WO2016038067A1 - Use of afidopyropene in genetically modified plants - Google Patents

Abstract

The present invention relates to agricultural methods for controlling pests and/or increasing the plant health of cultivated plants having at least one modification, by treating such cultivated plants, parts of such plants, plant propagation materials or their locus of growth with afidopyropen or compositions comprising afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient.

Description

USE OF AFIDOPYROPENE IN GENETICALLY MODIFIED PLANTS

The present invention relates to a method for controlling pests and/or increasing the plant health of a cultivated plant with at least one (genetic) modification (hereinafter abbreviated as "cultivat- ed plant" and described in more details further below) as compared to the respective non- modified control plant, comprising the application of a pesticidally active pyripyropene compound, especially afidopyropen, to a cultivated plant, parts of such plant, plant propagation material, or at its locus of growth. Cultivated plants may be for example plants that have - optionally also among others - an insecticidal trait. It is a wide-spread problem that insects, that are combatted with insectides, develop resistance, i.e. they become less or not all anymore susceptible to the insecticidal effect.

It has surprisingly turned out, that afidopyropene or mixtures or compositions comprising afidopyropene are useful in methods of controlling harmful insects by treating cultivated plants, parts of such plants or their locus of growth, wherein the plant has at least one trait. For example methods of controlling harmful insects by treating cultivated plants, parts of such plants or their locus of growth, wherein the plant has at least one insecticidal trait and wherein the harmful insects are resistant to an insecticidal trait of the plant.

Pesticides are known to be harmful to humans and to animals in general. The environmental consequences that these pesticides cause is often complicated due to the methods of applying the pesticides by foliar spraying or direct application to the surface of the soil.

Wind-drift, leaching, and runoff can cause the migration and spreading of some percentages of the pesticide out of the field of applicationdesired zone of activity.

Because of such concern efforts have been made in the past to find ways to reduce the amount of chemical pesticides that are used. For example some of these efforts have focused on the development of transgenic crops that are engineered.

Nevertheless, a transgenic event alone may not be sufficiently effective to protect crops from damage by pests. The total control of pest damage by any one transgenic event may even not be desirable in the long term, because of the potential for the development of resistant strains of the target pest.

Alternatives to the conventional forms of pesticide application according to the present invention is the treatment of plant proparagation material, preferably seeds, with pesticides. The use of pesticides to protect seeds from attack after planting, and the use of low levels of insecticides for the protection of the crop has been used for some time. Seed treatment with pesticides has the advantages providing for the protection of the seeds, while minimizing the amount of pesticide that is required and limiting the amount of contact with the pesticide and the number of different field applications that are necessary.

Therefore, although recent developments in genetic engineering of plants have improved the ability to protect plants from pests without using chemical pesticides, and while have reducing the harmful effects of pesticides on the environment, numerous problems remain that limit the successful application of these methods under actual field conditions. Accordingly, it would be useful to provide an improved method for the protection of plants, especially cultivated plants, from damage by pests.

It would also be particularly useful if such method would reduce the required application rate of the pesticides, and also if it would limit the number of separate field operations that were required for crop planting and cultivation.

Thus the present invention is directed to a novel method for protecting a cultivated plant against damage by one or more pests, the method comprising providing a pesticide for cultivated plant or a treated seed which seed comprises a transgenic event. Among the several advantages found to be achieved by the present invention, therefore, may be noted the provision of an improved method for the protection of cultivated plants with afidopyropen from damage by pests; the provision of such a method that would reduce the required application rate of conventional chemical pesticides; and also the provision of such a method that would limit the number of separate field operations that were required for crop planting and cultivation.

Although afidopyropen and the naturally occurring insecticidal pyripyropene A derivative themselves and their combined application with other insecticides are known to have shown activity against certain crop damaging insect pests, afidopyropen and some of its selected mixtures with pesticidally active compounds (II) have not yet been described for solving discussed problems as mentioned above.

The pyripyropene pesticide afidopyropen of the following formula (I), which in the following may also be referred to as "compound I", "compound of formula I" or the "compound of the present invention",

is known from WO 2009/081851 (Examples, compound 4) and belongs to the class of pyripyropene derivatives.

WO 2009/081851 discloses various agrochemical formulations of afidopyropen and useful additives for agrochemical formulations of it. EP 1 889 540 and EP 2223599 disclose various agro- chemical formulations of pyripyropene derivatives. The Afidopyropen may be prepared by the process described in WO 2006/129714, EP 2 186 815, EP2426124, EP1889540 and

EP2426124. Further, EP 2 1 19 361 , WO201 1 147952 and WO201 1 147853 describe different mixtures of afidopyropen with other insecticidal or fungicidal active ingrdients. Pyripyropene A (pyripyropene pesticide of formula I .A herein below), produced e.g. by the method described in Journal of Society of Synthetic Organic Chemistry, Japan (1998), Vol. 56, No. 6, pp. 478-488 or WO 94/09417, may for example be used as starting material for preparing further pyripyropene derivatives.

(Formula I. A)

Pyripyropene A has inhibitory activity against ACAT (acyl-CoA: cholesterol acyltransferase) and is expected to be applied, for example, for the treatment of diseases induced by cholesterol ac- cumulation, as described in Japanese Patent No. 2993767 (Japanese Patent Laid-Open Publication No. 360895/1992 ) and Journal of Antibiotics (1993), 46(7), 1 168-9.

Furthermore, Applied and Environmental Microbiology (1995), 61 (12), 4429-35 describes that pyripyropene A itself has insecticidal activity against larvae of Helicoverpa zea. Furthermore, WO 2004/060065 describes that pyripyropene A has insecticidal activity against Plutella xy- lostella L larvae and Tenebrio molitor L.

The subject matter of the present invention includes methods for protecting cultivated plants from attack or infestation by insects, acarids or nematodes comprising contacting the plant, or the soil or water in which the plant is growing, with afidopyropen, especially with a composition comprising afidopyropen in pesticidally effective amounts; a method for controlling insects, arachnids or nematodes comprising contacting an insect, acarid or nematode or their food supply, habitat, breeding grounds or their locus with afidopyropen, especially with a composition comprising afidopyropen in pesticidally effective amounts; a method for protection of plant propagation material comprising contacting the cultivated plant propagation material, preferably seeds, with afidopyropen, especially with a composition comprising afidopyropen in pesticidally effective amounts; and finally seeds as such cultivated plant propagation material, comprising said composition.

The methods and mixtures described herein may also be applied and administrated with pyri- pyropene A as an insecticidal active pyripyropene derivative. Thus in the following it may also be referred to as "compounds of the present invention". However, preference is given in each and every case to afidopyropen as pesticidal active ingredient.

The term "composition(s) according to the invention" or "composition(s) of the present invention" encompasses composition(s) comprising at least one compound of formula (I) or mixtures of the compounds of formula (I) with other pesticidally active compound(s) II for being used and/or applied in methods according to the invention as defined above.

The pyripyropene derivative afidopyropen applied in methods and uses according to the present invention comprise the compound as defined herein as well as a known stereoisomer, salt, tautomer or N-oxide thereof (including a polymorphic crystalline form, a co-crystal or a solvate of a compound or a stereoisomer, salt, tautomer or N-oxide thereof. Same would apply to the pyripyropene A.

Pests

In the methods according to the invention, afidopyropen and mixtures comprising afidopyropene are in particular suitable for efficiently controlling arthropodal pests such as arachnids, myriape- des and insects as well as nematodes. With regard to the present invention, the term pests embrace animal pests (such as insects, acarids or nematodes). The term animal pests include, but are not limited to the following genera and species: insects from the order of Lepidoptera, for example Achroia grisella, Aden^'s spp. such as A. fimbriana, A. gloverana, A. variana; Acrolepiopsis assectella, Acronicta major, Adoxophyes spp. such as A. cyrtosema, A. orana; Aedia leucomelas, Agrotis spp. such as A. exclamationis, A. fucosa, A. ipsiion, A. orthogoma, A. segetum, A. subterranea; Alabama argiiiacea, Aleurodicus dispersus, Alsophila pometaria, Ampelophaga rubiginosa, Amyelois transitella, Anacampsis sarcitella, Anagasta kuehniella, Anarsia lineatella, Anisota senator/a, Antheraea pernyi, Anticar- sia (=Thermesia) spp. such as A. gemmatalis; Apamea spp., Aproaerema modicella, Archips spp. such as A. argyrospila, A. fuscocupreanus, A. rosana, A. xyloseanus; Argyresthia conjugel- la, Argyroploce spp., Argyrotaenia spp. such as A. velutinana; Athetis mindara, Austroasca vi- ridigrisea, Autographa gamma, Autographa nigrisigna, Barathra brassicae, Bedel/la spp., Bon- agota salubricola, Borbo cinnara, Bucculatrix thurberiella, Bupalus piniarius, Busseola spp., Cacoecia spp. such as C. murinana, C. podana; Cactoblastis cactorum, Cadra cautella, Calingo braziliensis, Caloptilis theivora, Capua reticulana, Carposina spp. such as C. niponensis, C. sasakii; Cephus spp., Chaetocnema aridula, Cheimatobia brumata, Chilo spp. such as C. Indi- cus, C. suppressalis, C. partellus; Choreutis pariana, Choristoneura spp. such as C. conflictana, C. fumiferana, C. longicellana, C. murinana, C. occidentalis, C. rosaceana; Chrysodeixis (=Pseudoplusia) spp. such as C. eriosoma, C. includens; Cirphis unipuncta, Clysia ambiguella, Cnaphalocerus spp., Cnaphalocrocis medinalis, Cnephasia spp., Cochylis hospes, Coleophora spp., Colias eurytheme, Conopomorpha spp., Conotrachelus spp., Cop/tarsia spp., Corcyra cephalonica, Crambus caliginosellus, Crambus teterrellus, Crocidosema (=Epinotia) aporema, Cydalima (=Diaphania) perspectalis, Cydia (=Carpocapsa) spp. such as C. pomonella, C. latiferreana; Dalaca noctuides, Datana integerrima, Dasychira pinicola, Dendrolimus spp. such as D. pini, D. spectabilis, D. sibiricus; Desmia funeralis, Diaphania spp. such as D. nitidalis, D. hyalinata; Diatraea grandiosella, Diatraea saccharalis, Diphthera festiva, Earias spp. such as E. insulana, E. vittella; Ecdytolopha aurantianu, Egira (=Xylomyges) curia/is, E/asmopa/pus ligno- sellus, Eldana saccharina, Endopiza viteana, Ennomos subsignaria, Eoreuma loftini, Ephestia spp. such as E. cautella, E. elutella, E. kuehniella; Epinotia aporema, Epiphyas postvittana, Erannis tiliaria, Erionota thrax, Etiella spp., Eulia spp., Eupoecilia ambiguella, Euproctis chrysorrhoea, Euxoa spp., Evetria bouliana, Faronta albilinea, Fe/tia spp. such as F. subterranean; Galleria mellonella, Grac/i/aria spp., Grapholita spp. such as G. funebrana, G. molesta, G inopinata; Halysidota spp., Harrisina am erica na, Hedylepta spp., Helicoverpa spp. such as H. armigera (=Heliothis armigera), H. zea (=Heliothis zea); Heliothis spp. such as H. assulta, H. subfiexa, H. virescens; Heiiuia spp. such as H. undalis, H. rogataiis; Helocoverpa geiotopoeon, Hemileuca oliviae, Herpetogramma licarsisalis, Hibernia defoliaria, Hofmannophila pseu- dospretella, Homoeosoma electellum, Homona magnanima, Hypena scabra, Hyphantria cunea, Hyponomeuta pa del la, Hyponomeuta ma Im^' el I us, Kakivoria flavofasciata, Keiferia lycopersicella, Lambdina fiscellaria fiscellaria, Lambdina fiscellaria lugubrosa, Lamprosema indicata, Laspeyresia molesta, Leguminivora glycinivorella, Lerodea eufala, Leucinodes orbonalis, Leu- coma salicis, Leucoptera spp. such as L coffee/la, L. scitella; Leuminivora lycinivorella, Lithocolletis blancardella, Lithophane antennata, Llattia octo (=Amyna axis), Lobesia botrana, Lophocampa spp., Loxagrotis albicosta, Loxostege spp. such as L. sticticalis, L. cereralis; Lymantria spp. such as L. dispar, L. monacha; Lyonetia clerkella, Lyonetia prunifoliella, Malaco- soma spp. such as M. americanum, M. californicum, M. constrictum, M. neustria; Mamestra spp. such as M. brassicae, M. configurata; Mamstra brassicae, Manduca spp. such as M. quin- quemaculata, M. sexta; Marasmia spp, Marmara spp., Maruca test u la I is, Megalopyge lanata, Melanchra picta, Melanitis leda, Mods spp. such as M. lapites, M. repanda; Mods latipes, Mon- ochroa fragariae, Mythimna separata, Nemapogon cioaceiia, Neoleucinodes elegantalis, Nepytia spp., Nymphula spp., Oiketicus spp., Omiodes indicata, Omphisa anastomosalis, Oper- ophtera brumata, Orgyia pseudotsugata, Oria spp., Orthaga thyrisalis, Ostrinia spp. such as O. nubilalis; Oulema oryzae, Paleacrita vernata, Pan oils flammea, Parnara spp., Papaipema nebris, Papilio cresphontes, Paramyelois transitella, Paranthrene regalis, Paysandisia archon, Pectinophora spp. such as P. gossypiella; Peridroma saucia, Perileucoptera spp., such as P. coffeella; Phalera bucephala, Phryganidia calif ornica, Phthorimaea spp. such as P. operculella; Phyllocnistis citrella, Phyllonorycter spp. such as P. blancardella, P. crataegella, P. issikii, P. ringoniella; Pieris spp. such as P. brassicae, P. rapae, P. napi; Pilocrocis tripunctata, Plathy- pena scabra, Platynota spp. such as P. flavedana, P. idaeusalis, P. stultana; Platyptilia cardui- dactyla, Plebejus argus, Plodia interpunctella, Plusia spp, Plutella maculipennis, Plutella xy- lostella, Pontia protodica, Prays spp., Prodenia spp., Proxenus /epigone, Pseuda/et/a spp. such as P. sequax, P. unipuncta; Pyrausta nubilalis, Rachi plusia nu, Rich la albicosta, Rhizobius ven- tralis, Rhyacionia frustrana, Sabu lodes a eg rota ta, Schizura concinna, Schoenobius spp., Schreckensteinia festaliella, Scirpophaga spp. such as S. incertulas, S. innotata; Scotia segetum, Sesamia spp. such as S. inferens, Seudyra subflava, Sitotroga cerealella, Spargan- oth/s pilleriana, Spilonota lechriaspis, S. ocellana, Spodoptera (=Lamphygma) spp. such as S. cosmoides, S. eridania, S. exigua, S. frugiperda, S. latisfascia, S. littoralis, S. litura, S. omitho- galli; Stigmella spp., Stomopteryx subsecivella, Strymon bazochii, Sylepta derogata, Synanthe- don spp. such as S. exitiosa, Tec/a so/an/vora, Te/eh/n licus, Thaumatopoea pityocampa, Thaumatot/bia (=Cryptophlebia) leucotreta, Thaumetopoea pityocampa, Thecla spp., Theresi- mima ampelophaga, Thyrinteina spp, Tildenia inconspicueiia, Tinea spp. such as T. cioaceiia, T. pellionella; Tineola bisselliella, Tortrix spp. such as T viridana; Trichophaga tapetzella, Tri- choplusia spp. such as T ni; Tuta (=Scrobipalpula) absoluta, Udea spp. such as U. rubigalis, U. rubigalis; Virachola spp., Yponomeuta padella, and Zeiraphera canadensis; insects from the order of Coleoptera, for example Acalymma vittatum, Acanthoscehdes obtec- tus, Adoretus spp., Agelastica alni, Agrilus spp. such as A. anxius, A. planipennis, A. sinuatus; Agriotes spp. such as A. fuscicollis, A. lineatus, A. obscurus; Alphitobius diaperinus, Amphimal- lus solstitialis, Anisandrus dispar, Anisoplia austriaca, Anobium punctatum, Anomala corpulen- ta, Anomala rufocuprea, Anoplophora spp. such as A. glabripennis; Anthonomus spp. such as A. eugenii, A. grandis, A. pomorum; Anthrenus spp., Aphthona euphoridae, Apion spp., Apogo- nia spp., Athous haemorrhoidalis, Atomaria spp. such as A. linearis; Attagenus spp., Aula- cophora femora/is, Blastophagus piniperda, Blitophaga undata, Bruchidius obtectus, Bruchus spp. such as z?. lentis, B. pisorum, B. rufimanus; Byctiscus be tula e, Callidiellum rufipenne, Cal- lop/stria floridensis, Callosobruchus chinensis, Cameraria ohridella, Cassida nebulosa, Ceroto- ma trifurcata, Cetonia aurata, Ceuthorhynchus spp. such as C. assimilis, C. napi; Chaetocnema tibialis, Cleonus mendicus, Conoderus spp. such as C. vespertinus; Conotrachelus nenuphar, Cosmopolites spp., Costelytra zealandica, Crioceris asparagi, Cryptolestes ferrugineus, Cryp- torhynchus lapathi, Ctenicera spp. such as C. destructor; Curculio spp., Cylindrocopturus spp., Cyclocephala spp., Dactylispa balyi, Dectes texanus, Dermestes spp., Diabrotica spp. such as D. undecimpunctata, D. speciosa, D. longicornis, D. semipunctata, D. virgifera; Diaprepes abbreviates, Dichocrocis spp., Dicladispa armigera, Diloboderus abderus, Diocalandra frumenti (Diocalandra stigmaticollis), Enaphalodes rufulus, Epilachna spp. such as E. varivestis, E. vigintioctomaculata; Epitrix spp. such as E. hirtipennis, E. similaris; Eutheola humilis, Eu- tinobothrus brasiliensis, Fa us tin us cubae, Gibbium psylloides, Gnathocerus cornutus, Hellula undalis, Heteronychus arator, Hylamorpha elegans, Hylobius abietis, Hylotrupes bajulus, Hy- pera spp. such as H. brunneipennis, H. postica; Hypomeces squamosus, Hypothenemus spp., Ips typographus, Lachnosterna consanguinea, Lasioderma serricorne, Latheticus oryzae, Lath- ridius spp., Lema spp. such as L. bilineata, L. melanopus; Leptinotarsa spp. such as L. decem- lineata; Leptispa pygmaea, Limonius californicus, Lissorhoptrus oryzophilus, Lixus spp., Lu- perodes spp., Lyctus spp. such as L. bruneus; Liogenys fuscus, Macrodactylus spp. such as M. subspinosus; Maladera matrida, Megaplatypus mutates, Megascelis spp., Melanotus communis, Meligethes spp. such as M. aeneus; Melolontha spp. such as M. hippocastani, M. melol- ontha; Metamasius hemipterus, Microtheca spp., Migdolus spp. such as M. fryanus, Monocha- mus spp. such as M. alternatus; Naupactus xanthographus, Niptus hololeucus, Oberia brevis, Oemona hirta, Oryctes rhinoceros, Oryzaephilus surinamensis, Oryzaphagus oryzae, Otiorrhyn- chus sulcatus, Otiorrhynchus ovatus, Otiorrhynchus sulcatus, Oulema melanopus, Oulema oryzae, Oxycetonia jucunda, Phaedon spp. such as P. brassicae, P. cochleariae; Phoracantha re- curva, Phyllobius pyri, Phyllopertha horticola, Phyllophaga spp. such as P. helleri; Phyllotreta spp. such as P. chrysocephala, P. nemorum, P. striolata, P. vittula; Phyllopertha horticola, Pop- illia japonica, Premnotrypes spp., Psacothea hilaris, Psylliodes chrysocephala, Prostephanus truncates, Psylliodes spp., Ptinus spp., Pulga saltona, Rhizopertha dominica, Rhynchophorus spp. such as R. biiiineatus, R. ferrugineus, R. paimarum, R. phoenicis, R. vuineratus; Saperda Candida, Scolytus schevyrewi, Scyphophorus acupunctatus, Sitona lineatus, Sitophilus spp. such as S. granaria, S. oryzae, S. zeamais; Sphenophorus spp. such as S. levis; Stegobium paniceum, Sternechus spp. such as S. subsignatus; Strophomorphus ctenotus, Symphyletes spp., Tanymecus spp., Tenebrio molitor, Tenebrioides mauretanicus, Tribolium spp. such as T. castaneum; Trogoderma spp., Tychius spp., Xylotrechus spp. such as X. pyrrhoderus; and, Za- brus spp. such as Z. tenebrioides; insects from the order of Diptera for example Aedes spp. such as A aegypti, A. albopictus, A. vexans; Anastrepha ludens, Anopheles spp. such as A. albimanus, A. crucians, A. freeborn/, A. gambiae, A. leucosphyrus, A. maculipennis, A. minimus, A. quadrimaculatus, A. sinensis; Bac- trocera invadens, Bibio hortulanus, Calliphora erythrocephala, Calliphora vicina, Ceratitis capi- tata, Chrysomyia spp. such as C. bezziana, C. hominivorax, C. macellaria; Chrysops atlanticus, Chrysops discalis, Chrysops silacea, Cochliomyia spp. such as C. hominivorax; Contarinia spp. such as C. sorghicola; Cordylobia anthropophaga, Culex spp. such as C. nigripalpus, C. pip/ens, C. quinquefasciatus, C. tarsalis, C. tritaeniorhynchus; Culicoides furens, Culiseta inor- nata, Culiseta melanura, Cuterebra spp., Dacus cucurbitae, Dacus oleae, Dasineura brassicae, Dasineura oxycoccana, Delia spp. such as D. antique, D. coarctata, D. platura, D. radicum; Dermatobia hominis, Drosophila spp. such as D. suzukii, Fannia spp. such as F. canicularis; Gastraphilus spp. such as G. in test/nails; Geomyza t/punctata, G/ossina spp. such as G fusci- pes, G morsitans, G paipaiis, G tachinoides; Haematobia irritans, Haplodiplosis equestris, Hippelates spp., Hylemyia spp. such as H. platura; Hypoderma spp. such as H. lineata; Hyppo- bosca spp., Hydrellia philippina, Leptoconops torrens, Liriomyza spp. such as L. sativae, L. trifo- lii; Lucilia spp. such as L. caprina, L. cuprina, L. sericata; Lycoria pectoralis, Mansonia titillanus, Mayetiola spp. such as M. destructor; Musca spp. such as M. autumnalis, M. domestica; Musci- na stabuians, Oestrus spp. such as O ovis; Opomyza fiorum, Oscineiia spp. such as O frit; Orseolia oryzae, Pegomya hysocyami, Phlebotomus argentipes, Phorbia spp. such as P. anti- qua, P. brassicae, P. coarctata; Phytomyza gymnostoma, Prosimulium mixtum, Psila rosae, Psorophora columbiae, Psorophora discolor, Rhagoletis spp. such as R. cerasi, R. cingulate, R. indifferens, R. mendax, R. pomonella; Rivellia quadrifasciata, Sarcophaga spp. such as S. haemorrhoidalis; Sim u Hum vittatum, Sitodiplosis mosellana, Stomoxys spp. such as S. calci- trans; Tabanus spp. such as T. atratus, T. bovinus, T. lineola, T. similis; Tannia spp., Thecodi- ptosis japonensis, Tipula oleracea, Tipula paludosa, and Wohlfahrtia spp;

insects from the order of Thysanoptera for example, Baliothrips biformis, Dichromothrips cor- betti, Dichromothrips ssp., Echinothrips americanus, Enneothrips flavens, Frankliniella spp. such as F. fusca, F. occidentalis, F. tritici; Heliothrips spp., Hercinothrips femora/is, Kakothrips spp., Microcephalothrips abdominalis, Neohydatothrips samayunkur, Pezothrips kellyanus, Rhipiphorothrips cruentatus, Scirtothrips spp. such as S. citri, S. dorsalis, S. perseae; Stenchae- tothrips spp, Taeniothrips cardamoni, Taeniothrips inconsequens, Thrips spp. such as T. imagines, T. hawaiiensis, T. oryzae, T. pa I mi, T. parvispinus, T. tabaci;

insects from the order of Hemiptera for example, Acizzia jamatonica, Acrosternum spp. such as A. hilare; Acyrthosipon spp. such as A. onobrychis, A. pisum; Adelges laricis, Adelges tsu- gae, Adelphocoris spp., such as A. rapidus, A. superbus; Aeneolamia spp., Agonoscena spp., Aulacorthum solani, Aleurocanthus woglumi, Aleurodes spp., Aleurodicus disperses, Aleurolo- bus barodensis, Aleurothrixus spp., Amrasca spp., Anasa tristis, Antestiopsis spp., Anuraphis cardui, Aonidiella spp., Aphanostigma piri, Aphidula nasturtii, Aphis spp. such as A. craccivora, A. fabae, A. forbesi, A. gossypii, A. grossulariae, A. maidiradicis, A. pomi, A. sambuci, A. schneideri, A. spiraeco/a; Arbor/d/a apica/is, Ar/ius cr/tatus, Aspidiella spp., Aspidiotus spp., Atanus spp., Aulacaspis yasumatsui, Aulacorthum solani, Bactericera cockerelli (Paratrioza cockerelli), Bemisia spp. such as B. argentifolii, B. tabaci (Aleurodes tabaci); Blissus spp. such as B. leucopterus; Brachycaudus spp. such as B. cardui, B. heiichrysi, B. persicae, B. prunicoia; Brachyco/us spp., Brachycorynella asparagi, Brevicoryne brassicae, Cacopsylla spp. such as C fulguralis, C. pyricola (Psylla piri); Calligypona marginata, Calocoris spp., Campylomma livida, Capitophorus horn/, Carneocephala fulgida, Cavelerius spp., Ceraplastes spp., Ceratovacuna lanigera, Ceroplastes ceriferus, Cerosipha gossypii, Chaetosiphon fragaefolii, Chionaspis te- galensis, Chlorita onukii, Chromaphis juglandicola, Chrysomphalus ficus, Cicadulina mbila, Ci- mex spp. such as C. hemipterus, C. lectularius; Coccomytilus ha Hi, Coccus spp. such as C. hesperidum, C. pseudomagnoliarunr, Corythucha arcuata, Creontiades diiutus, Cryptomyzus ribis, Chrysomphalus aonidum, Cryptomyzus ribis, Ctenarytaina spatulata, Cyrtopeltis notatus, Dalbulus spp., Dasynus piperis, Dialeurodes spp. such as D. citrifolii; Dalbulus maidis, Di- aphorina spp. such as D. citri; D/asp/s spp. such as D. bromeliae; Dichelops furcatus, Diconoco- ris hewetti, Dora/is spp., Dreyfus/a nordmannianae, Dreyfus/a piceae, Drosicha spp., Dysaphis spp. such as D. piantaginea, D. pyri, D. radicola; Dysaulacorthum pseudosolani, Dysdercus spp. such as D. cingulatus, D. intermedius; Dysmicoccus spp., Edessa spp., Geocoris spp., Empoasca spp. such as E. fabae, E. so/ana; Ep/diaspis leperii, Eriosoma spp. such as E. lanig- erum, E. pyricola; Erythroneura spp., Eurygaster spp. such as E. integriceps; Euscelis bilobatus, Euschistus spp. such as E. heros, E. impictiventris, E. servus; Fiorinia theae, Geococcus coffe- ae, Glycaspis brimblecombei, Halyomorpha spp. such as H. halys; Heliopeltis spp., Homalodis- ca vitripennis (=H. coagulata), Horcias nobilellus, Hyalopterus pruni, Hyperomyzus lactucae, lcerya spp. such as /. purchase; Idiocerus spp., Idioscopus spp., Laodelphax striatellus, Lecani- um spp., Lecanoideus floccissimus, Lepidosaphes spp. such as L. ulmi; Leptocorisa spp., Lep- toglossus phyllopus, Lipaphis erysimi, Lygus spp. such as L. hesperus, L. lineolaris, L. praten- sis; Maconellicoccus hirsutus, Marchalina h el/en lea, Macro pes excavatus, Macrosiphum spp. such as M. rosae, M. avenae, M. euphorbiae; Macrosteles quadrilineatus, Mahanarva fimbriola- ta, Megacopta cribraria, Megoura viciae, Melanaphis pyrarius, Melanaphis sacchari, Melanocal- lis (=Tinocallis) caryaefoliae, Metcafiella spp., Metopolophium dirhodum, Monellia costalis, Mo- nelliopsis pecanis, Myzocallis coryli, Murgantia spp., Myzus spp. such as M. ascalonicus, M. cerasi, M. nicotianae, M. persicae, M. varians; Nasonovia ribis-nigri, Neotoxoptera formosana, Neomegalotomus spp, Nephotettix spp. such as N. malayanus, N. nigropictus, N. parvus, N. virescens; Nezara spp. such as N. viridula; Nilaparvata lugens, Nysius huttoni, Oebalus spp. such as O. pugnax; Oncometopia spp., Orthezia praelonga, Oxycaraenus hyalinipennis, Para- bemisia myricae, Parlatoria spp., Parthenolecanium spp. such as P. corn/, P. persicae; Pemphigus spp. such as P. bursarius, P. popuiivenae; Peregrinus maidis, Perkinsieiia saccharicida, Phenacoccus spp. such as P. aceris, P. gossypii; Phloeomyzus passerinii, Phorodon humuli, Phylloxera spp. such as P. devastatrix, Piesma quadrata, Piezodorus spp. such as P. guildinii; Pinnaspis aspidistrae, Planococcus spp. such as P. citri, P. ficus; Prosapia bicincta, Protopulvi- naria pyri for mis, Psallus seriatus, Pseudacysta perse a, Pseudaulacaspis pentagona, Pseudo- coccus spp. such as P. comstocki; Psylla spp. such as P. mali; Pteromalus spp., Pulvinaria amygdali, Pyrilla spp., Quadraspidiotus spp., such as Q. perniciosus; Quesada gigas, Rastro- coccus spp., Reduvius senilis, Rhizoecus americanus, Rhodnius spp., Rhopalomyzus ascalonicus, Rhopalosiphum spp. such as R. pseudobrassicas, R. insertum, R. maidis, R. pad/; Saga- todes spp., Sahlbergella singularis, Saissetia spp., Sappaphis mala, Sappaphis mali, Scapto- coris spp., Scaphoides titan us, Schizaphis gram in um, Schizoneura lanuginosa, Scotinophora spp., Selenaspidus articulatus, Sitobion avenae, Sogata spp., Sogatella furcifera, Solubea insu- laris, SpissistHus festinus (=Stictocephala festina), Stephanitis nashi, Stephanitis pyrioides, Stephanitis takeyai, Tenalaphara malayensis, Tetraleurodes perseae, Therioaphis maculate, Thyanta spp. such as T. accerra, T. perditor; Tibraca spp., Tomaspis spp., Toxoptera spp. such as T. aurantii; Trialeurodes spp. such as T. abutilonea, T. ricini, T. vaporariorum; Triatoma spp., Trioza spp., Typh/ocyba spp., Unaspis spp. such as U. citri, U. yanonensis; and Viteus vitifolii, Insects from the order Hymenoptera for example Acanthomyops interjectus, Athalia rosae, At- ta spp. such as A. capiguara, A. cephalotes, A. cephalotes, A. laevigata, A. robusta, A. sexdens, A. texana, Bombus spp., Brachymyrmex spp., Camponotus spp. such as C. florida- nus, C. pennsylvanicus, C. modoc; Cardiocondyla nuda, Chalibion sp, Crematogaster spp., Dasymutilla occidentalis, Diprion spp., Dolichovespula maculata, Dorymyrmex spp., Dryocos- mus kuriphilus, Formica spp., Hoplocampa spp. such as H. minuta, H. testudinea; Iridomyrmex humilis, Lasius spp. such as L. niger, Linepithema humile, Liometopum spp., Leptocybe invasa, Monomorium spp. such as M. pharaonis, Monomorium, Nylandria fulva, Pachycondyla chinen- sis, Paratrechina longicornis, Paravespula spp., such as P. germanica, P. pennsylvanica, P. vulgaris; Pheidole spp. such as P. megacephala; Pogonomyrmex spp. such as P. barbatus, P. californicus, Polistes rubiginosa, Prenolepis impairs, Pseudomyrmex gracilis, Schelipron spp., Sirex cyaneus, Solenopsis spp. such as S. geminata, S.invicta, S. molesta, S. richteri, S. xyloni, Sphecius speciosus, Sphex spp., Tapinoma spp. such as T. melanocephalum, T. sessile; Tetramorium spp. such as T. caespitum, T. bicarinatum, Vespa spp. such as V. crabro; Vespula spp. such as V. squamosal; Wasmannia auropunctata, Xylocopa sp;

Insects from the order Orthoptera for example Acheta domesticus, Calliptamus italicus, Chor- toicetes terminifera, Ceuthophilus spp., Diastrammena asynamora, Dociostaurus maroccanus, Gryllotalpa spp. such as G. africana, G. gryllotalpa; Gryllus spp., Hieroglyphus daganensis, Kraussaria anguiifera, Locusta spp. such as L. migratoria, L. pardalina; Meianopius spp. such as M bivittatus, M. femurrubrum, M. mexicanus, M. sanguinipes, M. spretus; Nomadacris sep- temfasciata, Oedaleus senegalensis, Scapteriscus spp., Schistocerca spp. such as S. america- na, S. gregaria, Stemopelmatus spp., Tachycines asynamorus, and Zonozerus variegatus;

Pests from the Class Arachnida for example Acari,e.g. of the families Argasidae, Ixodidae and Sarcoptidae, such as Amblyomma spp. (e.g. A. americanum, A. variegatum, A. macuiatum), Argas spp. such as A. persicu), Boophilus spp. such as B. annulatus, B. decoloratus, B. mi- croplus, Dermacentor spp. such as D.silvarum, D. andersoni, D. variabilis, Hyalomma spp. such as H. truncatum, Ixodes spp. such as /. ricinus, I. rubicundus, I. scapularis, I. holocyclus, I. pacificus, Rhipicephalus sanguineus, Ornithodorus spp. such as O. moubata, O. hermsi, O. turicata, Ornithonyssus bacoti, Otobius megnini, Dermanyssus gallinae, Psoroptes spp. such as P. ovis, Rhipicephalus spp. such as R. sanguineus, R. appendiculatus, Rhipicephalus everts/, Rh/zog/yphus spp., Sarcoptes spp. such asS. Scabiei, and Family Eriophyidae including Acer/a spp. such as A. she/doni, A. anthocoptes, A callitus spp., Aculops spp. such as A. lycopersici, A. pe/ekassi, Acu/us spp. such as A. sch/echtenda/i; Colomerus vitis, Epitrimerus pyri, Phyllo- coptruta oleivora; Eriophytes ribis and Eriophyes spp. such as Eriophyes she/doni, Family Tar- sonemidae including Hemitarsonemus spp., Phytonemus pallidus and Polyphagotarsonemus latus, Stenotarsonemus spp. Steneotarsonemus spinki, Family Tenuipalpidae including Brevi- palpus spp. such as B. phoen/cis, Family Tetranychidae including Eotetranychus spp., Eute- tranychus spp., Oiigonychus spp., Petrobia iatens, Tetranychus spp. such as T. cinnabarinus, T. evansi, T. kanzawai, T, pacificus, T. phaseulus, T. telarius and T. urticae, Bryobia praetiosar, Panonychus spp. such as P. uimi, P. citri, Metatetranychus spp. and Oiigonychus spp. such as O. pratensis, O. perseae, Vasates lycopersici, Raoiella indica, /¾/77// Carpoglyphidae including Carpoglyphus spp.; Penthaleidae spp. such as Halotydeus destructor, Family Demodicidae with species such as Demodex spp.; Family Trombicidea including Trombicula spp.; Family Macro- nyssidae including Ornothonyssus spp.; Family Pyemotidae including Pyemotes tritici, Tyropha- gus putrescentiae; Family Acaridae including Acarus siro; Family Araneida including Latrodec- tus mactans, Tegenaria agrestis, Chiracanthium sp, Lycosa sp Achaearanea tepidariorum and Loxosceles reclusa,

Pests from the Phylum Nematoda, for example, plant parasitic nematodes such as root-knot nematodes, Meloidogyne spp. such as M. hapla, M. incognita, M. javanica; cyst-forming nematodes, Globodera spp. such as G. rostochiensis; Heterodera spp. such as H. avenae, H. gly- cines, H. schachtii, H. trifo/ii; Seed gall nematodes, Anguina spp:, Stem and foliar nematodes, Aphe/enchoides spp. such as A. besseyi; Sting nematodes, Belonolaimus spp. such as B. lon- gicaudatus; Pine nematodes, Bursaphelenchus spp. such as z?. lignicolus, B. xylophilus; Ring nematodes, Criconema spp., Criconemella spp. such as C. xenoplax and C. ornata; and, Criconemoides spp. such as Criconemoides in formis; Mesocriconema spp.; Stem and bulb nematodes, Ditylenchus spp. such as £>. destructor, D. dipsaci; Awl nematodes, Dolichodorus spp.; Spiral nematodes, Heliocotylenchus multicinctus; Sheath and sheathoid nematodes, A e/77- icycliophora spp. and Hemicriconemoides spp.; Hirshmanniella spp.; Lance nematodes, ¾σ- loaimus spp.; False rootknot nematodes, Nacobbus spp.; Needle nematodes, Longidorus spp. such as Z.. elongatus; Lesion nematodes, Pratylenchus spp. such as . brachyurus, P. neglec- ftys, . penetrans, P. curvitatus, P. goodeyi; Burrowing nematodes, Radopholus spp. such as ?. similis; Rhadopholus spp.; Rhodopholus spp.; Reniform nematodes, Rotylenchus spp. such as ?. robustus, R. reniformis; Scutellonema spp.; Stubby-root nematode, Trichodorus spp. such as 7! obtusus, T. primitivus; Paratrichodorus spp. such as . minor; Stunt nematodes, Tylencho- rhynchus spp. such as 7! claytoni, T. dub/us; Citrus nematodes, Ty/enchu/us spp. such as 7! semipenetrans; Dagger nematodes, Xiphinema spp.; and other plant parasitic nematode species;

Insects from the order Isoptera for example Calotermes flavicollis, Coptotermes spp. such as C. formosanus, C. gestroi, C. acinaciformis; Cornitermes cumuians, Cryptotermes spp. such as C. brevis, C. cavifrons; Globitermes sulfureus, Heterotermes spp. such as A/, aureus, H. longi- ce s, A/, tenuis; Leucotermes flavipes, Odontotermes spp., Incisitermes spp. such as /. /7?/ 7or, /. Snyder, Marginitermes hubbardi, Mastotermes spp. such as M. darwiniensis Neocapritermes spp. such as N. opacus, N. parvus; Neotermes spp., Procornitermes spp., Zootermopsis spp. such as Z. angusticoiiis, Z. nevadensis, Reticuiitermes spp. such as ?. hesperus, R. tibialis, R. speratus, R. flavipes, R. grassei, R. lucifugus, R. santonensis, R. virginicus; Termes natalensis, Insects from the order Blattaria for example Blatta spp. such as B. orientalis, B. lateralis; Blat- tella spp. such as B. asahinae, B. germanica; Leucophaea maderae, Panchlora nivea, Peri- planeta spp. such as P. americana, P. australasiae, P. brunnea, P. fuligginosa, P. japonica; Su- pella longipalpa, Parcoblatta pennsylvanica, Eurycotis floridana, Pycnoscelus surinamensis, Insects from the order Siphonoptera for example Cediopsylla simples, Ceratophyllus spp., Ctenocephalides spp. such as C felis, C cam^'s, Xenopsylla cheopis, Pulex irritans, Tricho- dectes cam^'s, Tung a penetrans, and Nosopsyllus fascia tus,

Insects from the order Thysanura for example Lepisma saccharina , Ctenolepisma urbana, and Thermobia domestica, Pests from the class Chilopoda for example Geophilus spp., Scutigera spp. such as Scutigera coleoptrata,

Pests from the class Diplopoda for example Blaniulus guttulatus, Ju/us spp., Narceus spp., Pests from the class Symphyla for example Scutigerella immaculata,

Insects from the order Dermaptera, for example Forficula auricularia,

Insects from the order Collembola, for example Onychiurus spp., such as Onychiurus armatus, Pests from the order Isopoda for example, Armadillidium vulgare, Oniscus asellus, Porcellio scaber,

Insects from the order Phthiraptera, for example Damalinia spp., Pedicuius spp. such as Pe- diculus humanus capitis, Pedicuius humanus corporis, Pedicuius humanus humanus; Pthirus pubis, Haematopinus spp. such as Haematopinus eurysternus, Haematopinus suis, Linognathus spp. such as Linognathus vituii; Bovicoia bovis, Menopon gaiiinae, Menacanthus stramineus and Solenopotes capillatus, Trichodectes spp.,

Examples of further pest species which may be controlled by compounds of fomula (I) include: from the Phylum Mollusca, class Bivalvia, for example, Dreissena spp.; class Gastropoda, for example, Arion spp., Biomphalaria spp., Bulinus spp., Deroceras spp., Galba spp., Lymnaea spp., Oncomelania spp., Pomacea canaliclata, Succinea spp.; from the class of the helminths, for example, Ancylostoma duodenale, Ancylostoma ceylanicum, Acylostoma braziliensis, Ancy- lostoma spp., Ascaris lubricoides, Ascaris spp., Brugia malayi, Brugia timori, Bunostomum spp., Chabertia spp., Clonorchis spp., Cooper/a spp., Dicrocoelium spp., Dictyocaulus filaria, Diphyl- lobothrium latum, Dracunculus medinensis, Echinococcus granulosus, Echinococcus multilocu- laris, Enterobius vermicularis, Faciola spp., Haemonchus spp. such as Haemonchus contortus; Heterakis spp., Hymenolepis nana, Hyostrongulus spp., Loa Loa, Nematodirus spp., Oesoph- agostomum spp., Opisthorchis spp., Onchocerca volvulus, Ostertagia spp., Paragonim us spp., Schistosomen spp., Strongyloides fuelleborni, Strongyloides stercora lis, Stronyloides spp.,

Taenia saginata, Taenia solium, Trichinella spiralis, Trichinella nativa, Trichinella britovi, Trichi- nella nelsoni, Trichinella pseudopsiralis, Trichostrongulus spp., Trichuris trichuria, Wuchereria bancrofti. The application methods, uses and mixtures of the present invention are particularly useful for controlling insects of the orders Hemiptera and Thysanoptera.

