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Cortical Organization of Centrifugal Afferents to the Olfactory Bulb: Mono- and Trans-synaptic Tracing with Recombinant Neurotropic Viral Tracers

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Abstract

Sensory processing is strongly modulated by different brain and behavioral states, and this is based on the top–down modulation. In the olfactory system, local neural circuits in the olfactory bulb (OB) are innervated by centrifugal afferents in order to regulate the processing of olfactory information in the OB under different behavioral states. The purpose of the present study was to explore the organization of neural networks in olfactory-related cortices and modulatory nuclei that give rise to direct and indirect innervations to the glomerular layer (GL) of the OB at the whole-brain scale. Injection of different recombinant attenuated neurotropic viruses into the GL showed that it received direct inputs from each layer in the OB, centrifugal inputs from the ipsilateralanterior olfactory nucleus (AON), anterior piriform cortex (Pir), and horizontal limb of diagonal band of Broca (HDB), and various indirect inputs from bilateral cortical neurons in the AON, Pir, amygdala, entorhinal cortex, hippocampus, HDB, dorsal raphe, median raphe and locus coeruleus. These results provide a circuitry basis that will help further understand the mechanism by which olfactory information-processing in the OB is regulated.

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Acknowledgements

We thank Dr. Lingling Xu (Wuhan Institute of Physics and Mathematics) for maintaining and managing the optical platform. This work was supported by grants from the National Natural Science Foundation of China (31400946, 31671120, 31771197, 31329001, 31771156, 91632303, and 81661148053/H09), the Strategic Priority Research Program of Chinese Academy of Science (XDB32030200), and the National Basic Research Development Program (973 Program) of China (2015CB755600).

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  1. Pengjie Wen and Xiaoping Rao contributed equally to this work.

Authors and Affiliations

  1. Center of Brain Science, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Center for Excellence in Brain Science and Intelligent Technology, Chinese Academy of Sciences, Wuhan, 430071, China

    Pengjie Wen, Xiaoping Rao, Zhijian Zhang, Fan Jia, Xiaobin He & Fuqiang Xu

  2. University of the Chinese Academy of Sciences, Beijing, 100049, China

    Pengjie Wen

  3. College of Life Science, Wuhan University, Wuhan, 430072, China

    Liuying Xu

  4. Divisions of Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan, 430074, China

    Fuqiang Xu

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  1. Pengjie Wen
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Wen, P., Rao, X., Xu, L. et al. Cortical Organization of Centrifugal Afferents to the Olfactory Bulb: Mono- and Trans-synaptic Tracing with Recombinant Neurotropic Viral Tracers. Neurosci. Bull. 35, 709–723 (2019). https://doi.org/10.1007/s12264-019-00385-6

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