KR101457996B1 - Antibacterial composition containg staphylea sp.exctract - Google Patents

Abstract

The present invention relates to an antimicrobial composition comprising a natural extract.

Description

ANTIBACTERIAL COMPOSITION CONTAINING STAPHYLEA SP.EXCTRACT <br> <br> <br> Patents - stay tuned to the technology ANTIBACTERIAL COMPOSITION CONTAINING STAPHYLEA SP.EXCTRACT

The present invention relates to an antimicrobial composition comprising a natural extract.

Antibiotics are substances with selective toxicity that kill microorganisms and are not toxic to humans or animals and are not inactivated by enzymes in the body. They are mainly DNA replication, transcription and decryption of genetic information, Transport, and biosynthesis of the cell wall, thereby exhibiting an effect through a mechanism of inhibiting the proliferation of microorganisms.

Until now, the main antibiotics could be chemically synthesized with those produced by microorganisms. The first antibiotic produced by this method was penicillin, discovered by Alexander Fleming in 1928, which was then largely liberated from microbial disease. In the United States, about 2-3 million tons a year are produced, of which 50% is used for animal feed and the rest is used as medicines. However, European countries and the United States are strengthening regulations to prohibit antibiotics used for the purpose of promoting the growth of animals and strictly regulate the use of human beings only by a doctor's diagnosis. This is due to the emergence of resistant antibiotics against day-to-day spread of antibiotics, thus requiring safe medicines that can replace conventional antibiotics.

In addition, since antibiotics used in the past have a very complicated structure, they are not only difficult to synthesize, but also have a problem that their synthesis is also less economical than the fermentation method. Thus, discovery of a novel substance or its intermediate product from a natural product or altering its substitution will be useful for obtaining new antibiotics.

Therefore, the inventors of the present invention have continued to study an antimicrobial composition having therapeutic and preventive effects against harmful bacteria that we encounter in everyday life. As a result, the present inventors have found that the antimicrobial activity is excellent, And the present invention was completed.

Published Korean Patent Application No. 2003-0020908

It is an object of the present invention to provide a composition having antibacterial properties and being harmless to an organism.

In order to achieve the above object,Staphylea sp.) plant extract as an effective ingredient.

The antimicrobial composition of the present invention has an antibacterial activity against bacteria, specifically bacteria on the skin surface. Therefore, it has antimicrobial activity against skin diseases such as atopy, ringworm, or psoriasis, and therefore, it can be used for therapeutic treatment.

Furthermore, the antimicrobial composition of the present invention contains a natural plant-derived component as an active ingredient, and has little side effects and is natural-friendly.

Fig. 1 shows the results of the disk diffusion method of Experimental Example 1. Fig.

The present invention relates, in one aspect, to a pharmaceutical composition comprising Staphylea sp.) plant extract as an active ingredient.

The antimicrobial composition which is one aspect of the present invention may contain an extract or fraction of a plant belonging to the genus Staphylococcus as an active ingredient.

In the antimicrobial composition according to one aspect of the present invention, the Staphylea sp. Plant is Staphylea bolanderi, Staphylea bumalda. Staphylea colchica, Staphylea emodi, Staphylea forrestii, Staphylea holocarpa, Staphylea pinnata, Staphylla pinnata, Staphylea pringlei, Staphylea shweliensis, and Staphylea trifolia. In addition, the present invention is not limited thereto.

The Staphylea bolanderi is a species of red pepper tree grown in the northwest region of the United States and Staphylea bumalda is a species of red pepper tree grown in East Asia. In addition, Staphylea colchica is a species of red pepper tree grown in the Himalayas in Southwest Asia and Staphylea emodi. Staphylea forrestii and Staphylea holocarpa are species of red pepper trees grown in China. Staphylea pinnata is in Europe, and Staphylea pringlei is a pepper tree grown in Mexico. Staphylea shweliensis is a Chinese red pepper tree, and Staphylea trifolia is a red pepper tree grown in the Northeast of the United States.

Specifically, in the antimicrobial composition which is one aspect of the present invention, the above-mentioned Staphylea sp.) The plant may be a pepper tree (Staphylea bumalda).

The pepper tree ( Staphylea bumalda ) is a deciduous broad-leaved arboreous tree belonging to the Staphyleaceae family. Young leaves are edible and wood is used as pegs, wooden chopsticks or firewood.