The application methods, uses and mixtures of the present invention are especially suitable for efficiently combating pests like insects from the order of Hemiptera, such as bugs, aphids, leaf- hoppers, plant hoppers, whiteflies, scale insects and cicadas and also thrips from the order of Thysanoptera.

Mixtures of afidopyropen

In one embodiment of the invention, afidopyropen is employed as a solo product.

However, as mentioned in the beginning, one typical problem arising in the field of pest control lies in the general need to reduce the dosage rates of the active ingredient in order to reduce or avoid unfavorable environmental or toxicological effects whilst still allowing effective pest control. Thus the present invention also relates to methods for controlling pests on and/or increasing the plant health of a cultivated plant, comprising in the application of afidopyropen or a mixture of afidopyropen and another active ingredient, e.g. a pesticide compound (II) to a cultivated plant, parts of such plant, plant propagation material or at its locus of growth.

Therefore, in another embodiment of the invention, afidpyropen is employed in combination (e.g. a mixture) with one or more active compound(s) II which is/are preferably a further insecticide or a fungicide.

Insecticides

The compound (II) pesticides, together with which afidopyropen may be used according to the purpose of the present invention, and with which potential synergistic effects with regard to the method of uses might be produced, are selected and grouped according to the Mode of Action Classification from the Insecticde Resistance Action Committee (IRAC) and are selected from group M consisting of:

The following list M of pesticides, grouped and numbered according the Mode of Action Classification of the Insecticide Resistance Action Committee (IRAC), together with which the compounds of the present invention can be used and with which potential synergistic effects might be produced, is intended to illustrate the possible combinations, but not to impose any limitation: M.1 Acetylcholine esterase (AChE) inhibitors from the class of: M.1 A carbamates, for example aldicarb, alanycarb, bendiocarb, benfuracarb, butocarboxim, butoxycarboxim, carbaryl, carbofu- ran, carbosulfan, ethiofencarb, fenobucarb, formetanate, furathiocarb, isoprocarb, methiocarb, methomyl, metolcarb, oxamyl, pirimicarb, propoxur, thiodicarb, thiofanox, trimethacarb, XMC, xylylcarb and triazamate; or from the class of M.1 B organophosphates, for example acephate, azamethiphos, azinphos-ethyl, azinphosmethyl, cadusafos, chlorethoxyfos, chlorfenvinphos, chlormephos, chlorpyrifos, chlorpyrifos-methyl, coumaphos, cyanophos, demeton-S-methyl, diazinon, dichlorvos/ DDVP, dicrotophos, dimethoate, dimethylvinphos, disulfoton, EPN, ethion, ethoprophos, famphur, fenamiphos, fenitrothion, fenthion, fosthiazate, heptenophos, imicyafos, isofenphos, isopropyl O- (methoxyaminothio-phosphoryl) salicylate, isoxathion, malathion, me- carbarn, methamidophos, methidathion, mevinphos, monocrotophos, naled, omethoate, oxyde- meton-methyl, parathion, parathion-methyl, phenthoate, phorate, phosalone, phosmet, phos- phamidon, phoxim, pirimiphos- methyl, profenofos, propetamphos, prothiofos, pyraclofos, pyri- daphenthion, quinalphos, sulfotep, tebupirimfos, temephos, terbufos, tetrachlorvinphos, thi- ometon, triazophos, trichlorfon and vamidothion;

M.2. GABA-gated chloride channel antagonists such as: M.2A cyclodiene organochlorine compounds, as for example endosulfan or chlordane; or M.2B fiproles (phenylpyrazoles), as for example ethiprole, fipronil, flufiprole, pyrafluprole and pyriprole;

M.3 Sodium channel modulators from the class of M.3A pyrethroids, for example acrinathrin, allethrin, d-cis-trans allethrin, d-trans allethrin, bifenthrin, bioallethrin, bioallethrin S- cylclopentenyl, bioresmethrin, cycloprothrin, cyfluthrin, beta-cyfluthrin, cyhalothrin, lambda- cyhalothrin, gamma-cyhalothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, theta- cypermethrin, zeta-cypermethrin, cyphenothrin, deltamethrin, empenthrin, esfenvalerate, etofenprox, fenpropathrin, fenvalerate, flucythrinate, flumethrin, tau-fluvalinate, halfenprox, hep- tafluthrin, imiprothrin, meperfluthrin,metofluthrin, momfluorothrin, permethrin, phenothrin, prallethrin, profluthrin, pyrethrin (pyrethrum), resmethrin, silafluofen, tefluthrin, tetramethylfluth- rin, tetramethrin, tralomethrin and transfluthrin; or M.3B sodium channel modulators such as DDT or methoxychlor;

M.4 Nicotinic acetylcholine receptor agonists (nAChR) from the class of M.4A neonicotinoids, for example acetamiprid, clothianidin, cycloxaprid, dinotefuran, imidacloprid, nitenpyram, thia- cloprid and thiamethoxam; or the compounds M.4A.2: (2E-)-1 -[(6-Chloropyridin-3-yl)methyl]-N'- nitro-2-pentylidenehydrazinecarboximidamide; or M4.A.3: 1 -[(6-Chloropyridin-3-yl)methyl]-7- methyl-8-nitro-5-propoxy-1 ,2,3,5,6,7-hexahydroimidazo[1 ,2-a]pyridine; or from the class M.4B nicotine;

M.5 Nicotinic acetylcholine receptor allosteric activators from the class of spinosyns, for example spinosad or spinetoram;

M.6 Chloride channel activators from the class of avermectins and milbemycins, for example abamectin, emamectin benzoate, ivermectin, lepimectin or milbemectin;

M.7 Juvenile hormone mimics, such as M.7A juvenile hormone analogues as hydroprene, ki- noprene and methoprene; or others as M.7B fenoxycarb or M.7C pyriproxyfen;

M.8 miscellaneous non-specific (multi-site) inhibitors, for example M.8A alkyl halides as methyl bromide and other alkyl halides, or M.8B chloropicrin, or M.8C sulfuryl fluoride, or M.8D borax, or M.8E tartar emetic;

M.9 Selective homopteran feeding blockers, for example M.9B pymetrozine, or M.9C floni- camid;

M.10 Mite growth inhibitors, for example M.10A clofentezine, hexythiazox and diflovidazin, or M.10B etoxazole;

M.1 1 Microbial disruptors of insect midgut membranes, for example bacillus thuringiensis OK bacillus sphaericus and the insecticdal proteins they produce such as bacillus thuringiensis subsp. israelensis, bacillus sphaericus, bacillus thuringiensis subsp. aizawai, bacillus thuringiensis subsp. kurstakiand bacillus thuringiensis subsp. tenebrionis, or the Bt crop proteins: CrylAb, CrylAc, Cryl Fa, Cry2Ab, mCry3A, Cry3Ab, Cry3Bb and Cry34/35Ab1 ;

M.12 Inhibitors of mitochondrial ATP synthase, for example M.12A diafenthiuron, or M.12B or- ganotin miticides such as azocyclotin, cyhexatin or fenbutatin oxide, or M.12C propargite, or M.12D tetrad ifon;

M.13 Uncouplers of oxidative phosphorylation via disruption of the proton gradient, for example chlorfenapyr, DNOC or sulfluramid;

M.14 Nicotinic acetylcholine receptor (nAChR) channel blockers, for example nereistoxin analogues as bensultap, cartap hydrochloride, thiocyclam or thiosultap sodium;

M.15 Inhibitors of the chitin biosynthesis type 0, such as benzoylureas as for example bistriflu- ron, chlorfluazuron, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, novalu- ron, noviflumuron, teflubenzuron or triflumuron;

M.16 Inhibitors of the chitin biosynthesis type 1 , as for example buprofezin;

M.17 Moulting disruptors, Dipteran, as for example cyromazine;

M.18 Ecdyson receptor agonists such as diacylhydrazines, for example methoxyfenozide, tebufenozide, halofenozide, fufenozide or chromafenozide;

M.19 Octopamin receptor agonists, as for example amitraz;

M.20 Mitochondrial complex III electron transport inhibitors, for example M.20A hydramethyl- non, or M.20B acequinocyl, or M.20C fluacrypyrim; M.21 Mitochondrial complex I electron transport inhibitors, for example M.21 A METI acaricides and insecticides such as fenazaquin, fenpyroximate, pyrimidifen, pyridaben, tebufenpyrad or tolfenpyrad, or M.21 B rotenone;

M.22 Voltage-dependent sodium channel blockers, for example M.22A indoxacarb, or M.22B metaflumizone, or M.22B.1 : 2-[2-(4-Cyanophenyl)-1 -[3-(trifluoromethyl)phenyl]ethylidene]-N-[4- (difluoromethoxy)phenyl]-hydrazinecarboxamide or M.22B.2: N-(3-Chloro-2-methylphenyl)-2-[(4- chlorophenyl)[4-[methyl(methylsulfonyl)amino]phenyl]methylene]-hydrazinecarboxamide;

M.23 Inhibitors of the of acetyl CoA carboxylase, such as Tetronic and Tetramic acid derivatives, for example spirodiclofen, spiromesifen or spirotetramat;

M.24 Mitochondrial complex IV electron transport inhibitors, for example M.24A phosphine such as aluminium phosphide, calcium phosphide, phosphine or zinc phosphide, or M.24B cyanide;

M.25 Mitochondrial complex II electron transport inhibitors, such as beta-ketonitrile derivatives, for example cyenopyrafen or cyflumetofen;

M.28 Ryanodine receptor-modulators from the class of diamides, as for example flubendia- mide, chlorantraniliprole (rynaxypyr®), cyantraniliprole (cyazypyr®), tetraniliprole, or the phthalamide compounds M.28.1 : (R)-3-Chlor-N1 -{2-methyl-4-[1 ,2,2,2 -tetrafluor-1 - (trifluormethyl)ethyl]phenyl}-N2-(1 -methyl-2-methylsulfonylethyl)phthalamid and M.28.2: (S)-3- Chlor-N1 -{2-methyl-4-[1 ,2,2,2 -tetrafluor-1 -(trifluormethyl)ethyl]phenyl}-N2-(1 -methyl-2- methylsulfonylethyl)phthalamid, or the compound M.28.3: 3-bromo-N-{2-bromo-4-chloro-6-[(1 - cyclopropylethyl)carbamoyl]phenyl}-1 -(3-chlorpyridin-2-yl)-1 H-pyrazole-5-carboxamide (proposed ISO name: cyclaniliprole), or the compound M.28.4: methyl-2-[3,5-dibromo-2-({[3-bromo- 1 -(3-chlorpyridin-2-yl)-1 H-pyrazol-5-yl]carbonyl}amino)benzoyl]-1 ,2- dimethylhydrazinecarboxylate; or a compound selected from M.28.5a) to M.28.5d) and M.28.5h) to M.28.5I): M.28.5a) N-[4,6-dichloro-2-[(diethyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2- (3-chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide; M.28.5b) N-[4-chloro-2-[(diethyl- lambda-4-sulfanylidene)carbamoyl]-6-methyl-phenyl]-2-(3-chloro-2-pyridyl)-5- (trifluoromethyl)pyrazole-3-carboxamide; M.28.5c) N-[4-chloro-2-[(di-2-propyl-lambda-4- sulfanylidene)carbamoyl]-6-methyl-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3- carboxamide; M.28.5d) N-[4,6-dichloro-2-[(di-2-propyl-lambda-4-sulfanylidene)carbamoyl]- phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide; M.28.5h) N-[4,6- dibromo-2-[(diethyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2-(3-chloro-2-pyridyl)-5- (trifluoromethyl)pyrazole-3-carboxamide; M.28.5i) N-[2-(5-Amino-1 ,3,4-thiadiazol-2-yl)-4-chloro- 6-methylphenyl]-3-bromo-1 -(3-chloro-2-pyridinyl)-1 H-pyrazole-5-carboxamide; M.28.5j) 3- Chloro-1 -(3-chloro-2-pyridinyl)-N-[2,4-dichloro-6-[[(1 -cyano-1 - methylethyl)amino]carbonyl]phenyl]-1 H-pyrazole-5-carboxamide; M.28.5k) 3-Bromo-N-[2,4- dichloro-6-(methylcarbamoyl)phenyl]-1 -(3,5-dichloro-2-pyridyl)-1 H-pyrazole-5-carboxamide; M.28.5I) N-[4-Chloro-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-6-methylphenyl]-1 -(3-chloro-2- pyridinyl)-3-(fluoromethoxy)-1 H-pyrazole-5-carboxamide; or

M.28.6: cyhalodiamide; or;

M.29. insecticidal active compounds of unknown or uncertain mode of action, as for example, afoxolaner, azadirachtin, amidoflumet, benzoximate, bifenazate, broflanilide, bromopropylate, chinomethionat, cryolite, dicloromezotiaz, dicofol, flufenerim, flometoquin, fluensulfone, fluhex- afon, fluopyram, flupyradifurone, fluralaner, metoxadiazone, piperonyl butoxide, pyflubumide, pyridalyl, pyrifluquinazon, sulfoxaflor, tioxazafen, triflumezopyrim, or the compounds

M.29.3: 1 1 -(4-chloro-2,6-dimethylphenyl)-12-hydroxy-1 ,4-dioxa-9-azadispiro[4.2.4.2]-tetradec- 1 1 -en-10-one, or the compound

M.29.4: 3-(4'-fluoro-2,4-dimethylbiphenyl-3-yl)-4-hydroxy-8-oxa-1 -azaspiro[4.5]dec-3-en-2-one, or the compound

M.29.5: 1 -[2-fluoro-4-methyl-5-[(2,2,2-trifluoroethyl)sulfinyl]phenyl]-3-(trifluoromethyl)-1 H-1 ,2,4- triazole-5-amine, or actives on basis of bacillus firmus (Votivo, 1-1582); or

a compound selected from the group of M.29.6, wherein the compound is selected from M.29.6a) to M.29.6k): M.29.6a) (E/Z)-N-[1 -[(6-chloro-3-pyridyl)methyl]-2-pyridylidene]-2,2,2- trifluoro-acetamide; M.29.6b) (E/Z)-N-[1 -[(6-chloro-5-fluoro-3-pyridyl)methyl]-2-pyridylidene]- 2,2,2-trifluoro-acetamide; M.29.6c) (E/Z)-2,2,2-trifluoro-N-[1 -[(6-fluoro-3-pyridyl)methyl]-2- pyridylidene]acetamide; M.29.6d) (E/Z)-N-[1 -[(6-bromo-3-pyridyl)methyl]-2-pyridylidene]-2,2,2- trifluoro-acetamide; M.29.6e) (E/Z)-N-[1 -[1 -(6-chloro-3-pyridyl)ethyl]-2-pyridylidene]-2,2,2- trifluoro-acetamide; M.29.6f) (E/Z)-N-[1 -[(6-chloro-3-pyridyl)methyl]-2-pyridylidene]-2,2-difluoro- acetamide; M.29.6g) (E/Z)-2-chloro-N-[1 -[(6-chloro-3-pyridyl)methyl]-2-pyridylidene]-2,2-difluoro- acetamide; M.29.6h) (E/Z)-N-[1 -[(2-chloropyrimidin-5-yl)methyl]-2-pyridylidene]-2,2,2-trifluoro- acetamide; M.29.6i) (E/Z)-N-[1 -[(6-chloro-3-pyridyl)methyl]-2-pyridylidene]-2,2,3,3,3-pentafluoro- propanamide.); M.29.6j) N-[1 -[(6-chloro-3-pyridyl)methyl]-2-pyridylidene]-2,2,2-trifluoro- thioacetamide; or M.29.6k) N-[1 -[(6-chloro-3-pyridyl)methyl]-2-pyridylidene]-2,2,2-trifluoro-N'- isopropyl-acetamidine; or the compounds

M.29.8: fluazaindolizine; or the compounds

M.29.9.a): 4-[5-(3,5-dichlorophenyl)-5-(trifluoromethyl)-4H-isoxazol-3-yl]-2-methyl-N-(1 - oxothietan-3-yl)benzamide; or M.29.9.b): fluxametamide; or

M.29.10: 5-[3-[2,6-dichloro-4-(3,3-dichloroallyloxy)phenoxy]propoxy]-1 H-pyrazole; or a compound selected from the group of M.29.1 1 , wherein the compound is selected from M.29.1 1 b) to M.29.1 1 p): M.29.1 1 .b) 3-(benzoylmethylamino)-N-[2-bromo-4-[1 , 2,2,3,3,3- hexafluoro-1 -(trifluoromethyl)propyl]-6-(trifluoromethyl)phenyl]-2-fluoro-benzamide; M.29.1 1.c)

3- (benzoylmethylamino)-2-fluoro-N-[2-iodo-4-[1 ,2,2,2-tetrafluoro-1 -(trifluoromethyl)ethyl]-6- (trifluoromethyl)phenyl]-benzamide; M.29.1 1 .d) N-[3-[[[2-iodo-4-[1 ,2,2,2-tetrafluoro-1 -

(trifluoromethyl)ethyl]-6-(trifluoromethyl)phenyl]amino]carbonyl]phenyl]-N-methyl-benzamide; M.29.1 1.e) N-[3-[[[2-bromo-4-[1 ,2,2,2-tetrafluoro-1-(trifluoromethyl)ethyl]-6- (trifluoromethyl)phenyl]amino]carbonyl]-2-fluorophenyl]-4-fluoro-N-methyl-benzamide; M.29.1 1.f)

4- fluoro-N-[2-fluoro-3-[[[2-iodo-4-[1 ,2,2,2-tetrafluoro-1 -(trifluoromethyl)ethyl]-6- (trifluoromethyl)phenyl]amino]carbonyl]phenyl]-N-methyl-benzamide; M.29.1 1.g) 3-fluoro-N-[2- fluoro-3-[[[2-iodo-4-[1 ,2,2,2-tetrafluoro-1 -(trifluoromethyl)ethyl]-6-

(trifluoromethyl)phenyl]amino]carbonyl]phenyl]-N-methyl-benzamide; M.29.1 1.h) 2-chloro-N-[3- [[[2-iodo-4-[1 ,2,2,2-tetrafluoro-1 -(trifluoromethyl)ethyl]-6-

(trifluoromethyl)phenyl]amino]carbonyl]phenyl]- 3-pyridinecarboxamide; M.29.1 1.i) 4-cyano-N- [2-cyano-5-[[2,6-dibromo-4-[1 ,2,2,3,3,3-hexafluoro-1 -(trifluoromethyl)- propyl]phenyl]carbamoyl]phenyl]-2-methyl-benzamide; M.29.1 1.j) 4-cyano-3-[(4-cyano-2-methyl- benzoyl)amino]-N-[2,6-dichloro-4-[1 ,2,2,3,3,3-hexafluoro-1 -(trifluoromethyl)propyl]phenyl]-2- fluoro-benzamide; M.29.1 1.k) N-[5-[[2-chloro-6-cyano-4-[1 ,2,2,3,3,3-hexafluoro-1 - (trifluoromethyl)propyl]phenyl]carbamoyl]-2-cyano-phenyl]-4-cyano-2-methyl-benzamide; M.29.1 1 .1) N-[5-[[2-bromo-6-chloro-4-[2,2,2-trifluoro-1 -hydroxy-1 - (trifluoromethyl)ethyl]phenyl]carbamoyl]-2-cyano-phenyl]-4-cyano-2-methyl-benzamide;

M.29.1 1 .m) N-[5-[[2-bromo-6-chloro-4-[1 ,2,2,3,3,3-hexafluoro-1 -(trifluoromethyl)- propyl]phenyl]carbamoyl]-2-cyano-phenyl]-4-cyano-2-methyl-benzamide; M.29.1 1 .n) 4-cyano-N- [2-cyano-5-[[2,6-dichloro-4-[1 ,2,2,3,3,3-hexafluoro-1 -(trifluoromethyl)- propyl]phenyl]carbamoyl]phenyl]-2-methyl-benzamide; M.29.1 1 .o) 4-cyano-N-[2-cyano-5-[[2,6- dichloro-4-[1 ,2,2,2-tetrafluoro-1 -(trifluoromethyl)ethy^

benzamide; M.29.1 1 .p) N-[5-[[2-bromo-6-chloro-4-[1 ,2,2,2-tetrafluoro-1 -

(trifluoromethyl)ethyl]phenyl]carbamoyl]-2-cyano-phenyl]-4-cyano-2-methyl-benzamide; or a compound selected from the group of M.29.12, wherein the compound is selected from M.29.12a) to M.29.12m): M.29.12.a) 2-(1 ,3-Dioxan-2-yl)-6-[2-(3-pyridinyl)-5-thiazolyl]-pyridine; M.29.12. b) 2-[6-[2-(5-Fluoro-3-pyridinyl)-5-thiazolyl]-2-pyridinyl]-pyrimidine; M.29.12. c) 2-[6-[2- (3-Pyridinyl)-5-thiazolyl]-2-pyridinyl]-pyrimidine; M.29.12.d) N-Methylsulfonyl-6-[2-(3- pyridyl)thiazol-5-yl]pyridine-2-carboxamide; M.29.12.e) N-Methylsulfonyl-6-[2-(3-pyridyl)thiazol- 5-yl]pyridine-2-carboxamide; M.29.12.f) N-Ethyl-N-[4-methyl-2-(3-pyridyl)thiazol-5-yl]-3- methylthio-propanamide; M.29.12.g) N-Methyl-N-[4-methyl-2-(3-pyridyl)thiazol-5-yl]-3- methylthio-propanamide; M.29.12. h) N,2-Dimethyl-N-[4-methyl-2-(3-pyridyl)thiazol-5-yl]-3- methylthio-propanamide; M.29.12.i) N-Ethyl-2-methyl-N-[4-methyl-2-(3-pyridyl)thiazol-5-yl]-3- methylthio-propanamide; M.29.12.j) N-[4-Chloro-2-(3-pyridyl)thiazol-5-yl]-N-ethyl-2-methyl-3- methylthio-propanamide; M.29.12.k) N-[4-Chloro-2-(3-pyridyl)thiazol-5-yl]-N,2-dimethyl-3- methylthio-propanamide; M.29.12.1) N-[4-Chloro-2-(3-pyridyl)thiazol-5-yl]-N-methyl-3-methylthio- propanamide; M.29.12.m) N-[4-Chloro-2-(3-pyridyl)thiazol-5-yl]-N-ethyl-3-methylthio- propanamide; or the compounds

M.29.14a) 1 -[(6-Chloro-3-pyridinyl)methyl]-1 ,2,3,5, 6,7-hexahydro-5-methoxy-7-methyl-8-nitro- imidazo[1 ,2-a]pyridine; or M.29.14b) 1 -[(6-Chloropyridin-3-yl)methyl]-7-methyl-8-nitro- 1 ,2,3,5,6,7-hexahydroimidazo[1 ,2-a]pyridin-5-ol; or the compounds

M.29.16a) 1 -isopropyl-N,5-dimethyl-N-pyridazin-4-yl-pyrazole-4-carboxamide; or M.29.16b) 1 - (1 ,2-dimethylpropyl)-N-ethyl-5-methyl-N-pyridazin-4-yl-pyrazole-4-carboxamide; M.29.16c) N,5- dimethyl-N-pyridazin-4-yl-1 -(2,2,2-trifluoro-1 -methyl-ethyl)pyrazole-4-carboxamide; M.29.16d) 1 - [1 -(1 -cyanocyclopropyl)ethyl]-N-ethyl-5-methyl-N-pyridazin-4-yl-pyrazole-4-carboxamide;

M.29.16e) N-ethyl-1 -(2-fluoro-1 -methyl-propyl)-5-methyl-N-pyridazin-4-yl-pyrazole-4- carboxamide; M.29.16f) 1 -(1 ,2-dimethylpropyl)-N,5-dimethyl-N-pyridazin-4-yl-pyrazole-4- carboxamide; M.29.16g) 1 -[1 -(1 -cyanocyclopropyl)ethyl]-N,5-dimethyl-N-pyridazin-4-yl-pyrazole- 4-carboxamide; M.29.16h) N-methyl-1 -(2-fluoro-1 -methyl-propyl]-5-methyl-N-pyridazin-4-yl- pyrazole-4-carboxamide; M.29.16i) 1 -(4,4-difluorocyclohexyl)-N-ethyl-5-methyl-N-pyridazin-4-yl- pyrazole-4-carboxamide; or M.29.16j) 1 -(4,4-difluorocyclohexyl)-N,5-dimethyl-N-pyridazin-4-yl- pyrazole-4-carboxamide, or

M.29.17 a compound selected from the compounds M.29.17a) to M.29.17j): M.29.17a) N-(1 - methylethyl)-2-(3-pyridinyl)-2H-indazole-4-carboxamide; M.29.17b) N-cyclopropyl-2-(3- pyridinyl)-2H-indazole-4-carboxamide; M.29.17c) N-cyclohexyl-2-(3-pyridinyl)-2H-indazole-4- carboxamide; M.29.17d) 2-(3-pyridinyl)-N-(2,2,2-trifluoroethyl)-2H-indazole-4-carboxamide; M.29.17e) 2-(3-pyridinyl)-N-[(tetrahydro-2-furanyl)methyl]-2H-indazole-5-carboxamide;

M.29.17f) methyl 2-[[2-(3-pyridinyl)-2H-indazol-5-yl]carbonyl]hydrazinecarboxylate; M.29.17g) N- [(2,2-difluorocyclopropyl)methyl]-2-(3-pyridinyl)-2H-indazole-5-carboxamide; M.29.17h) N-(2,2- difluoropropyl)-2-(3-pyridinyl)-2H-indazole-5-carboxamide; M.29.17i) 2-(3-pyridinyl )-N-(2- pyrimidinylmethyl )-2H-indazole-5-carboxamide; M.29.17j) N-[(5-methyl-2-pyrazinyl)methyl]-2- (3-pyridinyl)-2H-indazole-5-carboxamide, or

M.29.18 a compound selected from the compounds M.29.18a) to M.29.18d): M.29.18a) N-[3- chloro-1 -(3-pyridyl)pyrazol-4-yl]-N-ethyl-3-(3,3,3-trifluoropropylsulfanyl)propanamide; M.29.18b) N-[3-chloro-1 -(3-pyridyl)pyrazol-4-yl]-N-ethyl-3-(3,3,3-trifluoropropylsulfinyl)propanamide;

M.29.18c) N-[3-chloro-1 -(3-pyridyl)pyrazol-4-yl]-3-[(2,2-difluorocyclopropyl)methylsulfanyl]-N- ethyl-propanamide; M.29.18d) N-[3-chloro-1 -(3-pyridyl)pyrazol-4-yl]-3-[(2,2- difluorocyclopropyl)methylsulfinyl]-N-ethyl-propanamide; or the compound

M.29.19 sarolaner, or the compound

M.29.20 lotilaner.

The commercially available compounds of the group M listed above may be found in The Pesticide Manual, 16th Edition, C. MacBean, British Crop Protection Council (2013) among other publications. The online Pesticide Manual is updated regularly and is accessible through http://bcpcdata.com/pesticide-manual.html.

Another online data base for pesticides providing the ISO common names is

http://www.alanwood.net/pesticides.

The M.4 neonicotinoid cycloxaprid is known from WO2010/069266 and WO201 1/069456, the neonicotinoid M.4A.2, sometimes also to be named as guadipyr, is known from

WO2013/003977, and the neonicotinoid M.4A.3 (approved as paichongding in China) is known from WO2007/101369. The metaflumizone analogue M.22B.1 is described in CN10171577 and the analogue M.22B.2 in CN102126994. The phthalamides M.28.1 and M.28.2 are both known from WO2007/101540. The anthranilamide M.28.3 is described in WO2005/077934. The hydra- zide compound M.28.4 is described in WO2007/043677. The anthranilamides M.28.5a) to M.28.5d) and M.28.5h) are described in WO 2007/006670, WO2013/024009 and

WO2013/024010, the anthranilamide Μ.28.5Ϊ) is described in WO201 1/085575, M.28.5j) in WO2008/134969, M.28.5k) in US201 1/046186 and M.28.5I) in WO2012/034403. The diamide compound M.28.6 can be found in WO2012/034472. The spiroketal-substituted cyclic ketoenol derivative M.29.3 is known from WO2006/089633 and the biphenyl-substituted spirocyclic ke- toenol derivative M.29.4 from WO2008/06791 1. The triazoylphenylsulfide M.29.5 is described in WO2006/043635, and biological control agents on the basis of bacillus firmus are described in WO2009/124707. The compounds M.29.6a) to Μ.29.6Ϊ) listed under M.29.6 are described in WO2012/029672, and M.29.6j) and M.29.6k) in WO2013/129688. The nematicide M.29.8 is known from WO2013/055584. The isoxazoline M.29.9.a) is described in WO2013/050317. The isoxazoline M.29.9.b) is described in WO2014/126208. The pyridalyl-type analogue M.29.10 is known from WO2010/060379. The carboxamides broflanilide and M.29.1 1.b) to M.29.1 1 .h) are described in WO2010/018714, and the carboxamides M.29.1 1 i) to M.29.1 1.p) in

WO2010/127926. The pyridylthiazoles M.29.12.a) to M.29.12.C) are known from

WO2010/006713, M.29.12.d) and M.29.12.e) are known from WO2012/000896, and M.29.12.†) to M.29.12.m) from WO2010/129497. The compounds M.29.14a) and M.29.14b) are known from WO2007/101369. The pyrazoles M.29.16.a) to M.29.16h) are described in

WO2010/034737, WO2012/084670, and WO2012/143317, respectively, and the pyrazoles Μ.29.16Ϊ) and M.29.16j) are described in US 61/891437. The pyridinylindazoles M.29.17a) to M.29.17J) are described in WO2015/038503. The pyridylpyrazoles M.29.18a) to M.29.18d) are described in US2014/0213448. The isoxazoline M.29.19 is described in WO2014/036056. The isoxazoline M.29.20 is known from WO2014/090918.

With regard to the use of a mixture of afidopyropen with another insecticide in the methods according to the present invention, the following mixtures are preferred:

With regard to the use of a pesticidal mixture comprising afidopyropen, compound II selected from group M.2 (GABA-gated chloride channel antagonists) as defined above is preferred, in particularfrom group M.2B (fiproles). Especially preferred are ethiprole and fipronil.

Mixtures of with fipronil as compound II are particularly preferred.

With regard to the use of a pesticidal mixture comprising afidopyropen, compound II selected from group M.3 (Sodium channel modulators) as defined above is preferred, in particular from group M.3A (pyrethroids). Especially preferred are alpha-cypermethrin, bifenthrin and cyhalo- thrin.

Mixtures of with alpha-cypermethrin as compound II are particularly preferred.

With regard to the use of a pesticidal mixture comprising afidopyropen, compound II selected from group M.4A (Neonicotinoids) as defined above is preferred, in particular clothianidin, di- notefuran, imidacloprid, thiacloprid, or thiamethoxam.

Mixtures with dinotefuran as compound II are especially preferred.

With regard to the use of a pesticidal mixture comprising afidopyropen, the compound II select- ed from group M.5 (Nicotinic acetylcholine receptor allosteric activators) as defined above is preferred, in particular spinosad or spinetoram.

With regard to the use of a pesticidal mixture comprising afidopyropen, compound II selected from group M.6 (Chloride channel activators) as defined above is preferred, in particular aver- mectin.

Mixtures of with abamectin as compound II are especially preferred.

With regard to the use of a pesticidal mixture comprising afidopyropen, compound II selected from group M.9 (Selective homopteran feeding blockers) as defined above is preferred, in par- ticular pymetrozine or flonicamid.

Mixtures with pymetrozine as compound II are especially preferred.

Mixtures with flonicamid as compound II are also preferred.

With regard to the use of a pesticidal mixture comprising afidopyropen, compound II selected from group M.13 (Uncouplers of oxidative phosphorylation via disruption of the proton gradient) as defined above is preferred, in particular chlorfenapyr.

Mixtures with chlorfenapyr as compound II are especially preferred. With regard to the use of a pesticidal mixture comprising afidopyropen, compound II selected from group M.16 (Inhibitors of the chitin biosynthesis type 1 ) as defined above is preferred, in particular buprofezin. With regard to the use of a pesticidal mixture comprising afidopyropen, the compound II selected from group M.22 (Voltage-dependent sodium channel blockers) as defined above is preferred, in particular metaflumizone.

With regard to the use of a pesticidal mixture comprising afidopyropen, the compound II select- ed from group M.23 (Inhibitors of the of acetyl CoA carboxylase) as defined above is preferred, in particular a Tetronic or Tetramic acid derivative, spirodiclofen, spiromesifen or spirotetramat. Mixtures with Tetronic Acid as compound II are preferred.

Mixtures with Tetramic Acid as compound II are also preferred. With regard to the use of a pesticidal mixture comprising afidopyropen, compound II selected from group M.28 (Ryanodine receptor-modulators) as defined above is preferred, in particular chloranthraniliprole, cyananthraniliprole, N-[4,6-dichloro-2-[(diethyl-lambda-4- sulfanylidene)carbamoyl]-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3- carboxamide, N-[4-chloro-2-[(diethyl-lambda-4-sulfanylidene)carbamoyl]-6-methyl-phenyl]-2-(3- chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide, N-[4-chloro-2-[(di-2-propyl-lambda- 4-sulfanylidene)carbamoyl]-6-methyl-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3- carboxamide, N-[4,6-dichloro-2-[(di-2-propyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2-(3- chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide, N-[4,6-dichloro-2-[(diethyl-lambda- 4-sulfanylidene)carbamoyl]-phenyl]-2-(3-chloro-2-pyridyl)-5-(difluoromethyl)pyrazole-3- carboxamide, N-[4,6-dibromo-2-[(di-2-propyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2-(3- chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide, N-[4-chloro-2-[(di-2-propyl-lambda- 4-sulfanylidene)carbamoyl]-6-cyano-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3- carboxamide, N-[4,6-dibromo-2-[(diethyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2-(3- chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide or 1 -(3-Chloro-2-pyridinyl)-N-[4- cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-3-[[5-(trifluoromethyl)-2H-tetrazol-2- yl]methyl]-1 H-pyrazole-5-carboxamide (tetraniliprole).

Mixtures with N-[4,6-dichloro-2-[(diethyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2-(3-chloro- 2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide as compound II are especially preferred. Mixtures with N-[4-chloro-2-[(diethyl-lambda-4-sulfanylidene)carbamoyl]-6-methyl-phenyl]-2-(3- chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide as compound II are especially preferred.

Mixtures with N-[4-chloro-2-[(di-2-propyl-lambda-4-sulfanylidene)carbamoyl]-6-methyl-phenyl]-2- (3-chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide as compound II are especially preferred.

With regard to the use of a pesticidal mixture comprising afidopyropen, compound II selected from group M.29 as defined above is preferred, in particular selected from the group conisisting of sulfoxaflor, broflanilide and a carboxamide of subgroup M.29.1 1 , such as M.29.1 1 .b) 3-(benzoylmethylamino)-N-[2-bromo-4-[1 ,2,2,3,3,3-hexafluoro-1 -

(trifluoromethyl)propyl]-6-(trifluoromethyl)phenyl]-2-fluoro-benzamide;

M.29.1 1.C) 3-(benzoylmethylamino)-2-fluoro-N-[2-iodo-4-[1 ,2,2,2-tetrafluoro-1 -

(trifluoromethyl)ethyl]-6-(trifluoromethyl)phenyl]-benzamide;

M.29.1 1 .d) N-[3-[[[2-iodo-4-[1 ,2,2,2-tetrafluoro-1 -(trifluoromethyl)ethyl]-6-

(trifluoromethyl)phenyl]amino]carbonyl]phenyl]-N-methyl-benzamide;

M.29.1 1 .e) N-[3-[[[2-bromo-4-[1 ,2,2,2-tetrafluoro-1-(trifluoromethyl)ethyl]-6-

(trifluoromethyl)phenyl]amino]carbonyl]-2-fluorophenyl]-4-fluoro-N-methyl-ben

M.29.1 1 .f) 4-fluoro-N-[2-fluoro-3-[[[2-iodo-4-[1 ,2,2,2-tetrafluoro-1 -(trifluoromethyl)ethyl]-6- (trifluoromethyl)phenyl]amino]carbonyl]phenyl]-N-methyl-benzamide;

M.29.1 1.g) 3-fluoro-N-[2-fluoro-3-[[[2-iodo-4-[1 ,2,2,2-tetrafluoro-1 -(trifluoromethyl)ethyl]-6-

(trifluoromethyl)phenyl]amino]carbonyl]phenyl]-N-methyl-benzamide;

M.29.1 1.h) 2-chloro-N-[3-[[[2-iodo-4-[1 ,2,2,2-tetrafluoro-1 -(trifluoromethyl)ethyl]-6-

(trifluoromethyl)phenyl]amino]carbonyl]phenyl]- 3-pyridinecarboxamide;

M.29.1 1 .i) 4-cyano-N-[2-cyano-5-[[2,6-dibromo-4-[1 ,2,2,3,3,3-hexafluoro-1 -

(trifluoromethyl)propyl]phenyl]carbamoyl]phenyl]-2-methyl-benzamide;

M.29.1 1 .j) 4-cyano-3-[(4-cyano-2-methyl-benzoyl)amino]-N-[2,6-dichloro-4-[1 ,2,2,3,3,3- hexafluoro-1 -(trifluoromethyl)propyl]phenyl]-2-fluoro-benzamide;

M.29.1 1 .k) N-[5-[[2-chloro-6-cyano-4-[1 ,2,2,3,3,3-hexafluoro-1 - (trifluoromethyl)propyl]phenyl]carbamoyl]-2-cyano-phenyl]-4-cyano-2-methyl-ben

M.29.1 1 .1) N-[5-[[2-bromo-6-chloro-4-[2,2,2-trifluoro-1 -hydroxy-1 -

(trifluoromethyl)ethyl]phenyl]carbamoyl]-2-cyano-phenyl]-4-cyano-2-methyl-ben

M.29.1 1.m) N-[5-[[2-bromo-6-chloro-4-[1 ,2,2,3,3,3-hexafluoro-1 -

(trifluoromethyl)propyl]phenyl]carbamoyl]-2-cyano-phenyl]-4-cyano-2-methyl-ben

M.29.1 1.n) 4-cyano-N-[2-cyano-5-[[2,6-dichloro-4-[1 ,2,2,3,3,3-hexafluoro-1 -

(trifluoromethyl)propyl]phenyl]carbamoyl]phenyl]-2-methyl-benzamide;

M.29.1 1.o) 4-cyano-N-[2-cyano-5-[[2,6-dichloro-4-[1 ,2,2,2-tetrafluoro-1 -

(trifluoromethyl)ethyl]phenyl]carbamoyl]phenyl]-2-methyl-benzamide; and

M.29.1 1 .p) N-[5-[[2-bromo-6-chloro-4-[1 ,2,2,2-tetrafluoro-1 - (trifluoromethyl)ethyl]phenyl]carbamoyl]-2-cyano-phenyl]-4-cyano-2-methyl-benzamide.

Mixtures of afidopyropenewith sulfoxaflor as compound II are especially preferred.

Mixtures of afidopyropene with compound 3-[benzoyl(methyl)amino]-N-[2-bromo-4-[1 , 2,2,2- tetrafluoro-1 -(trifluoromethyl)ethyl]-6-(trifluoromethyl)phenyl]-2-fluoro-benzamide as compound II are especially preferred.

