 1 12.07.2000 Shemshak 1 12.07.2000 Shemshak 1 12.07.2000 Dizin Pass 1 12.07.2000 Dizin Pass 1 12.07.2000 Dizin Pass P 1 1 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass P 1 1 1 1 I-18 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass P 1 1 1 1 I-19 13.07.2000 Ahar Pass - 150 - Area nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek nordöstl. Abyek westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj westl. Sirud / Karaj 1 km N Nesa 1 km N Nesa 1 km N Nesa 1 km N Nesa 1 km N Nesa 1 km N Nesa 1 km N Nesa 1 km N Nesa 5 km N Kendevan 5 km N Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 20 km NO Kendevan 20 km NO Kendevan 20 km NO Kendevan 20 km NO Kendevan 20 km NO Kendevan 20 km NO Kendevan 20 km NO Kendevan 20 km NO Kendevan 20 km NO Kendevan 20 km NO Kendevan 1 km N Nesa 1 km N Nesa 1 km N Nesa 1 km N Nesa 1 km N Nesa 1 km N Nesa 1 km N Nesa 5 km N Kendevan 5 km N Kendevan nördl. Valiabad 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 1 km N Nesa 1 km N Nesa 1 km N Nesa 1 km N Nesa 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan 15 km NO Kendevan südl. Dizin südl. Dizin südl. Dizin südl. Dizin nördl. Tehran nördl. Tehran nördl. Tehran nördl. Tehran nördl. Tehran nördl. Tehran nördl. Tehran südl. Dizin Pass südl. Dizin Pass südl. Dizin Pass südl. Dizin Pass südl. Dizin Pass südl. Dizin Pass südl. Dizin Pass südl. Dizin südl. Dizin südl. Dizin 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar Altitude 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 1900-2100 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2100 m 2100 m 2100 m 2100 m 2100 m 2100 m 2100 m 2100 m 2150 m 2150 m 2400 m 2400 m 2400 m 2400 m 2400 m 2400 m 2400 m 2900 m 2900 m 2900 m 2900 m 2900 m 2900 m 2900 m 2900 m 2900 m 2900 m 2100 m 2100 m 2100 m 2100 m 2100 m 2100 m 2100 m 2150 m 2150 m 1600 m 2400 m 2400 m 2400 m 2400 m 2400 m 2400 m 2400 m 2100 m 2100 m 2100 m 2100 m 2400 m 2400 m 2400 m 2400 m 2400 m 3000 m 3000 m 3000 m 3000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2200 m 2200 m 2200 m 2900 m 2900 m 2900 m 2900 m 2900 m 2900 m 2900 m 3000 m 3000 m 3000 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m Province Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Tehran Tehran Tehran Tehran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Country Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Appendix 2 Code MW 00130 MW 00131 MW 00132 MW 00133 MW 00134 MW 00135 MW 00136 MW 00137 MW 00138 MW 00139 MW 00140 MW 00141 MW 00142 MW 00143 MW 00144 MW 00145 MW 00146 MW 00147 MW 00148 MW 00149 MW 00150 MW 00151 MW 00152 MW 00153 MW 00154 MW 00155 MW 00156 MW 00157 MW 00158 MW 00159 MW 00160 MW 00161 MW 00162 MW 00163 MW 00164 MW 00165 MW 00166 MW 00167 MW 00168 MW 00169 MW 00170 MW 00171 MW 00172 MW 00173 MW 00174 MW 00175 MW 00176 MW 00177 MW 00178 MW 00179 MW 00180 MW 00181 MW 00182 MW 00183 MW 00184 MW 00185 MW 00186 MW 00187 MW 00188 MW 00189 MW 00190 MW 00191 MW 00192 MW 00193 MW 00194 MW 00195 MW 00196 MW 00197 MW 00198 MW 00199 MW 00200 MW 00201 MW 00202 MW 00203 MW 00204 MW 00205 MW 00206 MW 00207 MW 00208 MW 00209 MW 00210 MW 00211 MW 00212 MW 00213 MW 00214 MW 00215 MW 00216 MW 00217 MW 00218 MW 00219 MW 00220 MW 00221 MW 00222 MW 00223 MW 00224 MW 00225 MW 00226 MW 00227 MW 00228 MW 00229 MW 00230 MW 00231 MW 00232 MW 00233 MW 00234 MW 00235 MW 00236 MW 00237 MW 00238 MW 00239 MW 00240 MW 00241 MW 00242 MW 00243 MW 00244 Sex ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♀ ♀ ♀ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♀ ♀ ♀ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♀ Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Plebeius Plebeius Polyommatus Satyrium Satyrium Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Polyommatus Polyommatus Polyommatus Polyommatus Meleageria Meleageria Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lycaena Lycaena Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Meleageria Meleageria Meleageria Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Species phyllis phyllis paulae phyllis phyllis phyllis phyllis phyllis phyllis phyllis phyllis phyllis firdussii firdussii firdussii firdussii alcestis phyllis icarus icarus firdussii firdussii firdussii firdussii paulae firdussii paulae paulae paulae paulae paulae paulae phyllis phyllis phyllis phyllis phyllis phyllis phyllis alcestis argus argus thersites abdominalis abdominalis rovshani gorbunovi gorbunovi cyaneus phyllis phyllis demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi bellargus icarus thersites icarus icarus daphnis daphnis demavendi demavendi phyllis phyllis phyllis phyllis phyllis phyllis icarus icarus phyllis phyllis phyllis phyllis phyllis thersamon thersamon firdussii firdussii firdussii firdussii firdussii phyllis phyllis phyllis daphnis daphnis daphnis femininoides femininoides femininoides alcestis alcestis alcestis elbursicus firdussii firdussii firdussii phyllis phyllis phyllis phyllis phyllis phyllis femininoides femininoides alcestis List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C Kar Alc DNA Cytb CO1 ND1 ITS2 Fig. Date Locality P 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 1 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass P 1 1 1 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass P 1 1 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass P 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass 1 13.07.2000 Ahar Pass P 1 1 1 1 I-20 15.07.2000 Dugijan P 1 1 1 1 15.07.2000 Dugijan P 1 1 1 1 15.07.2000 Dugijan P 1 1 1 1 I-21 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan P 1 15.07.2000 Dugijan P 1 1 1 15.07.2000 Dugijan P 1 15.07.2000 Dugijan P 1 1 1 1 15.07.2000 Dugijan P 1 1 1 15.07.2000 Dugijan P 1 15.07.2000 Dugijan P 1 15.07.2000 Dugijan P 1 1 1 1 1 15.07.2000 Dugijan P 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan P 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan P 1 15.07.2000 Dugijan P 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan P 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan 1 15.07.2000 Dugijan P 1 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh P 1 1 1 1 I-22 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh P 1 1 1 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh P 1 1 1 1 1 I-23 16.07.2000 Qazayd Dagh P 1 1 1 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh P 1 1 1 1 I-24 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh P 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh 1 16.07.2000 Qazayd Dagh - 151 - Area 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 20 km SW Ahar 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 30 km NO Marand 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan Altitude 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 1800-1850 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m Province Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Azarbayjan-e Sharqi Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Country Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Appendix 2 Code MW 00245 MW 00246 MW 00247 MW 00248 MW 00249 MW 00250 MW 00251 MW 00252 MW 00253 MW 00254 MW 00255 MW 00256 MW 00257 MW 00258 MW 00259 MW 00260 MW 00261 MW 00262 MW 00263 MW 00264 MW 00265 MW 00266 MW 00267 MW 00268 MW 00269 MW 00270 MW 00271 MW 00272 MW 00273 MW 00274 MW 00275 MW 00276 MW 00277 MW 00278 MW 00279 MW 00280 MW 00281 MW 00282 MW 00283 MW 00284 MW 00285 MW 00286 MW 00287 MW 00288 MW 00289 MW 00290 MW 00291 MW 00292 MW 00293 MW 00294 MW 00295 MW 00296 MW 00297 MW 00298 MW 00299 MW 00300 MW 00301 MW 00302 MW 00303 MW 00304 MW 00305 MW 00306 MW 00307 MW 00308 MW 00309 MW 00310 MW 00311 MW 00312 MW 00313 MW 00314 MW 00315 MW 00316 MW 00317 MW 00318 MW 00319 MW 00320 MW 00321 MW 00322 MW 00323 MW 00324 MW 00325 MW 00326 MW 00327 MW 00328 MW 00329 MW 00330 MW 00331 MW 00332 MW 00333 MW 00334 MW 00335 MW 00336 MW 00337 MW 00338 MW 00339 MW 00340 MW 00341 MW 00342 MW 00343 MW 00344 MW 00345 MW 00346 MW 00347 MW 00348 MW 00349 MW 00350 MW 00351 MW 00352 MW 00353 MW 00354 MW 00355 MW 00356 MW 00357 MW 00358 MW 00359 Sex ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Polyommatus Plebeius Plebeius Meleageria Meleageria Meleageria Meleageria Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Vacciniina Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Lysandra Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Vacciniina Plebeius Polyommatus Aricia Meleageria Meleageria Plebeius Plebeius Plebeius Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Species phyllis phyllis phyllis firdussii firdussii thersites icarus icarus argus argus daphnis daphnis daphnis daphnis klausschuriani klausschuriani klausschuriani klausschuriani klausschuriani klausschuriani klausschuriani birunii birunii birunii iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon iphidamon alcedo marcida marcida marcida marcida marcida marcida marcida marcida marcida marcida marcida bellargus thersites icarus phyllis phyllis phyllis phyllis phyllis phyllis phyllis phyllis elbursicus elbursicus elbursicus elbursicus elbursicus alcedo loewii thersites agestis daphnis daphnis loewii loewii loewii aedon erschoffii iphidamon pseudoxerxes pseudoxerxes pseudoxerxes phyllis phyllis phyllis caeruleus caeruleus caeruleus iphidamon caeruleus caeruleus iphidamon caeruleus caeruleus posthumus caeruleus phyllis posthumus phyllis iphidamon iphidamon phyllis caeruleus phyllis phyllis phyllis iphidamon caeruleus posthumus iphidamon List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix Kar Alc C C C C C C P P P P P P C C C C C C C C C C C P P P P C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C P P P P P P P P P P P P P P P P P P P P P P P P P P P DNA Cytb CO1 ND1 ITS2 Fig. 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I-25 1 1 1 1 1 1 I-26 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I-27 1 I-28 1 I-29 Date 16.07.2000 16.07.2000 16.07.2000 16.07.2000 16.07.2000 16.07.2000 16.07.2000 16.07.2000 16.07.2000 16.07.2000 16.07.2000 16.07.2000 16.07.2000 16.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 18.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 19.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 - 152 - Locality Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Qazayd Dagh Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Veresk Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Asadbar Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Area 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan 25 km O Zanjan südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk südl. Veresk westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa westl. Nesa 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E Altitude 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 2300 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 1800-1950 m 2900 m 2900 m 2900 m 2900 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2900 m 2900 m 2900 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m Province Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Zanjan Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Country Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Appendix 2 Code MW 00360 MW 00361 MW 00362 MW 00363 MW 00364 MW 00365 MW 00366 MW 00367 MW 00368 MW 00369 MW 00370 MW 00371 MW 00372 MW 00373 MW 00374 MW 00375 MW 00376 MW 00377 MW 00378 MW 00379 MW 00380 MW 00381 MW 00382 MW 00383 MW 00384 MW 00385 MW 00386 MW 00387 MW 00388 MW 00389 MW 00390 MW 00391 MW 00392 MW 00393 MW 00394 MW 00395 MW 00396 MW 00397 MW 00398 MW 00399 MW 00400 MW 00401 MW 00402 MW 00403 MW 00404 MW 00405 MW 00406 MW 00407 MW 00408 MW 00409 MW 00410 MW 00411 MW 00412 MW 00413 MW 00414 MW 00415 MW 00416 MW 00417 MW 00418 MW 00419 MW 00420 MW 00421 MW 00422 MW 00423 MW 00424 MW 00425 MW 00426 MW 00427 MW 00428 MW 00429 MW 00430 MW 00431 MW 00432 MW 00433 MW 00434 MW 00435 MW 00436 MW 00437 MW 00438 MW 00439 MW 00440 MW 00441 MW 00442 MW 00443 MW 00444 MW 00445 MW 00446 MW 00447 MW 00448 MW 00449 MW 00450 MW 00451 MW 00452 MW 00453 MW 00454 MW 00455 MW 00456 MW 00457 MW 00458 MW 00459 MW 00460 MW 00461 MW 00462 MW 00463 MW 00464 MW 00465 MW 00466 MW 00467 MW 00468 MW 00469 MW 00470 MW 00471 MW 00472 MW 00473 MW 00474 Sex ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♀ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lycaena Lycaena Lysandra Polyommatus Polyommatus Agrodiaetus Agrodiaetus Polyommatus Species phyllis phyllis iphidamon iphidamon aedon caeruleus caeruleus phyllis iphidamon iphidamon posthumus phyllis iphidamon caeruleus iphidamon iphidamon caeruleus posthumus posthumus posthumus phyllis iphidamon phyllis caeruleus erschoffii erschoffii caeruleus iphidamon iphidamon iphidamon caeruleus caeruleus iphidamon erschoffii erschoffii erschoffii erschoffii erschoffii erschoffii erschoffii caeruleus caeruleus caeruleus posthumus phyllis iphidamon erschoffii caeruleus caeruleus caeruleus caeruleus caeruleus icarus icarus caeruleus iphidamon iphidamon caeruleus caeruleus posthumus iphidamon caeruleus iphidamon iphidamon iphidamon erschoffii caeruleus caeruleus caeruleus caeruleus caeruleus aedon phyllis caeruleus posthumus aedon aedon aedon posthumus phyllis birunii birunii birunii birunii birunii birunii birunii phyllis darius birunii birunii phyllis phyllis phyllis phyllis phyllis phyllis birunii phyllis phyllis phyllis phyllis phyllis birunii phyllis phyllis birunii thetis thetis bellargus icarus icarus birunii birunii aedon List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C Kar Alc P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P DNA Cytb CO1 ND1 ITS2 Fig. 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Date 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 21.07.2000 22.07.2000 22.07.2000 22.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 1 I-30 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 24.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 1 I-31 23.07.2000 23.07.2000 23.07.2000 1 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 24.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 I-32 23.07.2000 23.07.2000 23.07.2000 23.07.2000 23.07.2000 24.07.2000 24.07.2000 24.07.2000 24.07.2000 25.07.2000 25.07.2000 25.07.2000 25.07.2000 25.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 1 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 1 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 26.07.2000 1 26.07.2000 26.07.2000 26.07.2000 27.07.2000 27.07.2000 28.07.2000 - 153 - Locality Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Shakuh Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Shakuh Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Hajiabad Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Shakuh Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Polur Pul-e Zanguleh Pul-e Zanguleh Golestanak Nature Reserve Area 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 36°32.418'N-54°25.956 E 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 36°32.418'N-54°25.956 E 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 25 km SSW Gorgan 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 35 km S Gorgan 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E 36°32.418'N-54°25.956 E südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur südl. Polur 15 km NO Kendevan 15 km NO Kendevan 25 km NO Kendevan Altitude 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 3000 m 3000 m 3000 m 3000 m 2600 m 2600 m 2600 m 2600 m 2600 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2600 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2600 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2150 m 2600 m 2600 m 2600 m 2900 m 2600 m 2600 m 2600 m 2600 m 2600 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2400 m 2400 m 2700-3200 m Province Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Golestan Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Mazandaran Mazandaran Mazandaran Country Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Appendix 2 Code MW 00475 MW 00476 MW 00477 MW 00478 MW 00479 MW 00480 MW 00481 MW 00482 MW 00483 MW 00484 MW 00485 MW 00486 MW 00487 MW 00488 MW 00489 MW 00490 MW 00491 MW 00492 MW 00493 MW 00494 MW 00495 MW 00496 MW 00497 MW 00498 MW 00499 MW 00500 MW 00501 MW 00502 MW 00503 MW 00504 MW 00505 MW 00506 MW 00507 MW 00508 MW 00509 MW 00510 MW 00511 MW 00512 MW 00513 MW 00514 MW 00515 MW 00516 MW 00517 MW 00518 MW 00519 MW 00520 MW 00521 MW 00522 MW 00523 MW 00524 MW 00525 MW 00526 MW 00527 MW 00528 MW 00529 MW 00530 MW 00531 MW 00532 MW 00533 MW 00534 MW 00535 MW 00536 MW 00537 MW 00538 MW 00539 MW 00540 MW 00541 MW 00542 MW 00543 MW 00544 MW 00545 MW 00546 MW 00547 MW 00548 MW 00549 MW 00550 MW 00551 MW 00552 MW 01001 MW 01002 MW 01003 MW 01004 MW 01005 MW 01006 MW 01007 MW 01008 MW 01009 MW 01010 MW 01011 MW 01012 MW 01013 MW 01014 MW 01015 MW 01016 MW 01017 MW 01018 MW 01019 MW 01020 MW 01021 MW 01022 MW 01023 MW 01024 MW 01025 MW 01026 MW 01027 MW 01028 MW 01029 MW 01030 MW 01031 MW 01032 MW 01033 MW 01034 MW 01035 MW 01036 MW 01037 Sex ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♀ ♂ ♀ ♀ ♀ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ Genus Neolysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Lampides Favonius Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Neolysandra Neolysandra Neolysandra Neolysandra Neolysandra Agrodiaetus Neolysandra Neolysandra Neolysandra Neolysandra Neolysandra Vacciniina Polyommatus Vacciniina Vacciniina Vacciniina Vacciniina Vacciniina Vacciniina Neolysandra Neolysandra Cyaniris Cyaniris Vacciniina Vacciniina Vacciniina Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Cyaniris Lycaena Lycaena Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lycaena Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Lysandra Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Polyommatus Polyommatus Polyommatus Lampides Leptotes Aricia Polyommatus Plebeius Satyrium Satyrium Satyrium Satyrium Satyrium Satyrium Gonepteryx Lysandra Lysandra Agrodiaetus Agrodiaetus Species corona birunii iphidamon iphidamon iphidamon birunii birunii birunii birunii birunii birunii birunii birunii birunii birunii eroides eroides eroides eroides birunii birunii boeticus quercus valiabadi elbursicus elbursicus phyllis birunii birunii corona corona corona corona corona birunii corona corona corona corona corona morgianus amandus morgianus morgianus morgianus morgianus morgianus morgianus corona corona semiargus semiargus morgianus morgianus morgianus eroides eroides eroides eroides eroides eroides semiargus thersamon thersamon dizinensis dizinensis dizinensis dizinensis dizinensis dizinensis dizinensis dizinensis birunii birunii posthumus posthumus posthumus thersamon ainsae ainsae ainsae ainsae ainsae ainsae ainsae ainsae escheri escheri bellargus escheri ripartii ripartii ripartii ripartii ripartii coridon dorylas dorylas dorylas boeticus pirithous agestis icarus argus esculi esculi esculi esculi spini spini cleopatra albicans albicans fabressei fabressei List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C Kar Alc DNA Cytb CO1 ND1 ITS2 Fig. Date Locality P 1 28.07.2000 Golestanak Nature Reserve P 1 1 1 28.07.2000 Golestanak Nature Reserve 1 28.07.2000 Golestanak Nature Reserve 1 28.07.2000 Golestanak Nature Reserve 1 28.07.2000 Golestanak Nature Reserve P 1 28.07.2000 Golestanak Nature Reserve 1 28.07.2000 Golestanak Nature Reserve P 1 28.07.2000 Golestanak Nature Reserve P 1 28.07.2000 Golestanak Nature Reserve P 1 28.07.2000 Golestanak Nature Reserve P 1 28.07.2000 Golestanak Nature Reserve P 1 28.07.2000 Golestanak Nature Reserve P 1 28.07.2000 Golestanak Nature Reserve P 1 28.07.2000 Golestanak Nature Reserve P 1 28.07.2000 Golestanak Nature Reserve 1 28.07.2000 Golestanak Nature Reserve 1 28.07.2000 Golestanak Nature Reserve 1 28.07.2000 Golestanak Nature Reserve 1 28.07.2000 Golestanak Nature Reserve 1 28.07.2000 Golestanak Nature Reserve 1 28.07.2000 Golestanak Nature Reserve 1 30.07.2000 5 km S Valiabad 1 1 1 1 30.07.2000 5 km S Valiabad P 1 1 1 1 I-33 30.07.2000 5 km S Valiabad P 1 30.07.2000 Kendevan 1 30.07.2000 Kendevan 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman P 1 31.07.2000 Takht-e Suleyman 1 1 1 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 1 1 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 31.07.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman P 1 01.08.2000 Takht-e Suleyman 1 1 1 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman 1 1 1 1 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman P 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman 1 01.08.2000 Takht-e Suleyman P 1 1 1 1 I-34 04.08.2000 Dizin Pass 1 04.08.2000 Dizin Pass 1 04.08.2000 Dizin Pass 1 04.08.2000 Dizin Pass 1 04.08.2000 Dizin Pass 1 04.08.2000 Dizin Pass P 1 04.08.2000 Dizin Pass 1 04.08.2000 Dizin Pass P 1 1 1 1 I-35 04.08.2000 Dizin Pass P 1 04.08.2000 Dizin Pass 1 04.08.2000 Dizin Pass 1 04.08.2000 Dizin Pass 1 04.08.2000 Dizin Pass 1 28.07.2000 Golestanak Nature Reserve P 1 1 1 1 17.07.2001 Ilarduya 1 17.07.2001 Ilarduya P 1 17.07.2001 Ilarduya P 1 17.07.2001 Ilarduya P 1 17.07.2001 Ilarduya P 1 17.07.2001 Ilarduya 1 17.07.2001 Ilarduya 1 17.07.2001 Ilarduya P 1 1 1 17.07.2001 Ilarduya P 1 17.07.2001 Ilarduya 1 1 1 1 17.07.2001 Ilarduya 1 17.07.2001 Ilarduya 1 18.07.2001 Ubierna P 1 1 1 1 I-36 18.07.2001 Ubierna P 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna P 1 18.07.2001 Ubierna 1 1 1 1 18.07.2001 Ubierna P 1 1 1 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna 1 1 1 18.07.2001 Ubierna 1 1 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna 1 1 1 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna 1 1 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna 1 18.07.2001 Ubierna P 1 1 1 1 19.07.2001 Abejar 1 19.07.2001 Abejar P 1 19.07.2001 Abejar P 1 19.07.2001 Abejar - 154 - Area 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan 25 km NO Kendevan Valiabad Valiabad Valiabad 5 km N Kendevan 5 km N Kendevan südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südwestl. Marzanabad südl. Dizin südl. Dizin südl. Dizin südl. Dizin südl. Dizin südl. Dizin südl. Dizin südl. Dizin südl. Dizin südl. Dizin südl. Dizin südl. Dizin südl. Dizin 25 km NO Kendevan westl. Eguino westl. Eguino westl. Eguino westl. Eguino westl. Eguino westl. Eguino westl. Eguino westl. Eguino westl. Eguino westl. Eguino westl. Eguino westl. Eguino 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos 20 km N Burgos Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Altitude 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 2700-3200 m 1900 m 1900 m 1900 m 2150 m 2150 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3000 m 3600 m 3600 m 3600 m 3600 m 3600 m 3600 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3500-3700 m 3200-3300 m 3200-3300 m 3200-3300 m 3200-3300 m 3200-3300 m 3200-3300 m 3200-3300 m 3200-3300 m 3200-3300 m 3200-3300 m 3200-3300 m 3200-3300 m 3200-3300 m 2700-3200 m 550 m 550 m 550 m 550 m 550 m 550 m 550 m 550 m 550 m 550 m 550 m 550 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 1100 m 1100 m 1100 m 1100 m Province Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Mazandaran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Tehran Mazandaran Alava Alava Alava Alava Alava Alava Alava Alava Alava Alava Alava Alava Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Burgos Soria Soria Soria Soria Country Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Appendix 2 Code MW 01038 MW 01039 MW 01040 MW 01041 MW 01042 MW 01043 MW 01044 MW 01045 MW 01046 MW 01047 MW 01048 MW 01049 MW 01050 MW 01051 MW 01052 MW 01053 MW 01054 MW 01055 MW 01056 MW 01057 MW 01058 MW 01059 MW 01060 MW 01061 MW 01062 MW 01063 MW 01064 MW 01065 MW 01066 MW 01067 MW 01068 MW 01069 MW 01070 MW 01071 MW 01072 MW 01073 MW 01074 MW 01075 MW 01076 MW 01077 MW 01078 MW 01079 MW 01080 MW 01081 MW 01082 MW 01083 MW 01084 MW 01085 MW 01086 MW 01087 MW 01088 MW 01089 MW 01090 MW 01091 MW 01092 MW 01093 MW 01094 MW 01095 MW 01096 MW 01097 MW 01098 MW 01099 MW 01100 MW 01101 MW 01102 MW 01103 MW 01104 MW 01105 MW 01106 MW 01107 MW 01108 MW 01109 MW 01110 MW 01111 MW 01112 MW 01113 MW 01114 MW 01115 MW 01116 MW 01117 MW 01118 MW 01119 MW 01120 MW 01121 MW 01401 MW 01402 MW 01403 MW 01404 MW 01405 MW 01406 MW 01407 MW 01408 MW 01409 MW 01410 MW 01411 MW 01412 MW 01413 MW 01414 MW 01415 MW 01416 MW 02001 MW 02002 MW 02003 MW 02004 MW 02005 MW 02006 MW 02007 MW 02008 MW 02009 MW 02010 MW 02011 MW 02012 MW 02013 MW 02014 MW 02015 Sex ♂ ♂ ♂ ♀ ♀ ♀ ♀ ♀ ♀ ♀ ♂ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♀ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♀ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Aricia Polyommatus Lycaeides Satyrium Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Polyommatus Aricia Leptidea Coenonympha Pyronia Pyronia Pyronia Maniola Hipparchia Hipparchia Hipparchia Hipparchia Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Lysandra Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Coenonympha Coenonympha Lasiommata Melanargia Lysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Leptotes Agrodiaetus Lysandra Lysandra Papilio Lysandra Lysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lasiommata Iphiclides Lysandra Lysandra Arethusana Pararge Pararge Cacyreus Minois Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Zygaena Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Lycaena Celastrina Lycaena Pararge Hyponephele Gonepteryx Argynnis Aricia Pseudochazara Species fabressei fabressei fabressei fabressei fabressei fabressei fabressei fabressei fabressei fabressei agestis icarus idas acaciae ainsae ainsae ainsae ainsae ainsae ainsae ainsae albicans thersites cramera sinapis dorus cecilia tithonus bathseba jurtina semele fagi fidia fidia ripartii ripartii ripartii ainsae ainsae ainsae ainsae ainsae albicans albicans escheri thersites thersites thersites thersites thersites arcania dorus megera galathea albicans ainsae ainsae ainsae ainsae pirithous ainsae albicans albicans machaon coridon coridon ripartii ripartii fulgens fulgens fulgens fulgens fulgens fulgens fulgens megera feisthamelii coridon coridon arethusa aegeria aegeria marshalli dryas filipendulae filipendulae lonicerae lonicerae lonicerae lonicerae lonicerae filipendulae lonicerae hilaris occitanica occitanica fausta occitanica filipendulae filipendulae amandus amandus amandus amandus amandus icarus alciphron argiolus phlaeas aegeria maroccana cleopatra pandora artaxerxes atlantis List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C Kar Alc DNA Cytb CO1 ND1 ITS2 Fig. Date Locality P 1 19.07.2001 Abejar P 1 1 1 1 19.07.2001 Abejar P 1 19.07.2001 Abejar 1 19.07.2001 Abejar 1 19.07.2001 Abejar 1 19.07.2001 Abejar 1 19.07.2001 Abejar 1 19.07.2001 Abejar 1 19.07.2001 Abejar 1 19.07.2001 Abejar 1 1 1 1 19.07.2001 Abejar 1 19.07.2001 Abejar 1 19.07.2001 Abejar 1 19.07.2001 Montenegro de Camaros P 1 20.07.2001 Sta. Maria P 1 1 1 1 I-37 20.07.2001 Sta. Maria P 1 20.07.2001 Sta. Maria P 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria P 1 1 1 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 1 1 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria 1 20.07.2001 Sta. Maria P 1 1 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste P 1 21.07.2001 Triste P 1 1 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 1 1 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 1 1 1 22.07.2001 Boltana 1 22.07.2001 Boltana 1 22.07.2001 Boltana 1 22.07.2001 Boltana 1 22.07.2001 Boltana 1 22.07.2001 Boltana 1 23.07.2001 Boltana 1 23.07.2001 Boltana 1 23.07.2001 Boltana 1 23.07.2001 Boltana 1 23.07.2001 Sta. Coloma de Queralt 1 1 1 23.07.2001 Sta. Coloma de Queralt P 1 23.07.2001 Sta. Coloma de Queralt P 1 1 1 23.07.2001 Sta. Coloma de Queralt P 1 23.07.2001 Sta. Coloma de Queralt P 1 1 1 1 I-38 23.07.2001 Sta. Coloma de Queralt P 1 23.07.2001 Sta. Coloma de Queralt P 1 23.07.2001 Sta. Coloma de Queralt P 1 23.07.2001 Sta. Coloma de Queralt 1 23.07.2001 Sta. Coloma de Queralt 1 23.07.2001 Sta. Coloma de Queralt 1 23.07.2001 Sta. Coloma de Queralt 1 1 1 24.07.2001 Taradell 1 24.07.2001 Taradell 1 1 1 1 24.07.2001 Taradell 1 24.07.2001 Seva 1 25.07.2001 Lloret de Mar 1 25.07.2001 Lloret de Mar 1 1 1 27.07.2001 Maruéjols-les-Gardons 1 27.07.2001 Maruéjols-les-Gardons 1 19.07.2001 Abejar 1 19.07.2001 Abejar 1 19.07.2001 Montenegro de Camaros 1 19.07.2001 Montenegro de Camaros 1 19.07.2001 Montenegro de Camaros 1 19.07.2001 Montenegro de Camaros 1 19.07.2001 Montenegro de Camaros 1 19.07.2001 Montenegro de Camaros 1 19.07.2001 Montenegro de Camaros 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 21.07.2001 Triste 1 24.07.2001 Taradell 1 24.07.2001 Taradell 1 1 1 1 09.07.2002 Oukaimeden 1 09.07.2002 Oukaimeden 1 09.07.2002 Oukaimeden 1 09.07.2002 Oukaimeden 1 09.07.2002 Oukaimeden 1 1 1 1 09.07.2002 Oukaimeden 1 1 1 09.07.2002 Oukaimeden 1 1 1 09.07.2002 Oukaimeden 1 1 1 09.07.2002 Oukaimeden 1 09.07.2002 Oukaimeden 1 09.07.2002 Oukaimeden 1 09.07.2002 Oukaimeden 1 09.07.2002 Oukaimeden 1 09.07.2002 Oukaimeden 1 09.07.2002 Oukaimeden - 155 - Area Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Cabrejas Sierra de Urbión Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Embalse de la Pena Pirineos Centrals Pirineos Centrals Pirineos Centrals Pirineos Centrals Pirineos Centrals Pirineos Centrals Pirineos Centrals Pirineos Centrals Pirineos Centrals Pirineos Centrals SO Vic SO Vic SO Vic S Vic Sierra de Cabrejas Sierra de Cabrejas Sierra de Urbión Sierra de Urbión Sierra de Urbión Sierra de Urbión Sierra de Urbión Sierra de Urbión Sierra de Urbión Sierra de Urbión Sierra de Urbión Sierra de Urbión Sierra de Urbión Sierra de Urbión SO Vic SO Vic Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Altitude 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1200 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 500 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 650 m 650 m 650 m 650 m 650 m 650 m 650 m 650 m 650 m 650 m 700 m 700 m 700 m 700 m 700 m 700 m 700 m 700 m 700 m 700 m 700 m 700 m 700 m 700 m 700 m 600 m 600 m 600 m 100 m 100 m 1100 m 1100 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 600 m 600 m 600 m 600 m 600 m 600 m 600 m 2300m 2300m 2300m 2300m 2300m 2300m 2300m 2300m 2300m 2300m 2300m 2300m 2300m 2400m 2800m Province Soria Soria Soria Soria Soria Soria Soria Soria Soria Soria Soria Soria Soria Soria Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Huesca Tarragona Tarragona Tarragona Tarragona Tarragona Tarragona Tarragona Tarragona Tarragona Tarragona Tarragona Tarragona Barcelona Barcelona Barcelona Barcelona Girona Girona Hérault Hérault Soria Soria Soria Soria Soria Soria Soria Soria Soria Soria Soria Soria Soria Soria Barcelona Barcelona Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Country Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain France France Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Spain Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Appendix 2 Code MW 02016 MW 02017 MW 02018 MW 02019 MW 02020 MW 02021 MW 02022 MW 02023 MW 02024 MW 02025 MW 02026 MW 02027 MW 02028 MW 02029 MW 02030 MW 02031 MW 02032 MW 02033 MW 02034 MW 02035 MW 02036 MW 02037 MW 02038 MW 02039 MW 02040 MW 02041 MW 02042 MW 98001 MW 98002 MW 98003 MW 98004 MW 98005 MW 98006 MW 98007 MW 98008 MW 98009 MW 98010 MW 98011 MW 98012 MW 98013 MW 98014 MW 98015 MW 98016 MW 98017 MW 98018 MW 98019 MW 98020 MW 98021 MW 98022 MW 98023 MW 98024 MW 98025 MW 98026 MW 98027 MW 98028 MW 98029 MW 98030 MW 98031 MW 98032 MW 98033 MW 98034 MW 98035 MW 98036 MW 98037 MW 98038 MW 98039 MW 98040 MW 98041 MW 98042 MW 98043 MW 98044 MW 98045 MW 98046 MW 98047 MW 98048 MW 98049 MW 98050 MW 98051 MW 98052 MW 98053 MW 98054 MW 98055 MW 98056 MW 98057 MW 98058 MW 98059 MW 98060 MW 98061 MW 98062 MW 98063 MW 98064 MW 98065 MW 98066 MW 98067 MW 98068 MW 98069 MW 98070 MW 98071 MW 98072 MW 98073 MW 98074 MW 98075 MW 98076 MW 98077 MW 98078 MW 98079 MW 98080 MW 98081 MW 98082 MW 98083 MW 98084 MW 98085 MW 98086 MW 98087 MW 98088 Sex ♂ ♂ ♀ ♂ ♀ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♀ ♀ ♀ ♀ ♀ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♀ ♂ ♀ ♂ Genus Pseudochazara Coenonympha Pieris Pontia Hipparchia Zizeeria Berberia Berberia Iphiclides Tarucus Tarucus Polyommatus Lampides Hyponephele Hyponephele Pseudophilotes Pseudophilotes Aricia Cyaniris Polyommatus Carcharodus Satyrus Issoria Gonepteryx Colias Thymelicus Pieris Polyommatus Cupido Cupido Plebeius Plebeius Lycaena Polyommatus Polyommatus Agrodiaetus Agrodiaetus Meleageria Meleageria Meleageria Satyrium Plebeius Plebeius Aricia Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Meleageria Agrodiaetus Meleageria Meleageria Plebeius Lycaena Lycaena Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Species atlantis vaucheri segonzaki daplidice aristaeus knysna lambessanus lambessanus feisthamelii theophrastus theophrastus icarus boeticus maroccana maroccana abencerragus abencerragus artaxerxes semiargus icarus tripolinus ferula lathonia cleopatra crocea acteon brassicae icarus osiris osiris loewii loewii thersamon thersites thersites carmon carmon daphnis daphnis daphnis ilicis loewii loewii agestis icarus menalcas menalcas menalcas menalcas menalcas menalcas menalcas admetus admetus daphnis daphnis daphnis daphnis daphnis daphnis daphnis daphnis daphnis daphnis daphnis daphnis daphnis daphnis menalcas menalcas menalcas menalcas menalcas menalcas daphnis iphigenia daphnis daphnis loewii alciphron alciphron admetus admetus admetus admetus admetus admetus admetus admetus admetus menalcas menalcas menalcas menalcas menalcas lycius lycius lycius lycius lycius lycius lycius lycius lycius lycius lycius menalcas menalcas admetus admetus admetus admetus admetus admetus admetus List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C Kar Alc P P P P P P P P P P P P C P C C C P C C C C P P P P C C C P DNA Cytb CO1 ND1 ITS2 Fig. 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Date 09.07.2002 12.07.2002 12.07.2002 12.07.2002 12.07.2002 11.07.2002 12.07.2002 12.07.2002 11.07.2002 1 14.07.2002 14.07.2002 14.07.2002 14.07.2002 14.07.2002 14.07.2002 15.07.2002 15.07.2002 1 15.07.2002 1 15.07.2002 15.07.2002 15.07.2002 15.07.2002 15.07.2002 15.07.2002 15.07.2002 15.07.2002 15.07.2002 11.07.1998 11.07.1998 11.07.1998 11.07.1998 11.07.1998 11.07.1998 11.07.1998 11.07.1998 1 I-39 11.07.1998 11.07.1998 11.07.1998 11.07.1998 11.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 1 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 1 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 12.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 13.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 1 I-40 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 15.07.1998 - 156 - Locality Oukaimeden Tizi-n-Tacheddirt Tizi-n-Tacheddirt Tizi-n-Tacheddirt Tizi-n-Tacheddirt 10 km N Tizi-n-Test Tizi-n-Tacheddirt Tizi-n-Tacheddirt 10 km N Tizi-n-Test Tourchte Tourchte Tourchte Tourchte Tourchte Tourchte Oukaimeden Oukaimeden Oukaimeden Oukaimeden Oukaimeden Oukaimeden Oukaimeden Oukaimeden Oukaimeden Oukaimeden Oukaimeden Oukaimeden Karabayir Karabayir Karabayir Karabayir Karabayir Karabayir Karabayir Karabayir Karabayir Karabayir Karabayir Karabayir Karabayir Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Gülübeli Geçidi Salur Dagi Salur Dagi Salur Dagi Salur Dagi Salur Dagi Salur Dagi Salur Dagi Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Camkuyuzu Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Cukurelma Area Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas Hoher Atlas südl. Korkuteli südl. Korkuteli südl. Korkuteli südl. Korkuteli südl. Korkuteli südl. Korkuteli südl. Korkuteli südl. Korkuteli südl. Korkuteli südl. Korkuteli südl. Korkuteli südl. Korkuteli südl. Korkuteli westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali westl. Elmali Bey Daglari Bey Daglari Bey Daglari Bey Daglari Bey Daglari Bey Daglari Bey Daglari Bey Daglari Bey Daglari Bey Daglari Bey Daglari Bey Daglari Bey Daglari Bey Daglari nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali nördl. Elmali Altitude 2800m 3000m 3200m 3200m 3200m 1700m 3000m 3000m 1700m 1400m 1400m 1400m 1400m 1400m 1400m 2700m 2700m 2700m 2700m 2700m 2700m 2700m 2700m 2700m 2700m 2700m 2700m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1700-1900 m 1700-1900 m 1700-1900 m 1700-1900 m 1700-1900 m 1700-1900 m 1700-1900 m 1750 m 1750 m 1750 m 1750 m 1750 m 1750 m 1750 m 1750 m 1750 m 1750 m 1750 m 1750 m 1750 m 1750 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m Province Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Marrakech Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Fethiye Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Antalya Country Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Morocco Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Appendix 2 Code MW 98089 MW 98090 MW 98091 MW 98092 MW 98093 MW 98094 MW 98095 MW 98096 MW 98097 MW 98098 MW 98099 MW 98100 MW 98101 MW 98102 MW 98103 MW 98104 MW 98105 MW 98106 MW 98107 MW 98108 MW 98109 MW 98110 MW 98111 MW 98112 MW 98113 MW 98114 MW 98115 MW 98116 MW 98117 MW 98118 MW 98119 MW 98120 MW 98121 MW 98122 MW 98123 MW 98124 MW 98125 MW 98126 MW 98127 MW 98128 MW 98129 MW 98130 MW 98131 MW 98132 MW 98133 MW 98134 MW 98135 MW 98136 MW 98137 MW 98138 MW 98139 MW 98140 MW 98141 MW 98142 MW 98143 MW 98144 MW 98145 MW 98146 MW 98147 MW 98148 MW 98149 MW 98150 MW 98151 MW 98152 MW 98153 MW 98154 MW 98155 MW 98156 MW 98157 MW 98158 MW 98159 MW 98160 MW 98161 MW 98162 MW 98163 MW 98164 MW 98165 MW 98166 MW 98167 MW 98168 MW 98169 MW 98170 MW 98171 MW 98172 MW 98173 MW 98174 MW 98175 MW 98176 MW 98177 MW 98178 MW 98179 MW 98180 MW 98181 MW 98182 MW 98183 MW 98184 MW 98185 MW 98186 MW 98187 MW 98188 MW 98189 MW 98190 MW 98191 MW 98192 MW 98193 MW 98194 MW 98195 MW 98196 MW 98197 MW 98198 MW 98199 MW 98200 MW 98201 MW 98202 MW 98203 Sex ♂ ♂ ♀ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Plebeius Lycaena Lycaena Lycaena Agrodiaetus Agrodiaetus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lycaena Lycaena Lycaena Lycaena Polyommatus Polyommatus Lysandra Lysandra Meleageria Meleageria Polyommatus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Pseudophilotes Lysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Species lycius lycius ripartii alcestis argus thersamon thersamon thetis ernesti iphicarmon dorylas ripartii iphicarmon iphicarmon iphicarmon iphicarmon iphicarmon iphigenia iphicarmon ripartii ripartii ripartii ripartii ripartii iphicarmon iphicarmon iphicarmon iphicarmon iphicarmon iphicarmon iphicarmon ripartii iphicarmon ernesti thetis virgaureae virgaureae phlaeas dorylas dorylas ossmar ossmar daphnis daphnis icarus icarus icarus wagneri poseidon poseidon wagneri poseidon poseidon poseidon poseidon poseidon poseidon poseidon poseidon poseidon wagneri poseidon wagneri poseidon poseidon poseidon ossmar iphigenia iphigenia actis actis actis poseidon actis actis ripartii actis actis poseidon actis poseidon maraschi actis menalcas actis maraschi poseidon actis menalcas poseidon poseidon poseidon poseidon poseidon poseidon actis ossmar vicrama ossmar hopfferi hopfferi hopfferi hopfferi hopfferi hopfferi hopfferi hopfferi hopfferi hopfferi hopfferi hopfferi hopfferi hopfferi hopfferi mithridates List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C Kar Alc DNA Cytb CO1 ND1 ITS2 Fig. Date Locality P 1 1 1 20.07.1998 Güneykent P 1 20.07.1998 Güneykent 1 20.07.1998 Güneykent 1 20.07.1998 Güneykent 1 20.07.1998 Güneykent 1 1 1 20.07.1998 Güneykent 1 20.07.1998 Güneykent 1 20.07.1998 Güneykent P 1 1 1 1 I-41 21.07.1998 Dedegöl Geçidi P 1 21.07.1998 Dedegöl Geçidi P 1 21.07.1998 Dedegöl Geçidi P 1 1 21.07.1998 Dedegöl Geçidi P 1 21.07.1998 Dedegöl Geçidi P 1 1 1 21.07.1998 Dedegöl Geçidi P 1 1 1 1 I-42 21.07.1998 Dedegöl Geçidi P 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi P 1 1 1 21.07.1998 Dedegöl Geçidi P 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi P 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 1 1 21.07.1998 Dedegöl Geçidi 1 1 1 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 1 1 21.07.1998 Dedegöl Geçidi 1 21.07.1998 Dedegöl Geçidi 1 1 21.07.1998 Dedegöl Geçidi P 1 1 1 1 I-43 22.07.1998 Zelve P 1 1 22.07.1998 Zelve P 1 1 1 1 22.07.1998 Zelve P 1 1 1 1 22.07.1998 Zelve P 1 22.07.1998 Zelve P 1 22.07.1998 Zelve P 1 22.07.1998 Zelve P 1 22.07.1998 Zelve P 1 22.07.1998 Zelve P 1 22.07.1998 Zelve 1 22.07.1998 Zelve 1 22.07.1998 Zelve 1 22.07.1998 Zelve P 1 1 22.07.1998 Zelve 1 22.07.1998 Zelve 1 22.07.1998 Zelve 1 22.07.1998 Zelve 1 22.07.1998 Zelve P 1 1 1 1 1 I-44 22.07.1998 Zelve P 1 22.07.1998 Zelve 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar P 1 25.07.1998 Gökpinar P 1 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar P 1 1 1 1 I-45 25.07.1998 Gökpinar 1 1 25.07.1998 Gökpinar P 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar P 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar P 1 1 1 1 I-46 25.07.1998 Gökpinar P 1 25.07.1998 Gökpinar 1 1 1 1 1 I-47 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar P 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar P 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar P 1 1 1 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar 1 1 25.07.1998 Gökpinar P 1 1 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar 1 1 1 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar 1 25.07.1998 Gökpinar 1 1 27.07.1998 Gündüzbey P 1 1 1 1 I-48 27.07.1998 Gündüzbey P 1 27.07.1998 Gündüzbey P 1 27.07.1998 Gündüzbey 1 27.07.1998 Gündüzbey 1 27.07.1998 Gündüzbey 1 27.07.1998 Gündüzbey P 1 27.07.1998 Gündüzbey 1 27.07.1998 Gündüzbey 1 27.07.1998 Gündüzbey 1 27.07.1998 Gündüzbey 1 27.07.1998 Gündüzbey 1 27.07.1998 Gündüzbey 1 27.07.1998 Gündüzbey 1 1 27.07.1998 Gündüzbey P 1 1 1 1 I-49 27.07.1998 Gündüzbey - 157 - Area Barla Dagi Barla Dagi Barla Dagi Barla Dagi Barla Dagi Barla Dagi Barla Dagi Barla Dagi westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova westl. Kurucuova nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp nördl. Ürgüp Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Gürün Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Altitude 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1100 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1700 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m Province Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Isparta Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Nevsehir Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Sivas Malatya Malatya Malatya Malatya Malatya Malatya Malatya Malatya Malatya Malatya Malatya Malatya Malatya Malatya Malatya Malatya Country Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Appendix 2 Code MW 98204 MW 98205 MW 98206 MW 98207 MW 98208 MW 98209 MW 98210 MW 98211 MW 98212 MW 98213 MW 98214 MW 98215 MW 98216 MW 98217 MW 98218 MW 98219 MW 98220 MW 98221 MW 98222 MW 98223 MW 98224 MW 98225 MW 98226 MW 98227 MW 98228 MW 98229 MW 98230 MW 98231 MW 98232 MW 98233 MW 98234 MW 98235 MW 98236 MW 98237 MW 98238 MW 98239 MW 98240 MW 98241 MW 98242 MW 98243 MW 98244 MW 98245 MW 98246 MW 98247 MW 98248 MW 98249 MW 98250 MW 98251 MW 98252 MW 98253 MW 98254 MW 98255 MW 98256 MW 98257 MW 98258 MW 98259 MW 98260 MW 98261 MW 98262 MW 98263 MW 98264 MW 98265 MW 98266 MW 98267 MW 98268 MW 98269 MW 98270 MW 98271 MW 98272 MW 98273 MW 98274 MW 98275 MW 98276 MW 98277 MW 98278 MW 98279 MW 98280 MW 98281 MW 98282 MW 98283 MW 98284 MW 98285 MW 98286 MW 98287 MW 98288 MW 98289 MW 98290 MW 98291 MW 98292 MW 98293 MW 98294 MW 98295 MW 98296 MW 98297 MW 98298 MW 98299 MW 98300 MW 98301 MW 98302 MW 98303 MW 98304 MW 98305 MW 98306 MW 98307 MW 98308 MW 98309 MW 98310 MW 98311 MW 98312 MW 98313 MW 98314 MW 98315 MW 98316 MW 98317 MW 98318 Sex ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♀ ♂ ♀ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Meleageria Lysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Plebeius Plebeius Plebeius Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Lysandra Lycaena Lycaena Lycaena Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Polyommatus Lysandra Celastrina Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Plebeius Plebeius Polyommatus Meleageria Meleageria Meleageria Meleageria Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Lampides Agrodiaetus Agrodiaetus Agrodiaetus Aricia Aricia Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Meleageria Lysandra Agrodiaetus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Agrodiaetus Agrodiaetus Lysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Meleageria Meleageria Species hopfferi dama hopfferi daphnis bellargus hopfferi admetus admetus alcestis alcestis alcestis ripartii alcestis alcestis alcestis loewii loewii loewii cornelia cornelia cornelia cornelia cornelia thersites syriaca tityrus tityrus tityrus alcestis alcestis firdussii cornelia cornelia cornelia syriaca argiolus theresiae theresiae theresiae theresiae alcestis admetus syriaca thersites icarus thersites icarus thersites loewii loewii cornelia daphnis daphnis daphnis daphnis iphigenia firdussii schuriani firdussii ripartii cornelia boeticus iphigenia iphigenia iphigenia isauricus anteros daphnis daphnis daphnis daphnis daphnis daphnis daphnis bellargus sigberti icarus icarus sigberti sigberti sigberti sigberti sigberti sigberti sigberti iphigenia sigberti sigberti sigberti guezelmavi guezelmavi guezelmavi guezelmavi guezelmavi guezelmavi guezelmavi thersites guezelmavi ripartii bellargus iphigenia sertavulensis sertavulensis sertavulensis sertavulensis sertavulensis sertavulensis iphigenia iphigenia sertavulensis sertavulensis alcestis alcestis daphnis daphnis List of m at erial Wgs Fix 1C 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 C C C C C C C C C C C C C Kar Alc P P P C P C P C C C C C C C P C C C C C C C C P P P P P P P C C P C C C C C C P P C C C C C C C C C C C C C C C C C P P P P P P C C C C C C C C C C P P P P P P P C C C C C C C C C C C C P P P P P P P P DNA Cytb CO1 ND1 ITS2 Fig. 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I-50 1 1 1 1 1 1 1 1 1 1 1 1 1 1 II-1 1 1- 1 1 1 1 1 1 1 1 1 1 1 1 1 II-2 II-3 1 1 1 1 II-4 1 1 1 1 1 1 1 II-5 Date 27.07.1998 27.07.1998 27.07.1998 27.07.1998 27.07.1998 27.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 28.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 29.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 30.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 31.07.1998 04.08.1998 04.08.1998 04.08.1998 04.08.1998 04.08.1998 04.08.1998 04.08.1998 04.08.1998 05.08.1998 05.08.1998 05.