As used herein, the plant of the genus Staphylla for the production of the extract or fraction is not limited to the entire range of plants such as its roots, fruit, stem or leaf. Specifically, the fruit of the plant of the genus Staphylococcus may be used for the production of the extract or the fraction.

In addition, in the present specification, the plant belonging to the genus Staphylla may be cultivated or commercially available.

The extract may contain a Staphylococcus plant-C ₁ -C ₆ alcohol extract, and may specifically include a Staphylococcus aureus-methanol extract or a Staphylococcus aureus-ethanol extract.

As used herein, the extract of the Staphylococcus species may be a crude extract of a solvent selected from the group consisting of water, C ₁ -C ₆ alcohols, and combinations thereof. The C ₁ -C ₆ alcohol may specifically be methanol or ethanol. When the plant of the genus Staphylococcus, specifically the pepper tree is extracted with a solvent, it is preferable to extract the plant by adding about 5 to about 15 times the amount of the above-mentioned plant, specifically about 10 times the amount of the solvent But is not limited thereto. The extraction may be performed by heat extraction, cold extraction, reflux cooling extraction, ultrasonic extraction or the like, and there is no limitation as long as it is an extraction method that is obvious to a person skilled in the art. The extraction may be carried out at room temperature, but it may be carried out under heating at a temperature of about 40 to 100 ° C, more preferably about 80 ° C, for a more efficient extraction, It is not. The extraction time is preferably about 2 to 4 hours, more preferably about 3 hours, but it is not limited thereto and may be varied depending on conditions such as extraction solvent and extraction temperature. The extraction may be carried out one or more times several times to obtain a larger amount of the active ingredient, preferably 1 to 5 times, more preferably 3 times of continuous extraction.

In the present specification, the plant extract of Staphylococcus aureus may contain crude extract of the plant belonging to the genus Staphylococcus, as described above, and may be included as a soluble fraction of an organic solvent obtained by further extracting the crude extract with an organic solvent having a low polarity have. Examples of the organic solvent include, but are not limited to, hexane, methylene chloride, ethyl acetate, n-butanol, and the like. The extract or the soluble fraction of the extract may be used as it is, or may be used as an extract form by concentration after filtration, and may be used as a form of a lyophilizate by concentration and freeze-drying.

It is preferred, but not limited, that the Staphylococcus sp. Plant, specifically the pepper tree extract, is prepared by the following steps:

1) extracting the dried plant by adding an extraction solvent;

2) filtering the extract of step 1);

3) Step of extracting the filtered extract of step 2) by concentration under reduced pressure.

The extraction solvent is preferably a solvent selected from water, an alcohol or a mixture thereof, preferably a C ₁ to C ₄ lower alcohol or a mixed solvent thereof. More preferably, the extraction solvent is methanol or ethanol, But is not limited thereto. The amount of the extraction solvent is preferably 0.5 to 100 times, more preferably 5 to 10 times, the dry weight of the plant of the genus Staphylococcus, specifically the pepper tree, but is not limited thereto. The extraction method may be a conventional extraction method such as supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, hot water extraction, immersion extraction, reflux cooling extraction and ultrasonic extraction. Preferably, &Lt; / RTI &gt; The temperature for extraction is preferably from 10 to 100 ° C, more preferably room temperature, but is not limited thereto. The extraction time is preferably 2 hours to 24 hours, more preferably 3 hours, but is not limited thereto.

In the above method, it is preferable, but not limited, to use a vacuum rotary evaporator for the vacuum concentration in step 3). The drying is preferably, but not exclusively, reduced-pressure drying, vacuum drying, boiling drying, spray drying, room temperature drying or freeze-drying.

The fraction of the plant of the present invention, particularly the pepper tree, is preferably, but not limited to, the following steps in the preparation of the extract:

4) Extracting the extract of step 3) with an organic solvent to prepare fractions.

In the above method, the organic solvent in step 4) is preferably n-hexane, dichloromethane and ethyl acetate, more preferably ethyl acetate, but not limited thereto Do not.