According to the methods of the present inventions, the afidopyropen may also be applied together with a fungicide selected from the following group F consisting of:

A) Respiration inhibitors

Inhibitors of complex III at Q₀ site (e. g. strobilurins): azoxystrobin (A.1 .1 ), coumethoxy- strobin (A.1.2), coumoxystrobin (A.1 .3), dimoxystrobin (A.1.4), enestroburin (A.1 .5), fenamin- strobin (A.1 .6), fenoxystrobin/flufenoxystrobin (A.1 .7), fluoxastrobin (A.1 .8), kresoxim-methyl (A.1.9), mandestrobin (A.1.10), metominostrobin (A.1.1 1 ), orysastrobin (A.1.12), picoxy.strobin (A.1.13), pyraclostrobin (A.1 .14), pyrametostrobin (A.1.15), pyraoxystrobin (A.1.16), tri- floxystrobin (A.1 .17), 2-(2-(3-(2,6-dichlorophenyl)-1 -methyl-allylideneaminooxymethyl)-phenyl)- 2- methoxyimino-N-methyl-acetamide (A.1.18), pyribencarb (A.1.19), tnclopyricarb/chlorodincarb (A.1.20), famoxadone (A.1 .21 ), fenamidone (A.1 .21 ), methyl- V-[2-[(1 ,4-dimethyl-5-phenyl- pyrazol-3-yl)oxylmethyl]phenyl]-N-methoxy-carbamate (A.1 .22), 1 -[3-chloro-2-[[1 -(4- chlorophenyl)-1 H-pyrazol-3-yl]oxymethyl]phenyl]-4-methyl-tetrazol-5-one (A.1 .23), 1 -[3-bromo- 2-[[1 -(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-4-methyl-tetrazol-5-one (A.1.24), 1 -[2-[[1 - (4-chlorophenyl)pyrazol-3-yl]oxymethyl]-3-methyl-phenyl]-4-methyl-tetrazol-5-one (A.1 .25), 1 -[2- [[1 -(4-chlorophenyl)pyrazol-3-yl]oxymethyl]-3-fluoro-phenyl]-4-methyl-tetrazol-5-one (A.1 .26), 1 - [2-[[1 -(2,4-dichlorophenyl)pyrazol-3-yl]oxymethyl]-3-fluoro-phenyl]-4-methyl-tetrazol-5-one (A.1.27), 1 -[2-[[4-(4-chlorophenyl)thiazol-2-yl]oxymethyl]-3-methyl-phenyl]-4-methyl-tetrazol-5- one (A.1 .28), 1 -[3-chloro-2-[[4-(p-tolyl)thiazol-2-yl]oxymethyl]phenyl]-4-methyl-tetrazol-5-one (A.1.29), 1 -[3-cyclopropyl-2-[[2-methyl-4-(1 -methylpyrazol-3-yl)phenoxy]methyl]phenyl]- 4-methyl-tetrazol-5-one (A.1 .30), 1 -[3-(difluoromethoxy)-2-[[2-methyl-4-(1 -methylpyrazol-

3- yl)phenoxy]methyl]phenyl]-4-methyl-tetrazol-5-one (A.1.31 ), 1 -methyl-4-[3-methyl-

2- [[2-methyl-4-(1 -methylpyrazol-3-yl)phenoxy]methyl]phenyl]tetrazol-5-one (A.1.32), 1 -methyl-4- [3-methyl-2-[[1 -[3-(trifluoromethyl)phenyl]-ethylideneamino]oxymethyl]phenyl]tetrazo

(A.1.33), (Z;2£)-5-[1 -(2,4-dichlorophenyl)pyrazol-3-yl^

enamide (A.1.34), (^2 )-5-[1 -(4-chlorophenyl)pyrazol-3-yl]oxy-2-methoxyimino- V,3-dimethyl- pent-3-enamide (A.1 .35), (^2 )-5-[1 -(4-chloro-2-fluoro-phenyl)pyrazol-3-yl]oxy-2- methoxyimino- V,3-dimethyl-pent-3-enamide (A.1.36),

- inhibitors of complex III at Qi site: cyazofamid (A.2.1 ), amisulbrom (A.2.2), [(3S,6S,7R,8R)- 8-benzyl-3-[(3-acetoxy-4-methoxy-pyridine-2-carbonyl)amino]-6-methyl-4,9-dioxo-1 ,5-dioxonan- 7-yl] 2-methylpropanoate (A.2.3), [(3S,6S,7R,8R)-8-benzyl-3-[[3-(acetoxymethoxy)-4-methoxy- pyridine-2-carbonyl]amino]-6-methyl-4,9-dioxo-1 ,5-dioxonan-7-yl] 2-methylpropanoate (A.2.4), [(3S,6S,7R,8R)-8-benzyl-3-[(3-isobutoxycarbonyloxy-4-methoxy-pyridine-2-carbonyl)amino]-6- methyl-4,9-dioxo-1 ,5-dioxonan-7-yl] 2-methylpropanoate (A.2.5), [(3S,6S,7R,8R)-8-benzyl-3-[[3- (1 ,3-benzodioxol-5-ylmethoxy)-4-methoxy-pyridine-2-carbonyl]amino]-6-methyl-4,9-dioxo-1 ,5- dioxonan-7-yl] 2-methylpropanoate (A.2.6); (3S,6S,7R,8R)-3-[[(3-hydroxy-4-methoxy-2- pyridinyl)carbonyl]amino]-6-methyl-4,9-dioxo-8-(phenylmethyl)-1 ,5-dioxonan-7-yl 2- methylpropanoate (A.2.7), (3S,6S,7R,8R)-8-benzyl-3-[3-[(isobutyryloxy)methoxy]-4- methoxypicolinamido]-6-methyl-4,9-dioxo-1 ,5-dioxonan-7-yl isobutyrate (A.2.8);

inhibitors of complex II (e. g. carboxamides): benodanil (A.3.1 ), benzovindiflupyr (A.3.2), bixafen (A.3.3), boscalid (A.3.4), carboxin (A.3.5), fenfuram (A.3.6), fluopyram (A.3.7), flutolanil (A.3.8), fluxapyroxad (A.3.9), furametpyr (A.3.10), isofetamid (A.3.1 1 ), isopyrazam (A.3.12), mepronil (A.3.13), oxycarboxin (A.3.14), penflufen (A.3.14), penthiopyrad (A.3.15), sedaxane (A.3.16), tecloftalam (A3.17), thifluzamide (A3.18), N-(4'-trifluoromethylthiobiphenyl-2-yl)-

3- difluoromethyl-1 -methyl-1 H-pyrazole-4-carboxamide (A.3.19), N-(2-(1 ,3,3-trimethyl-butyl)- phenyl)-1 ,3-dimethyl-5-fluoro-1 H-pyrazole-4-carboxamide (A.3.20), 3-(difluoromethyl)-1 -methyl- N-(1 ,1 ,3-trimethylindan-4-yl)pyrazole-4-carboxamide (A.3.21 ), 3-(trifluoromethyl)-1 -methyl-Nil ,1 ,3-trimethylindan-4-yl)pyrazole-4-carboxamide (A.3.22), 1 ,3-dimethyl-N-(1 ,1 ,3- trimethylindan-4-yl)pyrazole-4-carboxamide (A.3.23), 3-(trifluoromethyl)-1 ,5-dimethyl-N-(1 ,1 ,3- trimethylindan-4-yl)pyrazole-4-carboxamide (A.3.24), 1 ,3,5-trimethyl-N-(1 ,1 ,3-trimethylindan-4- yl)pyrazole-4-carboxamide (A.3.25), N-(7-fluoro-1 ,1 ,3-trimethyl-indan-4-yl)-1 ,3-dimethyl- pyrazole-4-carboxamide (A.3.26), N-[2-(2,4-dichlorophenyl)-2-methoxy-1 -methyl-ethyl]-3- (difluoromethyl)-l -methyl-pyrazole-4-carboxamide (A.3.27); other respiration inhibitors (e. g. complex I, uncouplers): diflumetorim (A.4.1 ), (5,8-difluoro- quinazolin-4-yl)-{2-[2-fluoro-4-(4-trifluoromethylpyridin-2-yloxy)-phenyl]-ethyl}-amine (A.4.2); nitrophenyl derivates: binapacryl (A.4.3), dinobuton (A.4.4), dinocap (A.4.5), fluazinam (A.4.6); ferimzone (A.4.7); organometal compounds: fentin salts, such as fentin-acetate (A.4.8), fentin chloride (A.4.9) or fentin hydroxide (A.4.10); ametoctradin (A.4.1 1 ); and silthiofam (A.4.12);

B) Sterol biosynthesis inhibitors (SBI fungicides)

C14 demethylase inhibitors (DMI fungicides): triazoles: azaconazole (B.1.1 ), bitertanol (B.1.2), bromuconazole (B.1.3), cyproconazole (B.1 .4), difenoconazole (B.1 .5), diniconazole (B.1.6), diniconazole-M (B.1 .7), epoxiconazole (B.1.8), fenbuconazole (B.1 .9), fluquinconazole (B.1.10), flusilazole (B.1 .1 1 ), flutriafol (B.1 .12), hexaconazole (B.1.13), imibenconazole (B.1.14), ipconazole (B.1.15), metconazole (B.1 .17), myclobutanil (B.1 .18), oxpoconazole (B.1.19), paclobutrazole (B.1 .20), penconazole (B.1.21 ), propiconazole (B.1 .22), prothioconazole

(B.1.23), simeconazole (B.1 .24), tebuconazole (B.1.25), tetraconazole (B.1 .26), triadimefon (B.1.27), triadimenol (B.1 .28), triticonazole (B.1.29), uniconazole (B.1 .30), -[ΓΘΙ-{2 &,2>Ρ?)-2>-{2- chlorophenyl)-2-(2,4-difluorophenyl)-oxiranylmethyl]-5-thiocyanato-1 H-[1 ,2,4]triazolo (B.1 .31 ), 2- [/-eA(2^3 )-3-(2-chlorophenyl)-2-(2,4-difluorophenyl)-oxiranylmethyl]-2H-[1 ,2,4]triazole-3-thiol (B.1.32), 2-[2-chloro-4-(4-chlorophenoxy)phenyl]-1 -(1 ,2,4-triazol-1 -yl)pentan-2-ol (B.1 .33) , 1 -[4- (4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1 -cyclopropyl-2-(1 ,2,4-triazol-1 -yl)ethanol (B.1.34), 2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1 -(1 ,2,4-triazol-1 -yl)butan-2-ol (B.1.35), 2-[2-chloro-4-(4-chlorophenoxy)phenyl]-1 -(1 ,2,4-triazol-1 -yl)butan-2-ol (B.1.36), 2-[4-(4-chloro- phenoxy)-2-(trifluoromethyl)phenyl]-3-methyl-1 -(1 ,2,4-triazol-1 -yl)butan-2-ol (B.1 .37), 2-[4-(4- chlorophenoxy)-2-(trifluoromethyl)phenyl]-1 -(1 ,2,4-triazol-1 -yl)propan-2-ol (B.1 .38), 2-[2-chloro- 4-(4-chlorophenoxy)phenyl]-3-methyl-1 -(1 ,2,4-triazol-1 -yl)butan-2-ol (B.1 .39), 2-[4-(4- chlorophenoxy)-2-(trifluoromethyl)phenyl]-1 -(1 ,2,4-triazol-1 -yl)pentan-2-ol (B.1.40), 2-[4-(4- fluorophenoxy)-2-(trifluoromethyl)phenyl]-1 -(1 ,2,4-triazol-1 -yl)propan-2-ol (B.1 .41 ), 2-[2-chloro-

4- (4-chlorophenoxy)phenyl]-1 -(1 ,2,4-triazol-1 -yl)pent-3-yn-2-ol (B.1.51 ); imidazoles: imazalil (B.1.42), pefurazoate (B.1.43), prochloraz (B.1.44), triflumizol (B.1 .45); pyrimidines, pyridines and piperazines: fenarimol (B.1.46), nuarimol (B.1 .47), pyrifenox (B.1 .48), triforine (B.1 .49), [3- (4-chloro-2-fluoro-phenyl)-5-(2,4-difluorophenyl)isoxazol-4-yl]-(3-pyridyl)methanol (B.1 .50); - Delta14-reductase inhibitors: aldimorph (B.2.1 ), dodemorph (B.2.2), dodemorph-acetate (B.2.3), fenpropimorph (B.2.4), tridemorph (B.2.5), fenpropidin (B.2.6), piperalin (B.2.7), spirox- amine (B.2.8);

Inhibitors of 3-keto reductase: fenhexamid (B.3.1 );

C) Nucleic acid synthesis inhibitors

- phenylamides or acyl amino acid fungicides: benalaxyl (C.1.1 ), benalaxyl-M (C.1 .2), kiral- axyl (C.1.3), metalaxyl (C.1.4), metalaxyl-M (mefenoxam, C.1 .5), ofurace (C.1 .6), oxadixyl (C.1.7);

others: hymexazole (C.2.1 ), octhilinone (C.2.2), oxolinic acid (C.2.3), bupirimate (C.2.4),

5- fluorocytosine (C.2.5), 5-fluoro-2-(p-tolylmethoxy)pyrimidin-4-amine (C.2.6), 5-fluoro-2-(4- fluorophenylmethoxy)pyrimidin-4-amine (C.2.7);

D) Inhibitors of cell division and cytoskeleton

tubulin inhibitors, such as benzimidazoles, thiophanates: benomyl (D1 .1 ), carbendazim (D1 .2), fuberidazole (D1.3), thiabendazole (D1 .4), thiophanate-methyl (D1.5); triazolopyrim- idines: 5-chloro-7-(4-methylpiperidin-1 -yl)-6-(2,4,6-trifluorophenyl)-[1 ,2,4]tri- azolo[1 ,5-a]pyrimidine (D1 .6);

other cell division inhibitors: diethofencarb (D2.1 ), ethaboxam (D2.2), pencycuron (D2.3), fluopicolide (D2.4), zoxamide (D2.5), metrafenone (D2.6), pyriofenone (D2.7);

E) Inhibitors of amino acid and protein synthesis

- methionine synthesis inhibitors (anilino-pyrimidines): cyprodinil (E.1 .1 ), mepanipyrim (E.1.2), pyrimethanil (E.1 .3);

protein synthesis inhibitors: blasticidin-S (E.2.1 ), kasugamycin (E.2.2), kasugamycin hy- drochloride-hydrate (E.2.3), mildiomycin (E.2.4), streptomycin (E.2.5), oxytetracyclin (E.2.6), polyoxine (E.2.7), validamycin A (E.2.8);

F) Signal transduction inhibitors

MAP / histidine kinase inhibitors: fluoroimid (F.1 .1 ), iprodione (F.1 .2), procymidone (F.1 .3), vinclozolin (F.1 .4), fenpiclonil (F.1 .5), fludioxonil (F.1.6);

G protein inhibitors: quinoxyfen (F.2.1 );

G) Lipid and membrane synthesis inhibitors

- Phospholipid biosynthesis inhibitors: edifenphos (G.1.1 ), iprobenfos (G.1 .2), pyrazophos (G.1.3), isoprothiolane (G.1 .4);

lipid peroxidation: dicloran (G.2.1 ), quintozene (G.2.2), tecnazene (G.2.3), tolclofos-methyl (G.2.4), biphenyl (G.2.5), chloroneb (G.2.6), etridiazole (G.2.7);

phospholipid biosynthesis and cell wall deposition: dimethomorph (G.3.1 ), flumorph (G.3.2), mandipropamid (G.3.3), pyrimorph (G.3.4), benthiavalicarb (G.3.5), iprovalicarb (G.3.6), valifenalate (G.3.7) and N-(1 -(1 -(4-cyano-phenyl)ethanesulfonyl)-but-2-yl) carbamic acid-(4- fluorophenyl) ester (G.3.8);

compounds affecting cell membrane permeability and fatty acides: propamocarb (G.4.1 ); fatty acid amide hydrolase inhibitors: oxathiapiprolin (G.5.1 ), 2-{3-[2-(1 -{[3,5-bis(di- fluoromethyl-1 H-pyrazol-1 -yl]acetyl}piperidin-4-yl)-1 ,3-thiazol-4-yl]-4,5-dihydro-1 ,2-oxazol-5- yl}phenyl methanesulfonate (G.5.2), 2-{3-[2-(1 -{[3, 5-bis(difluoromethyl)-1 H-pyrazol-1 - yl]acetyl}piperidin-4-yl) 1 ,3-thiazol-4-yl]-4,5-dihydro-1 ,2-oxazol-5-yl}-3-chlorophenyl methanesulfonate (G.5.3);

H) Inhibitors with Multi Site Action

- inorganic active substances: Bordeaux mixture (H.1.1 ), copper acetate (H.1 .2), copper hydroxide (H.1 .3), copper oxychloride (H.1 .4), basic copper sulfate (H.1.5), sulfur (H.1 .6);

thio- and dithiocarbamates: ferbam (H.2.1 ), mancozeb (H.2.2), maneb (H.2.3), metam (H.2.4), metiram (H.2.5), propineb (H.2.6), thiram (H.2.7), zineb (H.2.8), ziram (H.2.9);

organochlorine compounds (e. g. phthalimides, sulfamides, chloronitriles): anilazine (H.3.1 ), chlorothalonil (H.3.2), captafol (H.3.3), captan (H.3.4), folpet (H.3.5), dichlofluanid (H.3.6), dichlorophen (H.3.7), hexachlorobenzene (H.3.8), pentachlorphenole (H.3.9) and its salts, phthalide (H.3.10), tolylfluanid (H.3.1 1 ), N-(4-chloro-2-nitro-phenyl)-N-ethyl-4-methyl- benzenesulfonamide (H.3.12);

guanidines and others: guanidine (H.4.1 ), dodine (H.4.2), dodine free base (H.4.3), guazatine (H.4.4), guazatine-acetate (H.4.5), iminoctadine (H.4.6), iminoctadine-triacetate (H.4.7), iminoctadine-tris(albesilate) (H.4.8), dithianon (H.4.9), 2,6-dimethyl-1 H,5H- [1 ,4]dithiino[2,3-c:5,6-c']dipyrrole-1 ,3,5,7(2H,6H)-tetraone (H.4.10);

I) Cell wall synthesis inhibitors

inhibitors of glucan synthesis: validamycin (1.1.1 ), polyoxin B (1.1 .2); melanin synthesis inhibitors: pyroquilon (1.2.1 ), tricyclazole (1.2.2), carpropamid (1.2.3), di- cyclomet (I.2.4), fenoxanil (I.2.5);

J) Plant defence inducers

acibenzolar-S-methyl (J.1.1 ), probenazole (J.1 .2), isotianil (J.1 .3), tiadinil (J.1 .4), prohexa- dione-calcium (J.1.5); phosphonates: fosetyl (J.1 .6), fosetyl-aluminum (J.1.7), phosphorous acid and its salts (J.1.8), potassium or sodium bicarbonate (J.1 .9);

K) Unknown mode of action

bronopol (K.1.1 ), chinomethionat (K.1.2), cyflufenamid (K.1 .3), cymoxanil (K.1.4), dazomet (K.1.5), debacarb (K.1.6), diclomezine (K.1 .7), difenzoquat (K.1 .8), difenzoquat-methylsulfate (K.1.9), diphenylamin (K.1 .10), fenpyrazamine (K.1.1 1 ), flumetover (K.1.12), flusulfamide (K.1.13), flutianil (K.1.14), methasulfocarb (K.1 .15), nitrapyrin (K.1 .16), nitrothal-isopropyl (K.1.18), oxathiapiprolin (K.1 .19), tolprocarb (K.1 .20), oxin-copper (K.1 .21 ), proquinazid

(K.1.22), tebufloquin (K.1.23), tecloftalam (K.1 .24), triazoxide (K.1 .25), 2-butoxy-6-iodo- 3-propylchromen-4-one (K.1.26), 2-[3,5-bis(difluoromethyl)-1 H-pyrazol-1 -yl]-1 -[4-(4-{5-[2-(prop- 2-yn-1 -yloxy)phenyl]-4,5-dihydro-1 ,2-oxazol-3-yl}-1 ,3-thiazol-2-yl)piperidin-1 -yl]ethanone (K.1.27), 2-[3,5-bis(difluoromethyl)-1 H-pyrazol-1 -yl]-1 -[4-(4-{5-[2-fluoro-6-(prop-2-yn-1 -yl- oxy)phenyl]-4,5-dihydro-1 ,2-oxazol-3-yl}-1 ,3-thiazol-2-yl)piperidin-1 -yl]ethanone (K.1 .28), 2-[3,5- bis(difluoromethyl)-1 H-pyrazol-1 -yl]-1 -[4-(4-{5-[2-chloro-6-(prop-2-yn-1 -yloxy)phenyl]-4,5- dihydro-1 ,2-oxazol-3-yl}-1 ,3-thiazol-2-yl)piperidin-1 -yl]ethanone (K.1.29), N-(cyclo- propylmethoxyimino-(6-difluoro-methoxy-2,3-difluoro-phenyl)-methyl)-2-phenyl acetamide (K.1.30), N'-(4-(4-chloro-3-trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N-methyl formamidine (K.1.31 ), N'-(4-(4-fluoro-3-trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N- methyl formamidine (K.1.32), N'-(2-methyl-5-trifluoromethyl-4-(3-trimethylsilanyl-propoxy)- phenyl)-N-ethyl-N-methyl formamidine (K.1.33), N'-(5-difluoromethyl-2-methyl-4-(3-tri- methylsilanyl-propoxy)-phenyl)-N-ethyl-N-methyl formamidine (K.1.34), methoxy-acetic acid 6- tert-butyl-8-fluoro-2,3-dimethyl-quinolin-4-yl ester (K.1 .35), 3-[5-(4-methylphenyl)-2,3-dimethyl- isoxazolidin-3-yl]-pyridine (K.1 .36), 3-[5-(4-chloro-phenyl)-2,3-dimethyl-isoxazolidin-3-yl]- pyridine (pyrisoxazole) (K.1.37), N-(6-methoxy-pyridin-3-yl) cyclopropanecarboxylic acid amide (K.1.38), 5-chloro-1 -(4,6-dimethoxy-pyrimidin-2-yl)-2-methyl-1 H-benzoimidazole (K.1 .39), 2-(4- chloro-phenyl)-N-[4-(3,4-dimethoxy-phenyl)-isoxazol-5-yl]-2-prop-2-ynyloxy-acetamide, ethyl (Z)-3-amino-2-cyano-3-phenyl-prop-2-enoate (K.1.40), picarbutrazox (K.1.41 ), pentyl N-[6-[[(Z)- [(1 -methyltetrazol-5-yl)-phenyl-methylene]amino]oxymethyl]-2-pyridyl]carbamate (K.1 .42), 2-[2- [(7,8-difluoro-2-methyl-3-quinolyl)oxy]-6-fluoro-phenyl]propan-2-ol (K.1 .43), 2-[2-fluoro-6-[(8- fluoro-2-methyl-3-quinolyl)oxy]phen-yl]propan-2-ol (K.1 .44), 3-(5-fluoro-3,3,4,4-tetramethyl-3,4- dihydroisoquinolin-1 -yl)quinoline (K.1 .45), 3-(4,4-difluoro-3,3-dimethyl-3,4-dihydroisoquinolin-1 - yl)quinoline (K.1.46), 3-(4,4,5-trifluoro-3,3-dimethyl-3,4-dihydroisoquinolin-1 -yl)quinoline

(K.1.47), 9-fluoro-2,2-dimethyl-5-(3-quinolyl)-3H-1 ,4-benzoxazepine (K.1 .48).

The fungicides described by common names, their preparation and their activity e.g. against harmful fungi is known (cf.: http://www.alanwood.net/pesticides/); these substances are com- mercially available.

The fungicides described by lUPAC nomenclature, their preparation and their pesticidal activity is also known (cf. Can. J. Plant Sci. 48(6), 587-94, 1968; EP-A 141 317; EP-A 152 031 ; EP-A 226 917; EP-A 243 970; EP-A 256 503; EP-A 428 941 ; EP-A 532 022; EP-A 1 028 125; EP-A 1 035 122; EP-A 1 201 648; EP-A 1 122 244, JP 2002316902; DE 19650197; DE 10021412; DE 102005009458; US 3,296,272; US 3,325,503; WO 98/46608; WO 99/14187; WO 99/24413; WO 99/27783; WO 00/29404; WO 00/46148; WO 00/65913; WO 01/54501 ; WO 01/56358; WO 02/22583; WO 02/40431 ; WO 03/10149; WO 03/1 1853; WO 03/14103; WO 03/16286; WO 03/53145; WO 03/61388; WO 03/66609; WO 03/74491 ; WO 04/49804; WO 04/83193; WO 05/120234; WO 05/123689; WO 05/123690; WO 05/63721 ; WO 05/87772; WO 05/87773; WO 06/15866; WO 06/87325; WO 06/87343; WO 07/82098; WO 07/90624, WO 1 1/028657, WO2012/168188, WO 2007/006670, WO 201 1/77514; WO13/047749, WO 10/069882, WO 13/047441 , WO 03/16303, WO 09/90181 , WO 13/007767, WO 13/010862, WO 13/127704, WO 13/024009, WO 13/024010 and WO 13/047441 , WO 13/162072, WO 13/092224, WO 1 1/135833).

With regard to the use of a mixture in the methods according to the present invention, the following mixtures of fungicidal compounds with afidopyropen are preferred:

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from the strobilurines, particularly selected from the strobilurines

azoxystrobin, dimoxystrobin, enestroburin, fluoxastrobin, kresoxim-methyl, metominostrobin, orysastrobin, picoxystrobin, pyraclostrobin, pyribencarb and trifloxystrobin.

Especially preferred is the combination with pyraclostrobin.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from the carboxamides, particularly selected from bixafen, boscalid, fluopy- ram, fluxapyroxad, isopyrazam, penflufen, penthiopyrad, sedaxane, metalaxyl and ofurace. Especially preferred is the combination with fluxapyroxad.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from dimethomorph, flumorph, fluopicolid (picobenzamid) or zoxamide.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from carpropamid or mandipropamid.

Preference is given to mixtures comprising a afidopyropen and as fungicidal compound II a fungicide selected from the group of azoles, particularly selected from the azoles cyprocona- zole, difenoconazole, epoxiconazole, fluquinconazole, flusilazole, flutriafol, hymexazole, ipcona- zole, imazalil, metconazole, myclobutanil, penconazole, propiconazole, prothioconazole, triadi- mefon, triadimenol, tebuconazole, tetraconazole, triticonazole, prochloraz, cyazofamid, ethaboxam and 2-[2-chloro-4-(4-chlorophenoxy)phenyl]-1 -(1 ,2,4-triazol-1 -yl)pentan-2-ol, 1 -[4-(4- chlorophenoxy)-2-(trifluoromethyl)phenyl]-1 -cyclopropyl-2-(1 ,2,4-triazol-1 -yl)ethanol, 2-[4-(4- chlorophenoxy)-2-(trifluoromethyl)phenyl]-1 -(1 ,2,4-triazol-1 -yl)butan-2-ol, 2-[2-chloro-4-(4- chlorophenoxy)phenyl]-1 -(1 ,2,4-triazol-1 -yl)butan-2-ol, 2-[4-(4-chlorophenoxy)-2-

(trifluoromethyl)phenyl]-3-methyl-1 -(1 ,2,4-triazol-1-yl)butan-2-ol, 2-[4-(4-chlorophenoxy)-2- (trifluoromethyl)phenyl]-1 -(1 ,2,4-triazol-1 -yl)propan-2-ol, 2-[2-chloro-4-(4-chlorophenoxy)phenyl]- 3-methyl-1 -(1 ,2,4-triazol-1 -yl)butan-2-ol, 2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1 - (1 ,2,4-triazol-1 -yl)pentan-2-ol, 2-[4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl]-1 -(1 ,2,4-triazol- 1 -yl)propan-2-ol.

Preference is also given to mixtures comprising afidopyropen and fluazinam.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from cyprodinil, fenarimol, mepanipyrim or pyrimethanil.

Preference is also given to mixtures comprising afidopyropen and triforine.

Preference is also given to mixtures comprising afidopyropen and a fungicidal compound II se- lected from the group of morpholines, in particular of dodemorph, fenpropimorph or tridemorph

Preference is also given to mixtures comprising afidopyropen and silthiofam.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from iprodione or vinclozolin.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from famoxadone, fenamidone, probenazole or proquinazid. Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from acibenzolar-S-methyl, captafol, folpet, fenoxanil, quinoxyfen or ametoc- tradin.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from mancozeb, metiram, propineb or thiram.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from iprovalicarb, benthiavalicarb or propamocarb. Preference is also given to mixtures comprising afidopyropen and dithianon.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from the fentin salts, such as fentin acetate. Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from fosetyl, fosetyl-aluminium, phosphorous acid and salts.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from chlorthalonil and dichlofluanid.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from copper acetate, copper hydroxide, copper oxychloride, copper sulfate and sulfur. Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from cymoxanil, metrafenone and spiroxamine.

Preference is also given to mixtures comprising afidopyropen and as fungicidal compound II a fungicide selected from thiophanate-methyl, benomyl, carbendazim and thiabendazol.

Surprisingly the use of afidopyropen alone or in mixtures (as defined herein above) in methods of applications on cultivated plants may display an unexpected improved efficacy up tosynergis- tic effects between the trait of the cultivated plant and the applied afidopyropen and its mixtures.

In a particular preferred embodiment, the mixtures comprise as an additional component which is the compound, against which the cultivated plant is resistant.

Ratios:

In general, the ratios by weight for the respective mixtures comprising the insecticidal compound I and compound II are from 1 :500 to 500:1 , preferably from 1 :100 to 100:1 , more preferably from 1 :25 to 25:1.

We have found that the application of compound of formula I and their mixtures (in case of the mixtures, the simultaneous, that is joint or separate, application of the compound I and compound II or successive application of the compound I and compound II) on cultivated plants allows enhanced control of animal pests, compared to the control rates that are possible by application of compound of formula I and their mixtures on non-cultivated plants. Formulations

The invention also relates to agrochemical compositions comprising an auxiliary and at least one compound of the present invention or a mixture thereof.

An agrochemical composition comprises a pesticidally effective amount of a compound of the present invention or a mixture thereof. The term "pesticidally effective amount" is defined below. The compounds of the present invention or the mixtures thereof can be converted into customary types of agro-chemical compositions, e. g. solutions, emulsions, suspensions, dusts, powders, pastes, granules, pressings, capsules, and mixtures thereof. Examples for composition types are suspensions (e.g. SC, OD, FS), emulsifiable concentrates (e.g. EC), emulsions (e.g. EW, EO, ES, ME), capsules (e.g. CS, ZC), pastes, pastilles, wettable powders or dusts (e.g. WP, SP, WS, DP, DS), pressings (e.g. BR, TB, DT), granules (e.g. WG, SG, GR, FG, GG, MG), insecticidal articles (e.g. LN), as well as gel formulations for the treatment of plant propagation materials such as seeds (e.g. GF). These and further compositions types are defined in the "Catalogue of pesticide formulation types and international coding system", Technical Monograph No. 2, 6th Ed. May 2008, CropLife International.

The compositions are prepared in a known manner, such as described by Mollet and Grube- mann, Formulation technology, Wiley VCH, Weinheim, 2001 ; or Knowles, New developments in crop protection product formulation, Agrow Reports DS243, T&F Informa, London, 2005.

Examples for suitable auxiliaries are solvents, liquid carriers, solid carriers or fillers, surfactants, dispersants, emulsifiers, wetters, adjuvants, solubilizers, penetration enhancers, protec- tive colloids, adhesion agents, thickeners, humectants, repellents, attractants, feeding stimulants, compatibilizers, bactericides, anti-freezing agents, anti-foaming agents, colorants, tackifi- ers and binders.

Suitable solvents and liquid carriers are water and organic solvents, such as mineral oil frac- tions of medium to high boiling point, e.g. kerosene, diesel oil; oils of vegetable or animal origin; aliphatic, cyclic and aromatic hydrocarbons, e. g. toluene, paraffin, tetrahydronaphthalene, alkylated naphthalenes; alcohols, e.g. ethanol, propanol, butanol, benzylalcohol, cyclo^hexanol; glycols; DMSO; ketones, e.g. cyclohexanone; esters, e.g. lactates, carbonates, fatty acid esters, gamma-butyrolactone; fatty acids; phosphonates; amines; amides, e.g. N-methylpyrrolidone, fatty acid dimethylamides; and mixtures thereof.

Suitable solid carriers or fillers are mineral earths, e.g. silicates, silica gels, talc, kaolins, limestone, lime, chalk, clays, dolomite, diatomaceous earth, bentonite, calcium sulfate, magnesium sulfate, magnesium oxide; polysaccharide powders, e.g. cellulose, starch; fertilizers, e.g. ammonium sulfate, ammonium phosphate, ammonium nitrate, ureas; products of vegetable origin, e.g. cereal meal, tree bark meal, wood meal, nutshell meal, and mixtures thereof.

Suitable surfactants are surface-active compounds, such as anionic, cationic, nonionic and amphoteric surfactants, block polymers, polyelectrolytes, and mixtures thereof. Such surfactants can be used as emusifier, dispersant, solubilizer, wetter, penetration enhancer, protective colloid, or adjuvant. Examples of surfactants are listed in McCutcheon's, Vol.1 : Emulsifiers & De- tergents, McCutcheon's Directories, Glen Rock, USA, 2008 (International Ed. or North American Ed.).

Suitable anionic surfactants are alkali, alkaline earth or ammonium salts of sulfonates, sulfates, phosphates, carboxylates, and mixtures thereof. Examples of sulfonates are alkylaryl- sulfonates, diphenylsulfonates, alpha-olefin sulfonates, lignine sulfonates, sulfonates of fatty acids and oils, sulfonates of ethoxylated alkylphenols, sulfonates of alkoxylated arylphenols, sulfonates of condensed naphthalenes, sulfonates of dodecyl- and tridecylbenzenes, sulfonates of naphthalenes and alkyhnaphthalenes, sulfosuccinates or sulfosuccinamates. Examples of sulfates are sulfates of fatty acids and oils, of ethoxylated alkylphenols, of alcohols, of ethoxylated alcohols, or of fatty acid esters. Examples of phosphates are phosphate esters. Exam- pies of carboxylates are alkyl carboxylates, and carboxylated alcohol or alkylphenol eth- oxylates.

Suitable nonionic surfactants are alkoxylates, N-subsituted fatty acid amides, amine oxides, esters, sugar-based surfactants, polymeric surfactants, and mixtures thereof. Examples of alkoxylates are compounds such as alcohols, alkylphenols, amines, amides, arylphenols, fatty acids or fatty acid esters which have been alkoxylated with 1 to 50 equivalents. Ethylene oxide and/or propylene oxide may be employed for the alkoxylation, preferably ethylene oxide. Examples of N-subsititued fatty acid amides are fatty acid glucamides or fatty acid alkanolamides. Examples of esters are fatty acid esters, glycerol esters or monoglycerides. Examples of sugar- based surfactants are sorbitans, ethoxylated sorbitans, sucrose and glucose esters or al- kylpolyglucosides. Examples of polymeric surfactants are homo- or copolymers of vinyl pyrroli- done, vinylalcohols, or vinylacetate.

Suitable cationic surfactants are quaternary surfactants, for example quaternary ammonium compounds with one or two hydrophobic groups, or salts of long-chain primary amines. Suitable amphoteric surfactants are alkylbetains and imidazolines. Suitable block polymers are block polymers of the A-B or A-B-A type comprising blocks of polyethylene oxide and polypropylene oxide, or of the A-B-C type comprising alkanol, polyethylene oxide and polypropylene oxide. Suitable polyelectrolytes are polyacids or polybases. Examples of polyacids are alkali salts of polyacrylic acid or polyacid comb polymers. Examples of polybases are polyvinylamines or pol- yethyleneamines.

Suitable adjuvants are compounds, which have a neglectable or even no pesticidal activity themselves, and which improve the biological performance of the compounds of the present invention on the target. Examples are surfactants, mineral or vegetable oils, and other auxi- laries. Further examples are listed by Knowles, Adjuvants and additives, Agrow Reports DS256, T&F Informa UK, 2006, chapter 5.

Suitable thickeners are polysaccharides (e.g. xanthan gum, carboxymethylcellulose), anorganic clays (organically modified or unmodified), polycarboxylates, and silicates.

Suitable bactericides are bronopol and isothiazolinone derivatives such as alkylisothiazoli- nones and benzisothiazolinones.

Suitable anti-freezing agents are ethylene glycol, propylene glycol, urea and glycerin.

Suitable anti-foaming agents are silicones, long chain alcohols, and salts of fatty acids.

Suitable colorants (e.g. in red, blue, or green) are pigments of low water solubility and water- soluble dyes. Examples are inorganic colorants (e.g. iron oxide, titan oxide, iron hexacyanofer- rate) and organic colorants (e.g. alizarin-, azo- and phthalocyanine colorants).

Suitable tackifiers or binders are polyvinylpyrrolidone, polyvinylacetates, polyvinyl alcohols, polyacrylates, biological or synthetic waxes, and cellulose ethers.

The agrochemical compositions generally comprise between 0.01 and 95%, preferably between 0.1 and 90%, and most preferably between 0.5 and 75%, by weight of active sub-stance. The active substances are employed in a purity of from 90% to 100%, preferably from 95% to 100% (according to NMR spectrum).

Various types of oils, wetters, adjuvants, fertilizer, or micronutrients, and other pesticides (e.g. herbicides, insecticides, fungicides, growth regulators, safeners) may be added to the active substances or the compositions cormprising them as premix or, if appropriate not until immediately prior to use (tank mix). These agents can be admixed with the compositions according to the invention in a weight ratio of 1 :100 to 100:1 , preferably 1 :10 to 10:1.

The user applies the composition according to the invention usually from a predosage de-vice, a knapsack sprayer, a spray tank, a spray plane, or an irrigation system. Usually, the agrochemical composition is made up with water, buffer, and/or further auxiliaries to the desired application concentration and the ready-to-use spray liquor or the agrochemical composition according to the invention is thus obtained. Usually, 20 to 2000 liters, preferably 50 to 400 liters, of the ready-to-use spray liquor are applied per hectare of agricultural useful area.

According to one embodiment, individual components of the composition according to the invention such as parts of a kit or parts of a binary or ternary mixture may be mixed by the user himself in a spray tank and further auxiliaries may be added, if appropriate.

In a further embodiment, either individual components of the composition according to the invention or partially premixed components, e. g. components comprising compounds of the present invention and/or mixing partners as defined further above, may be mixed by the user in a spray tank and further auxiliaries and additives may be added, if appropriate. In a further embodiment, either individual components of the composition according to the invention or partially premixed components, e. g. components comprising compounds of the present invention and/or mixing partners as defined further above, can be applied jointly (e.g. after tank mix) or consecutively.

Application methods

The compounds of the present invention are suitable for use in protecting crops, plants, plant propagation materials, such as seeds, or soil or water, in which the plants are growing, from attack or infestation by animal pests. Therefore, the present invention also relates to a plant protection method, which comprises contacting crops, plants, plant propagation materials, such as seeds, or soil or water, in which the plants are growing, to be protected from attack or infestation by animal pests, with a pesticidally effective amount of a compound of the present invention.

The compounds of the present invention are also suitable for use in combating or controlling animal pests. Therefore, the present invention also relates to a method of combating or controlling animal pests, which comprises contacting the animal pests, their habitat, breeding ground, or food supply, or the crops, plants, plant propagation materials, such as seeds, or soil, or the area, material or environment in which the animal pests are growing or may grow, with a pesticidally effective amount of a compound of the present invention.

The compounds of the present invention are effective through both contact and ingestion. Furthermore, the compounds of the present invention can be applied to any and all developmental stages, such as egg, larva, pupa, and adult.

The compounds of the present invention can be applied as such or in form of compositions comprising them as defined above. Furthermore, the compounds of the present invention can be applied together with a mixing partner as defined above or in form of compositions comprising said mixtures as defined above. The components of said mixture can be applied simultaneously, jointly or separately, or in succession, that is immediately one after another and thereby creating the mixture "in situ" on the desired location, e.g. the plant, the sequence, in the case of separate application, generally not having any effect on the result of the control measures. The application can be carried out both before and after the infestation of the crops, plants, plant propagation materials, such as seeds, soil, or the area, material or environment by the pests.

Suitable application methods include inter alia soil treatment, seed treatment, in furrow application, and foliar application. Soil treatment methods include drenching the soil, drip irrigation (drip application onto the soil), dipping roots, tubers or bulbs, or soil injection. Seed treatment techniques include seed dressing, seed coating, seed dusting, seed soaking, and seed pelleting. In furrow applications typically include the steps of making a furrow in cultivated land, seeding the furrow with seeds, applying the pesticidally active compound to the furrow, and closing the furrow. Foliar application refers to the application of the pesticidally active compound to plant foliage, e.g. through spray equipment. For foliar applications, it can be advantageous to modify the behavior of the pests by use of pheromones in combination with the compounds of the present invention. Suitable pheromones for specific crops and pests are known to a skilled person and publicly available from databases of pheromones and semiochemicals, such as http://www.pherobase.com. As used herein, the term "contacting" includes both direct contact (applying the compounds/compositions directly on the animal pest or plant - typically to the foliage, stem or roots of the plant) and indirect contact (applying the compounds/compositions to the locus, i.e. habitat, breeding ground, plant, seed, soil, area, material or environment in which a pest is growing or may grow, of the animal pest or plant).

The term "animal pest" includes arthropods, gastropods, and nematodes. Preferred animal pests according to the invention are arthropods, preferably insects and arachnids, in particular insects. Insects, which are of particular relevance for crops, are typically referred to as crop insect pests.

The term "crop" refers to both, growing and harvested crops.

The term "plant" includes cereals, e.g. durum and other wheat, rye, barley, triticale, oats, rice, or maize (fodder maize and sugar maize / sweet and field corn); beet, e.g. sugar beet or fodder beet; fruits, such as pomes, stone fruits or soft fruits, e.g. apples, pears, plums, peaches, nectarines, almonds, cherries, papayas, strawberries, raspberries, blackberries or gooseberries; leguminous plants, such as beans, lentils, peas, alfalfa or soybeans; oil plants, such as rape- seed (oilseed rape), turnip rape, mustard, olives, sunflowers, coconut, cocoa beans, castor oil plants, oil palms, ground nuts or soybeans; cucurbits, such as squashes, pumpkins, cucumber or melons; fiber plants, such as cotton, flax, hemp or jute; citrus fruit, such as oranges, lemons, grapefruits or mandarins; vegetables, such as eggplant, spinach, lettuce (e.g. iceberg lettuce), chicory, cabbage, asparagus, cabbages, carrots, onions, garlic, leeks, tomatoes, potatoes, cucurbits or sweet peppers; lauraceous plants, such as avocados, cinnamon or camphor; energy and raw material plants, such as corn, soybean, rapeseed, sugar cane or oil palm; tobacco; nuts, e.g. walnuts; pistachios; coffee; tea; bananas; vines (table grapes and grape juice grape vines); hop; sweet leaf (also called Stevia); natural rubber plants or ornamental and forestry plants, such as flowers (e.g. carnation, petunias, geranium/pelargoniums, pansies and impati- ens), shrubs, broad-leaved trees (e.g. poplar) or evergreens, e.g. conifers; eucalyptus; turf; lawn; grass such as grass for animal feed or ornamental uses. Preferred plants include potatoes sugar beets, tobacco, wheat, rye, barley, oats, rice, corn, cotton, soybeans, rapeseed, legumes, sunflowers, coffee or sugar cane; fruits; vines; ornamentals; or vegetables, such as cucumbers, tomatoes, beans or squashes.

The term "plant" is to be understood as including wild type plants and plants, which have been modified by either conventional breeding, or mutagenesis or genetic engineering, or by a combination thereof.

Plants, which have been modified by mutagenesis or genetic engineering, and are of particular commercial importance, include alfalfa, rapeseed (e.g. oilseed rape), bean, carnation, chicory, cotton, eggplant, eucalyptus, flax, lentil, maize, melon, papaya, petunia, plum, poplar, potato, rice, soybean, squash, sugar beet, sugarcane, sunflower, sweet pepper, tobacco, tomato, and cereals (e.g. wheat), in particular maize, soybean, cotton, wheat, and rice. In plants, which have been modified by mutagenesis or genetic engineering, one or more genes have been mutagen- ized or integrated into the genetic material of the plant. The one or more mutagenized or integrated genes are preferably selected from pat, epsps, crylAb, bar, cry1 Fa2, crylAc, cry34Ab1 , cry35AB1 , cry3A, cryF, cry1 F, mcry3a, cry2Ab2, cry3Bb1 , cry1A.105, dfr, barnase, vip3Aa20, barstar, als, bxn, bp40, asnl , and ppo5. The mutagenesis or integration of the one or more genes is performed in order to improve certain properties of the plant. Such properties, also known as traits, include abiotic stress tolerance, altered growth/yield, disease resistance, herbicide tolerance, insect resistance, modified product quality, and pollination control. Of these properties, herbicide tolerance, e.g. imidazolinone tolerance, glyphosate tolerance, or glufosinate tolerance, is of particular importance. Several plants have been rendered tolerant to herbicides by mutagenesis, for example Clearfield® oilseed rape being tolerant to imidazoli- nones, e.g. imazamox. Alternatively, genetic engineering methods have been used to render plants, such as soybean, cotton, corn, beets and oil seed rape, tolerant to herbicides, such as glyphosate and glufosinate, some of which are commercially available under the trade names RoundupReady® (glyphosate) and LibertyLink® (glufosinate). Furthermore, insect resistance is of importance, in particular lepidopteran insect resistance and coleopteran insect resistance. Insect resistance is typically achieved by modifying plants by integrating cry and/or vip genes, which were isolated from Bacillus thuringiensis (Bt), and code for the respective Bt toxins. Genetically modified plants with insect resistance are commercially available under trade names including WideStrike®, Bollgard®, Agrisure®, Herculex®, YieldGard®, Genuity®, and Intacta®. Plants may be modified by mutagenesis or genetic engineering either in terms of one property (singular traits) or in terms of a combination of properties (stacked traits). Stacked traits, e.g. the combination of herbicide tolerance and insect resistance, are of increasing importance. In general, all relevant modified plants in connection with singular or stacked traits as well as detailed information as to the mutagenized or integrated genes and the respective events are available from websites of the organizations "International Service for the Acquisition of Agri-biotech Applications (ISAAA)" (http://www.isaaa.org/gmapprovaldatabase) and "Center for Environmental Risk Assessment (CERA)" (http://cera-gmc.org/GMCropDatabase).