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 06.08.1998 - 158 - Locality Gündüzbey Gündüzbey Gündüzbey Gündüzbey Gündüzbey Gündüzbey Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Saimbeyli-Fälle Gezbeli Geçidi Gezbeli Geçidi Gezbeli Geçidi Gezbeli Geçidi Gezbeli Geçidi Gezbeli Geçidi Gezbeli Geçidi Erciyes Dagi Erciyes Dagi Erciyes Dagi Erciyes Dagi Erciyes Dagi Erciyes Dagi Erciyes Dagi Erciyes Dagi Erciyes Dagi Erciyes Dagi Erciyes Dagi Erciyes Dagi Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Ala Daglar Taskent Taskent Taskent Taskent Taskent Taskent Taskent Taskent Taskent Taskent Taskent Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Yellibeli Geçidi Area Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Yesilyurt Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Saimbeyli Kayakevi Kayakevi Kayakevi Kayakevi Kayakevi Kayakevi Kayakevi Kayakevi Kayakevi Kayakevi Kayakevi Kayakevi Yahyali Yahyali Yahyali Yahyali Yahyali Yahyali Yahyali Yahyali Yahyali Yahyali Yahyali Yahyali Yahyali Yahyali Yahyali Ermenek Ermenek Ermenek Ermenek Ermenek Ermenek Ermenek Ermenek Ermenek Ermenek Ermenek Ermenek Ermenek Ermenek Ermenek Altitude 1300 m 1300 m 1300 m 1300 m 1300 m 1300 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1200-1500 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 1600 m 2900 m 2900 m 2900 m 2700 m 2700 m 2700 m 2700 m 2700 m 2700 m 2700 m 2700 m 2700 m 2700 m 2700 m 1450 m 1450 m 1450 m 1450 m 1450 m 1450 m 1450 m 1450 m 1450 m 1450 m 1450 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m Province Malatya Malatya Malatya Malatya Malatya Malatya Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Adana Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Kayseri Konya Konya Konya Konya Konya Konya Konya Konya Konya Konya Konya Karaman Karaman Karaman Karaman Karaman Karaman Karaman Karaman Karaman Karaman Karaman Karaman Karaman Karaman Karaman Country Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Appendix 2 Code MW 98319 MW 98320 MW 98321 MW 98322 MW 99001 MW 99002 MW 99003 MW 99004 MW 99005 MW 99006 MW 99007 MW 99008 MW 99009 MW 99010 MW 99011 MW 99012 MW 99013 MW 99014 MW 99015 MW 99016 MW 99017 MW 99018 MW 99019 MW 99020 MW 99021 MW 99022 MW 99023 MW 99024 MW 99025 MW 99026 MW 99027 MW 99028 MW 99029 MW 99030 MW 99031 MW 99032 MW 99033 MW 99034 MW 99035 MW 99036 MW 99037 MW 99038 MW 99039 MW 99040 MW 99041 MW 99042 MW 99043 MW 99044 MW 99045 MW 99046 MW 99047 MW 99048 MW 99049 MW 99050 MW 99051 MW 99052 MW 99053 MW 99054 MW 99055 MW 99056 MW 99057 MW 99058 MW 99059 MW 99060 MW 99061 MW 99062 MW 99063 MW 99064 MW 99065 MW 99066 MW 99067 MW 99068 MW 99069 MW 99070 MW 99071 MW 99072 MW 99073 MW 99074 MW 99075 MW 99076 MW 99077 MW 99078 MW 99079 MW 99080 MW 99081 MW 99082 MW 99083 MW 99084 MW 99085 MW 99086 MW 99087 MW 99088 MW 99089 MW 99090 MW 99091 MW 99092 MW 99093 MW 99094 MW 99095 MW 99096 MW 99097 MW 99098 MW 99099 MW 99100 MW 99101 MW 99102 MW 99103 MW 99104 MW 99105 MW 99106 MW 99107 MW 99108 MW 99109 MW 99110 MW 99111 Sex ♂ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Polyommatus Satyrium Lycaena Lycaena Aricia Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Neolysandra Neolysandra Polyommatus Agrıades Agrodiaetus Agrıades Agrıades Agrıades Agrıades Agrıades Agrıades Turanana Turanana Plebeius Plebeius Polyommatus Aricia Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Lysandra Polyommatus Polyommatus Lysandra Lysandra Plebeius Maculinea Kretania Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Polyommatus Polyommatus Lysandra Plebeius Meleageria Meleageria Pseudophilotes Pseudophilotes Pseudophilotes Celastrina Celastrina Celastrina Cupido Lycaena Agrodiaetus Agrodiaetus Polyommatus Polyommatus Polyommatus Satyrium Agrodiaetus Agrodiaetus Agrodiaetus Aricia Aricia Aricia Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Species cornelia spini thersamon thersamon torulensis amandus amandus turcicus turcicus firdussii firdussii firdussii iphigenia firdussii firdussii coelestina coelestina dorylas pyrenaicus turcicus pyrenaicus pyrenaicus pyrenaicus pyrenaicus pyrenaicus pyrenaicus endymion endymion loewii loewii icarus agestis icarus icarus hopfferi ripartii ripartii ripartii ripartii admetus admetus cornelia bellargus myrrhinus myrrhinus corydonius corydonius loewii arion eurypilus amandus amandus amandus amandus amandus amandus huberti huberti huberti huberti merhaba artvinensis merhaba carmon putnami iphigenia artvinensis artvinensis artvinensis artvinensis ninae ripartii iphigenia ripartii ripartii thersites icarus icarus icarus corydonius loewii daphnis daphnis vicrama vicrama vicrama argiolus argiolus argiolus osiris alciphron iphigenia iphigenia dorylas eros eros ilicis cyaneus huberti cyaneus isauricus isauricus isauricus cyaneus demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C Kar Alc DNA Cytb CO1 ND1 ITS2 Fig. Date Locality P 1 06.08.1998 Yellibeli Geçidi 1 06.08.1998 Yellibeli Geçidi 1 1 06.08.1998 Yellibeli Geçidi 1 06.08.1998 Yellibeli Geçidi P 1 1 1 1 04.07.1999 Torul 1 04.07.1999 Tersundagi Geç. 1 04.07.1999 Tersundagi Geç. P 1 05.07.1999 Çaglayan P 1 05.07.1999 Çaglayan P 1 1 1 1 II-6 05.07.1999 Çaglayan P 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan P 1 1 1 1 II-7 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan P 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan P 1 1 1 1 05.07.1999 Çaglayan 1 1 1 1 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan P 1 05.07.1999 Çaglayan 1 1 1 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 1 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 05.07.1999 Çaglayan 1 06.07.1999 Çaglayan 1 06.07.1999 Çaglayan P 1 06.07.1999 Çaglayan 1 06.07.1999 Çaglayan 1 06.07.1999 Çaglayan 1 06.07.1999 Çaglayan 1 06.07.1999 Çaglayan 1 1 1 1 06.07.1999 Çaglayan 1 06.07.1999 Çaglayan 1 07.07.1999 Köskköy 1 07.07.1999 Köskköy 1 1 1 07.07.1999 Köskköy 1 07.07.1999 Köskköy 1 07.07.1999 Köskköy 1 1 1 1 07.07.1999 Köskköy 1 07.07.1999 Köskköy 1 1 1 1 07.07.1999 Köskköy 1 07.07.1999 Köskköy 1 07.07.1999 Köskköy 1 07.07.1999 Köskköy 1 07.07.1999 Köskköy 1 07.07.1999 Köskköy P 1 1 1 08.07.1999 Kiliçkaya P 1 08.07.1999 Kiliçkaya P 1 08.07.1999 Kiliçkaya P 1 08.07.1999 Kiliçkaya P 1 1 1 1 II-8 08.07.1999 Kiliçkaya P 1 1 1 1 II-9 08.07.1999 Kiliçkaya P 1 1 1 08.07.1999 Kiliçkaya P 1 1 1 08.07.1999 Kiliçkaya P 1 1 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya P 1 08.07.1999 Kiliçkaya P 1 08.07.1999 Kiliçkaya P 1 08.07.1999 Kiliçkaya P 1 08.07.1999 Kiliçkaya P 1 08.07.1999 Kiliçkaya P 1 1 1 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 1 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 1 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 08.07.1999 Kiliçkaya 1 09.07.1999 Demirkent 1 09.07.1999 Demirkent 1 09.07.1999 Demirkent 1 09.07.1999 Demirkent 1 09.07.1999 Demirkent 1 09.07.1999 Demirkent P 1 1 1 1 11.07.1999 Kagizman P 1 1 1 1 II-10 11.07.1999 Kagizman 1 11.07.1999 Kagizman 1 1 1 1 11.07.1999 Kagizman 1 11.07.1999 Kagizman P 1 11.07.1999 Kagizman P 1 11.07.1999 Kagizman 1 11.07.1999 Akçay P 1 1 1 11.07.1999 Akçay P 1 11.07.1999 Akçay P 1 1 1 11.07.1999 Akçay P 1 1 1 1 1 II-11 11.07.1999 Akçay 1 11.07.1999 Akçay 1 11.07.1999 Akçay 1 11.07.1999 Akçay 1 11.07.1999 Akçay P 1 11.07.1999 Akçay 1 11.07.1999 Akçay - 159 - Area Ermenek Ermenek Ermenek Ermenek 15 km SO Torul Siran Siran 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km O Çaglayan 5 km O Çaglayan 5 km O Çaglayan 5 km O Çaglayan 5 km O Çaglayan 5 km O Çaglayan 5 km O Çaglayan 5 km O Çaglayan 5 km O Çaglayan 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli Yusufeli 20 km O Demirkent 20 km O Demirkent 20 km O Demirkent 20 km O Demirkent 20 km O Demirkent 20 km O Demirkent 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Altitude 1800 m 1800 m 1800 m 1800 m 1100 m 2000 m 2000 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1350 m 1600 m 1600 m 1600 m 1600 m 1600 m 1600 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m Province Karaman Karaman Karaman Karaman Gümüshane Gümüshane Gümüshane Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Artvin Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Country Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Appendix 2 Code MW 99112 MW 99113 MW 99114 MW 99115 MW 99116 MW 99117 MW 99118 MW 99119 MW 99120 MW 99121 MW 99122 MW 99123 MW 99124 MW 99125 MW 99126 MW 99127 MW 99128 MW 99129 MW 99130 MW 99131 MW 99132 MW 99133 MW 99134 MW 99135 MW 99136 MW 99137 MW 99138 MW 99139 MW 99140 MW 99141 MW 99142 MW 99143 MW 99144 MW 99145 MW 99146 MW 99147 MW 99148 MW 99149 MW 99150 MW 99151 MW 99152 MW 99153 MW 99154 MW 99155 MW 99156 MW 99157 MW 99158 MW 99159 MW 99160 MW 99161 MW 99162 MW 99163 MW 99164 MW 99165 MW 99166 MW 99167 MW 99168 MW 99169 MW 99170 MW 99171 MW 99172 MW 99173 MW 99174 MW 99175 MW 99176 MW 99177 MW 99178 MW 99179 MW 99180 MW 99181 MW 99182 MW 99183 MW 99184 MW 99185 MW 99186 MW 99187 MW 99188 MW 99189 MW 99190 MW 99191 MW 99192 MW 99193 MW 99194 MW 99195 MW 99196 MW 99197 MW 99198 MW 99199 MW 99200 MW 99201 MW 99202 MW 99203 MW 99204 MW 99205 MW 99206 MW 99207 MW 99208 MW 99209 MW 99210 MW 99211 MW 99212 MW 99213 MW 99214 MW 99215 MW 99216 MW 99217 MW 99218 MW 99219 MW 99220 MW 99221 MW 99222 MW 99223 MW 99224 MW 99225 MW 99226 Sex ♂ ♂ ♀ ♀ ♀ ♀ ♀ ♀ ♀ ♀ ♀ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Plebeius Cupido Polyommatus Polyommatus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Aricia Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Lysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Satyrium Satyrium Satyrium Agrodiaetus Agrodiaetus Plebeius Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Turanana Turanana Neolysandra Neolysandra Polyommatus Agrodiaetus Species demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi christophi osiris thersites thersites thersites icarus huberti huberti huberti huberti eumedon turcicus firdussii firdussii baytopi dorylas corydonius demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi demavendi iphigenia iphigenia huberti huberti huberti hyrcanicum hyrcanicum hyrcanicum turcicus turcicus pylaon interjectus altivagans firdussii altivagans damon huberti iphigenia firdussii firdussii huberti phyllis phyllis iphigenia turcicus phyllis huberti phyllis iphigenia damon phyllis turcicus phyllis firdussii phyllis phyllis turcicus turcicus phyllis turcicus phyllis iphigenia phyllis ripartii phyllis phyllis phyllis phyllis phyllis turcicus turcicus firdussii phyllis ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii ripartii endymion endymion coelestina coelestina dorylas zapvadi List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C Kar Alc C C C C P P P C C C C P P C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P DNA Cytb CO1 ND1 ITS2 Fig. 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Date 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 11.07.1999 12.07.1999 12.07.1999 12.07.1999 12.07.1999 1 12.07.1999 1 12.07.1999 12.07.1999 12.07.1999 12.07.1999 12.07.1999 12.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 13.07.1999 14.07.1999 14.07.1999 1 14.07.1999 1 II-12 14.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 1 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 1 II-13 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 1 II-14 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 15.07.1999 1 17.07.1999 - 160 - Locality Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Badilli Badilli Badilli Badilli Badilli Badilli Badilli Badilli Badilli Badilli Badilli Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Akçay Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Köskköy Köskköy Köskköy Çiftlik Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Çaglayan Zernek Brj. Area Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Tuzluca Tuzluca Tuzluca Tuzluca Tuzluca Tuzluca Tuzluca Tuzluca Tuzluca Tuzluca Tuzluca Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman Kagizman 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman 30 km SW Kagizman 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 5 km NO Çiftlik 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan 5 km SO Çaglayan Güzelsu Altitude 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1800-2000 m 1800-2000 m 1800-2000 m 1800-2000 m 1800-2000 m 1800-2000 m 1800-2000 m 1800-2000 m 1800-2000 m 1800-2000 m 1800-2000 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1200 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1400 m 1900 m 1900 m 1900 m 1900 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1900 m Province Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Igdir Igdir Igdir Igdir Igdir Igdir Igdir Igdir Igdir Igdir Igdir Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Kars Erzurum Erzurum Erzurum Erzurum Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Erzincan Van Country Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Appendix 2 Code MW 99227 MW 99228 MW 99229 MW 99230 MW 99231 MW 99232 MW 99233 MW 99234 MW 99235 MW 99236 MW 99237 MW 99238 MW 99239 MW 99240 MW 99241 MW 99242 MW 99243 MW 99244 MW 99245 MW 99246 MW 99247 MW 99248 MW 99249 MW 99250 MW 99251 MW 99252 MW 99253 MW 99254 MW 99255 MW 99256 MW 99257 MW 99258 MW 99259 MW 99260 MW 99261 MW 99262 MW 99263 MW 99264 MW 99265 MW 99266 MW 99267 MW 99268 MW 99269 MW 99270 MW 99271 MW 99272 MW 99273 MW 99274 MW 99275 MW 99276 MW 99277 MW 99278 MW 99279 MW 99280 MW 99281 MW 99282 MW 99283 MW 99284 MW 99285 MW 99286 MW 99287 MW 99288 MW 99289 MW 99290 MW 99291 MW 99292 MW 99293 MW 99294 MW 99295 MW 99296 MW 99297 MW 99298 MW 99299 MW 99300 MW 99301 MW 99302 MW 99303 MW 99304 MW 99305 MW 99306 MW 99307 MW 99308 MW 99309 MW 99310 MW 99311 MW 99312 MW 99313 MW 99314 MW 99315 MW 99316 MW 99317 MW 99318 MW 99319 MW 99320 MW 99321 MW 99322 MW 99323 MW 99324 MW 99325 MW 99326 MW 99327 MW 99328 MW 99329 MW 99330 MW 99331 MW 99332 MW 99333 MW 99334 MW 99335 MW 99336 MW 99337 MW 99338 MW 99339 MW 99340 MW 99341 Sex ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Meleageria Meleageria Meleageria Meleageria Kretania Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Vacciniina Neolysandra Turanana Kretania Plebeius Pseudophilotes Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Neolysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Species zapvadi zapvadi zapvadi zapvadi zapvadi cyaneus cyaneus cyaneus turcicola turcicola altivagans firdussii altivagans altivagans firdussii huberti firdussii phyllis phyllis firdussii firdussii phyllis phyllis cyaneus phyllis altivagans altivagans turcicola ? ? icarus turcicola turcicola turcicola turcicola turcicola ripartii ripartii ripartii turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola dantchenkoi dantchenkoi dantchenkoi dantchenkoi dantchenkoi daphnis daphnis daphnis daphnis eurypilus thersites icarus kurdistanicus kurdistanicus kurdistanicus sekercioglui firdussii sekercioglui pierceae baytopi turcicola pierceae pierceae iphigenia alcestis alcestis alcedo fatima endymion eurypilus loewii vicrama iphigenia baytopi zapvadi baytopi iphigenia baytopi baytopi iphigenia turcicola firdussii firdussii turcicola turcicola dantchenkoi dantchenkoi turcicola dantchenkoi fatima turcicola dantchenkoi turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola cyaneus altivagans altivagans altivagans pierceae pierceae pierceae List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C CC C C C C C C C C C C C C C C C C C C C C C C C Kar Alc DNA Cytb CO1 ND1 ITS2 Fig. Date Locality P 1 17.07.1999 Zernek Brj. P 1 17.07.1999 Zernek Brj. P 1 17.07.1999 Zernek Brj. 1 17.07.1999 Zernek Brj. 1 17.07.1999 Zernek Brj. P 1 17.07.1999 Zernek Brj. P 1 17.07.1999 Zernek Brj. P 1 17.07.1999 Zernek Brj. P 1 17.07.1999 Zernek Brj. P 1 17.07.1999 Güzeldere Geç. P 1 17.07.1999 Güzeldere Geç. 1 17.07.1999 Güzeldere Geç. 1 17.07.1999 Güzeldere Geç. P 1 1 1 1 II-15 17.07.1999 Güzeldere Geç. P 1 17.07.1999 Güzeldere Geç. P 1 17.07.1999 Güzeldere Geç. 1 17.07.1999 Güzeldere Geç. 1 1 17.07.1999 Güzeldere Geç. 1 17.07.1999 Güzeldere Geç. 1 17.07.1999 Güzeldere Geç. P 1 1 1 1 17.07.1999 Güzeldere Geç. 1 17.07.1999 Güzeldere Geç. P 1 17.07.1999 Güzeldere Geç. P 1 17.07.1999 Güzeldere Geç. 1 17.07.1999 Güzeldere Geç. P 1 17.07.1999 Güzeldere Geç. P 1 17.07.1999 Güzeldere Geç. P 1 17.07.1999 Güzeldere Geç. 1 17.07.1999 Güzeldere Geç. 1 17.07.1999 Güzeldere Geç. 1 17.07.1999 Güzeldere Geç. P 1 1 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi P 1 1 1 17.07.1999 Kurubas Geçidi P 1 1 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi P 1 1 1 1 II-16 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi P 1 1 1 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi P 1 1 1 1 II-17 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 1 1 II-18 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 1 1 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak 1 18.07.1999 Çatak 1 1 1 1 18.07.1999 Çatak 1 18.07.1999 Çatak 1 1 1 1 18.07.1999 Çatak 1 18.07.1999 Çatak 1 18.07.1999 Çatak 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak 1 1 1 1 II-19 18.07.1999 Çatak 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak 1 1 18.07.1999 Çatak P 1 1 1 18.07.1999 Çatak 1 18.07.1999 Çatak 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 1 1 1 18.07.1999 Çatak P 1 1 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak P 1 18.07.1999 Çatak 1 17.07.1999 Kurubas Geçidi P 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 17.07.1999 Kurubas Geçidi 1 19.07.1999 Kurubas Geçidi P 1 19.07.1999 Kurubas Geçidi 1 19.07.1999 Güzeldere Geç. P 1 19.07.1999 Güzeldere Geç. P 1 19.07.1999 Güzeldere Geç. 1 1 19.07.1999 Güzeldere Geç. 1 19.07.1999 Güzeldere Geç. P 1 1 1 1 II-20 19.07.1999 Güzeldere Geç. - 161 - Area Güzelsu Güzelsu Güzelsu Güzelsu Güzelsu Güzelsu Güzelsu Güzelsu Güzelsu Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak 25-32 km N Çatak Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Gürpinar Baskale Baskale Baskale Baskale Baskale Baskale Altitude 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2000-2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m Province Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Country Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Appendix 2 Code MW 99342 MW 99343 MW 99344 MW 99345 MW 99346 MW 99347 MW 99348 MW 99349 MW 99350 MW 99351 MW 99352 MW 99353 MW 99354 MW 99355 MW 99356 MW 99357 MW 99358 MW 99359 MW 99360 MW 99361 MW 99362 MW 99363 MW 99364 MW 99365 MW 99366 MW 99367 MW 99368 MW 99369 MW 99370 MW 99371 MW 99372 MW 99373 MW 99374 MW 99375 MW 99376 MW 99377 MW 99378 MW 99379 MW 99380 MW 99381 MW 99382 MW 99383 MW 99384 MW 99385 MW 99386 MW 99387 MW 99388 MW 99389 MW 99390 MW 99391 MW 99392 MW 99393 MW 99394 MW 99395 MW 99396 MW 99397 MW 99398 MW 99399 MW 99400 MW 99401 MW 99402 MW 99403 MW 99404 MW 99405 MW 99406 MW 99407 MW 99408 MW 99409 MW 99410 MW 99411 MW 99412 MW 99413 MW 99414 MW 99415 MW 99416 MW 99417 MW 99418 MW 99419 MW 99420 MW 99421 MW 99422 MW 99423 MW 99424 MW 99425 MW 99426 MW 99427 MW 99428 MW 99429 MW 99430 MW 99431 MW 99432 MW 99433 MW 99434 MW 99435 MW 99436 MW 99437 MW 99438 MW 99439 MW 99440 MW 99441 MW 99442 MW 99443 MW 99444 MW 99445 MW 99446 MW 99447 MW 99448 MW 99449 MW 99450 MW 99451 MW 99452 MW 99453 MW 99454 MW 99455 MW 99456 Sex ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♀ ♂ ♀ ♀ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lycaena Lycaena Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lycaena Polyommatus Satyrium Satyrium Satyrium Satyrium Satyrium Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Chilades Chilades Cyaniris Polyommatus Polyommatus Vacciniina Vacciniina Vacciniina Vacciniina Lysandra Lysandra Polyommatus Polyommatus Kretania Lycaena Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Species phyllis phyllis baytopi baytopi pierceae pierceae pierceae iphigenia iphigenia zapvadi turcicola altivagans firdussii pierceae phyllis altivagans pierceae pierceae baytopi baytopi pierceae baytopi baytopi pierceae pierceae baytopi baytopi baytopi pierceae pierceae baytopi zapvadi zapvadi cyaneus antidolus antidolus antidolus antidolus alcestis demavendi demavendi demavendi demavendi demavendi demavendi alciphron tityrus antidolus demavendi demavendi demavendi antidolus antidolus antidolus thersamon icarus myrtale myrtale myrtale myrtale myrtale antidolus antidolus demavendi antidolus demavendi hopfferi hopfferi hopfferi turcicola phyllis firdussii altivagans cyaneus pierceae firdussii pierceae pierceae iphigenia iphigenia firdussii iphigenia bellargus trochylus trochylus semiargus icarus icarus alcedo alcedo alcedo alcedo bellargus bellargus icarus icarus eurypilus phlaeas iphigenia firdussii phyllis firdussii firdussii firdussii bellargus pierceae cyaneus cyaneus turcicola baytopi phyllis hopfferi baytopi baytopi phyllis List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C CCC C C C C C C C C C C C C C C C C C C C C C C C C C C CC C Kar Alc P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P P DNA Cytb CO1 ND1 ITS2 Fig. 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 II-22 1 1 1 1 1 1 1 1 1 1 1 1 II-23 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 II-21 1 1 II-24 1 1 II-25 1 II-26 1 1 1 1 1 1 II-27 Date 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 19.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 20.07.1999 21.07.1999 21.07.1999 21.07.1999 21.07.1999 21.07.1999 21.07.1999 21.07.1999 21.07.1999 21.07.1999 21.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 22.07.1999 23.07.1999 23.07.1999 23.07.1999 23.07.1999 23.07.1999 23.07.1999 23.07.1999 23.07.1999 23.07.1999 23.07.1999 23.07.1999 24.07.1999 24.07.1999 24.07.1999 24.07.1999 24.07.1999 24.07.1999 - 162 - Locality Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Güzeldere Geç. Zernek Brj. Zernek Brj. Zernek Brj. Yüksekova Yüksekova Yüksekova Yüksekova Yüksekova Yüksekova Yüksekova Yüksekova Yüksekova Yüksekova Yüksekova Yüksekova Yüksekova Dilezi Geçidi Dilezi Geçidi Dilezi Geçidi Dilezi Geçidi Haruna Geçidi Haruna Geçidi Haruna Geçidi Haruna Geçidi Haruna Geçidi Haruna Geçidi Haruna Geçidi Haruna Geçidi Haruna Geçidi Haruna Geçidi Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Dez Çay Zernek Brj. Zernek Brj. Zernek Brj. Zernek Brj. Çatak Çatak Çatak Çatak Çatak Çatak Area Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Baskale Güzelsu Güzelsu Güzelsu 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova 22 km NW Yüksekova nordöstl. Yüksekova nordöstl. Yüksekova nordöstl. Yüksekova nordöstl. Yüksekova südöstl. Yüksekova südöstl. Yüksekova südöstl. Yüksekova südöstl. Yüksekova südöstl. Yüksekova südöstl. Yüksekova südöstl. Yüksekova südöstl. Yüksekova südöstl. Yüksekova südöstl. Yüksekova nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari nordöstl. Hakkari Güzelsu Güzelsu Güzelsu Güzelsu 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak Altitude 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2600 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 2500 m 1900 m 1900 m 1900 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 2100 m 2100 m 2100 m 2100 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 2000 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500 m 1500-1700 m 1500-1700 m 1500-1700 m 1500-1700 m 1500-1700 m 1500-1700 m 1500-1700 m 1900 m 1900 m 1900 m 1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m Province Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Hakkari Van Van Van Van Van Van Van Van Van Van Country Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Appendix 2 Code MW 99457 MW 99458 MW 99459 MW 99460 MW 99461 MW 99462 MW 99463 MW 99464 MW 99465 MW 99466 MW 99467 MW 99468 MW 99469 MW 99470 MW 99471 MW 99472 MW 99473 MW 99474 MW 99475 MW 99476 MW 99477 MW 99478 MW 99479 MW 99480 MW 99481 MW 99482 MW 99483 MW 99484 MW 99485 MW 99486 MW 99487 MW 99488 MW 99489 MW 99490 MW 99491 MW 99492 MW 99493 MW 99494 MW 99495 MW 99496 MW 99497 MW 99498 MW 99499 MW 99500 MW 99501 MW 99502 MW 99503 MW 99504 MW 99505 MW 99506 MW 99507 MW 99508 MW 99509 MW 99510 MW 99511 MW 99512 MW 99513 MW 99514 MW 99515 MW 99516 MW 99517 MW 99518 MW 99519 MW 99520 MW 99521 MW 99522 MW 99523 MW 99524 MW 99525 MW 99526 MW 99527 MW 99528 MW 99529 MW 99530 MW 99531 MW 99532 MW 99533 MW 99534 MW 99535 MW 99536 MW 99537 MW 99538 MW 99539 MW 99540 MW 99541 MW 99542 MW 99543 MW 99544 MW 99545 MW 99546 MW 99547 MW 99548 MW 99549 MW 99550 MW 99551 MW 99552 MW 99553 MW 99554 MW 99555 MW 99556 MW 99557 MW 99558 MW 99559 MW 99560 MW 99561 MW 99562 MW 99563 MW 99564 MW 99565 MW 99566 MW 99567 MW 99568 MW 99569 MW 99570 MW 99571 Sex ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Neolysandra Neolysandra Neolysandra Meleageria Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Meleageria Agrodiaetus Lysandra Lycaena Agrodiaetus Agrodiaetus Lysandra Lysandra Lycaena Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Polyommatus Polyommatus Lysandra Polyommatus Polyommatus Lysandra Lysandra Lysandra Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Satyrium Lycaena Plebeius Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Species firdussii turcicus phyllis baytopi baytopi phyllis phyllis turcicola kanduli iphigenia fatima fatima fatima daphnis dantch.Xmenalc. kurdistanicus kurdistanicus kurdistanicus cyaneus zapvadi zapvadi turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola turcicola menalcas menalcas menalcas menalcas menalcas menalcas menalcas putnami huberti huberti putnami putnami putnami putnami ninae ninae ninae ninae daphnis huberti corydonius virgaureae wagneri baytopi corydonius corydonius thetis damon damon damon wagneri wagneri turcicus turcicus turcicus turcicus damon damon menalcas alcestis myrrhinus myrrhinus corydonius myrrhinus myrrhinus corydonius corydonius corydonius icarus icarus alcestis alcestis damon myrtale thetis loewii myrrhinus hopfferi huberti huberti damon firdussii damon huberti huberti tankeri phyllis phyllis huberti tankeri tankeri tankeri tankeri damon damon tankeri tankeri tankeri List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C Kar Alc DNA Cytb CO1 ND1 ITS2 Fig. Date Locality P 1 24.07.1999 Çatak P 1 24.07.1999 Çatak 1 24.07.1999 Çatak 1 24.07.1999 Çatak P 1 24.07.1999 Çatak 1 24.07.1999 Çatak P 1 24.07.1999 Çatak P 1 24.07.1999 Çatak P 1 1 1 1 II-28 24.07.1999 Çatak 1 24.07.1999 Çatak 1 24.07.1999 Çatak 1 24.07.1999 Çatak 1 1 1 24.07.1999 Çatak 1 24.07.1999 Çatak P 1 1 1 1 1 II-29 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi P 1 1 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi P 1 1 1 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi P 1 1 1 1 II-30 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi P 1 1 1 II-31 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi P 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi 1 25.07.1999 Erek Dagi P 1 1 1 1 II-32 26.07.1999 Agrı P 1 26.07.1999 Agrı P 1 26.07.1999 Agrı P 1 26.07.1999 Agrı P 1 26.07.1999 Agrı P 1 26.07.1999 Agrı P 1 26.07.1999 Agrı P 1 1 1 1 II-33 26.07.1999 Agrı P 1 26.07.1999 Agrı P 1 26.07.1999 Agrı P 1 26.07.1999 Agrı 1 26.07.1999 Agrı P 1 26.07.1999 Cumaçay 1 1 1 1 26.07.1999 Gaziler 1 1 1 26.07.1999 Gaziler P 1 27.07.1999 Gaziler P 1 27.07.1999 Gaziler 1 27.07.1999 Gaziler 1 27.07.1999 Gaziler 1 1 1 28.07.1999 Sac Geçidi 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy P 1 28.07.1999 Köskköy P 1 28.07.1999 Köskköy P 1 28.07.1999 Köskköy P 1 1 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy P 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy P 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy P 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy P 1 1 1 1 28.07.1999 Köskköy 1 1 1 1 28.07.1999 Köskköy 1 1 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy 1 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy 1 28.07.1999 Köskköy P 1 28.07.1999 Köskköy P 1 28.07.1999 Köskköy 1 1 1 1 28.07.1999 Köskköy 1 29.07.1999 Kop Geçidi 1 29.07.1999 Kop Geçidi 1 29.07.1999 Kop Geçidi P 1 1 1 1 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi P 1 1 1 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi 1 29.07.1999 Kop Geçidi 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi P 1 1 1 1 29.07.1999 Kop Geçidi 1 29.07.1999 Kop Geçidi 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi P 1 1 1 1 II-34 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi P 1 29.07.1999 Kop Geçidi - 163 - Area 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 25 km N Çatak 25 km N Çatak 25 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak 5-18 km N Çatak Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van 15 km N Agrı 15 km N Agrı 15 km N Agrı 15 km N Agrı 15 km N Agrı 15 km N Agrı 15 km N Agrı 15 km N Agrı 15 km N Agrı 15 km N Agrı 15 km N Agrı 15 km N Agrı 5 km N Cumaçay 10 km N Gaziler 10 km N Gaziler 10 km N Gaziler 10 km N Gaziler 10 km N Gaziler 10 km N Gaziler 20 km W Eleckirt 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum 25 km N Erzurum nördl. Askale nördl. Askale nördl. Askale nördl. Askale nördl. Askale südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden südl. Maden Altitude 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 1600-1900 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 2200 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 2000 m 1800 m 1800 m 1800 m 1800 m 1800 m 1800 m 2000 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 1900 m 2200 m 2200 m 2200 m 2200 m 2200 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m Province Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Van Agrı Agrı Agrı Agrı Agrı Agrı Agrı Agrı Agrı Agrı Agrı Agrı Agrı Igdir Igdir Igdir Igdir Igdir Igdir Agrı Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Erzurum Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Country Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Appendix 2 Code MW 99572 MW 99573 MW 99574 MW 99575 MW 99576 MW 99577 MW 99578 MW 99579 MW 99580 MW 99581 MW 99582 MW 99583 MW 99584 MW 99585 MW 99586 MW 99587 MW 99588 MW 99589 MW 99590 MW 99591 MW 99592 MW 99593 MW 99594 MW 99595 MW 99596 MW 99597 MW 99598 MW 99599 MW 99600 MW 99601 MW 99602 MW 99603 MW 99604 MW 99605 MW 99606 MW 99607 MW 99608 MW 99609 MW 99610 MW 99611 MW 99612 MW 99613 MW 99614 MW 99615 MW 99616 MW 99617 MW 99618 OK 96022 OK 99001 WE 00001 WE 00002 WE 00003 WE 02321 WE 02421 WE 02431 WE 02451 WE 02452 WE 02453 WE 02454 WE 02491 WE 02492 WE 02531 WE 02532 WE 02533 WE 02534 WE 02535 WE 02536 WE 02591 WE 02592 WE 02593 WE 02611 WE 02612 WE 02613 WE 02614 WE 02621 WE 02622 WE 02661 WE 02662 WE 02671 WE 02672 WE 02673 WE 02674 WE 02675 WE 02676 WE 02677 WE 37001 WE 38601 WE 85001 WE 92001 WE 92002 WE 94001 WE 94002 WE 98001 WE 99001 Sum Sex ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♀ ♀ ♀ ♀ ♀ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♀ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ ♂ Genus Agrodiaetus Agrodiaetus Agrodiaetus Plebeius Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Polyommatus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Lysandra Lysandra Lysandra Lysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Lysandra Lysandra Lysandra Agrodiaetus Agrodiaetus Neolysandra Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Agrodiaetus Plebeius Agrodiaetus Agrodiaetus Species phyllis tankeri ripartii pylaon eros eros eros eros eros eros eros eros ninae huberti tankeri phyllis humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae humedasae damon damon bellargus coridon coridon coridon coridon damon damon damon damon damon coridon caelestissimus gennargenti tenhageni glaucias diana pseudoxerxes mofidii khorasanensis tenhageni tenhageni mofidii mofidii achaemenes achaemenes zarathustra zarathustra zarathustra lorestanus lorestanus damalis peilei peilei peilei karindus karindus karindus morgani sennanensis sennanensis arasbarani rovshani femininoides femininoides femininoides gorbunovi gorbunovi hamadanensis demavendi mithridates carmon shahrami phyllides phyllides actinides cyane iphigenides barmifiruze List of m at erial Wgs 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Fix C C C C C C C C C C C C C C C C C C C C C C 1 1 1 1 1 C C C C C C C C C C C 1 1 1 1 1C 1C C 1C 1C C C 1C C C 1C 1C 1C Kar Alc DNA Cytb CO1 ND1 ITS2 Fig. Date Locality Area P 1 29.07.1999 Kop Geçidi südl. Maden P 1 29.07.1999 Kop Geçidi südl. Maden P 1 29.07.1999 Kop Geçidi südl. Maden 1 29.07.1999 Kop Geçidi südl. Maden 1 29.07.1999 Kop Geçidi südl. Maden 1 29.07.1999 Kop Geçidi südl. Maden 1 1 29.07.1999 Kop Geçidi südl. Maden 1 1 1 29.07.1999 Kop Geçidi südl. Maden 1 29.07.1999 Kop Geçidi südl. Maden 1 29.07.1999 Kop Geçidi südl. Maden 1 29.07.1999 Kop Geçidi südl. Maden 1 29.07.1999 Kop Geçidi südl. Maden P 1 30.07.1999 Gölyurt Geçidi Pazaryolu P 1 30.07.1999 Gölyurt Geçidi Pazaryolu P 1 30.07.1999 Gölyurt Geçidi Pazaryolu 1 30.07.1999 Gölyurt Geçidi Pazaryolu P 1 1 14.08.1999 Pondel Val di Cogne P 1 14.08.1999 Pondel Val di Cogne P 1 14.08.1999 Pondel Val di Cogne P 1 1 1 1 II-35 14.08.1999 Pondel Val di Cogne P 1 14.08.1999 Pondel Val di Cogne P 1 14.08.1999 Pondel Val di Cogne P 1 14.08.1999 Pondel Val di Cogne P 1 14.08.1999 Pondel Val di Cogne P 1 14.08.1999 Pondel Val di Cogne P 1 14.08.1999 Pondel Val di Cogne P 1 14.08.1999 Pondel Val di Cogne P 1 14.08.1999 Pondel Val di Cogne 1 14.08.1999 Pondel Val di Cogne 1 14.08.1999 Pondel Val di Cogne 1 14.08.1999 Pondel Val di Cogne 1 14.08.1999 Pondel Val di Cogne 1 14.08.1999 Pondel Val di Cogne 1 1 1 1 14.08.1999 Pondel Val di Cogne 1 14.08.1999 Pondel Val di Cogne 1 14.08.1999 Pondel Val di Cogne 1 1 1 1 14.08.1999 Pondel Val di Cogne 1 14.08.1999 Pondel Val di Cogne 1 14.08.1999 Pondel Val di Cogne 1 14.08.1999 Pondel Val di Cogne 1 1 1 1 14.08.1999 Pondel Val di Cogne 1 1 1 1 II-36 17.08.1999 Col de Tende Tende 1 17.08.1999 Col de Tende Tende 1 17.08.1999 Col de Tende Tende 1 17.08.1999 Col de Tende Tende 1 17.08.1999 Col de Tende Tende 1 17.08.1999 Col de Tende Tende 1 1 1 30.07.1996 Moscardon Montes Universales 1 1 29.07.1999 M. Perda Liana Lnusei 1 19.05.2000 25 km N Torbat-e-Heydariyeh Kuh-e-Sorkh 1 1 1 II-37 24.05.2000 Voluyeh südöstl. Sari 1 1 02.06.2000 Kotayk, vill. Geghadir 1 1 13.07.2002 25 km W Fulad Mahalleh P 1 1 15.07.2002 Chaman Bid P 1 1 1 16.07.2002 5 km SW Firizi P 1 1 1 II-38 17.07.2002 Kuh-e-Sorkh, Kadkan P 17.07.2002 Kuh-e-Sorkh, Kadkan P 17.07.2002 Kuh-e-Sorkh, Kadkan P 1 1 II-39 17.07.2002 Kuh-e-Sorkh, Kadkan P 1 1 1 21.07.2002 Gardaneh ye Cheri, W Samsami P 21.07.2002 Gardaneh ye Cheri, W Samsami P 1 1 1 II-40 25.07.2002 Dorud, 30 km W Dorud (Paß) E-Seite P 25.07.2002 Dorud, 30 km W Dorud (Paß) E-Seite P 25.07.2002 Dorud, 30 km W Dorud (Paß) E-Seite 1 1 25.07.2002 Dorud, 30 km W Dorud (Paß) E-Seite 1 1 1 II-41 25.07.2002 Dorud, 30 km W Dorud (Paß) E-Seite 1 1 II-42 25.07.2002 Dorud, 30 km W Dorud (Paß) E-Seite P 1 1 1 II-43 27.07.2002 Qamchiyan, 30 km N Chenareh P 27.07.2002 Qamchiyan, 30 km N Chenareh P 27.07.2002 Qamchiyan, 30 km N Chenareh P 27.07.2002 40 km SW Saqqez P 1 1 II-44 27.07.2002 40 km SW Saqqez P 27.07.2002 40 km SW Saqqez P 1 1 1 27.07.2002 40 km SW Saqqez 1 1 1 28.07.2002 20 km E Mahabad 28.07.2002 20 km E Mahabad 1 1 1 29.07.2002 Mahmutabad, W Kaleybar 1 1 1 29.07.2002 Mahmutabad, W Kaleybar P 1 1 1 II-45 31.07.2002 Khalkhal, Gollijeh P 31.07.2002 Khalkhal, Gollijeh P 31.07.2002 Khalkhal, Gollijeh P 1 1 1 31.07.2002 Khalkhal, Gollijeh P 31.07.2002 Khalkhal, Gollijeh P 1 1 31.07.2002 Khalkhal, Gollijeh 1 1 1 31.07.2002 Khalkhal, Gollijeh 1 13.07.1995 Gündüzbey 1 24.07.1995 7-15 km N Saimbeyli 1 1 1 23.07.2002 30 km N Chelgerd (Pass) 1 20.04.1992 Kuschka 1 20.04.1992 Kuschka 1 1 11.07.1994 Aram-Kungei valley, Alytyn Dara river 1 15.07.1994 Chatir Kul, SW Naryn, Ak Bashi range 1 1 22.07.1998 25 km S Song Kul, Molto Tau 1 11.06.1999 Ardakan 1895 1145 624 1877 370 6 312 25 202 Altitude 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 2350 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 900 m 1850 m 1850 m 1850 m 1850 m 1850 m 1850 m 1600 m 1150 m 1700-1800 m 1500-1600 m Province Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Bayburt Erzurum Erzurum Erzurum Erzurum Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Aosta Alpes Maritimes Alpes Maritimes Alpes Maritimes Alpes Maritimes Alpes Maritimes Alpes Maritimes Teruel Sardinia Khorasan Mazandaran 2300 m 1700-2000m 1700-1900m 2100-2500m 2100-2500m 2100-2500m 2100-2500m 2800-3000m 2800-3000m 2100m 2100m 2100m 2100m 2100m 2100m 1800-1900m 1800-1900m 1800-1900m 1800-1900m 1800-1900m 1800-1900m 1800-1900m 1900m 1900m 2200-2400m 2200-2400m 1900m 1900m 1900m 1900m 1900m 1900m 1900m 1000-1100 m 1400-1600 m 3000-3200m 700 m 700 m 3000 m Semnan Khorasan Khorasan Khorasan Khorasan Khorasan Khorasan Bakhtiari Bakhtiari Lorestan Lorestan Lorestan Lorestan Lorestan Lorestan Kordestan Kordestan Kordestan Kordestan Kordestan Kordestan Kordestan Azarbayjan-e-Gharbi Azarbayjan-e-Gharbi Azarbayjan-e-Sharqi Azarbayjan-e-Sharqi Ardabil Ardabil Ardabil Ardabil Ardabil Ardabil Ardabil Malatya Adana Bakhtiari 2000m 95 Explanations: Code = individual code number used for all parts of a specimens (wings, karyological fixations & preparations, DNA-extraction, PCR and sequencing; in most cases made of collector initials, collecting year and a 3-digit number) Wgs = wing vouchers scanned Fix = chromosome fixations available (C) Kar = karyological preparations carried out (P) Alc = alcohol material available ('1'), otherwise dried material DNA = DNA extractions carried out Cytb = Cytochrome b sequenced CO1 = Cytochrome Oxidase I sequence ND1 = NADH dehydrogenase subunit 1 sequenced ITS2 = Internal Transcribed Spacer 2 sequenced Fig. = wings figured on plates Date = collecting date Locality =collecting locality (incl. altitude, Province, country - 164 - West Transalai Tianshan Inner Tianshan Yazd Country Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Turkey Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy Italy France France France France France France Spain Italy Iran Iran Armenia Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Iran Turkey Turkey Iran Turkmenistan Turkmenistan Kirgizia Kirgizia Kirgizia Iran Appendix 3: Sequence data AD98001 AD98009 AD98012 AD98018 AD98024 AD98029 AD98036 DS00001 DS00005 DS01001 JC00029 JC00039 JC00040 JC00042 JC00043 JC00045 JC00046 JC00051 JC00055 JC00057 JC00061 JC00062 JC00063 JC01014 JC02001 JM00001 MW00001 MW00015 MW00017 MW00020 MW00024 MW00032 MW00044 MW00051 MW00056 MW00058 MW00059 MW00060 MW00064 MW00072 MW00076 MW00087 MW00101 MW00102 MW00110 MW00116 MW00119 MW00125 MW00127 MW00129 MW00140 MW00151 MW00157 MW00176 MW00177 MW00178 MW00179 MW00183 MW00185 MW00186 MW00189 MW00226 MW00229 MW00231 MW00232 MW00234 MW00259 MW00262 ....|....| 5 TAGCGAAAAT NNNNNNNNNN TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT TCGCGAAAAT TCGCGAAAAT NNNNNNNNNN NNNNNNNNNN TCGCGAAAAT TAGCGAAAAT CAGCGAAAAT TCGCGAAAAT TAGCGAAAAT TCGCGAAAaT TCGCGAAAAT NNNNNAAAAT TAGCGAAAAT NNNNNNNNAT TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT NNNNNNNNNN TNGCGAAAAT NNNNNNNNNN TAGCGAAAAT TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT NNNNNNNNNN NNNNNNNNNN TAACGAAAAT TCGCGAAAAT TCGCGAAAAT NNNNNNNNNN TGGCGAAAAT NNNNNNNNNN TCGCGAAAAT TCGCGAAAAT NNNNNNNNNN TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT TCGCGAAAAT NNNNNNNNNN TAGCGAAAAT TAGGGAAAAT TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TCGNGAAAAN TCGCGAAAAT NNNNNNNNNN TAGCGAAAAT NNNNNNNNNN NGGCGAAAAT TAGCGAAAAT NNNNNNNNNN TCGCGAAAAT NNNNNNNNNN ....|....| 15 GACTTTTTTC NNNNNNNNNC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN NNNNNNNNTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTATTC NNNNNNNNNN GACTTTTTTC GACTTTTTTC GAaTTTATTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN NNNNNNNNNN GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC NNNNNNNNNN GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC NNNTTTTTTC ....|....| 25 TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT NNNNNNNNAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT CACAAATCAT CACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT NACAAATCAT NACAAATCAT NNCAAATCAT TACAAATCAT NNNAAATCAt TACAAATCAT CACAAATCAT AACAAATCAT TACAAATCAT tACAAATCAT TACAAATCAT AACAAATCAT TACAAATCAT NNNNAATCAT NNNNNNNNNN TACAAATCAT TACAAATCAT TACAAATCAT NNNNAATCAT CACAAATCAT CACAAATCAT TACAAATCAT TACAAATCAT NNNNAATCAT TACAAATCAT AACAAATCAT TACAAATCAT TACAAATCAT TNNNAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAACCAT CNCAAATCAT CACAAATCAT CACAAATCAT NNCAAATCAT NACAAATCaT NNNNAATCAT TACAAATCAT TACAAATCAT NNNNNNNCAT TACAAATCAT TACAAATCAT Cytochrome Oxidase I (COI) ....|....| 35 AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATCG AAAGATATCG AAAGATATCG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG ....|....| 45 GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GGACATTGTA GAACGTTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATtATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACTTTATA GAACATTATA GAACATTATA GAACATTATA GAACTTTATA GAACACTATA GNACACTATA GAACACTATA GAACACTATA GGACATTATA GAACATTATA GGACATTATA GAACATTATA GAACATTATA GGACATTATA GGACATTATA GAACACTATA GAACATTATA GAACTTTATA GAACATTATA GAACACTATA GaaCATTATA GGACATTGTA GAACATTATA GAACACTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACACTATA GAACATTATA GGACATTATA GGACATTATA ....|....| 55 TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATCTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTCATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT TTTCATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATCTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT CTTTATTTTT ....|....| 65 GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGGATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGGG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGTATTTGAG GGTATTTGGG GGAGTTTGAG GGTATTTGGG GGAGTTTGAG GGAATTTGGG GGAATTTGAT GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAATTtGAG GGTATTTGGG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAGTTTGAG GGAGTTTGAG GGAGTTTGAG GGAGTTTGAG GGAGTTTGAG GGTATTTGGG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG GGAGTTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGGATTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGGG GGAGTTTGAG GGGGTTTGAG GGGGTTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG ....|....| 75 CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAAT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CGGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATGGT CAGGAATAGT CAGGAATGGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGGATAGT CAGGAATAGT cTGGAATAGT CAGGAATGGT CCGGAATAGT CAGGGATAGT CTGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CGGGAATAGT CTGGAATAAT CAGGAATAGT CAGGAATAGT CAGGAATAGt CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATGGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT ....|....| 85 AGGaACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC GGGAACATCC GGGAACATCC GGGAACATCC GGGAACATCT AGGAACATCC AGGAACATCA AGGGACATCT AGGAACATCT AGGAACATCA AGGaACATCT AGGAACATCT AGGAACATCT AGGAACATCA AGGAACATCT AGGAACATCT AGGAACATCA AGGAACATCT AGGAACATCA AGGAACATCT AGGAACTTCA AGGAACATCC AGGAACATCT GGGAACATCT TGGAACCTCT AGGAACATCT AGGAACATCT AGGAACATCT TGGAACTTCT AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC GGGAACATCC GGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCT TGGAACTTCC AGGAACATCC AGGAACATCC AGGAACATCA GGGAACATCC GGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCA AGGAACATCA AGGAACATCA GGGAACATCT GGGAACATCT GGGAACATCT AGGAACATCT AGGAACATCC GGGAACATCT GGGAACATCT AGGAACATCC AGGAACATCC GGGAACATCC GGGAACATCC 165 ....|....| 95 CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAaTTT CTAAGAATTT TTAAGAATTT CTAAGAATTT TTAAGAATTC TTAAGAATTT CTAAGAATTT TTAAGAATTC TTAAGAGTTT TTAAGAGTTT TTAAGAGTTT TTAAGAATTC TTAAGAATTT CTAAGAATTT TTAAGAATTC CTAAGAATTT TTAAGAATTC TTAAGAGTTT TTAAGAATAA CTAAGAaTTT CTAAGAATTT TTAAGAGTTT TTAAGaATTT CTAAGAATTT TTAAGAATTT CTAAGAATTT TTAAGAATTC CTAAGAATTT NTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATCT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATtT CTAAGAATTT CTTAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAGTTT TTAAGAGTTT TTAAGAGTtT TTAAGAGTTT TTAAGAATTC CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT ....|....| 105 TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATCCGTAT TAATTCGTAT TAATCCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TTATTCGAAC TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTCT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTCT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TGATTCGTAT TGATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGAAT TAATTCGCCT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATCCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT ....|....| 115 AGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA AGAATTGAGA AGAATTAAGA AGAACTGAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA AGAATTGAGA AGAATTAAGA GGAATTAAGA AGAATTGAGA GGAATTGAGA GGAATTGAGA GGAATTGAGA AGAATTGAGA GGAATTAAGA GGAATTAAGA AGAATTGAGA GGAATTAAGA AGAATTGAGA AGAATTGAGA TGAATTAGGT GGAATTAAGA AGAATTGAGA GGAATTGAGA TGAATtAGGT GGAATTAAGA AGAACTAAGA AGAATTGAGA TGAATTAGGA AGAATTGAGA AGAATTGAGA AGAATTGAGA AGAATTGAGA AGAATTAAGA GGAATTAAGA AGAATTAAGA GGAATTAAGA GGAATTAAGA AGAATTGAGA AGAATTAAGA AGAATTGAGA AGAATTAGGA TGAATTAGGT AGAATTAAGA AGAATTGAGA AGAATTAAGA AGAACTGAGA AGAATTAAGA AGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA GGAATTGAGA GGAATTGAGA GGAATTGAGA GGAATTGAGA AGAATTGAGA GGAATTAAGA GGAATTAAGA AGAATTGAGA AGAATTAAGA AGAATTGAGA AGAATTGAGA ....|....| 125 ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCCGGAT ACTCCCGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCAGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGAAT ACTCCTGGAT ACTCCTGGAT ACTCCAGGAT ACTCCAGGAT ACTCCAGGAT ACTCCTGGAT ATTCCGGGAT ATTCCAGGAT ACCCCTGGAT ATTCCAGGAT ACACCTGGAT ACCCCAGGAT AATCCAGGTT ACTCCNGGAT ACTCCTGGAT ACTCCAGGAT AcCCCAGGAT ATTCCAGGAT ATTCCTAGAT ACTCCTGGAT ACTCCAGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCCGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTTCAGGGT ACTCCTGGAT ACTCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACCCCTGGAT ACCCCTGGAT ACCCCTGGAT ACTCCAGGAT ACTCCAGGAT ACTCCAGGAT ACTCCAGGAT ACTCCGGGAT ACTCCCGGAT ACTCCCGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ....|....| 135 CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CcTTAATTGG CCTTAATTGG CCTTAATTGG CTTTGATTGG CTTTGATTGG CTTTGATTGG CcTTAATTGG CTTTAATTGG CTTTAATTGG CATTAATTGG CTTTAATTGG CATTAATTGG CTTTGATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CTTTGATTGG CTTTAATtGG CTTTAATTGG CTTTAATTGG CCTTAATTGG CTTTAATTAG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CTTTAATtGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTGATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTGATTGG CTTTGATTGG CTTTGATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CTTTAATTGG CTTTAATTGG ....|....| 145 AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGACGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA TGATGATCaA AGATGATCAA TGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGAtGATCaA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGACGATCAA GAATGATCAA GAATGATCAA AGATGATCAA AGATGATCAA TGATGATCAA TGATGATCAA ....|....| 155 ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTACAAtA ATTTACAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATCTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ....|....| 165 CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTATTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATTGTCAC CCATCGTTAC CCATCGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATCGTTAC CTATTGTTAC CTATCGTTAC CCATCGTTAC CTATTGTAAC CTATTGTTAC CTATTGTTAC CCATCGTTAC CTATTGtAAC CTATTGTTAC CTATTGTAAC CTATTGTTAC CTATTGTAAC CTATTGTCAC CTATTGTCAC CTATTGTCAC CTATTGTCAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTGTTAC CTATCGTAAC CTATTGTTAC CTATTGTCAC CTATTGTTAC CTATTATTAC CTATTGTTAC CTATTGTCAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATCGTTAC CCATCGTTAC CCATCGTTAC CCATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTGTTAC CTATTGTTAC CTATTGTTAC ....|....| 175 AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCACATGCT AGCCCATGCG AGCTCATGCA AGCTCATGCA TGCTCATGCT AGCTCATGCA AGCTCACGCA AGCTCATGCA TGCTCATGCT AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA TGCTCATGCT AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA Appendix 3: Sequence data MW00267 MW00269 MW00290 MW00302 MW00316 MW00326 MW00327 MW00328 MW00330 MW00335 MW00347 MW00348 MW00358 MW00393 MW00409 MW00412 MW00424 MW00444 MW00452 MW00469 MW00476 MW00497 MW00498 MW00504 MW00517 MW00525 MW00530 MW00539 MW00547 MW01001 MW01009 MW01011 MW01014 MW01018 MW01019 MW01022 MW01023 MW01025 MW01027 MW01034 MW01039 MW01048 MW01053 MW01059 MW01061 MW01072 MW01078 MW01083 MW01092 MW01103 MW01105 MW01107 MW01114 MW01116 MW01120 MW02001 MW02006 MW02007 MW02008 MW02009 MW02021 MW02024 MW02025 MW02028 MW02031 MW02033 MW02034 MW98002 ....|....| 5 TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT TTGCGAAAAT NNNNNNNNNN TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TCGCGAAAAT NNNNNNNNNN NNNNNNNNNT TCGCGAAAAT NNNNNNNNNN TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT NNNNNNNNNN TCGCGAAAAT NNNNNNNNNN TCGCGAAAAT NNNNNNNNNN TAGCGAAAAT TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT TCGCGAAAAT TCGCGAAAAT NNNNNNNNNT TCGCGAAAAT TCGCGAAAAT TTGCGAAAAT NNNNNNNNNN TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT AAGCGAAAAT NNNNNNNNNT TCGCGAAAAT NNNNNNNNNN TCGCGAAAAT TTGGGAAAAN NNNNNNNNNN TCGCGAAAAT NNNNNNNNNN TTGCGAAAAT NNGCGAAAAT TTGCGAAAAT NNNCGAAAAT NNNNNNNNAT ATGCGAAAAT TTGCGAAAAT TAGCGAAAAT TCGCGAAAAT TAGCGAAAAT NAGCGAAAAT TAGCGAAAAT TTGCGAAAAT NNNNNNNNNT NNNNNNNAAT NNNNNNNNNN TAGCGAAAAT NNNNNNNNNN TAGCGAAAAT TAGCGAAAAT TAGCGAAAAT ....