Abstract
Rhododendron molle G. Don, an endemic species of the Ericaceae family, possesses valuable medicinal and horticultural values. In this research, R. molle cp genome proved to be a typical quadripartite structure with the length of 200,878 bp. In particular, the lengths of large single-copy region (LSC), small single-copy region (SSC), and inverted repeat regions (IR) were 198,019 bp, 629 bp, and 1117 bp, respectively. Among the 149 unique genes, 97 were protein-coding genes, 44 were tRNA genes, and 8 were rRNA genes, respectively. Leucine was the most representative amino acid (10.663%), while Cysteine was the lowest representative (1.178%). A set of 30 codons showed obvious codon usage bias, while 29 were A/U-ending codons. Six gene regions showed high levels of nucleotide diversity (Pi > 0.02). Totally, 273 SSRs were identified. Maximum-likelihood (ML) phylogenetic analysis revealed that R. molle was relatively closed to the R. pulchrum and R. delavayi. High similarity was detected among Ericaceae species, and the coding regions were more conserved than the non-coding regions. Expansion and contraction detected in IR region could be the main length variation in R. molle and related Ericaceae species. This research will supply rich genetic resource for R. molle and related species of Ericaceae.
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The associated BioProject, SRA, and Bio-Sample numbers are PRJNA765389, SRR16036101, and SAMN21557373, respectively.
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Acknowledgements
This work was supported by research grants from National Natural Science Foundation of China (NSFC 31500995), grant from Natural Science Foundation of Hubei Provincial Department of Science and Technology (2021CFB320), and grant from Scientific and Technological Research Project of Hubei Provincial Department of Education (D20222902) .
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Xu, B., Li, Z., Liu, Y. et al. Complete chloroplast genome sequence and phylogenetic analysis of Rhododendron molle G. Don, an endangered Ericaceae species located on Dabie Mountains (central China). Plant Biotechnol Rep 17, 303–314 (2023). https://doi.org/10.1007/s11816-023-00818-9
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DOI: https://doi.org/10.1007/s11816-023-00818-9