It has surprisingly been found that the pesticidal activity of the compounds of the present invention may be enhanced by the insecticidal trait of a modified plant. Furthermore, it has been found that the compounds of the present invention are suitable for preventing insects to become resistant to the insecticidal trait or for combating pests, which already have become resistant to the insecticidal trait of a modified plant. Moreover, the compounds of the present invention are suitable for combating pests, against which the insecticidal trait is not effective, so that a complementary insecticidal activity can advantageously be used.

The term "plant propagation material" refers to all the generative parts of the plant such as seeds and vegetative plant material such as cuttings and tubers (e.g. potatoes), which can be used for the multiplication of the plant. This includes seeds, roots, fruits, tubers, bulbs, rhizomes, shoots, sprouts and other parts of plants. Seedlings and young plants, which are to be transplanted after germination or after emergence from soil, may also be included. These plant propagation materials may be treated prophylactically with a plant protection compound either at or before planting or transplanting.

The term "seed" embraces seeds and plant propagules of all kinds including but not limited to true seeds, seed pieces, suckers, corms, bulbs, fruit, tubers, grains, cuttings, cut shoots and the like, and means in a preferred embodiment true seeds.

In general, "pesticidally effective amount" means the amount of active ingredient needed to achieve an observable effect on growth, including the effects of necrosis, death, retardation, prevention, and removal, destruction, or otherwise diminishing the occurrence and activity of the target organism. The pesticidally effective amount can vary for the various compounds/compositions used in the invention. A pesticidally effective amount of the compositions will also vary according to the prevailing conditions such as desired pesticidal effect and duration, weather, target species, locus, mode of application, and the like.

In the case of soil treatment, in furrow application or of application to the pests dwelling place or nest, the quantity of active ingredient ranges from 0.0001 to 500 g per 100 m², preferably from 0.001 to 20 g per 100 m².

For use in treating crop plants, e.g. by foliar application, the rate of application of the active ingredients of this invention may be in the range of 0.0001 g to 4000 g per hectare, e.g. from 1 g to 2 kg per hectare or from 1 g to 750 g per hectare, desirably from 1 g to 100 g per hectare, more desirably from 10 g to 50 g per hectare, e.g., 10 to 20 g per hectare, 20 to 30 g per hec- tare, 30 to 40 g per hectare, or 40 to 50 g per hectare.

The compounds of the present invention are particularly suitable for use in the treatment of seeds in order to protect the seeds from insect pests, in particular from soil-living insect pests, and the resulting seedling's roots and shoots against soil pests and foliar insects. The present invention therefore also relates to a method for the protection of seeds from insects, in particular from soil insects, and of the seedling's roots and shoots from insects, in particular from soil and foliar insects, said method comprising treating the seeds before sowing and/or after pregermina- tion with a compound of the present invention. The protection of the seedling's roots and shoots is preferred. More preferred is the protection of seedling's shoots from piercing and sucking in- sects, chewing insects and nematodes.

The term "seed treatment" comprises all suitable seed treatment techniques known in the art, such as seed dressing, seed coating, seed dusting, seed soaking, seed pelleting, and in-furrow application methods. Preferably, the seed treatment application of the active compound is carried out by spraying or by dusting the seeds before sowing of the plants and before emergence of the plants.

The present invention also comprises seeds coated with or containing the active compound. The term "coated with and/or containing" generally signifies that the active ingredient is for the most part on the surface of the propagation product at the time of application, although a greater or lesser part of the ingredient may penetrate into the propagation product, depending on the method of application. When the said propagation product is (re)planted, it may absorb the active ingredient.

Suitable seed is for example seed of cereals, root crops, oil crops, vegetables, spices, ornamentals, for example seed of durum and other wheat, barley, oats, rye, maize (fodder maize and sugar maize / sweet and field corn), soybeans, oil crops, crucifers, cotton, sunflowers, ba- nanas, rice, oilseed rape, turnip rape, sugarbeet, fodder beet, eggplants, potatoes, grass, lawn, turf, fodder grass, tomatoes, leeks, pumpkin/squash, cabbage, iceberg lettuce, pepper, cucumbers, melons, Brassica species, melons, beans, peas, garlic, onions, carrots, tuberous plants such as potatoes, sugar cane, tobacco, grapes, petunias, geranium/pelargoniums, pansies and impatiens.

In addition, the active compound may also be used for the treatment of seeds from plants, which have been modified by mutagenisis or genetic engineering, and which e.g. tolerate the action of herbicides or fungicides or insecticides. Such modified plants have been described in detail above. Conventional seed treatment formulations include for example flowable concentrates FS, solutions LS, suspoemulsions (SE), powders for dry treatment DS, water dispersible powders for slurry treatment WS, water-soluble powders SS and emulsion ES and EC and gel formulation GF. These formulations can be applied to the seed diluted or undiluted. Application to the seeds is carried out before sowing, either directly on the seeds or after having pregerminated the latter. Preferably, the formulations are applied such that germination is not included.

The active substance concentrations in ready-to-use formulations, which may be obtained after two-to-tenfold dilution, are preferably from 0.01 to 60% by weight, more preferably from 0.1 to 40 % by weight.

In a preferred embodiment a FS formulation is used for seed treatment. Typically, a FS formulation may comprise 1 -800 g/l of active ingredient, 1 -200 g/l Surfactant, 0 to 200 g/l antifreezing agent, 0 to 400 g/l of binder, 0 to 200 g/l of a pigment and up to 1 liter of a solvent, preferably water.

Especially preferred FS formulations of the compounds of the present invention for seed treatment usually comprise from 0.1 to 80% by weight (1 to 800 g/l) of the active ingredient, from 0.1 to 20 % by weight (1 to 200 g/l) of at least one surfactant, e.g. 0.05 to 5 % by weight of a wetter and from 0.5 to 15 % by weight of a dispersing agent, up to 20 % by weight, e.g. from 5 to 20 % of an anti-freeze agent, from 0 to 15 % by weight, e.g. 1 to 15 % by weight of a pigment and/or a dye, from 0 to 40 % by weight, e.g. 1 to 40 % by weight of a binder (sticker /adhesion agent), optionally up to 5 % by weight, e.g. from 0.1 to 5 % by weight of a thickener, optionally from 0.1 to 2 % of an anti-foam agent, and optionally a preservative such as a biocide, antioxidant or the like, e.g. in an amount from 0.01 to 1 % by weight and a filler/vehicle up to 100 % by weight.

In the treatment of seed, the application rates of the compounds of the invention are generally from 0.1 g to 10 kg per 100 kg of seed, preferably from 1 g to 5 kg per 100 kg of seed, more preferably from 1 g to 1000 g per 100 kg of seed and in particular from 1 g to 200 g per 100 kg of seed, e.g. from 1 g to 100 g or from 5 g to 100 g per 100 kg of seed.

The invention therefore also relates to seed comprising a compound of the present invention, or an agriculturally useful salt thereof, as defined herein. The amount of the compound of the present invention or the agriculturally useful salt thereof will in general vary from 0.1 g to 10 kg per 100 kg of seed, preferably from 1 g to 5 kg per 100 kg of seed, in particular from 1 g to 1000 g per 100 kg of seed. For specific crops such as lettuce the rate can be higher.

The compounds of the present invention may also be used for improving the health of a plant. Therefore, the present invention also relates to a method for improving plant health by treating a plant, plant propagation material and/or the locus where the plant is growing or is to grow with an effective and non-phytotoxic amount of a compound of the present invention.

As used herein "an effective and non-phytotoxic amount" means that the compound is used in a quantity which allows to obtain the desired effect but which does not give rise to any phytotox- ic symptom on the treated plant or on the plant grown from the treated propagule or treated soil.

The terms "plant" and "plant propagation material" are defined above.

"Plant health" is defined as a condition of the plant and/or its products which is determined by several aspects alone or in combination with each other such as yield (for example increased biomass and/or increased content of valuable ingredients), quality (for example improved con- tent or composition of certain ingredients or shelf life), plant vigour (for example improved plant growth and/or greener leaves ("greening effect"), tolerance to abiotic (for example drought) and/or biotic stress (for example disease) and production efficiency (for example, harvesting efficiency, processability).

The above identified indicators for the health condition of a plant may be interdependent and may result from each other. Each indicator is defined in the art and can be determined by methods known to a skilled person.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials, or at their locus of growth with the compound of formula I and its mixtures, wherein the plant is a plant, which express at least one insecticidal toxin, preferably a toxin from Bacillus species, more preferably from Bacillus thuringiensis.

In one embodiment, these are the insecticidal traits, against which the insects to be controlled are resistant in the methods of the first embodiment of the present invention.

In another embodiment, these are the traits, which have not yet been described in a trait combination for the the compound of formula (I) and its mixtures, accordingt to the second or third embodiment of the invention. Plant health and cultivated plants

In a preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of a cultivated plant, in particular the yield of a cultivated plant, by treating plant propagation material, preferably seeds with compounds of formula I and their mixtures.

The present invention also comprises plant propagation material, preferably seed, of a cultivated plant treated with compounds of formula I and their mixtures

In another preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of a cultivated plant, in particular the yield of a cultivated plant by treating the cultivated plant, part(s) of such plant or at its locus of growth with compounds of formula I and their mixtures.

The term cultivated plant(s) includes "modified plant(s)" and "transgenic plant(s)".

In one embodiment of the invention, the term "cultivated plants" refers to "modified plants". In one embodiment of the invention, the term "cultivated plants" refers to "transgenic plants". "Modified plants" are those which have been modified by conventional breeding techniques. The term "modification" means in relation to modified plants a change in the genome, epigenome, tran- scriptome or proteome of the modified plant, as compared to the control, wild type, mother or parent plant whereby the modification confers a trait (or more than one trait) or confers the in- crease of a trait (or more than one trait) as listed below.

The modification may result in the modified plant to be a different, for example a new plant variety than the parental plant.

"Transgenic plants" are those, which genetic material has been modified by the use of recombinant DNA techniques that under natural circumstances can not readily be obtained by cross breeding, mutations or natural recombination, whereby the modification confers a trait (or more than one trait) or confers the increase of a trait (or more than one trait) as listed below as compared to the wild-type plant.

In one embodiment, one or more genes have been integrated into the genetic material of a ge- netically modified plant in order to improve certain properties of the plant, preferably increase a trait as listed below as compared to the wild-type plant. Such genetic modifications also include but are not limited to targeted post-translational modification of protein(s), or to post- transcriptional modifications of oligo- or polypeptides e.g. by glycosylation or polymer additions such as prenylated, acetylated, phosphorylated or farnesylated moieties or PEG moieties.

In one embodiment under the term "modification" when referring to a transgenic plant or parts thereof is understood that the activity, expression level or amount of a gene product or the metabolite content is changed, e.g. increased or decreased, in a specific volume relative to a corresponding volume of a control, reference or wild-type plant or plant cell, including the de novo creation of the activity or expression.

In one embodiment the activity of a polypeptide is increased or generated by expression or overexpresion of the gene coding for said polypeptide which confers a trait or confers the increase of a trait as listed below as compared to the control plant. The term "expression" or "gene expression" means the transcription of a specific gene or specific genes or specific genet- ic construct. The term "expression" or "gene expression" in particular means the transcription of a gene or genes or genetic construct into structural RNA (rRNA, tRNA), regulatory RNA (e.g. miRNA, RNAi, RNAa) or mRNA with or without subsequent translation of the latter into a protein. In another embodiment the term "expression" or "gene expression" in particular means the transcription of a gene or genes or genetic construct into structural RNA (rRNA, tRNA) or mRNA with or without subsequent translation of the latter into a protein. In yet another embodiment it means the transcription of a gene or genes or genetic construct into mRNA.

The process includes transcription of DNA and processing of the resulting mRNA product. The term "increased expression" or "overexpression" as used herein means any form of expression that is additional to the original wild-type expression level.

The term "expression of a polypeptide" is understood in one embodiment to mean the level of said protein or polypeptide, preferably in an active form, in a cell or organism.

In one embodiment the activity of a polypeptide is decreased by decreased expression of the gene coding for said polypeptide which confers a trait or confers the increase of a trait as listed below as compared to the control plant. Reference herein to "decreased expression" or "reduc- tion or substantial elimination" of expression is taken to mean a decrease in endogenous gene expression and/or polypeptide levels and/or polypeptide activity relative to control plants. It comprises further reducing, repressing, decreasing or deleting of an expression product of a nucleic acid molecule.

The terms "reduction", "repression", "decrease" or "deletion" relate to a corresponding change of a property in an organism, a part of an organism such as a tissue, seed, root, tuber, fruit, leave, flower etc. or in a cell. Under "change of a property" it is understood that the activity, expression level or amount of a gene product or the metabolite content is changed in a specific volume or in a specific amount of protein relative to a corresponding volume or amount of protein of a control, reference or wild type. Preferably, the overall activity in the volume is reduced, decreased or deleted in cases if the reduction, decrease or deletion is related to the reduction, decrease or deletion of an activity of a gene product, independent whether the amount of gene product or the specific activity of the gene product or both is reduced, decreased or deleted or whether the amount, stability or translation efficacy of the nucleic acid sequence or gene encoding for the gene product is reduced, decreased or deleted.

The terms "reduction", "repression", "decrease" or "deletion" include the change of said property in only parts of the subject of the present invention, for example, the modification can be found in compartment of a cell, like an organelle, or in a part of a plant, like tissue, seed, root, leave, tuber, fruit, flower etc. but is not detectable if the overall subject, i.e. complete cell or plant, is tested. Preferably, the "reduction", "repression", "decrease" or "deletion" is found cellular, thus the term "reduction, decrease or deletion of an activity" or "reduction, decrease or deletion of a metabolite content" relates to the cellular reduction, decrease or deletion compared to the wild type cell. In addition the terms "reduction", "repression", "decrease" or "deletion" include the change of said property only during different growth phases of the organism used in the inventive process, for example the reduction, repression, decrease or deletion takes place only during the seed growth or during blooming. Furthermore the terms include a transitional reduction, decrease or deletion for example because the used method, e.g. the antisense, RNAi, snRNA, dsRNA, siRNA, miRNA, ta-siRNA, cosuppression molecule, or ribozyme, is not stable integrated in the genome of the organism or the reduction, decrease, repression or deletion is under control of a regulatory or inducible element, e.g. a chemical or otherwise inducible promoter, and has therefore only a transient effect.

Methods to achieve said reduction, decrease or deletion in an expression product are known in the art, for example from the international patent application WO 2008/034648, particularly in paragraphs [0020.1.1 .1 ], [0040.1 .1.1], [0040.2.1 .1] and [0041 .1 .1.1].

Reducing, repressing, decreasing or deleting of an expression product of a nucleic acid molecule in modified plants is known. Examples are canola i.e. double nill oilseed rape with reduced amounts of erucic acid and sinapins.

Such a decrease can also be achieved for example by the use of recombinant DNA technology, such as antisense or regulatory RNA (e.g. miRNA, RNAi, RNAa) or siRNA approaches. In particular RNAi, snRNA, dsRNA, siRNA, miRNA, ta-siRNA, cosuppression molecule, ribozyme, or antisense nucleic acid molecule, a nucleic acid molecule conferring the expression of a dominant-negative mutant of a protein or a nucleic acid construct capable to recombine with and silence, inactivate, repress or reduces the activity of an endogenous gene may be used to decrease the activity of a polypeptide in a transgenic plant or parts thereof or a plant cell thereof used in one embodiment of the methods of the invention. Examples of transgenic plants with reduced, repressed, decreased or deleted expression product of a nucleic acid molecule are Carica papaya (Papaya plants) with the event name X17-2 of the University of Florida, Prunus domestica (Plum) with the event name C5 of the United States Department of Agriculture - Agricultural Research Service, or those listed in rows T9-48 and T9-49 of table 9 below. Also known are plants with increased resistance to nematodes for example by reducing, repressing, decreasing or deleting of an expression product of a nucleic acid molecule, e.g. from the PCT pub- lication WO 2008/095886.

The reduction or substantial elimination is in increasing order of preference at least 10%, 20%, 30%, 40% or 50%, 60%, 70%, 80%, 85%, 90%, or 95%, 96%, 97%, 98%, 99% or more reduced compared to that of control plants. Reference herein to an "endogenous" gene not only refers to the gene in question as found in a plant in its natural form (i.e., without there being any human intervention), but also refers to that same gene (or a substantially homologous nucleic acid/gene) in an isolated form subsequently (re)introduced into a plant (a transgene). For example, a transgenic plant containing such a transgene may encounter a substantial reduction of the transgene expression and/or substantial reduction of expression of the endogenous gene. The terms "control" or "reference" are exchangeable and can be a cell or a part of a plant such as an organelle like a chloroplast or a tissue, in particular a plant, which was not modified or treated according to the herein described process according to the invention. Accordingly, the plant used as control or reference corresponds to the plant as much as possible and is as identical to the subject matter of the invention as possible. Thus, the control or reference is treated identically or as identical as possible, saying that only conditions or properties might be different which do not influence the quality of the tested property other than the treatment of the present invention.

It is possible that control or reference plants are wild-type plants. However, "control" or "reference" may refer to plants carrying at least one genetic modification, when the plants employed in the process of the present invention carry at least one genetic modification more than said control or reference plants. In one embodiment control or reference plants may be transgenic but differ from transgenic plants employed in the process of the present invention only by said modification contained in the transgenic plants employed in the process of the present invention.

The term "wild type" or "wild-type plants" refers to a plant without said genetic modification. These terms can refer to a cell or a part of a plant such as an organelle like a chloroplast or a tissue, in particular a plant, which lacks said genetic modification but is otherwise as identical as possible to the plants with at least one genetic modification employed in the present invention. In a particular embodiment the "wild-type" plant is not transgenic.

Preferably, the wild type is identically treated according to the herein described process according to the invention. The person skilled in the art will recognize if wild-type plants will not require certain treatments in advance to the process of the present invention, e.g. non-transgenic wild- type plants will not need selection for transgenic plants for example by treatment with a selecting agent such as a herbicide.

The control plant may also be a nullizygote of the plant to be assessed. The term "nullizygotes" refers to a plant that has undergone the same production process as a transgenic, yet has lost the once aquired genetic modification (e.g. due to mendelian segregation) as the corresponding transgenic. If the starting material of said production process is transgenic, then nullizygotes are also transgenic but lack the additional genetic modification introduced by the production pro- cess. In the process of the present invention the purpose of wild-type and nullizygotes is the same as the one for control and reference or parts thereof. All of these serve as controls in any comparison to provide evidence of the advantageous effect of the present invention.

Preferably, any comparison is carried out under analogous conditions. The term "analogous conditions" means that all conditions such as, for example, culture or growing conditions, soil, nutrient, water content of the soil, temperature, humidity or surrounding air or soil, assay condi- tions (such as buffer composition, temperature, substrates, pathogen strain, concentrations and the like) are kept identical between the experiments to be compared. The person skilled in the art will recognize if wild-type, control or reference plants will not require certain treatments in advance to the process of the present invention, e.g. non-transgenic wild-type plants will not need selection for transgenic plants for example by treatment with herbicide.

In case that the conditions are not analogous the results can be normalized or standardized based on the control.

The "reference", "control", or "wild type" is preferably a plant, which was not modified or treated according to the herein described process of the invention and is in any other property as similar to a plant, employed in the process of the present invention of the invention as possible. The reference, control or wild type is in its genome, transcriptome, proteome or metabolome as similar as possible to a plant, employed in the process of the present invention of the present invention. Preferably, the term "reference-" "control-" or "wild-type-" plant, relates to a plant, which is nearly genetically identical to the organelle, cell, tissue or organism, in particular plant, of the present invention or a part thereof preferably 90% or more, e.g. 95%, more preferred are 98%, even more preferred are 99,00%, in particular 99,10%, 99,30%, 99,50%, 99,70%, 99,90%, 99,99%, 99,999% or more. Most preferable the "reference", "control", or "wild type" is a plant, which is genetically identical to the plant, cell, a tissue or organelle used according to the process of the invention except that the responsible or activity conferring nucleic acid molecules or the gene product encoded by them have been amended, manipulated, exchanged or introduced in the organelle, cell, tissue, plant, employed in the process of the present invention.

Preferably, the reference and the subject matter of the invention are compared after standardization and normalization, e.g. to the amount of total RNA, DNA, or protein or activity or expression of reference genes, like housekeeping genes, such as ubiquitin, actin or ribosomal proteins.

The genetic modification carried in the organelle, cell, tissue, in particular plant used in the process of the present invention is in one embodiment stable e.g. due to a stable transgenic integration or to a stable mutation in the corresponding endogenous gene or to a modulation of the expression or of the behaviour of a gene, or transient, e.g. due to an transient transformation or temporary addition of a modulator such as an agonist or antagonist or inducible, e.g. after trans- formation with a inducible construct carrying a nucleic acid molecule under control of a inducible promoter and adding the inducer, e.g. tetracycline.

In one embodiment preferred plants, from which "modified plants" and/or "transgenic plants" are be selected from the group consisting of cereals, such as maize (corn), wheat, barley sorghum, rice, rye, millet, triticale, oat, pseudocereals (such as buckwheat and quinoa), alfalfa, apples, banana, beet, broccoli, Brussels sprouts, cabbage, canola (rapeseed), carrot, cauliflower, cherries, chickpea, Chinese cabbage, Chinese mustard, collard, cotton, cranberries, creeping bent- grass, cucumber, eggplant, flax, grape, grapefruit, kale, kiwi, kohlrabi, melon, mizuna, mustard, papaya, peanut, pears, pepper, persimmons, pigeonpea, pineapple, plum, potato, raspberry, rutabaga, soybean, squash, strawberries, sugar beet, sugarcane, sunflower, sweet corn, tobacco, tomato, turnip, walnut, watermelon and winter squash,

more preferably from the group consisting of alfalfa, canola (rapeseed), cotton, rice, maize, cer- als (such as wheat, barley, rye, oat), soybean, fruits and vegetables (such as potato, tomato, melon, papaya), pome fruits (such as apple and pear), vine, sugarbeet, sugarcane, rape, citrus fruits (such as citron, lime, orange, pomelo, grapefruit, and mandarin) and stone fruits (such as cherry, apricot and peach), most preferably from cotton, rice, maize, cerals (such as wheat, bar- ley, rye, oat), sorghum, squash, soybean, potato, vine, pome fruits (such as apple), citrus fruits (such as citron and orange), sugarbeet, sugarcane, rape, oilseed rape and tomatoes,, utmost preferably from cotton, rice, maize, wheat, barley, rye, oat, soybean, potato, vine, apple, pear, citron and orange. In another embodiment of the invention the cultivated plant is a gymnosperm plant, especially a spruce, pine or fir.

In one embodiment of the invention, the cultivated plants to be treated according to the methods of the present invention are considered "row crops".

A "row crop" is considered to be a crop that can be planted in rows wide enough to allow it to be tilled or otherwise cultivated by agricultural machinery, machinery tailored for the seasonal activities of row crops. In general, such crops are sown by drilling rather than broadcasting.

Examples of row crops include among others cotton, maize (corn) or soybeans.

In another embodiment of the invention, the cultivated plants to be treated according to the methods of the present invention are considered "specialty crops".

"Specialty crops" are considered in general defined as fruits and vegetables, tree nuts, dried fruits and horticulture and nursery crops, including floriculture. Specialty crops may range from garlic, ginger and chilies, to tomatoes and apples, to floriculture and organic agronomic crops. In order to illustrate the varity of individual plants and crops which are categorized as speciality crops, following non-limiting examples may be further listed, such as basil, basmati rice, buckwheat, chufa, hops, longan, lychee, mango, pineapple, rosemary, starfruit or tomatillos.

The cultivated plants are plants, which comprise at least one trait. The term "trait" refers to a property, which is present in the plant either by genetic engineering or by conventional breeding techniques. Each trait has to be assessed in relation to its respective control. Examples of traits are:

• herbicide tolerance,

• insect resistance by expression of bacterial toxins,

• fungal resistance or viral resistance or bacterial resistance,

· antibiotic resistance,

• stress tolerance,

• maturation alteration, • content modification of chemicals present in the cultivated plant, preferably increasing the content of fine chemicals advantageous for applications in the field of the food and/or feed industry, the cosmetics industry and/or the pharmaceutical industry,

• modified nutrient uptake, preferably an increased nutrient use efficiency and/or resistance to conditions of nutrient deficiency,

• improved fiber quality,

• plant vigor,

• modified colour,

• fertility restoration,

and male sterility.

Principally, cultivated plants may also comprise combinations of the aforementioned traits, e.g. they may be tolerant to the action of herbicides and express bacertial toxins. Principally, all cultivated plants may also provide combinations of the aforementioned properties, e.g. they may be tolerant to the action of herbicides and express bacertial toxins.

Tolerance to herbicides can be obtained by creating insensitivity at the site of action of the herbicide by expression of a target enzyme which is resistant to herbicide; rapid metabolism (conju- gation or degradation) of the herbicide by expression of enzymes which inactivate herbicide; or poor uptake and translocation of the herbicide. Examples are the expression of enzymes which are tolerant to the herbicide in comparison to wild type enzymes, such as the expression of 5- enolpyruvylshikimate-3-phosphate synthase (EPSPS), which is tolerant to glyphosate (see e.g. Heck et.al, Crop Sci. 45, 2005, 329-339; Funke et.al, PNAS 103, 2006, 13010-13015; US 5188642, US 4940835, US 5633435, US 5804425, US 5627061 ), the expression of glutamine synthase which is tolerant to glufosinate and bialaphos (see e.g. US 5646024, US 5561236) and DNA constructs coding for dicamba-degrading enzymes (see e.g. US 7105724). Gene constructs can be obtained, for example, from micro-organism or plants, which are tolerant to said herbicides, such as the Agrobacterium strain CP4 EPSPS which is resistant to glyphosate; Streptomyces bacteria which are resistance to glufosinate; Arabidopsis, Daucus carota, Pseu- domonoas spp. or Zea mais with chimeric gene sequences coding for HDDP (see e.g. WO 1996/38567, WO 2004/55191 ); Arabidopsis thaliana which is resistant to protox inhibitors (see e.g. US 2002/0073443).

Tolerance to glyphosate can also be achieved by any one of the genes 2mepsps, epsps, gat4601 , goxv247 or mepsps. Tolerance to glufosinate can be achieved by any one of the genes bar, pat or pat(syn).

Preferaby, the herbicide tolerant plant can be selected from cereals such as wheat, barley, rye, oat; canola, sorghum, soybean, rice, oil seed rape, sugar beet, sugarcane, grapes, lentils, sun- flowers, alfalfa, pome fruits; stone fruits; peanuts; coffee; tea; strawberries; turf; vegetables, such as tomatoes, potatoes, cucurbits and lettuce, more preferably, the plant is selected from soybean, maize (corn), rice, cotton, oilseed rape in particular canola, tomatoes, potatoes, sugarcane, vine, apple, pear, citron, orange and cereals such as wheat, barley, rye and oat. More preferably, the cultivated plant is selected from the group consisting of Gossypium hirsutum L. (cotton), Zea mays L. (maize), Glycine max L. (soybean), Triticum aestivum (wheat), and Oryza sativa L. (rice), preferably from the group consisting of Gossypium hirsutum L. (cotton), Zea mays L. (maize) and Glycine max L. (soybean). Particularly preferably, the cultivated plant is Glycine max L. (soybean).

Examples of commercial available transgenic plants with tolerance to herbicides, are the corn varieties "Roundup Ready Corn", "Roundup Ready 2" (Monsanto), "Agrisure GT", "Agrisure GT/CB/LL", "Agrisure GT/RW",„Agrisure 3000GT" (Syngenta), "YieldGard VT Rootworm/RR2" and "YieldGard VT Triple" (Monsanto) with tolerance to glyphosate; the corn varieties "Liberty Link" (Bayer), "Herculex I", "Herculex RW", "Herculex Xtra"(Dow, Pioneer), "Agrisure GT/CB/LL" and "Agrisure CB/LL/RW" (Syngenta) with tolerance to glufosinate; the soybean varieties "Roundup Ready Soybean" (Monsanto) and "Optimum GAT" (DuPont, Pioneer) with tolerance to glyphosate; the cotton varieties "Roundup Ready Cotton" and "Roundup Ready Flex" (Monsanto) with tolerance to glyphosate; the cotton variety "FiberMax Liberty Link" (Bayer) with tol- erance to glufosinate; the cotton variety "BXN" (Calgene) with tolerance to bromoxynil; the canola varieties ..Navigator" und ..Compass" (Rhone-Poulenc) with bromoxynil tolerance; the canola varierty"Roundup Ready Canola" (Monsanto) with glyphosate tolerance; the canola variety "InVigor" (Bayer) with glufosinate tolerance; the rice variety "Liberty Link Rice" (Bayer) with glulfosinate tolerance and the alfalfa variety "Roundup Ready Alfalfa" with glyphosate tolerance. Further transgenic plants with herbicide tolerance are commonly known, for instance alfalfa, apple, eucalyptus, flax, grape, lentils, oil seed rape, peas, potato, rice, sugar beet, sunflower, tobacco, tomatom turf grass and wheat with tolerance to glyphosate (see e.g. US 5188642, US 4940835, US 5633435, US 5804425, US 5627061 ); beans, soybean, cotton, peas, potato, sunflower, tomato, tobacco, corn, sorghum and sugarcane with tolerance to dicamba (see e.g. US 7105724 and US 5670454); pepper, apple, tomato, millet, sunflower, tobacco, potato, corn, cucumber, wheat and sorghum with tolerance to 2,4-D (see e.g. US 6153401 , US 6100446, WO 2005107437, US 5608147 and US 5670454); sugarbeet, potato, tomato and tobacco with tolerance to glufosinate (see e.g. US 5646024, US 5561236); canola, barley, cotton, lettuce, melon, millet, oats, potato, rice, rye, sorghum, soybean, sugarbeet, sunflower, tobacco, tomato and wheat with tolerance to acetolactate synthase (ALS) inhibiting herbicides, such as triazolopyrim- idine sulfonamides, sulfonylureas and imidazolinones (see e.g. US 5013659, WO 2006060634, US 4761373, US 5304732, US 621 1438, US 621 1439 and US 6222100); cereals, sugar cane, rice, corn, tobacco, soybean, cotton, rapeseed, sugar beet and potato with tolerance to HPPD inhibitor herbicides (see e.g. WO 2004/055191 , WO 199638567, WO 1997049816 and US 6791014); wheat, soybean, cotton, sugar beet, rape, rice, sorghum and sugar cane with tolerance to protoporphyrinogen oxidase (PPO) inhibitor herbicides (see e.g. US 2002/0073443, US 20080052798, Pest Management Science, 61 , 2005, 277-285). The methods of producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the publications mentioned above.

Plants, which are capable of synthesising one or more selectively acting bacterial toxins, comprise for example at least one toxin from toxin-producing bacteria, especially those of the genus Bacillus, in particular plants capable of synthesising one or more insecticidal proteins from Bacillus cereus or Bacillus popliae; or insecticidal proteins from Bacillus thuringiensis, such as del- ta.-endotoxins, e.g. CrylA(b), CrylA(c), CrylF, CrylF(a2), CryllA(b), CrylllA, CrylllB(bl ) or Cry9c, or vegetative insecticidal proteins (VIP), e.g. VIP1 , VIP2, VIP3 or VIP3A; or insecticidal proteins of bacteria colonising nematodes, for example Photorhabdus spp. or Xenorhabdus spp., such as Photorhabdus luminescens, Xenorhabdus nematophilus; toxins produced by animals, such as scorpion toxins, arachnid toxins, wasp toxins and other insect-specific neurotoxins; toxins produced by fungi, such as Streptomycetes toxins, plant lectins, such as pea lectins, barley lectins or snowdrop lectins; agglutinins; proteinase inhibitors, such as trypsine inhibitors, serine protease inhibitors, patatin, cystatin, papain inhibitors; ribosome-inactivating proteins (RIP), such as ricin, maize-RIP, abrin, luffin, saporin or bryodin; steroid metabolism enzymes, such as 3-hydroxysteroidoxidase, ecdysteroid-UDP-glycosyl-transferase, cholesterol oxidases, ecdy- sone inhibitors, HMG-COA-reductase, ion channel blockers, such as blockers of sodium or calcium channels, juvenile hormone esterase, diuretic hormone receptors, stilbene synthase, bibenzyl synthase, chitinases and glucanases. In one embodiment a plant is capable of producing a toxin, lectin or inhibitor if it contains at least one cell comprising a nucleic acid sequence encoding said toxin, lectin, inhibitor or inhibitor producing enzyme, and said nucleic acid sequence is transcribed and translated and if appropriate the resulting protein processed and/or secreted in a constitutive manner or subject to developmental, inducible or tissue-specific regulation.

In the context of the present invention there are to be understood delta. -endotoxins, for example CrylA(b), CrylA(c), CrylF, CrylF(a2), CryllA(b), CrylllA, CrylllB(bl ) or Cry9c, or vegetative insecticidal proteins (VIP), for example VIP1 , VIP2, VIP3 or VIP3A, expressly also hybrid toxins, truncated toxins and modified toxins. Hybrid toxins are produced recombinantly by a new combination of different domains of those proteins (see, for example, WO 02/15701 ). An example for a truncated toxin is a truncated CrylA(b), which is expressed in the Bt1 1 maize from Syngen- ta Seed SAS, as described below. In the case of modified toxins, one or more amino acids of the naturally occurring toxin are replaced. In such amino acid replacements, preferably non- naturally present protease recognition sequences are inserted into the toxin, such as, for example, in the case of CrylllA055, a cathepsin-D-recognition sequence is inserted into a CrylllA tox- in (see WO 2003/018810).

Examples of such toxins or transgenic plants capable of synthesising such toxins are disclosed, for example, in EP-A-0 374 753, WO 93/07278, WO 95/34656, EP-A-0 427 529, EP-A-451 878 and WO 2003/052073.

Examples of genes conferring resistance to coleopteran insects include cry34Ab1 , cry35 Ab1 , cry3A, cry3Bb1 , dvsnf7, and mcry3A.

Examples of genes conferring resistance to lepidopteran insects include cry1A, cry1A.105, crylAb, cry1Ab-Ac, crylAc, cryl C, cryl F, cry1 Fa2, cry2Ab2, cry2Ae, cry9c, mocryl F, pinll, vip3A(a), and vip3Aa20.

The processes for the preparation of such transgenic plants are generally known to the person skilled in the art and are described, for example, in the publications mentioned above. Cryl-type deoxyribonucleic acids and their preparation are known, for example, from WO 95/34656, EP-A- 0 367 474, EP-A-0 401 979 and WO 1990/13651.

The toxin contained in the transgenic plants imparts to the plants tolerance to harmful insects. Such insects can occur in any taxonomic group of insects, but are especially commonly found in the beetles (Coleoptera), two-winged insects (Diptera) and butterflies (Lepidoptera).

Preferably, the plant capable of expression of bacterial toxins is selected from cereals such as wheat, barley, rye, oat; canola, cotton, eggplant, lettuce, sorghum, soybean, rice, oil seed rape, sugar beet, sugarcane, grapes, lentils, sunflowers, alfalfa, pome fruits; stone fruits; peanuts; coffee; tea; strawberries; turf; vegetables, such as tomatoes, potatoes, cucurbits and lettuce, more preferably, the plant is selected from cotton, soybean, maize (corn), rice, tomatoes, potatoes, oilseed rape and cereals such as wheat, barley, rye and oat, most preferably from cotton, soybean, maize, vine, apple, pear, citron, orange and cereals such as wheat, barley, rye and oat. More preferably, the cultivated plant is selected from the group consisting of Gossypium hirsutum L. (cotton), Zea mays L (maize), Glycine max L (soybean), Triticum aestivum (wheat), and Oryza sativa L. (rice), preferably from the group consisting of Gossypium hirsutum L. (cotton), Zea mays L. (maize) and Glycine max L. (soybean). Particularly preferably, the cultivated plant is Glycine max L. (soybean).

Examples of commercial available transgenic plants capable of expression of bacterial toxins are the corn varieties "YieldGard corn rootworm" (Monsanto), "YieldGard VT" (Monsanto), "Her- culex RW" (Dow, Pioneer), "Herculex Rootworm" (Dow, Pioneer) and "Agrisure CRW" (Syngen- ta) with resistance against corn rootworm; the corn varieties "YieldGard corn borer" (Monsanto), „YieldGard VT Pro" (Monsanto), "Agrisure CB/LL" (Syngenta), "Agrisure 3000GT" (Syngenta), "Hercules I", "Hercules II" (Dow, Pioneer), "KnockOut" (Novartis),„NatureGard" (Mycogen) and „Starl_ink" (Aventis) with resistance against corn borer, the corn varieties„Herculex I" (Dow, Pioneer) and„Herculex Xtra" (Dow, Pioneer) with resistance against western bean cutworm, corn borer, black cutworm and fall armyworm; the corn variety "YieldGard Plus" (Monsanto) with resistance against corn borer and corn rootworm; the cotton variety "Bollgard I"" (Monsanto) with resistance against tobacco budworm; the cotton varieties "Bollgard II" (Monsanto),„WideStrike" (Dow) and„VipCot" (Syngenta) with resistance against tobacco budworm, cotton bollworm, fall armyworm, beet armyworm, cabbage looper, soybean lopper and pink bollworm; the potato varieties "NewLeaf", "NewLeaf Y" and "NewLeaf Plus" (Monsanto) with tobacco hornworm resistance and the eggplant varieties "Bt brinjal", "Dumaguete Long Purple", "Mara" with re- sistance against brinjal fruit and shoot borer, bruit borer and cotton bollworm (see e.g.

US5128130). Further transgenic plants with insect resistance are commonly known, such as yellow stemborer resistant rice (see e.g. Molecular Breeding, Volume 18, 2006, Number 1 ), lep- idopteran resistant lettuce (see e.g. US 5349124), resistant soybean (see e.g. US 7432421 ) and rice with resistance against Lepidopterans, such as rice stemborer, rice skipper, rice cutworm, rice caseworm, rice leaffolder and rice armyworm (see e.g. WO 2001021821 ). The methods of producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the publications mentioned above. Preferably, plants, which are capable of synthesising antipathogenic substances are selected from soybean, maize (corn), rice, tomatoes, potato, banana, papaya, tobacco, grape, plum and cereals such as wheat, barley, rye and oat, most preferably from soybean, maize (corn), rice, cotton, tomatoes, potato, banana, papaya, oil seed rape, vine, apple, pear, citron, orange and cereals such as wheat, barley, rye and oat.

Plants, which are capable of synthesising antipathogenic substances having a selective action are for example plants expressing the so-called "pathogenesis-related proteins" (PRPs, see e.g. EP-A-0 392 225) or so-called "antifungal proteins" (AFPs, see e.g. US 6864068). A wide range of antifungal proteins with activity against plant pathogenic fungi have been isolated from certain plant species and are common knowledge. Examples of such antipathogenic substances and transgenic plants capable of synthesising such antipathogenic substances are known, for example, from EP-A-0 392 225, WO 93/05153, WO 95/33818, and EP-A-0 353 191. Transgenic plants which are resistant against fungal, viral and bacterial pathogens are produced by intro- ducing plant resistance genes. Numerous resistant genes have been identified, isolated and were used to improve plant resistant, such as the N gene which was introduced into tobacco lines that are susceptible to Tobacco Mosaic Virus (TMV) in order to produce TMV-resistant tobacco plants (see e.g. US 5571706), the Prf gene, which was introduced into plants to obtain enhanced pathogen resistance (see e.g. WO 199802545) and the Rps2 gene from Arabidopsis thaliana, which was used to create resistance to bacterial pathogens including Pseudomonas syringae (see e.g. WO 199528423). Plants exhibiting systemic acquired resistance response were obtained by introducing a nucleic acid molecule encoding the TIR domain of the N gene (see e.g. US 6630618). Further examples of known resistance genes are the Xa21 gene, which has been introduced into a number of rice cultivars (see e.g. US 5952485, US 5977434, WO 1999/09151 , WO 1996/22375), the Rcg1 gene for colletotrichum resistance (see e.g. US 2006/225152), the prpl gene (see e.g. US 5859332, WO 2008/017706), the ppv-cp gene to introduce resistance against plum pox virus (see e.g. US PP15,154Ps), the P1 gene (see e.g. US5968828), genes such as Blb1 , Blb2, Blb3 and RB2 to introduce resistance against Phy- tophthora infestans \x\ potato (see e.g. US 7148397), the LRPKml gene (see e.g.

W01999064600), the P1 gene for potato virus Y resistance (see e.g. US 5968828), the HA5-1 gene (see e.g. US5877403 and US6046384), the PIP gene to indroduce a broad resistant to viruses, such as potato virus X (PVX), potato virus Y (PVY), potato leafroll virus (PLRV) (see e.g. EP 0707069) and genes such as Arabidopsis NI 16, ScaM4 and ScaM5 genes to obtain fungal resistance (see e.g. US 6706952 and EP 1018553). The methods of producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the publications mentioned above.

Antipathogenic substances which can be expressed by such transgenic plants include, for example, ion channel blockers, such as blockers for sodium and calcium channels, for example the viral KP1 , KP4 or KP6 toxins; stilbene synthases; bibenzyl synthases; chitinases; glu- canases; the so-called "pathogenesis-related proteins"" (PRPs; see e.g. EP-A-0 392 225); antipathogenic substances produced by microorganisms, for example peptide antibiotics or heterocyclic antibiotics (see e.g. WO 1995/33818) or protein or polypeptide factors involved in plant pathogen defense (so-called "plant disease resistance genes", as described in WO 2003/000906).