|....| 15 GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNTC GATTATATTC GACTTTTTTC GACTTTTTNN GACTTTTTTC NNNNNNTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC NNNNTTTTTC GACTTTTTTC NNNNNNNNNC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GANTNNNTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC GACTTTTTTT GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNCTTTTTTC GACTTTTTTC AACTTTTTTN NNNNNNNNNN GACTTTTTTC NNNNNNNNTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTATTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GATTTTTTTC GAATTTATTC GACTTTTTTC GACTTTTTTN GATTATTTTC GACTTTATTC NACTTTTTTC GACTTTTTTC NACTTTTTTC GACTTTTTTC GACTTTTTTC GAnTTTTTTC ....|....| 25 TACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT TACAAATCAT AACAAATCAT TACAAATCAT NNNNAATCAT TACAAACCAT TACAAATCAT TACAAATCAT TACAAATCAT NNNNAATCAT TACAAATCAT NNNNAATCAT TACAAATCAT NNNNNNNNAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT TACAAATCAT tACAAATCAT CACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT TACAAATCAT NNNAAATCAT AACAAATCAT CACAAATCAT TACAAATCAT TACAAACCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TAAAAATCAT TACAAATCAT CACAAATCAT TACAAATCAT CaCAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT AACAAATCAT TACAAATCAT TACAAATCAT AACAAATCAT AACAAATCAT NACAAATCAT TACAAATCAT TACAAATCAT AACAAATCAT AACAAATCAT AACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT Cytochrome Oxidase I (COI) ....|....| 35 AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGACATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATaTTG AAAGATATTG ACAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAaGATATTG AAAGATATTG AAAGATATTG AAAGATATTG ....|....| 45 GGACATTATA GAACATTATA GGACATTATA GAACATTATA GAACACTATA GAACATTATA GGACATTATA GAACATTATA GAACATTATA GGACATTATA GAACATTATA GGACATTATA GAACATTATA GGACATTATA GNACATTATA GAACATTATA GAACATTATA GGACATTATA GGACATTAtA GAACATTATA GGACATTATA GAACTTTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GGACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACTTTATA GAACATTATA GAACTTTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACNTTATN GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACTTTATA GAACATTATA GAACTTTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAaCTTTATA GAACATTATA GAACATTATA GAACATTATA ....|....| 55 TTTTATTTTT TTTTGTTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTGTTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTGTTTTT TTTTATTTTT TTTTATTTTt CTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTCATTTTT TTTTATTTTT TTTTATTTTT TTTCATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTCATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATCTTT TTTTATTTTC TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTAtTTTT TTTTATTTTT TTTTATTTTT TTTCATTTTT TTTTATTTTT TTTTATTTTT ....|....| 65 GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGTATTTGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGTATTTGAG GGAGTTTGAG GGAGTTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG NNNNTTTGAG GGTATTTGGG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGGG GGGATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAA GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGtATTTGAG GGAATTTGAG GAAATTTGAG GGTATTTGAG GGAATTTGGG GGAATTTGAG GGAATTTGAG GGGATTTGAG GGGATTTGAG GGAATTTGGG GGAATTTGAG GGAATTTGAT GGGATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGGATTTGAG GGAATTTGAG GGAATTTGAG GGAaTTTGAT GGAATTTGGG GGAATTTGAG GGAATTTGAG GGTATTTGAG GGAATTTGAG GGAATTTGAG ....|....| 75 CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGGATAGT CAGGAATAGT CAGGAATAGT CGGGAATAGT CGGGAATAGT CAGGAATAGT CGGGAATAGT CAGGGATAGT CGGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CGGGAATAGT CAGGAATAGT CAGGAATATT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CGGGAATAGT CGGGAATAGT CAGGAATAGT CGGGAATAGT CAGGAATAGT CAGGAATATT GAGGAATATT CGGGAATAGT CAGGAATACT CGGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CNGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CGGGAATAGT CAGGAATATT CAGGAATAGT CAGGGATAGT CTGGAATAGT CAGGTATATT CAGGAATAGT CAGGAATATT CAGGAATAGT CAGGAATATT CAGGAATATT caGGAATATT CAGGAATAGT CAGGAATAGT CAGGAATATT ....|....| 85 AGGAACATCC AGGAACATCC GGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCA AGGAACATCC AGGAACATCC AGGAACATCA GGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC GGGAACATCC AGGAACATCA AGGAACATCC AGGAACATCC GGGAACAtCC AGGAACATCC AGGAACATCC AGGAACATCT AGGAACATCC AGGAACATCC AGGAACATCT GGGAACATCT GGGAACATCA AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCT AGGAACATCC AGGAACATCC GGGAACATCA AGGAACTTCA AGGAACATCA AGGAACATCT AGGAACATCC AGGAACATCC AGGaACATCT AGGAACATCT AGGAACATCC AGGAACAtCC AGGAACATCT AGGAACATCC AGGAACATCC AGGAACATCC GGGAACATCC AGGAACATCT GGGAACATCT AGGAACTTCA GGGAACATCC AGGAACATCT AGGAACATCC GGGAACATCC AGGAACCTCT AGGAACATCA AGGAACCTCC AGGAACATCA AGGAACTTCA AGGAACATCT GGGAACATCA AGGAaCATCT AGGAACATCT AGGAACATCT AGGAACATCT 166 ....|....| 95 CTAAGAATTT CTAAGAATTT TTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATCT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT CTAAGAATCT TTAAGAATTC CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT TTAAGAATCT TTAAGAATTC CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT CTAAGAATTT TTAAGAGTTT CTAAGAATTT TTAAGAATTT TTAAGAATTT TTAAGAATTT TTAAGAATTC TTAAGAATTC CTAAGAATTT CTAAGAATTT TTAAGAaTTT CTAAGAATTT CTAAGAATTT TTAAGAATTT TTAAGAGTTT CTAAGAGTTT TTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAGTTT CTAAGAATTT TTAAGAATAA CTAAGAATTT TTAAGAATCT TTAAGAATTT TTAAGAATTC TTAAGAATTT TTAAGAATCT TTAAGAATTT TTAAGAATTT TTAAGAATAA TTAAGAATTT TTAAGAATTT TTAAGaATTT TTAAGAATTT TTAAGAATCT TTAAGAATTT ....|....| 105 TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGAAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGAAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGAAT TAATTCGAAT TAATTCGTAT TAATTCGAAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TTATTCGAAC TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTCT TAATTCGTAT TAATTCGTCT TAATTCGAAT TTATTCGAAC TAATTCGTAT TAATTCGAAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGAAT ....|....| 115 AGAATTAAGA AGAATTGAGA AGAATTAAGA AGAATTGAGA AGAATTGAGA AGAATTAAGA GGAATTGAGA AGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA AGAACTGAGA AGAATTGAGA GGAATTGAGA AGAATTAAGA AGAATTGAGA AGAATTGAGA AGAATTAAGA AGAGCTGAGA AGAATTAAGA AGAATTAAGA AGAATTAGGA GGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA AGAATTGAGA AGAATTAAGA GGAATTAAGA AGAATTAAGA AGAATTAAGA GGAATTGAGA AGAATTAAGA AGAATTGAGA AGAGTTAGGA AGAATTAGGA AGAATTGAGA AGAATTAGGA AGAATTAAGA GGAATTAAGA GGAATTAAGA GGAATTAAGA AGAATTAAGA AGAATTAAGA GGAATTGAGA GGAATTAAGA AGAATTGAGA AGAATTAAGA AGAATTAAGA GGAATTGAGA GGAATTAAGA TGAATTAGGT AGAATTAAGA AGAATTAGGA AGAATTGAGA AGAATtAAGA TGAATTAGGT AGAGTTAGGA TGAATTAGGT AGAATTAGGT TGAATTAGGT AGAATTAGGA AGAGTTAGGA AGAATTAGGA GGAATTAAGA AGAATTAAGA AGAATTAGGA ....|....| 125 ACTCCTGGAT ATTCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCTGGAT ACCCCAGGAT ACCCCTGGAT ATTCCTGGAT ACCCCTGGAT ACTCCTGGGT ACTCCTGGAT ACTCCGGGAT ACTCCTGGAT ACCCCTGGAT ACTCCTGGGT ACCCCTGGAT ATTCCTGGAT ACTCCTGGAT ACTCCGGGAT ACCCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCCGGAT ACTCCTGGAT ACACCTGGAT ACTCCTGGAT ACTCCTGGAT ATTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGAAT ACCCCTGGAT ACTCCGGGAT ACCCCTGGAT ACTCCTGAAT ACTCCAGGAT ACACCAGGAT ACCCCTGGAT ACACCAGGAT ACCCCTGGAT ACTCCTGGAT ATTCCAGGAT ACTCCTGGAT ACCCNTGGAT ACTCCTGGAT ACTCCGGGAT ACTCCTGGAT ACTCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCAGGAT ACTCCTGGAT AATCCAGGTT ACCCCTGGAT AATCCCGGAT ATTCCTGGAT ACTCCTGGAT ACCCCAGGAT ACACCGGGAT ACTCCAGGAT ACTCCTGGAT AATCCAGGTT ACACCCGGAT ACTCCAGGAT ACACCTGGAT ATTCCAGGAT ACTCCTGGAT ACCCCAGGAT ....|....| 135 CCTTAATTGG CCTTAATTGG TCTTAATTGG CCTTAATTGG CCTTAATTGG CATTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATCGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATCGG CATTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CTTTAATTGG CTTTAATTGG CTTTAATTGG CTTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CTTTGATTGG CTTTGATTGG CCTTAATTGG CTTTAATTGG CTTTTATTGG CATTGATTGG CTTTAATTGG CTTTGATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CTTTGATTGG CTTTAATTGG CTTTGATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CTTTGATTGG CTTTAATTGG CCTTAATTGG CTTTAATTGG CTTTGATTGG CTTTAATTGG TCTTAATTGG CTTTAATCGG CTTTAATTGG CATTAATTGG CTTTAATTGG CATTAATTGG CTTTAATTGG CATTAATTGG CTTTAATTGG CATTAATTGG CTTTAATTGG CTTTAATTGG CATTAATTGG ....|....| 145 AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA TGATGACCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA TAATGATCAA AAATGATCAA AGATGATCAA TGATGATCAA TGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA GGATGATCAA AGATGATCAA TGATGATCAA TGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA GGATGATCAA AGACGATCAA AGATGATCAA AGATGATCaA TGATGATCAA AGATGATCAA TGATGATCAA TGATGATCAA AGATGATCAA AGATGATCAA TGATGATCAA AGATGATCaA AGATGATCAA TGATGATCAA AGATGATCAA ....|....| 155 ATTTATAACA ATTTATAATA ATTTACAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAACA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTACAATA ATTTATAATA ATTTATAATA ATCTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ....|....| 165 CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTGTCAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTATTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATCGTTAC CTATTGTTAC CTATTGTTAC CTATTATTAC CTATTGTTAC CTATTGTTAC CAATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATTGTAAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATCGTTAC CTATTGTTAC CTATTGTCAC CTATTGTAAC CAATCGTCAC CTATCGTTAC CCATCGTAAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATCGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATCGTTAC CTATTGTTAC CTATTGTAAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTtAC CTATTGTAAC CTATTGTCAC CTATTGTAAC CTATTGTAAC CTATTGTAAC CTATTGTAAC CTATTGTAAC CTATTGTAAC CTATTGTTAC CCATTGTAAC CTATTGTTAC ....|....| 175 AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCT AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCG AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCT AGCTCATGCT AGCTCATGCA AGCTCATGCT AGCTCATGCA AGCCCATGCG AGCTCATGCA AGCCCATGCG AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCG AGCTCATGCA AGCTCATGCA AGCTCACGCA AGCTCATGCA AGCCCATGCG AGCACATGCT AGCTCATGCA AGCTCATGCT AGCTCATGCA AGCTCATGCA TGCCCATGCT AGCTCATGCT TGCACATGCC AGCTCATGCT AGCACATGCT AGCTCATGCT AGCTCATGCT AGCTCATGCT AGCTCATGCA AGCTCATGCT AGCTCATGCT Appendix 3: Sequence data MW98008 MW98009 MW98020 MW98029 MW98049 MW98079 MW98084 MW98089 MW98094 MW98097 MW98103 MW98106 MW98128 MW98129 MW98133 MW98136 MW98138 MW98139 MW98154 MW98162 MW98170 MW98172 MW98180 MW98183 MW98185 MW98189 MW98203 MW98205 MW98212 MW98220 MW98228 MW98230 MW98235 MW98240 MW98261 MW98264 MW98265 MW98270 MW98274 MW98278 MW98284 MW98285 MW98294 MW98313 MW98315 MW99001 MW99006 MW99009 MW99013 MW99014 MW99018 MW99028 MW99038 MW99042 MW99045 MW99047 MW99053 MW99057 MW99058 MW99059 MW99060 MW99061 MW99068 MW99080 MW99084 MW99094 MW99095 MW99097 ....|....| 5 TTGCGAAAAT TAGCGAAAAT TCGCGAAAAT TTGCGAAAAT TCGCGAAAAT TCTCGAAAAT TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TAGGGAAAAT TCGCGAAAAT NNNNNNNNNN TAGCGAAAAT TTGCGAAAAT TAGCGAAAAT TCGCGAAAaT TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT NNNNNNNNNN TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TCGCGAAAAT TAGCGAAAAT TTGCGAAAAT TAGCGAAAAT TCGCGAAAAT CAGCGAAAAT NNNNNNNNNN TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT TTGCGAAAAT TCGCGAAAAT TAGCGAAAAT NNNNNNNNNN TAGCGAAAAT TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT NNNNNNNNNN NNNNNAAAAT TTGCGAAAAT NNNNNAAAAT TCGCGAAAAT TAGCGAAAAT TCGCGAAAAT TAGCGAAAAT NNNNNNNNNN NNNNNAAAAT NNNNNNNNNN TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT TCGCGAAAAT NNNNNNNNNN TAGCGAAAAT ....|....| 15 GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GATTTTTTTC GATTTTTTTC GACTTTTTTC NNNNNNNNNN GATTTTTTTC GACTTTTTTN GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNCTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GATTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNCTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC ....|....| 25 NACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT TACAAATCAT TACaAATCAT CACAAATCAT TACAAATCAT AACAAATCAT TACAAATCAT TACAaATCAT NACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TNCAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCaT TACAAATCAT TACAAATCAT TACAAATCAT NNNNNNNNNN TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT AACAAATCAT CACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT NNNAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT cACAAATCAT AACAAATCAT NNNNNNNNNN CACAAATCAT TACAAATCAT AACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAACCAT TACAAATCAT NNCAAATCAT CACAAATCAT AACAAATCAT AACAAATCAT TACAAACCAT NACAAATCAT TACAAATCAT Cytochrome Oxidase I (COI) ....|....| 35 AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG NNNNNNNNNN AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGACATTG AAAGATATCG AAAGATATCG AAAGACATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAAATATTG NNNNNNNNNN AAAGACATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG ....|....| 45 GAACATTATA GAACATTATA GAACATTATA GGACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACTTTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACaTTATA GAACATTATA GaACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GaACATTATA GAACATTATA GAACATTATA GAACATTATA NNNNNNNNNN GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAAcaTTATA GAACATTATA GAANTTTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAaCATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAAGTTTATA GAACATTATA GAACATTATA NNNNNNNNNN GAACATTATA GAAcaTTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACTTTATA GAACTTTATA GaACATTATA GAACATTATA GAACATTATa ....|....| 55 TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATCTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATCTTT TTTTATTTTT TTTTANTTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT NNNNNNNNNN TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTCATTTTT TTTTATTTTT TTTTATCTTT TTTTAATTTT TTTTATTTTT CTTTATTTTT NNNNNNNNNN TTTTATTTTT TTTTAATTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTAATTTT TTTTATTTTT TTTTATTTTT TTTCATTTTT ....|....| 65 GGAATTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGGG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAGTTNGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGGATTTGAG GGNNTTTGAG NNNNNNNNNN GGAGTTTGAG GGAATTTGAG GGAGTTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGTNTTTGAG GGAGTTTGAG GGGNTTTGAG GGAATTTGAG GGTATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG GGTATTTGAG GGAATTTGAG GGAATTTGAG GGNNTTTGAG GGAATTTGAG GGAATTTGAG NNNNNNNNNN GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGANTTTGAG GGAATTTGAG GGAATttGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG NNNNTTTGAG GGNATTTGAG GGAATTTGAG GGTATTTGAG ....|....| 75 CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAAtAGt CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CTGGAATAAT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGG CAGGAATAAT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT NNNNNNNNNN CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CGGGAATAGT CTGGAATAAT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATATT CAGGAATAGT CAGGAATAGT CGGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT NNNNNNNNNN CAGGAATAGT CAGGAATAGT CAGGAATATT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATGGT CAGGAATATT CAGGTATATT CAGGAATAGT CAGGAATAGT CAGGAATAGT ....|....| 85 AGGAACATCC AGGAACATCC GGGAACATCT AGGAACATCC GGGAACATCC GGGAACATCC AGGAACATCT GGGAACATCC TGGAACTTCT GGGAACATCC AGGAACATCC GGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCA AGGAACATCC GGGAACATCC AGGAACATCC GGGAACATCC AGGAACATCC AGGAACATCC GGGAACATCT GGGAACATCC GGGAACATCC NNNNNNNNNN GGGAACATCC AGGAACATCC AGGAACATCC GGGAACATCT AGGAACATCT AGGAACATCC TGGAACTTCT AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCA GGGAACATCA AGGAACATCA AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC GGGAACATCT AGGAACATCT AGGAACATCC GGGAACATCC AGGAACATCT AGGAACATCC AGGAACATCT NNNNNNNNNN AGGAACATCC AGGAACATCC AGGAATATCT AGGAACATCT AGGAACATCC AGGAACATCC GGGAACATCC AGGAACATCA AGGAACATCC GGGAACATCC AGGAACATCT AGGAACATCT AGGAACATCA AGGAACATCA AGGAACATCC AGGAACATCT 167 ....|....| 95 TTAAGAATTT TTAAGAATTT CTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAGTTT CTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT TTAAGAATTT TTAAGAATTC CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGaATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT NNNNNNNNNN CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATCT TTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT TTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT TTAAGAATTT TTAAGAATTT NNNNNNNNNN CTAAGAATTT TTAAGAATTT TTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAGTTT TTAAGAATTT TTAAGAATCT CTAAGAaTTT CTAAGAATTT TTAAGAATTT ....|....| 105 TAATTCGTAT TAATTCGTAT TAATTCGTAT TGATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTCT TAATTCGTAT TAATCCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT NAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTCT TAATTCGATT TAATTCGTAT TAATTCGTAT TAATTCGATT TAATTCGAAT TAATTCGTAT TGATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGAAT TAATTCGTAT TAATTCGTAT NNNNNCGTAT TAATTCGATT TAATTCGTAT TAATTCGAAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT ....|....| 115 AGAATTGAGA AGAATTGAGA GGAATTAAGA GGAATTAAGA AGAATTAAGA GGAATTAAGA AGAATTGAGA GGAATTAAGA TGAATTAGGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA AGAATTAAGA AGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA GGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA GGAATTAAGA AGAATTAAGA AGAATTAAGA TGAATTAGGT AGAGCTAAGG AGAATTGAGA AGAATTGAGA AGAGCTAAGA AGAGTTAGGA AGAATTGAGA GGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA GGAATTAAGA GGAATTAAGA AGAATTAAGA AGAATTAAGA GgAATTAAGA AGAATTAAGA GGAATTAAGA GGAATTAAGA AGAGCTAAGG AGAATTAAGA AGAATTAAGA AGAGTTAAGA AGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA AGAATTAAGA GGAATTGAGA AGAATTAGGA AGAGTTAGGA AGAATTAAGA AGAATTGAGA GGAATTAAGA ....|....| 125 ACTCCTGGAT ACTCCTGGAT ACCCCCGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCAGGAT ACTCCTGGAT ACACCAGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGAAT ACCCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT aCTCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCCGGAT ACTCCTGGAT ATCCCTGGAT ACTCCAGGAT ACCCCAGGAT ACTCCTGGAT ACTCCTGGAT ACCCCAGGAT ACTCCAGGAT ACTCCAGGGT ACTCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCCGGAT ATTCCAAGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACCCCTAAAT ACCCCTGGAT ATtCCAGGAT ACCCCAGGAT ACCCCTGGAT ACTCCAGGAT GTTCCTGGAT ACTCCCGGAT ACTCCTGGAT ACTCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCAGGaT ACACCTGGAT ACACCAGGAT ACCCCTGGAT ACTCCCGGAT ATTCCAGGAT ....|....| 135 CCTTAATTGG CCTTAATTGG CCTTAATTGG TCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTGATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CATTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATCGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CTTTAATTGG CTTTAATTGG CTTTAATTGG CATTAATTGG CTTTAATTGG CCTTAATTGG CATTAATTGG CTTTAATTGG CTTTAATTGG TCTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CTTTAATCGG CTTTaATTGG CATTAATTGG CTTTAATTGG ATTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CTTTAATTGG CATTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG ....|....| 145 AGATGATCAA AGATGATCAA GAATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA GAATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA GAATGATCAA tGATGATCAA AGATGATCAA AGATGATCAA TGACGACCAA AGATGATCAA AGATGATCAA TGATGACCAA TGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA GAATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGANGATCAA AGATGATCAA AGATGATCAA AGATGaTCAA TGATGACCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA ....|....| 155 ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATCTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTACAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACT ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATaATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ....|....| 165 CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTGTTAC CCATCGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTGTCAC CTATTGTTAC CTATCGTtAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATCGTTAC CTATTGTTAC CTATTGTAAC CTATTGTCAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTGTAAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTGTTAC CTATTGTCAC CCATTGTTAC CTATTGTTAC CTATTGTCAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATTGTTAC CTATTGTAAC CTATTGTCAC CTATTGTtAC CTATTGTTAC CTATTGTTAC ....|....| 175 AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA TGCTCATGCT AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA TGCTCATGCT AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCT AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCT AGCTCATGCA AGCTCACGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCT AGCtCATGCT AGCCCATGCA AGCTCACGCA AGCTCATGCA Appendix 3: Sequence data MW99102 MW99104 MW99105 MW99124 MW99128 MW99134 MW99135 MW99140 MW99141 MW99158 MW99163 MW99164 MW99165 MW99170 MW99174 MW99187 MW99196 MW99203 MW99221 MW99226 MW99240 MW99247 MW99263 MW99264 MW99274 MW99276 MW99286 MW99289 MW99292 MW99301 MW99303 MW99309 MW99314 MW99319 MW99320 MW99341 MW99344 MW99353 MW99357 MW99372 MW99374 MW99376 MW99380 MW99381 MW99382 MW99393 MW99398 MW99406 MW99407 MW99408 MW99413 MW99422 MW99425 MW99430 MW99435 MW99448 MW99449 MW99465 MW99469 MW99471 MW99473 MW99476 MW99479 MW99494 MW99501 MW99508 MW99514 MW99515 ....|....| 5 TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TTGCGAAAAT TAGCGAAAGT NNNNNNNNNN NNNNNAAAAT TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT TCGCGaAAAT NNNNNNNNNN TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT TCGCGAAAAT TAGCGAAAAT TCGCGAAAAT TCGCGAAAAT NNNNGAAAAT TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT NNNNGAAAAT TAGCGAAAAT TAGCGAAAAT NNNNNNNNNT NNNNNAAAAT TCGCGAAAAT TCGCGAAAAT NNNNNNNNNN TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TAGCGAAAAT TCGCGAAAAT TCGCGAAAAT TCGCGAAAAT NNNNNNNNNN TAGCGAAAAT TAGCGAAAAT TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT NNNNNNNNNN TAGCGAAAAT TAGCGAAAAT CGGCGAAAAT TCGCGAAAAT TCGCGAAAAT NNNNNNNNNN TAGCGAAAAT TCGCGAAAAT TAGCGAAAAT TCGCGAAAAT TAGCGAAAAT TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TTGCGAAAAT TAGCGAAAAT ....|....| 15 GACTTTTTTC GACTTTTTTC GACTTTTTTC AAATTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTtN GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNC GACTTTTTTC GACTTTTTTC TACTTTTTTN GACTTTTTTC GACTTTTTTC NNNNNNNNTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTA ....|....| 25 TACAAATCAT CACAAATCAT CACAAATCAT NNNNAATCAT CACAAATCAT TACAAATCAT TACAAATAAT TACAAATCAT CACAAATCAT TACAAATCAT CACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT TACAAATCAT NNcAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT NACaaATCAT TACAAATCAT TACAAATCAT TACAAAtCAT TACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACTAATCAT CACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT CACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT NACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAaATCAT TACAAATCAT TACAAACCAT TACAAACCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT AACAAATCAT Cytochrome Oxidase I (COI) ....|....| 35 AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATNTNG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATCG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG ....|....| 45 GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACANTATA GAACATTATA GAACATTATA GAACTTTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GGACATTATA GGACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GaACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACTTTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GGACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACTTTATA ....|....| 55 TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATCTTT TTTTATTTTT TTTTATTTTT TTTTATCTTT TTTtaTTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATNTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTt TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATCTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT ....|....| 65 GGAGTTTGGG GGANTTTGGG GGAATTTGGG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATATGAG GGAATTTGAG GGANTTTGGG NNNATTTGAG GGGATTTGAG GGANTTTGAG GGAATTTGAG GGAATTTGAG GGGATTTGAG GGGATTTGAG GGAATTTGAG GGAATTTGAG GGTATTTGAG GGAATTTGAG GGNNTTTGAG GGAATTTGAG GGANTTTGAG GGAATTTGAG GGAGTTTGAG GGAGTTTGAG GGAGTTTGAG GGGNTTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG GGAGTTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGANTTTGAG GGAGTTTGAG GGANTTTGAG GGAATTTGAG GGAGTTTGAG NNAATTTGAG GGAGTTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG GGAGTTTGAG GGAATTTGAG GGANTTTGAG GGAGTTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG ....|....| 75 CAGGAATGGT CAGGAATGGT CAGGAATGGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGC CGGGAATAGT CAGGAATGGT CAGGAATATT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATGGT CAGGAATAGC CAGGAATATT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATGGT CAGGAATGGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATGGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATATT CAGGAATAGT CAGGAATGGt CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CCGGAATAGT CGGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATGGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CGGGAATAGT cTGGAATAAT ....|....| 85 AGGAACATCT AGGAACATCT AGGAACATCT AGGAACATCT AGGAACATCT AGGAACATCT AGGAACATCC AGGAACATCT AGGAACATCT AGGAACATCT AGGAACATCT NGGAACATCc AGGAACATCC GGGAACATCC GGGAACATCT GGGAACATCC AGGAACATCT AGGAACATCC AGGAACATCC AGGAACATCT NGGAACATCC GGGAACATCC AGGAACATCT AGGAACATCT GGGAACATCT GGGAACATCT AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCT AGGAACATCC AGGAACATCT GGGAACATCT GGGAACATCT AGGAACATCC AGGAACATCC AGGAACATCA AGGAACATCA AGGAACATCC AGGAACATCT AGGAACATCC GGGAACATCT GGGAACATCT GGGAACATCT AGGAACATCC AGGAACATCT AGGAACATCC AGGAACATCT GGGAACATCC GGGAACATCC GGGAACATCC AGGAACATCT AGGAACATCT AGGAACATCC AGGAACATCA AGGAACATCA AGGAACATCA NGGAACATCC GGGAACATCT AGGAACATCC AGGAACATCT AGGAACATCC GGGAACATCT GGGAACATCC AGGAACATCC AGGAACATCT tGGAACTTCT 168 ....|....| 95 TTAAGAGTTT TTAAGAGTTT TTAAGAGTTT TTAAGAATCT TTAAGAATTT TTAAGAATCT CTAAGAATTT CTAAGAATTT TTAAGAGTTT TTAAGAaTTC CTAAGAATTT CTaaGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAGTTT CTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAGTTT TTAAGAGTTT CTAAGAATTT CTAAGAATTT TTAAGAATTC CTAAGAATTT CTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTC CTAAGAATTT TTAAGAGTTT TTAAGAGTTT TTAAGAATTC TTAAGAATTC TTAAGAATTC TTAAGAGTTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT CTAAGAATTT TTAAGAATTC CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT ....|....| 105 TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGAAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATCCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATCCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATCCGTAT TAATTCGTAT TAATTCGTAT TAATCCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGAAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TaATTCGCCT ....|....| 115 GGAATTAAGA GGAATTGAGA GGAATTGAGA AGAATTAGGA AGAATTGAGA GGAATTAAGA GGAATTAAGA GGAATTAAGA GGAATTGAGA AGAATTAGGA AGAACTAAGA GGAATTAAGA GGAATTAAGA AGAATTAAGA AGAACTGAGA AGAACTGAGA GGAATTGAGA GGAATTAAGA AGAATTAGGA AGAATTAAGA AGAATTAAGA AGAATTAAGA GGAATTGAGA GGAATTGAGA GGAATTAAGA GGAATTAAGA AGAATTAAGA AGAATTGAGA AGAATTGAGA AGAATTAAGA AGAACTAAGA AGAATTAAGA AGAATTAAGA GGAATTAAGA GGaATTAAGA AGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA GGAATTAAGA GGAATTGAGA GGAATTGAGA AGAATTAAGA AGAATTGGGA AGAATTAAGA GGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAGGA AGAACTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTNAGA GGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA GGAATTAAGA AGAATTAAGA AGAATTGAGA GGAATTAAGA TGAATTAGGT ....|....| 125 ACTCCAGGAT ACTCCAGGAT ACTCCAGGAT ACTCCTGGAT ACTCCTGGAT ACCCCTGGAT ACTCCTGGAT ACCCCTGGAT ACTCCAGGAT ACCCCCGGAT ATTCCCGGAT ACTCCCGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCAGGAT ACTCCTGGAT ACCCCAGGAT ACTCCCGGAT ACTCCTGGAT ACTCCTGGAT ACTCCAGGAT ACTCCAGGAT ACTCCCGGAT ACTCCCGGAT ACTCCGGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ATTCCTGGAT ACTCCTGGAT ACTCCCGGAT ACTCCCGGAT ACTCCCGGAT ACTCCTGGAT ACTCCTGGAT ACCCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCCGGAT ACTCCGGGAT ACCCCCGGAT ACTCCAGGAT ACTCCAGGAT ACTCCGGGAT ACCCCTGGAT ACTCCGGGAT ACTCCAGGAt ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACACCTGGAT ATTCCTAGAT ACCCCTGGAT ACCCCTGGAT ACCCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCCGGAT ACTCCGGGAT ACTCCCGGAT ACTCCCGGAT ACCCCAGGAT ACTCCTGGAT ACTCCTGGAT ACCCCTGGAT ACTCCAGGAT ....|....| 135 CTTTGATTGG CTTTAATTGG CTTTAATTGG CTTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CTTTAATTGG CTTTAATTGG CTTTGATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTtAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CTTTGATTGG CTTTGATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CTTTGATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTGATTGG CTTTGATTGG CCTTAATTGG CTTTAATTGG CCTTAATTGG CTTTGATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CTTTAATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CTTTAATTGG ....|....| 145 AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA GAATGATCAA AGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA aGATGATCAA AAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA GAATGATCAA GAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA GGATGATCAA AGATGATCAA AGATGATCAA GAATGATCAA GAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA GAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA TGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA GAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA GAATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA ....|....| 155 ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATa ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTANAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATCTATAATA ....|....| 165 CCATTGTTAC CCATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTtAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATTGTTAC CTATTGTAAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTATTAC CTATTATTAC CCATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CNATTGTTAC CTATTGTTAC CCATTGTTAC CCATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTtAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATCGTTAC CCATCGTTAC CTATTGTTAC CTATTGTAAC CTATTGTTAC CCATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATTGTAAC CTATTGTAAC CTATTGTTAC CtATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATCGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTAAC ....|....| 175 AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCtCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCT AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCT AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCACGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGcTCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCT AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA TGCTCATGCT AGCTCACGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCCCATGCA AGCTCACGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA TGCTCATGCT Appendix 3: Sequence data Cytochrome Oxidase I (COI) MW99520 MW99526 MW99536 MW99537 MW99538 MW99546 MW99550 MW99552 MW99559 MW99565 MW99579 MW99591 MW99605 MW99608 MW99612 MW99613 OK96022 OK99001 WE00002 WE00003 WE02321 WE02421 WE02431 WE02451 WE02491 WE02531 WE02535 WE02536 WE02591 WE02612 WE02614 WE02621 WE02661 WE02662 WE02671 WE02674 WE02676 WE02677 WE85001 WE98001 ....|....| 5 NNNNNNNNNN TCGCGAAAAT NTGCGAAAAT TCGCGAAAAT TCGCGAAAAT TAGCGAAAAT TCGCGAAAAT TAGCGAAAAT TCGCGAAAAT NNNNGAAAAT TAGCGAAAAT NNNNNNNNNN TCGCGAAAAT TCGCGAAAAT TTGCGAAAAT TCGCGAAAAT NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TAGCGAAAAT NNNNNNNAAT NNNNNNNNNN NNNNNNNNNN NNNNNNNAAT NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TAGCGAAAAT NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT NNNNNNNNNN NGAAAAAATG NNNNNNNAAT ....|....| 15 GACTTTTTTC GACTTTTTTC GACTTTTTTC GATTTTTTTC GATTTTTTTC GACTTTTTTC GATTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNNN GACTTTTTTC GACTTTTTTC GACTTTTTTC GACTTTTTTC NNNNNNNNTC NNNNNNNNNN NNNNNNNNNN GACTTTTTTC GACTTTTTTC NNNNNNNNNN NNNNNNNNNN GACTTTTTTC NNNNNNNNNC NNNNNTTTTC NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GACTTTTTTC NNNNNNNNNN NNNNNNNNNN GACTTTTTTC NNNNNNNNNN NNNNNTTTTC NNNNNNNNNN GACTTTTTTC NNNNNNNNNN ACTTTTTTTC GACTTTTTTC ....|....| 25 AACAAATCAT TACAAATCAT TACAAATCAT CACAAATCAT CACAAATCAT TACAAATCAT CACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATcAT TACAAATCAT TACAAATCAT TACAAATCAT TACAAATCAT NNNNNNNNNT NNNNAATCAT TACAAATCAT TACAAATCAT NNNNNNNNNT NNCAAATCAT NNCAAATCAT TACAAATCAT TACAAATCAT NNNNNNNNNN NNNNAATCAT NNNNNNNCAT TACAAATCAT NNNNNNGCAT NNNNNNNCAT TACAAATCAT NNNAAATCAT TACAAATCAT NNNAAATCAT TACAAATCAT NNNNNNNNNT TACAAATCAT TACAAATCAT ....|....| 35 AAAGATATTG AAAGATATTG AAAGATATTG AAAGACATTG AAAGACATTG AAAGATATTG AAAGACATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATTG AAAGATATCG NAAGATATTG AAAGATATTG AAAGATATCG AAAGATATCG AAAGATATCG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATCG AAAGATATTG AAAGATATTG AAAGATATTG ....|....| 45 GAACTTTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAAcATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAAGATTATA GAACATTATA GAACATTATA GAACATTATA GGACATTATA GAACTTTATA GAACATTATA GAACATTATA GAACATTATA GGACATTATA GAACGTTATA GAACACTATA GAACATTATA GAACACTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACACTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACATTATA GAACGTTATA GAACATTATA ....|....| 55 TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTAATTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT CTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT TTTTATTTTT ....|....| 65 GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGAG GGGATTTGAG GGAATTTGAG GGNNTTTGAG GGAATTTGAG GGAATTTGAG GGAATTTGGG GGTATTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGGG GGAATTTGAG GGAGTTTGAG GGAGTTTGAG GGAGTTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAGTTTGAG GGAATTTGAG GGAATTTGAG GGAGTTTGAG ....|....| 75 CTGGAATAAT CAGGAATAGC CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAAT CAGGAATAGT CAGGAATAGT CAGGGATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CGGGAATAGT CAGGAATAGT CAGGAATAAT CGGGAATAGT CGGGAATAGT CGGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATGGT CAGGAATAGT CGGGAATAGT CGGGAATAGT CGGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT CAGGGATAGT CAGGAATAGT CAGGAATAGT CAGGAATAGT ....|....| 85 TGGAACTTCT AGGAACATCC AGGAACATCC AGGAACATCA AGGAACATCA AGGAACATCT AGGAACATCA AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCA AGGAACATCT AGGAACATCT AGGAACATCC GGGAACATCC AGGAACATCC GGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCT AGGAACATCC AGGAACATCC GGGAACATCT GGGAACATCT AGGAACATCC AGGAACATCC AGGAACATCT AGGAACATCC GGGAACATCC GGGAACATCC GGGAACATCC AGGAACATCA AGGAACATCC AGGAACATCC AGGAACATCC AGGAACATCA AGGAACATCC GGGAACATCT AGGAACATCC AGGAACATCC ....|....| 95 CTAAGAATTC CTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TtaAGAATTC CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAATTT TTAAGAATTT CTAAGAATTT TTAAGAATTT CTAAGAATTT CTAAGAATTT CTAAGAATTT TTAAGAGTTT CTAAGAATTT TTAAGAATTC TTAAGAATTC TTAAGAATTC CTAAGAATTT CTAAGAATTT CTTAGAATTT TTAAGAATTC CTAAGAATTT CTAAGAATTT TTAAGAGTTT TAAAGAATTT CTAAGAATTT ....|....| 105 TAATTCGTCT TAATTCGTAT TAATTCGTAT TAATTCGATT TAATTCGATT TAATTCGTAT TAATTCGATT TAATTCGTAT TAATCCGTAT TAATCCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGAAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATCCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATTCGTAT TAATCCGTAT ....|....| 115 TGAGTTAGGA GGAATTAAGA AGAATTAAGA AGAGCTAAGA AGAGCTAAGA AGAATTGAGA AGAGCTAAGA AGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA GGAATTAAGA GGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA GGAATTAAGA AGAATTGAGA AGAATTGAGA AGAATTGAGA GGAATTGAGA AGAATTAAGA AGAATTGAGA AGAATTGAGA AGAATTGAGA AGAATTAAGA AGAATTGAGA AGAATTAAGA AGAATTAAGA AGAATTAAGA AGAATTGAGA GGAATTGAGA AGAATTGAGA AGAATTGAGA ....|....| 125 ACTCCAGGAT ACTCCTGGAT ACCCCTGGAT ACCCCAGGAT ACCCCAGGAT ACTCCTGGAT ACCCCAGGAT ACTCCCGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACCCCTGGAT ACCCCTGGAT ACTCCCGGAT ACCCCTGGAT ACCCCTGGAT ACTCCTGGAT ACTCCAGGAT ACTCCTGGAT ACTCCTGGAT ACTCCGGGAT ACTCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCAGGAT ACTCCTGGAT ACTCCGGGAT ACTCCGGGAT ACTCCGGGAT ACCCCTGGAT ACTCCTGGAT ACTCCTGGAT ACTCCAGGAT ACCCCTGGAT ACTCCTGGAT ACTCCAGGAT ACTCCTGGAT ACTCCCGGAT ....|....| 135 CTTTAATTGG CCTTAATTGG CTTTAATTGG CATTAATTGG CATTAATTGG CTTTAATTGG CATTAATTGG CCTTAATTGG CTTTGATTGG CTTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTAATTGG CTTTAATTGG CCTTAATTGG CTTTGATTGG CTTTGATTGG CCTTAATTGG CTTTAATTGG CTTTAATTGG CCTTAATTGG CTTTAATTGG TCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTGATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTGATTGG CCTTAATTGG CCTTAATTGG CCTTAATTGG CTTTGATTGG CCTTAATTGG CCTTAATTGG ....|....| 145 AGATGATCAA AAATGATCAA AGATGATCAA TGATGACCAA TGATGACCAA AGATGATCAA TGATGACCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AAACGATCAA AAACGATCAA AGATGATCAA GGATGATCAA AGATGATCAA GGATGATCAA GGATGATCAA AGATGATCAA GGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGATGATCAA AGACGATCAA ....|....| 155 ATTTATAATA ATTTATAATA ATTTATAATA ATTTACAATA ATTTACAATA ATTTATAATA ATTTACAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATa ATTTATAACA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAATA ATTTATAACA ATTTATAATA ....|....| 165 CTATTGTAAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTGTCAC CTATTGTTAC CTATTGTCAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATCGTTAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CCATTGTCAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTCAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CTATTGTTAC CCATCGTTAC CTATTGTTAC CTATTGTTAC ....|....| 175 AGCTCATGCT AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCTCACGCA AGCCCATGCA AGCCCACGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCACGCA AGCCCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AGCTCATGCA AGCCCATGCA AGCTCATGCA AD98001 AD98009 AD98012 AD98018 AD98024 AD98029 AD98036 DS00001 DS00005 DS01001 JC00029 JC00039 JC00040 JC00042 JC00043 JC00045 JC00046 JC00051 JC00055 JC00057 JC00061 JC00062 JC00063 JC01014 JC02001 ....|....| 185 TTTATCATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATaA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTCATTATAA TTTATTATAA TTCATTATAA TTTATTATAA TTTATTATAA TTTATTATAA ....|....| 195 TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT ....|....| 205 AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCC AGTTATACCT GGTTATACCT AGTTATACCC AGTTATACCT GGTTATACCT GGTTATACCT AGTTATACCC AGTTATACCT AGTTATACCT AGTTATACCC AGTTATACCT AGTTATACCC AGTTATACCT AGTTATACCA ....|....| 215 ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATCG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATCG ATTATAATTG ATTATAATTG ATTATaATTG ATTATAATTG ATTATAATTG ATTATAATTG ....|....| 225 GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTCGG GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GAGGATTTGG ....|....| 235 TAACTGATTA TAACTGATTG TAACTGATTG TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAATTGATTA TAATTGATTA TAACTGATTA TAATTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAATTGATTA TAACTGATTA TAATTGATTA TAACTGATTA TAACTGATTA TAATTGATTA ....|....| 245 GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA ATTCCTTTAA GTTCCCTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA ....|....| 255 TATTAGGAGC TATTAGGAGC TATTGGGAGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTGGGAGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TATTAGGGGC TGTTAGGAGC TATTAGGGGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TACTAGGAGC TATTAGGAGC ....|....| 265 ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGACATA ACCTGATATA ACCTGACATA ACCTGATATA ACCTGATATA ACCTGACATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGACATA ACCTGATATA ACCTGATATA ACCTGACATA ACCTGATATA ACCTGACATA ACCTGATATA TCCTGATATA ....|....| 275 GCCTTTCCAC GCCTTTCCTC GCCTTTCCTC GCCTTTCCAC GCCTTTCCAC GCCTTTCCCC GCCTTTCCAC GCCTTTCCTC GCCTTTCCTC GCCTTCCCCC GCCTTCCCTC GCCTTTCCTC GCCTTTCCAC GCCTTCCCTC GCCTTCCCCC GCCTTCCCCC GCCTTCCCCC GCCTTCCCTC GCTTTCCCCC GCTTTCCCTC GCCTTCCCTC GCTTTCCCTC GCCTTCCCTC GCCTTCCCCC GCATTTCCTC ....|....| 285 GATTAAATAA GATTAAATAA GATTAAATAA GACTAAATAA GACTAAATAA GATTAAATAA GATTAAATAA GTTTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GAATAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GTATAAATAA ....|....| 295 TATAAGATTC CATAAGATTT TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTC CATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTC TATGAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTT ....|....| 305 TGATTATTAC TGATTACTAC TGATTACTAC TGATTATTAC TGATTATTAC TGGTTATTAC TGATTACTAC TGATTATTGC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTGC TGATTATTAC TGATTATTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTATTAC ....|....| 315 CACCATCATT CGCCTTCATT CGCCTTCACT CACCATCATT CGCCATCATT CACCATCATT CGCCATCATT CACCATCATT CACCATCATT CACCATCATT CTCCATCATT CCCCATCCCT CACCATCATT CCCCATCATT CACCATCCTT CACCATCCTT CACCATCCTT CTCCATCATT CCCCATCATT CTCCATCATT CTCCATCATT CCCCATCATT CTCCATCATT CACCATCCTT CCCCCTCTCT ....|....| 325 AATACTACTA AATATTACTA AATACTACTA AATACTACTA AATACTACTA AATACTATTA AATATTACTA AATTCTATTA AATTCTATTA AATACTACTA GATGCTACTA AATACTACTA AATACTACTA GATGCTACTA AATACTACTA AATACTACTA AATACTACTA GATGCTACTA AATATTATTA AATATTATTA GATTCTACTA AATATTATTA GATTCTACTA AATACTACTA AATTCTATTA ....|....| 335 ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA ATTTCAAGAA ATTTCTAGAA ATTTCAAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA ....|....| 345 GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTCTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA ....|....| 355 AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGGGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGGGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGTGCA 169 Appendix 3: Sequence data JM00001 MW00001 MW00015 MW00017 MW00020 MW00024 MW00032 MW00044 MW00051 MW00056 MW00058 MW00059 MW00060 MW00064 MW00072 MW00076 MW00087 MW00101 MW00102 MW00110 MW00116 MW00119 MW00125 MW00127 MW00129 MW00140 MW00151 MW00157 MW00176 MW00177 MW00178 MW00179 MW00183 MW00185 MW00186 MW00189 MW00226 MW00229 MW00231 MW00232 MW00234 MW00259 MW00262 MW00267 MW00269 MW00290 MW00302 MW00316 MW00326 MW00327 MW00328 MW00330 MW00335 MW00347 MW00348 MW00358 MW00393 MW00409 MW00412 MW00424 MW00444 MW00452 MW00469 MW00476 MW00497 MW00498 MW00504 MW00517 ....|....| 185 TTTATTATAA TTTATTATAA TTTATTATAA TTTAtTATAA TTCATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTAtAA TTTATTATAA TTtATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATtATaA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAa TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTAtAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA ....|....| 195 TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT ....|....| 205 GGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTAAtACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCC GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCA GGTTATACCT AGTTATACCT GGTTATACCT Cytochrome Oxidase I (COI) ....|....| 215 ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATaATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATCG ATTATAATCG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAAtTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ....|....| 225 GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTCGG GAGGATTTGG GAGGATTCGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTCGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTCGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GNGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GAGGATTCGG GAGGATTTGG GAGGATTTGG GAGGATTCGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG ....|....| 235 TAACTGATTA TAACTGATTA TAACTGATTA TAATTGACTT TAATTGATTA TAATTGACTA TAACTGATTA CAATTGACTT TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAATTGATTA TAACTGATTA TAACTGATTA NAACTGATTA TAATTGATTA AAATTGACTT TAACTGATTG TAACTGATTA TAACTGATTG TAACTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAACTGATTG TAACTGATTG TAACTGATTG TAACTGATTA TAACTGATTA TAACTGATTA TAATTGATTA CAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTG TAACTGATTG TAACTGATTG TAACTGATTA TAACTGATTA TAATTGATTA TAATTGATTA TAACTGATTA TAATTGACTA TAACTGATTA TAACTGATTA TAACTGATTG TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA AAATtGATTA TAACTGATTA TAACTGATTA TAATTGATTG ....|....| 245 GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA ATTCCTTTAA GTTCCTTTAA ATTCCTTTAA GTTCCTTTAA GtCCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GtACCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA ATTCCTTTAA GTTCCTTTAA GTTCCTTTAA ATTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA ATTCCTTTAA ATTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA GTTCCTTTAA GTCCCTTTAA GTTCCTTTAA ATTCCTTTAA GTTCCATTGA ....|....| 255 TATTAGGAGC TATTAGGAGC TACTAGGAGC TATTAGGAGC TATTAGGAGC TATTGGGAGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGGGC TATTAGGGGC TATTAGGGGC TATTAGGGGC TGTTAGGAGC TATTAGGGGC TATTGGGGGC TATTGGGGGC TATTAGGAGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TATTAGGGGC TATTGGGGGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGGGC TATTGGGGGC TATTGGGGGC TATTGGGGGC TACTGGGAGC TACTGGGAGC TACTGGGAGC TACTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TACTAGGGGC TACTAGGGGC TATTAGGGGC TATTGGGAGC TATTAGGAGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTGGGAGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTGGGAGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGGGC TGTTAGGAGC TATTAGGGGC TATTAGGAGC ....|....| 265 ACCTGATATA ACCTGATATA ACCTGATATA TCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA CCCAGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA GCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTAATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA GCCTGATATA ACCTGATATA ACCTGATATA ACCAGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGACATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA TCCAGATATA ACCTGATATA ACCTGATATA ACCTGATATA 170 ....|....| 275 GCTTTTCCAC GCCTTTCCAC GCATTCCCCC GCTTTTCCAC GCTTTCCCTC GCTTTTCCTC GCCTTTCCAC GCTTTTCCAC GCCTTTCCAC GCCTTTCCAC GCCTTTCCAC GCCTTTCCAC GCCTTTCCAC GCTTTCCCAC GCCTTTCCAC GCCTTTCCTC GCCTTTCCTC GCCTTTCCAC GCCTTTCCAC GCCTTTCCAC GCTTTCCCTC GCCTTTCCAC GCCTTTCCTC GCCTTTCCAC GCCTTTCCTC GCCTTTCCAC GCCTTTCCTC GCCTTTCCAC GCTTTCCCCC GCCTTTCCTC GCCTTTCCTC GCCTTTCCTC GCATTCCCCC GCATTCCCCC GCATTCCCCC GCCTTCCCCC GCCTTTCCAC GCATTTCCAC GCATTTCCAC GCCTTTCCAC GCCTTTCCTC GCTTTTCCAC GCTTTTCCAC GCCTTTCCAC GCCTTTCCAC GCTTTTCCTC GCATTTCCTC GCCTTTCCAC GCTTTCCCAC GCCTTTCCGC GCCTTTCCAC GCCTTTCCTC GCTTTTCCAC GCCTTTCCAC GCCTTTCCAC GCCTTTCCAC GCCTTTCCGC GCTTTTCCAC GCCTTCCCTC GCCTTTCCAC GCCTTTCCAC GCCTTTCCAC GCATTCCCTC GCCTTTCCAC GCTTTCCCCC GCTTTCCCAC GCTTTTCCTC GCTTTCCCTC ....|....