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The influence of sugar molecule type on the stability of lyophilized human serum albumin (HSA) nanocolloid kit

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Abstract

Using sugar molecules as lyophilization protectants in HSA nanocolloid kit formulations is critical to obtaining kits with excellent stability. A thermal gelation method with no sugar treatment and variations in sugar (glucose, maltose, and lactose) was applied to produce these kits. The particle size and radiochemical purity results showed that HSA nanocolloid kits with sugar excipients are more stable than those without sugar. The addition of lactose as an excipient (HSA: lactose 1:10) results in a stable nanocolloid system, allowing for the development of novel lyophilized HSA nanocolloid kit formulations.

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References

  1. International Atomic Energy Agency (2015) Radiopharmaceuticals for sentinel lymph node detection: status and trends iaea radioisotopes and radiopharmaceuticals series publications

  2. Marino G, Grassi T, Di Martino G et al (2022) Role of sentinel lymph node in endometrial cancer: rationale and surgical aspects, a review of the literature. Eur J Gynaecol Oncol 43:106. https://doi.org/10.31083/j.ejgo4301014

    Article  Google Scholar 

  3. Dogan NU, Dogan S, Favero G et al (2019) The basics of sentinel lymph node biopsy: anatomical and pathophysiological considerations and clinical aspects. J Oncol. https://doi.org/10.1155/2019/3415630

    Article  PubMed  PubMed Central  Google Scholar 

  4. Spada A, Emami J, Tuszynski JA, Lavasanifar A (2021) The uniqueness of albumin as a carrier in nanodrug delivery. Mol Pharm 18:1862–1894. https://doi.org/10.1021/acs.molpharmaceut.1c00046

    Article  CAS  PubMed  Google Scholar 

  5. Shen X, Liu X, Li T et al (2021) Recent advancements in serum albumin-based nanovehicles toward potential cancer diagnosis and therapy. Front Chem 9:1–19. https://doi.org/10.3389/fchem.2021.746646

    Article  CAS  Google Scholar 

  6. Kianfar E (2021) Protein nanoparticles in drug delivery: animal protein, plant proteins and protein cages, albumin nanoparticles. J Nanobiotechnol 19:1–32. https://doi.org/10.1186/s12951-021-00896-3

    Article  CAS  Google Scholar 

  7. Meng R, Zhu H, Wang Z et al (2022) Preparation of drug-loaded albumin nanoparticles and its application in cancer therapy. J Nanomater 2022:1–12. https://doi.org/10.1155/2022/3052175

    Article  CAS  Google Scholar 

  8. Hassanin I, Elzoghby A (2020) Albumin-based nanoparticles: a promising strategy to overcome cancer drug resistance. Cancer Drug Resist 3:930–946. https://doi.org/10.20517/cdr.2020.68

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Akbar MU, Ahmad MR, Shaheen A, Mushtaq S (2016) A review on evaluation of technetium-99m labeled radiopharmaceuticals. J Radioanal Nucl Chem 310:477–493. https://doi.org/10.1007/s10967-016-5019-7

    Article  CAS  Google Scholar 

  10. Ballinger JR (2022) Challenges in preparation of albumin nanoparticle-based radiopharmaceuticals. Molecules 27:1–11

    Article  Google Scholar 

  11. Wilhelm AJ, Mijnhout GS, Franssen EJF (1999) Radiopharmaceuticals in sentinel lymph-node detection—an overview. Eur J Nucl Med. https://doi.org/10.1007/s002590050576

    Article  PubMed  Google Scholar 

  12. He P, Tang H, Zheng Y et al (2023) Advances in nanomedicines for lymphatic imaging and therapy. J Nanobiotechnol 21:1–29. https://doi.org/10.1186/s12951-023-02022-x

    Article  CAS  Google Scholar 

  13. Abdelwahed W, Degobert G, Stainmesse S, Fessi H (2006) Freeze-drying of nanoparticles: formulation, process and storage considerations. Adv Drug Deliv Rev 58:1688–1713. https://doi.org/10.1016/j.addr.2006.09.017

    Article  CAS  PubMed  Google Scholar 

  14. Kaiser G. Sugar and its role in freeze-drying processes—investigated by means of DSC

  15. Chaudhari SP, Patil PS (2012) Pharmaceutical excipients: a review. Int J Adv Pharm Biol Chem 1:21–34

    Google Scholar 

  16. Balbani APS, Stelzer LB, Montovani JC (2006) Pharmaceutical excipients and the information on drug labels. Braz J Otorhinolaryngol 72:400–406. https://doi.org/10.1016/s1808-8694(15)30976-9

    Article  PubMed  Google Scholar 

  17. Hirsjärvi S, Peltonen L, Hirvonen J (2009) Effect of sugars, surfactant, and tangential flow filtration on the freeze-drying of poly(lactic acid) nanoparticles. AAPS PharmSciTech 10:488–494. https://doi.org/10.1208/s12249-009-9236-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Bedu-Addo FK (2004) Understanding lyophilization formulation development. Pharm Technol Lyophilization 20:10–18

    Google Scholar 

  19. Degobert G, Aydin D (2021) Lyophilization of nanocapsules: Instability sources, formulation and process parameters. Pharmaceutics. https://doi.org/10.3390/pharmaceutics13081112

    Article  PubMed  PubMed Central  Google Scholar 

  20. Trenkenschuh E, Friess W (2021) Freeze-drying of nanoparticles: How to overcome colloidal instability by formulation and process optimization. Eur J Pharm Biopharm 165:345–360. https://doi.org/10.1016/j.ejpb.2021.05.024