Antipathogenic substances produced by the plants are able to protect the plants against a varie- ty of pathogens, such as fungi, viruses and bacteria. Useful plants of elevated interest in connection with present invention are cereals, such as wheat, barley, rye and oat; soybean; maize; rice; alfalfa, cotton, sugar beet, sugarcane, tobacco , potato, banana, oil seed rape; pome fruits; stone fruits; peanuts; coffee; tea; strawberries; turf; vines and vegetables, such as tomatoes, potatoes, cucurbits, papaya, melon, lenses and lettuce, more preferably selected from soybean, maize (corn), alfalfa, cotton, potato, banana, papaya, rice, tomatoes and cereals such as wheat, barley, rye and oat, most preferably from soybean, maize (corn), rice, cotton, potato, tomato, oilseed rape, vine, apple, pear, citron, orange and cereals such as wheat, barley, rye and oat.

Transgenic plants with resistance against fungal pathogens, are, for examples, soybeans with resistance against Asian soybean rust (see e.g. WO 2008/017706); plants such as alfalfa, corn, cotton, sugar beet, oileed, rape, tomato, soybean, wheat, potato and tobacco with resistance against Phytophtora infestans (see e.g. US5859332, US 7148397, EP 1334979); corn with resistance against leaf blights, ear rots and stalk rots (such as anthracnose leaf bligh, anthrac- nose stalk rot, diplodia ear rot, Fusarium verticilioides, Gibberella zeae and top dieback, see e.g. US 2006/225152); apples with resistance against apple scab { Venturia inaequalis, see e.g. WO 1999064600); plants such as rice, wheat, barley, rye, corn, oats, potato, melon, soybean and sorghum with resistance against fusarium diseases, such as Fusarium graminearum, Fusarium sporotrichioides, Fusarium lateritium, Fusarium pseudograminearum Fusarium sam- bucinum, Fusarium culmorum, Fusarium poae, Fusarium acuminatum, Fusarium equiseti (see e.g. US 6646184, EP 1477557); plants, such as corn, soybean, cereals (in particular wheat, rye, barley, oats, rye, rice), tobacco, sorghum, sugarcane and potatoes with broad fungal resistance (see e.g. US 5689046, US 6706952, EP 1018553 and US 6020129).

Transgenic plants with resistance against bacterial pathogens and which are covered by the present invention, are, for examples, rice with resistance against Xylella fastidiosa (see e.g. US 6232528); plants, such as rice, cotton, soybean, potato, sorghum, corn, wheat, balrey, sugarcane, tomato and pepper, with resistance against bacterial blight (see e.g. WO 2006/42145, US 5952485, US 5977434, WO 1999/09151 , WO 1996/22375); tomato with resistance against Pseudomonas syringae (see e.g. Can. J. Plant Path., 1983, 5: 251 -255).

Transgenic plants with resistance against viral pathogens, are, for examples, stone fruits, such as plum, almond, apricot, cherry, peach, nectarine, with resistance against plum pox virus (PPV, see e.g. US PP15,154Ps, EP 0626449); potatoes with resistance against potato virus Y (see e.g. US 5968828); plants such as potato, tomato, cucumber and leguminosaes which are re- sistant against tomato spotted wilt virus (TSWV, see e.g. EP 0626449, US 5973135); corn with resistance against maize streak virus (see e.g. US 6040496); papaya with resistance against papaya ring spot virus (PRSV, see e.g. US 5877403, US 6046384); cucurbitaceae, such as cucumber, melon, watermelon and pumpkin, and solanaceae, such as potato, tobacco, tomato, eggplant, paprika and pepper, with resistance against cucumber mosaic virus (CMV, see e.g. US 6849780); cucurbitaceae, such as cucumber, melon, watermelon and pumkin, with resistance against watermelon mosaic virus and zucchini yellow mosaic virus (see e.g. US 6015942); potatoes with resistance against potato leafroll virus (PLRV, see e.g. US 5576202); potatoes with a broad resistance to viruses, such as potato virus X (PVX), potato virus Y (PVY), potato leafroll virus (PLRV) (see e.g. EP 0707069).

Further examples of deregulated orcommercially available transgenic plants with modified genetic material capable of expression of antipathogenic substances are the following plants: Carica papaya (papaya), Event: 55-1/63-1 ; Cornell University, Carica papaya (Papaya); Event: (X17-2); University of Florida, Cucurbita pepo (Squash); Event: (CZW-3); Asgrow (USA); Seminis Vegetable Inc. (Canada), Cucurbita pepo (Squash); Event: (ZW20); Upjohn (USA); Seminis Vegetable Inc. (Canada), Prunus domestica (Plum); Event: (C5); United States Department of Agriculture - Agricultural Research Service, Solanum tuberosum L. (Potato); Event: (RBMT15- 101 , SEMT15-02, SEMT15-15); Monsanto Company and Solanum tuberosum L. (Potato); Event: (RBMT21 -129, RBMT21 -350, RBMT22-082); Monsanto Company.

Transgenic plants with resistance against nematodes and which may be used in the methods of the present invention are, for examples, soybean plants with resistance to soybean cyst nematodes.

Methods have been proposed for the genetic transformation of plants in order to confer in- creased resistance to plant parasitic nematodes. U.S. Patent Nos. 5,589,622 and 5,824,876 are directed to the identification of plant genes expressed specifically in or adjacent to the feeding site of the plant after attachment by the nematode.

Also known in the art are transgenic plants with reduced feeding structures for parasitic nematodes, e.g. plants resistant to herbicides except of those parts or those cells that are nematode feeding sites and treating such plant with a herbicide to prevent, reduce or limit nematode feeding by damaging or destroying feeding sites (e.g. US 5866777).

Use of RNAi to target essential nematode genes has been proposed, for example, in PCT Publication WO 2001/96584, WO 2001/17654, US 2004/0098761 , US 2005/0091713, US

2005/0188438, US 2006/0037101 , US 2006/0080749, US 2007/0199100, and US

2007/0250947.

Transgenic nematode resistant plants have been disclosed, for example in the PCT publications WO 2008/095886 and WO 2008/095889.

Plants wich are resistant to antibiotics, such as kanamycin, neomycin and ampicillin. The natu- rally occurring bacterial nptll gene expresses the enzyme that blocks the effects of the antibiotics kanamycin and neomycin. The ampicillin resistance gene ampR (also known as blaTEMI ) is derived from the bacterium Salmonella paratyphi and is used as a marker gene in the transformation of micro-organisms and plants. It is responsible for the synthesis of the enzyme beta- lactamase, which neutralises antibiotics in the penicillin group, including ampicillin. Transgenic plants with resistance against antibiotics, are, for examples potato, tomato, flax, canola, oilseed rape and corn (see e.g. Plant Cell Reports, 20, 2001 , 610-615. Trends in Plant Science, 1 1 , 2006, 317-319. Plant Molecular Biology, 37, 1998, 287-296. Mol Gen Genet., 257, 1998, 606- 13.). Plant Cell Reports, 6, 1987, 333-336. Federal Register (USA), Vol.60, No.1 13, 1995, page 31 139. Federal Register (USA), Vol.67, No.226, 2002, page 70392. Federal Register (USA), Vol.63, No.88, 1998, page 25194. Federal Register (USA), Vol.60, No.141 , 1995, page 37870. Canadian Food Inspection Agency, FD/OFB-095-264-A, October 1999, FD/OFB-099-127-A, October 1999. Preferably, the plant is selected from soybean, maize (corn), rice, cotton, oilseed rape, potato, sugarcane, alfalfa, tomatoes and cereals, such as wheat, barley, rye and oat, most preferably from soybean, maize (corn), rice, cotton, oilseed rape, tomato, potato, vine, apple, pear, citron, orange and cereals such as wheat, barley, rye and oat.

Plants which are tolerant to stress conditions (see e.g. WO 2000/04173, WO 2007/131699, CA 2521729 and US 2008/0229448) are plants, which show increased tolerance to abiotic stress conditions such as drought, high salinity, high light intensities, high UV irradiation, chemical pollution (such as high heavy metal concentration), low or high temperatures, limitied supply of nutrients (i.e. nitrogen, phosphorous) and population stress. Preferably, transgenic plants with resistance to stress conditions, are selected from rice, corn, soybean, sugarcane, alfalfa, wheat, tomato, potato, barley, rapeseed, beans, oats, sorghum and cotton with tolerance to drought (see e.g. WO 2005/048693, WO 2008/002480 and WO 2007/030001 ); corn, soybean, wheat, cotton, rice, rapeseed and alfalfa with tolerance to low temperatures (see e.g. US 4731499 and WO 2007/1 12122); rice, cotton, potato, soybean, wheat, barley, rye, sorghum, alfalfa, grape, tomato, sunflower and tobacco with tolerance to high salinity (see e.g. US 7256326, US

7034139, WO 2001/030990). The methods of producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the publications mentioned above. Preferably, the plant is selected from soybean, maize (corn), rice, cotton, sugarcane, alfalfa, sugar beet, potato, oilseed rape, tomatoes and cereals such as wheat, barley, rye and oat, most preferably from soybean, maize (corn), rice, cotton, oilseed rape, tomato, potato, sugarcane, vine, apple, pear, citron, orange and cereals such as wheat, barley, rye and oat.

Altered maturation properties, are for example delayed ripening, delayed softening and early maturity. Preferably, transgenic plants with modified maturation properties, are, selected from tomato, melon, raspberry, strawberry, muskmelon, pepper and papaya with delayed ripening (see e.g. US 5767376, US 7084321 , US 6107548, US 5981831 , WO 1995035387, US

5952546, US 5512466, WO 1997001952, WO 1992/008798, Plant Cell. 1989, 53-63. Plant Molecular Biology, 50, 2002). The methods of producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the publications mentioned above. Preferably, the plant is selected from fruits, such as tomato, vine, melon, papaya, banana, pepper, raspberry and strawberry; stone fruits, such as cherry, apricot and peach; pome fruits, such as apple and pear; and citrus fruits, such as citron, lime, orange, pomelo, grapefruit, and mandarin_T more preferably from tomato, vine, apple, banana, orange and strawberry, most preferably tomatoes.

Content modification is synthesis of modified chemical compounds (if compared to the corresponding control plant) or synthesis of enhanced amounts of chemical (if compounds compared to the corresponding control plant) and corresponds to an increased or reduced amount of vitamins, amino acids, proteins and starch, different oils and a reduced amount of nicotine.

Commercial examples are the soybean varieties "Vistive II" and "Visitive III" with low- linolenic/medium oleic content; the corn variety "Mavera high-value corn" with increased lysine content; and the soybean variety "Mavera high value soybean" with yielding 5% more protein compared to conventional varieties when processed into soybean meal. Further transgenic plants with altered content are, for example, potato and corn with modified amylopectin content (see e.g. US 6784338, US 20070261 136); canola, corn, cotton, grape, catalpa, cattail, rice, soybean, wheat, sunflower, balsam pear and vernonia with a modified oil content (see e.g. US 7294759, US7157621 , US 5850026, US 6441278, US 6380462, US 6365802, US 6974898, WO 2001/079499, US 2006/0075515 and US 7294759); sunflower with increased fatty acid content (see e.g. US 6084164); soybeans with modified allergens content (so called "hypoaller- genic soybean, see e.g. US 6864362); tobacco with reduced nicotine content (see e.g. US 20060185684, WO 2005000352 and WO 2007064636); canola and soybean with increased lysine content (see e.g. Bio/Technology 13, 1995, 577 - 582); corn and soybean with altered composition of methionine, leucine, isoleucine and valine (see e.g. US 6946589, US 6905877); soybean with enhanced sulfur amino acid content (see e.g. EP 0929685, WO 1997041239); tomato with increased free amino acid contents, such as asparagine, aspartic acid, serine, threonine, alanine, histidine and glutamic acid (see e.g. US 672741 1 ); corn with enhanced amino acid content (see e.g. WO 050771 17); potato, corn and rice with modified starch content (see e.g. WO 1997044471 and US 7317146); tomato,corn, grape, alfalfa, apple, beans and peas with modified flavonoid content (see e.g. WO 2000/04175); corn, rice, sorghum, cotton, soybeans with altered content of phenolic compounds (see e.g. US 20080235829). The methods of producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the publications mentioned above. Preferably, the plant is selected from soybean, maize (corn), rice, cotton, sugarcane, potato, tomato, oilseed rape, flax and cereals such as wheat, barley, rye and oat, most preferably soybean, maize (corn), rice, oilseed rape, potato, tomato, cotton, vine, apple, pear, citron, orange and cereals such as wheat, barley, rye and oat.

Enhanced nutrient utilization is e.g. assimilation or metabolism of nitrogen or phosphorous. Preferably, transgenic plants with enhanced nitrogen assimilatory and utilization capacities are selected from for example, canola, corn, wheat, sunflower, rice, tobacco, soybean, cotton, alfalfa, tomato, wheat, potato, sugar beet, sugar cane and rapeseed (see e.g. WO 1995/00991 1 , WO 1997/030163, US 6084153, US 5955651 and US 6864405). Plants with improved phosphorous uptake are, for example, tomato and potato (see e.g. US 7417181 ). The methods of producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the publications mentioned above. Preferably, the plant is selected from soybean, maize (corn), rice, cotton, sugarcane, alfalfa, potato, oilseed rape and cereals such as wheat, barley, rye and oat, most preferably from soybean, maize (corn), rice, cotton, oilseed rape, tomato, potato, vine, apple, pear, citron, orange and cereals such as wheat, barley.

Transgenic plants with male steriliy are preferably selected from canola, corn, tomato, rice, Indian mustard, wheat, soybean and sunflower (see e.g. US 6720481 , US 6281348, US 5659124, US 6399856, US 7345222, US 7230168, US 6072102, EP1 135982, WO 2001/092544 and WO 1996/040949). The methods of producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the publications mentioned above. Preferably, the plant is selected from soybean, maize (corn), rice, cotton, oilseed rape, tomato, potato, vine, apple, pear, citron, orange and cereals such as wheat, barley. Further examples of deregulated or commercially available transgenic plants with modified genetic material being male sterile are

Brassica napus (Argentine Canola:(Event: MS1 , RF1 =>PGS1 ; Bayer CropScience (formerly Plant Genetic Systems); Brassica napus (Event: MS1 , RF2 =>PGS2 ; Bayer CropScience (for- merly Plant Genetic Systems); Brassica napus (Event: MS8xRF3 ; Bayer CropScience (Aventis CropScience(AgrEvo)); Brassica napus (Event: PHY14, PHY35 ; Bayer CropScience (formerly Plant Genetic Systems); Brassica napus (Event: PHY36 ; Bayer CropScience (formerly Plant Genetic Systems); Cichorium intybus (Chicory:(Event: RM3-3, RM3-4, RM3-6 ; Bejo Zaden BV; Zea mays L. (Maize:(Event: 676, 678, 680 ; Pioneer Hi-Bred International Inc.; Zea mays L. (Event: MS3 ; Bayer CropScience (Aventis CropScience(AgrEvo)) and Zea mays L. (Event: MS6 ; Bayer CropScience (Aventis CropScience(AgrEvo)).

Plants, which produce higher quality fiber are e.g. transgenic cotton plants. The such improved quality of the fiber is related to improved micronaire of the fiber, increased strength, improved staple length, improved length unifomity and color of the fibers (see e.g. WO 1996/26639, US 7329802, US 6472588 and WO 2001/17333). The methods of producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the publications mentioned above. As set forth above, cultivated plants may comprise one or more traits, e.g. selected from the group consisting of herbicide tolerance, insect resistance, fungal resistance, viral resistance, bacterial resistance, stress tolerance, maturation alteration, content modification, modified nutrient uptake and male sterility (see e.g. WO 2005033319 and US 6376754). Examples of commercial available transgenic plants with two combined properties are the corn varieties "YieldGard Roundup Ready" and YieldGard Roundup Ready 2" (Monsanto) with glyphosate tolerance and resistance to corn borer; the corn variety "Agrisure CB/LL" (Syntenta) with glufosinate tolerance and corn borer resistance; the corn variety "Yield Gard VT Root- worm/RR2" with glyphosate tolerance and corn rootworm resistance; the corn variety "Yield Gard VT Triple" with glyphosate tolerance and resistance against corn rootworm and corn borer; the corn variety "Herculex I" with glufosinate tolerance and lepidopteran resistance (Cry1 F), i.e. against western bean cutworm, corn borer, black cutworm and fall armyworm; the corn variety "YieldGard Corn Rootworm/Roundup Ready 2" (Monsanto) with glyphosate tolerance and corn rootworm resistance; the corn variety "Agrisure GT/RW" (Syngenta) with gluphosinate tolerance and lepidopteran resistance (Cry3A), i.e. against western corn rootworm, northern corn root- worm and Mexican corn rootworm; the corn variety "Herculex RW" (Dow, Pioneer) with glufosinate tolerance and lepidopteran resistance (Cry34/35Ab1 ), i.e. against western corn rootworm, northern corn rootworm and Mexican corn rootworm; the corn variety "Yield Gard VT Rootworm/RR2" with glyphosate tolerance and corn rootworm resistance; the soybean variety "Optimum GAT" (DuPont, Pioneer) with glyphosate tolerance and ALS herbicide tolerance; the corn variety "Mavera high-value corn" with glyphosate tolerance, resistance to corn rootworm and European corn borer and high lysine trait.

Examples of commercial available transgenic plants with three traits are the corn variety "Herculex I / Roundup Ready 2" with glyphosate tolerance, gluphosinate tolerance and lepidopteran resistance (Cry1 F), i.e. against western bean cutworm, corn borer, black cutworm and fall armyworm; the corn variety "YieldGard Plus / Roundup Ready 2" (Monsanto) with glyphosate tolerance, corn rootworm resistance and corn borer resistance; the corn variety "Agrisure GT/CB/LL" (Syngenta) with tolerance to glyphosate tolerance, tolerance to gluphosinate and corn borer resistance; the corn variety "Herculex Xtra" (Dow, Pioneer) with glufosinate tolerance and lepidopteran resistance (Cry1 F + Cry34/35Ab1 ), i.e. against western corn rootworm, northern corn rootworm, Mecxican corn rootworm, western bean cutworm, corn borer, black cutworm and fall armyworm; the corn varieties "Agrisure CB/LL/RW" (Syngenta) with glufosinate tolerance, corn borer resistance (Cry1 Ab) and lepidopteran resistance (Cry3A), i.e. against western corn rootworm, northern corn rootworm and Mexican corn rootworm; the corn variety "Agrisure 3000GT" (Syngenta) with glyphosate tolerance + corn borer resistance (CrylAb) and lepidopteran resistance (Cry3A), i.e. against western corn rootworm, northern corn rootworm and Mexican corn rootworm. The methods of producing such transgenic plants are generally known to the person skilled in the art.

An example of a commercial available transgenic plant with four traits is„Hercules Quad-Stack" with glyphosate tolerance, glufosinate tolerance, corn borer resistance and corn rootworm resistance.

Embodiments and preferred methods of the present invention

In one embodiment of the invention, the commercial transgenic plant is a soybean variety with glyphosate tolerance and lepidopteran resistance, preferably with one trait of glyphosate tolerance and two traits of lepidopteran resistance. Preferably, the glyphosate tolerance is through expression of the EPSPS encoding gene from A. tumefaciens strain CP4 (cp4epsps gene), more preferably it is based on the transgenic event MON89788 (see A1 -14, T1 -100). Also preferably, the lepidopteran resistance is a resistance to lepidopteran pests of soybean, preferably through expresssion of the CrylAC encoding gene from B. thuringiensis, preferably against vel- vetbean caterpillar (Anticarsia gemmatalis) and soybean looper (Pseudoplusia includens (synonym Chrysodeixis includens)), more preferably it is based on the transgenic event MON87701. More preferably, the glyphosate tolerance is based on the transgenic event MON89788 and the trait of lepidopteran resistance is achieved through expresssion of the CrylAC encoding gene from B. thuringiensis, preferably against velvetbean caterpillar (Anticarsia gemmatalis) and soybean looper (Pseudoplusia includens), more preferably based on the transgenic event

MON87701 .

Most preferably, the commercial transgenic plant is "Intacta RR2 PRO" soybean (Monsanto) which claims to offer tolerance to glyphosate herbicide and protection against major soybean pests (velvetbean caterpilar, soybean looper, soybean budborer, bean shoot borer, bollworm, corn stalk borer, Helicoverpa, e.g. Helicoverpa armigera), along with increased yield potential. It is understood that "Intacta RR2 Pro" is used as a synonym for "Intacta™ Roundup Ready™ 2 Pro" soybean variety. Therefore, in one embodiment, the invention relates to methods according to the invention wherein the cultivated plant is a soybean variety with glyphosate tolerance and lepidopteran resistance.

In a further embodiment, the invention relates to methods according to the invention, wherein the glyphosate tolerance is through the expression of the cp4epsps gene, or wherein the lepidopteran resistance is through expresssion of the CrylAC encoding gene from B. thuringiensis, or wherein both the glyphosate tolerance is through the expression of the cp4epsps gene and the lepidopteran resistance is through expresssion of the CrylAC encoding gene from B. thuringiensis.

In a further embodiment, the invention relates to methods according to the invention, wherein the glyphosate tolerance is based on the transgenic event MON89788, or wherein the lepidopteran resistance is based on the transgenic event MON87701 , or wherein both the glyphosate tolerance is based on the transgenic event MON89788 and the lepidopteran resistance is based on the transgenic event MON87701 .

In a further embodiment, the invention relates to methods according to the invention, wherein the lepidopteran resistance is against a species selected from the group of velvetbean caterpillar (Anticarsia gemmatalis) and soybean looper (Pseudoplusia includens, Chrysodeixis in- cludens ).

In a further embodiment, the invention relates to methods according to the invention, wherein the cultivated plant is "Intacta RR2 PRO" soybean (Monsanto), which claims to offer tolerance to glyphosate herbicide and protection against major soybean pests (velvetbean caterpilar, soybean looper, soybean budborer, bean shoot borer, bollworm, corn stalk borer, Helicoverpa, e.g. Helicoverpa armigera), along with increased yield potential.

In a further embodiment, the invention relates to such methods according to the invention, wherein the pest is selected from the group of stinkbug species (preferably Euschistus heros), Spodoptera frugiperda and Helicoverpa.

In another embodiment, the commercial transgenic plant is a soybean variety selected from "Roundup Ready 2 Yield", "Intacta RR2 Pro" and "Vistive Gold" (all Monsanto), or "Stearidonic Acid (SDA) Omega-3" (higher content of SDA in soybean, Monsanto). In another embodiment, the trait is Bacillus thuringiensis Cry1A.105 and cry2Ab2 and Vector PV-GMIR13196, for Mon87751 soybean (Monsanto).

In a further embodiment, the commercial transgenic plant is a soybean variety with herbicide tolerance and lepidopteran resistance, wherein the control of Lepidopteran pest is based on Bt CrylAc and Cryl F toxins. Preferably, the insect-resistant and herbicide-tolerant soybean is DAS81419 (see Table A1 , entry A1 -334).

Preferably, the plant has one trait of glyphosate tolerance and two traits of lepidopteran resistance. Preferably, the glyphosate tolerance is through expression of the EPSPS encoding gene from A. tumefaciens strain CP4, more preferably it is based on the transgenic event

MON89788. Also preferably, the lepidopteran resistance is a resistance to lepidopteran pests of soybean, preferably through expresssion of the CrylAC encoding gene from B. thuringiensis, preferably against velvetbean caterpillar (Anticarsia gemmatalis) and soybean looper (Pseudoplusia includens), more preferably it is based on the transgenic event MON87701. More preferably, the glyphosate tolerance is based on the transgenic event MON89788 and the trait of lepidopteran resistance is achieved through expresssion of the CrylAC encoding gene from B. thuringiensis, preferably against velvetbean caterpillar (Anticarsia gemmatalis) and soybean looper (Pseudoplusia includens), more preferably based on the transgenic event

MON87701 . Pseudoplusia includens is a synonym for Chrysodeixis includens.

Most preferably, the commercial transgenic plant is "Intacta RR2 PRO" soybean (Monsanto) which claims to offer tolerance to glyphosate herbicide and protection against major soybean pests (velvetbean caterpilar, soybean looper, soybean budborer, bean shoot borer, bollworm, corn stalk borer, Helicoverpa, e.g. Helicoverpa armigera), along with increased yield potential.

In a further embodiment, the commercial transgenic plant is a corn variety which has above- ground insect protection from "Genuity VT Triple PRO" or "Herculex Xtra" or both of them, and herbicide tolerance from "Roundup Ready 2" and Liberty Link, preferably corn varieties selected from "Genuity SmartStax", "Genuity VT Triple PRO" and "Genuity VT Double PRO" (all Monsan- to), optionally as RIB (refuge-in-bag) solution. In a further embodiment, the commercial transgenic corn plant variety has a drought tolerance trait, preferably "Genuity DroughtGard". In another embodiment, the trait is double-stranded ribonucleic acid (dsRNA), Bacillus thuringiensis Cry3Bb1 protein and vector PV-ZMIR10871 for MON8741 1 corn. In a further embodiment, the commercial transgenic plant is a cotton variety selected from "Boll- gard II" (insect protection), "Roundup Ready Flex" (herbicide tolerance) and "Bollgard II with Roundup Ready Flex" (both), all Monsanto.

In one embodiment of the invention the cultivated plant is selected from the group of plants as mentioned in the paragraphs and tables of this disclosure. The plants listed in following tables have a property or a transgenic event as described in one row of the table.

Preferably, the cultivated plants are plants, which comprise at least one trait selected from herbicide tolerance, insect resistance for example by expression of one or more bacterial toxins, fungal resistance or viral resistance or bacterial resistance by expression of one or more anti- pathogenic substances, stress tolerance, nutrient uptake, nutrient use efficiencyor content modification of chemicals present in the cultivated plant compared to the corresponding control plant.

More preferably, the cultivated plants are plants, which comprise at least one trait selected from herbicide tolerance, insect resistance by expression of one or more bacterial toxins, fungal resistance or viral resistance or bacterial resistance by expression of one or more antipathogenic substances, stress tolerance or content modification of one or more chemicals present in the cultivated plant compared to the corresponding control plant. Most preferably, the cultivated plants are plants, which are tolerant to the action of herbicides. Most preferably, the cultivated plants are plants, which express bacterial toxins, which provides resistance against animal pests (such as insects or arachnids or nematodes), wherein the bacterial toxin is preferably a toxin from Bacillus thuriginensis. Most preferably, the cultivated plants are both, namely plants, which are tolerant to the action of herbicides plants, which express bacterial toxins, which provides resistance against animal pests (such as insects or arachnids or nematodes), and wherein the bacterial toxin is preferably a toxin from Bacillus thuriginensis.

Herein, the plant is preferably selected from cotton, rice, maize (corn), wheat, barley, rye, oat, soybean, potato, vine, apple, pear, citron and orange.

In one preferred embodiment, the plant is soybean.

In one preferred embodiment, the plant is cotton.

In one preferred embodiment, the plant is maize (or corn).

For illustration purposes, the following table A provides examples of cultivated plants, especially crops (with their event name and their event code and further identifying details ), in the sense of the present invention.

Thus, according to the invention, afidopyropen is used alone or in mixtures in methods for the application on the crops listed in table A, wherein each method corresponds to the application on a respective crop of each line of table A.

In addition to table A below, also the following crops (listed herein below with their event name and event code) may illustrate examples of cultativated plants in the meaning of the present invention:

Alfalfa: KK179 x J101 - MON-00179-5 x MON-00101 -8

Apple: GD743 - OKA-NB001 -8

Apple: GD784 - OKA-NB002-9

· Cotton: GHB614 x T304-40 x GHB1 19 x COT102 - BCS-GH002-5 x BCS-GH004-7 x BCS-GH005-8 x SYN-IR102-7

Cotton: MON88701 - MON 88701 -3

Cotton: MON88701 x MON88913 - MON 88701 -3 x MON-88913-8

Cotton: MON88701 x MON88913 x MON15985 MON 88701 -3 x MON-88913-8 x MON- 15985-7

Eucalyptus: GM Eucalyptus - H421

Maize: 3272 x Bt1 1 x MIR604 x TC1507 x 5307 x GA21 - SYN-E3272-5 x SYN-BT01 1 -1 x SYN-IR604-5 x DAS-01507-1 x SYN-05307-1 x MON-00021 -9

Maize: MIR162 x NK603SYN-IR162-4 x MON-00603-6

· Maize: MON81 O x MIR162 - MON-00810-6 X SYN-IR162-4

Maize: TC1507 x MIR162 x NK603 - DAS-01507-1 x SYN-IR162-4 x MON-00603-6 Maize: TC1507 x MON810 x MIR162 - DAS-01507-1 x MON-00810-6 x SYN-IR162-4 Potato: AM04-1020 - BPS-A1020-5

Soybean: MON87712 - MON-87712-4 Table A

MHR. Methods for herbicide resistant plants

In one embodiment of the present invention, the cultivated plants are plants, which are tolerant to the action of herbicides. In another embodiment, the plants may have the tolerance to the action of herbicides in addition to an insecticidal trait. (For methods for plants having combined resistance see further below).

If such plants are used in the methods according to the present invention, afidopyropen and its mixtures may additionally comprise a herbicide III, to which the plant is tolerant.

For example, if the cultivated plant is a cultivated plant tolerant to glyphosate, afidopyropen and its mixtures may additionally comprise glyphosate.

For example, if the cultivated plant is a cultivated plant tolerant to glufonsinate, afidopyropen and its mixtures may additionally comprise glufonisate.

For example, if the cultivated plant is a cultivated plant tolerant to a imidazolione herbicide, afidopyropen and its mixtures may additionally comprise at least one imidazolione-herbicide. Herein, the imidazolionone-herbicide is selected from imazamox, imazethapyr, imazapic, ima- zapyr, imazamethabenz or imazaquin.

For example, if the cultivated plant is a cultivated plant tolerant to dicamba, afidopyropen and its mixtures may additionally comprise dicamba.

For example, if the cultivated plant is a cultivated plant tolerant to sethoxidim, afidopyropen and its mixtures may additionally comprise sethoxidim.

For example, if the cultivated plant is a cultivated plant tolerant to cycloxidim, afidopyropen and its mixtures may additionally comprise cycloxidim. Thus, the present invention also relates to ternary mixtures, comprising afidopyropen, an insecticide II and a herbicide III.

In another embodiment of the present invention, the present invention also relates to ternary mixtures comprising two insecticides, wherein one insecticide is afidopyropen, and a fungicide. In another particular embodiment, the present invention also relates to ternary mixtures com- prising two fungicides and one insecticide, wherein the one insecticide is afidopyropen.

In another particular embodiment, the present invention also relates to ternary mixtures comprising one insectide, wherein one insecticide is afidopyropen, a fungicide and a herbicide.

In utmost preference, the cultivated plants are plants, which are given in table A. Sources: Ag- Bios database and GMO-compass database (AG BIOS, P.O. Box 475, 106 St. John St. Mer- rickville, Ontario KOG1 NO, Canada, access: http://cera-gmc.org/, also see BioTechniques, Volume 35, No. 3, Sept. 2008, p. 213, and http://www.gmo-compass.org/eng/gmo/db/).

Thus, in one preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials, or at their locus of growth with compound of formula I and its mixtures, wherein the plant is a plant, which is rendered tolerant to herbicides, more preferably to herbicides such as glutamine synthetase inhibitors, 5-enol-pyrovyl-shikimate- 3-phosphate-synthase inhibitors, acetolactate synthase (ALS) inhibitors, protoporphyrinogen oxidase (PPO) inhibitors, auxine type herbicides, most preferably to herbicides such as glypho- sate, glufosinate, imazapyr, imazapic, imazamox, imazethapyr, imazaquin, imazamethabenz methyl, dicamba and 2,4-D.

In a more preferred embodiment, the present invention relates to a method of controlling harmful pests, especially insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials or their locus of growth with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, preferably an insecticide or/and a fungicide, wherein the plant corresponds to a row of table A.1 .

Furthermore, the invention relates to a method for increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials, or at their locus of growth with the compound of formula I, wherein the plant corresponds to a row of table A1.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with afidopyropen or mixtures of afidopyropene comprising another pesticidal active ingredient, preferably another insecticide and/or fungicide, wherein the plant corresponds to row of table A.1 .

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or their locus of growth with afidopyropen, wherein the plant corresponds to a row of table A.1.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or their locus of growth with a mixture of afidopyropen with abamectin, wherein the plant corresponds to a row of table A.1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or their locus of growth with a mixture of afidopyropen with dinotefuran, wherein the plant corresponds to a row of table A.1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or their locus of growth with a mixture of afidopyropen with ethiprole, wherein the plant corresponds to a row of table A.1 . In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or their locus of growth with a mixture of afidopyropen with fipronil, wherein the plant corresponds to a row of table A.1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or their locus of growth with a mixture of afidopyropen with chlorfenapyr, wherein the plant corresponds to a row of table A.1.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or their locus of growth with a mixture of afidopyropen with a-cypermethrin, wherein the plant corresponds to a row of table A.1.

Table A1

No description transgenic plant literature / commercial

event plants

A1 -1 Glyphosate ASR368 Agrostis stolonifera available, Scotts Seeds

tolerance (creeping bent- grass)

A1 -2 Glyphosate A5-15 Beta vulgaris (sugavailable, Danisco

tolerance ar beet) Seeds / DLF Trifolium

A1 -3 Glyphosate GTSB77 Beta vulgaris (sugavailable, Novartis

tolerance ar beet) Seeds; Monsanto

Company

A1 -4 Glyphosate H7-1 Beta vulgaris (sugavailable, Monsanto

tolerance ar beet) Company

A1 -5 Glyphosate T120-7 Beta vulgaris (sugavailable, Bayer

tolerance ar beet) CropScience (Aventis

CropScience(AgrEvo))

A1 -6 Glyphosate GT200 Brassica napus available, Monsanto

tolerance (Argentine canola) Company

A1 -7 Glyphosate GT73, Brassica napus available, Monsanto

tolerance RT73 (Argentine canola) Company

A1 -8 Glyphosate HCN10 Brassica napus available, Aventis

tolerance (Argentine canola) CropScience

A1 -9 Glyphosate HCN92 Brassica napus available, Bayer

tolerance (Argentine canola) CropScience (Aventis

CropScience(AgrEvo))

A1 -10 Glyphosate T45 Brassica napus available, Bayer

tolerance (HCN28) (Argentine canola) CropScience (Aventis

CropScience(AgrEvo)) A1 -1 1 Glyphosate ZSR500/5 Brassica rapa (Poavailable, Monsanto tolerance 02 lish canola) Company

A1 -12 Glyphosate GTS 40-3- Glycine max L. available, Monsanto tolerance 2 (soybean) Company

A1 -13 Glyphosate MON40-3- Glycine max L. available, Monsanto tolerance 2 (soybean) Company

A1 -14 Glyphosate MON8978 Glycine max L. available, Monsanto tolerance 8 (soybean) Company

A1 -15 Glyphosate GHB614 Gossypium hirsu- available, Bayer Crop- tolerance tum L (cotton) Science USA LP

A1 -16 Glyphosate MON1445 Gossypium hirsu- available, Monsanto tolerance tum L (cotton) Company

A1 -17 Glyphosate MON1445 Gossypium hirsu- available, Monsanto tolerance /1698 tum L (cotton) Company

A1 -18 Glyphosate MON8891 Gossypium hirsu- available, Monsanto tolerance 3 tum L (cotton) Company

A1 -19 Glyphosate MON- Medicago sativa available, Monsanto tolerance 00101 -8, (alfalfa) and Forage Genetics

MON- International

00163-7

(J101 ,

J 163)

A1 -20 Glyphosate MON7180 Triticum aestivum available, Monsanto tolerance 0 (wheat) Company

A1 -21 Glyphosate NK603 Zea mays L. (corn, available, Monsanto tolerance maize) Company

A1 -22 Glyphosate GA21 Zea mays L. (corn, available, Syngenta tolerance maize) Seeds, Inc. (formerly

A1 -23 Glyphosate MON832 Zea mays L. (corn, Monsanto Company tolerance maize)

A1 -24 Glufosinate GS40 / Brassica napus available, Bayer Crop- tolerance 90pHoe6 / (Argentine canola) Science

Ac

A1 -25 Glufosinate Liberator Brassica napus available, Bayer Crop- tolerance pHoe6/Ac (Argentine canola) Science

A1 -26 Glufosinate TOPAS Brassica napus available, Bayer Crop- tolerance 19/2 (Argentine canola) Science

A1 -27 Glufosinate T14, T25 Zea mays L. (corn, Bayer CropScience tolerance (ACS- maize) (Aventis

ZM002-1 CropScience(AgrEvo)) / ACS- A1 -28 Glufosinate PHY14, Brassica napus available, Aventis ammonium tolPHY35 (Argentine canola) CropScience (formerly erance Plant Genetic Systems)

A1 -29 Glufosinate PHY36 Brassica napus available, Aventis

ammonium tol(Argentine canola) CropScience (formerly erance Plant Genetic Systems)

A1 -30 Glufosinate HCR-1 Brassica rapa (Poavailable, Bayer

ammonium tollish canola) CropScience (Aventis erance CropScience(AgrEvo))

A1 -31 Glufosinate RM3-3, Cichorium intybus available, Bejo Zaden ammonium tolRM3-4, (Chicory) BV

erance RM3-6

A1 -32 Glufosinate A2704-12, Glycine max L. available, Bayer

ammonium tolA2704-21 , (soybean) CropScience (Aventis erance A5547-35 CropScience(AgrEvo))

A1 -33 Glufosinate A5547- Glycine max L. available, Bayer

ammonium tol127 (soybean) CropScience (Aventis erance CropScience(AgrEvo))

A1 -34 Glufosinate GU262 Glycine max L. available, Bayer

ammonium tol(soybean) CropScience (Aventis erance CropScience(AgrEvo))

A1 -35 Glufosinate W62, W98 Glycine max L. available, Bayer

ammonium tol(soybean) CropScience (Aventis erance CropScience(AgrEvo))

A1 -36 Glufosinate LLCotton2 Gossypium hirsu- available, Bayer

ammonium tol- 5 tum L (cotton) CropScience (Aventis

A1 -37 Glufosinate LL RICE Oryza sativa (rice) available, Bayer Crop- ammonium tol62 Science

erance

A1 -38 Glufosinate LLrice06 Oryza sativa (rice) available, Bayer Crop- ammonium tolLLrice 62 Science

erance

A1 -39 Glufosinate LLrice601 Oryza sativa (rice) available, Bayer Crop- ammonium tolScience

erance

A1 -40 Glufosinate 676, 678, Zea mays L. (corn, available, Pioneer Hi- ammonium tol- 680 maize) Bred International Inc.

A1 -41 Glufosinate B16 Zea mays L. (corn, available, Dekalb Geammonium tol- (DLL25) maize) netics Corporation

A1 -42 Imidazolinone NS738, Brassica napus available, Pioneer Hi- tolerance NS1471 , (Argentine canola) Bred International Inc. A1 -43 Imidazolinone X81359 He/fan thus annuus available, BASF tolerance (sunflower)

A1 -44 Imidazolinone RH44 Lens culinaris (lenavailable, BASF

tolerance til)

A1 -45 Imidazolinone CFX51 Oryza sativa (rice) available, BASF

tolerance

A1 -46 Imidazolinone IMINTA-1 , Oryza sativa (rice) available, BASF

tolerance IMINTA-4

A1 -47 Imidazolinone PWC16 Oryza sativa (rice) available, BASF

tolerance

A1 -48 Imidazolinone AP205CL Triticum aestivum available, BASF Inc.

tolerance (wheat)

A1 -49 Imidazolinone AP602CL Triticum aestivum available, BASF Inc.

tolerance (wheat)

A1 -50 Imidazolinone BW255-2, Triticum aestivum available, BASF Inc.

tolerance BW238-3 (wheat)

A1 -51 Imidazolinone BW7 Triticum aestivum available, BASF Inc.

tolerance (wheat)

A1 -52 Imidazolinone SWP9650 Triticum aestivum available, Cyanamid tolerance 01 (wheat) Crop Protection

A1 -53 Imidazolinone Teal 1 1A Triticum aestivum available, BASF Inc.

tolerance (wheat)

A1 -54 Imidazolinone 3751 IR Zea mays L. (corn, available, Pioneer Hi- tolerance maize) Bred International Inc.

A1 -55 Imidazolinone EXP1910I Zea mays L. (corn, available, Syngenta tolerance T maize) Seeds, Inc. (formerly

A1 -56 Imidazolinone IT Zea mays L. (corn, available, Pioneer Hi- tolerance maize) Bred International Inc.

A1 -57 sulfonyl urea 19-51 A Gossypium hirsu- available, DuPont tolerance tum L. (cotton) Canada Agricultural

A1 -58 sulfonyl urea CDC- University of Sasavailable, Linum usita- tolerance FL001 -2 katchewan, Crop tissimum L. (flax, lin¬

(FP967) Dev. Centre seed)

A1 -59 Bromoxynil and OXY-235 Brassica napus available, Aventis loxynil toleran(Argentine canola) CropScience (formerly ce Rhone Poulenc Inc.)

A1 -60 Bromoxynil and BXN Gossypium hirsu- available, Calgene Inc.

loxynil toleran- tum L (cotton) A1 -61 Bromoxynil and C/F/93/08- Nicotiana tabacum available, Societe Naloxynil toleran02 L. (tobacco) tional d'Exploitation ce des Tabacs et Allu- mettes

A1 -62 Cyclohexanone DK404SR Zea mays L. (corn, available, BASF Inc.

tolerance maize)

In another embodiment of the present invention, the set of methods decribed above for table A.1 concerning afidopyropen and its individualized mixtures are also to be applied, when the plant corresponds to a row of table 1.