| 285 GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GAATAAATAA GAATAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GAATAAATAA GATTAAATAA GANTAAaTAA GATTAAaTAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAACAA GATTAAaTAA GATTAAACAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAACAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAACAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GAATAAATAA ....|....| 295 CATAAGATTT TATAAGATTC TATAAGAtTC TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTC CATAAGATTC CATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTT CATAAGATTT TATAAGATTC TATGAGATTT TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTC TATGAGATTT TATGAGATTT TATGAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTT TATAAGATTC CATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC CATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATGAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTC TATAAGaTTT TATAAGATTT TATAAGATTC TATAAGATTT ....|....| 305 TGATTATTAC TGATTATTAC TGATTACTAC TGATTATTAC TGATTATTAC TGACTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTACTCC TGATTATTAC TGATTACTCC TGATTATTAC TGATTATTAC TGATTACTCC TGATTACTCC TGATTATTAC TGATTATTAC TGATTGTTAC TGATTACTAC TGATTATTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTGTTAC TGATTATTGC TGATTATTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTACTTC TGATTACTTC TGATTACTCC TGATTATTGC TGATTATTGC TGATTACTAC TGATTATTAC TGATTACTAC TGATTACTAC TGATTATTGC TGATTACTAC TGATTACTCC TGATTACTCC TGATTACTAC TGATTACTCC TGATTACTAC TGATTACTCC TGATTATTAC TGATTATTGC TGATTACTCC TGATTACTAC TGATTATTAC TGATTACTCC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC ....|....| 315 CGCCATCATT CGCCATCATT CACCATCCTT CCCCATCTTT CCCCATCATT CACCATCATT CGCCATCATT CCCCATCTTT CACCATCATT CACCATCATT CACCATCATT CACCATCATT CACCATCATT CGCCATCATT CACCATCATT CCCCATCATT CCCCATCATT CACCATCATT CACCATCATT CACCATCATT CCCCATCTTT CTCCATCCTT CGCCTTCATT CGCCATCATT CGCCTTCACT CGCCATCATT CGCCTTCATT CGCCATCATT CCCCATCATT CGCCTTCACT CGCCTTCACT CGCCTTCACT CACCATCCTT CACCATCCTT CACCATCCTT CACCATCCTT CACCATCATT CGCCATCATT CGCCATCATT CACCATCATT CGCCTTCATT CACCATCATT CACCATCATT CACCATCATT CACCATCATT CTCCGTCATT CCCCATCATT CACCATCATT CCCCATCATT CCCCATCATT CACCATCATT CGCCTTCACT CACCATCATT CACCATCATT CACCATCATT CACCATCATT CCCCATCATT CACCATCATT CTCCATCATT CaCCATCATT CACCATCATT CACCATCATT CCCCATCATT CACCATCATT CTCCCTCATT CGCCATCATT CTCCATCATT CCCCATCATT ....|....| 325 AATACTACTA AATACTACTA AATACTACTA ATTATTGTTA AATATTATTA AATATTATTA AATACTACTA ATTATTATTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTATTA AATACTACTA AATACTATTA AATACTACTA AATACTACTA AATACTATTA AATACTATTA AATACTACTA AATATTATTA ATTATTATTA AATATTACTA AATACTACTA AATaCTATTA AATATTACTA AATATTACTA AATACTACTA AATACTACTA AATACTATTA AATACTATTA AATACTACTA AATACTACTA AATACTACTA AAtACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATATTACTA AATACTATTA AATACTATTA AATACTATTA AATATTACTA AATACTATTA AATACTACTA AATACTACTA AATATTACTA AATACTACTA AATATTACTA AATACTACTA AATACTACTA AATACTATTA AATATTACTA AATACTATTA AATACTACTA AATACTACTA GATTCTACTA AATATTACTA AATACTATTA AATATTACTA AATACTTTTA AATACTATTA AATACTTTTA AATACTACTA AATACTATTA AATATTATTA ....|....| 335 ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA ATTTCAAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTTCAAGAA ATTTCAAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCAAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCAAGAA ATTTCTAGAA ATTTCCAGAA ATTTCAAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA aTTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTAGAAGTA ATTTCTAGAA ATTTCCAGAA ATTTCAAGAA ....|....| 345 GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTTGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTCGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATTCTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAGTTGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTCTAGA GAATTGTAGA GAATTGTAGA GAATTGTaGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCCTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA ....|....| 355 AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCG AAATGGAGCA AAATGGAGCG AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCG AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGaGCa AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAaTGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGGGCA AAATGGGGCA AAATGGAGCA AAATGGAGCA AAATGGAGCG AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCG AAATGGAGCA AAATGGAGCG AAATGGGGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCG AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGTA Appendix 3: Sequence data MW00525 MW00530 MW00539 MW00547 MW01001 MW01009 MW01011 MW01014 MW01018 MW01019 MW01022 MW01023 MW01025 MW01027 MW01034 MW01039 MW01048 MW01053 MW01059 MW01061 MW01072 MW01078 MW01083 MW01092 MW01103 MW01105 MW01107 MW01114 MW01116 MW01120 MW02001 MW02006 MW02007 MW02008 MW02009 MW02021 MW02024 MW02025 MW02028 MW02031 MW02033 MW02034 MW98002 MW98008 MW98009 MW98020 MW98029 MW98049 MW98079 MW98084 MW98089 MW98094 MW98097 MW98103 MW98106 MW98128 MW98129 MW98133 MW98136 MW98138 MW98139 MW98154 MW98162 MW98170 MW98172 MW98180 MW98183 MW98185 ....|....| 185 TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATCATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTtATTATaA TTTATCATAA TTTATCATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATCATAA TTTATTATAA TTTATCATAA TTTATCATAA TTTATTATAA TTCATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATCATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATtATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA ....|....| 195 TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TCTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTCTTCAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTCTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT ....|....| 205 AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTAtACCC AGTAATACCT AGTTATACCT GGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTtATACCT AGTTATACCT GGTTATACCT AGTTATACCA AGTTATACCT AGTTATACCT AGtTATACCT AGTTATACCC AGtTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCA AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCA AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTtATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCC AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT Cytochrome Oxidase I (COI) ....|....| 215 ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATCG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATtG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATtG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ....|....| 225 GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTCGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GGGGATTTGG GAGGGTTTGG GAGGATTTGG GTGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGtTTGG GAGGGTTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GTGGATTTGG GAGGATTTGG GAGGATTTGG GTGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GGGGGTTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG ....|....| 235 TAATTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA AAACTGATTA AAATTGATTA TAACTGATTA AAATTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA tAATTGATTA CAACTGATTA AAATTGATTA TAATTGATTG TAATTGATTA TAATTGACTT AAATTGATTA TAATTGACTT AAATTGATTA TAATTGATTA AAATTGACTT AAACTGATTA AAATTGATTA tAATTGATTA TAATTGATTA AAATTGATTA TAATTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAACTGATTA TAACTGATTG TAACTGATTA TAACTGATTG TAATTGACTT TAACTGATTA TAACTGATTA TAACTGATTA TAACtGATTA TAATTGATTA TAACTGATTA TAACTGATTG TAACTGATTG TAACTGATTG TAACTGATTG TAACTGATTA TAACTGATTG TAACTGATTA TAACTGATTG TAACTGATTG TAACTGATTA ....|....| 245 GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA ATTCCTTTAA GtACCTTTAA GTTCCTTTAA GtACCTTTAA GTTCCTTTGA ATCCCATTAA GTGCCTTTAA GTTCCTTTAA GTGCCATTAA GTACCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA ATTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA GtACCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA GtACCTTTAA GTTCCACTTA GTTCCTTTAA GTTCCTTTAA ATCCCTTTAA ATTCCTTTAA GTTCCTTTAA GTTCCATTAA GTACCTTTAA GTACCTTTAA ATCCCATTAA GtACCTTTAA GTTCCTTTAA GTCCCTTTAA GTACCATTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTGA ATTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTtCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTGA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA ....|....| 255 TATTAGGAGC TATTAGGGGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TATTGGGAGC TATTAGGGGC TGTTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTGGGAGC TATTAGGAGC TATTGGGAGC TATTAGGAGC TATTGGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTGGGGGC TATTGGGGGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTGGGGGC TATTAGGTGC TATTAGGGGC TGTTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTGGGAGC TATTAGGAGC TATTAGGTGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTNGGAGC TATTAGGAGC TATTAGGNGC TACTAGGGGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TGTTAGGGGC TATTAGGAGC TATTAGGAGC TATTGGGGGC TATTAGGAGC TATTGGGGGC TATTAGGAGC TATTAGGAGC TATTGGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGGGC ....|....| 265 ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCCGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA CCCTGATATA ACCTGACATA CCCAGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA TCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACNTGATATA CCCTGATATA ACCTGATATA ACCTGATATA CCCTGATATA CCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA TCCAGATATA ACCTGATATA ACCTGATATA ACCTGATATG ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA NCCTGATATA ACCTGACATA ACCTGATATA ACCTGATATA ACCTGATATA ACCCGATATA ACCTGACATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA aCCTGATATA ACCTGATATG ACCTGATATG ACCTGATATA ACCTGATATA ACCTGATATA 171 ....|....| 275 GCTTTCCCAC GCCTTCCCTC GCCTTTCCAC GCCTTTCCAC GCTTTTCCAC GCCTTTCCTC GCATTCCCTC GCCTTCCCCC GCATTCCCTC GCCTTTCCAC GCTTTCCCAC GCTTTTCCAC GCCTTCCCTC GCTTTTCCAC GCATTCCCTC GCTTTTCCAC GCTTTTCCTC GCTTTTCCAC GCATTCCCTC GCTTTCCCTC GCCTTCCCCC GCTTTTCCAC GCATTTCCTC GCATTCCCTC GCATTCCCTC GCCTTCCCCC GCTTTTCCAC GCATTTCCTC GCATTCCCTC GCTTTCCCAC GCTTTCCCTC GCCTTTCCTC GCTTTTCCAC GCATTTCCTC GCTTTTCCTC GCATTTCCAC GCATTTCCTC GCTTTCCCTC GCTTTCCCAC GCATTTCCAC GCTTTTCCCC GCTTTCCCAC GCATTTCCTC GCATTTCCTC GCCTTCCCAC GCTTTCCCAC GCCTTTCCTC GCCTTTCCCC GCCTTTCCTC GCCTTCCCCC GCCTTTCCTC GCTTTTCCAC GCCTTTCCTC GCTTTCCCCC GCCTTTCCCC GCCTTTCCAC GCATTCCCTC GCCTTCCCTC GCCTTTCCTC GCCTTTCCTC GCCTTTCCTC GCCTTTCCTC GCCTTTCCTC GCCTTTCCtC GCTTTCCCAC GCCTTTCCTC GCCTTTCCTC GCATTCCCTC ....|....| 285 GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GAATAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAACAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GTATAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GAATAAATAA GAATAAATAA GAATAAATAA GTATAAATAA GAATAAATAA GAATAAATAA GAATAAATAA GAATAAATAA GAATAAATAA GAATAAATAA GATTAAACAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAaTAA GATTAAaTAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA ....|....| 295 TATAAGATTT TATAAGATTC CATAAGATTC TATAAGATTC CATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTT CATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTT CATAAGATTT TATAAGATTT CATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTC CATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTC CATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTT CATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTT CATAAGATTT TATAAGATTc TATAAGATTC TATAAGATTT CATAAGATTC TATAAGATTT TATAAGATTT TATAAGATTC TATAAGATTT CATAAGATTT TATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATGAGATTT TATAAGATTT TATGAGATTT TATAAGATTT TATAAGATTT TATGAGATTT TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTT ....|....| 305 TGATTATTAC TGATTATTAC TGATTATTGC TGATTACTCC TGATTATTAC TGATTATTGC TGATTATTAC TGATTACTAC TGATTATTAC TGATTATTGC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTATTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTGTTAC TGATTAcTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTATTAC TGATTACTAC TGATTATTAC TGGTTATTAC tGATTATTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTGCTAC TGATTACTAC TGATTGCTAC TGATTACTAC TGATTACTAC TGATTATTAC TGATTGCTAC TGATTGCTAC TGATTATTAC ....|....| 315 CCCCATCATT CCCCATCATT CGCCATCATT CACCATCATT CGCCATCATT CCCCATCCCT CCCCATCATT CACCATCCTT CTCCATCATT CCCCATCATT CTCCGTCATT CTCCATCCCT CTCCATCATT CACCCTCACT CTCCATCATT CGCCATCATT CTCCATCATT CGCCATCATT CCCCATCATT CCCCATCATT CACCATCCTT CGCCATCATT CTCCATCATT CCCCATCATT CTCCATCATT CACCATCCTT CGCCATCATT CCCCCTCTCT CCCCATCATT CCCCATCTTT CCCCATCATT CCCCATCACT CCCCATCCTT CTCCATCTTT CTCCATCTTT CTCCATCCTT CTCCCTCTTT CACCATCATT CTCCGTCATT CTCCATCATT CCCCATCATT CCCCATCATT CACCATCATT CCCCATCATT CACCATCATT CCCCATCATT CTCCATCATT CACCATCATT CACCTTCATT CACCATCCTT CACCTTCaTT CNCCATCATT CGCCTTCATT CCCCATCATT CACCATCATT CGCCATCATT CCCCATCATT CTCCATCATT CGCCTTCACT CACCTTCATT CGCCTTCACT CACCTTCATT CGCCTTCATT CGCCTTCACT CTCCATCATT CACCTTCATT CACCTTCATT CCCCATCATT ....|....| 325 AATATTATTA GATACTACTA AATATTGCTA AATACTATTA AATACTACTA AATACTATTA AATACTTTTA AATACTACTA AATACTTTTA AATACTTCTA AATATTACTA TATATTATTA GATTCTACTA AATATTATTA AATACTTTTA AATACTACTA AATATTATTA AATACTACTA AATACTTTTA AATATTATTA AATACTACTA AATACTACTA AATACTGCTA AATACTTTTA AATACTTTTA AATACTATTA AATACTACTA AATTCTATTA AATACTTTTA AATATTATTA AATACTACTG AATACTACTA ATTATTATTA GATACTGTTA ATTATTATTA AATATTATTA AATTCTCCTA AATATTATTA AATATTGCTA AATATTATTA AATATTATTA AATATTATTA AATACTATTA AATACTACTA AATACTACTA AATATTACTA AATACTACTA AATACTATTA AATATTACTA AATACTACTA AATATTACTA ATTATTATTA AATATTACTA AATACTACTA AATACTATTA AATACTTCTA AATACTTTTA GATTCTACTA AATACTACTA AATATTACTA AATACTACTA AATATTACTA AATATTACTA AATACTACTA AATATTACTA AATATTACTA AATATTACTA AATACTTTTA ....|....| 335 ATTTCTAGAA ATTTCTAGAA ATCTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA ATTTCAAGAA ATTTCTAGAA ATTAGAAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA ATTTCTAGAA ATTTCAAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA ATTTCAAGAA ATTTCAAGAA ATTTCTAGAA ATTTCAAGAA ATTTCTAGAA ATTTCAAGAA ATTTCAAGTA ATTTCAAGAA ATTTCTAGAA ATTTCAAGAA ATTTCTAGAA ATTTCCAGAA ATTTCTAGAA ATTTCAAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGaA ATTTCTAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ....|....| 345 GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTTGA GAATTGTAGA GAATTGTTGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATCTTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGa GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA ....|....| 355 AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCG AAATGGAGCA AAATGGAGCA AAATGGAGCG AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGTGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGTGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAACGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGGGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA Appendix 3: Sequence data MW98189 MW98203 MW98205 MW98212 MW98220 MW98228 MW98230 MW98235 MW98240 MW98261 MW98264 MW98265 MW98270 MW98274 MW98278 MW98284 MW98285 MW98294 MW98313 MW98315 MW99001 MW99006 MW99009 MW99013 MW99014 MW99018 MW99028 MW99038 MW99042 MW99045 MW99047 MW99053 MW99057 MW99058 MW99059 MW99060 MW99061 MW99068 MW99080 MW99084 MW99094 MW99095 MW99097 MW99102 MW99104 MW99105 MW99124 MW99128 MW99134 MW99135 MW99140 MW99141 MW99158 MW99163 MW99164 MW99165 MW99170 MW99174 MW99187 MW99196 MW99203 MW99221 MW99226 MW99240 MW99247 MW99263 MW99264 MW99274 ....|....| 185 TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTCATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATCATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATCATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA ....|....| 195 TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTCAT TTTTTTTTAT TTTTTTTTAT TTTTTTTCAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTCAT TTTTTTTTAT TTTTTTTTAT TTTTTtTTAT TTTTTTTCAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTCTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT ....|....| 205 AGTTATACCC AGTTATACCT GGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCA AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTAATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT Cytochrome Oxidase I (COI) ....|....| 215 ATTATAATTG ATTATAATTG ATCATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ....|....| 225 GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGaTTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GTGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGaTTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GTGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GaGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GGGGNTTTGG GAGGATTTGG GAGGATTTGG GGGGATTTGG GGGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GGGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GNGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GNGGATTTGG GGGGATTTGG GAGGATTTGG GTGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GGGGATTTGG GGGGATTTGG GAGGATTTGG ....|....| 235 TAACTGATTG TAACTGATTG TAACTGATTA TAACTGATTA TAATTGACTA TAACTGATTA AAATTGACTT TAACTGATTA TAATTGATTA TAACTGATTA TAACTGATTA AAACTGATTA TAATTGATTA TAATTGATTA TAACTGATTA TAACTGATTA TAACTGATTG TAACTGATTA TAACTGATTG TAACTGATTA TAATTGATTA TAACTGATTG TAACTGATTA TAATTGATTA TAACTGATTA TAATTGATTA TAATTGATTA TAACTGATTA TAATTGATTA AAATTGATTA CAACTGATTA TAACTGATTA TAACTGATTG TAACTGATTG TAACTGATTG TAACTGATTA TAACTGATTG TAATTGATTA AAATTGACTA AAATTGATTA TAACTGATTG TAACTGATTA TAATTGATTA TAATTGATTA TAATTGATTA TAATTGATTA TAATTGATTA TAATTGATTA TAATTGATTA TAACTGATTA TAACTGATTA TAATTGATTA AAATTGATTA TAATTGATTA TAACTGATTA TAACTGATTG TAACTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAACTGATTA AAATTGACTA TAACTGATTA TAACTGATTG TAACTGATTA TAATTGATTA TAATTGATTA TAACTGATTA ....|....| 245 GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA ATCCCTTTAA GTTCCCTTAA GTTCCTTTAA GTTCCTTTAA GTCCCCTTAA ATCCCATTAA GTCCCTTTAA GTTCCTTTAA GTACCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTCCCCTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTtTAA GTTCCCTTAA GTACCTTTAA GTACCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA ATTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA GTTCCTTTAA GTACCACTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTCCCTTTAA GTACCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA ....|....| 255 TATTAGGAGC TATTGGGGGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TGTTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTGGGAGC TATTAGGAGC TATTAGGAGC TATTGGGGGC TATTGGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TGTTAGGGGC TATTAGGAGC TATTAGGAGC TATTGGGGGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGGGC TATTGGGGGC TATTAGGAGC TATTGGGGGC TATTAGGAGC TATTAGGAGC TACTAGGNGC TATTAGGAGC TATTAGGAGC TATTGGGGGC TATTAGGGGC TATTAGGGGC TACTAGGAGC TACTAGGAGC TACTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TACTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTGGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TACTAGGAGC TATTAGGGGC TATTAGGAGC TATTAGGGGC TATTGGGGGC TATTAGGAGC TACTAGGAGC TACTAGGAGC TATTAGGGGC ....|....| 265 ACCTGATATA ACCTGATATG ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCAGATATA ACCTGATATA ACCTGATATA ACCAGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATa ACCAGATATA ACCTGATATA TCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATG ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA aCCTGATATa ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA TCCAGATATA ACCTGATATA CCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCAGATATA ACCTGATATA ACCTGATATA ACCTGATATG ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA TCCTGATATA ACCTGATATA ACCTGATATG ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA 172 ....|....| 275 GCCTTTCCTC GCCTTTCCTC GCCTTTCCAC GCCTTTCCAC GCTTTTCCTC GCATTCCCTC GCTTTTCCAC GCTTTCCCCC GCCTTTCCAC GCCTTTCCAC GCTTTCCCCC GCTTTCCCAC GCTTTCCCTC GCCTTTCCTC GCATTCCCTC GCCTTTCCTC GCCTTTCCTC GCCTTTCCAC GCCTTTCCTC GCCTTTCCAC GCTTTCCCTC GCCTTTCCTC GCCTTTCCTC GCTTTCCCTC GCCTTTCCAC GCTTTCCCAC GCTCTCCCTC GCTTTCCCCC GCATTTCCTC GCTTTTCCAC GCTTTCCCTC GCCTTTCCAC GCCTTTCCTC GCCTTTCCTC GCCTTTCCTC GCCTTTCCAC GCCTTTCCTC gCCTTCCCCC GCATTTCCAC GCATTtCCTC GCCTTTCCTC GCCTTTCCAC GCTTTCCCTC GCCTTCCCCC GCCTTCCCCC GCCTTCCCCC GCTTTCCCTC GCATTTCCTC GCCTTCCCTC GCCTTTCCCC GCATTCCCTC GCCTTCCCCC GCTTTTCCTC GCTTTTCCCC GCCTTTCCAC GCCTTTCCTC GCCTTTCCTC GCCTTTCCAC GCCTTTCCAC GCCTTCCCCC GCCTTTCCCC GCTTTTCCAC GCCTTTCCAC GCCTTTCCtC GCCTTTCCTC GCCTTCCCCC GCCTTCCCCC GCATTTCCAC ....|....| 285 GATTAAATAA GATTAAATAA GACTAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GAATaAATAA GATTAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GACTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GAATAAATAA GATTAAATAA GACTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GACTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA ....|....| 295 TATAAGATTT TATGAGATTT TATAAGATTT TATAaGATTT TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTT CATAAGATTC TATAAGATTT TATAAGATTT CATAAGATTT TATAAGATTC TATGAGATTT TATAAGATTT TATGAGATTT CATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTT TATAAGATTC TATAAgATTC CATAAGATTT CATAAGATTT TATAAGATTC TATGAGATTT CATAAGATTT TATGAGATTT TATAAGATTC TATAAGATTT TATAAGATTC CATAAGATTT TATAAGATTT TATGAGATTT TATAAGATTC TATGAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTC CATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTT TATAAGATTT TATGAGATTT TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTC TATGAGATTT TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTT ....|....| 305 TGATTACTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTGTTAC TGATTATTAC TGACTATTAC TGATTACTAC TGATTATTAC TGACTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTACTAC TGGTTATTAC TGATTATTAC TGATTATtAC TGATTATTAC TGAttATTAC TGACTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTATTAC TGATTACTAC TGATTATTAC TGATTGCTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTGCTAC TGATTGCTAC TGATTATTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTGCTAC TGATTATTAC TGATTATTAC TGATTATTaC TGATTACTAC TGGTTATTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTATTAC ....|....| 315 CACCTTCATT CGCCTTCACT CACCATCATT CGCCATCATT CACCATCATT CCCCATCACT CTCCATCTCT CCCCATCATT CACCATCATT CACCATCATT CCCCATCATT CTCCGTCATT CTCCATCATT CCCCATCATT CCCCATCATT CGCCTTCATT CGCCTTCATT CACCATCATT CGCCTTCACT CGCCATCATT CCCCATCATT CGCCTTCATT CGCCATCATT CCCCATCTTT CACCATCATT CACCATCACT CCCCATCATT CTCCATCATT CTCCATCATT CACCTTCATT CCCCATCATT CACCATCATT CACCTTCACT CGCCTTCATT CGCCTTCACT CACCATCATT CACCTTCATT CACCATCCTT CTCCATCATT CTCCATCTTT CGCCTTCACT CGCCATCATT CTCCATCATT CGCCATCCTT CACCATCCTT CACCATCCTT CCCCATCTTT CCCCATCATT CCCCATCATT CTCCATCATT CCCCATCATT CACCATCCTT CTCCTTCATT CCCCATCATT CACCATCATT CGCCTTCACT CGCCATCATT CGCCATCATT CGCCATCATT CACCATCCTT CTCCATCATT CCCCTTCATT CACCATCATT CGCCTTCACT CGCCTTCATT CACCATCCTT CACCATCCTT CACCATCATT ....|....| 325 AATATTGCTA AATACTACTA AATACTACTA AATACTACTA AATATTGTTA AATACTTTTA ATTATTATTA AATATTATTA AATACTACTA AATACTATTA AATATTATTA AATATTACTA AATATTATTA AATGCTACTA AATACTTTTA AATATTACTA AATATTACTA AATACTACTA AATACTACTA AATACTACTA AATATTATTA AATATTACTA AATACTATTA AATATTACTA AATACTTCTA AATATTATTA AaTATTATTA AATATTATTA AATACTTTTA AATATTATTA AATACTACTA AATACTACTA AATACTACTA AATATTACTA AATACTACTA AATACTACTA AATATTACTA AATACTACTA AATATTATTA GATATTGTTA AATATTACTA AATACTACTA AATATTATTA AATACTACTA AATACTACTA AATACTACTA AATATTATTA AATACTACTA AATATTATTA AATACTACTA AATACTTTTA AATACTACTA AATATTATTA AATATTATTA AATACTACTA AATACTACTA AATACTATTA AATATTACTA AATATTACTA AATACTACTA AATACTACTA AATATTATTA AATACTACTA AATACTACTA AATATTACTA AATACTACTA AATACTACTA AATACTACTA ....|....| 335 ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGTA ATTTCTAGAA ATTTCAAGAA ATTTCAAGAA ATTTCCAGAA ATTTCCAGAA ATTTCAAGAA ATTTCAAGAA ATTTCAAGAA ATTTCAAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCAAGAA ATTTCCAGAA ATTTCTAGAA ATTTCAAGAA ATTTCTAGGA ATTTCTAGAA ATTTCAAGAA ATTTCAAGAA ATTTCtAGAA ATTTCAAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCAAGTA ATTTCAAGAA ATTTCCAGAA ATTTCCAGAA ATTTCAAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCAAGAA GTTTCAAGAA ATTTCtAGAA ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ....|....| 345 GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTTGA GAATTGTAGA GAATTGTAGA GGATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATtGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GTATTGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATTCTAGA GAATTCTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA ....|....| 355 AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAgCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AACTGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGGGCA AAATGGGGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA Appendix 3: Sequence data MW99276 MW99286 MW99289 MW99292 MW99301 MW99303 MW99309 MW99314 MW99319 MW99320 MW99341 MW99344 MW99353 MW99357 MW99372 MW99374 MW99376 MW99380 MW99381 MW99382 MW99393 MW99398 MW99406 MW99407 MW99408 MW99413 MW99422 MW99425 MW99430 MW99435 MW99448 MW99449 MW99465 MW99469 MW99471 MW99473 MW99476 MW99479 MW99494 MW99501 MW99508 MW99514 MW99515 MW99520 MW99526 MW99536 MW99537 MW99538 MW99546 MW99550 MW99552 MW99559 MW99565 MW99579 MW99591 MW99605 MW99608 MW99612 MW99613 OK96022 OK99001 WE00002 WE00003 WE02321 WE02421 WE02431 WE02451 WE02491 ....|....| 185 TTTATTATAA TTTATTATAA TTTATCATAA TTTATTATAA TTTATTATAA TTTATCATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TtTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATCATAA TTCATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA ....|....| 195 TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTCTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTCAT TTTTTTTCAT TTTTTTTCAT TTTTTTTCAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTCAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT ....|....| 205 AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCC GGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT Cytochrome Oxidase I (COI) ....|....| 215 ATTATAATTG ATtATAATTG ATTATAATCG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATCG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATCG ATTATAATCG ATTATAATTG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ....|....| 225 GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GNGGATTTGG GAGGATTTGG GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGaTTTGG GAGGATTTGG GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGGTTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGGTTTGG GAGGATTTGG GAGGGTTTGG GAGGGTTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG ....|....| 235 TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAATTGATTG TAATTGATTG TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTG TAACTGATTG TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA AAATTGATTA TAACTGATTA TAATTGATTA TAACTGATTG TAACTGATTA TAACTGATTA AAATTGATTA TAATTGACTA TAACTGATTA TAACTGATTG TAACTGATTG TAACTGATTG TAATTGATTG TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTG TAACTGATTA TAACTGATTA AAATTGACTT TAATTGACTT TAACTGATTA TAATTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTA CAACTGATTA TAACTGATTA TAACTGATTA TAATTGATTA TAACTGATTG TAACTGATTG TAACTGATTA TAATTGATTA TAACTGATTA ....|....| 245 GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA ATTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCATTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA ATTCCTTTAA GTACCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA ATTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTGA GTTCCTTTAA GTTCCTTTAA GTACCTTTAA GTCCCCTTAA GTACCTTTAA GTTCCTTTAA GTACCTTTAA GTTCCCTTAA GTTCCCTTAA GTTCCTTTAA GTTCCCTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCCTTAA GTTCCCTTAA GTACCTTTAA GTACCTTTAA GTTCCTTTAA GTACCTTTAA GTACCTTTAA GTTCCTTTAA GTTCCTCTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA ....|....| 255 TATTAGGGGC TATTGGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTGGGGGC TATTGGGGGC TATTAGGAGC TATTAGGGGC TATTGGGGGC TATTAGGGGC TACTGGGAGC TACTGGGAGC TATTGGGGGC TATTNGGAGC TATTGGGGGC TACTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTGGGAGC TATTGGGAGC TATTAGGAGC TATTGGGGGC TATTGGGGGC TATTGGGGGC TATTAGGAGC TATTAGGGGC TATTGGGGGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TATTAGGAGC TATTAGGAGC TATTAGGGGC TATTAGGAGC TATTAGGGGC TATTGGGAGC TATTAGGAGC TATTAGGGGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TATTGGGGGC TATTAGGGGC TATTGGGAGC TATTGGGGGC TATTAGGAGC TATTAGGGGC TATTGGGGGC TATTAGGGGC TACTAGGAGC TATTAGGAGC TATTAGGGGC ....|....| 265 ACCTGATATA GCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCCGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA GCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA GCCTGATATA TCCAGATATA GCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA CCCTGATATA aCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA GCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATG ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA CCCCGATATA ACCTGATATA ACCTGATATA ACCAGATATA ACCAGATATA ACCTGATATA ACCAGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGACATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATG ACCTGATATA ACCTGATATA ACCTGATATA 173 ....|....| 275 GCATTTCCAC GCCTTTCCAC GCCTTTCCAC GCCTTTCCAC GCCTTTCCCC GCTTTCCCCC GCTTTCCCCC GCCTTTCCAC GCATTTCCAC GCATTTCCAC GCCTTTCCAC GCTTTCCCCC GCCTTTCCTC GCCTTTCCTC GCTTTCCCCC GCCTTTCCAC GCCTTTCCAC GCATTTCCAC GCATTCCCCC GCATTCCCCC GCCTTTCCAC GCTTTTCCTC GCCTTTCCAC GCCTTCCCCC GCCTTTCCTC GCCTTTCCTC GCCTTTCCTC GCTTTCCCTC GCTTTTCCtC GCATTCCCTC GCCTTTCCTC GCCTTTCCTC GCCTTTCCTC GCCTTTCCCC GCATTTCCAC GCCTTTCCAC GCCTTTCCAC GCCTTTCCAC GCTTTCCCAC GCCTTTCCTC GCCTTTCCAC GCATTCCCTC GCTTTTCCAC GCTTTTCCAC GCCTTTCCCC GCATTTCCTC GCTTTCCCCC GCTTTCCCCC GCCTTTCCAC GCTTTCCCCC GCCTTTCCAC GCTTTCCCCC GCTTTCCCCC GCCTTCCCTC GCCTTTCCAC GCCTTTCCAC GCATTCCCTC GCATTCCCTC GCCTTTCCAC GCATTCCCTC GCATTCCCTC GCCTTTCCAC GCTTTCCCCC GCTTTTCCTC GCCTTTCCTC GCCTTCCCCC GCCTTTCCAC GCCTTCCCAC ....|....| 285 GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GACTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GACTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GAATAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GACTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GACTAAATAA GATTAAATAA GAATAAATAA GAATAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GACTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA ....|....| 295 TATAAGATTT TATAAGAtTC TATAAGATTC TATAAGATTC TATAAGATTC CATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTT TATAaGATTT TATAAGATTC TATAAGATTC TATGAGATTT TATGAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTT TATAAGATTT CATAAGATTT TATAAGATTT TATAAGATTT TATGAGATTT TATGAGATTT TATGAGATTT TATAAGATTC TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTT TATAAGATTC TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTT TATAAGATTT TATAAGATTT TATAAGATTC TATAAGAtTC TATAAGATTT TATAAGATTT TATAAGATTC TATAAGATTT TATGAGATTT TATGAGATTT TATAAGATTC TATAAGATTC TATAAGATTC ....|....| 305 TGATTATTAC TGATTATTGC TGATTATTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTaC TGATTATTAC TGATTACTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTATTGC TGATTATTAC TGATTACTAC TGATTACTAC TGATTATTGC TGATTATtAC TGATTATTGC TGATTACTAC TGATTGCTAC TGATTACTAC TGATTACTAC TGATTATTAC TGACTATTAC TGATTATTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTATTAC TGATTATTAC TGATTATTGC TGATTATTAC TGATTATTAC TGATTATTAC TGATTGCTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGACTATTAC TGACTATTAC TGATTATTAC TGACTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTATTAC TGATTACTAC TGATTATTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTACTAC TGATTACTAC ....|....| 315 CACCATCATT CACCATCATT CACCATCATT CACCATCATT CCCCATCATT CACCATCATT CTCCATCATT CACCATCATT CACCATCATT CACCATCATT CACCATCATT CCCCATCATT CGCCTTCACT CGCCTTCACT CCCCATCATT CACCATCATT CACCATCATT CACCATCATT CACCATCCTT CACCATCCTT CACCATCATT CTCCTTCACT CACCATCATT CACCATCCTT CACCTTCATT CGCCTTCATT CGCCTTCATT CCCCATCATT CACCATCATT CCCCATCATT CGCCTTCACT CGCCTTCACT CGCCTTCACT CCCCATCATT CACCATCATT CACCATCATT CACCATCATT CGCCATCATT CTCCATCATT CACCTTCATT CGCCATCATT CCCCATCATT CCCCATCTTT CTCCATCTTT CTCCATCATT CTCCATCATT CCCCATCATT CCCCATCATT CCCCATCATT CCCCATCATT CGCCATCATT CTCCATCATT CTCCATCATT CCCCATCATT CGCCATCATT CGCCATCATT CCCCATCATT CTCCATCATT CCCCATCATT CTCCATCATT CTCCATCATT CACCTTCATT CCCCATCTTT CGCCTTCACT CGCCTTCACT CACCATCCTT CCCCATCATT CCCCATCATT ....|....| 325 AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATATTATTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTATTA AATACTACTA AATACTATTA AATACTATTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATATTATTA AATACTACTA AATACTACTA AATATTACTA AATATTACTA AATATTACTA AATTTTATTA AATATTATTA AATACTTTTA AATACTACTA AATACTACTA AATACTATTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATATTACTA AATATTACTA AATACTACTA AATACTTTTA ATTATTATTA ATTATTGTTA AATACTACTA AATACTTTTA AATATTATTA AATATTATTA AATACTTCTA AATATTATTA AATACTACTA AATACTACTA AATACTACTA GATGCTACTA AATACTACTA AATACTACTA AATACTTTTA AATACTTTTA AATACTTCTA AATACTTTTA AATACTTTTA AATACTaCTA AATACTGTTA AATACTACTA AATACTACTA AATACTACTA AATATTATTA AATACTACTA ....|....| 335 ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCCAGAA ATTTCAAGAA ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCAAGAA ATTTCtAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCAAGAA ATTTCAAGAA ATTTCCAGAA ATTTCTAGAA ATTTCAAGAA ATTTCAAGAA ATTTCTAGAA ATTTCAAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCTAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCCAGAA ATTTCAAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGGA ATTTCCAGAA ATTTCCAGAA ....|....| 345 GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GTATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATTGTCGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAGTTGTAGA GAATCGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA ....|....| 355 AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCa AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAGTGGAGCA AAATGGAGCA AAACGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA Appendix 3: Sequence data Cytochrome Oxidase I (COI) WE02531 WE02535 WE02536 WE02591 WE02612 WE02614 WE02621 WE02661 WE02662 WE02671 WE02674 WE02676 WE02677 WE85001 WE98001 ....|....| 185 TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA TTTATTATAA ....|....| 195 TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT TTTTTTTTAT ....|....| 205 AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT GGTTATACCT AGTTATACCT AGTTATACCT AGTTATACCT ....|....| 215 ATTATAATCG ATTATAATTG ATCATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATCG ATTATAATCG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ATTATAATTG ....|....| 225 GAGGATTTGG GGGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG GAGGATTTGG ....|....| 235 TAACTGATTA TAATTGATTA TAACTGATTG TAACTGATTA TAACTGATTA TAACTGATTA TAACTGATTG TAACTGATTA TAATTGATTA TAACTGATTA TAACTGATTG TAACTGATTA TAACTGATTA TAACTGATTA TAATTGATTA ....|....| 245 GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA GTTCCTTTAA ....|....| 255 TATTAGGGGC TACTGGGAGC TATTGGGAGC TATTGGGGGC TATTGGGGGC TATTGGGGGC TATTGGGGGC TATTAGGGGC TATTAGGGGC TATTAGGAGC TATTGGGGGC TATTAGGAGC TACTGGGAGC TATTAGGGGC TATTAGGAGC ....|....| 265 ACCTGATATA ACCTGATATA ACCTGATATG ACCTGATATA GCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATG GCCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ACCTGATATA ....|....| 275 GCCTTTCCAC GCCTTCCCTC GCCTTTCCTC GCCTTTCCAC GCCTTTCCAC GCCTTTCCAC GCCTTTCCTC GCCTTTCCAC GCTTTCCCCC GCCTTTCCAC GCCTTTCCTC GCCTTTCCAC GCATTCCCCC GCCTTCCCAC GCCTTCCCCC ....|....| 285 GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA GATTAAATAA ....|....| 295 TATAAGATTC TATAAGATTC TATGAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATGAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATGAGATTT TATAAGATTC TATAAGATTC TATAAGATTC TATAAGATTC ....|....| 305 TGATTATTAC TGATTACTTC TGATTACTAC TGATTATTGC TGATTATTGC TGATTATTGC TGATTACTAC TGATTATTAC TGATTATTAC TGATTATTGC TGATTACTAC TGATTATTAC TGATTACTAC TGATTACTAC TGATTATTAC ....|....| 315 CGCCATCATT CACCATCCTT CGCCTTCACT CGCCATCATT CGCCATCATT CGCCATCATT CACCTTCACT CGCCATCATT CCCCATCATT CACCATCATT CGCCTTCACT CACCATCATT CACCATCCTT CCCCATCATT CACCATCATT ....|....| 325 AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTACTA AATACTATTA AATACTACTA AATACTACTA AATACTACTA AATACTATTA ....|....| 335 ATTTCCAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCCAGAA ATTTCTAGAA ATTTCTAGAA ATTTCCAGAA ATTTCCAGAA ....|....| 345 GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATCGTAGA GAATTGTAGA GAATTGTAGA ....|....| 355 AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AAATGGAGCA AD98001 AD98009 AD98012 AD98018 AD98024 AD98029 AD98036 DS00001 DS00005 DS01001 JC00029 JC00039 JC00040 JC00042 JC00043 JC00045 JC00046 JC00051 JC00055 JC00057 JC00061 JC00062 JC00063 JC01014 JC02001 JM00001 MW00001 MW00015 MW00017 MW00020 MW00024 MW00032 MW00044 MW00051 MW00056 MW00058 MW00059 MW00060 MW00064 MW00072 MW00076 MW00087 MW00101 MW00102 MW00110 MW00116 MW00119 MW00125 MW00127 MW00129 ....|....| 365 GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGGACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGGT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGGACAGGAT GGAACTGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT ....|....| 375 GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTCTA GAACAGTCTA GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACGGTTTA GAACGGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA ....|....| 385 CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTA CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCaCTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCGCTT CCCCCCGCTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT ....|....| 395 TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA ....|....| 405 TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCACACAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCACATAG TTGCACATGG TTGCACACAG TTGCACATGG TTGCACATGG TTGCACATGG TTGCACACAG TTGCTCATGG TTGCTCATGG TTGCACACAG TTGCTCATGG TTGCACACAG TTGCACATGG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATGG TTGCTCATAG TTGCTCATGG TTGCACACAG TTGCACATAG TTGCTCATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCACATAG TTGCACACAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCACACAG TTGCACATAG TTGCACACAG TTGCCCATAG TTGCACATAG TTGCNCATAG TTGCACATAG ....|....| 415 AGGATCATCT AGGATCATCT AGGATCGTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCTTCT AGGATCATCT GGGATCATCT AGGATCTTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCTTCT AGGATCATCT AGGATCATCT AGGATCTTCT AGGATCATCT AGGATCTTCT AGGATCATCT AGGAAGATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGGTCATCT AGGATCATCT AGGATCTTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGGTCATCT AGGGTCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCGTCT AGGATCATCT AGGaTCATCT aGGaTCATCT ....|....| 425 GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTGGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATCTTG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAA GTAGATTTAG GTTGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGACTTAG GTAGACTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTaG ....|....| 435 CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTTTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CTATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CTATTTTTTC CAATTTTTTC CAATTTTTTC CAATTTTCTC CTATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CTATTTTTTC CAATTTTCTC CaATTtTCTC CAATTTTTTC ....|....| 445 CCTTCACTTA CCTTCATTTG TCTTCATTTG TCTTCATTTA TCTTCATTTA TCTTCACTTA CCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTCCATTTA TCTTCATTTA TCTTCATTTA ACTTCATTTA ACTTCATTTA ACTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA ACTTCATTTG ACTTCACTTA TCTTCATTTA TCTTCATTTA ACTTCATTTA TTTACATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TTTACATTTA GCTTCATTTA GCTTCATTTA GCTTCATTTA GCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA ACTTCATTTA ACTTCATTTA TCTTCATTTA TCTTCATTTA GCTTCATTTA ACTTCATTTA TTTACATTTA CCTTCATTTA GCTTCATTTA TCTTCATTTG ....|....| 455 GCAGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCTGGAATTT GCTGGAATTT GCAGGAATTT GCTGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCTGGAATTT GCTGGAATCT GCAGGAATCT GCTGGGATTT GCAGGAATCT GCTGGGATTT GCAGGAATTT GCTGGTATTT GCAGGaATTT GCAGGAATTT GCGGGAATTT GCAGGTATTT GCAGGAATCT GCTGGAATTT GCAGGAATTT GCAGGTATCT GCCGGAATTT GCCGGAATTT GCCGGAATTT GCCGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCTGGAATTT GCTGGAATTT GCAGGAATTT GCAGGAATTT GCCGGAATTT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCCGGAaTTT GCGGGAATTT ....|....| 465 CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CATCTATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CCTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTNT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT ....|....| 475 AGGAGCaATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGGGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGGGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCaATT AGGAGCAATT AGGTGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGGGCAATT AGGGGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT ....|....| 485 AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTCATTA AATTTTATTa AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTaTTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA ....|....| 495 CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACtATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATCAT CAACTATTAT CAACAATTAT CAACCATCAT CTACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CTACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT ....|....| 505 TAATATACGA CAATATACGG CAATATACGG TAATATACGG TAATATACGA tAATATACGA TAATATACGA TAATATACGG TAATATACGG TAACATACGG TAATATACGA TAATATACGA TAATATACGA TAATATACGA CAATATACGA CAATATACGA CAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATAtACGA CAATATACGA TAATATACGA TAATATACGA TAATATACGG CAATATGCGA TAATATACGA TAATATACGA TAATATGCGA TAATATACGG TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATAtGCGG TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA CAATATACGG TAATATACGA CAATATACGA ....|....| 515 GTAAATAATT GTAAATAAAT GTAAATAACC GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT ATTAATAATA GTAAATAATT GTAAATAATT GTAAATAATT ATTAATAATT GTAAATAATT GTAAACAATA GTAAATAATT ATTAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACT GTAAATAATT GTAAATAACT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACT GTAAATAATT GTAAATAATT ATTAATAATT GTAAATAAAT GTAAATAATT GTAAATAACC ....|....| 525 TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TAACCTTTGA TATCTTTTGA TATCTTTTGA TATCATTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCGTTTGA TATCATTTGA TATCCTTTGA TATCaTTTGA TATCCTTTGA TATCTTTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTga TATCTTTTGA ....|....| 535 TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATAtCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATACCA TCAAATATCA TCAAATATCA TCAAATATCC TCAAATATCC TCAAATATCC TCAAATATCA CCAAATATCA CCAAATATCA TCAAATATCA CCAAATATCA TCAAATATCA TCAAATATCC TCAAATACCT TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCT CCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA CCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA 174 Appendix 3: Sequence data MW00140 MW00151 MW00157 MW00176 MW00177 MW00178 MW00179 MW00183 MW00185 MW00186 MW00189 MW00226 MW00229 MW00231 MW00232 MW00234 MW00259 MW00262 MW00267 MW00269 MW00290 MW00302 MW00316 MW00326 MW00327 MW00328 MW00330 MW00335 MW00347 MW00348 MW00358 MW00393 MW00409 MW00412 MW00424 MW00444 MW00452 MW00469 MW00476 MW00497 MW00498 MW00504 MW00517 MW00525 MW00530 MW00539 MW00547 MW01001 MW01009 MW01011 MW01014 MW01018 MW01019 MW01022 MW01023 MW01025 MW01027 MW01034 MW01039 MW01048 MW01053 MW01059 MW01061 MW01072 MW01078 MW01083 MW01092 MW01103 ....|....| 365 GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGGT GGAACAGGGT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACGGGAT GGAACGGGAT GGAACAGGAT GGAACAGGAT GGAACAGGGT GGGACAGGAT GGAACAGGAT GGGACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACGGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGaACAGGAT GGAACAGGAT GGAACGGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACTGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT ....|....| 375 GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTATA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTGTA GAACAGTGTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA ....|....| 385 CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCNCCAcTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTA TCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT Cytochrome Oxidase I (COI) ....|....| 395 TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAACA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCCAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCCAATA TCATCTAATA TCATCTAATA ....|....| 405 TTGCACACAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATGG TTGCACATGG TTGCACATGG TTGCACATGG TTGCACATAG TCGCACATAG TCGCACATAG TTGCACATAG TTGCACATAG TTGCGCACAG TTGCGCACAG TTGCACACAG TtGCACATAG TTGCACACAG TTGCACATAG TTGCACATAG TTGCGCATAG TTGCNCACAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCACACAG TTGCACACAG TTGCACACAG TTGCACACAG TTGCACACAG TTGCACACAG TTGCACATAG TTGCACACAG TTGCACACAG TTGCTCATAG TTGCACACAG tCGCCCATAG TTGCACATAG TTGCACATAG TTGCTCATGG TTGCACACAG TTGCACACAG TTGCACATAG TTGCACACAG TTGCACATAG TTGCACATAG TTGCTCATAG TTGCACATGG TTGCTCATAG TTGCACATAG TTGCACATGG TTGCTCATGG TTGCACACAG TTGCTCATAG TTGCTCATAG TTGCACATAG TTGCTCATAG TTGCACATAG TTGCTCATAG TTGCTCATGG TTGCACATGG TTGCACATAG TTGCACATAG TTGCTCATAG TTGCTCATAG ....|....| 415 AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCNTCT GGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCGTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCGTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGGTCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCTTCT AGGATCGTCT AGGATCATCT AGGGTCATCT AGGATCATCT AGGATCATCT AGGAGCTTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCTTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCA AGGTTCATCT AGGATCTTCT AGGATCTTCT AGGATCATCA AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT ....|....| 425 GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGANTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGACTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGACTTAG GTAGATTTAG GTGGATTTAG GTAGATTTAG GTAGATTTAG GTAGACTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTGGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG ....|....| 435 CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTTTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CaATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CTATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CTATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATCTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CTATTTTTTC CAATTTTCTC CCATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC ....|....| 445 TCTTCATTTA CCTTCATTTG GCTTCATTTA TCTCCATTTA TCTTCATTTG TCTTCATTTG TCTTCATTTG ACTTCATTTA ACTTCATTTA ACTTCATTTA ACTTCATTTA CCTTCATTTA TCTTCATTTA TCTTCATTTA GCTTCATTTA CCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA CCTTCATTTA CCTTCATTTA TCTCCATTTA GCTTCATTTA TTTACATTTA TCTTCATTTA CCTTCATTTA TCTTCATTTG TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA CCTTCATTTA TCTTCATTTA TCTTCATTTA ACTTCATTTA TCTTCATTTA ATTACATTTA TCTTCATTTA CCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA CCTTCACTTA TCTTCATTTA TCTTCATTTA TCTCCATTTA ACTTCATTTA CCTTCATTTA ACTTCATTTA TCTTCATTTA ATTACACTTA TTTACATTTA TCTTCATTTA TCTTCATTTA ACTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA ACTTCATTTA TCTTCATTTA CCTTCATTTA TCTTCATTTA CCTCCATTTA ACTTCATTTA ACTTCATTTA 175 ....|....