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Application of Various Delivery Methods for CRISPR/dCas9

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Abstract

As gene-editing technology has become more and more popular in the life sciences, CRISPR has brought good news to scientific researchers because of its efficiency, convenience, and wide application. Its wide application has also promoted the development of basic scientific research, agriculture, basic medicine, and clinical treatment. However, how the CRISPR/dCas9 system is effectively delivered to the target organs or cells is still unknown. This paper briefly introduces the CRISPR/dCas9 system and then lists some common delivery methods and their characteristics.

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Acknowledgment

We thank the National Key Specialty Construction Project of Clinical Pharmacy (Grant No. 30305030698), the Science & Technology Program of Sichuan Province (Grant Nos. 2009SZ0226, 2014FZ0103, 2015JQO027, 2015ZR0160, 20ZDYF1490, and 20CXTD0043), the Health Department of Sichuan Province Grant Nos. 100491, 120111, and 17ZD038), Sichuan Cancer Hospital (Grant No. YB2019001), Chengdu City Science and Technology Project (Grant No. 11PPYB010SF-289), the Young Scholars Foundation of Sichuan Provincial People’s Hospital (Grant Nos. 30305030606 and 30305030859), the Cadre Health Care Research Project of Sichuan Province (Grant No. 2019-801), and Zambon Pharmaceutical Scientific Research Foundation of the Chengdu Pharmaceutical Association (Grant No. 201905).

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  1. Zhixi Liu and Zhi Liao contributed equally to this work should be considered as co-first author.

Authors and Affiliations

  1. Department of Clinical Pharmacy, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, No. 55, Section 4, South Renmin Road, Chengdu, 610041, Sichuan, China

    Zhixi Liu, Yan Chen & Hongtao Xiao

  2. Personalized Drug Therapy Key Laboratory of Sichuan Province, Chengdu, China

    Zhixi Liu, Lizhu Han, Qinan Yin & Hongtao Xiao

  3. Sichuan Translational Medicine Hospital, Chinese Academy of Sciences, Chengdu, China

    Zhixi Liu, Lizhu Han, Qinan Yin & Hongtao Xiao

  4. Department of Gynecology and Obstetrics, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu, China

    Zhi Liao

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  1. Zhixi Liu
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Liu, Z., Liao, Z., Chen, Y. et al. Application of Various Delivery Methods for CRISPR/dCas9. Mol Biotechnol 62, 355–363 (2020). https://doi.org/10.1007/s12033-020-00258-8

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