Table 1

No detailed description [Event] plant Literature / commercial plants

T1 -1 imidazolinone tolerance canola B^*

T1 -2 imidazolinone tolerance maize A^*, B^*

T1 -3 imidazolinone tolerance rice A^*, C^*

T1 -4 imidazolinone tolerance millet A^*

T1 -5 imidazolinone tolerance barley A^*

T1 -6 imidazolinone tolerance wheat A^*

T1 -7 imidazolinone tolerance sorghum A^*

T1 -8 imidazolinone tolerance oats A^*

T1 -9 imidazolinone tolerance rye A^*

T1 -10 imidazolinone tolerance sugar beet WO 1998/02526 / WO 1998/02527

T1 -1 1 imidazolinone tolerance lentils US2004/0187178

T1 -12 imidazolinone tolerance sunflowers B^*

T1 -13 imidazolinone tolerance wheat D^*

T1 -14 glyphosate tolerance alfalfa E^*; "Roundup Ready Alfalfa"

T1 -15 glyphosate tolerance apple E^*

T1 -16 glyphosate tolerance barley E^*

T1 -17 glyphosate tolerance canola E^*; V^*

T1 -18 glyphosate tolerance maize E^*; W^*

T1 -19 glyphosate tolerance cotton E^*; X^*

T1 -20 glyphosate tolerance flax E^*

T1 -21 glyphosate tolerance grape E^*

T1 -22 glyphosate tolerance lentil E^*

T1 -23 glyphosate tolerance oil seed E^*

rape

T1 -24 glyphosate tolerance pea E^*

T1 -25 glyphosate tolerance potato E^*

T1 -26 glyphosate tolerance rice "Roundup Ready Rice" (Monsanto)

T1 -27 glyphosate tolerance soybean E^*; Y^*

T1 -28 glyphosate tolerance sugar beet E^* No detailed description [Event] plant Literature / commercial plants

T1-29 glyphosate tolerance sunflower E^*

T1-30 glyphosate tolerance tobacco E^*

T1-31 glyphosate tolerance tomato E^*

T1-32 glyphosate tolerance turf grass E^*

T1-33 glyphosate tolerance wheat E^*

T1-34 gluphosinate tolerance canola F^*; U^*

T1-35 gluphosinate tolerance maize F^*; Z^*

T1-36 gluphosinate tolerance cotton F^*; "FiberMax Liberty Link" (Bayer),

T1-37 gluphosinate tolerance potato F^*

T1-38 gluphosinate tolerance rice F^*, G^*; "Liberty Link Rice" (Bayer),

T1-39 gluphosinate tolerance sugar beet F^*

T1-40 gluphosinate tolerance soybean US6376754

T1-41 gluphosinate tolerance tobacco F^*

T1-42 gluphosinate tolerance tomato F^*

T1-43 dicamba tolerance bean US7105724

T1-44 dicamba tolerance maize US7105724, WO2008051633

T1-45 dicamba tolerance cotton US7105724, US5670454

T1-46 dicamba tolerance pea US7105724

T1-47 dicamba tolerance potato US7105724

T1-48 dicamba tolerance sorghum US7105724

T1-49 dicamba tolerance soybean US7105724, US5670454

T1-50 dicamba tolerance sunflower US7105724

T1-51 dicamba tolerance tobacco US7105724

T1-52 dicamba tolerance tomato US7105724, US5670454

T1-53 bromoxynil tolerance canola "Navigator", "Compass" (Rhone- Poulenc)

T1-54 bromoxynil tolerance cotton "BXN" (calgene)

T1-55 2,4-D tolerance apple H^*

T1-56 2,4-D tolerance maize H^*

T1-57 2,4-D tolerance cotton US5670454

T1-58 2,4-D tolerance cucumber H^*

T1-59 2,4-D tolerance pepper H^*

T1-60 2,4-D tolerance potato H^*

T1-61 2,4-D tolerance sorghum H^*

T1-62 2,4-D tolerance soybean H^*

T1-63 2,4-D tolerance sunflower H^*

T1-64 2,4-D tolerance tobacco H^*

T1-65 2,4-D tolerance tomato H^*

T1-66 2,4-D tolerance wheat H^*

T1-67 HPPD inhibitor tolerance barley I^*

(K^*) No detailed description [Event] plant Literature / commercial plants

T1 -68 HPPD inhibitor tolerance maizef I *

(K^*)

T1 -69 HPPD inhibitor tolerance cotton I *

(K^*)

T1 -70 HPPD inhibitor tolerance potato I *

(K^*)

T1 -71 HPPD inhibitor tolerance rapeseed I *

(K^*)

T1 -72 HPPD inhibitor tolerance rice I *

(K^*)

T1 -73 HPPD inhibitor tolerance soybean I*

(K^*)

T1 -74 HPPD inhibitor tolerance sutarbeet I *

(K^*)

T1-75 HPPD inhibitor tolerance sugarcane I*

(K^*)

T1 -76 HPPD inhibitor tolerance tobacco I *

(K^*)

T1 -77 HPPD inhibitor tolerance wheat I *

(K^*)

T1 -78 Protox inhibitor tolerance cotton M^*

(L^*)

T1 -79 Protox inhibitor tolerance rape M^*

(L^*)

T1 -80 Protox inhibitor tolerance rice M^*

(L^*)

T1 -81 Protox inhibitor tolerance sorghum M^*

(L^*)

T1 -82 Protox inhibitor tolerance soybean M^*

(L^*)

T1 -83 Protox inhibitor tolerance sugarbeet M^*

(L^*)

T1 -84 Protox inhibitor tolerance sugar M^*

(L^*) cane

T1 -85 Protox inhibitor tolerance wheat M^*

(L^*)

T1 -86 imidazolinone tolerance soybean N^*

A* refers to US 4761373, US 5304732, US 5331 107, US 5718079, US 621 1438, US 621 1439 and US 6222100.

B^* refers to Tan et. al, Pest Manag. Sci 61 , 246-257 (2005). C^* refers to imidazolinone-herbicide resistant rice plants with specific mutation of the acetohy- droxyacid synthase gene: S653N ( see e.g. US 2003/0217381 ), S654K ( see e.g. US

2003/0217381 ), A122T (see e.g. WO 2004/106529) S653(At)N, S654(At)K, A122(At)T and other resistant rice plants as described in WO 2000/27182, WO 2005/20673 and WO 2001/85970 or US patents US 5545822, US 5736629, US 5773703, US 5773704, US- 5952553, US 6274796, wherein plants with mutation S653A and A122T are most preferred.

D^* refers to WO 2004/106529, WO 2004/16073, WO 2003/14357, WO 2003/13225 and WO 2003/14356.

E^* refers to US 5188642, US 4940835, US 5633435, US 5804425 and US 5627061 .

F^* refers to US 5646024 and US 5561236.

G^* refers to US 6333449, US 69331 1 1 and US 6468747.

H^* refers to US 6153401 , US 6100446, WO 2005/107437, US 5670454 and US 5608147. I^* refers to WO 2004/055191 , WO 199638567 and US 6791014.

K^* refers to HPPD inhibitor herbicides, such as isoxazoles (e.g. isoxaflutole), diketonitriles, trikeones (e.g. sulcotrione and mesotrione), pyrazolinates.

L^* refers to protoporphyrinogen oxidase (PPO) inhibiting herbicides.

M^* refers to US 2002/0073443, US 20080052798, Pest Management Science, 61 , 2005, 277- 285.

N^* refers to the herbicide tolerant soybean plants presented under the name of Cultivance on the XVI Congresso Brasileiro de Sementes, 31 st Augusta to 3^rd September 2009 at Estagao Embratel Convention Center - Curitiba/PR, Brazil

U^* "InVigor" (Bayer)

V^* "Roundup Ready Canola" (Monsanto)

W^*"Roundup Ready Corn", "Roundup Ready 2" (Monsanto),

"Agrisure GT", "Agrisure GT/CB/LL", "Agrisure GT/RW",„Agrisure 3000GT" (Syngenta),

"YieldGard VT Rootworm/RR2", "YieldGard VT Triple" (Monsanto)

X^* "Roundup Ready Cotton", "Roundup Ready Flex" (Monsanto)

Y^* "Roundup Ready Soybean" (Monsanto), "Optimum GAT" (DuPont, Pioneer)

Z^* "Liberty Link" (Bayer),

"Herculex I", "Herculex RW", "Herculex Xtra"(Dow, Pioneer),

"Agrisure GT/CB/LL", "Agrisure CB/LL/RW" (Syngenta),

In an especially preferred embodiment the methods, as described above for table A.1 and table 1 , concerning afidopyropen and its individualized mixtures are also to be applied, when the plant is selected from the especially preferred subset of herbicide tolerant plants given in table 2.

Table 2

No detailed description plant Literature / commercial plants

T2-1 imidazolinone tolerance canola B^*

T2-2 imidazolinone tolerance maize A^*, B^*

T2-3 imidazolinone tolerance rice C^*

T2-4 imidazolinone tolerance sunflowers B^* No detailed description plant Literature / commercial plants

T2-5 imidazolinone tolerance wheat D^*

T2-6 glyphosate tolerance alfalfa E^* "Roundup Ready Alfalfa"

T2-7 glyphosate tolerance canola E^* u^*

T2-8 glyphosate tolerance maize E^* V^*

T2-9 glyphosate tolerance cotton E^* W^*

glyphosate tolerance rice E^* "Roundup Ready Rice" (Monsan-

T2-10

to)

T2-1 1 glyphosate tolerance soybean E^*; X^*

T2-12 glyphosate tolerance sugar beet E^*

T2-13 glufosinate tolerance canola F^* "InVigor" (Bayer)

T2-14 glufosinate tolerance maize F^* Y_*

T2-15 glufosinate tolerance cotton F^* "FiberMax Liberty Link" (Bayer),

T2-16 glufosinate tolerance rice F^*, G^*; "Liberty Link Rice" (Bayer),

T2-17 glufosinate tolerance soybean I^*

T2-18 dicamba tolerance cotton US 7105724

T2-19 dicamba tolerance soybean US 7105724

T2-20 bromoxynil tolerance canola Z^*

T2-21 bromoxynil tolerance cotton "BXN" (Calgene)

T2-22 2,4-D tolerance maize H^*

T2-23 imidazolinone tolerance soybean N^*

A^* refers to US 4761373, US 5304732, US 5331 107, US 5718079, US 621 1438, US 621 1439 and US 6222100.

B^* refers to Tan et. al, Pest Manag. Sci 61 , 246-257 (2005).

C^* refers to imidazolinone-herbicide resistant rice plants with specific mutation of the acetohy- droxyacid synthase gene: S653N ( see e.g. US 2003/0217381 ), S654K ( see e.g. US

2003/0217381 ), A122T (see e.g. WO 04/106529) S653(At)N, S654(At)K, A122(At)T and other resistant rice plants as described in WO 2000/27182, WO 2005/20673 and WO 2001/85970 or

US patents US 5545822, US 5736629, US 5773703, US 5773704, US- 5952553, US 6274796, wherein plants with mutation S653A and A122T are most preferred.

D^* refers to WO 04/106529, WO 04/16073, WO 03/14357, WO 03/13225 and WO 03/14356.

E^* refers to US 5188642, US 4940835, US 5633435, US 5804425 and US 5627061 .

F^* refers to US 5646024 and US 5561236.

G^* refers to US 6333449, US 69331 1 1 and US 6468747.

H^* refers to US 6153401 , US 6100446, WO 2005/107437 and US 5608147.

I^* refers to Federal Register (USA), Vol. 61 , No.160, 1996, page 42581. Federal Register

(USA), Vol. 63, No.204, 1998, page 56603.

N^* refers to the herbicide tolerant soybean plants presented under the name of Cultivance on the XVI Congresso Brasileiro de Sementes, 31 st Augusta to 3^rd September 2009 at Estagao Embratel Convention Center - Curitiba/PR, Brazil

U^* "Roundup Ready Canola" (Monsanto)

V^* "Roundup Ready Corn", "Roundup Ready 2" (Monsanto), "Agrisure GT", "Agrisure GT/CB/LL", "Agrisure GT/RW",„Agrisure 3000GT" (Syngenta), "YieldGard VT Rootworm/RR2", "YieldGard VT Triple" (Monsanto)

W^* "Roundup Ready Cotton", "Roundup Ready Flex" (Monsanto)

x^* "Roundup Ready Soybean" (Monsanto), "Optimum GAT" (DuPont, Pioneer)

Y^*"Liberty Link" (Bayer),

"Herculex I", "Herculex RW", "Herculex Xtra"(Dow, Pioneer),

"Agrisure GT/CB/LL", "Agrisure CB/LL/RW" (Syngenta)

Z^*"Navigator", "Compass" (Rhone-Poulenc) In this subset of table 2, there are further preferred embodiments:

In an utmost preferred embodiment, the present invention relates to methods described above referring to afidopyropene and its individualized mixtures for the plants listed in table 2, when the plant is selected from T2-3, T2-8, T2-9, T2-10, T2-1 1 , T2-13, T2-15, T2-16, T2-17, T2-18, T2-19 and T2-23.

MIR. Methods for insecticide resistant plants

In the cases, where the cultivated plant has an arthropodicidal, preferably insecticidal, trait, it often occurs that the pest that should be combatted becomes resistant to that trait.

Resistance may be defined as 'a heritable change in the sensitivity of a pest population that is reflected in the repeated failure of a product to achieve the expected level of control when used according to the label recommendation for that pest species'. (IRAC) Resistance therefore means that the original activitiy of a pesticide against the target organisms (arthropods, insects) decreases or is even lost, due to genetic or metabolic adaptation of the target organism.

"Resistant" to an insecticide is understood to mean resistant to at least one insecticide or insecticidal trait, i.e. the insect may be resistant to only one, but also to several insecticides or insecticidal traits.

In the present context of cultivated plants with at least one insecticidal trait, the resistance is against an insecticidal effect which is due to a genetic modification of a plant (modified or trans- genie plant), which caused a resistance of the plant or crop to certain pests, especially insect pests, in susceptible insects.

This is to be understood to include plants that are by the use of recombinant DNA techniques capable to synthesize one or more insecticidal proteins, especially those mentioned herein, especially those known from the bacterial genus Bacillus, particularly from Bacillus thuringiensis, such as endotoxins, e. g. CrylA(b), CrylA(c), CrylF, CrylF(a2), CryllA(b), CrylllA, CrylllB(bl ) or Cry9c; vegetative insecticidal proteins (VIP), e. g. VIP1 , VIP2, VIP3 or VIP3A; insecticidal proteins of bacteria colonizing nematodes, for example Photorhabdus spp. or Xenorhabdus spp., and so on. Therefore, in a most preferred embodiment, the present invention relates to a method of controlling harmful insects by treating cultivated plants, parts of such plants or their locus of growth with afidopyropene or mixtures comprising afidopyropen and another insecticide, wherein the plant has at least one insecticidal trait, and wherein the harmful insects are resistant to that at least one insecticidal trait of the plant.

Preferably, the present invention relates to a method of controlling harmful insects by treating cultivated plants, parts of such plants or their locus of growth with afidopyropene or mixtures comprising afidopyropen and another insecticide, wherein the plant has at least one lepidopter- an or coleopteran trait, and wherein the harmful insects are resistant to that lepidopteran or col- eopteran insecticidal trait of the plant. In particular, the present invention relates to a method of controlling harmful insects by treating cultivated plants, parts of such plants or their locus of growth with afidopyropene or mixtures comprising afidopyropen and another insecticide, wherein the plant having the insecticidal trait corresponds to a row of table A2 or Table 3 (or further below also in table A14), and wherein the harmful insects are resistant to an insecticidal trait of the plant.

It is to be understood, that also in the mentioned methods of controlling resistant insects, afidopyropene or the mixtures comprising afidopyropen and another pesticidal active ingredient, e.g. an insecticide, may optionally be mixed with further pesticides.

Methods and uses of the invention as described herein may also involve a step of assessing whether insects are resistant to certain insecticides. This step will in general involve collecting a sample of insects from the area (e.g. crop, field, habitat) to be treated, before actually applying a afidopyropene or or mixtures comprising afidopyropen and another insecticide, and testing (for example using any suitable phenotypic, biochemical or molecular biological technique applicable) for resistance/sensitivity.

In a further preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials, or at their locus of growth with afidopyropen or mixtures of afidopyropene, wherein the mixtures comprise preferably afidopyropen and another insecticide, wherein the plant is a plant, which express at least one insecticidal toxin, preferably a toxin from Bacillus species, more preferably from Bacillus thuringiensis.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, prefera- bly seeds, with afidopyropen or mixtures comprising afidopyropen and another insecticide, wherein the plant corresponds to a row of table A.2.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table A.2. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table A.2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table A.2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table A.2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table A.2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table A.2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table A.2.

Table A2

No description transgenic plant literature / commercial

event plants

A2-1 Lepidoptera re281 -24-236 Gossypium hirsu- available, DOW Agro- sistance (DAS- tum L (cotton) Sciences LLC

24236-5)

A2-2 Lepidoptera re281 -24-236 Gossypium hirsu- available, Dow Agro- sistance x 3006-210- tum L (cotton) Sciences

23

A2-3 Lepidoptera re3006-210- Gossypium hirsu- available, DOW Agro- sistance 23 (DAS- tum L (cotton) Sciences LLC A2-4 Lepidoptera reCOT102 Gossypium hirsu- available, Syngenta sistance (SYN- tum L (cotton) Seeds, Inc.

A2-5 Lepidoptera reDAS- Gossypium hirsu- available, DOW Agro- sistance 21023-5 x tum L (cotton) Sciences LLC

DAS-24236-

A2-6 Lepidoptera reEvent-1 Gossypium hirsu- available, JK Agri Gesistance tum L (cotton) netics Ltd (India)

A2-7 Lepidoptera reMON531/75 Gossypium hirsu- available, Monsanto sistance 7/1076 tum L (cotton) Company

A2-8 Lepidoptera re15985 Gossypium hirsu- available, Monsanto sistance (MON- tum L (cotton) Company

15985-7)

A2-9 Lepidoptera re5345 Lycopersicon available, Monsanto sistance esculentum (toCompany

mato)

A2-10 Lepidoptera reMIR162 Zea mays L. available, Syngenta sistance (corn, maize) Seeds, Inc.

A2-1 1 Lepidoptera reMON89034 Zea mays L. available, Monsanto sistance (corn, maize) Company

A2-12 Corn Rootworm MIR604 Zea mays L. available, Syngenta resistance (corn, maize) Seeds, Inc.

A2-13 Corn Rootworm MON863 Zea mays L. available, Monsanto resistance (corn, maize) Company

A2-14 European Corn 176 Zea mays L. available, Syngenta Borer resistance (corn, maize) Seeds, Inc.

A2-15 European Corn MON80100 Zea mays L. available, Monsanto Borer resistance (corn, maize) Company

A2-16 European Corn MON810 Zea mays L. available, Monsanto Borer resistance (corn, maize) Company

A2-17 Colorado potato ATBT04-6, Solanum tuavailable, Monsanto beetle resistance ATBT04-27, berosum L. (potaCompany

ATBT04-30, to)

ATBT04-31 ,

ATBT04-36,

SPBT02-5,

SPBT02-7 A2-18 Colorado potato BT6, BT10, Solanum tuavailable, Monsanto beetle resistance BT12, berosum L. (potaCompany

BT16, to)

BT17,

BT18, BT23

A2-19 Colorado potato RBMT15- Solanum tuavailable, Monsanto

beetle resistance 101 , berosum L. (potaCompany

SEMT15- to)

02,

SEMT15-15

A2-20 Colorado potato RBMT21 - Solanum tuavailable, Monsanto

beetle resistance 129, berosum L. (potaCompany

RBMT21 - to)

350,

RBMT22- 082

A2-21 resistance to COT67B Gossypium hirsu- available, Syngenta

lepidopteran tum L. (Cotton) Seeds

pests

In another embodiment of the present invention, the set of methods decribed above for table A.2 concerning afidopyropen and its individualized mixtures are also to be applied, when the plant corresponds to a row of table 3.

Table 3

Literature / commer¬

No detailed description [Event] plant

cial plants

T3-1 corn rootworm resistance maize B^*

T3-2 corn borer resistance maize C^*

T3-3 western bean cutworm resistance maize D^*

T3-4 black cutworm resistance maize E^*

„Herculex I" (Dow,

T3-5 fall armyworm resistance maize Pioneer),„Herculex

Xtra" (Dow, Pioneer)

"Bollgard I" (Monsan¬

T3-6 tobacco budworm resistance cotton

to), E^*

T3-7 cotton bollworm resistance cotton E^*

T3-8 fall armyworm resistance cotton E^*

T3-9 beet armyworm resistance cotton E^*

T3-10 cabbage looper resistance cotton E^*

T3-1 1 soybean lopper resistance cotton E^* Literature / commer¬

No detailed description [Event] plant

cial plants

T3-12 pink bollworm resistance cotton E^*

T3-13 rice stemborer resistance rice A^*

T3-14 striped rice borer resistance rice A^*

T3-15 rice leaf roller resistance rice A^*

T3-16 yellow stemborer resistance rice A^*

T3-17 rice skipper resistance rice A^*

T3-18 rice caseworm resistance rice A^*

T3-19 rice cutworm resistance rice A^*

T3-20 rice armyworm resistance rice A^*

brinjal fruit and shoot borer re¬

T3-21 eggplant F^*

sistance

T3-22 cotton bollworm resistance eggplant

T3-23 tobacco hornworm resistance potato D^*

US 5349124

T3-24 lepidopteran resistance lettuce

T3-25 lepidopteran resistance soybean US 7432421

A^* refers to„Zhuxian B", WO2001021821 , Molecular Breeding, Volume 18, Number 1 / August 2006.

B^* "YieldGard corn rootworm" (Monsanto), "YieldGard Plus" (Monsanto), "YieldGard VT" (Monsanto), "Herculex RW" (Dow, Pioneer), "Herculex Rootworm" (Dow, Pioneer), "Agrisure 0CRW" (Syngenta)

C^* "YieldGard corn borer" (Monsanto),„YieldGard Plus" (Monsanto),„YieldGard VT Pro" (Monsanto), "Agrisure CB/LL" (Syngenta), "Agrisure 3000GT" (Syngenta), "Hercules I", "Hercules II" (Dow, Pioneer), "KnockOut" (Novartis),„NatureGard" (Mycogen),„Starl_ink" (Aventis)

D^*"NewLeaf" (Monsanto), "NewLeaf Y" (Monsanto), "NewLeaf Plus" (Monsanto), US6100456 E^* "Bollgard II" (Monsanto),„WideStrike" (Dow),„VipCot" (Syngenta)

F^* US 5128130, "Bt brinjal", "Dumaguete Long Purple", "Mara"

In an utmost preferred embodiment, the present invention relates to methods described above referring to afidopyropene and its individualized mixtures for the plants listed in table 3, when the plant is selected from T3-1 , T3-2, T3-5, T3-6, T3-7, T3-8, T3-9, T3-10, T3-1 1 , T3-12, T3-13, T3-14, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25.

MVR. Methods for various resitant plants MVR-1 . In a further preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials or their locus of growth with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient wherein the plant is a plant, which shows increased resistance against fungal, viral and bacterial diseases, more preferably a plant, which expresses antipathogenic substances, such as antifungal proteins, or which has systemic acquired resistance properties.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, prefera- bly seeds with afidopyropen or mixtures comprising afidopyropen and another insecticide, wherein the plant corresponds to a row of table 4.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table 4. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table 4. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table 4. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table 4. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table 4. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table 4. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table 4. Table 4

No detailed description plant Literature

T4-1 . fungal resistance apple A*, B*, C*

T4-2. fungal resistance barley A*, B*, C*

T4-3. fungal resistance banana A*, B*, C*

T4-4. fungal resistance bean B*, C*

T4-5. fungal resistance maize A*, B*, C*

T4-6. fungal resistance cotton A*, C*

T4-7. fungal resistance cucumber B*, C*

T4-8. fungal resistance grape C*

T4-9. fungal resistance oat A*, C*

T4-10. fungal resistance pepper B*, C*

T4-1 1 . fungal resistance potato A*, B*, C*

T4-12. fungal resistance rape B*, C*

T4-13. fungal resistance rice A*, B*, C*

T4-14. fungal resistance rye A*, B*, C*

T4-15. fungal resistance sorghum B*, C*

T4-16. fungal resistance soybean A*, B*, C*

T4-17. fungal resistance sugarcane B*, C*

T4-18. fungal resistance tobacco A*, B*, C*

T4-19. fungal resistance tomato A*, B*, C*

T4-20. fungal resistance wheat A*, B*, C*

T4-21 . bacterial resistance apple D*

T4-22. bacterial resistance barley D*

T4-23. bacterial resistance banana D*

T4-24. bacterial resistance bean D*

T4-25. bacterial resistance maize

T4-26. bacterial resistance cotton D*

T4-27. bacterial resistance cucumber D*

T4-28. bacterial resistance grape D*, US 6172280

T4-29. bacterial resistance oat D*

T4-30. bacterial resistance pepper D*

T4-31 . bacterial resistance potato D*

T4-32. bacterial resistance rape D*

T4-33. bacterial resistance rice D*

T4-34. bacterial resistance rye D*

T4-35. bacterial resistance sorghum D*

T4-36. bacterial resistance soybean D*

T4-37. bacterial resistance sugarcane D* No detailed description plant Literature

T4-38. bacterial resistance tobacco D^*

T4-39. bacterial resistance tomato D^*

T4-40. bacterial resistance wheat D^*

T4-41 . viral resistance apple C^*

T4-42. viral resistance barley C^*

T4-43. viral resistance banana C^*

T4-44. viral resistance bean C^*

T4-45. viral resistance maize C^*

T4-46. viral resistance cotton C^*

T4-47. viral resistance cucumber C^*

T4-48. viral resistance oat C^*

T4-49. viral resistance pepper C^*

T4-50. viral resistance potato C^*

T4-51 . viral resistance rape C^*

T4-52. viral resistance rice C^*

T4-53. viral resistance rye C^*

T4-54. viral resistance sorghum C^*

T4-55. viral resistance soybean c^*

T4-56. viral resistance sugarcane c^*

T4-57. viral resistance tobacco c^*

T4-58. viral resistance tomato c^*

T4-59. viral resistance wheat c^*

T4-60. fungal resistance potato E^*

Carica paviral resistance (PRSV) [55- available, Cornell Uni¬

T4-61 . paya (papa1/63-1 ] versity

ya)

Carica paavailable, University of

T4-62. viral resistance (PRSV) [X17-2]

paya Florida

Cucurbita available, Asgrow (USA); viral resistance (CMV, ZYMV

T4-63. pepo Seminis Vegetable Inc.

and WMV resistance), [CZW-3]

(squash) (Canada)

available, Upjohn (USA); viral resistance ( ZYMV and Cucurbita

T4-64. Seminis Vegetable Inc.

WMV resistance), [ZW20] pepo

(Canada)

available, United States

Prunus do- Department of Agricul¬

T4-65. plum pox virus resistance[C5] mestica

ture - Agricultural Re(plum tree)

search Service

A^* refers to US 5689046 and US 6020129.

B^* refers to US 6706952 and EP 1018553.

C^* refers to US 6630618. D^* refers to WO 1995/005731 and US 5648599.

E^* refers to the potato plant variety submitted for variety registration with the Community Plant Variety Office (CPVO), 3, boulevard Marechal Foch, BP 10121 , FR - 49101 Angers Cedex 02, France and having the CPVO file number 20082800

Abbreviations used: cucumber mosaiv virus = CMV, zucchini yellow mosaic virus = ZYMV watermelon mosaic virus = WMV) resistance, papaya ringspot virus = PRSV

In another embodiment of the present invention, the set of methods decribed above for table 4 concerning afidopyropen and its individualized mixtures are also to be applied, when the plant corresponds to a row of table 5 below. table 5

A^* refers to US 5689046 and US 6020129.

B^* refers to US 6706952 and EP 1018553.

C^* refers to US 6630618.

D^* refers to WO 2006/42145, US 5952485, US 5977434, WO 1999/09151 and WO 1996/22375. E^* refers to the potato plant variety submitted for variety registration with the Community Plant Variety Office (CPVO), 3, boulevard Marechal Foch, BP 10121 , FR - 49101 Angers Cedex 02, France and having the CPVO file number 20082800.

In an utmost preferred embodiment, the present invention relates to methods described above referring to afidopyropene and its individualized mixtures for the plants listed in table 5, when the plant is selected from T5-1 , T5-3, T5-4, T5-6, T5-9, T5-10, T5-12 and T5-13

MVR-2. In a further one preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials or their locus of growth with

afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant is a plant, which is tolerant to abiotic stress, preferably drought, high salinity, high light intensities, high UV irradiation, chemical pollution (such as high heavy metal concentration), low or high temperatures, limitied supply of nutrients and population stress, most preferably drought, high salinity, low temperatures and limitied supply of nitrogen.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant corresponds to a row of table 6.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table 6.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table 6.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table 6.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table 6. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table 6.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table 6. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table 6. Table 6

No detailed description plant Literature

T6-1 drought tolerance alfalfa A^*, B^*, F^*

T6-2 drought tolerance barley A^*, B^*, C^*

T6-3 drought tolerance canola A^*, B^*, F^*

T6-4 drought tolerance maize A^*, B^*, c^*, F^*

(maize)

T6-5 drought tolerance cotton A^*, B^*, c^*, F^*

T6-6 drought tolerance pomefruit A^*, B^*

T6-7 drought tolerance potato A^*, B^*, c*

T6-8 drought tolerance rapeseed A^*, B^*, c*

T6-9 drought tolerance rice A^*, B^*, c^*, F^*

T6-10 drought tolerance soybean A^*, B^*, F^*

T6-1 1 drought tolerance sugarbeet A^*, B^*

T6-12 drought tolerance sugarcane A^*, B^*, F^*

T6-13 drought tolerance sunflower A^*, B^*

T6-14 drought tolerance tomato A^*, B^*, c*

T6-15 drought tolerance wheat A^*, B^*, c^*, F^*

T6-16 tolerance to high salinity alfalfa A^*, B^*

T6-17 tolerance to high salinity barley A^*, B^*

T6-18 tolerance to high salinity canola A^*, B^*

T6-19 tolerance to high salinity maize A^*, D^*

T6-20 tolerance to high salinity cotton A^*, D^*

T6-21 tolerance to high salinity pomefruit A^*, D^*

T6-22 tolerance to high salinity potato A^*, D^*

T6-23 tolerance to high salinity rapeseed A^*, D^*

T6-24 tolerance to high salinity rice A^*, D^*,

US7034139, WO No detailed description plant Literature

2001/30990

T6-25 tolerance to high salinity soybean A^*, D^*

T6-26 tolerance to high salinity sugarbeet A^*, D^*

T6-27 tolerance to high salinity sugarcane A^*, D^*

T6-28 tolerance to high salinity sunflower A^*, D^*

T6-29 tolerance to high salinity tomato A^*, D^*

T6-30 tolerance to high salinity wheat A^*, D^*

T6-31 low temperature tolerance alfalfa A^*, E^*

T6-32 low temperature tolerance barley A^*

T6-33 low temperature tolerance canola A^*

T6-34 low temperature tolerance maize A^*, E^*

T6-35 low temperature tolerance cotton A^*, E^*

T6-36 low temperature tolerance pomefruit A^*

T6-37 low temperature tolerance potato A^*

T6-38 low temperature tolerance rapeseed A^*, E^*

T6-39 low temperature tolerance rice A^*, E^*

T6-40 low temperature tolerance soybean A^*, E^*

T6-41 low temperature tolerance sugarbeet A^*

T6-42 low temperature tolerance sugarcane A^*

T6-43 low temperature tolerance sunflower A^*

T6-44 low temperature tolerance tomato A^*

T6-45 low temperature tolerance wheat A^*, E^*

T6-46 low nitrogen supply tolerance alfalfa A^*

T6-47 low nitrogen supply tolerance barley A^*

T6-48 low nitrogen supply tolerance canola A^*

T6-49 low nitrogen supply tolerance maize A^*

T6-50 low nitrogen supply tolerance cotton A^*

T6-51 low nitrogen supply tolerance pomefruit A^*

T6-52 low nitrogen supply tolerance potato A^*

T6-53 low nitrogen supply tolerance rapeseed A^*

T6-54 low nitrogen supply tolerance rice A^*

T6-55 low nitrogen supply tolerance soybean A^*

T6-56 low nitrogen supply tolerance sugarbeet A^*

T6-57 low nitrogen supply tolerance sugarcane A^*

T6-58 low nitrogen supply tolerance sunflower A^*

T6-59 low nitrogen supply tolerance tomato A^*

T6-60 low nitrogen supply tolerance wheat A^*

A^* referes to WO 2000/04173, WO 2007/131699 and US 2008/0229448. B^* referes to WO 2005/48693.

C^* referes to WO 2007/20001 .

D^* referes to US 7256326. E^* referes to US 4731499.

F^* refers to WO 2008/002480.

In another embodiment of the present invention, the set of methods decribed above for table 6 concerning afidopyropen and its individualized mixtures are also to be applied, when the plant corresponds to a row of table 7 below.

Table 7

A^* referes to WO 2000/04173, WO 2007/131699 and US 2008/0229448.

B^* referes to WO 2005/48693.

C^* referes to WO 2007/20001 .

D^* referes to US 7256326.

E^* referes to US 4731499. In an utmost preferred embodiment, the present invention relates to methods described above referring to afidopyropene and its individualized mixtures for the plants listed in table 7, when the plant is selected from T7-1 , T7-3, T7-5, T7-6 and T7-8.

MVR-3. In a further one preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials or their locus of growth with

afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant is a plant, which shows improved maturation, preferably fruit ripening, early maturity and delayed softening.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant corresponds to a row of table 8. In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table 8.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table 8.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table 8.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table 8.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table 8.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table 8.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table 8.

Table 8

No detailed description plant/ Event Literature

T8-1 fruit ripening tomato A^*

T8-2 fruit ripening papaya US5767376, US7084321

T8-3 fruit ripening pepper B^* No detailed description plant/ Event Literature

T8-4 fruit ripening melon W01995035387

T8-5 fruit ripening strawberry W01995035387

T8-6 fruit ripening raspberry W01995035387

T8-7 fruit ripening Cucumis melo / A, B Agritope Inc.

T8-8 fruit ripening Lycopersicon esculen- Florigene Pty Ltd.

tum / 66

T8-9 fruit ripening Lycopersicon esculen- DNA Plant Technology Cortum / 1345-4 poration

T8-10 fruit ripening Lycopersicon esculen- Agritope Inc.

tum / 35 1 N

T8-1 1 fruit ripening Lycopersicon esculen- Monsanto Company

tum / 8338

T8-12 fruit ripening Lycopersicon esculen- Zeneca Seeds

tum / B, Da, F

T8-13 fruit ripening Lycopersicon esculen- Calgene Inc.

tum / FLAVR SAVR

T8-14 delayed ripening Cucumis melo / A, B available, Agritope Inc.

T8-15 delayed sofenting Lycopersicon esculen- available, Zeneca Seeds

tum/ B, Da, F

T8-16 delayed sofenting Lycopersicon esculen- available, Calgene Inc.

tum/ FLAVR SAVR

T8-17 FRA Lycopersicon esculen- available, Monsanto Comtum / 8338 pany

T8-18 FRA Lycopersicon esculen- available, DNA plant techtum / 1345-4 nology corporation

T8-19 FRA Lycopersicon esculen- available, Agritopoe Inc.

tum / 35 1 N

^*A US5952546, US 5512466, W01997/001952, W01995035387

wo1992/008798, Plant Cell. 1989; 1 (1 ): 53-63.

*B Plant Molecular Biology, Volume 50, 2002, Number 3

Abbreviations: FRA = fruit ripening alteration

Lycopersicon esculentum = tomato; Cucumis melo (melon)

MVR-4. In a further one preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials, or at their locus of growth with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant is a transgenic plant, which has modified content in comparison to wildtype plants, preferably increased vitamin content, altered oil content, nicotine reduction, increased or reduced amino acid content, protein alteration, modified starch content, enzyme alteration, altered flavo- noid content and reduced allergens (hypoallergenic plants), most preferably increased vitamin content, altered oil content, nicotine reduction, increased lysine content, amylase alteration, amylopectin alteration.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant corresponds to a row of table 9.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table 9.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table 9.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table 9.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table 9.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table 9.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table 9.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table 9. Table 9

No detailed description plant^* Literature / commercial plants

T9-1 increased Vitamin A content tomato US 6797498

T9-2 increased Vitamin A content rice "Golden rice".

Science 287, 303-305.

T9-3 increased Vitamin E content canola US 7348167, US 1 1/17071 1

(application)

T9-4 increased Vitamin E content barley US 1 1/170,71 1 (application)

T9-5 increased Vitamin E content maize US 1 1/170,71 1 (application)

T9-6 increased Vitamin E content rice US 1 1/170,71 1 (application)

T9-7 increased Vitamin E content rye US 1 1/170,71 1 (application)

T9-8 increased Vitamin E content potato US 7348167

T9-9 increased Vitamin E content soybean US 7348167

T9-10 increased Vitamin E content sunflower US 7348167

T9-1 1 increased Vitamin E content wheat US 1 1/17071 1 (application)

T9-12 decreased nicotine content tobacco US 2006/0185684, WO

2005/000352, WO

2007/064636

T9-13 amylase alteration maize "AmylaseTM"

T9-14 amylopectin alteration potato US 6784338, WO

1997/044471

T9-15 amylopectin alteration maize US 20070261 136

T9-16 modified oil content balsam pear A^*

T9-17 modified oil content canola US 5850026, US6441278,

US 5723761

T9-18 modified oil content catalpa A^*

T9-19 modified oil content cattail A^*

T9-20 modified oil content maize A^*, US 2006/0075515, US

7294759

T9-21 modified oil content cotton US 6974898, WO

2001/079499

T9-22 modified oil content grape A^*

T9-23 modified oil content rapeseed US 5723761

T9-24 modified oil content rice A^*

T9-25 modified oil content soybean A^*, US 6380462, US

6365802,

"Vistive II",„Vistsive III"

T9-26 modified oil content safflower US 6084164

T9-27 modified oil content sunflower A^*, US 6084164

T9-28 modified oil content wheat A^*

T9-29 modified oil content vernonia A^* No detailed description plant^* Literature / commercial plants

T9-30 hypoallergenic modification soybean US 6864362

T9-31 increased lysine content canola Bio/Technology 13, 577 - 582

(1995)

T9-32 increased lysine content maize „Mavera high value corn"

T9-33 increased lysine content soybean Bio/Technology 13, 577 - 582

(1995)

T9-34 altered starch content maize US 7317146, EP 1 10551 1

T9-35 altered starch content rice US 7317146, EP 1 10551 1

T9-36 altered starch content wheat EP 1 10551 1

T9-37 altered starch content barley EP 1 10551 1

T9-38 altered starch content rye EP 1 10551 1

T9-39 altered starch content oat EP 1 10551 1

T9-40 altered fllavonoid content alfalfa WO 2000/04175

T9-41 altered fllavonoid content apple WO 2000/04175

T9-42 altered fllavonoid content bean WO 2000/04175

T9-43 altered fllavonoid content maize WO 2000/04175

T9-44 altered fllavonoid content grape WO 2000/04175

T9-45 altered fllavonoid content pea WO 2000/04175

T9-46 altered fllavonoid content tomato WO 2000/04175

T9-47 increased protein content soybean „Mavera high value soybeans"

T9-48 amylopectin alteration potato B^*

T9-49 altered starch content potato C^*

T9-50 oil profile alteration/23-18-17, Brassica av.^**), Monsanto Company

23-198 napus

T9-51 oil profile alteration/46A12, Brassica av., Pioneer Hi-Bred Interna¬

46A16 napus tional Inc.

T9-52 oleic acid and linolenic acid Brassica av., Pioneer Hi-Bred Internaprofile alteration/ 45A37, napus tional Inc.

46A40

T9-53 increased shelf-life/ Carnation Dianthus av., Florigene Ltd

Moonshadow 2 caryophyllus

T9-54 linolenic acid profile alteraGlycine max av., Agriculture & Agri-Food tion/ OT96-15 L. Canada

T9-55 oil profile alteration/ G94-1 , Glycine max av., DuPont Canada Agricul¬

G94-19, G168 L. tural Products

T9-56 increased oleic acid content/ Glycine max av., Pioneer Hi-Bred Interna¬

DP-305423 L. tional Inc.

T9-57 Nicotine reduction/ Vector 21 - Nicotiana av., Vector Tobacco Inc.

41 tabacum L. No detailed description plant^* Literature / commercial

plants

T9-58 starch with increased amylo- Solanum av., BASF Plant Science

pectin content/ EH92-527-1 tuberosum

L.

T9-59 enhanced lysin level / LY038 Zea mays L. av., Monsanto Company

T9-60 modified amylase content/ Zea mays L. av., Syngenta Seeds, Inc.

Event 3272

A^* refers to US 7294759 and US 7157621 .

B^* refers to the potato plant variety submitted for variety registration with the Community Plant Variety Office (CPVO), 3, boulevard Marechal Foch, BP 10121 , FR - 49101 Angers Cedex 02, France and having the CPVO file number 20031520.

C^* refers to the potato plant variety submitted for variety registration with the Community Plant Variety Office (CPVO), 3, boulevard Marechal Foch, BP 10121 , FR - 49101 Angers Cedex 02, France and having the CPVO file number 20082534.

^*)Brassica napus (Argentine canola), Glycine max L. (soybean), Nicotiana tabacum L. (tobacco), Dianthus caryophyllus (carnation), Solanum tuberosum L. (potato), Zea mays L. (corn, maize) ^**) available

In an utmost preferred embodiment, the present invention relates to methods described above referring to afidopyropene and its individualized mixtures for the plants listed in table 9, when the plant is selected from T9-48.

In another embodiment of the present invention, the set of methods decribed above for table 9 concerning afidopyropen and its individualized mixtures are also to be applied, when the plant corresponds to a row of table 10 below. Table 10

No detailed description plant Literature / commercial plants

T10-1 increased Vitamin A content tomato US 6797498

T10-2 increased Vitamin A content rice "Golden rice".

Science 287, 303-305.

T10-3 increased Vitamin E content canola US 7348167, US 1 1/17071 1

(application)

T10-4 decreased nicotine content tobacco US 20060185684, WO

2005/000352, WO

2007/064636

T10-5 amylase alteration maize "AmylaseTM"

T10-6 amylopectin alteration potato US 6784338, WO 1997/044471

T10-7 modified oil content canola US 5850026, US 6441278, US

5723761

T10-8 modified oil content rapeseed US 5723761

T10-9 modified oil content safflower US 6084164 No detailed description plant Literature / commercial plants

T10-10 modified oil content soybean A^*, US 6380462, US 6365802;

"Vistive II",„Vistsive III"

T10-1 1 increased protein content soybean „Mavera high value soybeans"

T10-12 increased lysine content maize „Mavera high value corn"

A^* refers to US 7294759 and US 7157621 .

In an utmost preferred embodiment, the present invention relates to methods described above referring to afidopyropene and its individualized mixtures for the plants listed in table 10, when the plant is selected from T10-1 , T10-2, T10-5, T10-6, T10-10, T10-1 1 and T10-.