| 455 GCGGGAATTT GCGGGAATTT GCCGGAATTT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCCGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCTGGAATTT GCTGGAATTT GCCGGAATTT GCTGGAATTT GCAGGAATTT GCAGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCTGGGATTT GCAGGAATTT GCAGGAATTT GCGGGAATTT GCTGGAATTT GCAGGAATTT GCTGGTATTT GCAGGAATTT GCAGGAATTT GCCGGAATTT GCTGGAATTT GCTGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCTGGAATTT GCTGGAATTT GCGGGAATTT GCTGGAATTT GCGGGAATTT GCTGGTATTT GCTGGAATTT GCTGGGATTT GCAGGAATTT GCTGGAATTT GCAGGAATTT GCAGGAaTCT GCAGGAATTT GCTGGAATTT GCTGGAATTT GCGGGAATTT GCAGGAATTT GCTGGAATTT GCTGGAATTT GCTGGAATTT ....|....| 465 CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CCTcAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTGT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTCT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CATCAATTTT CTTCAATTGT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CCTCAATTTT CTTCAATTTT CTTCAATTTT ....|....| 475 AGGGGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGaGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGGGCTATT AGGAGCAATT AGGGGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGGGCAATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGTGCTATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCTATT ....|....| 485 AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTa AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTT AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AACTTTATTA ....|....| 495 CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATtaT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTaTtAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CCACTATTAT CAACTATTAT CTACTATTAT CAACTATTAT CAACCATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CTACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATCAT CAACCATCAT CAACTATTAT CAACCATCAT CAACTATCAT CAACTATTAT CAACTATTAT CAACCATCAT CAACTATTAT CAACTATCAT CAACTATCAT ....|....| 505 TAATATACGG CAATATACGA TAATATACGA TAATATACGG CAATATACGA CAATATACGA CAATATACGA CAATATGCGA CAATATGCGA CAATATGCGA CAATATACGA TAATATGCGA TAATATACGA TAATATACGA TAATATACGA CAATATACGG TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAACATACGA TAATATACGG TAATATACGA TAATATACGA TaATATACGG TAATATACGA CAATATACGG TAACATACGG TAATATACGA TAATATACGG TAATATACGA TAATATACGG TAACATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGG TAATATACGG TAATATACGA TAATATACGA TAATATGCGG TAATAtACGA TAATATACGA TAATATACGA TAATATACGA TAATAtACGA TAATATACGA TAATATACGA CAACATACGA TAATATACGG CAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGC TAATATACGA TAATATACGA TAACATACGA TAATATACGA TAATATACGA TAATATACGA CAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA ....|....| 515 GTAAATAACT GTAAATAAAT GTAAATAATT GTAAATAATT GTAAATAACC GTAAATAACC GTAAATAACC GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAAAT GTAAATAACT GTAAATAACT GTAAATAACT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACT GTAAATAATT GTAAATAACC GTAAATAACT GTAAATAACT GTAAATAACT GTAAATAACT GTAAATAACT GTAAATAACT GTAAATAATT GTAAATAATT GTAAATAACT GTAAATAACT GTAAATAATT GTAAATAACT ATTAGTAATT GTAAATAATT GTAAATAATT GTAAATaATT GTAAATAATT GTAAATAATT GTAAATAATT GTGAATAACT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTTAATAATT GTAAATAATT ATTAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT ....|....| 525 TAACCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTtTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCCTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCATTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TAACCTTTGA TATCCTTTGA TATCCTTTGA TATCCTTTGA TATCCTTTGA TATCTTTTGA TATCCTTTGA TAACCTTTGA TATCTTTTGA TATCCTTTGA TATCCTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCATTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA ....|....| 535 TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCT TCAAATATCT TCAAATATCC TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATACCA TCAAATATCA TCAAATATCC TCAAATATCA TCAAATATCA TCAAATATCA CCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA CCAAATATCA TCAAATATCA TCAAATATCA CCAAATATCA TCAAATATCC TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA Appendix 3: Sequence data MW01105 MW01107 MW01114 MW01116 MW01120 MW02001 MW02006 MW02007 MW02008 MW02009 MW02021 MW02024 MW02025 MW02028 MW02031 MW02033 MW02034 MW98002 MW98008 MW98009 MW98020 MW98029 MW98049 MW98079 MW98084 MW98089 MW98094 MW98097 MW98103 MW98106 MW98128 MW98129 MW98133 MW98136 MW98138 MW98139 MW98154 MW98162 MW98170 MW98172 MW98180 MW98183 MW98185 MW98189 MW98203 MW98205 MW98212 MW98220 MW98228 MW98230 MW98235 MW98240 MW98261 MW98264 MW98265 MW98270 MW98274 MW98278 MW98284 MW98285 MW98294 MW98313 MW98315 MW99001 MW99006 MW99009 MW99013 MW99014 ....|....| 365 GGAACAGGAT GGAACAGGAT GGAACTGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACTGGAT GGAACTGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGGACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGGACAGGAT GGAaCAGGaT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT ....|....| 375 GAACAGTTTA GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTGTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTGTA GAACAGTTTA GAACTGTGTA GAACAGTCTA GAACAGTGTA GAACAGTGTA GAACAGTGTA GAACAGTCTA GAACAGTTTA GAACAGTGTA GAACAGttTA GAACAGTTTA GAACAGTTTA GAACGGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAAcAGTTTA GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTGTA GAACAGTTTA GAACGGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA ....|....| 385 CCCCCCACTT CCCCCCACTT CCCCCCACTA CCCCCCACTT CCCCCCACTA TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTA TCCCCCACTT CCCCCCaCTA CCCCCCACTA CCCCCCACTA CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCGCTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT Cytochrome Oxidase I (COI) ....|....| 395 TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATa TCATCtAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAACA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA ....|....| 405 TTGCACATGG TTGCACATAG TTGCACATAG TTGCTCATAG TTGCCCATGG TTGCACATAG TCGCACATAG TTGCCCATAG TTGCACATAG TTGCTCATAG TTGCTCATAG TTGCCCATAG TTGCACATAG TTGCACATGG TTGCTCATAG TTGCTCATAG TTGCACATGG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCACACAG TTGCACATAG TTGCACATGG TTGCACATAG TTGCTCATAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCACATAG TTGCTCATAG TTGCACACAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCTCATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATGG TTGCTCATAG TTGCCCATAG TCGCACATAG TTGCACATAG TTGCACATAG TCGCACATAG TTGCACATGG TTGCACATAG TTGCACATAG TTGCTCATAG TTGCACATAG TTGCACATAG TTGCACATGG TTGCACATAG TTGCACATAG TTGCTCATAG TTGCACATAG TTGCACACAG TTGCaCATAG TTGCACATAG ....|....| 415 AGGATCATCT AGGATCATCT AGGAAGATCT AGGATCATCT TGGATCATCC TGGATCATCC TGGATCTTCT AGGGTCATCT TGGATCATCT AGGATCTTCT AGGATCTTCT AGGAAGATCT AGGATCTTCA AGGTTCATCT AGGTTCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCA AGGATCATCT AGGATCTTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT aGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGTTCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCA ....|....| 425 GTAGATTTAG GTAGATTTAG GTTGATCTTG GTAGATTTAG GTTGATTTAG GTAGACTTAG GTAGATTTAG GTTGATTTAG GTAGATCTAG GTTGATTTAG GTTGATTTAG GTCGATCTTG GTCGATTTAG GTTGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGACTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTGGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGACTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG ....|....| 435 CAATTTTCTC CAATTTTCTC CTATTTTTTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CGATTTTCTC CTATTTTTTC CAATTTTCTC CTATTTTTTC CTATTTTTTC CTATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTTTC CAATTTTTTC CAATCTTTTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CtATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC TAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CTATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC TAATTTTCTC ....|....| 445 ACTTCATTTA TCTTCATTTA ACTTCACTTA ACTTCATTTA TTTACACCTA ACTTCATTTA TCTTCATTTA ATTACATTTA ATTACATTTA TTTACATTTA TTTACATTTA ACTTCACTTA TCTTCATTTA ATTACACTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TTTACACTTA TCTCCATTTA CCTTCATTTA TCTTCATTTA ACTTCATTTA TCTTCATTTA CCTTCATTTA ACTTCATTTG CCTTCATTTA TTTACATTTA CCTTCATTTA CCCCCATTTA TCTTCATTTA TCTTCATTTG ACTTCATTTA TCTTCATTTA TCTTCATTTG CCTTCATTTA TCTTCATTTG CCTTCATTTA CCTTCATTTG TCTTCATTTG TCTTCATTTA CCTTCATTTA CCTTCATTTA ACTTCATTTA CCTTCATTTG TCTTCATTTG TCTTCATTTA TCTTCATTTA tCTTCATTTA ACTTCATTTA CTTACATTTA TTTACACTTG CCTTCATTTA CCTTCATTTA TTTACACTTG ATTACACTTA TCTTCATTTA GCTTCATTTA ACTTCATTTA CCTTCATTTG CCTTCATTTA CCTTCATTTA TCTTCATTTG TCTTCATTTA TCTCCATTTA CCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTG 176 ....|....| 455 GCGGGAATTT GCAGGAATTT GCTGGTATTT GCTGGAATTT GCAGGAATTT GCTGGAATTT GCTGGAATTT GCAGGTATTT GCAGGTATTT GCAGGTATTT GCCGGAATTT GCTGGTATTT GCAGGAATTT GCTGGTATTT GCAGGAATTT GCAGGAATCT GCTGGAATTT GCAGGAATTT GCTGGAATTT GCAGGAATTT GCGGGAATTT GCTGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGTATTT GCGGGAATTT GCAGGAATTT GCGGGAATTT GCAGGAATTT GCTGGCATTT GCTGGGATTT GCAGGAATTT GCGGGAATTT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCTGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCTGGAATTT GCTGGAATTT GCAGGTATTT GCTGGAATTT GCAGGAATTT GCAGGAATTT GCTGGAATTT GCTGGTATTT GCTGGAATTT GCTGGAATTT GCTGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCTGGAATTT GCGGGAATTT GCGGGAATTT GCTGGAATTT GCAGGAATTT ....|....| 465 CTTCAATTTT CTTCAATTTT CATCTATTTT CTTCAATTTT CATCTATTTT CTTCAATTTT CTTCAATTTT CATCAATTTT CATCAATTTT CTTCAATTCT CTTCAATTTT CTTCTATTTT CATCAATTTT CTTCAATTTT CATCAATTTT CTTCAATTTT CTTCAATTCT CATCAATTTT CCTCAaTTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CATCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATCTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT ....|....| 475 AGGAGCAATT AGGAGCAATT AGGTGCAATT AGGAGCTATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGAGCTATT AGGTGCAATT AGGTGCAATT AGGAGCAATT GGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGGGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGGGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCaATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCaATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCTATT GGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGGGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCAATT ....|....| 485 AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATCA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATCA AACTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA ....|....| 495 CAACTATTAT CAACCATCAT CAACAATTAT CAACTATCAT CAACTATCAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACAATTAT CAACTATTAT CTACTATTAT CTACAATTAT CAACTATTAT CTACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTaT CAACTATTAT CAACTATtAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATCAT CaACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACAATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CTACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT ....|....| 505 CAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAACATACGA TAATATACGA CAATATACGA TAATATACGA TAACATACGT TAATATACGA TAATATACGG TAATATACGA TAATATACGA TAACATACGA TAATATACGG TAATATACGA TAATATACGG TAATATACGA TAATATACGA TAATATACGA TAATATACGA caATATACGG CAATATACGA CAATATACGG TAATATACGA CAATATACGG TAATATACGG TAATATACGA TAATATACGA TAATATACGA TAATATACGA CAATATACGA CAACATACGG CAATATACGA CAATATACGG CAATATACGG CAATATACGA TAATATACGA CAATATACGG CAATATACGG TAATATACGA CAATATACGG CAATATACGA TAACATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAACATACGA TAATATACGG TAATATACGA TAACATACGA TAATATACGA TAATATACGA TAACATGCGA TAATATACGG CAATATACGG CAATATACGG TAATATACGG CAATATACGA TAATATACGA TAACATACGG CAATATACGG TAATATACGA TAATATACGA TAACATACGA ....|....| 515 GTAAATAATT GTAAATAATT ATTAATAATA GTAAATAATT GTTAATAATC GTAAATAATT GTAAGTAATT ATTAATAATT GTAAATAATT ATTAATAATT GTAAATAATT ATTAATAATA GTAAATAATC GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTTAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTATATAAAT GTAAATAATT GTAAATAAAT ATTAATAATT GTAAATAAAT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACC GTAAATAAAT GTAAATAACC GTAAATAAAT GTAAATAAAT GTAAATAACC GTAAATAATT GTAAATAAAT GTAAATAAAT GTAAATAATT GTAAATAAAT GTAAATAACC GTAAATAATT GTAAATAATT GTAAATAGTT GTAAATAATT ATTAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAAAT GTAAATAAAT GTAAATAATT GTAAATAACC GTAAATAATT GTAAATAACT GTAAATAAAT GTAAATAATT GTAAATAAAC GTAAATAATT ....|....| 525 TATCTTTTGA TATCTTTTGA TATCATTTGA TATCTTTTGA TTTCATTCGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCATTTGA TATCATTTGA TATCATTTGA TATCATTTGA TATCATTTGA TATCTTTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCCTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCATTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCATTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCCTTTGA ....|....| 535 TCAAATATCC TCAAATATCA TCAAATACCT TCAAATATCA TCAAATATCT TCAAATATCC TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA CCAAATATCA TCAAATaCCT TCAAATATCA TCAAATATCA TCAAATATCA CCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCC TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCG TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA CCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCG TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA CCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA Appendix 3: Sequence data MW99018 MW99028 MW99038 MW99042 MW99045 MW99047 MW99053 MW99057 MW99058 MW99059 MW99060 MW99061 MW99068 MW99080 MW99084 MW99094 MW99095 MW99097 MW99102 MW99104 MW99105 MW99124 MW99128 MW99134 MW99135 MW99140 MW99141 MW99158 MW99163 MW99164 MW99165 MW99170 MW99174 MW99187 MW99196 MW99203 MW99221 MW99226 MW99240 MW99247 MW99263 MW99264 MW99274 MW99276 MW99286 MW99289 MW99292 MW99301 MW99303 MW99309 MW99314 MW99319 MW99320 MW99341 MW99344 MW99353 MW99357 MW99372 MW99374 MW99376 MW99380 MW99381 MW99382 MW99393 MW99398 MW99406 MW99407 MW99408 ....|....| 365 GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGaACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAaCAGGAT GGAACAGGAT GGAACAGGGT GGAACAGGGT GGAACAGGGT GGAACAGGAT GGGACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGGT GGGACTGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACTGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT ....|....| 375 GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTTTA GAACAGTGTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTGTA GAACAGTGTA GAACAGTTTA GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACGGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTGTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GANCaGTTTa GAACAGTTTA ....|....| 385 CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACCA CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCTCCACTT CCCTCCACTT CCCACCACTT CCCCCCACTT CCCCCCGCTT CCCCCCACTT CCCCCCACTT CCCTCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTC CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCaCTT CCCCCCACTT Cytochrome Oxidase I (COI) ....|....| 395 TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCtAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCCAATA TCATCAAATA TCATCAAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAAtA TCATCTAATA TCATCTAATA TCATCTAATA ....|....| 405 TTGCTCATGG TtGCTCATGG TCGCACATAG TTGCtCATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATGG TTGCCCATAG TTGCaCATAG TTGCACATAG TTGCACATAG TTGCTCATAG TTGCACATGG TTGCACATGG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCTCATAG TTGCACATGG TTGCCCATAG TTGCACATAG TCGCACATAG TTGCACATAG TTGCACACAG TTGCACACAG TTGCACACAG TTGCACATGG TTGCACACAG TTGCTCATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATGG TTGCACATGG TCGCACATAG TCGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATGG TTGCACACAG TTGCACATAG TCGCACATAG TCGCACATAG TTGCACATAG TTGCACACAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCACATAG TTGCACATAG TCGCACATAG TTGCACATGG TTGCACATGG TTGCACATAG TTGCTCATAG TTGCACATAG TTGCACATGG TTGCACATAG ....|....| 415 AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT TGGAtCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT GGGATCATCT GGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT GGGATCATCT AGGATCATCA AGGATCCTCA AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGTTCTTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCCTCA AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCGTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCA AGGATCATCT AGGNTCNTCT AGGATCATCT ....|....| 425 GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATCTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTAGATCTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGACTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATCTAG GTAGATTTAG GTAGANTTAG GTAGATTTAG ....|....| 435 CAATTTTTTC CAATTTTTTC CAATTTTCTC CAATTTTCtC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CTATTTTTTC CAATTTTTTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CTATTTTTTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CTATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC ....|....| 445 TCTTCATCTA TCTTCATTTA TTTACACTTG ACTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTG CCTTCATTTG TCTTCATTTG CCTTCACTTA CCTTCATTTA ACTTCATTTA ACTTCATTTA ATTACATTTA TCTTCATTTG TCTTCATTTA TCTCCATTTA ACTTCATTTA ACTTCATTTA ACTTCATTTA ACTTCATTTA TCTCCATTTA ACTTCATTTA TCTTCATTTA ACTTCATTTA ACTTCATTTA TTTACATTTA TCTTCATCTA TCTTCATTTA TCTTCATTTG TCTTCATTTA CCTTCATTTA CCTTCATTTA ACTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTG CCTTCATTTG ACTTCATTTA ACTTCATTTA TCTTCATTTA TCTTCATTTA CCTTCATTTA CCTTCACTTA CCTTCATTTA TCTTCATTTA TCTTCATCTA TCTCCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA CCTTCATTTA ACTCCATTTA TCTTCATTTG TCTTCATTTG ACTCCATTTA TCTTCATTTA CCTTCATTTA TCTTCATTTA ACTTCATTTA ACTTCATTTA CCTTCATTTA TCTTCATTTA CCTTCATTTA ACTTCANTTA CCTTCATTTA 177 ....|....| 455 GCTGGAATTT GCAGGAATCT GCTGGAATTT GCTGGCATTT GCAGGAATTT GCTGGAATTT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGTATTT GCGGGAATTT GCAGGAATTT GCTGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCTGGAATTT GCTGGAATTT GCAGGAATTT GCTGGGATTT GCGGGAATTT GCAGGTATTT GCTGGAATTT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCGGGAATTT GCAGGAATTT GCTGGAATCT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGGATTT GCAGGAATTT GCAGGAATTT GCTGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGtATCT GCAGGAATTT GCGGGAATTT GCGGGAATTT ....|....| 465 CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTcAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CATCAATTTT CATCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CCTCAATTTT CTTCTATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CATCAATCTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATCTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CATCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT ....|....| 475 AGGAGCTATT AGGAGCTATT AGGAGCTATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGNGCAATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT aGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGGGCAATT NNNNNNNNNN AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCaATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGaGCCATT AGGAGCAATT AGGAGCAATT AGGAGCAATT ....|....| 485 AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTC AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA NNNNNNNNNN AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA ....|....| 495 CAACTATTAT CAACTATTAT CAACTATTAT CAACTAGCAT CAACAATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTaT CTACAATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CTACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATcAT CAACTATTAT CAACTATTAT CAACTATTAT NNNNNNNNNN CAACTATTAT CAACTATTAT CAACAATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATtAT CAACTATTAT ....|....| 505 TAATATACGa TAATATACGA TAACATACGA TAATATACGA TAATATAAAA TAATATACGG TAATATACGG CAATATACGA CAATATACGG CAATATACGA TAATATACGA CAATATACGG CaATATACGA TAATATACGA CAATATACGA CaATATACGA TAATATACGA TAACATACGA CAATATACGA CAATATACGA CAATATACGA TAATATACGA TAATATACGG TAACATACGA TAATAGACGG TAATATACGA CAATATACGA TAATATACGA TAATATACGA TAATATACGA CAATATACGA TAATATACGA TAATATACGG NNNNNNNNNN CAATATACGA TAATATACGG TAATATACGA TAATATACGG CAATATACGA CAATATACGA CAATATACGA CAATATACGA TAATATACGA TAATATACGA TAATATGCGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGG TAATATACGG TAATATACGA TAATATACGA TAATATACGA TAATATACGG CAATATACGA CAATATACGA TAATATACGG TAATATACGG TAATATGCGA TAATATACGA CAATATGCGA CAATATGCGA TAATATGCGA NNNNNNNNNN TAATATGCGA CAATATACGA CAATATACGG ....|....| 515 GTAAATAATT GTAAATAATT GTAAATAATT GTaAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACC GTGAATAAAT GTAAATAACC GTAAATAATT GTAAATAAAT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACC GTAAATAATT GTAAATAACT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTNAATAATT GTAAATAATT GTAAATAATT GTAAATAATT aTTAATAATT GTAAATAATT GTAAATAATT GTAAATAACC GTAAATAATT GTAAATAACT NNNNNNNNNN GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACC GTAAATAAAT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAGTT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACC GTAAATAACC GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT NNNNNNNNNN GTAAATAATT GTAAATAAtT GTAAATAAAT ....|....| 525 TATCTTTTGa TATCATTTGA TATCATTTGA TATCTTTTGA TATTTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TaTCTTTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TAACCTTTGA NNNNNNNNNN TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA NNNNNNNNNN TATCTTTTGA TATCTTTTGA TATCCTTTGA ....|....| 535 TCAAATaTCa CCAAATATCA TCAAATATCA TCAAATATCG TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCC TCAAATATCA TCAAATATCC TCAAATATCA TCAAATATCA CCAAATATCA TCAAATATCA TCAAATATCC TCAAATATCC TCAAATATCA TCAAATATCA TCAATTATCA TCAAATATCA TCAAATATCA TCAAATATCC TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA NNNNNNNNNN TCAAATATCC TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCC TCAAATATCC TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCT TCAAATATCA NNNNNNNNNN TCAAATATCA TCAAATATCC TCAAATATCA Appendix 3: Sequence data Cytochrome Oxidase I (COI) MW99413 MW99422 MW99425 MW99430 MW99435 MW99448 MW99449 MW99465 MW99469 MW99471 MW99473 MW99476 MW99479 MW99494 MW99501 MW99508 MW99514 MW99515 MW99520 MW99526 MW99536 MW99537 MW99538 MW99546 MW99550 MW99552 MW99559 MW99565 MW99579 MW99591 MW99605 MW99608 MW99612 MW99613 OK96022 OK99001 WE00002 WE00003 WE02321 WE02421 WE02431 WE02451 WE02491 WE02531 WE02535 WE02536 WE02591 WE02612 WE02614 WE02621 WE02661 WE02662 WE02671 WE02674 WE02676 WE02677 WE85001 WE98001 ....|....| 365 GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGGT GGAACGGGAT GGAACGGGAT GGAACAGGAT GGAACAGGGT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACAGGAT GGAACGGGAT GGAACAGGAT ....|....| 375 GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAGNAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTCTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA GAACAGTTTA ....|....| 385 CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT TCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT CCCCCCACTT ....|....| 395 TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAACA TCATCTAACA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCAAATA TCATCAAATA TCATCTAATA TCATCAAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAACA TCATCTAATA TCATCCAATA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAACA TCATCTAATA TCATCTAATA TCATCTAATA TCATCTAATA ....|....| 405 TTGCACATAG TTGCACATAG TTGCTCATGG TTGCACACAG TTGCTCATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TCGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCTCATAG TGGCTCATAG TTGCCCATAG TTGCACACAG TTGCTCATAG TCGCACATAG TCGCACATAG TTGCACATAG TCGCACATAG TTGCACATAG TTGCACACAG TTGCACACAG TTGCACACAG TTGCACATGG TTGCACATGG TTGCTCATAG TTGCCCATAG TTGCACATAG TTGCTCATAG TTGCTCATAG TTGCGCACAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATGG TTGCACATAG TTGCACACAG TTGCACATAG TTGCACATGG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACACAG TTGCACATAG TTGCACATAG TTGCACATAG TTGCACATGG TTGCACACAG TTGCACATAG ....|....| 415 AGGATCATCT AGGATCATCT AGGATCTTCA AGGGTCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGACCTTCT AGGATCTTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCTTCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT AGGATCATCT ....|....| 425 GTAGATTTAG GTAGATTTAG GTTGATTTAG GTTGATTTAG GTAGATTTAG GTaGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTTGATTTAG GTTGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAA GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAA GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG GTAGATTTAG ....|....| 435 CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTTTC CAATTTTCTC CAATTTTTTC CAATTTTTTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CTATTTTTTC CTATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTNTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTTTC CAATTTTCTC CAATTTTCTC CAATTTTCTC CAATTTTCTC ....|....| 445 CCTTCATTTG CCTTCATTTG ATTACACTTA TCTTCATTTA ACTTCATTTA TCTTCATTTG TCTTCATTTG TCTTCATTTG TCTTCATTTA TCTTCATTTA CCTTCATTTA TCTTCATTTA GCTTCATTTA TCTTCATCtA CCTTCATTTA TCTTCATTTA ACTTCATTTA TTTACATTTA TTTACATTTA TCTTCATTTA ACTTCATTTA TTTACACCTG TTTACACTTG ACTTCATTTG TTTACACCTG TCTTCATTTA TCTCCATTTA TCTCCATTTA TCTTCATTTA TCTTCATTTA TCTTCATTTA ACTTCATTTA ACTTCAATTA ACTTCATTTA ACTTCATTTA ACTTCATTTA TCTTCATTTA TCTTCACTTA TCTTCATTTA TCTTCATTTG ACTTCATTTA TCTTCATTTA TCTTCATTTA GCTTCATTTA ACTTCATTTA TCTTCATTTG CCTTCATTTA CCTTCATTTA CCTTCATTTA TCTTCATTTG GCTTCATTTA TCTCCATTTA CCTTCATTTA TCTTCATTTG TCTTCATTTA ACTTCATTTA TCTTCATTTA TCTTCATTTA ....|....| 455 GCGGGAATTT GCGGGAATTT GCTGGTATTT GCTGGAATTT GCTGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCTGGGATTT GCAGGTATTT GCAGGTATTT GCAGGAATTT GCTGGCATTT GCTGGAATTT GCTGGAATTT GCAGGAATTT GCTGGAATTT GCAGGAATTT GCAGGAATTT GCAGGAATTT GCTGGAATTT GCGGGAATTT GCGGGAATTT GCTGGAATTT GCTGGAATTT GCGGGAATTT GCTGGAATTT GCTGGAATTT GCAGGAATTT GCTGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCAGGAATTT GCAGGAATTT GCCGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCGGGAATTT GCCGGAATTT GCAGGAATTT GCAGGAATTT GCGGGAATTT GCAGGAATTT GCGGGAATTT GCAGGAATTT GCGGGAATTT ....|....| 465 CTTCAATTTT CTTCAATTTT CCTCAATCTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTtT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT CTTCAATTTT ....|....| 475 AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGTGCTATT AGGAGCCATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGAGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCTATT AGGAGCTATT AGGGGCAATT AGGAGCTATT AGGAGCTATT AGGAGCAATT AGGGGCTATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGGGCAATT AGGAGCAATT AGGGGCAATT AGGGGCAATT AGGGGCAATT AGGAGCAATT AGGGGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT AGGAGCAATT ....|....| 485 AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTaTTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AACTTTATTA AACTTTATTA AATTTTATTA AACTTTATTA AACTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATtA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTA AATTTTATTC AATTTTATTA ....|....| 495 CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATCAT CAACTATCAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATCAT CAACTATCAT CAACTATTAT CAACTATCAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT CTACTATTAT CAACTATTAT CAACTATTAT CAACTATTAT ....|....| 505 CAATATACGA CAATATACGA TAATATACGA TAATATGCGA TAATATACGG CAATATACGA CAATATACGA CAATATACGA TAATATACGA TAATATACGA TAATATGCGA TAATATACGG TAATATACGG TAATATACGA CAATATACGG TAATATACGG TAATATACGA CAATATACGA TAATATACGA TAATATACGG TAATATACGA TAACATACGA TAACATACGA TAACATACGA TAACATACGA TAATATACGG TAATATACGG TAATATACGG TAATATACGA TAATATACGA TAATATACGA TAATATACGG TAATATACGA TAACATACGA TAATATACGA TAATATACGA TAATATACGA TAATATACGA CAATATACGA CAATATACGA CAATATACGA TAATATACGG TAATATACGG TAATATACGA CAATATACGA CAATATACGA TAATATACGA TAATATACGA TAATATACGA CAATATACGA TAATATACGA TAATATACGG TAATATGCGA CAATATACGA TAATATACGG CAATATGCGA TAATATACGG TAACATACGA AD98001 AD98009 AD98012 AD98018 AD98024 AD98029 AD98036 ....|....| 545 TTATTTATTT CTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT ....|....| 555 GAGCAGTAGG GAGCTGTGGG GAGCTGTAGG GAGCAGTGGG GAGCAGTGGG GAGCAGTGGG GAGCAGTAGG ....|....| 565 AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA ....|....| 575 TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC ....|....| 585 TTTTATCTTT TTCTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT ....|....| 595 ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ....|....| 605 GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA ....|....| 615 TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT ....|....| 625 ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACTGAT ....|....| 635 CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTa ....|....| 645 ACACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACCTCATT ATACCTCATT aTACCTCTTT ....|....| 655 TTTTGATCCA CTTTGACCCA CTTTGACCCA CTTTGATCCA CTTTGATCCA CTTTGATCCA CTTTGACCCN ....|....| 665 GCTGGTGGAG GCTGGGGGAG GCTGGGGGAG GCTGGTGGAG GCTGGTGGAG GCTGGTGGaG GCTGGTGGGG ....|....| 675 GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCNNNN GNNNNNNNNN GNNNNNNNNN ....|....| 685 TTTATACCAA TTTATACCAA TTTATACCAA TTTANNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 178 ....|....| 515 GTAAATAAAT GTAAATAAAT GTAAATAATT GTAAACAATA GTAAATAATT GTAAATAACC GTAAATAACC GTAAATAACC GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAAAT GTAAATAATT GTAAATAATT ATCAATAATT ATTAATAACT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAATT GTAAGTAATT GTAAATAACC GTAAATAACC GTAAATAATT GTAAATAAAT GTAAATAACT GTAAATAATT GTAAATAATT GTAAATAACC GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACC GTAAATAATT GTAAATAATT GTAAATAATT GTAAATAACC GTAAATAATT GTAAATAATT GTAAATAACT GTAAATAATT ....|....| 525 TATCTTTTGA TATCTTTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCATTTGA TATCATTTGA TATCTTTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCATTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCCTTTGA TATCTTTTGA TGTCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCTTTTGA TATCCTTTGA TATCATTTGA ....|....| 535 TCAAATATCA TCAAATATCA TCAAATATCC TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCC TCAAATATCA TCAAATATCG TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA ACAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA CCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCC TCAAATATCA tCAAATATCA TCAAATATCA TCAAATATCC TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCA TCAAATATCT TCAAATATCA TCAAATATCA Appendix 3: Sequence data DS00001 DS00005 DS01001 JC00029 JC00039 JC00040 JC00042 JC00043 JC00045 JC00046 JC00051 JC00055 JC00057 JC00061 JC00062 JC00063 JC01014 JC02001 JM00001 MW00001 MW00015 MW00017 MW00020 MW00024 MW00032 MW00044 MW00051 MW00056 MW00058 MW00059 MW00060 MW00064 MW00072 MW00076 MW00087 MW00101 MW00102 MW00110 MW00116 MW00119 MW00125 MW00127 MW00129 MW00140 MW00151 MW00157 MW00176 MW00177 MW00178 MW00179 MW00183 MW00185 MW00186 MW00189 MW00226 MW00229 MW00231 MW00232 MW00234 MW00259 MW00262 MW00267 MW00269 MW00290 MW00302 MW00316 MW00326 MW00327 ....|....| 545 TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATtT TTATTTATTT TTATTTGTTT TTATTTGTTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT CTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTGTTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT ....|....| 555 GAGCAGTAGG GAGCAGTAGG GAGCAGTGGG GAGCAGTAGG GAGCAGTAGG GAGCAGTGGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCTGTTGG GAGCAGTGGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GGGCGGTAGG GAGCAGTAGG GAGCTGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCGGTAGG GGGCAGTAGG GAGCGGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCGGTAGG GAGCAGTAGG GAGCGGTCGG GAGCTGTAGG GAGCTGTGGG GAGCAgTAGG GGGCTGTAGG GAGCGGTAGG GAGCTGTGGG GAGCAGTAGG GAGCAGTGGG GGGCTGTAGG GGGCTGTAGG GGGCTGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTGGG GAGCAGTGGG GAGCAGTAGG GAGCTGTGGG GAGCAGTAGG GAGCAGTAGG GAGCGGTAGG GAGCGGTAGG GAGCAGTAGG GAGCGGTAGG GAGCAGTAGG GAGCGGTGGG GAGCAGTaGG ....|....| 565 AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA TATTACCGCA TATTACTGCA AATTACAGCA TATTACTGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACGGCA AATTACAGCA AATTACAGCT TATTACTGCA TATTACTGCT AATTACGGCA AATTACAGCT AATTACGGCA AATTACGGCA AATTACGGCA AATTACGGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACTGCA AATTACAGCT AATTACAGCA AATTAcGGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACGGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACGGCA AATTACAGCA AATTACAGCA AATTACGGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACGGCA AATTACAGCA AATTACaGCA Cytochrome Oxidase I (COI) ....|....| 575 TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAT TTATNNNNNN CTATTATTAC TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTACTATTAT TTATTATTAC TTAtTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TtATTATtAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC CTATTATTAC CTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATtAC TTATTATTAC TTATTGTTAC ....|....| 585 TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT TTTTATCCTT TTTTATCTTT TATTATCTTT NNNNNNNNNN TTTTATCTTT TTTTATCCTT TATTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCCCT TATTATCTTT TTCTATCTTT TTTTATCTTT TTCTATCTTT TTTTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTCTATCTTT TTCTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT TTTTATCATT TTTTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TCTTATCTTT TTCTGTCTTT ....|....| 595 ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA GCCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCAGTATTA ACCTGTATTA ACCTGTATTA ACCCGTATTA ACCTGTTTTA NNNNNNNNNN ACCTGTATTA ACCTGTATTA ACCTGTTTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCAGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTTTTA ACCTGTATTA AcCTGTaTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCCGTATTA ACCCGTATTA ACCCGTATTA ACCTGTATTA ACCTGTaTTA GCCTGTATTA GCCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCtGTATTA ....|....| 605 GCTGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCaA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCaA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCaA GCTGGAGCaA GCTGGAGCAA GCTGGAGCTA NNNNNNNNNN GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGGGcAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGaGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCAGGAGCTA GCAGGAGCTA GCTGGAGCAA GCTGGAGCAA GCAGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA ....|....| 615 TTACCATATT TTACCATGTT TTACCATATT TTACTATATT TCACTATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACTATaTT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT NNNNNNNNNN TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACTATATT TTACCATATT TTaCCATATT TTACCATATT TTACTATGTT TTACTATATT TTACCATATT TTACCATATT TNACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATACT TTACTATATT TTACCATATT TTACTATATT TTACTATATT ....|....| 625 ATTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACGGAT ATTAACTGAT ACTTACCGAT ATTAACAGAT ATTAACCGAT ATTAACCGAT ATTAACTGAT NNNNNNNNNN ATTAACTGAT ATTAACCGAT ATTAACTGAT ATTAACCGAT ATTAACCGAT ATTAACCGAT ATTAACCGAT ATTAACTGAT ATTAACAGAT ATTAACTGAT ATTAACCGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACCGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACCGAT ATTAACNGAT ATTAACCGAT ATTAACTGAT ATTAACAGAT ATTAACAGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACTGAT ATTaACTGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT 179 ....|....| 635 CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAACCTCA CGAAATCTTA CGAAATCTTA CGAAACCTCA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAACCTCA CGAAATATTA CGAAATATTA CGAAACCTCA CGAAATATTA CGAAACCTCA CGAAATCTTA CGAAATTTAA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGTAATCTAA NNNNNNNNNN CGAAATCTTA CGAAACCTTA CGTAATTTAA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTAA CGAAACCTAA CGGAACCTTA CGAAACCTTA CGAAATCTTA CGGAATCTTA CGTAATTTAA CGAAACCTTA CGAAATCTTA cGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAACCTAA CGAAATCTAA CGAAATCTTA CGAAATTTAA CGAAACCTTA ....|....| 645 ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ATACCTCATT ACACCTCATT aTACCTCATT ATACCTCATT ATNNNNNNNN ATACCTCATT ACACCTCATT ATACTTCATT aTACCTCATT ACACCTCATT ACACCTCATT ATACATCTTT NNNNNNNNNN ACACTTCATT ACACCTCATT ATACTTCATT ACACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACCTCATT ATANNNNNNN ATACTTCTTT ATACCTCATT ACACCTCATT aNACNTCATT ATACCTCATT ATACCTCATT ACACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ACACCTCGTT ACACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ATACCTCATT ATACCTCGTT ATACCTCGTT ATACCTCATT ATACCTCATT ATACCNNNNN ACACCTCATT ACACCTCATT ACACCTCATT ATACCTCATT ....|....| 655 CTTTGACCCA CTTTGATCCA CTTTGATCCA CTTTGATCCA CTTTGATCCT CTTTGATCCA CTTTGATCCA CTTTGACCCG CTTTGACCCG CTTTGACCCG CTTTGATCCA TTTTGATCCT TTTTGATCCT CTTTGATCCA NNNNNNNNNN CTTTGATCCA CTTTGACCCG TTTTGATCCA TTTTGACCCN CTTTGATCCG CTTTGACCCA TTTTNNNNNN NNNNNNNNNN TTTTGATCCT CTTTGATCCG TTTTNNNNNN CTTTGATCCA CTTTGATCCA CTTTGATCCA CTTTGATCCA CTTTGATCCA CTTTGACCCG CTTTGATCCA CTTTGATCCT CTTTGATCCT CTTTGACCCG CTTTGATCCA CTTTGATCCA NNNNNNNNNN TTTTGATCCT CTTTGACCCA NTTTGATCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGATCCA CTTTGACCCG CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGATCCG CTTTGATCCG CTTTGACCCA CTTTGACCCA CTTTGATCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGATCCA CTTTGATCCG NNNNNNNNNN CTTTGATCCT CTTTGATCCA TTTTGACCCT CTTTGATCCC ....|....| 665 GCTGGTGGAG GCTGGTGGAG GCTGGTGGGG GCTGGAGGGG GCAGGAGGAG GCTGGTGGGG GCTGGAGGGG GCTGGTGGAG GCTGGTGGAG GCTGGTGGAG GCTGGAGGGG GCTGGAGGAG GCTGGaGGAG GCTGGAGGAG NNNNNNNNNN GCTGGAGGGG GCTGGTGGAG GCAGGAGGAG GCTGGCGGAG GCTGGTGGGG GCTGGTGGGG NNNNNNNNNN NNNNNNNNNN GCCGGAGGAG GCTGGTGGGG NNNNNNNNNN GCTGGTGGAG GCTGGTGGAG GCTGGTGGAG GCTGGTGGAG GCTGGTGGGG GCTGGCGGAG GCTGGTGGGG GCTGGTGGAG GCTGGTGGAG GCTGGTGGAG GCTGGTGGGG GCTGGTGGAG NNNNNNNNNN GCAGGAGGTG GCTGGGGGAG GCTGGTGGAG GCTGGAGGAG GCTGGTGGGG GCTGGGGGAG GCTGGTGGAG GCTGGTGGAG GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG GCTGGTGGGG GCTGGTGGGG GCTGGTGGGG GCTGGTGGAG GCTGGTGGGG GCAGGTGGGG GCAGGTGGGG GCTGGTGGAG GCTGGGGGAG NNNNNNNNNN GCTGGCGGGG GCTGGTGGGG GCTGGTGGAG NNNNNNNNNN GCTGGAGGAG GCTGGTGGAG GCTGGAGGAG GCTGGTGGAG ....|....| 675 GAGATCCAAT GCGATCCAAT GAGATCCATT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCNNN GAGATCCTAT GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCTAT GAGATCCAAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN NNNNNNNNNN GAGATCCAAT GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGAtCCTAT GAGATCCAAT GAGATCCTAT GAGATCCCAT GAGATCCAAT GAGATCCTAT GAGATCCTAT GAGATCCAAT NNNNNNNNNN GAGACCCAAT GAGATCCTAT GNNNNNNNNN GAGATCCAAT GAGATCCTAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN GGGATCCTAT GAGANNNNNN GAGATCCAAT NNNNNNNNNN GNNNNNNNNN GAGATCCAAT GAGATCCTAT GAGATCCTAT ....|....| 685 TTTATATCAA TTTNNNNNNN TTNATATCAA TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAA TTTATATcNN TTTNNNNNNN TTTATTTCNN TTTATATCAA NNNNNNNNNN TTTNNNNNNN TTTATATCAA NNNNNNNNNN TTTATATCAA TTTATATCAA TTTATATCAA TTTANNNNNN TTTATACCAN TTTATATCAA NNNNNNNNNN NNNNNNNNNN TTTATATCAA TTTATACCAA NNNNNNNNNN TTTATACCAN TTTATACCNN TTTATACCAA TTTATACCAA TTTATATCAA TTTATNNNNN TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAA TTTATACCAA NNNNNNNNNN TTTATATCAA TTTANNNNNN NNNNNNNNNN TTTATNNNNN TTTATATCAA TTTATACCAA TTTATACCAA TTTATATCAA TTTATACCAA TTTATACCAA TTTATACCAA TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAA TTTATANCAA TTTATATCAA TTTATATCAA TTTATACCAA TTTATACCNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TTTATATCAA NNNNNNNNNN NNNNNNNNNN TTTATACCAA TTTATATCAA TTTATATCAA Appendix 3: Sequence data MW00328 MW00330 MW00335 MW00347 MW00348 MW00358 MW00393 MW00409 MW00412 MW00424 MW00444 MW00452 MW00469 MW00476 MW00497 MW00498 MW00504 MW00517 MW00525 MW00530 MW00539 MW00547 MW01001 MW01009 MW01011 MW01014 MW01018 MW01019 MW01022 MW01023 MW01025 MW01027 MW01034 MW01039 MW01048 MW01053 MW01059 MW01061 MW01072 MW01078 MW01083 MW01092 MW01103 MW01105 MW01107 MW01114 MW01116 MW01120 MW02001 MW02006 MW02007 MW02008 MW02009 MW02021 MW02024 MW02025 MW02028 MW02031 MW02033 MW02034 MW98002 MW98008 MW98009 MW98020 MW98029 MW98049 MW98079 MW98084 ....|....| 545 TTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTGTAT TTATTCATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATCT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT TTATTTATCT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTGTTT TTATTTATTT CTATTTATTT TTATTTATTT ....|....| 555 GAGCGGTAGG GGGCTGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCGGTAGG GAGCGGTAGG GAGCGGTAGG GAGCAGTAGG GAGCGGTAGG GAGCTGTAGG GGGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCGGTAGG GAGCGGTAGG GAGCAGTGGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCGGTAGG GAGCTGTTGG GAGCCGTTGG GAGCAGTAGG GAGCAGTAGG GAGCGGTAGG GAGCAGTGGG GAGCAGTAGG GAGCAGTGGG GAGCGGTAGG GAGCTGTAGG GAGCAGTAGG GAGCAGTGGG GAGCAGTAGG GAGCGGTAGG GAGCGGTAGG GAGCAGTAGG GAGCAGTGGG GAGCTGTTGG GAGCGGTAGG GAGCTGTTGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCTGTAGG GAGCTGTTGG GAGCTGTTGG GAGCTGTAGG GAGCTGTTGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCTGTAGG GAGCGGTAGG GAGCAGTAGG GAGCAGTAGG GAGCGGTAGG GAGCAGTGGG GAGCTGTAGG GAGCAGTAGG ....|....| 565 AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAgCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCG AATTACAGCA AATTACAGCA TATTACAGCT AATCACCGCT AATTACAGCA AATTACAGCA AATTACNNNN AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA TATTACAGCA AATTaCAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA TATTACCGCA AATTACAGCA AATTACAGCA TATTACTGCA AATTACAGCA AATTACAGCT AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA TATCACAGCA AATTACAGCA AATTACAGCA AATTACAGCT AATTACAGCA AATTACAGCT TATTACAGCT AATTACAGCA AATTACAGCA TATTACAGCA TATTACAGCA TATTACCGCA AATCACCGCT AATTaCAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACTGCA AATTACAGCA Cytochrome Oxidase I (COI) ....|....| 575 TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTGTTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAC TTACTATTAC CTATTATTAC TTATTATTAC TTATTATTAC NNNNNNNNNN TTATTATTAC TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAT TTATTACTAT TTATTATTAC TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAT TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTGTTATTAT TTATTACTAC TTATTATTAC TTATTATTAC CTATTATTAC CTATTACTTC TTATTATTAT TTATTACTTC TTATTATTAT TTATTATTAC TTATTATTAT TTATTATTAT TTATTATTAC CTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC ....|....| 585 TTTTATCTTT TTCtATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTCTGTCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TATTATCTTT TTTTATCTTT TATTATCATT TTTTATCATT TTTTATCTTT TTTTATCATT TTTTATCTTT TTCTATCTTT TTTTATCTTT NNNNNNNNNN TTTTATCATT TTTTATCTTT TATTATCTTT TTTTATCTTT TATTATCTTT TTTTATCTTT TATTATCTTT TATTATCTTT TTTTATCTTT TTTTATCTTT TATTATCTTT TTTTATCATT TTTTATCTTT TTTTATCATT TATTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT TTTTATCTTT TATTATCTTT TATTATCTTT TTTTATCTTT TTTTATCATT TTTTATCTTT TATTATCTTT TTTTATCTCT TTTTATCTTT TTCTATCTTT TACTATCTTT TTCTTTCTTT TATTATCTTT TTTTATCTTT TATTATCCTT TTTTATCTTT TATTATCTTT TATTATCTTT TTTTATCTTT TTTTATCTTT TGTTATCATT TTTTATCTTT TTTTATCTTT TTTTATCATT TTTTATCTTT TTTTATCTTT TTCTATCTTT TTTTATCTTT ....|....| 595 ACCTGTATTA ACCTGTATTA ACCTGTGTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTGTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCCGTATTA ACCTGTATTA ACCTGTATTA ACCAGTATTA ACCTGTATTA ACCTGTATTA ACCTGTTTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA NNNNNNNNNN ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTTTTA ACCTGTATTA ACCTGTATTA ACCAGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCAGTATTA ACCTGTATTA TCCAGTTTTA ACCTGTATTA ACCGGTATTG ACCTGTTTTA ACCTGTTTTA ACCTGTTTTA ACCTGTTTTA ACCAGTATTA ACCAGTATTA ACCTGTTTTA ACCTGTTTTA ACCTGTATTA ACCTGTATTA aCCAGTTTTA ACCTGTAtTA ACCTGTATTA GCCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ....|....| 605 GCTGGAGCAA GCTGGaGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCTA GCTGGGGCAA GCTGGAGCAA GCTGGAGCAA GCTGGGGCAA GCTGGAGCAA GCtGGaGCaA NNNNNNNNNN GCTGGAGCAA GCTGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCGGGAGCTA GCAGGAGCTA GCAGGAGCAA GCAGGTGCTA GCTGGAGCTA GCTGGAGCAA GCTGGGGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCTA GCTGGGGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGTGCTA GCTGGAGCTA GCAGGGGCTA GCTGGAGCTA GCTGGTGCTA GCGGGAGCTA GCGGGTGCTA GCTGGAGCAA GCTGGGGCAA GCTGGAGCTA GCTGGNNNNN GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA ....|....| 615 TTACCATATT TTACCATATT TCACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TCACCATATT TTaCTATATT TTACCATATT TTACCATATT TTaCCATATT TTACTATATT TTACCATATT TTACTATATT TTACCATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACCATATT NNNNNNNNNN TTACCATATT TTACTATATT TTACTATATT TTACCATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACCATATT TTACTATATT TTACCATATT TTACTATATT TTACTATATT TTACCATATT TTACCATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACCATATT TTACCATATT TTACTATATT TTACTATACT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACAATATT TTACTATATT TTACCATATT tTACCATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACAATATT NNNNNNNNNN TTACTATATT TTACTATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT ....|....| 625 ATTAACCGAT ATTAACTGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACGGAT ATTAACCGAT ATTAACTGAT ATTAACTGAt ATTAACTGAT ATTAACTGAT ATTAACAGAC ATTAACAGAT ATTAACTGAT ATTAACTGAC ATTAACTGAT ATTAACTGAT ATTAACCGAT NNNNNNNNNN ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACTGAT ACTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACTGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACAGAT ACTTACTGAT ATTAACTGAT ACTAACCGAT ACTAACTGAT ACTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAC ATTAACAGAT ACTTACGGAT ATTAACAGAT ATTAACTGAT ACTAACAGAT ATTAACTGAT ATTAACCGAT ATTAACTGAT NNNNNNNNNN ATTAACCGAT ATTAACAGAT ATTAACCGAT ATTAACCGAT ATTAACTGAT ATTAACCGAT 180 ....|....| 635 CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAaCCTTA CGAAACCTTA CGAAATCTTA CGAAACCTCA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAATCTCA CGAAACCTTA CGAAATTTAA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA CGAAACCTCA CGAAATCTTA NNNNNNNNNN CGAAATCTTA CGAAACCTTA CGAAATCTCA CGAAATCTTA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAATTTAA CGAAACCTCA CGAAATTTAA CGAAATCTTA CGAAATCTTA CGAAATATTA CGAAATCTTA CGAAATCTTA CGAAATATTA CGAAATCTTA CGAAATCTTA CGAAATCTAA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATTTAA CGAAATCTTA CGAAATTTAA CGAAACCTTA CGAAACCTTA CGTAATCTAA CGAAATCTTA CGTAATTTAA CGAAATTTAA CGAAATTTAA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATATTA CGAAATCTTA CGAAATTTAA NNNNNNNNNN CGAAATCTTA CGAAATCTTA CGAAACCTAA CGAAACCTTA CGAAACCTTA CGAAATCTTA ....|....| 645 ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACTTCATT ATACCTCATT ATACtTCATT ATACCTCATT ATACCTCATT ATACTTCATT ATACTTCATT ATACCTCATT ATACCTCATT NNNNNNNNNN ATACCTCATT ATACCTCATT ACACTTCATT ACACCTCATT ACACTTCATT ATACCTCATT ATACTTCTTT ATACTTCATT ATACCTCATT ATACTTCATT ACACTTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACTTCATT ATACTTCATT ACACCTCATT ATACCTCATT ACACCTCATT ACACTTCATT ACACTTCATT ACACCTCATT ATACCTCATT ATACTTCATT ACACTTCATT ACACCTCCTT ATACCTCATT ATACATCATT ATACATCTTT ATACTTCATT ATACATCNTT ATACCTCATT ATACTTCATT ATACATCATT ATACTTCTTT ATACTTCTTT ATACCTCATT ATACTTCATT ATACTTCATT NNNNNNNNNN ACACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACTTCCTT aCACCTCNTT ....|....