In this method, the fraction of step 4) was prepared by suspending a 70% ethanol extract in distilled water, mixing the same amount of n-hexane, separating the hexane layer by adding a separating funnel, The dichloromethane layer was separated and removed, and the remaining water layer was again mixed with an equal volume of ethyl acetate. The ethyl acetate layer was separated, concentrated, lyophilized, and dissolved in ethyl acetate It is preferable to prepare a fraction. The fraction may be obtained by repeating the fractionation process from 1 to 5 times, preferably 3 times, from the extract, followed by fractionation and concentration under reduced pressure. However, the fraction is not limited thereto.

In a specific example of the present invention, the disk diffusion method was performed to confirm the antibacterial activity of the plant extract of Staphylococcus aureus of the present invention. As a result, it was found that the plant extract of Staphylococcus epidermidis exhibits a broad transparent zone or high antimicrobial activity with little growth of bacteria.

In the antimicrobial composition according to one aspect of the present invention, the bacterium may specifically include a skin pathogenic bacterium. More specifically, the bacterium may be selected from the group consisting of Candida albicans , S. cerevisiae, S. cerevisiae, and the like . S. cerevisiae , E. coli , B. subtilis , S. aureus , E. hirae , and M. corus luteus . luteus , methicillin resistant staphylococcus aureus (MRSA), Propionibacterium acnes , Epidermophyton floccosum , microspheres agodawinii Microsporum audouinii , Trichophyton ferrugineum , Trichophyton mentagrophytes , Trichophyton ruburm , Staphylococcus aureus , , Staphylococcus epidermis , Pityrosporum ovale , Candida albicans , and Trichomonas vaginalis. In addition, the present invention relates to a method for producing a microorganism, More specifically, according to one aspect of the compositions of the present invention, the bacteria is Staphylococcus fringing-house (S. aureus) and Staphylococcus epidermidis (Staphylococcus epidermis) may be one or more selected from the group consisting of homogeneous.

In the antimicrobial composition according to one aspect of the present invention, the composition may contain 0.02 to 50% by weight of a plant extract of Staphylla sp., Based on the total weight of the composition. It is necessary to contain an extract of Staphylococcus aureus within the above range to have antimicrobial activity. That is, when the extract of Staphylococcus aureus is contained in an amount of less than 0.02% by weight based on the total weight of the composition, the antimicrobial activity against the bacterium is not observed, and the extract of Staphylococcus aureus is 50% by weight If it is contained in excess, it affects the composition ratio of the other contained substance. In view of the above, the composition of the present invention may contain 0.02 to 50 wt%, 0.02 to 50 wt%, 0.02 to 50 wt%, 0.02 to 50 wt%, 0.02 to 50 wt% of the stapleaf plant extract, By weight or 0.02 to 50% by weight of a plant extract of Staphylococcus aureus.

In the antimicrobial composition according to one aspect of the present invention, the Staphylea sp. Plant extract includes a staphylococcus plant-C ₁ -C ₆ alcohol extract.

In the antimicrobial composition which is one aspect of the present invention, the alcohol may be methanol or ethanol.

In the antimicrobial composition which is one aspect of the present invention, the composition may be a cosmetic composition.

The formulation of the cosmetic composition is not particularly limited and may be appropriately selected according to the purpose. For example, it can be applied to softeners (skin lotion and milk lotion), nutrition lotion, essence, nutrition cream, massage cream, pack, gel, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, A body lotion, a body cream, a body oil, and a body essence, but the present invention is not limited thereto. In addition, the cosmetic composition may be used in the form of an external preparation for skin in the form of ointment, patch, or the like.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, so long as they do not impair the main effect. The cosmetic composition according to the present invention may further comprise a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by those skilled in the art within a range not to impair the objects and effects of the present invention. The amount thereof may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, have.

In the antimicrobial composition which is one aspect of the present invention, the composition includes a health food composition.

The health food composition of the present invention provides various forms of food additives or functional foods. The composition may be processed into an extruded tea, a liquid tea, a beverage, a fermented milk, a cheese, a yogurt, a juice, a probiotic agent, a health supplement, and the like, and various other food additives.

In one embodiment, the composition may contain other ingredients or the like that can give synergistic effects to the main effect within a range that does not impair the intended main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention. For example, the addition amount of the above components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.

The composition according to the present invention may be in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, etc., and may be administered by various methods such as simple drinking, injection administration, spraying or squeezing.

In the antimicrobial composition which is one aspect of the present invention, the composition includes a pharmaceutical composition.