MVR-5. In a further one preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials, or at their locus of growth with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant is a plant, which shows improved nutrient utilization, preferably the uptake, assimilation and metabolism of nitrogen and phosphorous.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant corresponds to a row of table 1 1.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table 1 1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table 1 1.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table 1 1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table 1 1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table 1 1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table 1 1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table 1 1 .

Table 1 1

No detailed description plant Literature

T11 -1 nitrogen utilization (D^*) alfalfa A^*, B^*, F^*

T11 -2 nitrogen utilization (D^*) barley A^*, B^*

T11 -3 nitrogen utilization (D^*) canola A^*, B^*, F^*

T11 -4 nitrogen utilization (D^*) maize A^*, B^*, F^*

T11 -5 nitrogen utilization (D^*) cotton B^*, F^*

T11 -6 nitrogen utilization (D^*) potato B^*, E^*, F^*

T11 -7 nitrogen utilization (D^*) rapeseed B^*

T11 -8 nitrogen utilization (D^*) rice A^*, B^*, F^*

T11 -9 nitrogen utilization (D^*) soybean A^*, B^*, F^*

T11 -10 nitrogen utilization (D^*) sugarbeet B^*, E^*

T11 -11 nitrogen utilization (D^*) sugarcane B^*, E^*

T11 -12 nitrogen utilization (D^*) sunflower B^*

T11 -13 nitrogen utilization (D^*) tobacco p_* p_*

T11 -14 nitrogen utilization (D^*) tomato B^*, F^*

T11 -15 nitrogen utilization (D^*) wheat A^*, B^*, F^*

T11 -16 phosphorous utilization (D^*) alfalfa C^*

T11 -17 phosphorous utilization (D^*) barley C^*

T11 -18 phosphorous utilization (D^*) canola C^*

T11 -19 phosphorous utilization (D^*) maize C^*

T11 -20 phosphorous utilization (D^*) cotton C^*

T11 -21 phosphorous utilization (D^*) potato US7417181 , C^*

T11 -22 phosphorous utilization (D^*) rapeseed C^*

T11 -23 phosphorous utilization (D^*) rice C^* No detailed description plant Literature

T11 -24 phosphorous utilization (D^*) soybean C^*

T11 -25 phosphorous utilization (D^*) sugarbeet C^*

T11 -26 phosphorous utilization (D^*) sugarcane C^*

T11 -27 phosphorous utilization (D^*) sunflower C^*

T11 -28 phosphorous utilization (D^*) tomato US7417181 , C^*

T11 -29 phosphorous utilization (D^*) wheat C^*

T11 -30 low nitrogen supply tolerance canola G^*

T11 -31 low nitrogen supply tolerance maize G^*

A^* refers to US 6084153.

B^* referes to US 5955651 and US 6864405.

C^* refers to US 10/898,322 (application).

D^* the term "utilization" refers to the improved nutrient uptake, assimilation or metabolism. E^* refers to WO 1995/00991 1.

F^* refers to WO 1997/030163.

G^* referes to WO 2000/04173, WO 2007/131699 and US 2008/0229448

In an utmost preferred embodiment, the present invention relates to methods described above referring to afidopyropene and its individualized mixtures for the plants listed in table 1 1 , when the plant is selected from T1 1 -4, T1 1 -5, T1 1 -8 and T1 1 -.

MVR-6. In a further one preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants parts of such plants, plant propagation materials, or at their locus of growth with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant is a plant selected from the group consisting of cotton, fiber plants (e.g. palms) and trees, preferably a cotton plant, which produces higher quality fiber, preferably improved micronaire of the fiber, increased strength, improved staple length, improved length unifomity and color of the fibers.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant corresponds to a row of table 12.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table 12.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table 12. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table 12.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table 12.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table 12.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table 12.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table 12.

Table 12

No detailed description plant Literature

T12-1 male sterility canola US6720481

T12-2 male sterility maize A^*, B^*, C^*

T12-3 male sterility rice B^*, EP1 135982

T12-4 male sterility soybean B^*, C^*,

W01996040949

T12-5 male sterility sunflower C^*

T12-6 male sterility tomato US7345222

T12-7 male sterility wheat B^*

T12-8 male sterility2)/ MS1 , RF1 B. napus 4) AVC 1 )

=>PGS1

T12-9 male sterility2)/ MS1 , RF2 B. napus 4) AVC 1 )

=>PGS2

T12-10 male sterility2) / MS8xRF3 B. napus 4) BCS 5)

T12-1 1 male sterility3) / PHY14, B. napus 4) AVC 1 )

PHY35 No detailed description plant Literature

T12-12 male sterility3) / PHY36 B. napus 4) AVC 1 )

A^* refers to US6281348, US6399856, US7230168, US6072102. / B^* refers to WO2001062889. C^* refers to W01996040949.

1 ) Aventis Crop Science (formerly Plant Genetic Systems) / 5) Bayer CropScience (Aventis CropScience(AgrEvo) / 2) Male-sterility, fertility restoration, pollination control system displaying glufosinate herbicide tolerance. MS lines contained the barnase gene from Bacillus amylolique- faciens, RF lines contained the barstar gene from the same bacteria, and both lines contained the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces hygroscopi- cus.

3) Male sterility was via insertion of the barnase ribonuclease gene from Bacillus amyloliquefa- ciens; fertility restoration by insertion of the barstar RNase inhibitor; PPT resistance was via PPT-acetyltransferase (PAT) from Streptomyces hygroscopicus.

4) Brassica napus (Argentine Canola) For example, in one especially preferred embodiment of the present invention the plant may be a cotton plant comprising the DP 104 B2RF event ("DP 104 B2RF- A new early maturing B2RF variety" presented at 2008 Beltwide Cotton Conferences by Tom R. Speed, Richard Sheetz, Doug Shoemaker, Monsanto /Delta and Pine Land, see

http://www.monsanto.com/pdf/beltwide_08/dp104b2rf_doc.pdf.

MVR-7. In a further one preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials, or at their locus of growth with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant is resistant to antibiotics, more referably resistant to kanamycin, neomycin and ampicillin, most preferably resistant to kanamycin.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, prefera- bly seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant corresponds to a row of table 13.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table 13.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table 13.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table 13.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table 13.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table 13.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table 13.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table 13.

Table 13

A^* refers to Plant Cell Reports, 20, 2001 , 610-615. Trends in Plant Science, 1 1 , 2006, 317-319. Plant Molecular Biology, 37, 1998, 287-296. Mol Gen Genet., 257, 1998, 606-13.

B^* refers to Plant Cell Reports, 6, 1987, 333-336. In an utmost preferred embodiment, the present invention relates to methods described above referring to afidopyropene and its individualized mixtures for the plants listed in table 13, when the plant is selected from T13-2 or T13-4.

MCR. Methods for plants with combined resistance

In a further more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials, or at their locus of growth with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant is a trans- genie plant, which has two traits stacked, more preferably two or more traits selected from the group consisting of herbicide tolerance, insect resistance, fungal resistance, viral resistance, bacterial resistance, stress tolerance, maturation alteration, content modification and modified nutrient uptake, most preferably the combination of herbicide tolerance and insect resistance, two herbicide tolerances, herbicide tolerance and stress tolerance, herbicide tolerance and modified content, two herbicide tolerances and insect resistance, herbicide tolerance, insect resistance and stress tolerance, herbicide tolerance, insect resistance and modified content.

MCR-1 . For the purpose of the present invention, the cultivated plant is preferably selected from the group consisting of soybean, cotton and maize, wherein the plant has been made tolerant to the action of certain herbicides as described above. It is particularly preferred that the cultivated plant is selected from the group consisting of soybean, cotton and maize, wherein the plant has been made tolerant to the action of glyphosate herbicides.

In one embodiment, the cultivated plant is a plant, which has been made tolerant to the action of glyphosate herbicides. In addition to the tolerance to glyphosate herbicides, the plant may have been made tolerant to other herbicides and/or resistant to certain insects, and/or the plant may have been genetically modified otherwise, e.g. in terms of abiotic stress tolerance, altered growth/yield, disease resistance, modified product quality or pollination control system. In a one preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials, or at their locus of growth with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant is a glyphosate herbicide tolerant plant and corresponds to a row of table A14.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant corresponds to a row of table A14.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table A14.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table A14.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table A14.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table A14.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table A14.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table A14.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table A14.

Table A14

No traits event crop Developer / commercial plants

A14-1 Glufosinate tolerance + DAS44406-6 Glycine max L. Dow AgroSciences

Glyphosate tolerance + (soybean) LLC

2,4-D herbicide tolerance

A14-2 Glufosinate tolerance + DAS68416-4 x Glycine max L. Dow AgroSciences

Glyphosate tolerance + MON89788 (soybean) LLC

2,4-D herbicide tolerance

A14-3 Glyphosate tolerance + FG72 (FG072- Glycine max L. Bayer CropScience

Isoxaflutole tolerance 2, FG072-3) (soybean) and MS Technolo- No traits event crop Developer / commercial plants gies LLC

A14-4 Glyphosate tolerance + MON 87712 Glycine max L. Monsanto Company

Enhanced Photosynthe(soybean)

sis/Yield

A14-5 Glyphosate tolerance + MON87701 x Glycine max L. available, Monsanto

Lepidopteran resistance MON89788 (soybean) Company;

Intacta™ Roundup Ready™ 2 Pro

A14-6 Glyphosate tolerance + MON87705 Glycine max L. available, Monsanto

Modified oil/fatty acid (soybean) Company;

Vistive Gold™

A14-7 Glyphosate tolerance + MON87705 x Glycine max L. Monsanto Company

Modified oil/fatty acid MON89788 (soybean)

A14-8 Glyphosate tolerance + MON87708 Glycine max L. available, Monsanto

Dicamba tolerance (soybean) Company, Genuity®

Roundup Ready™ 2 Xtend™

A14-9 Glyphosate tolerance + MON87708 x Glycine max L. Monsanto Company

Dicamba tolerance MON89788 (soybean)

A14-10 Glyphosate tolerance + MON87769 Glycine max L. Monsanto Company

Modified oil/fatty acid (soybean)

A14-1 1 Glyphosate tolerance + MON87769 x Glycine max L. Monsanto Company

Modified oil/fatty acid MON89788 (soybean)

A14-12 Glyphosate tolerance + COT102 x Gossypium hir- available, Monsanto

Lepidopteran resistance MON15985 X sutum L. (cotCompany; + Antibiotic resistance + MON88913 ton) Bollgard® III x Visual marker Roundup Ready™

Flex™

A14-13 Glufosinate tolerance + 3006-210-23 x Gossypium hir- available, Dow

Glyphosate tolerance + 281 -24-236 x sutum L. (cotAgroSciences LLC; Lepidopteran resistance MON88913 X ton) Widestrike™ x + Antibiotic resistance COT102 Roundup Ready

Flex™ x VIPCOT™ Cotton

A14-14 Glyphosate tolerance + COT102 x Gossypium hir- available, Syngenta

Lepidopteran resistance COT67B x sutum L. (cotand Monsanto Com+ Antibiotic resistance MON88913 ton) pany; VIPCOT™

Roundup Ready Flex™ Cotton

A14-15 Glufosinate tolerance + GHB614 x Gossypium hir- available, Bayer

Glyphosate tolerance LLCotton25 sutum L. (cot- CropScience; No traits event crop Developer / commercial plants ton) GlyTol™ Liberty

Link™

A14-16 Glufosinate tolerance + GHB614 x Gossypium hir- Bayer CropScience

Glyphosate tolerance + LLCotton25 x sutum L. (cotLepidopteran resistance MON 15985 ton)

+ Antibiotic resistance +

Visual marker

A14-17 Glyphosate tolerance + GHB614 x Gossypium hir- Bayer CropScience

Lepidopteran resistance MON 15985 sutum L. (cotton)

A14-18 Glufosinate tolerance + GHB614 x Gossypium hir- available, Bayer

Glyphosate tolerance + T304-40 x sutum L. (cotCropScience;

Lepidopteran resistance GHB1 19 ton) GlyTol™ Liberty

Link™

A14-19 Glyphosate tolerance + MON1698 Gossypium hir- available, Monsanto

Antibiotic resistance sutum L. (cotCompany;

ton) Roundup Ready™

Cotton

A14-20 Glufosinate tolerance + 3272 x Bt1 1 x Zea mays L. Syngenta

Glyphosate tolerance + GA21 (corn, maize)

Lepidopteran resistance

+ Modified alpha amylase + Mannose metabolism

A14-21 Glufosinate tolerance + 3272 x BT1 1 x Zea mays L. Syngenta

Glyphosate tolerance + MIR604 X (corn, maize)

Coleopteran resistance + GA21

Lepidopteran resistance

+ Modified alpha amylase + Mannose metabolism

A14-22 Glyphosate tolerance + 3272 x GA21 Zea mays L. Syngenta

Modified alpha amylase (corn, maize)

+ Mannose metabolism

A14-23 Glyphosate tolerance + 3272 x Zea mays L. Syngenta

Coleopteran resistance + MIR604 X (corn, maize)

Modified alpha amylase GA21

+ Mannose metabolism

A14-24 Glufosinate tolerance + 5307 x Zea mays L. available, Syngenta;

Glyphosate tolerance + MIR604 x Bt1 1 (corn, maize) Agrisure® Dura- Coleopteran resistance + x TC1507 x cade™ 5122 No traits event crop Developer / commercial plants

Lepidopteran resistance GA21

+ Modified alpha amylase + Mannose metabolism

A14-25 Glufosinate tolerance + 5307 x Zea mays L. available, Syngenta;

Glyphosate tolerance + MIR604 x Bt1 1 (corn, maize) Agrisure® Dura-

Coleopteran resistance + x TC1507 x cade™ 5222

Lepidopteran resistance GA21 x

+ Modified alpha amylMIR162

ase + Mannose metabolism

A14-26 Glufosinate tolerance + 59122 x GA21 Zea mays L. Syngenta

Glyphosate tolerance + (corn, maize)

Coleopteran resistance

A14-27 Glufosinate tolerance + 59122 x Zea mays L. Syngenta

Glyphosate tolerance + MIR604 X (corn, maize)

Coleopteran resistance + GA21

Mannose metabolism

A14-28 Glufosinate tolerance + 59122 x Zea mays L. Syngenta

Glyphosate tolerance + MIR604 X (corn, maize)

Coleopteran resistance + TC1507 X

Lepidopteran resistance GA21

+ Mannose metabolism

A14-29 Glufosinate tolerance + 59122 x Zea mays L. DuPont (Pioneer Hi-

Glyphosate tolerance + MON810 X (corn, maize) Bred International

Coleopteran resistance + NK603 Inc.)

Lepidopteran resistance

A14-30 Glufosinate tolerance + 59122 x Zea mays L. Monsanto Company

Glyphosate tolerance + MON88017 (corn, maize)

Coleopteran resistance

A14-31 Glufosinate tolerance + 59122 x Zea mays L. Syngenta

Glyphosate tolerance + TC1507 X (corn, maize)

Coleopteran resistance + GA21

Lepidopteran resistance

A14-32 Glufosinate tolerance + 98140 x 59122 Zea mays L. Dow AgroSciences

Glyphosate tolerance + (corn, maize) LLC and DuPont

Sulfonylurea tolerance + (Pioneer Hi-Bred

Coleopteran resistance International Inc.)

A14-33 Glufosinate tolerance + 98140 x Zea mays L. Dow AgroSciences

Glyphosate tolerance + TC1507 (corn, maize) LLC and DuPont

Sulfonylurea tolerance + (Pioneer Hi-Bred No traits event crop Developer / commercial plants

Lepidopteran resistance International Inc.)

A14-34 Glufosinate tolerance + 98140 x Zea mays L. Dow AgroSciences

Glyphosate tolerance + TC1507 X (corn, maize) LLC and DuPont Sulfonylurea tolerance + 59122 (Pioneer Hi-Bred Coleopteran resistance + International Inc.) Lepidopteran resistance

A14-35 Glufosinate tolerance + Bt1 1 x 59122 x Zea mays L. Syngenta

Glyphosate tolerance + GA21 (corn, maize)

Coleopteran resistance +

Lepidopteran resistance

A14-36 Glufosinate tolerance + Bt1 1 x 59122 x Zea mays L. Syngenta

Glyphosate tolerance + MIR604 X (corn, maize)

Coleopteran resistance + GA21

Lepidopteran resistance

+ Mannose metabolism

A14-37 Glufosinate tolerance + BT1 1 x 59122 Zea mays L. available, Syngenta;

Glyphosate tolerance + x MIR604 X (corn, maize) Agrisure® 3122 Coleopteran resistance + TC1507 X

Lepidopteran resistance GA21

+ Mannose metabolism

A14-38 Glufosinate tolerance + Bt1 1 x 59122 x Zea mays L. Syngenta

Glyphosate tolerance + TC1507 X (corn, maize)

Coleopteran resistance + GA21

Lepidopteran resistance

A14-39 Glufosinate tolerance + Bt1 1 x MIR162 Zea mays L. available, Syngenta;

Glyphosate tolerance + x GA21 (corn, maize) Agrisure® Viptera™ Lepidopteran resistance 31 10

+ Mannose metabolism

resistance

A14-40 Glufosinate tolerance + Bt1 1 x MIR162 Zea mays L. available, Syngenta;

Glyphosate tolerance + x MIR604 X (corn, maize) Agrisure® Viptera™ Coleopteran resistance + GA21 31 1 1 , Agrisure® Lepidopteran resistance Viptera™ 4 + Mannose metabolism

A14-41 Glufosinate tolerance + Bt1 1 x MIR162 Zea mays L. available, Syngenta;

Glyphosate tolerance + x TC1507 x (corn, maize) Agrisure™ Viptera Lepidopteran resistance GA21 3220

+ Mannose metabolism

A14-42 Glufosinate tolerance + Bt1 1 X TC1507 Zea mays L. Syngenta

Glyphosate tolerance + x GA21 (corn, maize)

Lepidopteran resistance No traits event crop Developer / commercial plants

A14-43 Glyphosate tolerance + DAS40278 x Zea mays L. Dow AgroSciences

2,4-D herbicide tolerance NK603 (corn, maize) LLC

A14-44 Glyphosate tolerance HCEM485 Zea mays L. Stine Seed Farm,

(corn, maize) Inc (USA)

A14-45 Glyphosate tolerance + MIR162 X Zea mays L. Syngenta

Lepidopteran resistance GA21 (corn, maize)

+ Mannose metabolism

A14-46 Glyphosate tolerance + MIR162 X Zea mays L. Syngenta

Coleopteran resistance + MIR604 X (corn, maize)

Lepidopteran resistance GA21

+ Mannose metabolism

A14-47 Glyphosate tolerance + MIR162 X Zea mays L. Syngenta

Coleopteran resistance + TC1507 X (corn, maize)

Lepidopteran resistance GA21

+ Mannose metabolism

A14-48 Glyphosate tolerance + MIR604 X Zea mays L. available, Syngenta;

Coleopteran resistance + GA21 (corn, maize) Agrisure™ GT/RW Mannose metabolism

A14-49 Glyphosate tolerance + MIR604 X Zea mays L. DuPont (Pioneer Hi- Coleopteran resistance + NK603 (corn, maize) Bred International Mannose metabolism Inc.)

A14-50 Glyphosate tolerance + MON801 Zea mays L. Monsanto Company

Lepidopteran resistance (MON80100) (corn, maize)

+ antibiotic resistance

A14-51 Glyphosate tolerance + MON810 Zea mays L. available, Monsanto

Lepidopteran resistance (corn, maize) Company; Yield- + antibiotic resistance Gard™, Maize- Gard™

A14-52 Glyphosate tolerance + MON8741 1 Zea mays L. Monsanto Company

Coleopteran resistance (corn, maize)

A14-53 Glyphosate tolerance MON87427 Zea mays L. available, Monsanto

(corn, maize) Company; Roundup

Ready™ Maize

A14-54 Glyphosate tolerance + MON87427 x Zea mays L. Monsanto Company

Coleopteran resistance + MON89034 x (corn, maize)

Lepidopteran resistance MON88017

A14-55 Glyphosate tolerance + + MON87427 x Zea mays L. Monsanto Company

Lepidopteran resistance MON89034 x (corn, maize)

NK603

A14-56 Glufosinate tolerance + MON87427 x Zea mays L. Monsanto Company

Glyphosate tolerance + MON89034 x (corn, maize) No traits event crop Developer / commercial plants

Coleopteran resistance + TC1507 X

Lepidopteran resistance MON88017 X

59122

A14-57 Glyphosate tolerance + MON87460 x Zea mays L. Monsanto Company

Coleopteran resistance + MON89034 x (corn, maize)

Lepidopteran resistance MON88017

+ Drought stress tolerance + Antibiotic resistance

A14-58 Glyphosate tolerance + + MON87460 x Zea mays L. Monsanto Company

Lepidopteran resistance MON89034 x (corn, maize)

+ Drought stress tolerNK603

ance + Antibiotic resistance

A14-59 Glyphosate tolerance + MON87460 x Zea mays L. Monsanto Company

Drought stress tolerance NK603 (corn, maize)

+ Antibiotic resistance

A14-60 Glufosinate tolerance + MON89034 x Zea mays L. Monsanto Company

Glyphosate tolerance + 59122 x (corn, maize)

Coleopteran resistance + MON88017

Lepidopteran resistance

A14-61 Glufosinate tolerance + MON89034 x Zea mays L. Monsanto Company

Glyphosate tolerance + TC1507 X (corn, maize)

Coleopteran resistance + MON88017

Lepidopteran resistance

A14-62 Glufosinate tolerance + MON89034 x Zea mays L. available, Monsanto

Glyphosate tolerance + TC1507 X (corn, maize) Company; Genuity® Coleopteran resistance + MON88017 X SmartStax™ Lepidopteran resistance 59122

A14-63 Glufosinate tolerance + MON89034 x Zea mays L. Dow AgroSciences

Glyphosate tolerance + TC1507 X (corn, maize) LLC

Coleopteran resistance + MON88017 X

Lepidopteran resistance 59122 x

+ 2,4-D herbicide tolerDAS40278

ance

A14-64 Glufosinate tolerance + MON89034 x Zea mays L. Dow AgroSciences

Glyphosate tolerance + TC1507 X (corn, maize) LLC

Coleopteran resistance + MON88017 X

Lepidopteran resistance DAS40278

+ 2,4-D herbicide tolerance No traits event crop Developer / commercial plants

A14-65 Glufosinate tolerance + MON89034 x Zea mays L. available, Monsanto

Glyphosate tolerance + TC1507 X (corn, maize) Company and Dow Lepidopteran resistance NK603 AgroSciences LLC;

Power Core™

A14-66 Glufosinate tolerance + MON89034 x Zea mays L. Dow AgroSciences

Glyphosate tolerance + TC1507 X (corn, maize) LLC

Lepidopteran resistance NK603 x

+ 2,4-D herbicide tolerDAS40278

ance

A14-67 Glufosinate tolerance + NK603 x Zea mays L. Syngenta and MonGlyphosate tolerance + MON810 X (corn, maize) santo Company Coleopteran resistance + 41 14 x MIR

Lepidopteran resistance 604

+ Antibiotic resistance +

Mannose metabolism

A14-68 Glufosinate tolerance + NK603 x T25 Zea mays L. available, Monsanto

Glyphosate tolerance + (corn, maize) Company; Roundup Antibiotic resistance Ready™ Liberty

Link™ Maize

A14-69 Glufosinate tolerance + TC1507 x Zea mays L. available, DuPont

Glyphosate tolerance + 59122 x (corn, maize) (Pioneer Hi-Bred Coleopteran resistance + MON810 x International Inc.); Lepidopteran resistance MIR604 X Optimum™ Intrasect + Mannose metabolism NK603 Xtreme

A14-70 Glufosinate tolerance + TC1507 x Zea mays L. DuPont (Pioneer Hi- Glyphosate tolerance + MON810 x (corn, maize) Bred International Coleopteran resistance + MIR604 x Inc.)

Lepidopteran resistance NK603

+ Antibiotic resistance +

Mannose metabolism

A14-71 Glufosinate tolerance + TC1507 X Zea mays L. available, DuPont

Glyphosate tolerance + 59122 x (corn, maize) (Pioneer Hi-Bred Coleopteran resistance + MON810 X International Inc.); Lepidopteran resistance NK603 Optimum™ Intrasect

XTRA

A14-72 Glufosinate tolerance + TC1507 X Zea mays L. Monsanto Company

Glyphosate tolerance + 59122 x (corn, maize) and Dow AgroSciColeopteran resistance + MON88017 ences LLC

Lepidopteran resistance

A14-73 Glufosinate tolerance + TC1507 X Zea mays L. available, Dow

Glyphosate tolerance + 59122 x (corn, maize) AgroSciences LLC No traits event crop Developer / commercial plants

Coleopteran resistance + NK603 and DuPont (PioLepidopteran resistance neer Hi-Bred International Inc.); Hercu- lex XTRA™ RR

A14-74 Glufosinate tolerance + TC1507 X Zea mays L. DuPont (Pioneer Hi- Glyphosate tolerance + GA21 (corn, maize) Bred International Lepidopteran resistance Inc.)

A14-75 Glufosinate tolerance + TC1507 X Zea mays L. available, DuPont

Glyphosate tolerance + MIR604 X (corn, maize) (Pioneer Hi-Bred Coleopteran resistance + NK603 International Inc.); Lepidopteran resistance Optimum™ TRIsect + Mannose metabolism

A14-76 Glufosinate tolerance + TC1507 X Zea mays L. DuPont (Pioneer Hi- Glyphosate tolerance + MON810 X (corn, maize) Bred International Lepidopteran resistance MIR162 X Inc.)

+ Mannose metabolism NK603

A14-77 Glufosinate tolerance + TC1507 X Zea mays L. available, DuPont

Glyphosate tolerance + MON810 X (corn, maize) (Pioneer Hi-Bred Lepidopteran resistance NK603 International Inc.);

Optimum™ Intrasect

A14-78 Glufosinate tolerance + TC1507 X Zea mays L. Monsanto Company

Glyphosate tolerance + MON88017 (corn, maize) and Dow AgroSci- Coleopteran resistance + ences LLC

Lepidopteran resistance

A14-79 Glyphosate tolerance VCO-01981 -5 Zea mays L. Genective S.A.

(corn, maize)

In another embodiment of the present invention, the set of methods decribed above for table A14 concerning afidopyropen and its individualized mixtures are also to be applied, when the plant corresponds to a row of table 14 below.

Table 14

No detailed description / Event plant Literature / commercial

plants

T14-1 corn borer resistance + glyphosate maize "YieldGard Roundup Ready", tolerance YieldGard Roundup Ready

2" (Monsanto)

T14-2 corn borer resistance + glufosinate maize "Agrisure CB/LL" (Syntenta) tolerance

T14-3 glyphosate tolerance + corn root- maize "Yield Gard VT Root- worm resistance worm/RR2" No detailed description / Event plant Literature / commercial plants

T14-4 glyphosate tolerance + corn root- maize "Yield Gard VT Triple"

worm/corn borer resistance

T14-5 glufosinate tolerance + LPn remaize "Herculex I"

sistance (Cry1 F; western bean cutworm, corn borer, black cutworm, fall

armyworm resistance)

T14-6 glyphosate tolerance + corn root- maize "YieldGard Corn Root- worm resistance worm/Roundup Ready 2"

(Monsanto)

T14-7 glyphosate tolerance + gluphosinate maize "Herculex I / Roundup Ready tolerance + LPn resistance (Cry1 F; 2";

western bean cutworm, corn borer,

black cutworm, fall armyworm resistance)

T14-8 glyphosate tolerance + corn root- maize "YieldGard Plus / Roundup worm resistance + corn borer reReady 2" (Monsanto) sistance

T14-9 gluphosinate tolerance + LPn remaize "Agrisure GT/RW" (Syngen- sistance (Cry3A; western corn root- ta)

worm, northern corn rootworm, Mexican corn rootworm resistance)

T14-10 glyphosate tolerance + gluphosinate maize "Agrisure GT/CB/LL" (Syn- tolerance + corn borer resistance genta)

T14-1 1 glufosinate tolerance + LPn remaize "Herculex RW" (Dow, Piosistance (Cry34/35Ab1 ; western corn neer)

rootworm, northern corn rootworm,

Mexican corn rootworm resistance)

T14-12 glufosinate tolerance + LPn remaize "Herculex Xtra" (Dow, Piosistance (Cryl F + Cry34/35Ab1 ; neer)

western corn rootworm, northern

corn rootworm, Mecxican corn root- worm, western bean cutworm, corn

borer, black cutworm, fall armyworm

resistance)

T14-13 glyphosate tolerance + glufosinate maize „Herculex Quad-Stack" tolerance + corn borer resistance +

corn rootworm resistance

T14-14 glyphosate tolerance + corn root- maize "Yield Gard VT Root- worm resistance worm/RR2" No detailed description / Event plant Literature / commercial plants

T14-15 glufosinate tolerance + corn borer maize "Agrisure CB/LL/RW" (Syn- resistance (CrylAb) + LPn regenta)

sistance 3)

T14-16 glyphosate tolerance + corn borer maize "Agrisure 3000GT" (Syngen- resistance (CrylAb) + LPn reta)

sistance 3)

T14-17 glyphosate tolerance + resistance to maize „Mavera high-value corn" corn borer and corn rootworm + high (Monsanto)

lysine content

T14-18 glyphosate tolerance + ALS herbisoy-bean "Optimum GAT" (DuPont, cide tolerance (F^*) Pioneer)

T14-19 glyphosate tolerance + LP resistance soy-bean A^*, US7432421

(Bt)

T14-20 glyphosate tolerance + Dicamba soy-bean A^*, US7105724

tolerance

T14-21 glyphosate tolerance + modified oil soy-bean A^*, G^*

content

T14-22 glufosinate tolerance + modified oil soy-bean G^*, I^*

content

T14-23 glyphosate tolerance + dicamba tolecotton A^*, US7105724,

rance WO2008051633

T14-24 glufosinate tolerance + LPn recotton D^*, US5646024, US5561236 sistance

T14-25 glyphosate tolerance + LPn recotton A^*, D^*

sistance

T14-26 glufosinate tolerance + dicamba tolcotton US5646024, US5561236, erance US7105724,

WO2008051633

T14-27 glyphosate tolerance + improved cotton A^*, E^*

fiber quality

T14-28 glufosinate tolerance + improved cotton E^*, US5646024, US5561236 fiber quality

T14-29 glyphosate tolerance + drought tolcotton A^*, C^*

erance

T14-30 glyphosate tolerance + dicamba tolcotton A^*, C^*, US7105724, WO erance + drought tolerance 2008/051633

T14-31 glufosinate tolerance + insect recotton D^*, US 5646024, US

sistance (tobacco budworm, cotton 5561236

bollworm, fall armyworm, beet

armyworm, cabbage looper, soybean No detailed description / Event plant Literature / commercial plants

lopper, pink bollworm resistance)

T14-32 glyphosate tolerance + modified oil canola A^*, US 5850026, US

content 6441278, US 5723761 , WO

2005/033319

T14-33 glufosinate tolerance + modified oil canola US 5646024, US 5561236, content US 5850026, US 6441278,

US 5723761 , WO

2005/033319

T14-34 glyphosate tolerance + insect resiscanola D^*, A^*

tance

T14-35 glufosinate tolerance + insect resiscanola D^*, US 5646024, US

tance 5561236

T14-36 I Ml tolerance + Coleoptera resisrice B^*, WO 2001/021821

tance

T14-37 I Ml tolerance + LP resistance rice B^*, WO 2001/021821

T14-38 I Ml tolerance + modified oil content sun-flower Tan et. al, Pest Manag. Sci

61 , 246-257 (2005).

T14-39 Coleoptera resistance, potato H^*

+ Kanamycin resistance

T14-40 Coleoptera resistance, potato H^*

+ Kanamycin resistance + potato leaf

roll virus resistance

T14-41 Coleoptera resistance, potato H^*

+ Kanamycin resistance +potato leaf

roll virus resistance

T14-42 Glyphosate tolerance and ALH- Glycine max L. available, Pioneer Hi-Bred inhibitor tolerance / DP356043 International Inc.

T14-43 Glyphosate tolerance and ALS- Zea mays L. available, Pioneer Hi-Bred inhibitor / Event 98140tolerance International Inc.

T14-44 LP resistance and enhanced lysine Zea mays L. available, Monsanto Comcontent / MON-00810-6 x LY038 pany

T14-45 Corn root worm resistance and EPC Zea mays L. available, Monsanto Com/ MON863 x MON810 (MON-00863- pany

5, MON- 00810-6)resistance

T14-46 EPC resistance and enhanced lyZea mays L. available, Monsanto Comsine level / MON810 x LY038 pany

T14-47 Glyphosate tolerance and LPn resisGossypium available, Monsanto Comtance / MON-00531 -6 x MON- hirsutum L. (cot- pany No detailed description / Event plant Literature / commercial plants

01445-2 ton)

T14-48 Glufosinate ammonium tolerance Gossypium available, Bayer

and LPn resistance / LLCotton25 x hirsutum L. (cotCropScience (Aventis MON 15985 ton) CropScience(AgrEvo))

T14-49 Glyphosate tolerance and LPn resiGossypium available, DOW AgroScienc- stance / DAS-21023-5 x DAS- hirsutum L. (cotes LLC and Pioneer Hi-Bred 24236-5 x MON88913 (DAS-24236- ton) International Inc.

5, DAS-21023-5, MON-88913-8)

T14-50 Glyphosate tolerance and LPn resisGossypium available, Monsanto Comtance / MON 15985 x MON88913 hirsutum L. (cotpany

(MON-15985-7, MON-88913-8) ton)

T14-51 Glyphosate tolerance and LPn resisGossypium available, Monsanto Comtance/ MON-15985-7 x MON- hirsutum L. (cotpany

01445-2 ton)

T14-52 Oxynil tolerance and LPn resistance Gossypium available, Calgene Inc.

/31807/31808 hirsutum L. (cotton)

T14-53 Glyphosate tolerance and LPn resiGossypium available, DOW Ag- stance /DAS-21023-5 x DAS-24236- hirsutum L. (cotroSciences LLC

5 x MON-01445-2 ton)

T14-54 Glufosinate tolerance and Coleopte- Zea mays L. available, DOW AgroScienc- ra and LP resistance / TC1507 x es LLC and Pioneer Hi-Bred DAS-59122-7 (DAS-01507-1 , DAS- International Inc.

59122-7)

T14-55 Glyphosate tolerance and Coleopte- Zea mays L. available, Monsanto Comra and LP resistance/ MON810 x pany

MON88017

T14-56 Glyphosate tolerance and Coleopte- Zea mays L. available, Monsanto Comra and LP resistance/ MON89034 x pany

MON88017 (MON-89034-3, MON- 88017-3)

T14-57 Glyphosate tolerance and Zea mays L. available, DOW AgroScienc- Glufosinate ammonium tolerance es LLC and Pioneer Hi-Bred and Coleoptera and LP resistance/ International Inc.

DAS-59122-7 x TC1507 x NK603

T14-58 Glufosinate ammonium tolerance Zea mays L. available, Syngenta Seeds, and Coleoptera resistance/ BT1 1 x Inc.

MIR604 (SYN-BT01 1 -1 , SYN- IR604-5)

T14-59 Glyphosate tolerance and Coleop- Zea mays L. available, DOW AgroScienc- No detailed description / Event plant Literature / commercial plants

tera resistance/ DAS-59122-7 x es LLC and Pioneer Hi-Bred NK603 International Inc.

T14-60 Glyphosate tolerance and Coleop- Zea mays L. available, Syngenta Seeds, tera resistance /MIR604 x GA21 Inc.

T14-61 Glyphosate tolerance and Coleopte- Zea mays L. available, Monsanto Comra resistance / MON863 x NK603 pany

(MON-00863-5, MON-00603-6

T14-62 Glyphosate tolerance and Coleopte- Zea mays L. available, Monsanto Comra resistance and LP resistance / pany

MON863 x MON810 x NK603

T14-63 Glufosinate ammonium tolerance Zea mays L. available, DOW AgroScienc- and Corn root worm resistance es LLC and Pioneer Hi-Bred /DAS-59122-7 International Inc.

T14-64 Glyphosate tolerance and Corn root Zea mays L. available, Monsanto Comworm resistance / MON88017 pany

T14-65 Glufosinate ammonium tolerance Zea mays L. available, Dow AgroSciences and Corn root worm resistance /

DAS-59122-7

T14-66 Glufosinate ammonium tolerance Zea mays L. available, Syngenta Seeds, and EPC resistance/ BT1 1 Inc.

(X4334CBR, X4734CBR)

T14-67 Glufosinate ammonium tolerance Zea mays L. available, Aventis

and EPC resistance/ CBH-351 CropScience

T14-68 Glufosinate ammonium tolerance Zea mays L. available, Dekalb Genetics and EPC resistance/ DBT418 Corporation

T14-69 Glufosinate ammonium tolerance Zea mays L. available, Mycogen (c/o Dow and EPC resistance/ TC1507 AgroSciences); Pioneer (c/o

Dupont)

T14-70 Glyphosate tolerance and EPC resiZea mays L. available, Monsanto Comstance/ MON802 pany

T14-71 Glyphosate tolerance and EPC resi- Zea mays L. available, Pioneer Hi-Bred stance/MON809 International Inc.

T14-72 Glufosinate ammonium tolerance Zea mays L. available, Syngenta Seeds, and LPn resistance/ BT1 1 x MIR162 Inc.

(SYN-BT01 1 -1 , SYN-IR162-49

T14-73 Glufosinate ammonium tolerance Zea mays L. available, DOW Agand LPn resistance / DAS-06275-8 roSciences LLC

T14-74 Glufosinate ammonium tolerance Zea mays L. available, Syngenta Seeds, and Glyphosate tolerance and LP Inc.

resistance / BT1 1 x GA21 (SYN- BT01 1 -1 , MON-00021 -9 ) No detailed description / Event plant Literature / commercial plants

T14-75 Glufosinate ammonium tolerance Zea mays L. available, Syngenta Seeds, and Glyphosate tolerance and LP Inc.

resistance/ BT1 1 x MIR604 x GA21

(SYN-BT01 1 -1 , SYN-IR604-5,

MON-00021 -9)

T14-76 Glufosinate ammonium tolerance Zea mays L. available, DOW Ag- and Glyphosate tolerance and LP roSciences LLC

resistance/ TC1507 x NK603 (DAS- 01507-1 x 00603-6)MON-

T14-77 Glyphosate tolerance and LPn resiZea mays L. available, Monsanto Comstance/ GA21 x MON810 pany

T14-78 Glyphosate tolerance and LPn resisZea mays L. available, Monsanto Comtance/ MON89034 x NK603 (MON- pany

89034-3, MON- 00603-6)

T14-79 Glyphosate tolerance and LPn resisZea mays L. available, Monsanto Comtance/ NK603 x MON810 (MON- pany

00603-6, MON-00810-6)

T14-80 Glufosinate ammonium tolerance Zea mays L. available, Bayer

and LPn resistance/ T25 x MON810 CropScience (Aventis (ACS-ZM003-2, MON-00810-6) CropScience(AgrEvo))

T14-81 Gluphosinate tolerance and male Brassica napus available, Bayer

sterility/ MS1 , RF1 (PGS1 ) CropScience (Aventis

CropScience(AgrEvo))

T14-82 Gluphosinate tolerance and male Brassica napus available, Aventis Crop- sterility/ MS1 , RF2 (PGS2) Science (formerly Plant Genetic Systems)

T14-83 Gluphosinate tolerance and male Brassica napus available, Bayer

sterility/ MS8xRF3 CropScience (Aventis

CropScience(AgrEvo))

T14-84 Gluphosinate tolerance and male Zea mays L. available, Bayer

sterility/ MS3 (ACS-ZM001 -9) CropScience (Aventis

CropScience(AgrEvo))

T14-85 Gluphosinate tolerance and male Zea mays L. available, Bayer

sterility/ MS6 (ACS-ZM005-4) CropScience (Aventis

CropScience(AgrEvo))

T14-86 glyphosate tolerance and high oleic Glycine max L. available, Pioneer Hi-Bred acid content/305423 x 40-3-2

T14-87 coloration and sulfonylurea herbicide D.caryophyllus available, Florigene Pty Lt tolerance/4, 1 1 , 15, 16

T14-88 coloration and sulfonylurea herbicide D.caryophyllus available, Florigene Pty Lt tolerance / 959A, 988A, , 1363A, No detailed description / Event plant Literature / commercial

plants

1400A 1226A, 1351 A

T14-89 Increased shelf-life and sulfonylurea D.caryophyllus available, Florigene Pty Lt herbicide tolerance / 66

^*) Glycine max L. (soybean), Zea mays L. (corn, maize), Brassica napus (Argentine canola), D. caryophyllus = Dianthus caryophyllus (carnation)

^**) European corn borer = EPC, Lepidoptera LP, Lepidopteran LPn, Glyphosate tolerance = GLY-T

A^* refers to US 5188642, US 4940835, US 5633435, US 5804425 and US 5627061 .

B^* refers to imidazolinone-herbicide resistant rice plants with specific mutation of the acetohy- droxyacid synthase gene: S653N (see e.g. US 2003/0217381 ), S654K (see e.g. US

2003/0217381 ), A122T (see e.g. WO 2004/106529) S653(At)N, S654(At)K, A122(At)T and other resistant rice plants as described in WO 2000/27182, WO 2005/20673 and WO 2001/85970 or US patents US 5545822, US 5736629, US 5773703, US 5773704, US- 5952553, US

6274796, wherein plants with mutation S653A and A122T are most preferred.

C^* referes to WO 2000/04173, WO 2007/131699, US 20080229448 and WO 2005/48693.

D^* refers to WO 1993/07278 and WO 1995/34656.

E^* refers to WO 1996/26639, US 7329802, US 6472588 and WO 2001/17333.

F^* refers to sulfonylurea and imidazolinone herbicides, such as imazamox, imazethapyr, ima- zaquin, chlorimuron, flumetsulam, cloransulam, diclosulam and thifensulfuron.

G^* refers to US 6380462, US 6365802, US 7294759 and US 7157621 .

H^* refers to Plant Cell Reports, 20, 2001 , 610-615. Trends in Plant Science, 1 1 , 2006, 317-319. Plant Molecular Biology, 37, 1998, 287-296. Mol Gen Genet., 257, 1998, 606-13. Federal Reg- ister (USA), Vol.60, No.1 13, 1995, page 31 139. Federal Register (USA), Vol.67, No.226, 2002, page 70392. Federal Register (USA), Vol.63, No.88, 1998, page 25194. Federal Register (USA), Vol.60, No.141 , 1995, page 37870. Canadian Food Inspection Agency, FD/OFB-095- 264-A, October 1999, FD/OFB-099-127-A, October 1999.