| 655 CTTTGATCCG CTTTGACCCA CTTTGATCCG CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGATCCC CTTTGACCCG CTTTGATCCA CTTTGATCCG CTTTGACCCA CTTTGACCCG TTTTGATCCT CTTTGATCCA TTTTGACCCA CTTTGACCCG TTTTGATCCA TTTCGATCCT TTTTGATCCG CTTTGATCCA CTTTGATCCG NNNNNNNNNN TTTTGACCCC CTTTGATCCT TTTTGATCCT CTTTGATCCG TTTTGATCCT TTTTGATCCT TTTTGATCCT TTTTGACCCT CTTTGATCCA TTTTGACCCT NTTTGATCCT TTTTGACCCC TTTtGATCCT TTTTGACCCC TTTTGATCCT TTTTGATCCT CTTTGATCCG TTTTGACCCC CTTTGATCCT TTTTGATCCT TTTTGATCCT CTTTGACCCG TTTTGACCCC TTTTGATCCA TTTTGATCCT TTTTGACCCT TTTTGATCCA CTTTGATCCT TTTTGATCCT TTTTGACCCT TTTTGACCCT TTTTGATCCA TTTTGATCCA TTTTGATCCA TTTTGATCCT NNNNNNNNNN TTTTGATCCT TTTTGATCCG TTTTGATCCA NNNNNNNNNN TTTTGATCCA CTTTGATCCG CTTCGATCCT CTTTGATCCA TTTTGACCCA CTTTGACCCG ....|....| 665 GCTGGTGGAG GCTGGAGGaG GCTGGTGGAG GCTGGTGGGG GCTGGTGGGG GCTGGTGGGG GCTGGTGNNN GCTGGCGGAG GCTGGAGGAG GCTGGTGGAG GCTGGTGGGG GCTGGTGGGG GCAGGAGGAG GCTGGTGGGG GCAGGTGGAG GCTGGCGGAG GCTGGCGGGG GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG GCTGGTGGAG NNNNNNNNNN GCTGGCGGAG GCAGGAGGAG GCAGGAGGAG GCTGGTGGAG GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG GCCGGAGGAG GCTGGAGGAG GCAGGAGGAG GCTGGAGGAG GCTGGCGGAG GcTGGAGGAG GCTGGAGGAG GCNGGNGGNG GCTGGAGGAG GCTGGTGGAG GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG GCTGGNGGAG GCTGGAGGAG GCAGGAGGAG GCTGGAGGAG GCAGGGGGAG GCTGGTGGGG GCTGGAGGAG GCAGGAGGAG GCTGGAGGAG GCAGGAGGAN GCTGGAGGAG GCAGGAGGAG GCAGGAGGAG GCTGGAGGAG NNNNNNNNNN GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG NNNNNNNNNN GCTGGTGGAG GCTGGTGGGG GCTGGTGGAG GCTGGTGGAG GCTGGGGGAG GCTGGTGGAG ....|....| 675 GAGATCCAAT GAGATCCAAT GAGATCCTAT GAGATCCTAT GAGATCCTAT GAGATCCTAT NNNNNNNNNN GAGATCCTAT GAGATCCAAT GAGATCCAAT GAGATCCTAT GAGATCCTAT GAGATCCAAT GAGATCCTAT GAGATCCTAT GAGATCCAAT GAGATCCNAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAN GAGATCCAAT GAGATCCNNN GAGATCCAAT GAGANNNNNN GAGANCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCTAT GAGATCCAAT GTGATCCAAT GGGATCCAAT GAGATCCAAT GTGACCCAAT GAGACCCAAT NNNNNNNNNN GTGATCCAAT GAGATCCTAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GNNNNNNNNN GaGATCCAAT ....|....| 685 TTTATATCAA TTTATACCAA TTTATATCAA TTTATATCAA TCTATACCAa TTTATANNNN NNNNNNNNNN TTTATATCAA TTTATATCAA TNNNNNNNNN TTNNNNNNNN TTNATANCAA CTTATATCAA TTTATATCAA TCTTTACCAA TTTATATCAA TTTATATCAA TTTATATCAN CTTANNNNNN TTTANNNNNN TTTATACCAA NNNNNNNNNN TTTATATCAA TTTATATCAC TTTATATCAA TTTATATCAA TCTATACCAA TTTATATCAA CTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAA NNNNNNNNNN TTTATATCAA NNNNNNNNNN TTTATATCAA NNNNNNNNNN TTNATATCAA TTTATATCAA TTTATATCAA TTTATATCAA TNTATATCAN TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCNN TTTATACCAA TTTATATCAA TTTATATCAN NNNNNNNNNN TTTATATCAA TTTATATCAA TTTATATCAA CTTATATCAA NNNNNNNNNN TTTATATCAA CTTATATCAA TTTATATCAA NNNNNNNNNN TTTATACCAA NNNNNNNNNN TNNNNNNNNN TTTATACCAA NNNNNNNNNN TTTATATCAA Appendix 3: Sequence data MW98089 MW98094 MW98097 MW98103 MW98106 MW98128 MW98129 MW98133 MW98136 MW98138 MW98139 MW98154 MW98162 MW98170 MW98172 MW98180 MW98183 MW98185 MW98189 MW98203 MW98205 MW98212 MW98220 MW98228 MW98230 MW98235 MW98240 MW98261 MW98264 MW98265 MW98270 MW98274 MW98278 MW98284 MW98285 MW98294 MW98313 MW98315 MW99001 MW99006 MW99009 MW99013 MW99014 MW99018 MW99028 MW99038 MW99042 MW99045 MW99047 MW99053 MW99057 MW99058 MW99059 MW99060 MW99061 MW99068 MW99080 MW99084 MW99094 MW99095 MW99097 MW99102 MW99104 MW99105 MW99124 MW99128 MW99134 MW99135 ....|....| 545 CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTGTTT TTATTTATTT CTATTTGTTT CTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT CTATTTATTT TTATTTATTT CTATTTGTTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT TTATTCATCT TTATTTATTT TTATTTATTT TTATTCATCT TTATTTATTT TTATTTATTT TTATTTGTTT TTATTTATTT CTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTaTTTaTTT TTATTTATTT TTATTCATCT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT CTATTTATTT CTATTTATTT CTATTTATTT TTATTTATCT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTaTTT TTATTTATTT ....|....| 555 GAGCTGTAGG GAGCTGTAGG GAGCTGTAGG GAGCGGTAGG GAGCAGTGGG GAGCGGTAGG GAGCGGTAGG GAGCAGTAGG GGGCTGTAGG GAGCTGTAGG GGGCTGTAGG GAGCCGTAGG GAGCTGTGGG GGGCTGTAGG GAGCAGTAGG GAGCTGTAGG GAGCTGTAGG GAGCGGTAGG GAGCTGTGGG GGGCTGTGGG GAGCAGTGGG GAGCAGTGGG GAGCAGTTGG GAGCAGTAGG GAGCTGTAGG GAGCGGTAGG GAGCAGTAGG GAGCAGTAGG GAGCGGTAGG GAGCTGTTGG GAGCTGTAGG GAGCAGTAGG GAGCAGTAGG GAGCTGTAGG GAGCTGTGGG GAGCAGTAGG GGGCTGTAGG GAGCAGTGGG GAGCAGTAGG GAGCTGTGGG GAGCAGTGGG GAGCAGTGGG GAGCGGTAGG GAGCaGTaGG GAGCAGTAGG GAGCGGTAGG GAGCGGTAGG GAGCAGTAGG GAGCAGTAGG GAGCGGTGGG GAGCTGTAGG GAGCTGTGGG GGGCTGTAGG GAGCAGTAGG GAGCTGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GGGCTGTAGG GAGCAGTGGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTCGG GAGCGGTAGG GAGCAGTAGG GAGCGGTAGG ....|....| 565 AATTACTGCA AATTACAGCT AATTACAGCA AATCACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACGGCA AATTACAGCA TATTACTGCT AATTACAGCA AATTACAGCT AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA TATTACAGCA TATTACTGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTaCAGCA AATTACAGCA AATTACAGCA AATTACAGCA TATTACTGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTaCTGcA TATTACTGCA AATTACAGCA AATTACAGCA TATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA TATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA TATTACTGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACTGCA AATTACAGCA TATTACTGCA AATTACAGCA Cytochrome Oxidase I (COI) ....|....| 575 TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAC TTATTATtAC TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTGTTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTGTTAC TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAT TTATTACTAC TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTaTTaTTaC TTATTATTAC TTATTATTAC TTATTATNNN TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAT TTATtACTTC TtATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTACTATTAC TTATTATTAC TTATTATTAC TTATTATTAC ....|....| 585 TTCTATCTTT TATtATCTTT TTCTATCTTT TTTTACCTTt TTTTATCTTt TTTTATCTTT TATTATCTTT TTTTATCTTT TTCTATCTTT TTCTATCTTT TTCTATCTTT TTCTATCTTT TTCTATCTTT TTCTATCTTT TTTTATCATT TTCTATCTTT TTCTATCTTT TACTATCTTT TTCTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCATT TTTTATCTTT TATTATCATT TATTATCTTT TCTTATCTTT TTTTATCTTT TTTTATCTTT TCTTATCTTT TATTATCTTT TTCTATCTTT TTTTATCTTT TATTATCTTT TTCTATCTTT TTCTATCTTT TTTTATCTTT TTCTATCTTT TTTTATCATT TTTTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTaTCTTT TTTTATCTTT TCTTATCTTT NNNNNNNNNN TATTATCTTT TTTTATCTTT TTTTATCTTT TTCTATCTTT TTCTATCTTT TTCTATCTTT TTTTATCTTT TTCTATCTTT TTTTATCTTT TACTATCTTT TTCTTTCTTT TTCTATCTTT TTTTATCTTT tTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTCT TTTTATCTTT TTTTATCTTT TTTTATCTTT ....|....| 595 ACCTGTATTA ACCTGTTTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCtGTATTA ACCTGTATTA ACCTGTATTA GCCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA GCCTGTATTA ACCTGTaTTA ACCTGTATTA ACCTGTATTA ACCTGTTTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTTTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA GCCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACcTGTATTA ACCTGTATTA AcCTGTATTA ACCTGTATTA NNNNNNNNNN ACCTGTTTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTTTTA ACCTGTTTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCAGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ....|....| 605 GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCTA GCAGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCAGGAGCAA GCAGGAGCAA GCTGGAGCTA GCTGGGGCAA GCTGGAGCTA GCGGGAGCAA GCTGGAGCaA GCAGGAGCAA GCTGGAGCTA GCTGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCGGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGcAA GCTGGAGCTA GCTGGAGCAA GCAGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCTA GCTGGaGCAA GCTGGAGCAA GCTGGAGCTA NNNNNNNNNN GCTGGTGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCAGGAGCAA GCTGGaGCAA GCAGGTGCTA GCTGGTGCTA GCTGGAGCTA GCTGGAGCAA GCAGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCCGGAGCAA GCTGGAGCTA GCGGGTGCAA GCTGGGGCAA ....|....| 615 TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACTATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACTATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACTATATT TTACTATATT TCACTATATT TTACTATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT NNNNNNNNNN TTACTATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACTATATT TTACTATANN TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACTATATT TTACCATATT ....|....| 625 ATTAACTGAT ATTAACTGAT ATTAACTGAT aTTAACCGAT ATTAACCGaT ACTAACTGAT ATTGACTGAT ATTAACGGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTGACTGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ACTAACTGAT ATTAACCGAT ATTAACAGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACCGaT ATTAACTGAT ATTAACAGAT ATTAACAGAT ATTAACTGAT ATTAACCGAT ATTAACTGAT ACTAACTGAT ATTAACTGAC ATTAACTGAT ATTAACTGAT NNNNNNNNNN ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACTGAT ATTAACCGAT ATTAACAGAT NNNNNNNNNN ATTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACCGAT ATTAACCGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT 181 ....|....| 635 CGAAACCTTA NNNNNNNNNN CGAAACCTTA CGAaACCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTCA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA CGAAATCtTA CGAAACCTTA CGTAATTTAA CGAAATTTAA CGAAATCTTA CGAAATCTTA CGAAATTTAA CGNAAtCTTA CGTAATCTTA CGAAACCTAA CGAAATCTCA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTa CGAAATCTTA CGAAATATTA CGAAACCTTA CGAAACCTTA CGAGATCTTA CGAAATCTTA CGAAACCTAA CGAAATATTA CGAAATTTAA NNNNNNNNNN CGAAACCTAA CGANNNNNNN CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA NNNNNNNNNN CGAAACCTTA CGAAATCTTA CGAAATATTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAATCTAA CGAAACCTTA CGAAACCTTA ....|....| 645 ATNCTTCCTT NNNNNNNNNN ACACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACTTCATT ATACCTCATT ATACCTCATT ATACCTCGTT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACTTCATT ATACCTCATT ATACCTCATT ACACCTCATT ACACCTCATT ATACTTCATT ATACTTCATT ATACTTCTTT ATACCTCATT ATACCTCATT ACACCTCATT ATACCTCATT ATACTTCNNN ATACCTCATT ATACCTCATT ACACTTCATT ATACCTCATT ATACCTCATT ACACCTCATT ATACCTCATT ACACCTCATT ATACCTCATT NNNNNNNNNN ATACCTCATT ATACCTCATT ATACCTCATT ATACTTCATT ATACCTCATT ATACCTCATT NNNNNNNNNN ATACTTCATT NNNNNNNNNN ACACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACCTCATT ATACCTCATT aNACNTCATT ATACCTCTTT NNNNNNNNNN ATACCTCATT ACACCTCATT ATACCTCATT ACACCTCATT ACACCTCGTT ACACCTCGTT ATACTTCATT ACACCTCATT ATACTTCGTT ATACCTCATT ....|....| 655 NTTTGACCCA NNNNNNNNNN CTTTGACCCA CTTTGACCCA CTTTGACCCA TTTTGATCCT TTTTGATCCT CTTTGATCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGATCCG CTTTGACCCA CTTTGACCCA TTTTGATCCT CTTTGACCCA CTTTGACCCA CTTTGATCCA CTTTGATCCA TTTTGATCCA TTTTGATCCT TTTTGATCCT TTTTGATCCT CTTTGACCCN CTTTGATCCA TTTTGATCCT NNNNNNNNNN TTTTGACCCT CTTTGATCCT TTTTGATCCT CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGATCCA TTTTGATCCT NNNNNNNNNN CTTTGATCCA TTTTGATCCt TTTTGATCCT CTTTGaTCCA TTTTGATCCT TTTTGATCCC NNNNNNNNNN TTTTGATCCT NNNNNNNNNN CTTTGATCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA TTTTGATCCA CTTTGACCCA CTTTGATCCG CTTTGATCCT NNNNNNNNNN CTTTGACCCA CTTTGATCCA TTTTGATCCT CTTTGATCCG CTTTGATCCG CTTTGANCCG TTTTGATCCA CTTTGATCCT TTTTGATCCG CTTTGATCCA ....|....| 665 GCTGGGGGAG NNNNNNNNNN GCTGGGGGAG GCTGGTGGAG GCTGGTGGAG GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG GCTGGGGGAG GCTGGAGGAG GCTGGGGGAG GCTGGGGGAG GCTGGAGGAG GCTGGTGGGG GCTGGGGGAG GCTGGGGGNN GCTGGAGGAG GCTGGNGGGG GCTGGAGGAG GCTGGTGGAG GCAGGTGGAG GCTGGAGGAG GCTGGAGGAG GCAGGAGGTG GCTGGAGGGG NNNNNNNNNN GCTGGTGGGG GCTGGAGGAG NNNNNNNNNN GCTGGAGGAG GCTGGTGGGG GCAGGAGGAG GCTGGGGGAG GCTGGGGGAG GNNNNNNNNN GCTGGAGGAG GCAGGNNNNN GCTGGAGGAG NNNNNNNNNN GCTGGTGGAG GCTGGTGGTG GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG GCTGGAGGGG NNNNNNNNNN GCTGGAGGAG NNNNNNNNNN GCNNNNNNNN GcTGGAGGAG GCTGGGGGAG GCTGGAGGAG GCTGGTGGAG GCTGGGGGAG GcTGGTGGAG GCTGGAGGAG NNNNNNNNNN GCTGGAGGAG GCTGGTGGAG GCTGGAGGAG GCTGGTGGAG GCTGGTGGAG GCTGGTNNNN GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG GCTGGNNNNN ....|....| 675 GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGATCCNNN GGGATCCAAT GAGATCCAAT GaGATCCAAT GAGATCNNNN GAGATCCAAT GAGATCCANN GAGATCCAAT GAGATCCAAT GAGANNNNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCANT GAGATCCAAT GTGATCCAAT GGGATCCNNN GAGATCCAAT GAGATCCTAT NNNNNNNNNN GAGATCCAAT GAGATCCTAT NNNNNNNNNN GAGATCCAAT GGGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCANN NNNNNNNNNN GAGATCCAAN NNNNNNNNNN GAGATCCAAT NNNNNNNNNN GAGATCNNNN GAGNNNNNNN GAGATCCAAT GAGANCCNNN GAGATCCTAT GAGATCCTAT NNNNNNNNNN GAGATCCAAT NNNNNNNNNN NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAN NNNNNNNNNN GAGATCCAAT GAGATCCAAT GGGATCCAAT NNNNNNNNNN ....|....| 685 TTTATATCAA NNNNNNNNNN TTTATACCAA NNNNNNNNNN TTTATACCAA TTTATATCAA TTTNNNNNNN NNNNNNNNNN TTTATACCAA NNNNNNNNNN TTTATACCAN TTTATANNNN NNNNNNNNNN TTTATACCAA TTTATATCAA TTTATATCAA NNNNNNNNNN TTTATATCAA TTTANNNNNN TTTATACCAA TTTATATCAA TTTANNNNNN TTTATATCAN NNNNNNNNNN TTTATACCAA TTTATATCAA NNNNNNNNNN TTTATACCAN TTTATATCAA NNNNNNNNNN TTTATATCAA TTTATATCAN CTTATATCAA NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CTTATATCAA NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TTTATATCAA NNNNNNNNNN TTTATATCAA TTTATATCAA NNNNNNNNNN TTTATATCAA NNNNNNNNNN NNNNNNNNNN TTTATACCAa TTTATACCAA TTTANNNNNN TTTNNNNNNN TTTNNNNNNN TTTATANNNN TCTATATCAA NNNNNNNNNN TTTATACCNN TTTATATCAA CTTATATCAA TTTATATCAA NNNNNNNNNN NNNNNNNNNN TTTATACNNN TTTATACCAA TTTATATCAA NNNNNNNNNN Appendix 3: Sequence data MW99140 MW99141 MW99158 MW99163 MW99164 MW99165 MW99170 MW99174 MW99187 MW99196 MW99203 MW99221 MW99226 MW99240 MW99247 MW99263 MW99264 MW99274 MW99276 MW99286 MW99289 MW99292 MW99301 MW99303 MW99309 MW99314 MW99319 MW99320 MW99341 MW99344 MW99353 MW99357 MW99372 MW99374 MW99376 MW99380 MW99381 MW99382 MW99393 MW99398 MW99406 MW99407 MW99408 MW99413 MW99422 MW99425 MW99430 MW99435 MW99448 MW99449 MW99465 MW99469 MW99471 MW99473 MW99476 MW99479 MW99494 MW99501 MW99508 MW99514 MW99515 MW99520 MW99526 MW99536 MW99537 MW99538 MW99546 MW99550 ....|....| 545 TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT NNNNNNNNNN TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATCT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT NNNNNNNNNN TTATTTATTT TTATTTATTT CTATTTATTT CTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT CTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTCATCT TTATTCATCT TTATTTATTT TTATTCATCT ....|....| 555 GAGCGGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTGGG GGGCTGTAGG GAGCAGTGGG GAGCAGTAGG NNNNNNNNNN GAGCAGTAGG GAGCGGTAGG GAGCTGTAGG GAGCGGTGGG GGGCTGTAGG GAGCTGTGGG GAGCAGTAGG GAGCAGTAGG GAGCAGTGGG GAGCAGTGGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTGGG GAGCGGTGGG GAGCAGTGGG GAGCAGTGGG GAGCAGTAGG GAGCGGtAGG GGGCTGTAGG GGGCTGTAGG GAGCGGTAGG GAGCGGTGGG GAGCAGTAGG GAGCAGTGGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG NNNNNNNNNN GAGCAGTAGG GAGCAGTAGG GAGCTGTAGG GAGCTGTGGG GAGCTGTGGG GAGCTGTAGG GGGCGGTAGG GAGCAGTAGG GGGCTGTAGG GGGCTGTAGG GGGCTGTAGG GAGCAGTAGG GAGCAGTGGG GAGCAGTAGG GAGCGGTGGG GAGCAGTGGG GAGCAGTAGG GAGCTGTAGG GAGCAGTGGG GAGCGGTAGG GAGCTGTAGG GAGCTGTAGG GAGCGGTAGG GAGCGGTAGG GAGCGGTAGG GAGCGGTAGG GAGCTGTAGG GAGCGGTAGG ....|....| 565 AATTACAGCA AATTACAGCA AATTACAGCa TATTACTGCT AATTACcGCA AATTACAGCA AATTACAGCA AATTACAGCA NNNNNNNNNN AATTACAGCA AATTACAGCA TATTACTGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA TATTACTGCT AATTACAGCA AATTACAGCA AATTACAGCA AATTACNNNN AATTACGGCA AATCACAGCA AATTACAGCA AATTACAGCA AATCACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA NNNNNNNNNN AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACTGCT TATTACTGCT AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCT AATTACAGCT AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA Cytochrome Oxidase I (COI) ....|....| 575 TTGTTATTAT TTATTATTAC TTATTATTAC TTGTTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC NNNNNNNNNN TTATTATTAC TTATTATTAC CTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTGTTAC TTATTATTAC TTATTATTAC TTATTATTAC NNNNNNNNNN TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC NNNNNNNNNN TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC CTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTGTTATTAT TTACTATTAC TTATTACTAT TTATTATTAC TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC ....|....| 585 TACTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT TTCTATCTTT TTTTATCTTT TTTTATCTTT NNNNNNNNNN TTTTATCTTT TTTTATCTTT GATTATCTTT TTTTATCTTT TTCTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT TTTTATCATT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT NNNNNNNNNN TTTTATCTTT TTTTATCTTT TTCTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT TTTTATCTTT TTTTATCTTT TTTTATCTTT NNNNNNNNNN TTTTATCTTT TTTTATCTTT TTCTATCTTT TTCTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TATTATCTTT TTCTATCTTT TTCTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCATT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT TTCTATCTTT TTTTATCTTT TACTATCTTT TATTATCTTT TACTATCTTT TTTTATCTTT TATTATCTTT TCTTATCTTT TCTTATCTTT TTTTATCCTT TCTTATCTTT ....|....| 595 ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTCTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA NNNNNNNNNN ACCTGTATTA ACCTGTATTA ACCAGTTTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA GCCTGTATTA GCCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTTTTA ACCTGTATTA ACCTGTATTA GCCTGTATTA NNNNNNNNNN ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA GCCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA NNNNNNNNNN ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCCGTATTA ACCTGTATTA GCCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA GCCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTTTTA ACCTGTTTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCCGTATTA ACCTGTATTA ....|....| 605 GCTGGAGCTA GCTGGAGCAA GCAGGTGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA NNNNNNNNNN GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCaA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA NNNNNNNNNN GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA NNNNNNNNNN GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCAGGAGCTA GCTGGAGCAA GCTGGAGCTA GCTGGAGCTA GCTGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCAGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGGGCTA GCTGGAGCTA GCTGGGGCAA GCTGGAGCTA GCtGGAGCTA GCTGGAGCTA GCTGGTGCAA GCTGGAGCTA ....|....| 615 TTACTATATT TTACTATATT TCACTATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT NNNNNNNNNN TTACCATATT TTACCATATT TTACTATATt TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACTATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT NNNNNNNNNN TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT NNNNNNNNNN TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACTATATT TTACTATATT TTACTATATT TTACCATATT TTACTATATT TTACTATATT TTACTATATT TTACCATATT TTACTATATT ....|....| 625 ATTAACTGAT ATTAACCGAT ATTAACTGAT ATTAACAGAT ATTAACAGAT ATTAACTGAT ATTAACCGAT ATTAACTGAT NNNNNNNNNN ATTAACCGAT ATTAACCGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACCGAt ATTAACAGAT ATTAACAGAT ATTAACCGAT ATtaaCCGAT ATTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACCGAT ATTAACTGAT ATTAACAGAT NNNNNNNNNN ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACTGAT ATTAACCGAT ATTAACAGAT ATTAACCGAT ATTAACCGAT ATTAACCGAT NNNNNNNNNN ATTAACCGAT ATTAACCGAT ATTAACTGaT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT 182 ....|....| 635 CGAAATCTTA CGAAACCTTA CGAAATTTAA CGAAATCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA NNNNNNNNNN CGAAATCTTA CGAAACCTTA CGAGATCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAGATCTTA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA NNNNNNNNNN CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA NNNNNNNNNN CGAAATCTTA CGAAATCTTA CGAAACCTCA CGAAACCTTA CGAAACCTTA CGTAATCTTA CGAAATCTTA CGAAATCTCA CGAAACCTTA CGAAACcTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAATCtTA CGTAATTTAA CGTAATTTAA CGAAACCTTA CGAAACCTTA CGAAATTTAA CGAAATTTAA CGAAATCTTA CGAAATTTAA ....|....| 645 ACACTTCATT ACACCTCGTT ATACTTCATT ATACTTCATT ACACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT NNNNNNNNNN ACACTTCATT ATACCTCNNN ATACTTCATT ACACCTCATT ATACCTCATT ATACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ATACCTCATT ATACCTCGTT ATACTTCATT ATACCTCATT ACACCTCATT ACACCTCATT NNNNNNNNNN ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT NNNNNNNNNN ACACCTCATT ANACNTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACATCATT ACACTTCATT ACACTTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCGTT ACACCTCATT ACACCTCATT ACACCTCATT ACACCTCATT ATACCTCATT ATACCTCATT ACACCTCATT ACACtTCATT ATACTTCTTT AtACTTCTTT ATACCTCATT ACACTTCATT ATACCTCATT ATACCTCATT ACACCTCATT ATACCTCATT ....|....| 655 TTTTGATCCT CTTTGATCCG TTTTGACCCT TTTTGATCCT CTTTGATCCA CTTTGACCCN CTTTGATCCA CTTTGACCCA NNNNNNNNNN CTTTGATCCG NNNNNNNNNN TTTTGACCCT CTTTGATCCA CTTTGACCCN CTTTGACCCA CTTTGATCCG CTTTGATCCG CTTTGATCCA CTTTGATCCA CTTTGATCCA TTTTGATCCA CTTTGATCCA TTTTGACCCA TTTTGATCCT CTTTGATCCG CTTTGATCCA CTTTGATCCA NNNNNNNNNN CTTTGATCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGATCCA CTTTGATCCA CTTTGACCCA CTTTGACCCA CTTTGACCCA CTTTGATCCA NNNNNNNNNN CTTTGATCCA CTTTGATCCG CTtTGACCCA CTTTGACCCA CTTTGACCCA TTTTGACCCT TTTTGATCCT TTTTGATCCT CTTTGACCCA CTTTGACCCA CTTTGACCCA TTTTGACCCA CTTTGATCCA CTTTGATCCA CTTTGATCCA CTTTGATCCA CTTTGATCCG CTTTGACCCA CTTTGACCCA TTTTGATCCT TTTTGATCCT TTTTGATCCT CTTTGATCCA TTTtGATCCT TTTTGATCCT TTTTGATCCT CTTTGATCCG TTTTGATCCT ....|....| 665 GCTGGAGGAG GCTGGTGGAG GCAGGAGGAG GCTGGAGGAG GCTGNNNNNN NNNNNNNNNN GCTGGTGGAG GCTGGTGGGG NNNNNNNNNN GCTGGTGGAN NNNNNNNNNN GCTGGAGGAG GCTGGTGGAG NNNNNNNNNN GCTGGGGGNN GCTGGTGGAG GCTGGTGGAG GCAGGTGGGG GCAGGTGGGG GCTGGNNNNN GCTGGTGGNN GCTGGTGGGG GCTGGCGGGG GCTGGAGGAG GCTGGTGGAG GCTGGTGGAG GCAGGTGGGG NNNNNNNNNN GCTGGTGGGG GCTGGTGGAG GCTGGGGGAG GCTGGGGGAG GCTGGTGGGG GCTGGTGGAG GCTGGTGGAG GCAGGTGGGG GCTGGtGGGG GCTGGTGGGG GCTGGTGGAG NNNNNNNNNN GCTGGTGGAG GCTGGTGGAG GCTGGGGGGG GCTGGGGGAG GCTGGGGGAG GCTGGAGGAG GCCGGAGGAG GCAGGAGGAG GCTGGAGGAG GCTGGAGGAG GCTGGAGGAG GCTGGCGGGG GCAGGTGGGG GCTGGTGGAG GCTGGTGGAG GCTGGTGGAG GCTGGTGGAG GCTGGGGGAG GCTGGTGGAG GCNGGAGGAG GCAGGAGGTG GCAGGTGGTG GCTGGTGGAG GCTGGAGGAG GCTGGAGGGG GCTGGAGGGG GCTGGAGGAG GCTGGAGGGG ....|....| 675 GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN NNNNNNNNNN GAGATCCAAT GAGATCCTAT NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GAGATCCAAT GAGATCCAAT NNNNNNNNNN NNNNNNNNNN GAGATCCAAT GNNNNNNNNN GAGATCCAAT GAGATCCAAT NNNNNNNNNN NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGANCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGANNNNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGATCCAAT GGGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCANN GAGATCCNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCNAT GAGATCNNNN GAGATCCAAT GAGATCCTAT GAGATCCTAT GaGATCCAAT GAGATCCTAT ....|....| 685 TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAN NNNNNNNNNN NNNNNNNNNN TTTANNNNNN TTTATATCAn NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TTTATATCAA TTTATATCAN NNNNNNNNNN NNNNNNNNNN TTNNNNNNNN NNNNNNNNNN TTTATATCAA TTTNNNNNNN NNNNNNNNNN NNNNNNNNNN TTTATACCAA TTTATACCAA TTTATATCAN TTTATATCAA TTTATATCAA TTTATATCAA NNNNNNNNNN TTTATACCAA NNNNNNNNNN TTTATACCAN TTTATACCAN NNNNNNNNNN TTTANNNNNN TTTATACCAA TTTATATCAA TTTNNNNNNN TTTATATCAA TTTATACCAA NNNNNNNNNN TTTATACCAN TTTATANNNN NNNNNNNNNN TTTATACCAA TTTATACCAA TTTATATCAA TTNNNNNNNN CTTATATCAA TTTATACCAA TTTATACCAA TTTATACCAA TTTATNNNNN NNNNNNNNNN TTTATACCAA TTTATATCAA NNNNNNNNNN NNNNNNNNNN TTTATATCAA TTTATATCAA TTTANNNNNN TTTATATCAA TTTATATCAA NNNNNNNNNN TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAA TTTATATCAA Appendix 3: Sequence data MW99552 MW99559 MW99565 MW99579 MW99591 MW99605 MW99608 MW99612 MW99613 OK96022 OK99001 WE00002 WE00003 WE02321 WE02421 WE02431 WE02451 WE02491 WE02531 WE02535 WE02536 WE02591 WE02612 WE02614 WE02621 WE02661 WE02662 WE02671 WE02674 WE02676 WE02677 WE85001 WE98001 ....|....| 545 TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT CTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT TTATTTATTT ....|....| 555 GAGCGGTGGG GAGCAGTGGG GAGCGGTAGG GAGCAGTAGG GAGCAGTGGG GAGCAGTGGG GAGCAGTAGG GAGCGGTAGG GAGCTGTGGG GAGCGGTAGG GAGCGGTAGG GAGCAGTAGG GAGCAGTAGG GGGCTGTAGG GGGCTGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG GGGCAGTAGG GGGCTGTAGG GAGCAGTGGG GAGCAGTGGG GAGCAGTGGG GGGCTGTAGG GAGCAGTAGG GAGCAGTGGG GAGCGGTAGG GGGCTGTAGG GAGCAGTGGG GAGCAGTAGG GAGCAGTAGG GAGCAGTAGG Cytochrome Oxidase I (COI) ....|....| 565 AATTACAGCA AATTACAGCA AATCACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATtACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACAGCA AATTACGGCA AATTACAGCA AATTACAGCA AATTACGGCA AATTACGGCA AATTACGGCA AATTACAGCA AATTACGGCA AATTACAGCA AATTACGGCA AATTACAGCA AATTACGGCA AATTACAGCA AATTACAGCA AATTACAGCA ....|....| 575 TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAT TTATTATTAT TTATTATTAC TTGTTATTAT TTATTATTAT TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC TTATTATTAC ....|....| 585 TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCATT TTTTATCATT TATTATCTTT TATTATCTTT TTTTATCCTT TATTATCTTT TATTATCTTT TTTTATCTTT TTTTATCTCT TTCTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT ....|....| 595 ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA GCCCGTATTA GCCCGTATTA ACCTGTATTA ACCTGTATTA ACCCGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA GCCTGTATTA ACCAGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTGTTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTATTA ACCTGTGTTA ACCTGTATTA ....|....| 605 GCTGGAGCAA GCTGGAGCAA GCAGGAGCAA GCTGGAGCAA GCTGGGGCAA GCTGGGGCAA GCTGGAGCTA GCTGGAGCTA GCTGGTGCAA GCTGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCTA GCTGGAGCTA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCTA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA GCTGGAGCAA ....|....| 615 TTACCATATT TTACCATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACTATATT TTACTATaTT TTACCATATT TTACTATATT TTACTATATT TTACCATATT TTACTATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT TTACCATATT ....|....| 625 ATTAACTGAT ATTAACCGAT ATTAACCGAT ATTAACTGAT ATTAACAGAT ATTAACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ACTAACTGAT ATTAACTGAT ATTGACAGAT ATTAACTGAT ATTAACTGAT ATTAACTGAT ATTAACCGAT ATTAACTGAT ATTAACTGAT ATTAACCGAC ATTAACCGAT ATTAACTGAT ATTAACCGAT ATTAACCGAT ATTAACCGAT ATTAACTGAT ATTAACCGAT ATTAACCGAT ATTAACCGAT ATTAACCGAT ATTAACCGAT ATTAACCGAT ATTAACTGAT ATTAACAGAT ....|....| 635 CGAAATCTTA CGAAaTCTTA CGAAACCTTA CGAAACCTCA CGAAATCTTA CGAAATCTTA CGAAATCTCA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAATCTTA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA CGAAATCTTA CGAAACCTTA CGAAACCTTA ....|....| 645 ACACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACTTCATT ACACTTCATT ACACCTCATT ACACTTCATT ACACTTCATT ATACTTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACTTCATT ATACCTCATT ACACCTCATT ACACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ATACCTCATT ACACCTCATT ATACCTCATT ACACCTCATT ATACCTCATT ACACCTCATT ACACCTCATT ATACCTCATT ATACCTCATT ....|....| 655 CTTTGATCCA CTTTGATCCG CTTTGATCCA CtTTGATCCA CTTTGACCCG CTTTGACCCG TTTTGATCCT TTTTGATCCT CTTTGATCCG TTTTGATCCT TTTTGATCCT CTTTGATCCG NNNNNNNNNN CTTTGACCCA CTTTGACCCA CTTTGACCCG CTTTGATCCC CTTTGATCCA CTTTGATCCA CTTTGATCCG CTTTGACCCA TTTTGATCCA TTTTGATCCA TTTTGATCCA CTTTGACCCA CTTTGATCCA CTTTGACCCG CTTTGATCCA CTTTGACCCA CTTTGATCCG CTTTGACCCA CTTTGATCCA CTTTGACCCA ....|....| 665 GCTGGTGGAG GCTGGTGGAG GCTGGTGGAG GCTGGAGGAG GCTGGTGGAG GCTGGTGGAG GCAGGAGGAG GCTGGAGGAG GCTGGANNNN GCTGGAGGAG GCTGGAGGAG GCTGGTGGGG NNNNNNNNNN GCTGGGGGAG GCTGGAGGAG GCTGGTGGAG GCTGGTGGAG GCTGGAGGAG GCTGGTGGAG GCTGGTGGAG GCTGGGGGGG GCTGGTGGAG GCTGGTGGAG GCTGGTGGAG GCTGGAGGAG GCTGGTGGAG GCTGGTGGAG GCTGGTGGAG GCTGGAGGAG GCTGGTGGGG GCTGGTGGGG GCTGGAGGAG GCTGGTGGAG ....|....| 675 GAGATCCAAT GAGATCCAaT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCNNNN GAGATCCAAT GAGATCNNNN NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCTAT NNNNNNNNNN GAGATCCAAT GAGATCCAAT GAGATCCAAT GGGATCCTAT GAGATCCTAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAN GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCCAAT GAGATCNNNN GAGATCCAAT ....|....| 685 TTTATATCAA TTTATATNNN TTTATACCAA TTTATATCAN TTTATATCAA NNNNNNNNNN CTTATATCAA NNNNNNNNNN NNNNNNNNNN TTTATATCAA TTTATATNNN TCNNNNNNNN NNNNNNNNNN TTTATACCAA TTCAATNNNN TTTATATCAN TTTATATCAA TTTNNNNNNN TTTATACCNN TTNNNNNNNN TNNNNNNNNN TTTATACNNN TTTATACCAA TTNNNNNNNN TTTNNNNNNN NNNNNNNNNN TTTATATCAA TTTATACCAA TTTATACCAA TTTATACCAA TTNNNNNNNN NNNNNNNNNN TTTACATCAA Cytochrome b (Cytb) MW98006 MW98055 MW98102 MW98225 MW99258 ....|....| 5 ATTATTACAG ATTATCACAG ATTATCACAG ATTTTTACAG ATTATCACAG ....|....| 15 GATTATTTTT GATTATTTTT GATTATTTTT GATTATTTTT GATTATTTTT ....|....| 25 AACTATATAT GACTATGTAC GACTATATAT AACTATATAT AACTATGTAC ....|....| 35 TATTCTGCTA TACTCAGCTA TATTCAGCTA TACTCAGCTA TATTCAGCTA ....|....| 45 ATATTAATTT ATATTAATTT ATATTAATTT ATATTAATTT ATATTAATTT ....|....| 55 AGCTTTTTAT AGCATTTTTT AGCATTTTTT AGCATTTTTC AGCATTTTTT ....|....| 65 AGTGTTAATT AGAGTAAATT AGAGTAAATT AGAGTAAATT AGAGTAAATT ....|....| 75 ACATTTGTCG ATATTTGTCG ATATTTGTCG ATATCTGCCG ACATTTGTCG ....|....| 85 AGATGTTAAT AGATGTAAAT AGATGTAAAT AGATGTAAAT AGATGTAAAT ....|....| 95 TATGGTTGAT TATGGATGAT TACGGATGAT TATGGATGAC ?ATGGATGAT ....|....| 105 TAATTCGAAC TAATTCGAAC TAATTCGAAC TAATTCGAAC TAATTCGAAC ....|....| 115 TCTTCATGCC TTTACATGCT TTTACATGCT TTTACATGCC TTTACACGCT ....|....| 125 AATGGAGCAT AATGGGGCTT AATGGAGCTT AATGGtGCTT Aa?GGAGCTT ....|....| 135 CTTTTTTTTT CATTTTTTTT CTTTTTTTTT CATTTTtCTT CATTTTTTTT MW98006 MW98055 MW98102 MW98225 MW99258 ....|....| 145 TATTTGtATT TGTTTGTATT TGTTTGTATT TATTTGCATT TGTTTGTATT ....|....| 155 TtCACtCATA TATACTCATA TATATTCATA TATATTCATA TATATTCATA ....|....| 165 TtGGACgAGG TTGGACGAGG TtGGACGAGG TtGGACGAGG TTGGACGAGG ....|....| 175 AATTTATtAt AATTTATTAT AATTTATTAT AATTTATCAT AATTTATTAT ....|....| 185 gAATCATtCGAATCCTTTGAATCCTTTGAATCATTCA GAATCCTTT- ....|....| 195 --AATTtAAA --AACCTAAA --AACTtAAA TTAATTTAAA --AaCTtAAA ....|....| 205 ACTTACAtGA AATAACATGA AAtAACATGA ATtAACATGA AATAACATGA ....|....| 215 AtAATTGGTG ATAATCGGAG ATAATCGGAG ATAATTGGAG AtAATCGGAG ....|....| 225 tAATtATTTT TATTGATTTT TATTAATTTt TGTtAAtCtt TATTAATTTT ....|....| 235 ATTTATATTA ATTTATATTG ATTtATATtA AtTtAtAtTA ATTTATaTTA ....|....| 245 ATAGCAACAg ATAGCAACAG ATAGCAACAG AtAGCAACaG ATAGCAACAG ....|....| 255 CATTtATAGG CTTTtATAGG CTTTTATAGG CTTTTATAGG CTTTTAtGGG ....|....| 265 ATATGTCCTA AtATGTTTTA AtAtgTTCTN ATATGTTTTA ATACGTTTTA ....|.. 275 CCTGGNN CCTTNNN CCCTNNN CCTTGAG CCTTNNN 183 Appendix 3: Sequence data NADH dehydrogenase subunit 1 (ND1) JC00057 MW00032 MW00056 MW00072 MW00076 MW00189 MW00231 MW00302 MW00328 MW00409 MW00412 MW00497 MW00530 MW98154 MW98172 MW98228 MW99014 MW99045 MW99068 MW99105 MW99135 MW99164 MW99406 MW99471 MW99550 ....|....| 5 TTAGCTTTTT TTAGCTTTTT TTAGCTTTTT TTAGCTTTTT TTAGCTTTTT TTGGCTTTTT TTAGCTTTTT TTAGCTTTTT TTAGCTTTTT TTAGCTtTTT TTAGCTTTTT TTAGCATTTT TTAGCTTTTT TTAGCTTTTT TTAGCTTTTT CTACCTTTTT TTaGCTTTTT TTAGCTtTTT TTGGCCTTTT TTGGCCTTTT TTAGCTTTTT TTAGCTttTT TTAGCTTTTT TTAGCTTTTT TTAGCTTTTT ....|....| 15 TAACATTAAT TAACATTAAT TAACATTAAT TAACACTAAT TAACATTAAT TGACACTAAT TGACATTAAT TAACATTAAT TAACATTAAT TAACATTAAT TAACATTAAT TAACTTTATT TAACATTAAT TAACATTAAT TGACATTAAT TGACATTAAT TAACATTAAT TAACATTAAT TAACATTAAT TGACACTAAT TAACATTAAT TGACATTAAT TAACATTAAT TGACATTAAT TAACGTTAAT ....|....| 25 AGAACGAAAA AGAACGAAAA AGAACGAAAA ASAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAG AGAACGAAAA AGAACGAAAA AGAACGAAAA GGAGCGGAAG AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAA AGAACGAAAG ....|....| 35 GTTTTAAGAT GTTTTGAGAT GTTTTAAGAT GTTTTGAGAT GTTTTAAGAT GTTTTAAGAT GTTTTAAGAT GTTTTAAGAT GTTTTGAGAT GTTTTAAGAT GTTTTAAGAT GTTTTAAGAT GTTTTAAGAT GTTTTGAGAT GTTTTAAGAT GTTTTAAGAT GTTTTAAGAT GTTTTAAGAT GTTTTAAGAT GTTTTAAGAT GTTTTGAGAT GTTTTAAGAT GTTTTAAGAT GTTTTAAGAT GTTTTAAGAT ....|....| 45 ATATACAAAT ATATACAAAT ACATACAAAT ATATTCAAAT ATATACAAAT ACATGCAAAT ACATACAAAT ATATGCAAAT ATATACAAAT ATATACAAAT ATATACAAAT ATATACAAAT ATATACAAAT ATATGCAGAT ACATACAAAT ATATACAAAT ACATACAAAT ATATACAAAT ACATGCAAAT ACATGCAAAT ATATACAAAT ATATGCAAAT ATATACAAAT ACATACAAAT ATATACAAAT ....|....| 55 TCGTAAAGGA TCGTAAAGGA TCGTAAAGGA TCGTAAAGGG TCGTAAAGGG TCGTAAAGGT TCGTAAAGGA TCGTAAGGGA TCGTAAAGGA TCGTAAAGGG TCGTAAAGGG TCGAAAAGGT TCGTAAAGGG TCGTAAAGGG TCGTAAAGGG TCGTAAAGGT TCGTAAAGGA TCGTAAAGGA TCGCAAAGGT TCGTAAAGGT TCGAAAAGGG TCGTAAAGGA TCGTAAAGGA TCGTAAAGGA TCGTAAGGGG ....|....| 65 CCTAATAAAG CCAAATAAGG CCAAATAAGG CCAAATAAGG CCAAATAAGG CCAAATAAGG CCGAATAAGG CCAAATAAGG CCAAATAAGG CCAAATAAGG CCAAATAAGG CCTAATAAAA CCAAATAAGG CCGAATAAGG CCAAATAAGG CCTAATAAGG CCAAATAAAG CCTAATAAAA CCAAATAAGG CCAAATAAGG CCAAATAAGG CCAAATAAGG CCAAATAAGG CCAAATAAGG CCTAATAAGG ....|....| 75 TTGGGTTTTT TTGGATTTTT TTGGATTtTT TTGGATTTTT TTGGATTTTT TTGGATTTTT TTGGGTTTTT TTGGATTTTT TTGGATTTTT TTGGATTTTT TTGGATTTTT TAGGGTTTAT TTGGATTTTT TTGGATTTTT TTGGATTTTT TTGGATTTTT TTGGATTTTT TTGGATTTTT TTGGATTTTT TTGGATTTTT TTGGGTTTTT TTGGGTTTTT TTGGGTTTTT TTGGGTTtTT TTGGGTTTTT ....|....| 85 AGGAATATTA AGGAGTATTA AGGGATATTA AGGGATATTA GGGAATATTA AGGGTTGTTA AGGAATATTA AGGGATATTA AGGGATATTA AGGAATATTA GGGTATATTA AGGAATGTTA AGGTATATTA AGGAATATTA AGGAATATTA AGGGATATTA AGGAATATTA AGGTATATTA AGGGTTATTA AGGGTTATTA AGGAATATTA AGGAATATTA AGGGATATTG AGGGATATTA AGGAATATTA ....|....| 95 CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAGCCTTTTT CAGCCTTTTT CAGCCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCATTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT CAACCTTTTT ....|....| 105 CTGATGGTAT CTGATGGTAT CTGATGGTAT CAGACGGTAT CAGATGGTAT CGGATGGTGT CGGATGGTAT CTGATGGTAT CTGACGGTGT CAGATGGTGT CAGACGGTGT CTGATGGAAT CAGATGGTGT CAGATGGTAT CGGATGGTAT CAGATGGTAT CAGATGGTGT CTGATGGTAT CGGATGGTAT CGGATGGTGT CTGACGGTAT CGGATGGTAT CTGATGGTAT CGGATGGTAT CAGATGGTGT JC00057 MW00032 MW00056 MW00072 MW00076 MW00189 MW00231 MW00302 MW00328 MW00409 MW00412 MW00497 MW00530 MW98154 MW98172 MW98228 MW99014 MW99045 MW99068 MW99105 MW99135 MW99164 MW99406 MW99471 MW99550 ....|....| 125 ACTAAAGAAA ACTAAAGAGA ACTAAAGAGA ACTAAAGAAA ACTAAAGAAA ACTAAAGAAA ACTAAAGAAA ACTAAAGAAA ACTAAAGAAA ACTAAAGAGA ACTAAAGAAA ACAAAAGAAA ACTAAAGAAA ACTAAAGAAA ACTAAAGAAA ACAAAAGAAA ACTAAAGAAA ACAAAAGAAA ACTAAGGAAA ACTAAAGAAA TCTAAAGAAA ACTAAAGAAA ACTAAAGAGA ACTAAAGAAA ACTAAAGAGA ....|....| 135 TGATTTATTT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATCT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATCT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATTT TAATTTATCT TAATTTATTT TAATTTATCT TAATTTATTT ....|....| 145 AAATTCATCA AAATTCTTCA AAATTTTTCA AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTCTTCT AAATTCTTCA AAATTCTTCT AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTCATCA AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTCTTCA AAATTTTTCA ....|....| 155 AATTATATAT AATTATATAT AATTATATAT AATTATATGT AATTATATAT AATTATATGT AATTATATGT AATTATATGT AATTATATAT AATTATATAT AATTATATAT AATTATTTAT AATTATATAT AATTATATAT AATTATATGT AATTATATAT AATTATATAT AATTATTTAT AATTATATGT AATTATATGT AATTATATAT AATTATATGT AACTATATAT AATTATATGT AATTATATAT ....|....| 165 TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTATTATTT TTTACTATTT TTTATTATTT ....|....| 175 GTCTCCTATT ATCTCCTATT GTCCCCTATT ATCTCCTATT ATCTCCTATT ATCCCCTATT ATCTCCTATT ATCTCCTATT GTCTCCTATT AtCCCCTATT TTCTCCTATT AGCTCCAGTT TTCTCCTATT GTCTCCTATT ATCTCCTATT ATCTCCTATT ATCTCCTATT ATCTCCAATT ATCTCCTATT ATCCCCTATT ATCTCCTATT ATCTCCTATT GTCCCCTATT ATCTCCTATT ATCTCCTATT ....|....| 185 ATAGGTTTTA GTAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTtA ATAGGTTTTA ATTGGATTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGATTTA GTAGGTTTTA ATTGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ATAGGTTTTA ....|....| 195 TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTtATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TATTATCTTT TTTTATCTTT TTTTATCTTT TTCTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCTTT TTTTATCCTT ....|....| 205 AATAATTTGA AGTGATTTGA AGTGATTTGA AGTGGTTTGA GGTAATTTGA AGTGATTTGA GGTGATTTGA AGTGATTTGA AGTGATTTGA aGTAATTTGA AGTGATTTGA AATGGTTTGA AGTGATTTGA AGTGATTTGA GGTGATTTGA AGTAATTTGA AGTGGTTTGA AATATTATGA AGTGATTTGA AGTGATTTGA GGTAATTTGA GGTGATTTGA AGTGATTTGA GGTGATTTGA GGTGATTTGA ....|....| 215 ATATTAATTC ATATTAATTC ATGTTAATTC ATATTAATTC ATGTTAATTC ATATTAATTC ATATTAATTC ATGTTAATTC ATGTTAATTC ATATTAATTC ACATTAATTC ATATTAATTC ATATTAATTC ATATTAATTC ATATTAATTC ATGTTAATTC ATATTAATTC ATATTAATTC ATATTAATTC ATATTAATTC GTGTTAATTC ATATTAATTC ATGTTAATTC ATATTAATTC ATATTAATTC ....|....| 225 CTTATTATTT CTTATTATTT CTTATTATTT CTTATTATTT CTTATTGTTT CTTATTATTT CTTATTATTT CTTATTATTT CTTATTATTT CTtATTATTT CTTATTATTT CTTACTATTT CTTATTATTT CTTATTATTT CTTATTATTT CTTATTATTT CTTATTATTT CATATTATTT CTTATTATTT CTTATTATTT CTTATTATTT CTTATTATTT CTTATTATTT CTTATTATTT CTTATTATTT 184 ....|....| 115 TAAATTATTT TAAATTATTT TAAATtATTT TAAATTATTT TAAATTATTT TAAATTATTT TAAATTATTT TAAATTATTT TAAATTATTT TAAATTATTT TAAATTGTTC TAAGTTATTT TAAATTGTTC TAAGTTATTT TAAATTATTT TAAGTTATTT TAAATTATTT TAAATTATTC TAAATTATTT TAAATTATTT TAAATTATTT TAAATTATTT TAAATTATTT TAAATTATTT AAAACTATTT Appendix 3: Sequence data AD98001 AD98009 AD98012 AD98018 AD98024 AD98036 DS00001 DS01001 JC00029 JC00039 JC00040 JC00042 JC00043 JC00045 JC00051 JC00055 JC00061 JC00063 JC01014 JM00001 MW00015 MW00024 MW00032 MW00051 MW00058 MW00064 MW00072 MW00076 MW00101 MW00102 MW00110 MW00116 MW00127 MW00129 MW00151 MW00176 MW00177 MW00178 MW00179 MW00185 MW00189 MW00226 MW00231 MW00232 MW00234 MW00262 MW00267 MW00269 MW00290 MW00302 MW00316 MW00326 MW00328 MW00330 MW00347 MW00348 MW00393 MW00409 MW00412 MW00444 MW00452 MW00469 MW00498 MW00504 MW00517 MW00525 MW00530 MW00539 ....|....| 5 TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNNNGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNNNNNGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNTAGTGTG TCATAGTGTG NNATAGTGTG NNATAGTGTG NNATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNNNNNNNG NNNTAGTGTG TCATAGTGTG T~ATAGTGTG T~ATASTGTG T~ATAGTGTG NNNNNNTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNTAGTGTG TYATAGTGTG NNNNNNNNTG TCATAGTGTG ....|....| 15 AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AANTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA ....|....| 25 CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATKTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA Internal transcribed spacer 2 (ITS-2) ....|....| 35 CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT ....|....| 45 TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC ....|....| 55 ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ....|....| 65 GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC ....|....| 75 ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ....|....| 85 ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTYT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT 185 ....|....| 95 GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC ....|....| 105 TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAATT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT ....|....| 115 CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATGTGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATATGCCAC CATATGCCAC AATATGCCAC CATATGCCAC CATATGCCAC ....|....| 125 ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTYGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ATTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ....|....| 135 ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~CT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~~~~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~~~~ ~T~~~~~GT~ ~C~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ GTC~~~~GTC ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GC~ ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ GTC~~~~GTC ~T~~~~~GT~ ~T~~~~~GT~ GT~~~~~AT~ ~T~~~~~~~~ ~T~~~~~GT~ ~T~~~~~GT~ ....|....| 145 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ GTCGTCT~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ....|....| 155 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ Appendix 3: Sequence data MW00547 MW01001 MW01011 MW01014 MW01018 MW01019 MW01025 MW01034 MW01039 MW01048 MW01053 MW01059 MW01061 MW01083 MW01092 MW01107 MW01116 MW02001 MW02006 MW02025 MW02033 MW02034 MW98009 MW98029 MW98049 MW98079 MW98097 MW98103 MW98129 MW98136 MW98138 MW98139 MW98154 MW98162 MW98170 MW98172 MW98180 MW98185 MW98189 MW98203 MW98205 MW98212 MW98220 MW98228 MW98235 MW98240 MW98261 MW98264 MW98270 MW98278 MW98285 MW98294 MW98313 MW98315 MW99001 MW99006 MW99009 MW99013 MW99014 MW99018 MW99038 MW99047 MW99057 MW99058 MW99094 MW99095 MW99097 MW99105 ....|....| 5 TCATAGTGTG NNNNNNNGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TC~TAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNNNNTGCG TCATAGTGTG NNNNNNNNNN TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNTAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTA NNNNNNNGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNTAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG T~ATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TC~TAGTGTG TC~TAGTGTG TCATAGTGTG NNNNNNNNNG NNNNNNNNNN TCATAGTGTG TCATAGTGTG NNATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG T~ATAGTGTG NC~TAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TMATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG ....|....| 15 AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA NNNNNNNNNN AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA ....|....| 25 CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA NNNNNNNNNN CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA Internal transcribed spacer 2 (ITS-2) ....|....| 35 CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT NNNNNACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT ....|....| 45 TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCSAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC ....|....| 55 ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ....|....| 65 GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC ....|....| 75 ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ....|....| 85 ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTTT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT 186 ....|....| 95 GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGG~CCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC ....|....| 105 TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAC TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT ....|....| 115 CATATGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATATGCCAC AATATGCCAC CATATGCCAC AATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATGTGCCAC CATATGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATGTGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATGTGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC ....|....| 125 ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ATTGTTCGTG ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ATTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGCC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTMGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ATTGTTCGTG ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ....|....| 135 ~T~~~~~GT~ ~T~~~~~GT~ GTC~~~~GTC ~T~~~~~GT~ GTC~~~~GTC ~CCGACTGT~ ~T~~~~~GT~ GTC~~~~GTC ~T~~~~~GT~ ~T~~~~~~~~ ~T~~~~~GT~ GTC~~~~GTC ~T~~~~~~~~ ~T~GAC~GTT GTC~~~~GTC ~T~~~~~GT~ GTC~~~~GTC ~T~~~~~GT~ ~T~~~~~GT~ GTC~~~~~~~ ~T~~~~~~~~ ~TC~~~~~~~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ GTC~~~~GTC ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ GTC~~~~GTC ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~~~~~~~GTC GTC~~~~GTC ~T~~~~~GT~ ~C~~~~~GC~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~~~~ GTC~~~~GTC ~T~~~~~GT~ ~C~~~~~GC~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~~~~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~~~~ ~C~~~C~GT~ ~T~~~~~~~~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~T~~~~~~~~ ~T~~~~~GT~ ....|....| 145 ~~~~~~~~~~ ~~~~~~~~~~ GTCGTC~T~~ ~~~~~~~~~~ GTCGTC~T~~ ~~~~~~~~~~ ~~~~~~~~~~ GTCGTCGTC~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ GTCGTC~T~~ ~~~~~~~~~~ ~~~~~~~~~~ GTCGTC~T~~ ~~~~~~~~~~ GTCGTC~T~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ GTCGTCGTCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ GTCGTCGTC~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ GTCGTCGTCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ GTCGTCN~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ....|....| 155 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TCGTCGTCTC ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TC~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TCGTCGTCTC ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ Appendix 3: Sequence data MW99134 MW99135 MW99163 MW99164 MW99174 MW99196 MW99203 MW99226 MW99240 MW99247 MW99274 MW99276 MW99286 MW99292 MW99301 MW99303 MW99309 MW99319 MW99341 MW99353 MW99372 MW99374 MW99382 MW99393 MW99406 MW99407 MW99408 MW99413 MW99448 MW99465 MW99471 MW99479 MW99501 MW99508 MW99514 MW99536 MW99537 MW99546 MW99550 MW99552 MW99559 MW99565 MW99591 MW99605 MW99608 MW99612 MW99613 OK96022 WE00002 WE02431 WE02451 WE02454 WE02491 WE02531 WE02534 WE02535 WE02591 WE02614 WE02621 WE02661 WE02662 WE02671 WE02674 WE02677 WE85001 WE94001 ....|....| 5 T~ATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TC~TAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNNNNNNNN TCATAGTGTG TCATAGNGTG T~TTAGTGGG T~ATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNNNNNNNN TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNNNNNNNG TC~TAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TC~TAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG CTATAGTGTG TCATAGTGTG TC~TAGTGTG NNNNNNNNNN TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNNNNNNNN TCATAGTGTG TCATAGTGTG T~ATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG NNNNNNTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG T~ATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG TCATAGTGTG ....|....| 15 AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA NNNNNNNNNA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA NNNNNNNNNN AACTGCAGGA AACTGCAGGA AACTG~AGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA NNNNNNNNNN AACTGCAGGA AACTGCAGGA MACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGGA AACTGCAGAA AACTGCAGGA AACTGCAGGA ....|....| 25 CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTWGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA NNNNNNNNNA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA NNNNNNNNNN CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA CACATTTGAA Internal transcribed spacer 2 (ITS-2) ....|....| 35 CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCTACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT NNNNNNNNNN CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT CATCGACATT ....|....| 45 TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCSAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC NCNAACGCNC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC NNNNNNNNNN TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC TCGAACGCAC ....|....| 55 ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC NNNGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ATTGCGGTCC ....|....| 65 GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC GTGGAGAAAC ....|....| 75 ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCANGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ATCCAGGACC ....|....| 85 ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTTT ACTCCTGTCT ACTCCTGTTT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT ACTCCTGTCT 187 ....|....| 95 GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCNGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC GAGGGCCGGC ....|....| 105 TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TG~GTAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAAT TGTATAAAGT ....|....| 115 CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATACGCCAC CATACGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATACGCCAC CATACGCCAC CATATGCCAC CATACGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATATGCCAC CATGTGCCAC ....|....