    Article  CAS  PubMed  Google Scholar 

  21. Marlina SE, Lestari E et al (2022) Surface modification of acid-functionalized mesoporous gamma-alumina for non-fission 99Mo/99mTc generator. Appl Radiat Isot 187:110342. https://doi.org/10.1016/j.apradiso.2022.110342

    Article  CAS  PubMed  Google Scholar 

  22. Wan KY, Weng J, Wong SN et al (2020) Converting nanosuspension into inhalable and redispersible nanoparticles by combined in-situ thermal gelation and spray drying. Eur J Pharm Biopharm 149:238–247. https://doi.org/10.1016/j.ejpb.2020.02.010

    Article  CAS  PubMed  Google Scholar 

  23. Lestari W, Setiyowati S, Triningsih R et al (2022) Optimasi sintesa nanokoloid human serum albumin sebagai agen limfoskintigrafi menggunakan central composite design-response surface methodology. J Kefarmasian Indones 12:69–78

    Article  Google Scholar 

  24. Egerton RF (2005) Physical principles of electron microscopy. An introduction to TEM, SEM, and AEM. Springer

    Book  Google Scholar 

  25. Malvern (2013) Zetasizer nano series user manual

  26. Kumar KN, Mallik S, Sarkar K (2017) Role of freeze-drying in the presence of mannitol on the echogenicity of echogenic liposomes. J Acoust Soc Am 142:3670–3676. https://doi.org/10.1121/1.5017607

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Ahmad T, Iqbal J, Bustam MA et al (2021) A critical review on phytosynthesis of gold nanoparticles: Issues, challenges and future perspectives. J Clean Prod 309:127460

    Article  CAS  Google Scholar 

  28. Malatesta M (2021) Transmission electron microscopy as a powerful tool to investigate the interaction of nanoparticles with subcellular structures. Int J Mol Sci. https://doi.org/10.3390/ijms222312789

    Article  PubMed  PubMed Central  Google Scholar 

  29. Danaei M, Dehghankhold M, Ataei S et al (2018) Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics 10:1–17. https://doi.org/10.3390/pharmaceutics10020057

    Article  CAS  Google Scholar 

  30. Takechi-Haraya Y, Ohgita T, Demizu Y et al (2022) Current status and challenges of analytical methods for evaluation of size and surface modification of nanoparticle-based drug formulations. AAPS PharmSciTech 23:1–20. https://doi.org/10.1208/s12249-022-02303-y

    Article  CAS  Google Scholar 

  31. Malvern (2011) Zeta potential: an introduction in 30 minutes

  32. Yu W, Xie H (2012) A review on nanofluids: preparation, stability mechanisms, and applications. J Nanomater 2012:1–17. https://doi.org/10.1155/2012/435873

    Article  Google Scholar 

  33. Honary S, Zahir F (2013) Effect of zeta potential on the properties of nano-drug delivery systems—a review (Part 1). Trop J Pharm Res 12:255–264. https://doi.org/10.4314/tjpr.v12i2.19

    Article  CAS  Google Scholar 

  34. Technical Reports Series no. 466 (2008) Technetium-99m radiopharmaceuticals: Manufactures of Kits. Austria

  35. Medi-Radiopharma (2007) Nano-albumon technical leaflet

  36. GE Healthcare (2015) NANOCOLL technical leaflet. Milan, Italy

  37. Varani M, Campagna G, Bentivoglio V et al (2021) Synthesis and biodistribution of 99mTc-labeled PLGA nanoparticles by microfluidic technique. Pharmaceutics. https://doi.org/10.3390/pharmaceutics13111769

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The present study was supported by The National Research and Innovation Agency (BRIN) under the Research and Innovation Program for Advanced Indonesia (decree number 82/II.7/HK/2022) and The Indonesian Endowment Funds for Education (LPDP). The authors are grateful to Ms. Grace Tjungirai Sulungbudi and Ms. Mujamilah (Research Center for Radiation Detection and Nuclear Analysis Technology) for their continued support of this project.

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Authors and Affiliations

  1. Research Center for Radioisotopes, Radiopharmaceuticals, and Biodosimetry Technology, Research Organization for Nuclear Energy - National Research and Innovation Agency (BRIN), KST BJ Habibie, Building Number 11 Puspiptek Area, South Tangerang, 15314, Indonesia

    Ratna Dini Haryuni, Wening Lestari, Sumandi Juliyanto, Veronika Yulianti Susilo, Amal Rezka Putra, Ahsanal Fikri, Ligwina Dita Pertiwi, Sri Setiyowati &  Triningsih

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  1. Ratna Dini Haryuni
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Contributions

All authors contributed equally to this work. RDH: designed the study, wrote the manuscript, conducted experiments, supervised the work, WL: methodology, performed experiments, funding acquisition, project administration, SJ: the main conceptual ideas, editing, analyzed the data, conducted the experiments, VYS: performed experiments, funding acquisition, ARP: visualization, data curation, performed experiments, AF: visualization, data curation, performed experiments, LDP: processed the experimental data, performed the analysis, conducted experiments, SS: methodology, performed experiments, T: performed the analysis, conducted experiments.

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Correspondence to Ratna Dini Haryuni.

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Haryuni, R.D., Lestari, W., Juliyanto, S. et al. The influence of sugar molecule type on the stability of lyophilized human serum albumin (HSA) nanocolloid kit. J Radioanal Nucl Chem 333, 1315–1322 (2024). https://doi.org/10.1007/s10967-024-09384-y

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