The formulation of the pharmaceutical composition according to the present invention may be a solution, a suspension, a milk, a gel, a drip agent, a suppository, a patch or a spray agent, but is not limited thereto. The formulations can be readily prepared according to conventional methods in the art and can be prepared by conventional means such as excipients, wetting agents, emulsifying accelerators, suspending agents, salts or buffers for controlling osmotic pressure, coloring agents, spices, stabilizers, preservatives, Adjuvants may be used as appropriate.

The effective ingredients of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be treated, administration route or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, 0.000025 mg / g / day to 0.025 mg / g / day, more specifically 0.00025 mg / g / day. &lt; / RTI &gt;

The pharmaceutical composition of the present invention may be administered orally or transdermally, but is not limited thereto.

In the antimicrobial composition which is one aspect of the present invention, the composition may be a composition for external application to the skin. Specifically, the composition for external application for skin includes a solution, a suspension, a spray, a patch, a pad, a cream, an ointment and a gel.

Hereinafter, the present invention will be described in detail with reference to Examples and Production Examples. However, the following examples and preparative examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention.

< Manufacturing example  1> Preparation of Extracts and Preparation of Strain for Antibacterial Experiment

The methanol extracts of red pepper were purchased from Plant Extract Bank, Korea Research Institute of Bioscience and Biotechnology. Each extract was dissolved in dimethyl sulfoxide (DMSO) and used for the experiment.

Pathogenic strains of Staphylococcus Arenas mouse (S. aureus) and Staphylococcus epidermidis (S. epidermidis) were used in the experiment received pre-sale from South Korea Genetic Resource Bank (KCTC).

< Experimental Example  1> Antibacterial test by disk diffusion method

In order to confirm the antimicrobial activity of the pepper tree extract of Example 1 prepared by the above method, the disk diffusion method was performed as described below.

Diluted 24hr Staphylococcus fringing-house (S. aureus), Staphylococcus epidermidis (S. epidermidis) grown in liquid medium by a factor of 10 for each, and stretch well laid ssikeul 200 ㎕ of them in an agar medium. The round filter paper having a diameter of about 8 mm was impregnated with 3 mu l of the solution dissolved in DMSO so that the concentration of the extract prepared in Example 1 was 20 mg / ml, and the solution was spread on an agar medium and spread on an agar medium. The antimicrobial activity was measured by measuring the clear zone in which bacterial growth was inhibited after incubation at 37 ° C for 24 hours. The results are shown in Table 1 below.

Control * Pepper tree S. aureus - ++ S. epidermidis - +

* Control: DMSO

** -: No suppression effect, +: less than 1mm, ++: more than 1mm, less than 2mm, +++: more than 2mm

As can be seen from Table 1, it was confirmed that the pepper tree extract had antimicrobial properties by forming a transparent ring for all the above-mentioned bacteria. Therefore, the extract of Ganoderma lucidum according to the present invention is excellent in antimicrobial activity and has no side effects, so that it can be effectively used for the purpose of removing bacteria.

Accordingly, the Staphylea of the present invention sp.) Plant extracts also have excellent antimicrobial activity and are useful for antimicrobial use because they have no side effects.

Hereinafter, a formulation example of the composition according to the present invention will be described. However, the pharmaceutical composition, the cosmetic composition or the health food composition can be applied to various formulations, and the present invention is not limited thereto but is specifically described.

[Formulation Example 1] Softening lotion (skin lotion)

Compounding ingredient Content (% by weight) Staphylococcus aureus plant extract 0.0025 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 2] Nutritional lotion (Milk lotion)

Compounding ingredient Content (% by weight) Staphylococcus aureus plant extract 0.0025 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 3] Nourishing cream

Compounding ingredient Content (% by weight) Staphylococcus aureus plant extract 0.0025 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 4] Massage cream

Compounding ingredient Content (% by weight) Staphylococcus aureus plant extract 0.0025 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Pack

Compounding ingredient Content (% by weight) Staphylococcus aureus plant extract 0.0025 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Good Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] ointment

Compounding ingredient Content (% by weight) Staphylococcus aureus plant extract 0.0025 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 1] Soft capsule

400 mg / capsule was mixed with 0.0025 g of a plant extract of Staphylococcus aureus, 50 mg of red ginseng extract, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow pearls and 6 mg of lecithin. .