| 125 ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTNGTN ACTGTTCGTC ACTGTTGGTN ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ACTGTTCGTC ....|....| 135 ~T~~~~~~~~ ~T~~~~~GT~ ~T~~~~~~~~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~T~~~~~~~~ ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ GTC~~~~GTC GTC~~~~GTC ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ GTC~~~~GTC GTN~~~~GTG ~T~~~~~GT~ GTN~~~~GTG ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~C~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ~T~~~~~GT~ ....|....| 145 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ GTCGTCGTCG GTCGTCGTCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ GTCGTCT~~~ GTCGTC~T~~ ~~~~~~~~~~ GTCGTC~T~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ....|....| 155 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TC~~~~~~TC TCT~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ Appendix 3: Sequence data AD98001 AD98009 AD98012 AD98018 AD98024 AD98036 DS00001 DS01001 JC00029 JC00039 JC00040 JC00042 JC00043 JC00045 JC00051 JC00055 JC00061 JC00063 JC01014 JM00001 MW00015 MW00024 MW00032 MW00051 MW00058 MW00064 MW00072 MW00076 MW00101 MW00102 MW00110 MW00116 MW00127 MW00129 MW00151 MW00176 MW00177 MW00178 MW00179 MW00185 MW00189 MW00226 MW00231 MW00232 MW00234 MW00262 MW00267 MW00269 MW00290 MW00302 MW00316 MW00326 MW00328 MW00330 MW00347 MW00348 MW00393 MW00409 MW00412 MW00444 MW00452 MW00469 MW00498 MW00504 MW00517 MW00525 MW00530 MW00539 ....|....| 165 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ....|....| 175 ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~KCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~CACG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~CACG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~CACGGCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~GGGACG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~CACGACG ~~~~~~~GCG ~~~~~~~GCG ~CGC~~~~CG ~~~~~~GCCG ~~~~~~~GCG ~~~~~~~GCG ....|....| 185 ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ~CGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT TCGAACAATT ACGAACAATT ACGGACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT Internal transcribed spacer 2 (ITS-2) ....|....| 195 GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCAG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG ....|....| 205 ~GGCGGT~GT ~GGCGGC~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GACGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GACGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GACGGT~GT ~GGCGGC~GT ~GACGGT~GT ~GACGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGAGGC~GT ~GGCGGT~GT CGACGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT CGACGGT~GT ~GGCGGT~GT ~GGCGGG~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT CGACGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GACGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGC~GT ~GGCGGC~GT ~GACGGT~GT ~GGCGGT~GT ....|....| 215 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~CG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~AGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~AGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~CG ~~~~~~~~~~ ~~~~~~~~~~ ....|....| 225 CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGCG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGCG CGCGCTTGTG CGCGCTCGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTCGTG CGCG~~TGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTCGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG AGCGCTTGTG CGCGCTCGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCGTG~~ CGCGCTTGTG CGCGCTTGTG ....|....| 235 TG~~~~~~~~ TG~~~~~~~~ TGTGTG~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TT~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TT~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TT~~~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ TT~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ ~~~~~~~~~~ GG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ ~~~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TGTG~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TGTG~~~~~~ TT~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TGTG~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TT~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ TGTG~~~~~~ ....|....| 245 TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TACG TGGT~~TACG TGGG~~TGCG TGGG~~TACG TGGGGGTGCG TGGGGGTGCG TGGG~~TACG TGGG~~TTCG TGGG~~TACG TGGG~~TACG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~CTCG TGCG~~T~CC TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TACG TGGG~~TTCG TGGG~~TGCG TGGG~~TGCG TGGG~~TTAG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGGG~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TACG TGGATATACG TGGG~~TGCG TGGG~~TACC TGGG~~TGCG TGGG~~TGCG TGGG~~TTCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TACG TGGG~~TGCG TGGG~~TGCG CGGG~~TTCG TGGG~~TGCG TGGG~~TACG TGGG~~TTCG TGGG~~TTCG TGGG~~TACG TGGG~~TGCG 188 ....|....| 255 ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC TCACG~~TCC T~AC~~~TGC ~~~~~~~TCC TCACG~~TCC ~~~~~~~TCC ~~~~~~~TCC TCACG~~TCC ~~~~~~~CCC TCACG~~TCC TCACG~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~CCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~TATCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC TCACG~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC TCACG~~TCC ~~~~~~~TCC ....|....| 265 CTACC~~GCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTGCC~~GCG ATACC~~GCG CTACC~~GCG CTGCC~~GCG CYACC~~GCG CCACC~~GCG CTGCC~~GCG ATGCC~~GCG CTGCC~~GCG CTGCC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CCGCC~~GCG CCACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CCACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTAMY~~GCG CTACC~~GCG CTACC~~GCG CTATC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CCGCC~~GCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG CTACC~~GCG GCACGC~GCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG ....|....| 275 C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCAC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CACCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCC~~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CACCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~GTCCC~ C~~~GTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ CCGCCTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~GTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTACC~ ....|....| 285 G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~CGCGGTCC G~TGCGGTCC G~CGCGGTCC G~CGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~CGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~CGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~CGCGGTCC G~TGCGGTCC G~CGCGGTCC G~CGCGGTCC G~TGCGGTCC G~CGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC ....|....| 295 ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTYAAAA~ ~GTTCAAAT~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAATA ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAA~ ~GTTTAAAA~ ~GTTCAAATA ~GTTTAAAT~ ....|....| 305 ~~ATG~ARA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATGAAGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~ATA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AAAA ~~ATG~AGA~ ~~ATG~AAA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~ATA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AKA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ TTATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ TTATG~AGA~ ~~ATG~AGA~ ....|....| 315 ~CTTA~~~TA ~CATA~~~GA ~CATA~~~GA ~CGTAT~~~A ~CTTG~~~TA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~AA ~CATG~~~AA ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~AA ~CGTA~~~TA ~CGTA~~~TA ~CGTA~~~TA ~CATA~~~GA ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA ~CGTAT~~~A ~CATA~~~~A ~CGTGTTATA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~ATTA~~~TA ~CATA~~~GA ~CGTA~~~TA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~AA ~CACA~~~AA ~CGTAT~~~A ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~SA ~CATA~~~GA ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~GA Appendix 3: Sequence data MW00547 MW01001 MW01011 MW01014 MW01018 MW01019 MW01025 MW01034 MW01039 MW01048 MW01053 MW01059 MW01061 MW01083 MW01092 MW01107 MW01116 MW02001 MW02006 MW02025 MW02033 MW02034 MW98009 MW98029 MW98049 MW98079 MW98097 MW98103 MW98129 MW98136 MW98138 MW98139 MW98154 MW98162 MW98170 MW98172 MW98180 MW98185 MW98189 MW98203 MW98205 MW98212 MW98220 MW98228 MW98235 MW98240 MW98261 MW98264 MW98270 MW98278 MW98285 MW98294 MW98313 MW98315 MW99001 MW99006 MW99009 MW99013 MW99014 MW99018 MW99038 MW99047 MW99057 MW99058 MW99094 MW99095 MW99097 MW99105 ....|....| 165 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~CACG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~CACG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~CACG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~CACG ~~~~~~~~~~ ~~~~~~CACG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ACGGCACACG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ACGGCGCACG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ACGGCGCACG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ....|....| 175 ~~~~~~~GCG ~~~~~~~GCG ~~~CACGACG ~~~~~~~GCG GCGCACGGCG ~~~~~~~GGG ~~~~~~~GCG GCGCACGGCG ~~~~~~~GCG ~~~~~~CACG ~~~~~~~GCG GCGCACGGCG ~~~~~~AACG ~~~~~CGACG GCGCACGGCG ~~~~~~~GCG GCGCACGGCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~~TG ~~~~~~CACG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG GCGCAGGGCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG GCGCACGGCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~CACGGCG GCGCACGGCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~CGCG ~~~NNNNNNN ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~CGCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~AGCG ~~~~~~~GGG ~~~~~~AACG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~CACG ~~~~~~~GCG ....|....| 185 ACGAACAATT ACGAACAATT TCGAACAATT ACGAACAATT TCGAACAATT ACGAACAATT ACGAACAATT TCGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT TCGAACAATT ACGAACAATT ACGAACAATT TCGAACAATT ACGAACAATT TCGAACAATT ACGGACAATT ACGAACAATT TCGAACAATT ACGAACAATT ACGAACAATT ACGGACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACKAACGATT TCGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT TCGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT GCGAACAATT TCGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT GCGAACAACT NNNNNNNNNN ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGGACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT Internal transcribed spacer 2 (ITS-2) ....|....| 195 GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACG~TTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCT GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACTGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG NACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACTGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG ....|....| 205 ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GACGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGC~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGC~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GAC~~~~GT ~GGCGGC~GT ~GGCGGC~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGC~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGC~GT ~GGCGGT~GT ....|....| 215 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~AGAGCG ~~~~~~~~~~ ~~~~AGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~AGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~AGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~AGAGCG ~~~~~~~~~~ ~~~~AGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~G ~~~~~~~~~~ ~~~~~~~~CG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~CGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~CGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~CG ~~~~CGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~AGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ....|....| 225 CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCGTGTG CGCGCGTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCTTGTG CACG~~~~~~ CGCGCGTGTG CGCGCGTG~~ CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTCGTG CGCGCTTGTG CGCGCTTGTG CGCGCGCGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTCGTG CGCGCTTGTG CGCGCCTGTG CGCGCTTGTG CGCG~~TGCG CGCGCGTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG YSCGCTTGTG CGCGCGTGTG CGMGCGTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGCG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTCGTG ....|....| 235 TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TT~~~~~~~~ TT~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ ~~~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TT~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TGTGCG~~~~ TG~~~~~~~~ TGTGCG~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTGTG~~~~ TGTG~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TGTG~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ ~~~~~~~~~~ TGTA~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TGTGTG~~~~ TGTG~~~~~~ ~~~~~~~~~~ TGTGTG~~~~ TG~~~~~~~~ TG~~~~~~~~ TT~~~~~~~~ ~~~~~~~~~~ TG~~~~~~~~ TGC~~~~~~~ TGTGTG~~~~ TG~~~~~~~~ TGTGTGTGTG TG~~~~~~~~ ~~~~~~~~~~ TG~~~~~~~~ ....|....| 245 TGGG~~TGCG TGGG~~TGCG CGGG~~TTCG TGGG~~TGCG CGGG~~TTCG TGGGTGTACG TGGG~~TACG CGGG~~TTCG TGGG~~TGCG TGGG~~TTCG TGGG~~TGCG CGGG~~TTCG TGGG~~TTCG TGGATATGCG CGGG~~TTCG TGGG~~TGCG CGGG~~TTCG TGGT~~TACG TGGG~~TACG TGGG~~CGCG TGGG~~TTCG TGGG~~TTCG TGGG~~TGCG TGGG~~TACG TGGG~~TGCG TGGGG~TGCG TGGG~~TGCG TGGG~~TGCG CGGG~~TTCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG CGGG~~TTCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TTCG CGGG~~TTCG ~~~~~~TA~~ TGGG~~TGCG TGGG~~TGCG ~~~~~~TA~~ TGGG~~TTCG CGGG~~TTCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TTCG TGGG~~TGCG TGGG~~TGCG TGGG~~TACG TGGGTGTACG GGGG~~TTCG ~~~~~~TA~~ AGGT~~TACG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TTCG TGGG~~TGCG 189 ....|....| 255 ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC T~ACGTATCC TCACK~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~CCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~CCC ~~~~~TATCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TAC ~~~~~~~TCC ~~~~~~~CGC ~~~~~~~CCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~CCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~~~~ ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~~~~ ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~CCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~TATCC ~~~~~~~TCC ~~~~~~~~~~ ~~~~~~~TAC ~~~~~~~TGC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~CCC ~~~~~~~TCC ....|....| 265 CTACC~~GCG CTACC~~GCG GCACGC~GCG CTACC~~GCG GCACGC~GCG CTACC~~GCG CTGCC~~KCG GCACGC~GCG CTACC~~GCG ATGCC~~GCG CTACC~~GCG GCACGC~GCG ANGCC~~GCG CTACC~~GCG GCACGC~GCG CTACC~~GCG GCACGC~GCG CTGCC~~GCG CTGCC~~GCG G~A~~GCGCG ATGCC~~GCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG G~~~~~~~~~ CTGCC~~GCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG CTACC~~GCG G~~~~~~~~~ CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTGCCGCGCG GCACGC~GCG ~~~~~~~~~~ CTACC~~GCG CTACC~~GCG ~~~~~~~~~~ CTGCC~~GCG GCACGC~GCG CTACC~~GCG CTACC~~GCG CTGCC~CGCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG CTACC~~GCG CTACCGCGCG CTACC~~GCG CTCCC~~GCG ~~~~~~~~~~ CTGCC~~GCG CTGCC~~KCG CTACC~~GCG CTACC~~GCG CTGCC~~GCG CTGCC~~GCG CTACC~~GCG ....|....| 275 C~~~CTNCC~ C~~~CTCCC~ CCGCCTCCC~ C~~~CTCCC~ CCGCCTCCC~ C~~~CTCCC~ C~~~CTCCC~ CCGCCTCCC~ C~~~CTCCC~ C~~~CTCAC~ C~~~CTCCC~ CCGCCTCCC~ C~~~CTCAC~ C~~~CTCCC~ CCGCCTCCC~ C~~~CTCCC~ CCGCCTCCC~ C~~~CTCCC~ C~~~CTCCC~ CCTCGTCTC~ C~~~CTCAC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ ~~~~~~~~~~ C~~~CTCCCC C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ ~~~~~~~~~~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ CCGCCTCCC~ ~~~~~~CCC~ C~~~CTCCC~ C~~~CTCCC~ ~~~~~~CCC~ C~~~CTCCC~ CCGCCTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCGC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTACC~ C~~~CTCCC~ C~~~TTCCC~ ~~~~~~CCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCGC~ C~~~CTCCC~ ....|....| 285 G~CGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~AACGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC ~~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC ~~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCA G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGKCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC ....|....| 295 ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTCAAAT~ ~GTTCAAAT~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTTAAAA~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTTAAAA~ ~GTTCAAAT~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTCAAAA~ ~GTTTAAAA~ ~GTTTAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAA~ CGTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAA~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ CGTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAA~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAATA ~GTTCAAAT~ ~GTTTAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTKTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTTAAAA~ ~GTTTAAAT~ ....|....| 305 ~~ATG~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATT~~~~~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AAA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGAA ~~ATG~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATR~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ TTGTG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~ANA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ....|....| 315 ~CATA~~~GA ~CATA~~~AA ~CATA~~~AA ~CATA~~~GA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~TA ~CACA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~C~~~~~~AA ~CATA~~~TA ~CATA~~~AA ~CTTA~~~TA ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATTA~~GA ~CATA~~~GA ~CATATA~TA ~CATA~~~KA ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA ~CTTA~~~TA ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~AA ~CGTA~~~TG CTTTG~~~TA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~GA ~CGTA~~~TA ~CACATA~KA ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~SA ~CATA~~~GA ~CATA~~~GA ~CTTG~~~TA ~CATA~~~AA ~CATA~~~GA Appendix 3: Sequence data MW99134 MW99135 MW99163 MW99164 MW99174 MW99196 MW99203 MW99226 MW99240 MW99247 MW99274 MW99276 MW99286 MW99292 MW99301 MW99303 MW99309 MW99319 MW99341 MW99353 MW99372 MW99374 MW99382 MW99393 MW99406 MW99407 MW99408 MW99413 MW99448 MW99465 MW99471 MW99479 MW99501 MW99508 MW99514 MW99536 MW99537 MW99546 MW99550 MW99552 MW99559 MW99565 MW99591 MW99605 MW99608 MW99612 MW99613 OK96022 WE00002 WE02431 WE02451 WE02454 WE02491 WE02531 WE02534 WE02535 WE02591 WE02614 WE02621 WE02661 WE02662 WE02671 WE02674 WE02677 WE85001 WE94001 ....|....| 165 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ACGGCGCACG ~~~~~~CACG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~CACG ~~~~~~~~~~ ~~~~~~CACG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ....|....| 175 ~~~~~~GACG ~~~~~~~GCG ~~~~~~CACG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~CACG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG GCGCACAGCG GCGCACGGCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~CACGACG GCGCACGGCG ~~~~~~~GCG GCGCACGGCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ~~~~~~~GCG ....|....| 185 ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGGACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT TYTAACAATT TCGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT TCGAACAATT TCGAACAATT ACGAACAATT TCGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAAYT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT ACGAACAATT Internal transcribed spacer 2 (ITS-2) ....|....| 195 GACGGTTCCG GACGGTTCCG GACGGCTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGCTCCG GACKGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG RACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG GACGGTTCCG ....|....| 205 ~GRCGGT~GC ~GGCGGT~TG ~GGCGGTTGT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~TG ~GGCGGG~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGG~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GCCRGT~GT ~GCCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGG~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ~GGCGGT~GT ....|....| 215 ~~~~~~AGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ AYAGMGCGCG ~~~~AGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~AGAGCG ~~~~AGAGCG ~~~~~~~~~~ ~~~~AGAGCG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~G ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ....|....| 225 CGCGCGCGNN CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTCGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCGCGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCGTGTG CGCGCGTGTG CGCGCTTGTG CGCGCGTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG TGCGCACGTG CGCGCTCGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG CGCGCTTGTG ....|....| 235 ~~~~~~~~~~ TG~~~~~~~~ ~~~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTGTG~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ ~~~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTGTG~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTGTG~~~~ TGTGTG~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TGTGTG~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TGTG~~~~~~ TGTG~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTGTGTG~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TGTG~~~~~~ TG~~~~~~~~ TG~~~~~~~~ TG~~~~~~~~ ....|....| 245 NNNN~~NNNN TGGG~~TGCG TGGG~~TTCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGGG~TGCG TGGG~~TGCG TGGG~~TACR TGGG~~TTCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGGG~TGCG TGGGG~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG CGGG~~TTCG CGGG~~TTCG ~~~~~~TA~~ TGGG~~TACG ~~~~~~TA~~ TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG CGGG~~TTCG CGGG~~TTCG TGGG~~TACG CGGG~~TTCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~CGCG TGGG~~TGCG TGGGG~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGGG~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG TGGG~~TGCG 190 ....|....| 255 ~~~~~~~NNN ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~YCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~~~~ ~~~~~~~TCC ~~~~~~~~~~ ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ~~~~~~~TCC ....|....| 265 NNNNN~~NNN CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CCACC~~GCG CTGCC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTGCC~~GSC CTGYC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTGCC~~GCG CTGCC~~GCG CTACC~~GCG CCACC~~GCG CTACC~~GCG YTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG GCACGC~GCG GCACGC~GCG ~~~~~~~~~~ CTACC~~GCG ~~~~~~~~~~ CTGCC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG GCACGCGGCG GCACGC~GCG CTACC~~GCG GCACGC~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTGCC~~GCG CTACC~~GCG CCACC~~GCG YTACC~~GCG CTACC~~GCG CTACC~~GCG YTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG CTACC~~GCG ....|....| 275 N~~~NNNNN~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ CCGCCTCCC~ CCGCCTCCC~ ~~~~~~CCC~ C~~~CTCCC~ ~~~~~~CCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ CCGCCTCCC~ CCGCCTCCC~ C~~~CTCCC~ CCGCCTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~YTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ C~~~CTCCC~ ....|....| 285 N~NNNNNNNN G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC GGTGCGGTCC G~TGCGGTCC GGTGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC G~TGCGGTCC ....|....| 295 ~NNNNNNNN~ ~GTTTAAAT~ ~GTTTAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTGAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTCAAAA~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTCAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ~GTTTAAAT~ ....|....| 305 ~~NNN~NNN~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~ANAC ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~AT~~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ~~ATG~AGA~ ....|....| 315 ~NNNN~~~NN ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CGTA~~~TA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CTTA~~~TA ~CTTATAATA ~CATA~~~AA ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA TCATAA~~TA ~CATA~~~GA ~CATA~~~GA ~CGTA~~~TA ~CATA~~~GA ~ATTA~~~TA ~ATTA~~~TA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CGTA~~~TA ~CATA~~~GA ~CGTA~~~TA ~CATA~~~AA ~CATA~~~AA ~CATA~~~AA ~CATA~~~GA ~CATA~~~AA ~CTTG~~~TA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~AA ~CATA~~~AA ~CATA~~~GA ~CATA~~~AA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CGTA~~~TA ~CATA~~~GA ~CATA~~~GA ~CTTA~~~TA ~ATTA~~~TA ~CAYA~~~GA ~CGTA~~~TA ~CATA~~~GA ~ATTA~~~TA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA ~CATA~~~GA Appendix 3: Sequence data AD98001 AD98009 AD98012 AD98018 AD98024 AD98036 DS00001 DS01001 JC00029 JC00039 JC00040 JC00042 JC00043 JC00045 JC00051 JC00055 JC00061 JC00063 JC01014 JM00001 MW00015 MW00024 MW00032 MW00051 MW00058 MW00064 MW00072 MW00076 MW00101 MW00102 MW00110 MW00116 MW00127 MW00129 MW00151 MW00176 MW00177 MW00178 MW00179 MW00185 MW00189 MW00226 MW00231 MW00232 MW00234 MW00262 MW00267 MW00269 MW00290 MW00302 MW00316 MW00326 MW00328 MW00330 MW00347 MW00348 MW00393 MW00409 MW00412 MW00444 MW00452 MW00469 MW00498 MW00504 MW00517 MW00525 MW00530 MW00539 ....|....| 325 T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~G~~~~~~~ T~G~~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GTTATTAA C~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~T~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~A~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~T~~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GTT~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~G~~~~~~~ T~G~~~~~~~ T~GT~~~~~~ ....|....| 335 ~AATA~~~TA ~AATA~~~TG ~AATA~~~TA ~AATATTATA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TG TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TG TAATA~~~TA TAATA~~~TG ~AATA~~~TA ~AATATTATA ~AATATTATA TAATA~~~TA ~AATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATATTATA AAANG~~~TA ~AATA~~~TG ~AATA~~~TG ~AATA~~~TG ~AATA~~~TA ~AATA~~~TG TAATA~~~TG GATTA~~~TA TAATA~~~TA TAATA~~~TA ~AATA~~~TA TAATA~~~TG TAATATTATA ~AATA~~~TG ~AATA~~~TA ~AATA~~~TA ~AATAT~ATA TAATA~~~TA TTATTT~~TA ~RATATTATA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TA TAATA~~~TA ~AATA~~~TA CAATA~~~TA TAATA~~~TA TAATA~~~TA ~AATA~~~TA ....|....| 345 A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG ATG~T~AACG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG AC~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG Internal transcribed spacer 2 (ITS-2) ....|....| 355 ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TCA~~CGTG ~TTG~~CGTG ~TTA~~AGTG ~TTG~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG CTTC~ACGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTN~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~CCGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ....|....| 365 TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TYGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGC~~~~ TCGTGC~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGC~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGC~~~~ TCGTGC~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ ....|....| 375 ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTCTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ....|....| 385 ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TGC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~GTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TACCAC ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ....|....| 395 GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTGGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGT GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC ....|....| 405 TCGCGTCGGC TCGCGACGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTMGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGAC TCGCGTYGGC TCGCGTCGGC TCGCGACGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGACGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC 191 ....|....| 415 GACGTGCGCC GACGCGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACRTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GRCGTGCGCC GACGTGCGCC GACGCGCGCC GACGCGCGCC GACGTGCGCC GACGCGCGCC GACGCGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGTGCGCC SACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC ....|....| 425 GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCAGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC ....|....| 435 CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCCCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG ....|....| 445 TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAG TGAAGGTGAG TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTAAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAG TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA NGAAGGTNNN TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAG TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTKAA TGAAGGTGAA TGAACGTGAA TGAAGGTGAA TGAAGGTGAA ....|....| 455 AAG~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAG~~~~~~~ AAAA~~~~~~ AAA~~~~~~~ AGA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ NNN~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AGA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ ....|....| 465 ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTAG~~~GA ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~NN ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~NNNN~~~NN ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~NN ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG TTTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~CTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ....|....| 475 AGAGCGATTA AGAGCGATTA AGAGCGATTA MCAGNNNNNN AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGTGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA NNNNNNNNNN AGAGCGATTA ASAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA NNNNNNNNNN AGAGCGATTA AGAGCGATTA NNNNNNNNNN AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGARCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA Appendix 3: Sequence data MW00547 MW01001 MW01011 MW01014 MW01018 MW01019 MW01025 MW01034 MW01039 MW01048 MW01053 MW01059 MW01061 MW01083 MW01092 MW01107 MW01116 MW02001 MW02006 MW02025 MW02033 MW02034 MW98009 MW98029 MW98049 MW98079 MW98097 MW98103 MW98129 MW98136 MW98138 MW98139 MW98154 MW98162 MW98170 MW98172 MW98180 MW98185 MW98189 MW98203 MW98205 MW98212 MW98220 MW98228 MW98235 MW98240 MW98261 MW98264 MW98270 MW98278 MW98285 MW98294 MW98313 MW98315 MW99001 MW99006 MW99009 MW99013 MW99014 MW99018 MW99038 MW99047 MW99057 MW99058 MW99094 MW99095 MW99097 MW99105 ....|....| 325 T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~A~~~~~~~ T~G~~~~~~~ T~G~~~~~~~ T~A~~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~A~~~~~~~ T~G~~~~~~~ T~G~~~~~~~ T~A~~~~~~~ T~GT~~~~~~ T~A~~~~~~~ T~G~~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~G~~~~~~T T~GT~~~~~~ T~T~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~A~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ ATGT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~A~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ TTGT~~~~~~ T~A~~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~T~~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GTT~~~~~ TTG~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~TG T~G~~~~~~~ T~G~~~~TAA T~G~~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ TTG~~~~~~~ T~GT~~~~~~ ....|....| 335 ~AATA~~~TA TAATA~~~TG TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TG TAATA~~~TA TAATA~~~TG TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TG TAATA~~~TA TAATA~~~TA TAATA~~~TA TA~~~~~~~~ TAATA~~~TA TAATA~~~TA ~AATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TA ~AATA~~~TG ~AATA~~~TA TAATA~~~TG ~AATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TG TAATA~~~TG TGATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA ~AATA~~~TG ~AATG~~~TA ~AATA~~~TA ~AATA~~~TG TAATA~~~TA ~AATA~~~TG ~AATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TG ~AATA~~~TA ~AATA~~~TA TAATA~~~TA TAATA~~~TA ....|....| 345 A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG ~~~~~~~~~~ A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG G~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~CGT A~~~~~~~CK A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG Internal transcribed spacer 2 (ITS-2) ....|....| 355 ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~AGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~~~~~~~~~~ ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTACACGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ....|....| 365 TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGC~~~~ TTGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ ~~~~~T~~~~ TTGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ YC~TGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ ....|....| 375 ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTGTG~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTGTG~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTGTG~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTGTG~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ....|....| 385 ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~~~C~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~GTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TRC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TACCGC ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TGC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ....|....| 395 GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC ~CGAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCSCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGT GGTAGCGCGC GGCAGCGCGC GGTAGCGCGT GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGT GGTAGCGCGC GGTAGCGCGC GGTAGCGCGT GGTAGCGCGC GGTAGSKCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC ....|....| 405 TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTACGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCTCGTTAGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGACGGC TCGCGTSGGA TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGM TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCNTTNGT TCGCGACGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC 192 ....|....| 415 GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGCGCGCC GACGCGCGCC GACGCGCGCC GACGCGCGCC SACGCGCGCC GACGTGCGCC GACGCGCGCC GACGTGCGCC GACGCGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGTGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGCRCGCC GACGCGCGCC GACGCGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC ....|....| 425 GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCAC GCT~CCCCAC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC CCG~CC~~GC GCT~CCCCAC GCT~CCCCAC GCT~CCCCGC GYT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCTCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCAC GCT~CCCAGC GCT~CCCCGC GCT~CCCCGC GCT~CCCAGC GCT~CCCCAC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCAGC GCT~CCCCGC GCT~CCCYGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC ....|....| 435 CGACGCTCCG CGACGCTC~~ CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CKACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG SGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG ....|....| 445 TGAAGGTGAA ~~~AGGTNNN TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGGA NGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTAAA TGAAGGTGAA TGAAGGTGAA TGAAGGTAAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGTTGTG TGAAGGTAAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGARGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGGA TGAAGGTGAA TGAAGGTGAA TGAAGGTAAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTAAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTAAA TGAAGGTGAA TGAAGGTGAA TGAAGGTAAA TGAAGGTGAA TGAAGGTAAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTAAA TGAAGGTGAA ....|....| 455 AAA~~~~~~~ NNN~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ ACTATAACAC AAA~~~~~~~ AAAC~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAAAAA~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AGA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ ....|....| 465 ~TTTG~~~CG ~NNNN~~~NN TTTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TNNN~~~NN ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG TTGTG~~~CG ~TTTG~~~CG ~TTTGAGACG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG TTTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ....|....| 475 AGAGCGATTA NNNNNNNNNN AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA NNNNNNNNNN AGAGCGATTA AGAGCGATTA AGAGTGATTA AGAGCGATTA AGAGCGATTA AGAGTGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGTGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA WGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGTGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGTGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGTGATTA AGAGCGATTA Appendix 3: Sequence data MW99134 MW99135 MW99163 MW99164 MW99174 MW99196 MW99203 MW99226 MW99240 MW99247 MW99274 MW99276 MW99286 MW99292 MW99301 MW99303 MW99309 MW99319 MW99341 MW99353 MW99372 MW99374 MW99382 MW99393 MW99406 MW99407 MW99408 MW99413 MW99448 MW99465 MW99471 MW99479 MW99501 MW99508 MW99514 MW99536 MW99537 MW99546 MW99550 MW99552 MW99559 MW99565 MW99591 MW99605 MW99608 MW99612 MW99613 OK96022 WE00002 WE02431 WE02451 WE02454 WE02491 WE02531 WE02534 WE02535 WE02591 WE02614 WE02621 WE02661 WE02662 WE02671 WE02674 WE02677 WE85001 WE94001 ....|....| 325 T~T~~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~GTT~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~A~~~~~~~ T~A~~~~~~~ T~G~~~~~~~ T~GT~~~~~~ T~G~~~~~~~ TTGT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~G~~~~~~~ T~A~~~~~~~ T~GT~~~~~~ T~A~~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ T~GT~~~~~~ ....|....| 335 TAATA~~~TA ~AATA~~~TA TAATA~~~TA TAATA~~~TG ~AATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TG TAATA~~~TG TAATA~~~TG ~AATA~~~T~ ~AATA~~~TA TAATA~~~TA TAATA~~~TA ~AATA~~~TA TAATA~~~TG ~AATA~TATA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TG ~AATA~~~TA TAATA~~~TA ~AATA~~~TG ~AATATTATA ~AATA~~~TA ~AATATTATA TAATA~~~TA TAATA~~~TA TAATAATATA ~AATA~~~TA TAATAATATA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TG TAATA~~~TG TAATA~~~TA TAATA~~~TA ~AATA~~~TA TAATA~~~TA ~AATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA TAATA~~~TA TAATA~~~TA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TA ~GATATTATA ~AATA~~~TA ~AATA~~~TA ~AATA~~~TG TAATA~~~TA ~AATA~~~TA ~AATA~~~TA ....|....| 345 A~~~~~~~CG A~~~~~~~CG AMCKTWAACG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG ~~~~~~~~~G A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CN A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG A~~~~~~~CG Internal transcribed spacer 2 (ITS-2) ....|....| 355 ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTN~~CNTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TYA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTA~~CGTG ~TTR~~CGTG ~TTA~~CGTG ~TTA~~CGTG ....|....| 365 TTGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGTCGTG TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGC~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCTGGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TTGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGC~~~~ TCGTGT~~~~ TCGTGT~~~~ TCGTGT~~~~ ....|....| 375 ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ TGGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ~GGTGTG~~~ ....|....| 385 ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TACCAC ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TACCGC ~~~~TACCGC ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TGC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ TGTGTAC~~~ TGTGTAC~~~ ~~~~TAC~~~ TGTGTAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TGC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TACCAC ~~~~TACCAC ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TACCAC ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ~~~~TAC~~~ ....|....| 395 GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTACCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGT GGTAGCGCGC GGTAGCGCGT GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGGAACNCGC GGWAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAACCCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC GGTAGCGCGC ....|....| 405 TCGMGTCGAC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTMGGN TCGCGTCGGC TCGCGACGGC TCGCGTCKSC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCCTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGACGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGM TCGCGTCGGC TCGCGTNGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC TCGCGTCGGC 193 ....|....| 415 RACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGCGCGCC KACGTGCRCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGTGCGCC WACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGCGCGCC GACGCGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC AACGTGCNCC GACGTGCGCC GACGCGCGCC GACGTGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC GACGCGCGCC GACGTGCGCC GACGTGCGCC GACGTGCGCC ....|....| 425 GCT~CCCCAC GCT~CCCCGC GCCACCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCCACCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCTC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCAC GCT~CCCAGC GCT~CCCCGC GCT~CCCAGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCAC GCT~CCCCAC GCT~CCCCGC GCT~CCCCAC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GYT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GNT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC GCT~CCCCGC ....|....| 435 CGACGMTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG MGACGCTCCG CGACGCTCCG CGACGCTCCG MGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCNN CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACKCTCCG CGACGCTCCG CGACGYTCCG CGACGCTCCG CGACGCTCCG CGACGCTYCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG CGACGCTCCG ....|....| 445 TGAAGGTAAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA NNNNNNNGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAG TGAAGGTGAG TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAG TGAAGGTGAA TGAAGGTGAA T~AAGGTAAA TGAAGGTGGA TGAAGGTGAA TGAAGGTGAA T~AAGGNKAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGGA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA TGAAGGTGAA ....|....| 455 AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AGA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAG~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAT~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ AAA~~~~~~~ ....|....| 465 ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TKTA~~~CG ~TTTA~~~CG ~TTTG~~~CG ~NNNN~~~NN ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTA~~~CG ~TTTA~~~CG TTTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CR ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ~TTTG~~~CG ....|....| 475 AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCKATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA NNNNNNNNNN AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCNATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCRATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA AGAGCGATTA Appendix 3: Sequence data AD98001 AD98009 AD98012 AD98018 AD98024 AD98036 DS00001 DS01001 JC00029 JC00039 JC00040 JC00042 JC00043 JC00045 JC00051 JC00055 JC00061 JC00063 JC01014 JM00001 MW00015 MW00024 MW00032 MW00051 MW00058 MW00064 MW00072 MW00076 MW00101 MW00102 MW00110 MW00116 MW00127 MW00129 MW00151 MW00176 MW00177 MW00178 MW00179 MW00185 MW00189 MW00226 MW00231 MW00232 MW00234 MW00262 MW00267 MW00269 MW00290 MW00302 MW00316 MW00326 MW00328 MW00330 MW00347 MW00348 MW00393 MW00409 MW00412 MW00444 MW00452 MW00469 MW00498 MW00504 MW00517 MW00525 MW00530 MW00539 ....|....| 485 TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ NNNNNNNN~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGTG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ NNNNNNNN~~ TCGGTGAG~~ TCGGCCAR~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ NNNNNNNN~~ TCGGCGAG~~ TCGGCGAG~~ NNNNNNNN~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~G TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ ....|....| 495 CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC NNNNNNNNNN CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGN CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGAGC CGGGGCGTGC CGGGGCGTGC NNNNNNNNNN CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC NNNNNNNNNN CGGGGCGAGC CGGGGCGTGC NNNNNNNNNN CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGNNNNN CGGGGCGTGC AGGGGCGTGC CGGGGCGTGC CGGGGCGTGC ....|....| 505 ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ NNNN~~NN~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ NNNN~~NN~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ GCGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ NNNN~~NN~~ ACGCC~AG~~ ACSC~~KC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ NNNN~~NN~~ GCGC~~AC~~ ACGC~~GC~~ NNNN~~NN~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ NNNN~~NN~~ ACGC~~AC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ Internal transcribed spacer 2 (ITS-2) ....|....| 515 ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~NN~NNNN~N ~GT~CKAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CKAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CRAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CKAA~C ~GT~CGAA~C ~GT~CGAA~C ~NN~NNNN~N ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~KT~CTAA~C ~GT~CGAA~C ~GT~CGAA~C ~NN~NNNN~N ~GT~CGAA~C ~GT~HSWA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~NN~NNNN~N ~GT~CGAA~C ~GT~CGAA~C ~NN~NNNN~N ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~NN~NGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ....|....| 525 T~GTGT~CGC T~GTGT~CGT T~GTGT~CGT N~NNNN~NNN T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT N~NNNN~NNN T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GGGT~CGT N~NNNN~NNN TTGCGT~CGT T~GTGT~YGM T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT N~NNNN~NNN T~GTGT~CGT T~GTGT~CGT N~NNNN~NNN T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT Y~KTKT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT TTGTGT~CGT T~GTGT~CGT T~GTGT~TGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT ....|....| 535 ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~NNNNNNNN~ ~CKGCGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CKGCGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~NNNNNNNN~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CAACKCKC~ ~CGACGCGC~ ~CGACGCGC~ ~NNNNNNNN~ ~CGACGCGC~ ~CGACSCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CRACGCGC~ ~CGACGGGC~ ~CKACGCGC~ ~CGACGCGC~ ~CKACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~NNNNNNNN~ ~CGACGCGC~ ~CGACGCGC~ ~NNNNNNNN~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGCG ~CGACGCGC~ ~YGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ....|....| 545 ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~NNNNNNNN~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~GTGATGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~NNNNNNNN~ ~AGAACGCG~ ~AGCACGCG~ ~AGCACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AAGGCGCG~ ~AGAACGCG~ ~AGAACGCG~ ~NNNNNNNN~ ~AGAGTGCG~ ~ARAACGCG~ ~AGRACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGGACGCG~ ~AGGACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGCACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~NNNNNNNN~ ~AGAACGCG~ ~AGAACGCG~ ~NNNNNNNN~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ CAGAATGCG~ ~AGAACGCG~ ~ARAACGCG~ ~AGAATGCG~ ~AGAATGCG~ ~AGAACGCG~ ~AGAACGCG~ ....