I^* refers to Federal Register (USA), Vol. 61 , No.160, 1996, page 42581 . Federal Register (USA), Vol. 63, No.204, 1998, page 56603.

3) (Cry3A; western corn rootworm, northern corn rootworm, Mexican corn rootworm resistance)

In an utmost preferred embodiment, the present invention relates to methods described above referring to afidopyropene and its individualized mixtures for the plants listed in table 14, when the plant is selected from T14-1 , T14-2, T14-3, T14-4, T14-5, T14-6, T14-7, T14-8, T14-9, T14- 10, T14-1 1 , T14-12, T14-13, T14-14, T14-15, T14-17, T14-23, T14-24, T14-25, T14-26, T14-31 , T14-36 and T14-37.

In addition to the methods and usese described above, afidopyropen and its individualized mix- tures may also be applied to crop plants such as soybean, rice, corn (maize), cotton, sugar beet and canola, when the plant corresponds to a row of table B14 below. Table B14

No trait type seed traits event crop developer commercial product

B14-1 HT HPPD SYHT0H2 Soybean Syngenta N/A

B14-2 Y&S DT MYDT09Y Corn Syngenta N/A

B14-3 Y&S NUE N/A Corn Syngenta Agrisure

B14-4 IR RW³ N/A Corn Syngenta N/A

B14-5 IR BL N/A Rice Syngenta N/A

B14-6 IR SCN N/A Soybean Syngenta N/A

B14-7 FR Disease N/A Soybean Syngenta N/A

resistance

B14-8 Y&S Abiotic N/A Corn Syngenta N/A

stress

B14-9 IR BL MIR162 Corn Syngenta Agrisure™

Viptera™

B14-10 HT/IR BL-GT-LL Bt1 1 x Pat x Corn Syngenta Agrisure® VipMIR162 x GA21 tera™ 31 10

B14-1 1 HT/IR CB-BL- Bt1 1 x Pat x Corn Syngenta Agrisure™

RW -GT- MIR162 X Viptera 3220

LL TC1507 x GA21 E-Z Refuge

B14-12 ST CA 3272 Corn Syngenta Enogen™

B14-13 IR RW² 5307 Corn Syngenta Agrisure® Du- racade™

B14-14 HT/IR BL-CB- 5307 x MIR604 Corn Syngenta Agrisure® Du- Col-RW - x BtHxPat x racade™ 5222 RW²-LL- TC1507 x GA21

GT x MIR162

B14-15 IR BL COT102 Cotton Syngenta VIPCOT™

B14-16 HT GT 73496 Canola Dupont Optimum®

Gly™

B14-17 HT GT 61061 Canola Dupont N/A

B14-18 HT/IR CB-BL- DP41 14 Corn Dupont Herculex 1 +

RW-LL

B14-19 HT/IR CB-BL- DP41 14 Corn Dupont Herculex RW

RW-LL

B 14-20 HT/IR CB-BL-LL Corn Dupont N/A

B14-21 IR Aphid Soybean Dupont N/A

B 14-22 FR FR Soybean Dupont N/A

B 14-23 HT Corn Dupont N/A

B 14-24 HT GT-SU N/A Rice Dupont N/A No trait type seed traits event crop developer commercial product

B 14-25 HT SU-GT- 98140 X TC1507 Corn Dupont N/A

LL-CB- x 59122

RW-BL

B 14-26 Y&S NUE Corn Dupont N/A

B 14-27 IR BL N/A Rice Dupont N/A

B 14-28 Y&S DT-NUE Corn Dupont N/A

B 14-29 HT/IR DP-032218-9 Dupont N/A

B 14-30 HT/IR ECB;FAW DP-033121 -3 Dupont N/A

CEW GIu

B14-31 Y&S DT Native Corn Dupont Aquamax II™

B 14-32 HT/IR RW-LL- 59122 x NK603 Corn Dupont Roundup

GT Ready™ 2

B 14-33 HT/IR CB-RW- TC1507 X Corn Dupont Optimum™

LL-GT 59122x Intrasect

MON810 X XTRA NK603

B 14-34 HT/IR CB-RW2- TC1507 X 59122 Corn Dupont Optimum™

GT-LL x MON810 X Intrasect

MIR604 X Xtreme NK603

B 14-35 HT/ST GT-Oleic DP305423 Soybean Dupont Plenish™

B 14-36 Y&S DT Native Corn Dupont AQUAmax®

B 14-37 HT 2,4D-LL DAS68416-4 or Soybean Dow Enlist™

"416"

B 14-38 HT 2,4D-LL- DAS68416-4 x Soybean Dow Enlist™RR2

GT MON89788

B 14-39 HT/IR LL-BL+2 DAS81419 Soybean Dow Enlist™ E3 +

2Bt

B 14-40 HT 2,4D-LL- DAS44406-6 Soybean Dow Enlist™ E3

GT

B14-41 HT/IR 2,4D-LL- DAS44406-6 x Soybean Dow N/A

GT-BL+2 DAS81419

B 14-42 HT 2,4D DAS40278 Corn Dow Enlist™

B 14-43 HT/IR LL-GT-BL 3006-210-23 x Cotton Dow Widestrike™

281 -24-236 x III WidMON88913 X estrike™ X COT102 RRFlex™ x

VIPCOT™

B 14-44 HT/IR LL-GT- 98140 x Corn Dow N/A

SU-Col DAS 1507 No trait type seed traits event crop developer commercial product

B 14-45 HT GT-2,4D DAS40278 x Corn Dow Enlist RR

NK603

B 14-46 HT/IR CB-BL+5- MON89034 x Corn Dow PowerCore™

GT-LL- TC1507 X

CW MON88017 X

DAS59122 X

DAS40278

B 14-47 HT/IR 2,4D-GT- MON89034 x Corn Dow SmartStax

BL-Col- DAS 1507 x Enlist

CW MON88017 X

59122 x

DAS40278

B 14-48 HT/IR LL-BL 281 -24-236 x Cotton Dow WideStrike™!

3006-210-23

(MXB-13)

B 14-49 HT IR HT Stack MON87427 x Corn Monsanto SmartStax Pro

(Gly+Glu) MON89034 x

I R Stack TC1507 X

(Coleo+Le MON88017 X

Pi) 59122

B 14-50 HT DR MON87460 x Corn Monsanto Droughtgard™

NK603

B14-51 HT IR MON87701 x Soybean Monsanto Intacta™

MON89788 Roundup

Ready™ 2 Pro

B 14-52 HT OIL HT OIL MON87705 Soybean Monsanto Vistive Gold™

Oleic

(Food and

Bio based

lubricants)

B 14-53 HT GT200 (RT200) Canola Monsanto Roundup

Ready™ Canola

B 14-54 HT GT GT73 (RT73) Canola Monsanto Roundup

Ready™ Canola

B 14-55 HT IR GT MON88302 Canola Monsanto TruFlex™

Roundup Ready™ Canola No trait type seed traits event crop developer commercial product

B 14-56 HT IR COT102 x Cotton Monsanto Bollgard® III x

MON15985 X Roundup

MON88913 Ready™

Flex™

B 14-57 HT IR GT-BL 31707 Cotton Monsanto BXN™ Plus

Bollgard™

Cotton

B 14-58 HT IR GT-BL 31803 Cotton Monsanto BXN™ Plus

Bollgard™

Cotton

B 14-59 HT IR GT-BL 31807 x 31808 Cotton Monsanto not available

B 14-60 HT IR GT-BL 31807 Cotton Monsanto BXN™ Plus

Bollgard™

Cotton

B14-61 HT IR GT-BL 31808 Cotton Monsanto BXN™ Plus

Bollgard™

Cotton

B 14-62 HT GT-BL 42317 Cotton Monsanto BXN™ Plus

Bollgard™

Cotton

B 14-63 HT GT BXN1021 1 Cotton Monsanto BXN™ Cotton

(1021 1 )

B 14-64 HT GT BXN10215 Cotton Monsanto BXN™ Cotton

(10215)

B 14-65 HT GT BXN 10222 Cotton Monsanto BXN™ Cotton

(10222)

B 14-66 HT IR GT BXN 10224 Cotton Monsanto BXN™ Cotton

(10224)

B 14-67 HT GLU- COT102 x Cotton Monsanto Bollgard® III

(Glu+Dic Dicamba MON 15985

amba)

B 14-68 IR MON 88701 -3 Cotton Monsanto not available

B 14-69 HT MON1076 Cotton Monsanto Bollgard™

Cotton

B 14-70 IR MON1445 Cotton Monsanto Roundup

Stacks Ready™ Cot(Lepi) ton

B14-71 IR HT MON 15985 Cotton Monsanto Bollgard II™

Cotton No trait type seed traits event crop developer commercial product

B 14-72 HT MON15985 X Cotton Monsanto Roundup

MON1445 Ready™ Boll- gard II™ Cotton

B 14-73 IR MON1698 Cotton Monsanto Roundup

Ready™ Cotton

B 14-74 HT IR MON531 Cotton Monsanto Bollgard™

Cotton,

Ingard™

B 14-75 IR MON531 x Cotton Monsanto Roundup

MON1445 Ready™ Bollgard™ Cotton

B 14-76 HT MON757 Cotton Monsanto Bollgard™

Cotton

B 14-77 HT IR MON88913 Cotton Monsanto Roundup

Ready™ Flex™ Cotton

B 14-78 HT (Glu) MON88913 X Cotton Monsanto Roundup

IR MON 15985 Ready™

Flex™ Bollgard II™ Cotton

B 14-79 HT DBT418 Corn Monsanto Bt Xtra™ Maize

B 14-80 HT (Gly) DLL25 (B16) Corn Monsanto not available

B14-81 HT IR GA21 Corn Monsanto Roundup

Ready™ Maize, Agri- sure™GT

B 14-82 HT IR GA21 x Corn Monsanto Roundup

MON810 Ready™ Yiel- dGard™ maize

B 14-83 HT IR MON801 Corn Monsanto not available

(MON80100)

B 14-84 HT IR MON802 Corn Monsanto not available

B 14-85 HT IR MON810 Corn Monsanto YieldGard™,

Stack MaizeGard™ (Coleo+L

epi) No trait type seed traits event crop developer commercial product

B 14-86 HT MON810 X Corn Monsanto YieldGard™

MON88017 VT Triple

B 14-87 IR MON832 Corn Monsanto Roundup

Ready™ Maize

B 14-88 I R Stack MON863 Corn Monsanto YieldGard™

(Coleo+L Rootworm epi) RW,

MaxGard™

B 14-89 HT IR MON863 x Corn Monsanto YieldGard™

MON810 Plus

B 14-90 HT IR MON863 x Corn Monsanto YieldGard™

(Coleo) MON810 X Plus with RR

NK603

B14-91 HT IR MON863 x Corn Monsanto YieldGard™

Western NK603 RW + RR Corn

Root- worm

RNAi)

B 14-92 HT MON8741 1 Corn Monsanto Not available

B 14-93 HT IR MON87427 Corn Monsanto Roundup

Ready™ Maize

B 14-94 HT IR MON87427 x Corn Monsanto not available

MON89034 x

MON88017

B 14-95 HT Stack MON87427 x Corn Monsanto not available

(Gly+Glu MON89034 x

) IR NK603

Stack

(Coleo+L

epi)

B 14-96 HT Stack MON87427 x Corn Monsanto SmartStax Pro

(Gly+Glu MON89034 x

) IR TC1507 X

Stack MON88017 X

(Coleo+L 59122

epi)+DR No trait type seed traits event crop developer commercial product

B 14-97 HT Stack MON87460 x Corn Monsanto not available

(Gly+Glu MON89034 x

) IR MON88017

Stack

(Coleo+L

epi)+DR

B 14-98 HT DR MON87460 x Corn Monsanto not available

MON89034 x

NK603

B 14-99 HT IR MON87460 x Corn Monsanto Droughtgard™

NK603

B14-100 IR MON88017 Corn Monsanto YieldGard™

VT™ Root- worm™ RR2

B14-101 HT IR MON89034 Corn Monsanto YieldGard™

VT Pro™

B14-102 HT IR MON89034 x Corn Monsanto Genuity® VT

MON88017 Triple Pro™

B14-103 HT MON89034 x Corn Monsanto Genuity® VT

NK603 Double Pro™

B14-104 HT IR NK603 Corn Monsanto Roundup

Ready™ 2 Maize

B14-105 HT (Gly NK603 x Corn Monsanto YieldGard™

+ Glu) MON810 CB + RR

B14-106 HT NK603 x T25 Corn Monsanto Roundup

Ready™ Liberty Link™ Maize

B14-107 HT Y GTS 40-3-2 (40- Soybean Monsanto Roundup

3-2) Ready™ soybean

B14-108 IR MON 87712 Soybean Monsanto Not available

B14-109 HT IR MON87701 Soybean Monsanto not available

B14-1 10 HT OIL MON87701 x Soybean Monsanto Intacta™

Oleic MON89788 Roundup

Ready™ 2 Pro

B14-1 1 1 HT OIL MON87705 Soybean Monsanto Vistive Gold™

Oleic +

IR No trait type seed traits event crop developer commercial product

B14-1 12 HT (Gly MON87705 x Soybean Monsanto not available

+ Di- MON89788

camba)

B14-1 13 HT (Gly MON87708 Soybean Monsanto Genuity®

+ Di- Roundup camba) Ready™ 2

Xtend™

B14-1 14 IR MON87708 x Soybean Monsanto not available

MON89788

B14-1 15 HT OIL MON87751 Soybean Monsanto not available

SDA

B14-1 16 HT OIL MON87769 Soybean Monsanto not available

SDA

B14-1 17 HT MON87769 x Soybean Monsanto not available

MON89788

B14-1 18 HT MON89788 Soybean Monsanto Genuity®

Roundup Ready 2 Yield™

B14-1 19 HT H7-1 Sugar Monsanto Roundup

beet Ready™ sugar beet

B14-120 HT MON71800 Wheat Monsanto Roundup

Ready™ wheat

B14-121 HT GT-LL-PC MS8 x RF3 x Hybrid Bayer InVigor Liberty

GT73 (RT73) Canola Link + GT

B14-122 HT GT-LL MON88302 x Canola Bayer TruFlex™

MS8 x RF3 x Roundup GT73 (RT73) Ready™

Canola

B14-123 HT/ST GT-Oleic Canola Bayer InVigor™ Canola Healthy Oil

B14-124 HT/Y&S GT-Y Canola Bayer N/A

B14-125 HT/IR GT-LL-BL Canola Bayer N/A

B14-126 IR BL COT102 Cotton Bayer N/A

B14-127 HT/IR LL -BL GHB614 x LL25 Cotton Bayer N/A

x MON15985 No trait type seed traits event crop developer commercial product

B14-128 HT LL Arize® 644 Rice Bayer N/A

Hybrid (Native)

+ ACS-OS002-5

B14-129 IR BL Arize® 644 HybRice Bayer N/A

rid (Native) + BL

B14-130 Y&S Y Arize® 644 HyRice Bayer N/A

brid (Native) +

Y&S

B14-131 Y&S Y Rice Bayer N/A

B14-132 HT HPPD SYT0H2 Soybean Bayer N/A

B14-133 HT GT-HPPD Soybean Bayer N/A

B14-134 IR SCN Soybean Bayer N/A

B14-135 HT GT GHB614 Cotton Bayer GlyTol™ Cotton

B14-136 HT/IR LL-GT-BL GHB614 x Cotton Bayer GlyTol™ x

T304-40 x TwinLink GHB1 19

B14-137 HT/IR LL-BL LLCotton25 x Cotton Bayer Fibermax™

MON-15985 Liberty Link™

Bollgard II™

B14-138 HT LL MS8/RF3 Canola Bayer InVigor™ Canola

B14-139 HT LL-GT GHB614 x Cotton Bayer GlyTol™ x

LL25Cotton Liberty Link™

B14-140 HT LL HCN92 Topas Canola Bayer Liberty Link™

19/2 Innovator™

Topas

B14-141 HT LL LLCOtton25 Cotton Bayer Fibermax™

Liberty Link™

B14-142 Y&S Y native Rice Bayer Arize® 644

Hybrid

In the above table B14, the abbreviations are as follows

Trait type = trait category

Seed trait = trait sub-category

Trait types:

HT Herbicide Tolerance

IR Insect resistance (including Nematodes)

FR Fungal resistance

Y&S Yield and Stress

ST Specialty Trait (includes Feed, Food, Quality) DT Drought tolerance

CA Corn amylase

SCN Soybean Cyst Nematode resistance

BL broad spectrum resistance against lepidopterans (above ground worms)

GT Glyphosate tolerance

LL Liberty Link, confers glufosinate tolerance (trait origin is Bayer)

Col resistance against Coleopterans (beetles)

SU Sulfonylurea tolerance

Oleic High oleic acid levels

Seed traits:

HT Herbicide Tolerance

2,4D resistance against 2,4-D Choline

BL broad spectrum resistance against lepidopterans (above ground worms) BL Broad spectrum resistance against Lepidopterans (above-ground worms)

BL+2/5 BL enhanced by 2 or 5 new targets

CA Corn amylase

CB corn borer resistance

Col resistance against Coleopterans (beetles)

DHA Docosahexaenoic acid, Omega 3 fatty acid

DT Drought tolerance

FR Fungal resistance

Germp germplasm

GT Glyphosate tolerance

HPPD HPPD inhibitor resistance (Mesotrione)

HT Herbicide tolerance

IR Insect resistance (including Nematodes)

LL Liberty Link confers glufosinate tolerance (trait origin is Bayer)

NUE Nitrogen Use Efficiency

Oleic High oleic acid levels

PC Pollination control male sterility system

RW Root worm resistance

RW1 Root worm resistance, mode of action 1

RW2 Root worm resistance, mode of action 2

RW3 Root worm resistance mode of action 3

SCN Soybean Cyst Nematode resistance

SDA increased SDA Omega-3 levels

SR Stalk rot resistance

ST Specialty Trait (includes Feed, Food, Quality)

SU Sulfonylurea tolerance

Y&S Yield and Stress MCR-2. Insect resistance, in particular lepidopteran resistance is of growing importance in GMO crops. Furthermore, it has been found that insects often become resistant to the crops, which have been modified in terms of insect resistance. It has been found that afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, preferably an insecticide, are particularly suitable for combating insects, which have become resistant to the crops, which have been modified in terms of insect resistance. In particular, afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, preferably an insecticide, may advantageously be applied in soybeans, which habe been made resistant to insects. In one embodiment, the cultivated plant is soybean, which has been made resistant to lepidop- eran insects. In addition to the resistance to lepidoperan insects, the soybean may have been made tolerant to certain herbicides and/or resistant to other insects, and/or the soybean may have been genetically modified otherwise, e.g. in terms of abiotic stress tolerance, altered growth/yield, disease resistance, modified product quality or pollination control system.

In a particularly preferred embodiment, the present invention therefore relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or their locus of growth with with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant is a lepidopteran insect resistant soybean and corresponds to a row of table B2.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant corresponds to a row of table B2.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table B2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table B2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table B2. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table B2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table B2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table B2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table B2.

Table B2

MCR-3. Lepidopteran resistance of soybeans is typically achieved by introducing a gene selected from the group consisting of: crylAc (gene source: Bacillus thuringiensis subsp. Kurstaki strain HD73), cry1 F (gene source: Bacillus thuringiensis var. aizawai), cry1A.105 (gene source: Bacillus thuringiensis subsp. Kumamotoensis), cry2Ab2 (gene source: Bacillus thuringiensis subsp. Kumamotoensis), and combinations thereof. In addition, the soybeans may be modified e.g. in terms of herbicide tolerance by introducing a suitable gene such as pat (gene source: Streptomyces viridochromogenes), which provides glufosinate tolerance or cp4 epsps

(aroA:CP4) (gene source: Agrobacterium tumefaciens strain CP4), which provides glyphosate tolerance. Preferably, the soybeans are additionally modified in terms of glyphosate tolerance by introducing the gene cp4 epsps (aroA:CP4).

In a one preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants, plant propagation materials, or at their locus of growth with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant is a lepidop- teran insect resistant soybean, which has been modified by introducing at least one gene or at least one gene combination, which corresponds to a row of table C2.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant corresponds to a row of table C2.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table C2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table C2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table C2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table C2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table C2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table C2. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table C2.

Table C2

MGM Methods for plants with specific expressed genetic modicifaction MGM-1 . The present invention also relates to a method for controlling pests and/or increasing the plant health of a cultivated plant as compared to the respective non-modified control plant, comprising the application of afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, to a plant with at least one modification, parts of such plant, plant propagation material, or at its locus of growth, wherein the cultivated plant is

(i) Gossypium hirsutum L. (cotton) or Zea mays L. (maize) and comprises at least one gene selected from the group consisting of dvsnf7, cry1A, cry1Ab-Ac, cry1 C, cry2Ab2, cry2Ae, mocryl F; or

(ii) Glycine max L. (soybean), Triticum aestivum (wheat) or Oryza sativa L. (rice) and comprises at least one gene selected from the group consisting of cry34Ab1 , cry35 Ab1 , cry3A, cry3Bb1 , dvsnf7, mcry3A, cry1A, cry1A.105, crylAb, cry1Ab-Ac, crylAc, cryl C, cry1 F, cry1 Fa2, cry2Ab2, cry2Ae, cry9c, mocryl F, pinll, vip3A(a), vip3Aa20.

Prefarably, present invention also relates to a method for controlling pests and/or increasing the plant health of a cultivated plant as compared to the respective non-modified control plant, comprising the application of afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, to a plant with at least one modification, parts of such plant, plant propagation material, or at its locus of growth, wherein the cultivated plant is modified by at least one gene according to one row of table D2.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant corresponds to a row of table D2.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant corresponds to a row of table D2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant corresponds to a row of table D2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant corresponds to a row of table D2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant corresponds to a row of table D2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant corresponds to a row of table D2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant corresponds to a row of table D2.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant corresponds to a row of table D2.

Table D2 No. crop gene

No. crop gene D2-41 wheat mcry3A

D2-1 cotton dvsnf7 D2-42 wheat cry1A

D2-2 cotton cry1A D2-43 wheat cry1A.105

D2-3 cotton cry1Ab-Ac D2-44 wheat crylAb

D2-4 cotton cry1 C D2-45 wheat cry1Ab-Ac

D2-5 cotton cry2Ab2 D2-46 wheat cry 1 Ac

D2-6 cotton cry2Ae D2-47 wheat cry1 C

D2-7 cotton mocryl F D2-48 wheat cry1 F

D2-8 maize dvsnf7 D2-49 wheat cry1 Fa2

D2-9 maize cry1A D2-50 wheat cry2Ab2

D2-10 maize cry1Ab-Ac D2-51 wheat cry2Ae

D2-1 1 maize cry1 C D2-52 wheat cry9c

D2-12 maize cry2Ab2 D2-53 wheat mocryl F

D2-13 maize cry2Ae D2-54 wheat pinll

D2-14 maize mocryl F D2-55 wheat vip3A(a)

D2-15 soybean cry34Ab1 D2-56 wheat vip3Aa20

D2-16 soybean cry35 Ab1 D2-57 rice cry34Ab1

D2-17 soybean cry3A D2-58 rice cry35 Ab1

D2-18 soybean cry3Bb1 D2-59 rice cry3A

D2-19 soybean dvsnf7 D2-60 rice cry3Bb1

D2-20 soybean mcry3A D2-61 rice dvsnf7

D2-21 soybean cry1A D2-62 rice mcry3A

D2-22 soybean cry1A.105 D2-63 rice cry1A

D2-23 soybean crylAb D2-64 rice cry1A.105

D2-24 soybean cry1Ab-Ac D2-65 rice crylAb

D2-25 soybean cry 1 Ac D2-66 rice cry1Ab-Ac

D2-26 soybean cry1 C D2-67 rice cry 1 Ac

D2-27 soybean cry1 F D2-68 rice cry1 C

D2-28 soybean cry1 Fa2 D2-69 rice cry1 F

D2-29 soybean cry2Ab2 D2-70 rice cry1 Fa2

D2-30 soybean cry2Ae D2-71 rice cry2Ab2

D2-31 soybean cry9c D2-72 rice cry2Ae

D2-32 soybean mocryl F D2-73 rice cry9c

D2-33 soybean pinll D2-74 rice mocryl F

D2-34 soybean vip3A(a) D2-75 rice pinll

D2-35 soybean vip3Aa20 D2-76 rice vip3A(a)

D2-36 wheat cry34Ab1 D2-77 rice vip3Aa20

D2-37 wheat cry35 Ab1

D2-38 wheat cry3A

D2-39 wheat cry3Bb1

D2-40 wheat dvsnf7 MGM-2. Further preferred embodiments of the invention are those methods of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth afidopyropen or mixtures of afidopyropene with another pesti- cidal active ingredient, wherein the plant expresses one or more genes selected from CP4 ep- sps, pat, bar, Cry1 Ab, Cry1 Ac, Cry3Bb1 , Cry2Ab, Cry1 F, Cry34Ab1 and Cry35Ab1 .

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, , wherein the plant expresses one or more genes selected from CP4 epsps, pat, bar, CrylAb, CrylAc, Cry3Bb1 , Cry2Ab, Cry1 F, Cry34Ab1 and Cry35Ab1 .

In such method, the afidopyropen is preferably applied in combination with abamectin, dinotefuran, ethiprole und fipronil. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant expresses one or more genes selected from CP4 epsps, pat, bar, CrylAb, CrylAc, Cry3Bb1 , Cry2Ab, Cry1 F, Cry34Ab1 and Cry35Ab1.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant expresses one or more genes selected from CP4 epsps, pat, bar, CrylAb, CrylAc, Cry3Bb1 , Cry2Ab, Cry1 F, Cry34Ab1 and Cry35Ab1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant expresses one or more genes selected from CP4 epsps, pat, bar, CrylAb, CrylAc, Cry3Bb1 , Cry2Ab, Cry1 F, Cry34Ab1 and Cry35Ab1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant expresses one or more genes selected from CP4 epsps, pat, bar, CrylAb, CrylAc, Cry3Bb1 , Cry2Ab, Cry1 F, Cry34Ab1 and Cry35Ab1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant expresses one or more genes selected from CP4 epsps, pat, bar, CrylAb, CrylAc, Cry3Bb1 , Cry2Ab, Cry1 F, Cry34Ab1 and Cry35Ab1 . I /

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant expresses one or more genes selected from CP4 epsps, pat, bar, CrylAb, CrylAc, Cry3Bb1 , Cry2Ab, Cry1 F, Cry34Ab1 and Cry35Ab1 .

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, wherein the plant expresses one or more genes selected from CP4 epsps, pat, bar, CrylAb, CrylAc, Cry3Bb1 , Cry2Ab, Cryl F, Cry34Ab1 and Cry35Ab1 .

MGM-3.ln a further embodiments of the invention are those methods of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen or mixtures of afidopyropene with another pesticidal active ingredient, wherein the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, bla, bxn, cDHDPS, CP, cmv-cp, CrylAb, CrylAc, Cry1A.105, Cryl F, Cry1 Fa2, Cry2Ab, Cry34Ab1 , Cry35Ab1 , Cry3A, Cry3Bb1 , Cry9C, dam, DHFR, fad2, fanl , FH, flcrylAb, GAT4601 , GAT4602, gmFAD2-1 , GM-HRA, goxv247, gus, hel, mCry3A, nos, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A(a), wmv2-cp and zymv-cp.

In one embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating plant propagation materials, prefera- bly seeds with afidopyropen or mixtures comprising afidopyropen and another pesticidal active ingredient, wherein the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, bla, bxn, cDHDPS, CP, cmv-cp, CrylAb, CrylAc, Cry1A.105, Cryl F, Cry1 Fa2, Cry2Ab, Cry34Ab1 , Cry35Ab1 , Cry3A, Cry3Bb1 , Cry9C, dam, DHFR, fad2, fanl , FH, flcrylAb, GAT4601 , GAT4602, gmFAD2-1 , GM-HRA, goxv247, gus, hel, mCry3A, nos, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A(a), wmv2-cp and zymv-cp.

In such method, the afidopyropen is preferably applied in combination with abamectin, dinote- furan, ethiprole und fipronil. In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of cultivated plants by treating cultivated plants, parts of such plants or at their locus of growth with afidopyropen, wherein the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, bla, bxn, cDHDPS, CP, cmv-cp, CrylAb, CrylAc, Cry1A.105, Cryl F, Cry1 Fa2, Cry2Ab, Cry34Ab1 , Cry35Ab1 , Cry3A, Cry3Bb1 , Cry9C, dam, DHFR, fad2, fanl , FH, flcrylAb,

GAT4601 , GAT4602, gmFAD2-1 , GM-HRA, goxv247, gus, hel, mCry3A, nos, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A(a), wmv2-cp and zymv-cp. _Λ _, _Λ

174

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating cultivated plants, parts of such plants or at their locus of growth with a mixture comprising afidopyropen and abamectin, wherein the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, bla, bxn, cDHDPS, CP, cmv-cp, CrylAb, CrylAc, Cry1A.105, Cry1 F, Cry1 Fa2, Cry2Ab, Cry34Ab1 , Cry35Ab1 , Cry3A, Cry3Bb1 , Cry9C, dam, DHFR, fad2, fanl , FH, flcrylAb, GAT4601 , GAT4602, gmFAD2-1 , GM-HRA, goxv247, gus, hel, mCry3A, nos, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A(a), wmv2-cp and zymv-cp.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and dinotefuran, wherein the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, bla, bxn, cDHDPS, CP, cmv-cp, CrylAb, CrylAc, Cry1A.105, Cry1 F, Cry1 Fa2, Cry2Ab, Cry34Ab1 , Cry35Ab1 , Cry3A, Cry3Bb1 , Cry9C, dam, DHFR, fad2, fanl , FH, flcrylAb, GAT4601 , GAT4602, gmFAD2-1 , GM-HRA, goxv247, gus, hel, mCry3A, nos, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A(a), wmv2-cp and zymv-cp.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and fipronil, wherein the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, bla, bxn, cDHDPS, CP, cmv-cp, CrylAb, CrylAc, Cry1A.105, Cry1 F, Cry1 Fa2, Cry2Ab, Cry34Ab1 , Cry35Ab1 , Cry3A, Cry3Bb1 , Cry9C, dam, DHFR, fad2, fanl , FH, flcrylAb, GAT4601 , GAT4602, gmFAD2-1 , GM-HRA, goxv247, gus, hel, mCry3A, nos, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A(a), wmv2-cp and zymv-cp.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and ethiprole, wherein the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, bla, bxn, cDHDPS, CP, cmv-cp, CrylAb, CrylAc, Cry1A.105, Cry1 F, Cry1 Fa2, Cry2Ab, Cry34Ab1 , Cry35Ab1 , Cry3A, Cry3Bb1 , Cry9C, dam, DHFR, fad2, fanl , FH, flcrylAb, GAT4601 , GAT4602, gmFAD2-1 , GM-HRA, goxv247, gus, hel, mCry3A, nos, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A(a), wmv2-cp and zymv-cp.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and chlorfenapyr, wherein the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, bla, bxn, cDHDPS, CP, cmv-cp, CrylAb, CrylAc, Cry1A.105, Cry1 F, Cry1 Fa2, Cry2Ab, Cry34Ab1 , Cry35Ab1 , Cry3A, Cry3Bb1 , Cry9C, dam, DHFR, fad2, fanl , FH, flcrylAb, GAT4601 , GAT4602, gmFAD2-1 , GM-HRA, goxv247, gus, hel, mCry3A, nos, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A(a), wmv2-cp and zymv-cp.

In one more preferred embodiment, the present invention relates to a method of controlling harmful insects and/or increasing the health of plants by treating plant propagation materials, preferably seeds of cultivated plants of cultivated crops with a mixture comprising afidopyropen and a-cypermethrin, , wherein the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, bla, bxn, cDHDPS, CP, cmv-cp, CrylAb, CrylAc, Cry1A.105, Cry1 F, Cry1 Fa2, Cry2Ab, Cry34Ab1 , Cry35Ab1 , Cry3A, Cry3Bb1 , Cry9C, dam, DHFR, fad2, fanl , FH, flcrylAb, GAT4601 , GAT4602, gmFAD2-1 , GM-HRA, goxv247, gus, hel, mCry3A, nos, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A(a), wmv2-cp and zymv-cp.

Examples

The present invention may be illustrated in further detail by the following example. B. Biology

Synergism can be described as an interaction where the combined effect of two or more com- pounds is greater than the sum of the individual effects of each of the compounds. The presence of a synergistic effect in terms of percent control, between two mixing partners (X and Y) can be calculated using the Colby equation (Colby, S. R., 1967, Calculating Synergistic and Antagonistic Responses in Herbicide Combinations, Weeds, 15, 20-22):

XY

E = X + Y

100

When the observed combined control effect is greater than the expected combined control effect (E), then the combined effect is synergistic. The following test may be used to demonstrate the control efficacy of compound, mixtures or compositions of this invention on specific pests. However, the pest control protection afforded by the compound, mixtures or compositions is not limited to the species described. In certain instances, combinations of a compound of this invention with other invertebrate pest control compounds or agents are found to exhibit synergistic effects against certain important inverte- brate pests. _Λ _,_η

176

The analysis of synergism or antagonism between the mixtures or compositions is determined using Colby's equation.

B1 : Test on GMO soybeans

Trial is carried out under greenhouse conditions on soybean (GMO plant variety, e.g. roundup, growth stage 109). 12 treatments are compared in a complete randomized blocks (4 replications) with plot size of 1 m x 3 meters. Only selected plants are considered for artificial infestation and evaluations.

Due to glyphosate timing for application on GMO-soybeans, all treatments are to be applied in older plants (GS 109) otherwise a significant phytotoxicity would be expected. Application is done, using 400 l/ha. All treatments are applied using a CO2 backpack (nozzle type TXVK-10). Temperature at the time of applications is around 25 to 30 °C and air humidity is between 30 and 100%. Soil condition is e.g. R4 (when <75% of surface is dried up) and the moisture is moist (normal).

Roundup Original ® (Glyfosate-sal isopropilamina @360g/L) is used in the rate of 867 g a.i./ha. Artificial infestation is done one day after the application. The species used is Ant/cars/a gem- matalis (Hubner) [ Thermesia elegantula (Herrich-Schaffer, 1869)], Noctuidae. 5 plants/plot are infested with 3 larvae (stage L2) using a entomological metallic tweezers, totaling 15 larvae per repetition. Larvae used in this trial are e.g. provided by BASF rearing laboratory, Campinas, Brazil.

A second infestation is held seven days after application in the same plants and using the same larval numbers. A third infestation might be done if necessary in order to observe residual activity.

The mortality (number) and eating damage (%) are evaluated with 01 , 02, 05, 07, 14 and 21 DAA (days after application), comparing to untreated control plants.

Increased mortalities in combination with the application of roundup can be observed when compared to the untreated control plants:

Additionally, after 5 days after application at 12.5 g a.i./ha a reduction of feeding damage compared to the untreated controls can be observed. In another test, a non-GM soybean variety is treated with 12.5 g a.i./ha and showed less reduction in feeding damage compared to the un- treated controls.

Claims

Claims

1 . A method for controlling pests and/or increasing the plant health of a cultivated plant as compared to the respective non-modified control plant, comprising the application of afidopyropen or a mixture comprising afidopyropen with another pesticidal active ingredient to a plant with at least one modification, parts of such plant, plant propagation material, or to its locus of growth.

2. The method according to claim 1 , wherein the cultivated plant is a plant with at least one modified property selected from: herbicide tolerance, insect resistance, fungal resistance or viral resistance or bacterial resistance, stress tolerance, maturation alteration, content modification of chemicals present in the cultivated plant, modified nutrient uptake, antibiotic resistance or male sterility compared to the corresponding control plant respectively.

3. The method according to claim 1 or 2, wherein the yield of the cultivated plant is increased.

4. The method according to any one of claims 1 to 3, wherein the cultivated plant is

(i) a plant with at least one trait of the category herbicide tolerance, or

(ii) a plant with at least one trait of the category insect resistance, or

(iii) a plant with at least two traits, wherein at least one trait is of the category of herbicide tolerance and at least one trait is of the category of insect resistance.

5. The method according to any one of claims 2, 3, 4 or 5, wherein the herbicide resistance is selected from the group consisting of glyphosate tolerance, glufosinate tolerance, and imidazolinone tolerance, and is particularly preferably glyphosate tolerance.

6. The method according to any one of claims 2, 3, 4, or 5, wherein the insect resistance is selected from the group consisting of lepidopteran resistance and coleopteran resistance, and is particularly preferably lepidopteran resistance.

7. The method according to any one of claims 1 to 6, wherein the cultivated plant is a plant with insect resistance, wherein at least two genes confer insect resistance to the cultivated plant.

8. The method according to any one of claims 1 to 7, wherein the cultivated plant is selected from a row crop.

9. The method according to any one of claims 1 to 7, wherein the cultivated plant is selected from a specialty crop.

10. The method according to any one of claims 1 to 9, wherein the cultivated plant is selected from the group consisting of Gossypium hirsutum L. (cotton), Zea mays L. (maize or corn), Glycine max L. (soybean), Triticum aestivum (wheat), Oryza sativa (Rice), Solanum tuberosum (potato), Solanum lycopersicum (tomoato), Lycopersicon esculentum (tomato) and Oryza sativa L (rice), preferably from the group consisting of Gossypium hirsutum L. (cotton), Zea mays L. (maize), Solanum lycopersicum (tomato), Lycopersicon esculentum (to- mato) and Glycine max L. (soybean).

1 1 . A method for controlling pests and/or increasing the plant health of a cultivated plant as compared to the respective non-modified control plant, comprising the application of afidopyropen or a mixture comprising afidopyropen with another pesticidal active ingre- dient , to a plant with at least one modification, parts of such plant, plant propagation material, or at its locus of growth, wherein the cultivated plant is

(i) Gossypium hirsutum L. (cotton) or Zea mays L. (maize) and comprises at least one gene selected from the group consisting of dvsnf7, cry1A, cry1Ab-Ac, cry1 C, cry2Ab2, cry2Ae, mocryl F; or

(ii) Glycine max L. (soybean), Triticum aestivum (wheat) or Oryza sativa L. (rice) and comprises at least one gene selected from the group consisting of cry34Ab1 , cry35 Ab1 , cry3A, cry3Bb1 , dvsnf7, mcry3A, cry1A, cry1A.105, crylAb, crylAb- Ac, crylAc, cry1 C, cry1 F, cry1 Fa2, cry2Ab2, cry2Ae, cry9c, mocryl F, pin 11 , vip3A(a), vip3Aa20.

12. Method according to any of claims 1 to 1 1 , wherein the cultivated plant is a soybean variety with glyphosate tolerance and lepidopteran resistance.

13. Method according to any of claims 1 to 12, wherein the glyphosate tolerance is through the expression of the cp4epsps gene, or wherein the lepidopteran resistance is through ex- presssion of the Cry1 AC encoding gene from B. thuringiensis, or wherein both the glyphosate tolerance is through the expression of the cp4epsps gene and the lepidopteran resistance is through expresssion of the Cry1 AC encoding gene from B. thuringiensis. 14. Method according to any of claims 1 to 13, wherein the glyphosate tolerance is based on the transgenic event MON89788, or wherein the lepidopteran resistance is based on the transgenic event MON87701 , or wherein both the glyphosate tolerance is based on the transgenic event MON89788 and the lepidopteran resistance is based on the transgenic event MON87701 .

15. Method according to any of claims 1 to 14, wherein the lepidopteran resistance is against a species selected from the group of velvetbean caterpillar (Anticarsia gemmatalis) and soybean looper (Pseudop/usia includens, Chrysodeixis includens ). 16. The method according to any of claims 1 -14, wherein the cultivated plant provides tolerance to glyphosate herbicide and protection against major soybean pests (velvetbean caterpilar, soybean looper, soybean budborer, bean shoot borer, bollworm, corn stalk borer, Helicoverpa, e.g. Helicoverpa armigera) and optionally also increased yield potential, and which cultivated plant is preferably "Intacta RR2 PRO™" soybean.

A method of controlling harmful insects by treating cultivated plants, parts of such plants or their locus of growth with afidopyropen or a mixture comprising afidopyropene with another pesticidal active ingredient selected from insecticides, wherein the plant has at least one insecticidal trait, and wherein the harmful insects are resistant to that at least one insecticidal trait of the plant.

The method according to any one of claims 1 to 17, wherein afidopyropen or a mixture comprising afidopyropene with another pesticidal active ingredient is applied to the plant propagation material, preferably seeds, of the cultivated plant.

The method according to any one of claims 1 to 18, wherein the treatment(s) are carried out by applying afidopyropen or a mixture comprising afidopyropene with another pesticidal active ingredient to the plant with at least one modification, parts of the cultivated plant or to their habitat.

The method according to any one of claims 1 to 19, wherein, when a mixture of afidopyropen is applied, the other active ingredient is an insecticide selected from fipronil,ethiprole, a-cypermethrin, dinotefuran, chlorfenapyr, abamectin, the carboxamid 3-[benzoyl(methyl)amino]-N-[2-bromo-4-[1 ,2,2,2-tetrafluoro-1 -(trifluoromethyl)ethyl]-6- (trifluoromethyl)phenyl]-2-fluoro-benzamide or a ryanodine receptor modulator selected from N-[4,6-dichloro-2-[(diethyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2-(3-chloro- 2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide, N-[4-chloro-2-[(diethyl-lambda-4- sulfanylidene)carbamoyl]-6-methyl-phenyl]-2-(3-chloro-2-pyridyl)-5- (trifluoromethyl)pyrazole-3-carboxamide or N-[4-chloro-2-[(di-2-propyl-lambda-4- sulfanylidene)carbamoyl]-6-methyl-phenyl]-2-(3-chloro-2-pyridyl)-5- (trifluoromethyl)pyrazole-3-carboxamide .

21 . The method according to any of claims 1 to 20, additionally comprising the application of a herbicide, to which the plant is tolerant.

Seed of a cultivated plant as defined in any one of claims 1 to 8, 9, 10 or 1 1 , which is treated afidopyropen or a mixture comprising afidopyropene with another pesticidal active ingredient.

23. Seed according to claim 17, wherein, when the seed has been treated with a mixture comprising afidopyropene with another pesticidal active ingredient, the other pesticidal active ingredient is selected from abamectin, dinotefuran, fipronil or ethiprole.