[Formulation Example 2] Tablets

The mixture was mixed with 0.0025 g of a plant extract of Staphylococcus aureus, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate, and 40 mg of 30% ethanol was added thereto to form granules. And then the tablet was tableted.

[Formulation Example 3] Granules

The granules were formed by mixing 150 mg of the plant extract of Staphylococcus aureus, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch and 100 mg of 30% ethanol, and dried at 60 ° C. to form granules. Respectively. The final weight of the contents was 1 g.

[Formulation Example 4] Drinking agent

0.0025 g of Staphylococcus aureus plant extract, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled in a bottle. The final volume of the contents was adjusted to 200 ml.

[Formulation Example 5] Preparation of health food

Staphylococcus sp. Plant extract ............................ 0.0025g

Vitamin mixture

Vitamin A Acetate ....................... 70 ㎍

Vitamin E ............................................ 1.0 mg

Vitamin B1 ........................................... 0.13 mg

Vitamin B2 .......................................... 0.15 mg

Vitamin B6 ........................................... 0.5 mg

Vitamin B12 .............................. 0.2 g

Vitamin C ............................................. 10 mg

Biotin ................................................. 10 [mu] g

Nicotinic acid amide .................................. 1.7 mg

Folic acid ................................................. ..... 50 μg

Calcium pantothenate .................................... 0.5 mg

Mineral mixture

Ferrous sulfate .......................................... 1.75 mg

Zinc oxide ............................................ 0.82 mg

Magnesium carbonate ...................................... 25.3 mg

Potassium Phosphate ......................................... 15 mg

Secondary calcium phosphate ...................................... 55 mg

Potassium citrate ............................................ 90 mg

Calcium carbonate .............................................. 100 mg

Magnesium chloride ..................................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

[Formulation Example 6] Preparation of health drink

Staphylococcus sp. Plant extract .......................... 0.0025g

Citric acid ................................................. ... 1000 mg

oligosaccharide................................................. .... 100 g

Plum concentrate ................................................ ..... 2 g

Taurine ................................................. ........... 1 g

Purified water was added to the mixture to complete 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, &Lt; / RTI &gt;

Although the composition ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the blending ratio according to the regional or national preference such as the demand level, the demanding country, the use purpose, and the like. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (10)

A composition for external application for skin for antimicrobial use which comprises a plant extract of Staphylla sp. As an active ingredient,
The bacterium may be selected from the group consisting of Candida albicans , S. cerevisiae , E. coli , B. subtilis , E ( E. coli ), B. subtilis . hirae), the micro base Lactococcus Proteus (M. luteus), methicillin-resistant Staphylococcus fringing mouse (MRSA; methicillin resistant staphylococcus aureus) , propynyl sludge tumefaciens arc Ness (Propionibacterium acnes), epi more fur tone floc koseom (Epidermophyton microsporum audouinii , Trichophyton ferrugineum , Trichophyton mentagrophytes , Trichophyton ruburm , Staphylococcus aureus, Staphylococcus aureus, Staphylococcus aureus , Staphylococcus epidermis , Pityrosporum ovale , Candida albicans , and Trichomonas vaginalis ). &Lt; / RTI &gt; The composition for external application for skin for antimicrobial use according to claim 1, wherein the skin surface bacteria is at least one selected from the group consisting of The method according to claim 1,
The Staphylea sp. Plants may be selected from the group consisting of Staphylea bolanderi , Staphylea bumalda , Staphylea colchica , Staphylea emodi , Staphylea forrestii , Staphylea holocarpa , Staphylea pinnata , Staphylea pringlei , Staphylea shweliensis , and Staphylla spp . Wherein the composition is at least one selected from the group consisting of Staphylla trifolia . The method according to claim 1,
The star LEA in blood (Staphylea sp.) Plants are red pepper tree (Staphylea bumalda) an antimicrobial composition for external application for skin.
delete The method according to claim 1,
Wherein the composition comprises 0.02 to 50% by weight of a plant extract of Staphylea sp. Based on the total weight of the composition. The method according to claim 1,
Wherein the Staphylea sp. Plant extract is a Staphylococcus aureus- C ₁ -C ₆ alcohol extract. The method according to claim 6,
Wherein the alcohol is methanol or ethanol. delete delete delete

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