|....| 555 CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA NNNNN~~~NN CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~TGTA CTGCG~~~TA CTGCG~~~TA CTGCG~TGTA CTGCG~~~TA CTGCG~~~TA CTGCGGTGTA CTGCG~~~TA CTGCG~TGTA CTGCG~TGTA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA NNNNN~~~NN CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~TGTA CTGCG~~~TA CTGCG~~~TA NNNNN~~~NN CTGCG~CGTA CTGSG~~K~A CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~CA CTGCG~~~TA CTGCG~~~TG CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA NNNNN~~AAA CTGCG~TGTA CTGCG~~~TA NNNNN~~~NN CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~TGTA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGGGK~~~A CTGCG~~~TA CTGCG~~~TA CTGCG~TGTA CTGCG~~~TA ....|....| 565 CACGTTTTTT CACGTTTTTT CGCGTTTCTT NNNNTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTCTTTT CACTCTTTT~ CACGTTTTTT CACGTTTTTT CACTCTTTT~ CACGTTTTTT CACGTTTTTT CACTCTTTT~ ~~CGTT~~~~ CACTCTTTTT CACTCTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTT~ ~~NNNN~~~~ CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTT~ CACGTTTTTT CACGTTTTTT NNNNNNNNNN C~~GTTT~~~ CACGTTTTTT CACGTTTCTT CACGTTTTTT CACGTTTTTT CACGTTTCTT CACGTTTCTT CACGTTTTTT CACGTTTTT~ CACGTTTTTT CA~~~~~~~~ CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CMAGTTTTTT CRCGTTTTT~ CACGTTTTTT NNNNNNNNNN CACGTTTTTT CACGTTTTTT CACGTTTCTT CACGTTTTTT CACGTTTTTT CACGTTTCTT CACGTTTCTT CACTCTTTTT CACGTTTTTT CACGTTTTTT CTCGTT~~~~ CACGTTTTTT CACGTTTTTT ~~CGTTTTT~ ~~CGTTTTTT CACTCTTTTT CACGTTTTTT 194 ....|....| 575 ~~~~~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ T~~~~~~~~~ GT~~~~~~~~ ~~~~~~~~~~ TTT~~~~~~~ T~~~~~~~~~ CCT~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ CCT~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ CCT~~~~~~~ ~~~~~~~~~~ CCT~~~~~~~ CCT~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ C~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ T~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ GCGT~TTAAC ~~~~~~~~~~ CTTTT~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ CTTTT~~~~~ CTTTT~~~~~ CA~~~~~~~~ C~~~~~~~~~ TT~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TTTTTTTTTT ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ T~~~~~~~~~ CCT~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~C ~~~~~~~~~~ AATG~~~AA~ ~~~~~~~~~~ ~~~~~~~~~~ CCT~~~~~~~ ~~~~~~~~~~ ....|....| 585 ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~GACG ~~~~~~GACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~~~CG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~TGTACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~NNNN ~~~TGTTT~A ~~~~~~AACG ~~~~~~AAMG ~~~~~~AACG ~~~~~~AACG ~~~~~~AAAG ~~~~~~AAAG ~~~~~TAACG ~~~~~~AACG ~~~~~~AAMG ~~~~~~~~~~ ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AAMG ~~~~~~AACG G~CAAAAAGG ~~~~~~NNNN ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG GTTCGTTGCG ~~~~~~AACG ~~~~~~AAAA ~~~~~~AAC~ ~~~~~~~~~~ ~~~~~~AACG ~~~~~~AACG ....|....| 595 AAT~~~~AAA AA~~~~~AAA AA~~~~AGAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~GAAA AT~~~~~AAA AA~~~~~AAA AA~~~~GAAA AA~~~~~AAA AA~~~~~AAA AA~~~~GAAA AA~~~CGAAA AA~~~~GAAA AA~~~~GAAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AGAGT~~~~~ AA~~~~AAGA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~CG~CG AA~~~~AAAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~AAAA AA~~~~~AAA AA~~~~~AAA AA~~~~AAAA ~~~~~AAAAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~AGAA AA~~~~~AAA AA~~~AAAAA AA~~~~~AAA AA~~~AAAGA NN~~~~~NNN GA~~~~~AAA AA~~~~AAAA AA~~~~AAAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~GAAA AA~~~~~AAA AA~~~~~AAA AA~~~CGCCG AA~~~~~AAA AA~AAAGAAA AA~~~~~AAA ~~~~~~~~~A AA~~~~GAAA GA~~~~~AAA ....|....| 605 A~~NN~~~NN A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC G~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~TC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CG~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~CACC N~~NN~~~NN A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ~~~CAACACC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ....|....| 615 NNNN~~~~NN ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC GCCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC GCCG~~~~TC ACCG~~~~TC ACCGTCTGTC ACCG~~~~TC ACCG~~~~TC ACCGTCTGTC ACCGTCTGTC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC NNNN~~~~~C ACCG~~~~TC ACCG~~~~TC ACAGTCTGTC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ....|....| 625 N~NNNNNNNN G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT A~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACGCT G~GAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACGCT G~AAAACACT C~GAAACACT G~AAAACACT G~AAAACACT C~GAAACACT C~GAAACACT G~GAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT K~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT S~AAAA~~~~ G~AAAACACT G~AAAACACT C~GAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT GAAAAACACA G~AAAACACT G~AAAACACT ....|....| 635 N~~~NNNN~~ G~~~TTAT~~ G~~~TTAT~A G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTATTA G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~TGTTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ GTTGTATT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ ~~~~~~~~~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ Appendix 3: Sequence data MW00547 MW01001 MW01011 MW01014 MW01018 MW01019 MW01025 MW01034 MW01039 MW01048 MW01053 MW01059 MW01061 MW01083 MW01092 MW01107 MW01116 MW02001 MW02006 MW02025 MW02033 MW02034 MW98009 MW98029 MW98049 MW98079 MW98097 MW98103 MW98129 MW98136 MW98138 MW98139 MW98154 MW98162 MW98170 MW98172 MW98180 MW98185 MW98189 MW98203 MW98205 MW98212 MW98220 MW98228 MW98235 MW98240 MW98261 MW98264 MW98270 MW98278 MW98285 MW98294 MW98313 MW98315 MW99001 MW99006 MW99009 MW99013 MW99014 MW99018 MW99038 MW99047 MW99057 MW99058 MW99094 MW99095 MW99097 MW99105 ....|....| 485 TCGGCGAG~~ NNNNNNNN~~ TCGGTGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ NNNNNNNN~~ TCGGTGAG~~ TCGGCGAG~~ TCGGTGTG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGTGTG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGT~TA~~ TCGGTGTG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCRAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGMGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGTG~~ TCGGTGAG~~ TCGGMGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGTG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TMKGYCAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGTG~~ TCGGCGAG~~ ....|....| 495 CGGGGCGTGC NNNNNNNNNN CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC NNNNNNNNNN CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC AGGG~CGGGY CGGGGCGTGC AGGGGCGTGC CGGGGCGTGC CGGGGCGAGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCG~GC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC ....|....| 505 ACGC~~GC~~ NNNN~~NN~~ GCGC~~AC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ NNNN~~NN~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~KC~~ ACGC~~AC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~AC~~ ACGC~~GC~~ GTGC~~AC~~ ACGC~~AC~~ ACGC~~AC~~ ACGC~~GC~~ GCGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGCGCAC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ GCGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~NN~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ Internal transcribed spacer 2 (ITS-2) ....|....| 515 ~GT~CGAA~C ~NN~NNNN~N ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~NN~NNNN~N ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~NN~NNNN~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~~T~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~MAAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~A ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CKAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~ANAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CKAA~C ~GT~CGAA~C ~GT~CYAA~C ~GT~CGAA~C ~NN~NNNN~N ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ....|....| 525 T~GTGT~CGT N~NNNN~NNN TTGTGT~CGT T~GTGT~CGT TTGTGT~CGT T~GTGT~CGT N~NNNN~NNN TTGTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CKT TTGTGT~CGT T~GTGT~CGT T~GTGT~CGT TTGTGT~CGT T~GTGT~CGT TTGTGT~CGT T~GTGT~CGT T~GTGT~CGT TT~TGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT TTGTGT~TGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT TTGTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT TTGTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT TTGTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGK T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~STT T~GTGT~CGT NNNNNN~NNN T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT ....|....| 535 ~CGACGCGC~ ~NNNNNNNN~ ~CGACGCGCG ~CGACGCGC~ ~CGACGCGCG ~CGACGCGC~ ~NNNNNNNN~ ~CGACGCGCG ~CGACGCGC~ ~CGGCGCGC~ ~MTACGCGC~ ~CGACGCGCG ~CGGCGCGC~ ~CGACGCGC~ ~CGACGCGCG ~CGACGCGC~ ~CGACGCGCG ~CGACGCGC~ ~CGACGCGC~ ~CTA~GCGTT ~CGGCGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CAACGCGCG ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~~~ACACGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGCG ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGCG ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CAACGCGCG ~MSACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGGCGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CNACTCAC~ ~CGACGCGC~ ~NNNNNNNN~ ~CGACGCGC~ ~CGGCGCGC~ ~CGACGCGC~ ....|....| 545 ~ANAACGCN~ ~NNNNNNNN~ CAGAATGCG~ ~AGAACGCG~ CAGAATGCG~ ~AGAACGCG~ ~NGAACGCG~ CAGAATGCG~ ~AGAACGCG~ ~GTGATGCG~ ~AGAACGCG~ CAGAATGCG~ ~GTGATGCG~ ~AGAACGCG~ CAGAATGCG~ ~AGAACGCG~ CAGAATGCG~ ~AGAACGCG~ ~AGAACGCG~ GAGAATGCG~ ~GTGATGCG~ ~AGAATGCG~ ~AGGACGCG~ ~AGGGCGCG~ ~GGAACGCG~ ~ARAACGCG~ ~AGAACGCG~ ~AGAACGCG~ CAGAATGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~~~~~~~~~~ ~AGAACGCG~ ~AGAACGCG~ CAGAATGCG~ ~ANAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAATGCG~ CAGAATGCG~ ~AGAACGCG~ ~AGRACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAATGCG~ CAGAATGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~GTGATGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAATGCG~ ~AGAACGCG~ ~AGAATGCG~ ~ASAACGCG~ ~AGAACGCG~ ~ACAACGCG~ ~AGAACGCG~ ~NNNNNNNN~ ~AGAACGCG~ ~GTGATGCG~ ~AGAACGCG~ ....|....| 555 CTGCG~~~TA NNNNN~~~NN CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA TTGGG~TGWA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~TGTA CTGCG~~~TA CGAGG~~~TG CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~TGTA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA ~~~~~~~~~~ CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTTCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~TGTA CTGCG~~~TA CTGCG~~~TA NNNNN~~~NN CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA ....|....| 565 CACGTTTTTT NNNNNTTTTW CTCGTT~~~~ CACGTTTTTT CTCGTT~~~~ CAAGTTTTTT CATTTTTTTT CTCGTT~~~~ CACGTTTTTT ~~CGTT~~~~ CACGTTTTTT CTCGTT~~~~ ~~CGTT~~~~ CAAGTTTTTT CTCGTT~~~~ CACGTTTTTT CTCGTT~~~~ CACGTTTTTT CACGTTTTTT CTCTCCAT~~ ~~CGTT~~~~ ~~CGTTTTTT CACGTTTTTT CACGTTTTT~ CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CTCGTT~~~~ CGCGTTTCTT CACGTTTTTT CGCGTTTCTT CACGTTTTTT CACGTTTTTT ~~~~~~~~~T CASGTTTTTT CACGTTTTTT CTCGTT~~~~ CACGTTTTTT CACGTTTTTT CACGTTTTAT CACGTTTTTT ~ATNNNNNN~ CTCGTT~~~~ CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT ~~CGTTC~~~ CTCGTT~~~~ CACGTTATTT CACGTTTTTT CGCGTTTCTT CACGTTTTTT ~~CGTT~~~~ CACGTTTTTT CACGTTTTTT ~~CGTTT~~~ CAAGTTTTTT ~~CGTTT~~~ CACGTTTTTT CACGTTTTTT CGCGTTTNTT CACGTTTTTT NNNNTTTTTT CACGTTTTTT ~~CGTT~~~~ CACGTTTTTT 195 ....|....| 575 T~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~C ~~~~~~~~~~ ~~~~~~~~~C TCT~~~~~~~ CCT~~~~~~~ ~~~~~~~~~C ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~C ~~~~~~~~~~ TTTTTTT~~~ ~~~~~~~~~C ~~~~~~~~~~ ~~~~~~~~~C ~~~~~~~~~~ TCT~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~C TT~~~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~C ~~~~~~~~~~ ~~~~~~~~~~ TTTTTCCTTT ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~C ~~~~~~~~~~ TTTTTT~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~C ~~~~~~~~~~ TTTTTTTTTT TT~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ CTTT~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ....|....| 585 ~~~~~~AACG ~~~~~~AASR GTTCGTTGCG ~~~~~~AACG GTTCGTTGCG ~~~~~AAACG ~~~~~~AAMR GTTCGTTGCG ~~~~~~AACG ~~~~~~~~CG ~~~~~~AACG GTTCGTTGCG ~~~~~~~~CG G~CAAAAAGG GTTCGTTGCG ~~~~~~AACG GTTCGTTGCG ~~~~~~AACG ~~~~~~AACG ~~~~~~~~~~ ~~~~~~~~CG ~~~~~~~~~~ ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG GTTCGTTGCG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG GTTCGTTGCG ~~~~~~AACG ~~~~~~AACG TTTT~~AACG ~~~~~~AACG ~~~~~~NNNA GTTCGTTGCG ~~~~~~~~~~ ~~~~~AAACG ~~~~~~AACG ~~~~~~AACG ~~~~~AAAC~ GTTCGTTGCG ~~~~~~AACG TTTT~WAACG ~~~~~~AACG ~~~~~~AACG ~~~~~~~~CG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACA ~~~~~AAACG ~~~~~~~~~A ~~~~~~~~~~ ~~~~~~AACG ~~~~~~WWCK ~~~~~~AACG ~~~~~~AACG ~~~~GTAACG ~~~~~~~~CG ~~~~~~AACG ....|....| 595 AA~~~~~AAA AA~~~~~AAA AA~~~CGCCG AA~~~AAAAA AA~~~CGCCG AA~~~~~AAA AA~~~~GAMA AA~~~CGCCG AA~~~~~AAA AA~~~CGAAA AA~~~~AAAA AA~~~CGCCG AA~~~CGAAA AA~~~AGAGA AA~~~CGCCG GA~~~~AAAA AA~~~CGCCG AA~AAAAAAA AA~~~~AAAA ~~~~~~~~~~ AA~~~CGAAA ~~~~~~~~~A AATAAAAAAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~CGCCG AA~~~~AGAA AG~~~~~AAA AA~~~~AGAA AG~~~~~AAA AA~~~~~AAA AA~~~~AGAA AA~~~~~AAA AG~~~~~AAA AA~~~CGCCG AA~AAAAAAA AA~~~~~AAA AA~~~AAAAA AA~~~~~AAA AA~~~~~AAA AA~~~CGCCG AA~CGGAAAA AAACGAAAAA AATAAAAAAA GA~~~~~AAA ~A~~~~GAAA AA~~~CGCCG AA~~~~~AAA AA~~~~~AAA AA~~~~AGAA AA~~~~~AAA AA~~~CGAAA AA~~~~~AAA AA~~~~~AAA AAA~~C~~AA AA~~~~~AAA AA~~~CAAAA AA~CGGAAAA AA~~AAGAAA AA~~~~AGAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~CGAAA AA~~~~~AAA ....|....| 605 A~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC A~~CA~CACC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC A~~CA~CGCC ~~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC A~~CAACACC ~~~~~~~~~~ A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~TC A~~CA~~~CC A~~CA~~~CC A~~CA~~~TC ~~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ~~~CA~CACC ~~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~TC A~~CA~~~CC A~~CA~CACC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC G~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ....|....| 615 ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC RCCG~~~~TC ACCG~~~~TC GCCG~~~~TC ACCG~~~~TC ACCG~~~~TC GCCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ~~~~~~~~~~ GCCG~~~~TC GCCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCT~~~~TC ACCG~~~~TC ACCT~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACMG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC GCCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC CCCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC GCCG~~~~TC ACCG~~~~TC ....|....| 625 G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAGCACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT ~~~~~~~~~~ G~AAAACACT GAAAAACACA G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACC G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACG G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACGCT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~GAGACACT G~AAAACACT G~AAAACACT G~AAAACACT ....|....| 635 G~~~TTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~~TTGT~~ ~~~~~~~~~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~A G~~~TTAT~~ G~~~TTAT~A G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~A G~~~TTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TCGT~~ G~~GTTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~A G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTATAT G~~~TTAT~~ G~~~TTAT~~ G~~~TKAT~~ G~~~TTAT~~ G~~~TTAT~A G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ Appendix 3: Sequence data MW99134 MW99135 MW99163 MW99164 MW99174 MW99196 MW99203 MW99226 MW99240 MW99247 MW99274 MW99276 MW99286 MW99292 MW99301 MW99303 MW99309 MW99319 MW99341 MW99353 MW99372 MW99374 MW99382 MW99393 MW99406 MW99407 MW99408 MW99413 MW99448 MW99465 MW99471 MW99479 MW99501 MW99508 MW99514 MW99536 MW99537 MW99546 MW99550 MW99552 MW99559 MW99565 MW99591 MW99605 MW99608 MW99612 MW99613 OK96022 WE00002 WE02431 WE02451 WE02454 WE02491 WE02531 WE02534 WE02535 WE02591 WE02614 WE02621 WE02661 WE02662 WE02671 WE02674 WE02677 WE85001 WE94001 ....|....| 485 TCGGTGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ NNNNNNNN~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCKGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGTGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ TCGGCGAG~~ ....|....| 495 CGGGGCGTGC CGGGGCGTGC CGGGGCG~GC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC NNNNNNNNNN CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC CGGGGCGTGC ....|....| 505 ACGC~~AC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ NNNN~~NN~~ ACGC~~AC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ RCGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ GCGC~~GC~~ GCGC~~GC~~ ACGC~~AC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~AC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ ACGC~~GC~~ Internal transcribed spacer 2 (ITS-2) ....|....| 515 ~GT~CGAA~C ~GT~CGAA~C ~NN~NNNN~N ~GT~CGAA~C ~GT~CGAA~C ~GTTCGAAAC ~GT~CGAA~C ~GT~CGAA~C ~GT~TGAA~N ~GT~CGAA~C ~GT~CGAW~C ~GT~CGAA~C ~GT~CGAA~C ~NN~NNNN~N ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CKAA~C ~GT~CKAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CCAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CRAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ~GT~CGAA~C ....|....| 525 T~GTGT~CGT T~GTGT~CGT N~NNNN~NNN T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGC T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT NNNNNN~NNN K~GTGT~CGG T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT TTGTGT~CGT K~GTGT~CGK T~GTGT~CGC T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT TTGTGT~CGT TTGTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT TTGTGTTCGT TTGTGT~CGT T~GTGT~CGT TTGTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGC T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGT~CGT T~GTGTTCGT ....|....| 535 ~CGGCGCG~G ~CGACGCGC~ ~NNNNNNNN~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ CAGACGCGC~ ~CKACGCGC~ ~NNACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~NNNNNNNN~ ~SGAAGCGCA ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~SKACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGCG ~CGACGC~CG ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CKACGCGC~ TCGACGCGCG ~CGACGCGCG ~CGACGCGC~ ~CGACGCGCG ~CGGCGCGC~ ~CGACGCGC~ ~CGGCGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGRCGGGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGSGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ~CGACGCGC~ ....|....| 545 CAGAGTGCG~ ~AGAACGCG~ ~NNNNNNNN~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~NNNNNNNN~ ~AGAACGCG~ ~AGAATGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGGACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGCACGCG~ ~AGAACGCG~ ~AGCACGCG~ CAGAATGCG~ CAGAATGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ CAGAATGCG~ CAGAATGCG~ ~AGAACGCG~ CAGAATGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGCACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ ~AGAACGCG~ TAGANNNNN~ ....|....| 555 CTGCG~~~TA CTGCG~~~TA NNNNN~~~NN CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TG NNNNN~~~NN CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGSG~~~TA CTGCG~~~TA CTGCG~~~TG CTGCG~~~TG CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA YTGCG~~~TG CTGCG~~~TA CTGCG~~~TA CTGCG~~~TA CTGCG~~~TG CTGCG~~~TA CTGCG~~~YA CTGCG~~~TA NNNNN~~~NN ....|....| 565 ~~CGTGTTTT CACGTTTTTT NNNNNNNNN~ CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CGCGTTTCTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CA~~~~~~~~ NNNNNNNNNN CACGTTATTT ~~CGTTTCTT CACGTTTTTT CACGTTTTTT CACGTTCTTT CGCGTTTCTT CACSTTTYTT CACGTTTTTT CACGTTTTT~ CA~~~~~~~~ CA~~~~~~~~ CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CGCGTTTCTT CACGTTTTTT CGCGTTTTTT CACGTTTTTT CACGTTTTTT CTCGTTGTT~ CTCGTTGTT~ CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CTCGTT~~~~ CTCGTT~~~~ CACGTTTTTT CTCGTT~~~~ CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTTTTT CACGTTYTTT CACGTTTTTT CACGTTTTT~ CA~~~~~~~~ CACGTTTTTT CACGTTTTTT CACGTTTTTT CA~~~~~~~~ CACGTTTCTT CACGTTTTT~ CACGTTTTTT NNNNNNNNNN 196 ....|....| 575 TGTTTATT~~ TT~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TNTG~GGAA~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ TT~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ C~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ TCG~T~~~~~ TT~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~C ~~~~~~~~~C ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~C ~~~~~~~~~C T~~~~~~~~~ ~~~~~~~~~C TTT~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ T~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ CTTTT~~~~~ C~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ....|....| 585 ~~~~~~AAC~ ~~~~~~AACG ~~~~~~NNNN ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~~~~~ ~~~~~~NNNN ~~~~~~AATA ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACT ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG GTTCGTTGCG GTTCGTTGCG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~GTAACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG GTTCGTTGCG GTTCGTTGCG ~~~~~~AACG GTTCGTTGCG ~~~~~~AACG ~~~~~~AACG ~~~~~~AGCG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~CAACG ~~~~~~~~~~ ~~~~~~AACG ~~~~~~AACG ~~~~~~AACG ~~~~~~~~~~ ~~~~~~AAAG ~~~~~~AACG ~~~~~~AACG ~~~~~~NNNN ....|....| 595 AA~~~~~AAA AA~~~~~AAA NN~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~AGAA AA~~~~~AAA AA~~~~~AAM AA~~~~~AAA ~~~~~AAAAA NN~~~~~NNC AA~~~~AAAA ~~~~~~~AAA AA~~~~~AAW AA~~~~~AAA AA~~~~AAAA AA~~~~AGAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA ~~~~~~AAAA ~~~~~~AAAA AA~~~~~AAA AA~~~AAAAA AA~~~~~AAA AA~~~~~AAA AAA~~~GAAA AA~~~~~AAA AA~~~~~AAA AG~~~~~AAA AA~~~~~AAA AA~~~CGCCG AA~~~CGCCG GA~~~~~AAA AA~~~~~AAA GA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~CGCCG AA~~~CGCCG AA~~~~~AAA AA~~~CGCCG AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~TAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA AAG~~~~AAA ~~~~~AAAAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA ~~~~~~AAAA AA~~~~~AAA AA~~~~~AAA AA~~~~~AAA NN~~~~~NNN ....|....| 605 ~~~CAACACC A~~CA~~~TC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~TC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CC~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~TC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CM~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC ~~~CA~~~CC A~~CACCACC A~~CA~~~CC A~~CACCACC G~~CA~~~CC A~~CA~~~TC A~~CA~~~TC A~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC ~~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~TC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC A~~CA~~~CC N~~NN~~~NN ....|....| 615 ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC MCYG~~~~TM ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC CCCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCG~~~~TC ACCGTCTGTC ACCG~~~~TC ACCG~~~~TC NNNN~~~~NN ....|....| 625 G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AACACTST G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACRCT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~GAGACACT G~AAAACACT G~AAAACACT K~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT G~AAAACACT C~GAAACACT G~AAAACACT G~AAAACACT N~NNNNNNNN ....|....| 635 G~~~TTAT~~ G~~~TTAT~~ G~T~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~A G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~T~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTRT~~ G~~~TTAT~A G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~A G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~~GTTAT~~ G~~~TTAT~~ G~TGTTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ G~~~TTAT~~ N~~~NNNN~~ Appendix 3: Sequence data AD98001 AD98009 AD98012 AD98018 AD98024 AD98036 DS00001 DS01001 JC00029 JC00039 JC00040 JC00042 JC00043 JC00045 JC00051 JC00055 JC00061 JC00063 JC01014 JM00001 MW00015 MW00024 MW00032 MW00051 MW00058 MW00064 MW00072 MW00076 MW00101 MW00102 MW00110 MW00116 MW00127 MW00129 MW00151 MW00176 MW00177 MW00178 MW00179 MW00185 MW00189 MW00226 MW00231 MW00232 MW00234 MW00262 MW00267 MW00269 MW00290 MW00302 MW00316 MW00326 MW00328 MW00330 MW00347 MW00348 MW00393 MW00409 MW00412 MW00444 MW00452 MW00469 MW00498 MW00504 MW00517 MW00525 MW00530 MW00539 ....|....| 645 ~NNN~~~~NN ~ATT~~~~AT TATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~GT TATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~RTT~~~~GT ~ATT~~~~AT ~AATA~~TAT ~ATT~~~~GT ~ATT~~~~GT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATTATTTAT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~~~~~~~~~T ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~GT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~GT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ....|....| 655 NNNNNN~~NN TAATTG~~AA CAATTG~~AA CAATTGTGAA CAATTG~~AA CAATTG~~AA TAATTG~~AA TAATTG~~AA ~~~TTG~~AA ~~~TTG~~AA TAATTG~~AA ~~~TTG~~AA TAATTG~~AA TAATTG~~AA ~~~TTG~~AA ~~~TTG~~AA ~~~TTG~~AA ~~~TTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA ~~~TTG~~AA CAATTG~~AA CAATCG~~AA CAATCG~~AA TAATCG~~AA TAATTG~~AA ~~~TTGAGAA TAATTG~~AA TGATTG~~AA CAATCG~~AA ~~~TTG~~AA CAATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA CGATTG~~AA TAATTG~~AA CAATTG~~AA TAATTG~~AA TAATTA~~AA TGATTG~~AA TAATTG~~AA ~~~TTGAGAA ~~~TCG~~AA CAATCG~~AA ~~~TTGA~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA CAATTG~~AA TAATTG~~AA TAATTG~~AA ~~~TTG~~AA TAATTG~~AA CAATTG~~AA ~~~ACG~~AA TAATCG~~AA ~~~TCG~~AA TTGTTG~~AA ~~~TCG~~AA ~~~TTG~~AA TAATTG~~AA ....|....| 665 N~NN~~~NNN T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA TATT~~~ATA C~TA~~~ATA T~TA~TAATA T~TA~~~ATA A~TA~~~ATA T~TA~~~ATG TATA~~~ATA A~TA~~~ATA T~TA~~~ATA T~TA~~~ATA A~TA~~~ATA T~TA~~~ATG A~TA~~~ATA A~TA~~~ATA T~YA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TG~~~AAT T~TA~~~ATA T~TG~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TR~~~ATA T~TA~~~ATG T~TA~~~ATA T~TA~~~ATT T~TA~~~ATA T~TA~~~ATA T~TA~~~ATT T~TA~~~ATT T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TG~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~CA~~~ATA T~TA~~~ATA TG~A~~~ATA T~TA~~~ATA T~TA~~~ATT T~TA~~~ATA C~TA~~~ATA T~TA~~~ATA C~TA~~~ATA A~TG~~~ATA T~CA~~~ATA C~TA~~~ATA T~TA~~~ACG T~TA~~~ATA T~TA~~~ATA T~TA~~~ATG T~TG~~~ATA A~TG~~~ATA T~TA~~~ATA Internal transcribed spacer 2 (ITS-2) ....|....| 675 N~~~~~NN~N G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A CA~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A GAA~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A GA~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A GAA~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A GAG~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~ACA~GA G~~~~~ACAA T~~~~~AC~A ....|....| 685 NNNNNNNNNN GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC ....|....| 695 NNNNNNNNNN TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACKTCCR TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG ....|....| 705 NNNNNNNNNN AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGTGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT ....|....| 715 NNNNNNNNNN CGTCGACGCC CGTCGACGCC CGTCGACGCC TTTCAACCCC CGTCGACGCC CGTCNACCCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC ....|....| 725 NNNNNN~~~N GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C CACNTG~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGCA~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C KACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C 197 ....|....| 735 NNNNNNNNNN CGATTGATTT CGATTGATTT CGATTGATTT CSATNGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATCT CGATTGATCT CGATTGATTT CGATTGATTT CGTTTGATTT CGTTTGATTT CGATTGATCT CGATTGATTT CGATTGATCT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATCGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATCT CGATTGATCT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT ....|....| 745 NNNNNNNNNN CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCTGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CTGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA ....|....| 755 NNNNNNNNNN GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCTGAGTCG GGCGGAGTCC GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTC~ GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCTGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCTGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG ....|....| 765 NNNNNNNNNN TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCNCTNNN TTGCGCTGAC TTGCGCTTAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC ....|....| 775 NNNNNNNNNN GGATATCGCG GGATATCGCG GGATATCGCG NNNNNNNNNN GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG NGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG ....|....| 785 NNNNNNNNNN TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA NNNNNNNNNN TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTNNNNNNN TCTGCCTCNN TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAN TCTGCCTCAA TCTGCCTCAA TCTGCCTCAW TCTGCMTCAA TCTGCCTCAN TCTGCCTCAA TCTGCCTCAA TCTGCCTCNN TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA ....|.. 795 NNNNNNN TTTTTTA TTTTTTA TTTTTTA NNNNNNN TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTN ATTTTTA TTTTTTA TTTTTTA TTTTT~A TTTTTTA TTTTTTA ATTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA NNNNNNN NNNNNNN TTTTTTA TTTTTTN ATWTATA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA NNNNNNN TTTTTTA TTTTTTN ATTTTTA TTNTATN NNNNNNN TTTTTTA TTTTTTA NNNNNNN TTTTTTA TTTTTTA TTTTTCN TTTTTTA TTTTTTA TTTTTTA TTTTTTA ATTTTTA TTTTTTA TTTTTNN ATTTTTA ATTTTTA TTTTT~A TTTTTTN Appendix 3: Sequence data MW00547 MW01001 MW01011 MW01014 MW01018 MW01019 MW01025 MW01034 MW01039 MW01048 MW01053 MW01059 MW01061 MW01083 MW01092 MW01107 MW01116 MW02001 MW02006 MW02025 MW02033 MW02034 MW98009 MW98029 MW98049 MW98079 MW98097 MW98103 MW98129 MW98136 MW98138 MW98139 MW98154 MW98162 MW98170 MW98172 MW98180 MW98185 MW98189 MW98203 MW98205 MW98212 MW98220 MW98228 MW98235 MW98240 MW98261 MW98264 MW98270 MW98278 MW98285 MW98294 MW98313 MW98315 MW99001 MW99006 MW99009 MW99013 MW99014 MW99018 MW99038 MW99047 MW99057 MW99058 MW99094 MW99095 MW99097 MW99105 ....|....| 645 ~GTT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~~~~~~~~~~ ~ATT~~~~GT ~ATT~~~~AT ~ACT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~GTT~~~~AT TATT~~~~AT ~ATC~~~~AT TATT~~~~AT ~ATC~~~~AT ~ATT~~~~AT TATT~~~~AT ~ATT~~~~AT ~ATC~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ACT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~ATT~~~~AT TGTT~~~~AT ~ATTT~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT TATT~~~~AT ~ATT~~~~AT ~~~~~~~~~~ ~ATT~~~~AT ~ATT~~~~AT TATT~~~~AT ~ATT~~~~AT ~ATCAA~TAT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ....|....| 655 TAATTG~~AA TAATTG~~AA ~~~ACG~~AA TAATTG~~AA ~~~ACG~~AA ~~~TTG~~AA ~~~TTG~~AA ~~~ACG~~AA TAATTG~~AA ~~~TTG~~AA TAATTG~~AA ~~~ACG~~AA ~~~TTG~~AA ~~~TCG~~AA ~~~ACG~~AA TAATTG~~AA ~~~ACG~~AA ~~~TTG~~AA ~~~TTG~~AA ~~~~~~~~~~ ~~~TTG~~AA ~~~TCG~~AA CAAATG~~AA ~~~TTGAGAA TAATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA ~~~ACG~~GA CAATTG~~RA TAATTG~~AA CAATTG~~AA TAATTG~~AA TAATTG~~AA CAATTG~~AA TAATTG~~AA TAATTG~~AA ~~~ACG~~GA TAATTG~~AA TAATTG~~AA CAATTG~~AA TAATTG~~AA ~~~TCG~~AA ~~~ACG~~AA ~~~TTGA~AA CAATTG~~AA CAAATG~~AA ~~~TTGA~AA ~~~TTG~~AA ~~~ACG~~AA TAATTG~~AA CAATTG~~AA CAATTG~~AA TAATTG~~AA ~~~TTG~~AA TAATTG~~AA TAATTG~~AA ~~~TTG~~AA ~~~TTG~~AA ~~~~~~~~AA ~~~TTGA~AA ~~~TTG~~AA CAATTG~~AA TAATTG~~AA ~AATTG~~AA CAATTG~~AA ~~~TTG~~AA TAATTG~~AA ....|....| 665 T~TA~~~ACA T~TA~~~ATA T~TA~~~ACG T~TA~~~ATG T~TA~~~ACG T~TA~~~ATA A~YA~~~ATA T~TA~~~ACG T~TA~~~ATA T~TA~~~ATG T~TA~~~ATA T~TA~~~ACG T~TA~~~ATG T~CA~~~ATA T~TA~~~ACG T~TA~~~ATA T~TA~~~ACG T~TA~~~ATA T~CA~~~ATA ~~~~~~~~~~ T~TA~~~ACG T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ACG T~TA~~~ATA TTCA~~~ATA T~TA~~~ATA TTCA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ACG T~TA~~~ATA T~TA~~~ATA T~TG~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ACG TTCA~~~ATA T~TG~~~ATA T~TA~~~ATA TTCA~~~ATA T~TA~~~ATG T~TA~~~ACG T~TA~~~ATA T~TG~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATG T~TA~~~ATA T~TA~~~ATA T~CA~~~ATA T~TA~~~ACA T~TA~~~ATA TTCA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TG~~~ATA T~TA~~~ATG T~TA~~~ATA Internal transcribed spacer 2 (ITS-2) ....|....| 675 N~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A ~~~~~~~C~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A GAA~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AA~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~ACAA A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A TA~CACAC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AA~A A~~~~~AC~A ....|....| 685 GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC ....|....| 695 TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG ....|....| 705 AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT ....|....| 715 CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC ....|....| 725 GACNTG~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C GACGCA~~~C GACGTG~~~C GACGTA~~~C GACGCA~~~C GACGCA~~~C GACGTA~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C GACGCA~~~C GACGTA~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGCA~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGCA~~~C GACGTG~~~C 198 ....|....| 735 CNATNGATTT CGATTGATTT CGATTGATTT CGATTGATCT CGATTGATTT CGATTGATTT CGTTTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CG~TTCA~CG CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGGTTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGACAT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATCT ....|....| 745 CGGTCNCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTT~CGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CTGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA ....|....| 755 GGCGGANTCN GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGTAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG ....|....| 765 TTGCNCTGCN TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC YTGCGCTGAC TTGCGCTGAC ....|....| 775 NGNNNTCGNG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATNNNNNN GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCKCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG ....|....| 785 TNTGCCTNAA TCTGCCTCAA TCTGNNNNNN TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA THTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA NNNNNNNNNN TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA ....|.. 795 TTTTTTN TTTTTNN NNNNNNN TTTTTTA ATTTTTA TTTTTTA TTTTTTA ATTTTTA TTTTTTA ATTTTTA TTGTTTA ATTTTTA ATTTTTA TTTTTCN ATTTTTA TTTTT~A ATTTTTA TTTTTTA TTTTTTA ATTTTTA ATTTTTA ATTTTTA TTTTTTA ATTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA NNNNNNN TTTTTTA TTTTTTA TTTTTTA TTNNNNN TTTTTTA TTTTTTA TTTTTTA TTTTTTA ATTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA ATTTTGN ATTTTTN TTTTTTA TTTTTTA TTTTTTA TTTTTTA ATTTTTA ATTTTTA TTTTYTA TTTTTTA TTTTTTA NNNNNNN ATTTTTA TTTTTTA TTTTTTN ATTTTTA TTTTTTA ATTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TNNNNNN ATTTTGA TTTTTTA Appendix 3: Sequence data MW99134 MW99135 MW99163 MW99164 MW99174 MW99196 MW99203 MW99226 MW99240 MW99247 MW99274 MW99276 MW99286 MW99292 MW99301 MW99303 MW99309 MW99319 MW99341 MW99353 MW99372 MW99374 MW99382 MW99393 MW99406 MW99407 MW99408 MW99413 MW99448 MW99465 MW99471 MW99479 MW99501 MW99508 MW99514 MW99536 MW99537 MW99546 MW99550 MW99552 MW99559 MW99565 MW99591 MW99605 MW99608 MW99612 MW99613 OK96022 WE00002 WE02431 WE02451 WE02454 WE02491 WE02531 WE02534 WE02535 WE02591 WE02614 WE02621 WE02661 WE02662 WE02671 WE02674 WE02677 WE85001 WE94001 ....|....| 645 ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT TATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT TATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATTA~TTAT ~ATT~~~~AT ~ATT~~~~AT TATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATCAA~TAT TATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~GTT~~~~GT ~GTT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~GTT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~ATT~~~~AT ~AATA~~TAT ~ATT~~~~AT ~RTT~~~~GT ~ATT~~~~AT ~ATT~~~~AT ~NNN~~~~NN ....|....| 655 ~~~TTG~~AA TAATTG~~AA ~~~TTG~~AA TAATTG~~AA CAATTG~~AA TAATTG~~AA TAATTG~~AA CGATTG~~AA CAATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA CAATTG~~AA CGWTTR~~AA ~~~TTG~~AA ~~~TTG~~AA TAATTG~~AA TAATTG~~AA CGATTG~~AA CAATTG~~AA TAATTG~~AA CGATTG~~AA TAATTG~~AA CAATTG~~AA CAATTG~~AA TAACTG~~AA TAATTG~~AA TAATTG~~AA ~AATTG~~AA CAATTG~~AA TAATTG~~AA CAATTG~~AA TAATTG~~AA CAATTGTGAA ~~~ACG~~AA ~~~ACG~~AA ~~~TTGA~AA TAATTG~~AA ~~~TTGA~AA CAATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA ~~~ACG~~AA ~~~ACG~~AA TAATTG~~AA ~~~ACG~~AA AAATTA~~AA TAATTG~~AA TAATTA~~AA TAATTG~~AA TAATTG~~AA CAATCG~~AA TAATYG~~AA TAATTG~~AA CAATTG~~AW CGATTG~~AA TAATTG~~AA CAATTGTGAA TAATTG~~AA CRATTG~~AA TAATTG~~AA TAATTG~~AA TAATTG~~AA NNNNNN~~NN ....|....| 665 T~TG~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA C~TAATAATA T~TA~~~ATA T~TA~~~ATA T~TAAT~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TG~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TAAT~ATA T~TA~~~ATA T~TG~~~ATA T~TG~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA TTCA~~~ATA T~TA~~~ATA T~TA~~~ACG T~TA~~~ACG T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TG~~~ATA T~TA~~~ATA T~TA~~~ATA TATA~~~ATA TATA~~~ATA T~TA~~~ACG T~TA~~~ACG T~TA~~~ATA T~TA~~~ACG T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ATA T~TA~~~ACA T~TA~~~ATA T~TA~~~ATA T~TG~~~ATA T~TG~~~ATA T~TA~~~ATA T~TG~~~ATA T~TA~~~ATA T~TG~~~ATA T~TA~~~ATT T~TA~~~ATA T~TA~~~ATA N~NN~~~NNN Internal transcribed spacer 2 (ITS-2) ....|....| 675 TAA~~~AC~A G~~~~~AC~A AN~~~~ACCA A~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A ATAA~~ACCA G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~MC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A A~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A G~~~~~AC~A A~~~~~AC~A G~~~~~AC~A N~~~~~NN~N ....|....| 685 GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTAGGCGGAC GTANGCGGAC GTAGGCGGAC NNNNNNNNNN ....|....| 695 TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG TCGACGTCCG NNNNNNNNNN ....|....| 705 AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT AAGGGCGCGT NNNNNNNNNN ....|....| 715 CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACTCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACNCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC CGTCGACGCC NNNNNNNNNN ....|....| 725 GACGTA~~~C GACGTG~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACNTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTACTAC GACGTA~~~C GACGTACTAC GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTA~~~C GACGTA~~~C GACGTA~~~C CACCTA~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C GACGTG~~~C NNNNNN~~~N 199 ....|....| 735 CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATCT CGATTGATTT CGATCGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATCGATTT CGATTGATCT CGATTGATTT CGATTGATTT CGATTGATCT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGGTTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATCT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATCT CGATTGATCT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATTT CGATTGATCT CGATTGATTT NNNNNNNNNN ....|....| 745 CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCKCGGA CGGTCGCGGA CGGTCGCTGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCTGA CGGTCGCTGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGNA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CNGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCKGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA CGGTCGCTGA CGGTCGCGGA CGGTCGCGGA CGGTCGCGGA NNNNNNNNNN ....|....| 755 GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCTGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG GGCGGAGTCG NNNNNNNNNN ....|....| 765 TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGSTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTNAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCCGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC NNNNNNNNNN TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC TTGCGCTGAC NNNNNNNNNN ....|....| 775 GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATSGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG NNNNNNNNNN GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG NGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG GGATATCGCG NNNNNNNNNN ....|....| 785 TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAN TCTGCCTCAA TCKGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TYKGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCMTSAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA NNNNNNNNNN TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA TCTGCCTCAA NNNNNNNNNN ....|.. 795 ATTTTTA TTTTTTA NNNNNNN TTTTTTA TTTTTTA TTTTTTN TTTTYTA TTTTTTA NNNNNNN TTTTTTA TTKTYTA TTTTTTA TTTTTTA TTTTNNN TTTTTTA ATTTTTA TTTTTTA TTKTYTA TTTTTTA TTTTTTA TTTTTTA TTKTYCA TTTTTTA TTKTTTA TYYTTTA TTTTTTA TTTTTTA NNNNNNN TTTTTTA TTTTTTA TTTTTNN TTTTTTA TTTTTTA TTTTTTA ATTTTKA ATTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA NNNNNNN TTTTTTA TTTTTTA TTTTTTA ATTTTTA ATTTT~A TTTTTTA ATTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TNNNNNN TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA TTTTTTA ATTTT~A TTTTTTA NNNNNNN Legend Plate 1 & 2. Agrodiaetus-wings Plate 1 Fig. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 Code AD 98001 AD 98009 DS 00001 DS 00002 DS 00003 DS 00004 DS 00005 DS 01001 DS 95001 JC 00040 JC 00045 JC 01014 JM 00001 MW 00015 MW 00032 MW 00059 MW 00101 MW 00127 MW 00129 MW 00176 MW 00179 MW 00226 MW 00231 MW 00234 MW 00262 MW 00269 MW 00330 MW 00347 MW 00348 MW 00393 MW 00409 MW 00426 MW 00498 MW 00539 MW 00547 MW 01014 MW 01053 MW 01107 MW 98009 MW 98079 MW 98097 MW 98103 MW 98136 MW 98154 MW 98162 MW 98170 MW 98172 MW 98189 MW 98203 MW 98240 Plate 2 M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M F M M M M M M M M M M M M M M M M M M Species Fig. Code Species 1 MW 98261 M Agrodiaetus schuriani Agrodiaetus surakovi 2 MW 98284 M Agrodiaetus sigberti Agrodiaetus pseudactis 3 MW 98285 M Agrodiaetus sigberti Agrodiaetus poseidonides 4 MW 98294 M Agrodiaetus guezelmavi Agrodiaetus damone 5 MW 98313 M Agrodiaetus sertavulensis Agrodiaetus damocles 6 MW 99006 M Agrodiaetus firdussii Agrodiaetus phyllides 7 MW 99009 M Agrodiaetus iphigenia Agrodiaetus dagmara 8 MW 99057 M Agrodiaetus merhaba Agrodiaetus iphigenides 9 MW 99058 M Agrodiaetus artvinensis Agrodiaetus actinides 10 MW 99095 M Agrodiaetus huberti Agrodiaetus aroaniensis Agrodiaetus nephohiptamenos 11 MW 99105 M Agrodiaetus demavendi 12 MW 99164 M Agrodiaetus interjectus Agrodiaetus admetus 13 MW 99196 M Agrodiaetus ripartii Agrodiaetus fabressei 14 MW 99203 M Agrodiaetus turcicus Agrodiaetus demavendi 15 MW 99240 M Agrodiaetus altivagans Agrodiaetus hamadanensis 16 MW 99274 M Agrodiaetus dantchenkoi Agrodiaetus elbursicus 17 MW 99286 M Agrodiaetus kurdistanicus Agrodiaetus darius 18 MW 99289 M Agrodiaetus sekercioglui Agrodiaetus paulae 19 MW 99309 M Agrodiaetus baytopi Agrodiaetus gorbunovi 20 MW 99341 M Agrodiaetus pierceae Agrodiaetus rovshani 21 MW 99353 M Agrodiaetus altivagans Agrodiaetus cyaneus 22 MW 99374 M Agrodiaetus zapvadi Agrodiaetus femininoides 23 MW 99393 M Agrodiaetus antidolus Agrodiaetus alcestis 24 MW 99406 M Agrodiaetus antidolus Agrodiaetus firdussii 25 MW 99408 M Agrodiaetus hopfferi Agrodiaetus klausschuriani 26 MW 99413 M Agrodiaetus firdussii Agrodiaetus iphidamon 27 MW 99448 M Agrodiaetus cyaneus Agrodiaetus pseudoxerxes 28 MW 99465 M Agrodiaetus kanduli Agrodiaetus posthumus 29 MW 99471 M Agrodiaetus dantchenkoiXmenalcas Agrodiaetus phyllis 30 MW 99479 M Agrodiaetus turcicola Agrodiaetus erschoffii 31 MW 99494 M Agrodiaetus menalcas Agrodiaetus caeruleus 32 MW 99501 M Agrodiaetus putnami Agrodiaetus caeruleus 33 MW 99508 M Agrodiaetus ninae Agrodiaetus valiabadi 34 MW 99565 M Agrodiaetus tankeri Agrodiaetus dizinensis 35 MW 99591 M Agrodiaetus humedasae Agrodiaetus birunii 36 MW 99613 M Agrodiaetus damon Agrodiaetus ripartii 37 WE 00002 M Agrodiaetus glaucias Agrodiaetus ainsae 38 WE 02451 M Agrodiaetus tenhageni Agrodiaetus fulgens 39 WE 02454 M Agrodiaetus mofidii Agrodiaetus carmon 40 WE 02531 M Agrodiaetus zarathustra Agrodiaetus lycius 41 WE 02535 M Agrodiaetus lorestanus Agrodiaetus ernesti 42 WE 02536 M Agrodiaetus damalis Agrodiaetus iphicarmon 43 WE 02591 M Agrodiaetus peilei Agrodiaetus wagneri 44 WE 02612 M Agrodiaetus karindus Agrodiaetus poseidon 45 WE 02671 M Agrodiaetus femininoides Agrodiaetus actis Agrodiaetus maraschi 1a 1c 2 3 4 5 Agrodiaetus menalcas 1b 1d Agrodiaetus hopfferi 6 7 8 9 10 Agrodiaetus mithridates : : : : : a & b: upperside; c & d: underside; a & c: forewing; b & d:hindwing Agrodiaetus theresiae 201 Index Plate 1 & 2. Agrodiaetus-wings Index A-H Species Plate Fig. Code actinides 1 9 DS 95001 actis 1 45 MW 98162 admetus 1 12 JC 01014 ainsae 1 37 MW 01053 alcestis 1 23 MW 00231 altivagans 2 15 MW 99240 altivagans 2 21 MW 99353 antidolus 2 23 MW 99393 antidolus 2 24 MW 99406 aroaniensis 1 10 JC 00040 artvinensis 2 9 MW 99058 baytopi 2 19 MW 99309 birunii 1 35 MW 00547 caeruleus 1 31 MW 00409 caeruleus 1 32 MW 00426 carmon 1 39 MW 98009 cyaneus 1 21 MW 00179 cyaneus 2 27 MW 99448 dagmara 1 7 DS 00005 damalis 2 42 WE 02536 damocles 1 5 DS 00003 damon 2 36 MW 99613 damone 1 4 DS 00002 dantch.Xmenal. 2 29 MW 99471 dantchenkoi 2 16 MW 99274 darius 1 17 MW 00101 demavendi 1 14 MW 00015 demavendi 2 11 MW 99105 dizinensis 1 34 MW 00539 elbursicus 1 16 MW 00059 ernesti 1 41 MW 98097 erschoffii 1 30 MW 00393 fabressei 1 13 JM 00001 femininoides 1 22 MW 00226 femininoides 2 45 WE 02671 firdussii 1 24 MW 00234 firdussii 2 6 MW 99006 firdussii 2 26 MW 99413 fulgens 1 38 MW 01107 glaucias 2 37 WE 00002 gorbunovi 1 19 MW 00129 guezelmavi 2 4 MW 98294 hamadanensis 1 15 MW 00032 hopfferi 1 48 MW 98189 hopfferi 2 25 MW 99408 huberti 2 10 MW 99095 humedasae 2 35 MW 99591 Index I-Z Sex M M M M M M M M M M M M M M F M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M Species Plate Fig. Code Sex interjectus 2 12 MW 99164 M iphicarmon 1 42 MW 98103 M iphidamon 1 26 MW 00269 M iphigenia 2 7 MW 99009 M iphigenides 1 8 DS 01001 M kanduli 2 28 MW 99465 M karindus 2 44 WE 02612 M klausschuriani 1 25 MW 00262 M kurdistanicus 2 17 MW 99286 M lorestanus 2 41 WE 02535 M lycius 1 40 MW 98079 M maraschi 1 46 MW 98170 M menalcas 1 47 MW 98172 M menalcas 2 31 MW 99494 M merhaba 2 8 MW 99057 M mithridates 1 49 MW 98203 M mofidii 2 39 WE 02454 M nephohiptamenos 1 11 JC 00045 M ninae 2 33 MW 99508 M paulae 1 18 MW 00127 M peilei 2 43 WE 02591 M phyllides 1 6 DS 00004 M phyllis 1 29 MW 00348 M pierceae 2 20 MW 99341 M poseidon 1 44 MW 98154 M poseidonides 1 3 DS 00001 M posthumus 1 28 MW 00347 M pseudactis 1 2 AD 98009 M pseudoxerxes 1 27 MW 00330 M putnami 2 32 MW 99501 M ripartii 1 36 MW 01014 M ripartii 2 13 MW 99196 M rovshani 1 20 MW 00176 M schuriani 2 1 MW 98261 M sekercioglui 2 18 MW 99289 M sertavulensis 2 5 MW 98313 M sigberti 2 2 MW 98284 M sigberti 2 3 MW 98285 M surakovi 1 1 AD 98001 M tankeri 2 34 MW 99565 M tenhageni 2 38 WE 02451 M theresiae 1 50 MW 98240 M turcicola 2 30 MW 99479 M turcicus 2 14 MW 99203 M valiabadi 1 33 MW 00498 M wagneri 1 43 MW 98136 M zapvadi 2 22 MW 99374 M zarathustra 2 40 WE 02531 M 202 Plate 1. Agrodiaetus-wings Plate 2. Agrodiaetus-wings .. . ... .. ...... ....... .. .. ..... .... ..... ....

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Chromosome differentiation and the radiation of the butterfly subgenus Agrodiaetus (Lepidoptera: Lycaenidae: Polyommatus)a molecular phylogenetic …

Chromosome differentiation and the radiation of the butterfly subgenus Agrodiaetus (Lepidoptera: Lycaenidae: Polyommatus)a molecular phylogenetic …

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